CN106348457A - Method for self diffusion composite bacteria microcapsule water purifying agent and preparing method thereof - Google Patents
Method for self diffusion composite bacteria microcapsule water purifying agent and preparing method thereof Download PDFInfo
- Publication number
- CN106348457A CN106348457A CN201610762986.5A CN201610762986A CN106348457A CN 106348457 A CN106348457 A CN 106348457A CN 201610762986 A CN201610762986 A CN 201610762986A CN 106348457 A CN106348457 A CN 106348457A
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- fermentation liquid
- microcapsule
- bacteria
- water purification
- purification agent
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
Abstract
The invention provides a self diffusion composite bacteria microcapsule water purifying agent, including fermentation broth for microcapsules and composite bacteria, the composite bacteria is nitrifying bacteria, photosynthetic bacteria and bacillus subtilis, the microcapsules comprise the ingredients, by weight: 1.5 %o -2.5 %o of xanthan gum, 0.8 %o-1.2 %o of casein sodium, 0.9 %o -1.3 %o of gum arabic, 3 %o -5 %o Tween 80,8 %o -11 %o of poloxamer, 0.2 %o -0.5 %o of gelatin, 1 %o -1.5 %o of starch. The composite bacteria microcapsule water purifying agent is prepared by covering the composite bacteria with microcapsule so as to separate the composite bacteria from outside and improve bacteria activity, meanwhile, the composite microcapsule water purifying agent can dissolve in the water quickly, and distribute evenly, the multiple strains of bacteria can coordinate with each other so as to improve the purifying effect.
Description
Technical field
The invention belongs to ecological, environmental protective technical field is and in particular to a kind of compound bacteria microcapsule water purification agent that can voluntarily spread
And preparation method thereof, for purifying to water body.
Background technology
Nitrobacteria (nitrifying bacteria) is a kind of aerobic bacteria, including Nitrosomas and Nitromonas, nitre
The circulation conversion changing antibacterial for nitrogen in nature has great importance, and organic ammonia nitrogen can be oxidized to cultured water or nitric acid
Nitrogen, is then removed by Denitrification, and this plays an important role to the removal of Water pollution.Photosynthetic bacteria
(photosynthetic bacteria) be occur on the earth earliest, generally existing in nature, there is original luminous energy compound body
The prokaryote of system, is the general name not put the photosynthetic antibacterial of oxygen under anaerobic, is that a class does not form spore
The gram negative bacteria of ability, is using light as the energy, can utilize in nature under anaerobism illumination or aerobic dark condition
Organic substance, sulfide, ammonia etc. carry out photosynthetic microorganism as hydrogen donor and carbon source；After photosynthetic bacteria applies water body, it
Organic particle in degradable water body is moreover it is possible to absorb the harmful substances such as the ammonia in water body, nitrite, hydrogen sulfide.Withered
Careless bacilluss (bacillus subtilis), are one kind of Bacilluss, 0.7～0.8 × 2～3 microns of individual cells,
Uniform coloring, no pod membrane, peritrichouses, can move, gram positive bacteria, 0.6～0.9 × 1.0～1.5 microns of spore, oval
To column, central or slightly inclined positioned at thalline, after sporulation, thalline does not expand；Bacillus subtilises have in very strong cleaning water
The short grained effect of rubbish, has very strong suppression to make to harmful microorganisms such as the vibrio in Aquatic product, escherichia coli and baculoviruss
With the water turbidity problem causing additionally it is possible to the harmful cyanophyceae of improvement is overflowed.These three microbial association uses can have been given play to more
Big purifying water effect.
Currently, domestic water pollution is increasingly severe, using the technology attention of biological cleaning water body, particularly micro- life
Thing water purification microbial inoculum.However, traditional water purification microbial inoculum is all addition excipient in ferment product, after mix homogeneously, make powdery
Or graininess microbial inoculum, this microbial inoculum is easily mutually polymerized agglomerating, diffusion difficulty after putting in water, so that skewness in water body
Even it is difficult to give full play to clean-up effect.
Content of the invention
The purpose of the present invention be overcome existing water purification microbial inoculum to put in water after easily mutually polymerization agglomerating, diffusion is difficult, only
Change the problem of effect difference.
For this reason, the invention provides a kind of compound bacteria microcapsule water purification agent that can voluntarily spread, including microcapsule and compound
The fermentation liquid of bacterium, described compound bacteria is nitrobacteria, photosynthetic bacteria and bacillus subtilis, and described microcapsule is included by fermentation liquid weight
Amount percentages following component: 1.5 ‰～2.5 ‰ xanthan gum, 0.8 ‰～1.2 ‰ casein sidium, 0.9 ‰～1.3 ‰
Radix Acaciae senegalis, 3 ‰～5 ‰ Tween 80,8 ‰～11 ‰ poloxamer, 0.2 ‰～0.5 ‰ gelatin, 1 ‰～
1.5 ‰ starch.
Further, above-mentioned microcapsule includes the following component based on fermentation liquid percentage by weight: 1.5 ‰～1.7 ‰
Xanthan gum, 0.8 ‰～0.9 ‰ casein sidium, 0.9 ‰～1.0 ‰ Radix Acaciae senegalis, 3 ‰～3.5 ‰ Tween 80,8 ‰
～9 ‰ poloxamer, 0.4 ‰～0.5 ‰ gelatin, 1 ‰～1.1 ‰ starch.
Further, above-mentioned microcapsule includes the following component based on fermentation liquid percentage by weight: 1.7 ‰ xanthan gum,
0.9 ‰ casein sidium, 1.0 ‰ Radix Acaciae senegalis, 3.5 ‰ Tween 80,9 ‰ poloxamer, 0.4 ‰ gelatin,
1.1 ‰ starch.
In addition, present invention also offers a kind of preparation method of the compound bacteria microcapsule water purification agent that can voluntarily spread, including
Following steps:
1) nitrobacteria, photosynthetic bacteria, bacillus subtilises are added to be fermented in fermentation liquid.
2) step 1) filter out solidss in fermentation liquid after the completion of fermentation, cryoconcentration is carried out to fermentation liquid.
3) in fermentation liquid after concentration, addition xanthan gum, casein sidium, Radix Acaciae senegalis, Tween 80, poloxamer,
Gelatin and starch, make compound bacteria microcapsule water purification agent through emulsifying, spray drying after stirring and dissolving is uniform.
Further, above-mentioned steps 1) in nitrobacteria strain and fermentation liquid mass ratio be 0.2～0.25 ‰, photosynthetic bacteria
Strain and fermentation liquid mass ratio are 0.1～0.15 ‰, and Bacillus subtilis strain and fermentation liquid mass ratio are 0.3～0.4 ‰.
Further, above-mentioned steps 2) in cryoconcentration temperature be 40～50 DEG C.
Further, above-mentioned steps 2) in cryoconcentration adopt vacuum distillation, the concentration after concentration be 10～20%.
Further, above-mentioned steps 3) in emulsion process carry out in high speed shear dispersion emulsifying machine.
The microcapsule of the present invention is formed by the way of being spray-dried, and poloxamer hydrophilic is strong, and it is as microcapsule wall
Material, can be with self-dispersing in water, and rate of dispersion is fast；Radix Acaciae senegalis, gelatin and starch assist wall material as microcapsule, can increase
The toughness of strong microcapsule wall and intensity, it is to avoid the easily rupturable problem of the simple microcapsule with poloxamer as wall material；Xanthan gum and
Casein sidium, as stabilizer, coemulsifier, promotes the formation of stable emulsion；Tween 80, as emulsifying agent, can make Huang
Virgin rubber stably wraps up cenobium, it is to avoid a large amount of situation about assembling of xanthan gum；Add xanthan gum, casein sidium and Tween 80, warp
After shearing dispersion and emulsion, microcapsule wall material stable and uniform can be made to be dispersed in around microorganism, can be formed multiple after spray drying
Close microbial inoculum microcapsule.
Compared with prior art, beneficial effects of the present invention:
(1) this compound bacteria microcapsule water purification agent that can voluntarily spread that the present invention provides can quickly voluntarily divide in water
Dissipate, and can more uniformly be distributed, and composite bacteria agent capable is constituted using the strain of three kinds of different purifications, between multiple strains
Can mutual synergism, improve purification efficiency.
(2) this compound bacteria microcapsule water purification agent that can voluntarily spread that the present invention provides adopts macromolecular material compound
Bacterium surface forms a microcapsule protecting film, can keep apart composite bacteria agent capable with external environment, extends the storage of composite bacteria agent capable
Time, and improve its bacterial activity.
(3) preparation method of this compound bacteria microcapsule water purification agent that can voluntarily spread that the present invention provides passes through microcapsule
Composite bacteria agent capable is wrapped up by technology, and preparation process is simple.
Specific embodiment
Below the embodiment it is clear that described is clearly and completely described to the technical scheme in the embodiment of the present invention
It is only a part of embodiment of the present invention, rather than whole embodiments.Based on the embodiment in the present invention, the common skill in this area
All other embodiment that art personnel are obtained under the premise of not making creative work, broadly falls into the model of present invention protection
Enclose.
Embodiment 1:
Present embodiments provide a kind of preparation method of the compound bacteria microcapsule water purification agent that can voluntarily spread, walk including following
Rapid:
1) 0.2g nitrobacteria, 0.1g photosynthetic bacteria, 0.3g bacillus subtilises are added to carry out sending out in 1000g fermentation liquid
Ferment.
2) step 1) fermentation after the completion of, filter out the solidss in fermentation liquid, and at a temperature of 40 DEG C using decompression steam
The mode evaporating carries out cryoconcentration to fermentation liquid, and the concentration after concentration is 10%.
3), in fermentation liquid after concentration, add the weight than the xanthan gum for 1.5 ‰ and fermentation liquid with the weight of fermentation liquid
Amount than the casein sidium for 0.8 ‰, with fermentation liquid weight than the Radix Acaciae senegalis for 0.9 ‰, with the weight of fermentation liquid ratio for 3 ‰
Tween 80, with the weight of fermentation liquid than the poloxamer for 8 ‰, with the weight of fermentation liquid than the gelatin for 0.2 ‰ and fermentation
Liquid weight, than the starch for 1 ‰, is divided using high speed shear and is spread mulser and carry out emulsifying, is finally spray-dried and makes the micro- glue of compound bacteria
Capsule water purification agent.
Embodiment 2:
Present embodiments provide a kind of preparation method of the compound bacteria microcapsule water purification agent that can voluntarily spread, walk including following
Rapid:
1) 0.25g nitrobacteria, 0.15g photosynthetic bacteria, 0.4g bacillus subtilises are added to carry out in 1000g fermentation liquid
Fermentation.
2) step 1) fermentation after the completion of, filter out the solidss in fermentation liquid, and at a temperature of 55 DEG C using decompression steam
The mode evaporating carries out cryoconcentration to fermentation liquid, and the concentration after concentration is 20%.
3), in fermentation liquid after concentration, add the weight than the xanthan gum for 2.5 ‰ and fermentation liquid with the weight of fermentation liquid
Amount than the casein sidium for 1.2 ‰, with fermentation liquid weight than the Radix Acaciae senegalis for 1.3 ‰, with the weight of fermentation liquid ratio for 5 ‰
Tween 80, with the weight of fermentation liquid than the poloxamer for 11 ‰, and send out than the gelatin for 0.5 ‰ with the weight of fermentation liquid
Zymotic fluid weight, than the starch for 1.5 ‰, is divided using high speed shear and is spread mulser and carry out emulsifying, is finally spray-dried and makes compound bacteria
Microcapsule water purification agent.
Embodiment 3:
Present embodiments provide a kind of preparation method of the compound bacteria microcapsule water purification agent that can voluntarily spread, walk including following
Rapid:
1) 0.22g nitrobacteria, 0.13g photosynthetic bacteria, 0.35g bacillus subtilises are added to enter in 1000g fermentation liquid
Row fermentation.
2) step 1) fermentation after the completion of, filter out the solidss in fermentation liquid, and at a temperature of 45 DEG C using decompression steam
The mode evaporating carries out cryoconcentration to fermentation liquid, and the concentration after concentration is 15%.
3), in fermentation liquid after concentration, add the weight than the xanthan gum for 1.7 ‰ and fermentation liquid with the weight of fermentation liquid
Amount than the casein sidium for 0.9 ‰, with fermentation liquid weight than the Radix Acaciae senegalis for 1.0 ‰, with the weight ratio of fermentation liquid be
3.5 ‰ Tween 80, with the weight of fermentation liquid than the poloxamer for 9 ‰, with the weight of fermentation liquid than the gelatin for 0.4 ‰,
With fermentation liquid weight than the starch for 1.1 ‰, divided using high speed shear and spread mulser and carry out emulsifying, be finally spray-dried make multiple
Close bacteria microcapsule water purification agent.
Present invention also offers a kind of compound bacteria microcapsule water purification agent voluntarily spreading, made using above-mentioned preparation method,
Its storage time is long, in water can quick self-dispersing, and more can uniformly be distributed, and be made using three kinds of different purifications
Strain constitutes composite bacteria agent capable, between multiple strains can mutual synergism, raising purification efficiency.
The only illustration to the present invention exemplified as above, does not constitute the restriction to protection scope of the present invention, all
It is that same or analogous design belongs within protection scope of the present invention with the present invention.
Claims (8)
1. a kind of compound bacteria microcapsule water purification agent that can voluntarily spread it is characterised in that: include the fermentation of microcapsule and compound bacteria
Liquid, described compound bacteria is nitrobacteria, photosynthetic bacteria and bacillus subtilis, and described microcapsule is included by fermentation liquid percentage by weight
The following component of meter: 1.5 ‰～2.5 ‰ xanthan gum, 0.8 ‰～1.2 ‰ casein sidium, 0.9 ‰～1.3 ‰ Arab
Natural gum, 3 ‰～5 ‰ Tween 80,8 ‰～11 ‰ poloxamer, 0.2 ‰～0.5 ‰ gelatin, 1 ‰～1.5 ‰ shallow lake
Powder.
2. the compound bacteria microcapsule water purification agent that can voluntarily spread as claimed in claim 1 it is characterised in that: described microcapsule bag
Include the following component based on fermentation liquid percentage by weight: 1.5 ‰～1.7 ‰ xanthan gum, 0.8 ‰～0.9 ‰ casein sidium,
0.9 ‰～1.0 ‰ Radix Acaciae senegalis, 3 ‰～3.5 ‰ Tween 80,8 ‰～9 ‰ poloxamer, 0.4 ‰～0.5 ‰
Gelatin, 1 ‰～1.1 ‰ starch.
3. the compound bacteria microcapsule water purification agent that can voluntarily spread as claimed in claim 1 it is characterised in that: described microcapsule bag
Include the following component based on fermentation liquid percentage by weight: 1.7 ‰ xanthan gum, 0.9 ‰ casein sidium, 1.0 ‰ Arab
Natural gum, 3.5 ‰ Tween 80,9 ‰ poloxamer, 0.4 ‰ gelatin, 1.1 ‰ starch.
4. a kind of preparation method of the such as compound bacteria microcapsule water purification agent that any one of claims 1 to 3 can voluntarily spread, its feature
It is: comprise the steps:
1) nitrobacteria, photosynthetic bacteria, bacillus subtilises are added to be fermented in fermentation liquid；
2) step 1) filter out solidss in fermentation liquid after the completion of fermentation, cryoconcentration is carried out to fermentation liquid；
3), in fermentation liquid after concentration, xanthan gum, casein sidium, Radix Acaciae senegalis, Tween 80, poloxamer, gelatin are added
And starch, make compound bacteria microcapsule water purification agent through emulsifying, spray drying after stirring and dissolving is uniform.
5. the preparation method of the compound bacteria microcapsule water purification agent that can voluntarily spread as claimed in claim 4 it is characterised in that: institute
State step 1) in nitrobacteria strain and fermentation liquid mass ratio be 0.2～0.25 ‰, photosynthetic bacteria strain and fermentation liquid mass ratio
For 0.1～0.15 ‰, Bacillus subtilis strain and fermentation liquid mass ratio are 0.3～0.4 ‰.
6. the preparation method of the compound bacteria microcapsule water purification agent that can voluntarily spread as claimed in claim 4 it is characterised in that: institute
State step 2) in cryoconcentration temperature be 40～50 DEG C.
7. the preparation method of the compound bacteria microcapsule water purification agent that can voluntarily spread as claimed in claim 4 it is characterised in that: institute
State step 2) in cryoconcentration adopt vacuum distillation, the concentration after concentration be 10～20%.
8. the preparation method of the compound bacteria microcapsule water purification agent that can voluntarily spread as claimed in claim 4 it is characterised in that: institute
State step 3) in emulsion process carry out in high speed shear dispersion emulsifying machine.
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| CN201610762986.5A CN106348457B (en) | 2016-08-30 | 2016-08-30 | A kind of compound bacteria microcapsule water purification agent and preparation method thereof that can voluntarily spread |
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Cited By (2)
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| CN108083451A (en) * | 2017-12-12 | 2018-05-29 | 武汉沃田生态科技有限公司 | A kind of compound bacteria microcapsule water purification agent of slow-release and preparation method thereof |
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Cited By (2)
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| CN107098425A (en) * | 2017-05-05 | 2017-08-29 | 中科众沃生态科技（武汉）有限公司 | A kind of microcapsules water purification agent and preparation method thereof |
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| CN106348457B (en) | 2019-11-15 |
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