CN108079372A - A kind of preparation method of dermal tissue repair materials - Google Patents
A kind of preparation method of dermal tissue repair materials Download PDFInfo
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- CN108079372A CN108079372A CN201810074599.1A CN201810074599A CN108079372A CN 108079372 A CN108079372 A CN 108079372A CN 201810074599 A CN201810074599 A CN 201810074599A CN 108079372 A CN108079372 A CN 108079372A
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- dermal tissue
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Abstract
The invention discloses a kind of dermal tissue repair materials and preparation method thereof, are mainly made of following raw material by mass fraction proportioning：Keratin 13~32 part, 15~27 parts of eleidin, 12~26 parts of collagen, 11~28 parts of collagenous fibres, 8~30 parts of elastic fibers, 9~18 parts of reticular fibre, 7~24 parts of aminoglucan, 6~22 parts of hyaluronic acid, 6~27 parts of chitosan, 1~7 part of Fibronectin, 35~60 parts of deionized water.The present invention has the following advantages：First, the dermal tissue repair materials and the dermal tissue of people that prepared by scheme of the present invention have height amalgamation, and repairing efficiency is short；Secondly, the dermal tissue repair materials that prepared by scheme of the present invention use simplicity, and without any side effects, and safety is without healthy hidden danger.
Description
The application is Application No. 201510352562.7, the applying date：It is on 06 24th, 2015, entitled " a kind of
The divisional application of the patent of invention of dermal tissue repair materials and preparation method thereof ".
Technical field
The present invention relates to biomaterial for medical purpose fields more particularly to a kind of dermal tissue repair materials and preparation method thereof.
Background technology
Skin is made of epidermis, corium and subcutaneous tissue, and containing accessory organ's (sweat gland, sebaceous glands, nail, toenail) with
And blood vessel, lymphatic vessel, nerves and muscles etc..Skin is the organ of human body area maximum.The skin developed area of one adult
At 2 square metres or so, weight is about the 1/20 of human body weight.Most thick skin is in foot bottom, and thickness is up to 4 millimeters, on eyelid
Skin is most thin, only less than 1 millimeter.
Corium is located at epidermis deep layer, is connected with subcutaneous tissue downwards, with the latter without apparent boundary.Corium is by fine and close connective group
Knit composition.Various phoirocytes and substantial amounts of collagenous fibres elastomer are dispersed in it, makes skin not only flexible, but also has
Toughness.Phoirocyte is more with fibroblast and mast cell.Corium between epidermis and subcutaneous tissue, mainly by
The connective tissues such as collagenous fibres, elastic fibers, reticular fibre and amorphous ground substance are formed, wherein also have nerve and nerve endings,
The appendicle of blood vessel, lymphatic vessel, muscle and skin.
Corium is thicker than epidermis, containing great number of elastic fiber and collagenous fibres, skin is made to have certain elasticity and toughness, corium
It is interior to contain abundant blood vessel and sensory nerve ending.The collagenous fibres and elastomer of dermal connective tissue are interleaved with each other one
It rises, is embedded in Medium Culture.Cell component has fibroblast, histocyte and mast cell etc. in normal dermal.Collagenous fibres, bullet
Property fiber and matrix be all by fibroblast secrete generate.Reticular fibre is inmature collagenous fibres, and not one is independent
Ingredient.The thickness of dermal tissue and the number of its fibr tissue and matrix are in close relations, and with the compactness of skin, plumpness,
Relaxation is closely related with wrinkling, is paid close attention in recent years be subject to more and more Skin cosmesis scholars.
The content of the invention
The technical issues of solution：In order to solve the deficiency that skin dermis repairing efficiency is long, difficulty is big in the prior art,
The present invention provides a kind of dermal tissue repair materials and preparation method thereof.
To achieve the above object, the present invention uses following technical scheme：
A kind of dermal tissue repair materials are mainly made of following raw material by mass fraction proportioning：Keratin 13~32 part,
15~27 parts of eleidin, 12~26 parts of collagen, 11~28 parts of collagenous fibres, 8~30 parts of elastic fibers, reticular fibre 9
~18 parts, 7~24 parts of aminoglucan, 6~22 parts of hyaluronic acid, 6~27 parts of chitosan, 1~7 part of Fibronectin, go from
Sub- 35~60 parts of water.
As a preferred embodiment of the present invention, the dermal tissue repair materials mainly press mass fraction by following raw material
Proportioning is made：It is 24 parts of keratin, 21 parts of eleidin, 19 parts of collagen, 22 parts of collagenous fibres, 18 parts of elastic fibers, netted
12 parts of fiber, 22 parts of aminoglucan, 15 parts of hyaluronic acid, 18 parts of chitosan, 5 parts of Fibronectin, 50 parts of deionized water.
A kind of preparation method of dermal tissue repair materials, comprises the steps of：
(1) keratin, eleidin and collagen are dissolved in deionized water, are heated to 22~32 DEG C and slurry 1 is made；
Slurry 1 is deposited in a mold, cools and is converted into multiple crystal or particle to slurry 1；
(2) crystal or particle that are obtained in step (1) are heated to 61~82 DEG C, composite porous 1 are obtained, from mold
Middle taking-up material；
(3) collagenous fibres, elastic fibers, reticular fibre and hyaluronic acid are dissolved in deionized water, are heated to 52~72
DEG C slurry 2 is made；After slurry 2 is deposited in mold, -22~-3 DEG C are cooled to, until slurry 2 is completely converted into multiple crystal
Or after particle, 37~61 DEG C are warming up to the speed of 6~14 DEG C/s, composite porous 2 is obtained, is removed from the molds material；
(4) 102~134 DEG C are heated to by aminoglucan, chitosan and, Fibronectin is added in it after melting completely
In and stir evenly, be applied to composite porous 1 and/or composite porous 2 one side, and the face is staggered relatively, from
And by composite porous 1 and composite porous 2 adhesion-molded, dermal tissue repair materials are made.
As a preferred technical solution of the present invention, keratin, eleidin and collagen are dissolved in by step in (1)
In deionized water, it is heated to 28 DEG C and slurry 1 is made；Slurry 1 is deposited in a mold, cooling to slurry 1, it is multiple to be converted into
Crystal or particle.
As a preferred technical solution of the present invention, the crystal or particle that are obtained in step (1) are heated in step (2)
To 76 DEG C, composite porous 1 is obtained, is removed from the molds material.
As a preferred technical solution of the present invention, in step (3) by collagenous fibres, elastic fibers, reticular fibre and
Hyaluronic acid is dissolved in deionized water, is heated to 67 DEG C and slurry 2 is made；After slurry 2 is deposited in mold, -17 are cooled to
DEG C, until after slurry 2 is completely converted into multiple crystal or particle, 58 DEG C are warming up to the speed of 12 DEG C/s, obtains porous composite wood
Material 2, is removed from the molds material.
By aminoglucan, chitosan and 128 are heated to as a preferred technical solution of the present invention, in step (4)
DEG C, Fibronectin is added thereto and stirred evenly after melting completely, is applied to composite porous 1 and/or porous compound
The one side of material 2, and the face is staggered relatively, so as to by composite porous 1 and composite porous 2 adhesion-molded, be made
Dermal tissue repair materials.
Advantageous effect
Compared with prior art, the invention has the advantages that：
First, the dermal tissue repair materials that prepared by scheme of the present invention with the dermal tissue of people there is height to merge
Property, and repairing efficiency is short；
Secondly, the dermal tissue repair materials that prepared by scheme of the present invention use easy and without any side effects, peace
Completely without healthy hidden danger.
Specific embodiment
Embodiment 1：
A kind of dermal tissue repair materials are mainly made of following raw material by mass fraction proportioning：Keratin 13 part, angle are female
It is protein 15 part, 12 parts of collagen, 11 parts of collagenous fibres, 8 parts of elastic fibers, 9 parts of reticular fibre, 7 parts of aminoglucan, transparent
6 parts of matter acid, 6 parts of chitosan, 1 part of Fibronectin, 35 parts of deionized water.
A kind of preparation method of dermal tissue repair materials, comprises the steps of：
(1) keratin, eleidin and collagen are dissolved in deionized water, are heated to 22 DEG C and slurry 1 is made；It will slurry
1 deposition of material in a mold, cools and is converted into multiple crystal or particle to slurry 1；
(2) crystal or particle that are obtained in step (1) are heated to 61 DEG C, obtain composite porous 1, taken from mold
Go out material；
(3) collagenous fibres, elastic fibers, reticular fibre and hyaluronic acid are dissolved in deionized water, are heated to 52 DEG C of systems
Into slurry 2；After slurry 2 is deposited in mold, -3 DEG C are cooled to, until after slurry 2 is completely converted into multiple crystal or particle,
37 DEG C are warming up to the speed of 6 DEG C/s, composite porous 2 is obtained, is removed from the molds material；
(4) 102 DEG C are heated to by aminoglucan, chitosan and, is completely added thereto Fibronectin simultaneously after melting
It stirs evenly, is applied to composite porous 1 and/or composite porous 2 one side, and the face is staggered relatively, thus will
Composite porous 1 and composite porous 2 adhesion-molded, dermal tissue repair materials are made.
Embodiment 2：
A kind of dermal tissue repair materials are mainly made of following raw material by mass fraction proportioning：24 parts of keratin, angle are female
Protein 21 part, 19 parts of collagen, 22 parts of collagenous fibres, 18 parts of elastic fibers, 12 parts of reticular fibre, 22 parts of aminoglucan,
15 parts of hyaluronic acid, 18 parts of chitosan, 5 parts of Fibronectin, 50 parts of deionized water.
A kind of preparation method of dermal tissue repair materials, comprises the steps of：
(1) keratin, eleidin and collagen are dissolved in deionized water, are heated to 28 DEG C and slurry 1 is made；It will slurry
1 deposition of material in a mold, cools and is converted into multiple crystal or particle to slurry 1；
(2) crystal or particle that are obtained in step (1) are heated to 76 DEG C, obtain composite porous 1, taken from mold
Go out material；
(3) collagenous fibres, elastic fibers, reticular fibre and hyaluronic acid are dissolved in deionized water, are heated to 67 DEG C of systems
Into slurry 2；After slurry 2 is deposited in mold, -17 DEG C are cooled to, until after slurry 2 is completely converted into multiple crystal or particle,
58 DEG C are warming up to the speed of 12 DEG C/s, composite porous 2 is obtained, is removed from the molds material；
(4) 128 DEG C are heated to by aminoglucan, chitosan and, is completely added thereto Fibronectin simultaneously after melting
It stirs evenly, is applied to composite porous 1 and/or composite porous 2 one side, and the face is staggered relatively, thus will
Composite porous 1 and composite porous 2 adhesion-molded, dermal tissue repair materials are made.
Embodiment 3：
A kind of dermal tissue repair materials are mainly made of following raw material by mass fraction proportioning：32 parts of keratin, angle are female
27 parts of albumen, 26 parts of collagen, 28 parts of collagenous fibres, 30 parts of elastic fibers, 18 parts of reticular fibre, 24 parts of aminoglucan,
22 parts of hyaluronic acid, 27 parts of chitosan, 7 parts of Fibronectin, 60 parts of deionized water.
A kind of preparation method of dermal tissue repair materials, comprises the steps of：
(1) keratin, eleidin and collagen are dissolved in deionized water, are heated to 32 DEG C and slurry 1 is made；It will slurry
1 deposition of material in a mold, cools and is converted into multiple crystal or particle to slurry 1；
(2) crystal or particle that are obtained in step (1) are heated to 82 DEG C, obtain composite porous 1, taken from mold
Go out material；
(3) collagenous fibres, elastic fibers, reticular fibre and hyaluronic acid are dissolved in deionized water, are heated to 72 DEG C of systems
Into slurry 2；After slurry 2 is deposited in mold, -22 DEG C are cooled to, until after slurry 2 is completely converted into multiple crystal or particle,
61 DEG C are warming up to the speed of 14 DEG C/s, composite porous 2 is obtained, is removed from the molds material；
(4) 134 DEG C are heated to by aminoglucan, chitosan and, is completely added thereto Fibronectin simultaneously after melting
It stirs evenly, is applied to composite porous 1 and/or composite porous 2 one side, and the face is staggered relatively, thus will
Composite porous 1 and composite porous 2 adhesion-molded, dermal tissue repair materials are made.
The dermal tissue repair materials that Examples 1 to 3 is prepared are detected, the results are shown in table below：
| PH value | Water content/% | Repairing efficiency | Situation is merged with human body dermal tissue | |
| Embodiment 1 | 6.5 | 32 | 8 days | Preferably |
| Embodiment 2 | 7 | 34 | 4 days | Fusion completely |
| Embodiment 3 | 7 | 36 | 6 days | Fusion completely |
Claims (6)
1. a kind of preparation method of dermal tissue repair materials, which is characterized in that comprise the steps of：
(1) keratin, eleidin and collagen are dissolved in deionized water, are heated to 22~32 DEG C and slurry 1 is made；It will slurry
1 deposition of material in a mold, cools and is converted into multiple crystal or particle to slurry 1；
(2) crystal or particle that are obtained in step (1) are heated to 61~82 DEG C, obtain composite porous 1, taken from mold
Go out material；
(3) collagenous fibres, elastic fibers, reticular fibre and hyaluronic acid are dissolved in deionized water, are heated to 52~72 DEG C of systems
Into slurry 2；After slurry 2 is deposited in mold, be cooled to -22~-3 DEG C, until slurry 2 be completely converted into multiple crystal or
After grain, 37~61 DEG C are warming up to the speed of 6~14 DEG C/s, composite porous 2 is obtained, is removed from the molds material；
(4) 102~134 DEG C are heated to by aminoglucan, chitosan and, is completely added thereto Fibronectin simultaneously after melting
It stirs evenly, is applied to composite porous 1 and/or composite porous 2 one side, and the face is staggered relatively, thus will
Composite porous 1 and composite porous 2 adhesion-molded, dermal tissue repair materials are made.
A kind of 2. preparation method of dermal tissue repair materials according to claim 1, which is characterized in that step (1)~(5)
In raw material be according to the proportioning of mass fraction：Keratin 13~32 part, 15~27 parts of eleidin, collagen 12~26
Part, 11~28 parts of collagenous fibres, 8~30 parts of elastic fibers, 9~18 parts of reticular fibre, 7~24 parts of aminoglucan, hyalomitome
6~22 parts of acid, 6~27 parts of chitosan, 1~7 part of Fibronectin, 35~60 parts of deionized water.
3. a kind of preparation method of dermal tissue repair materials according to claim 1, which is characterized in that will in step (1)
Keratin, eleidin and collagen are dissolved in deionized water, are heated to 28 DEG C and slurry 1 is made；Slurry 1 is deposited on mold
In, it cools and is converted into multiple crystal or particle to slurry 1.
4. a kind of preparation method of dermal tissue repair materials according to claim 1, which is characterized in that will in step (2)
The crystal or particle obtained in step (1) is heated to 76 DEG C, obtains composite porous 1, is removed from the molds material.
5. a kind of preparation method of dermal tissue repair materials according to claim 1, which is characterized in that will in step (3)
Collagenous fibres, elastic fibers, reticular fibre and hyaluronic acid are dissolved in deionized water, are heated to 67 DEG C and slurry 2 is made；Treat slurry
After 2 are deposited in mold, -17 DEG C are cooled to, until after slurry 2 is completely converted into multiple crystal or particle, with the speed of 12 DEG C/s
58 DEG C are warming up to, composite porous 2 is obtained, is removed from the molds material.
6. a kind of preparation method of dermal tissue repair materials according to claim 1, which is characterized in that will in step (4)
It aminoglucan, chitosan and is heated to 128 DEG C, Fibronectin is added thereto and stirred evenly after melting completely, is smeared
It is and the face is staggered relatively to composite porous 1 and/or composite porous 2 one side, so as to by composite porous 1
It is adhesion-molded with composite porous 2, dermal tissue repair materials are made.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810074599.1A CN108079372A (en) | 2015-06-24 | 2015-06-24 | A kind of preparation method of dermal tissue repair materials |
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510352562.7A CN104984390B (en) | 2015-06-24 | 2015-06-24 | A kind of dermal tissue repair materials and preparation method thereof |
| CN201810074599.1A CN108079372A (en) | 2015-06-24 | 2015-06-24 | A kind of preparation method of dermal tissue repair materials |
Related Parent Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201510352562.7A Division CN104984390B (en) | 2015-06-24 | 2015-06-24 | A kind of dermal tissue repair materials and preparation method thereof |
Publications (1)
| Publication Number | Publication Date |
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| CN108079372A true CN108079372A (en) | 2018-05-29 |
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| CN201810074599.1A Withdrawn CN108079372A (en) | 2015-06-24 | 2015-06-24 | A kind of preparation method of dermal tissue repair materials |
| CN201510352562.7A Active CN104984390B (en) | 2015-06-24 | 2015-06-24 | A kind of dermal tissue repair materials and preparation method thereof |
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| Application Number | Title | Priority Date | Filing Date |
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| CN201510352562.7A Active CN104984390B (en) | 2015-06-24 | 2015-06-24 | A kind of dermal tissue repair materials and preparation method thereof |
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| CN109328058A (en) | 2016-07-27 | 2019-02-12 | 株式会社利他药业 | Oral mucosa patch material and its manufacturing method |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1255843A (en) * | 1997-05-13 | 2000-06-07 | 韦尔豪泽公司 | Reticulated absorbent composite |
| CN101507834A (en) * | 2008-07-14 | 2009-08-19 | 昆明理工大学 | Degradable skin tissue engineering bracket material and preparation method thereof |
| WO2010082138A2 (en) * | 2009-01-16 | 2010-07-22 | Geistlich Pharma Ag | Method and membrane for tissue regeneration |
| CN101884602A (en) * | 2010-03-03 | 2010-11-17 | 孙杰 | A kind of cosmetic formulations of skin-care, defect-repairing, anti-aging and winkle-removing |
| CN102178617A (en) * | 2011-05-09 | 2011-09-14 | 刘佩华 | Protein skin care products having function of skin repair and care |
Family Cites Families (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1319605C (en) * | 2005-12-07 | 2007-06-06 | 浙江大学 | Collagen-chitin and silicon rubber bilayer skin regeneration support and its preparation method |
-
2015
- 2015-06-24 CN CN201810074599.1A patent/CN108079372A/en not_active Withdrawn
- 2015-06-24 CN CN201510352562.7A patent/CN104984390B/en active Active
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1255843A (en) * | 1997-05-13 | 2000-06-07 | 韦尔豪泽公司 | Reticulated absorbent composite |
| CN101507834A (en) * | 2008-07-14 | 2009-08-19 | 昆明理工大学 | Degradable skin tissue engineering bracket material and preparation method thereof |
| WO2010082138A2 (en) * | 2009-01-16 | 2010-07-22 | Geistlich Pharma Ag | Method and membrane for tissue regeneration |
| CN101884602A (en) * | 2010-03-03 | 2010-11-17 | 孙杰 | A kind of cosmetic formulations of skin-care, defect-repairing, anti-aging and winkle-removing |
| CN102178617A (en) * | 2011-05-09 | 2011-09-14 | 刘佩华 | Protein skin care products having function of skin repair and care |
Non-Patent Citations (3)
| Title |
|---|
| 刘卉等: "《化妆品应用基础 人物形象设计系列》", 31 January 2011, 中国轻工业出版社 * |
| 杨金生等: "《中医刮痧师》", 30 November 2009, 中国中医药出版社 * |
| 王吾如等: "《病理科主治医生734问-现代主治医生提高丛书》", 31 December 2000, 中国协和医科大学出版社 * |
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| Publication number | Publication date |
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| CN104984390A (en) | 2015-10-21 |
| CN104984390B (en) | 2018-02-02 |
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