CN108083451A - A kind of compound bacteria microcapsule water purification agent of slow-release and preparation method thereof - Google Patents
A kind of compound bacteria microcapsule water purification agent of slow-release and preparation method thereof Download PDFInfo
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- CN108083451A CN108083451A CN201711316117.0A CN201711316117A CN108083451A CN 108083451 A CN108083451 A CN 108083451A CN 201711316117 A CN201711316117 A CN 201711316117A CN 108083451 A CN108083451 A CN 108083451A
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
- C02F3/348—Biological treatment of water, waste water, or sewage characterised by the microorganisms used characterised by the way or the form in which the microorganisms are added or dosed
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
- B01J13/00—Colloid chemistry, e.g. the production of colloidal materials or their solutions, not otherwise provided for; Making microcapsules or microballoons
- B01J13/02—Making microcapsules or microballoons
- B01J13/025—Applications of microcapsules not provided for in other subclasses
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
- B01J13/00—Colloid chemistry, e.g. the production of colloidal materials or their solutions, not otherwise provided for; Making microcapsules or microballoons
- B01J13/02—Making microcapsules or microballoons
- B01J13/04—Making microcapsules or microballoons by physical processes, e.g. drying, spraying
- B01J13/043—Drying and spraying
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
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Abstract
The present invention provides a kind of compound bacteria microcapsule water purification agents of slow-release, zymotic fluid including microcapsules and compound bacteria, the compound bacteria is polyP bacteria, nitrobacteria, photosynthetic bacteria and bacillus subtilis, and the microcapsules include the following component based on concentrated broth weight percent:21 ‰~25 ‰ ethyl cellulose, 0.8 ‰~1.2 ‰ casein-sodium, 0.9 ‰~1.3 ‰ Tween 80,0.5 ‰~1.0 ‰ poloxamer, 0.2 ‰~0.5 ‰ gelatin.The compound bacteria microcapsule water purification agent of the slow-release is wrapped up compound bacteria by microcapsules, compound bacteria is isolated with external environment, improve bacterial activity, the compound bacteria microcapsule water purification agent of the slow-release can slowly release purifying water micrcxDrganism in water simultaneously, play lasting water purification effect, and can mutually act synergistically between a variety of strains of compound bacteria, improve purification efficiency.
Description
Technical field
The invention belongs to ecological, environmental protective technical fields, and in particular to a kind of compound bacteria microcapsule water purification agent of slow-release and its system
Preparation Method, for being purified to water body.
Background technology
PolyP bacteria (PAO) is a kind of special bacterium in conventional activated sludge process, can be incited somebody to action to excess under aerobic state
In phosphorus suction body in sewage, make the several times that in vivo phosphorus content is more than the in vivo phosphorus content of general bacterium, this kind of bacterium is wide
Biological phosphate-eliminating is used for generally.Nitrobacteria (Nitrifying bacteria) is a kind of aerobic bacteria, including nitrous acid
Bacterium and Nitromonas, nitrobacteria have great importance for the cycling conversion of nitrogen in nature, can be oxidized to organic ammonia nitrogen
Cultured water or nitrate nitrogen, are then removed by denitrification, this plays an important role to the removal of Water pollution.It is photosynthetic
Bacterium (Photosynthetic Bacteria) is that occur generally existing in earliest, nature on the earth, there is original luminous energy to close
The prokaryotes of architectonical are the general names for not put the photosynthetic bacterium of oxygen under anaerobic, are that one kind is not formed
The Gram-negative bacteria of gemma ability is using light as the energy, nature can be utilized under anaerobism illumination or aerobic dark condition
In organic matter, sulfide, ammonia etc. carry out photosynthetic microorganism as hydrogen donor and carbon source;Photosynthetic bacteria applies water body
Afterwards, the organic particle in its degradable water body, moreover it is possible to absorb the nuisances such as ammonia, nitrite, the hydrogen sulfide in water body
Matter.Bacillus subtillis(Bacillus subtilis), be Bacillus one kind, individual cells 0.7~0.8 × 2~3
Micron, uniform coloring, no pod membrane, peritrichous can move, gram-positive bacteria, 0.6~0.9 × 1.0~1.5 microns of gemma,
Ellipse is to column, and central or slightly inclined positioned at thalline, thalline does not expand after sporulation;Bacillus subtilis has very strong cleaning
The short grained effect of rubbish in water has very strong suppression to harmful microorganisms such as vibrios, Escherichia coli and the baculovirals in aquatic products
It makes and uses, additionally it is possible to improve water turbidity problem caused by harmful cyanobacteria is overflowed.These four microbial association uses can play
Go out the purifying water effect of bigger.
Currently, domestic water pollution is increasingly severe, is had attracted much attention using the technology of biological cleaning water body, particularly micro- life
Object water purification microbial inoculum.However, traditional water purification microbial inoculum usually just all releases microorganism putting into the time very short in water
Come, purifying water effect is preferable in a short time, but is difficult to continue to play catharsis.
The content of the invention
The purpose of the present invention is overcome after effect time in existing water purification microbial inoculum input water short, it is difficult to lasting to play purification work
The problem of using.
For this purpose, the present invention provides a kind of compound bacteria microcapsule water purification agent of slow-release, the hair including microcapsules and compound bacteria
Zymotic fluid, the compound bacteria are polyP bacteria, nitrobacteria, photosynthetic bacteria and bacillus subtilis, and the microcapsules are included by concentration
The following component of zymotic fluid weight percent meter:21 ‰~25 ‰ ethyl cellulose, 0.8 ‰~1.2 ‰ casein-sodium,
0.9 ‰~1.3 ‰ Tween 80,0.5 ‰~1.0 ‰ poloxamer, 0.2 ‰~0.5 ‰ gelatin.
Further, above-mentioned microcapsules include the following component based on concentrated broth weight percent:23 ‰~25 ‰
Ethyl cellulose, 1.0 ‰~1.2 ‰ casein-sodium, 1.1 ‰~1.3 ‰ Tween 80,0.8 ‰~1.0 ‰ pool Lip river it is husky
Nurse, 0.4 ‰~0.5 ‰ gelatin.
Further, above-mentioned microcapsules include the following component based on concentrated broth weight percent:25 ‰ ethyl
Cellulose, 1.2 ‰ casein-sodium, 1.3 ‰ Tween 80,1.0 ‰ poloxamer, 0.5 ‰ gelatin.
In addition, the present invention also provides a kind of preparation method of the compound bacteria microcapsule water purification agent of slow-release, including walking as follows
Suddenly:
1) polyP bacteria, nitrobacteria, photosynthetic bacteria, bacillus subtilis is added in zymotic fluid to ferment;
2) solids in zymotic fluid is filtered out after the completion of step 1) fermentation, low temperature concentration is carried out to zymotic fluid;
3) in zymotic fluid after concentration, ethyl cellulose, casein-sodium, Tween 80, poloxamer, gelatin are added in, stirring is molten
The compound bacteria microcapsule water purification agent of slow-release is made through emulsifying, being spray-dried after solution is uniform.
Further, above-mentioned steps 1) in polyP bacteria strain and zymotic fluid mass ratio be 0.5~0.7 ‰, nitrobacteria bacterium
Kind is 0.3~0.4 ‰ with zymotic fluid mass ratio, and photosynthetic bacteria strain is 0.1~0.2 ‰ with zymotic fluid mass ratio, withered grass gemma
Bacillus species are 0.1~0.2 ‰ with zymotic fluid mass ratio.
Further, above-mentioned steps 2) in low temperature concentrate temperature be 30~40 DEG C.
Further, above-mentioned steps 2) in low temperature concentration using vacuum distillation, concentration after concentration is 15~20%.
Further, above-mentioned steps 3) in emulsion process carried out in high speed shear dispersion emulsifying machine.
The microcapsules of the present invention are formed by the way of spray drying, and ethyl cellulose itself is not soluble in water, as micro- glue
The main component of cyst wall material, adds a small amount of hydrophily wall material, can realize that microcapsules are not disintegrated integrally in water, on cyst wall gradually
Form the effect that aperture slowly discharges intracapsular microorganism;Casein-sodium and Tween 80 are as emulsifier, after clipped dispersion and emulsion,
Microcapsule wall material can be made to stablize package cenobium, avoid the occurrence of wall material largely situation of the aggregation without wrapping up cenobium;It is husky to moor Lip river
Nurse hydrophily is strong, it may have certain emulsification can dissolve and make to gradually form aperture, capsule on effect microcapsule wall in water
Interior microorganism is released slowly by aperture in water;Gelatin can improve the intensity of effect microcapsule wall, reduce rupture.
Compared with prior art, beneficial effects of the present invention:
(1) the compound bacteria microcapsule water purification agent of this slow-release provided by the invention can slowly release the micro- life of water purification in water
Object plays lasting water purification effect, and forms composite bacteria agent using the strain of four kinds of different catharsis, can between a variety of strains
Mutually synergistic effect improves purification efficiency.
(2) the compound bacteria microcapsule water purification agent of this slow-release provided by the invention uses high molecular material on compound bacteria surface
A microcapsule protective film is formed, composite bacteria agent and external environment can be kept apart, extends the storage time of composite bacteria agent, and
Improve its bacterial activity.
(3) preparation method of the compound bacteria microcapsule water purification agent of this slow-release provided by the invention will by microcapsules technology
Composite bacteria agent wraps up, and preparation process is simple.
Specific embodiment
The technical solution in the embodiment of the present invention is clearly and completely described below, it is clear that described embodiment
Only part of the embodiment of the present invention, instead of all the embodiments.Based on the embodiments of the present invention, the common skill in this field
All other embodiment that art personnel are obtained without making creative work belongs to the model that the present invention protects
It encloses.
Embodiment 1:
A kind of preparation method of the compound bacteria microcapsule water purification agent of slow-release is present embodiments provided, is included the following steps:
1) 0.5g polyP bacterias, 0.3g nitrobacterias, 0.1g photosynthetic bacterias, 0.1g bacillus subtilis are added in 1000g zymotic fluids
Bacterium is fermented;
2) after the completion of step 1) fermentation, the solids in zymotic fluid is filtered out, and using vacuum distillation at a temperature of 30 DEG C
Mode carries out low temperature concentration to zymotic fluid, and the concentration after concentration is 15%;
3) in zymotic fluid after concentration, add in and ethyl cellulose of the weight ratio of zymotic fluid for 21 ‰, the weight with zymotic fluid
The Tween 80 and the weight ratio of zymotic fluid that the casein-sodium and zymotic fluid weight ratio that amount ratio is 0.8 ‰ are 0.9 ‰ are 0.5 ‰
Poloxamer, the gelatin that the weight ratio with zymotic fluid is 0.2 ‰ spread mulser using high speed shear point and are emulsified, finally sprayed
Compound bacteria microcapsule water purification agent is made in mist drying.
Embodiment 2:
A kind of preparation method of the compound bacteria microcapsule water purification agent of slow-release is present embodiments provided, is included the following steps:
1) 0.6g polyP bacterias, 0.35g nitrobacterias, 0.12g photosynthetic bacterias, 0.15g withered grass gemma are added in 1000g zymotic fluids
Bacillus is fermented;
2) after the completion of step 1) fermentation, the solids in zymotic fluid is filtered out, and using vacuum distillation at a temperature of 35 DEG C
Mode carries out low temperature concentration to zymotic fluid, and the concentration after concentration is 17%;
3) in zymotic fluid after concentration, add in and ethyl cellulose of the weight ratio of zymotic fluid for 23 ‰, the weight with zymotic fluid
The Tween 80 and the weight ratio of zymotic fluid that the casein-sodium and zymotic fluid weight ratio that amount ratio is 1.0 ‰ are 1.0 ‰ are 0.6 ‰
Poloxamer, the gelatin that the weight ratio with zymotic fluid is 0.3 ‰ spread mulser using high speed shear point and are emulsified, finally sprayed
Compound bacteria microcapsule water purification agent is made in mist drying.
Embodiment 3:
A kind of preparation method of the compound bacteria microcapsule water purification agent of slow-release is present embodiments provided, is included the following steps:
1) 0.7g polyP bacterias, 0.4g nitrobacterias, 0.15g photosynthetic bacterias, 0.2g bacillus subtilis are added in 1000g zymotic fluids
Bacterium is fermented;
2) after the completion of step 1) fermentation, the solids in zymotic fluid is filtered out, and using vacuum distillation at a temperature of 40 DEG C
Mode carries out low temperature concentration to zymotic fluid, and the concentration after concentration is 20%;
3) in zymotic fluid after concentration, add in and ethyl cellulose of the weight ratio of zymotic fluid for 25 ‰, the weight with zymotic fluid
The Tween 80 and the weight ratio of zymotic fluid that the casein-sodium and zymotic fluid weight ratio that amount ratio is 1.2 ‰ are 1.3 ‰ are 1.0 ‰
The weight ratio of poloxamer and zymotic fluid is 0.5 ‰ gelatin, and spreading mulser using high speed shear point is emulsified, and is finally sprayed
Compound bacteria microcapsule water purification agent is made in mist drying.
The present invention also provides a kind of compound bacteria microcapsule water purification agents of slow-release, are made of above-mentioned preparation method,
Purifying water micrcxDrganism can be slowly released in water, plays lasting water purification effect, and using the strain of four kinds of different catharsis
Composite bacteria agent is formed, can mutually be acted synergistically between a variety of strains, improves purification efficiency.
The foregoing examples are only illustrative of the present invention, does not form the limitation to protection scope of the present invention, all
Be with the present invention it is the same or similar design all belong to the scope of protection of the present invention within.
Claims (8)
1. a kind of compound bacteria microcapsule water purification agent of slow-release, it is characterised in that:Zymotic fluid including microcapsules and compound bacteria, it is described
Compound bacteria is polyP bacteria, nitrobacteria, photosynthetic bacteria and bacillus subtilis, and the microcapsules are included by concentrated broth weight
The following component of percentages:21 ‰~25 ‰ ethyl cellulose, 0.8 ‰~1.2 ‰ casein-sodium, 0.9 ‰~1.3 ‰
Tween 80,0.5 ‰~1.0 ‰ poloxamer, 0.2 ‰~0.5 ‰ gelatin.
2. the compound bacteria microcapsule water purification agent of slow-release as described in claim 1, it is characterised in that:The microcapsules are included by dense
The following component of contracting zymotic fluid weight percent meter:23 ‰~25 ‰ ethyl cellulose, 1.0 ‰~1.2 ‰ casein-sodium,
1.1 ‰~1.3 ‰ Tween 80,0.8 ‰~1.0 ‰ poloxamer, 0.4 ‰~0.5 ‰ gelatin.
3. the compound bacteria microcapsule water purification agent of slow-release as described in claim 1, it is characterised in that:The microcapsules are included by dense
The following component of contracting zymotic fluid weight percent meter:25 ‰ ethyl cellulose, 1.2 ‰ casein-sodium, 1.3 ‰ tween
80th, 1.0 ‰ poloxamer, 0.5 ‰ gelatin.
It is 4. a kind of such as the preparation method of any one of the claims 1 to 3 compound bacteria microcapsule water purification agent of slow-release, it is characterised in that:
Include the following steps:1) polyP bacteria, nitrobacteria, photosynthetic bacteria, bacillus subtilis is added in zymotic fluid to ferment;
2) solids in zymotic fluid is filtered out after the completion of step 1) fermentation, low temperature concentration is carried out to zymotic fluid;
3) in zymotic fluid after concentration, ethyl cellulose, casein-sodium, Tween 80, poloxamer, gelatin are added in, stirring is molten
The compound bacteria microcapsule water purification agent of slow-release is made through emulsifying, being spray-dried after solution is uniform.
5. the preparation method of the compound bacteria microcapsule water purification agent of slow-release as claimed in claim 4, it is characterised in that:The step
1) polyP bacteria strain and zymotic fluid mass ratio are 0.5~0.7 ‰ in, nitrobacteria strain and zymotic fluid mass ratio for 0.3~
0.4 ‰, photosynthetic bacteria strain is 0.1~0.2 ‰ with zymotic fluid mass ratio, and Bacillus subtilis strain is with zymotic fluid mass ratio
0.1~0.2 ‰.
6. the preparation method of the compound bacteria microcapsule water purification agent of slow-release as claimed in claim 4, it is characterised in that:The step
2) temperature that low temperature concentrates in is 30~40 DEG C.
7. the preparation method of the compound bacteria microcapsule water purification agent of slow-release as claimed in claim 4, it is characterised in that:The step
2) using vacuum distillation, the concentration after concentration is 15~20% for low temperature concentration in.
8. the preparation method of the compound bacteria microcapsule water purification agent of slow-release as claimed in claim 4, it is characterised in that:The step
3) emulsion process carries out in high speed shear dispersion emulsifying machine in.
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Cited By (2)
Publication number | Priority date | Publication date | Assignee | Title |
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CN109607821A (en) * | 2018-12-25 | 2019-04-12 | 武汉沃田生态科技有限公司 | A kind of shelf-stable microorganism dephosphorization agent and preparation method thereof |
CN109775866A (en) * | 2019-03-13 | 2019-05-21 | 南京工业大学 | Handle the preparation method of the composite slow release microcapsule formulation of breeding water body ammonia nitrogen |
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CN101015537A (en) * | 2007-02-15 | 2007-08-15 | 河北理工大学 | Preparation method of sodium iron chlorophyllin-ethyl cellulose microcapsule |
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Application publication date: 20180529 |