CN108077543A - A kind of preparation method of chewing gum for reducing blood pressure - Google Patents
A kind of preparation method of chewing gum for reducing blood pressure Download PDFInfo
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- CN108077543A CN108077543A CN201810092497.2A CN201810092497A CN108077543A CN 108077543 A CN108077543 A CN 108077543A CN 201810092497 A CN201810092497 A CN 201810092497A CN 108077543 A CN108077543 A CN 108077543A
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- blood pressure
- chewing gum
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Classifications
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23G—COCOA; COCOA PRODUCTS, e.g. CHOCOLATE; SUBSTITUTES FOR COCOA OR COCOA PRODUCTS; CONFECTIONERY; CHEWING GUM; ICE-CREAM; PREPARATION THEREOF
- A23G4/00—Chewing gum
- A23G4/06—Chewing gum characterised by the composition containing organic or inorganic compounds
- A23G4/14—Chewing gum characterised by the composition containing organic or inorganic compounds containing peptides or proteins
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23G—COCOA; COCOA PRODUCTS, e.g. CHOCOLATE; SUBSTITUTES FOR COCOA OR COCOA PRODUCTS; CONFECTIONERY; CHEWING GUM; ICE-CREAM; PREPARATION THEREOF
- A23G4/00—Chewing gum
- A23G4/06—Chewing gum characterised by the composition containing organic or inorganic compounds
- A23G4/068—Chewing gum characterised by the composition containing organic or inorganic compounds containing plants or parts thereof, e.g. fruits, seeds, extracts
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23G—COCOA; COCOA PRODUCTS, e.g. CHOCOLATE; SUBSTITUTES FOR COCOA OR COCOA PRODUCTS; CONFECTIONERY; CHEWING GUM; ICE-CREAM; PREPARATION THEREOF
- A23G4/00—Chewing gum
- A23G4/06—Chewing gum characterised by the composition containing organic or inorganic compounds
- A23G4/16—Chewing gum characterised by the composition containing organic or inorganic compounds containing dairy products
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P21/00—Preparation of peptides or proteins
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P21/00—Preparation of peptides or proteins
- C12P21/06—Preparation of peptides or proteins produced by the hydrolysis of a peptide bond, e.g. hydrolysate products
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P39/00—Processes involving microorganisms of different genera in the same process, simultaneously
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
Abstract
The present invention provides a kind of preparation method of chewing gum for reducing blood pressure, including sweetener, chewing gum base, fragrance, pigment and material for lowering blood pressure, it is to add material for lowering blood pressure under the premise of existing chewing gum ingredients, the material for lowering blood pressure is that the ace inhibitory peptide prepared in dairy produce is added to using shellfish meat and green tea powder compounding, improve the utilization rate and extraction efficiency of enzyme, make ace inhibitory peptide activity high, the present invention is to ferment by using bacillus subtilis and aspergillus niger to shellfish meat, aid in improving the activity and purity of ace inhibitory peptide using magnetic carbosphere, improve fermentation rate, ace inhibitory peptide is purified finally by magnetic silica adsorption column, obtain product.The ace inhibitory peptide activity of the present invention is high, and inhibiting rate is high, has good hypotensive activity, makes the chewing gum of preparation have good blood pressure reduction effect.
Description
Technical field
The invention belongs to food processing fields, and in particular to a kind of chewing gum and its preparation side with antihypertensive function
Method.
Background technology
Hypertension（hypertension）Refer to systemic arterial blood pressure（Systolic pressure and/or diastolic pressure）Increase to be main
Feature（The millimetres of mercury of systolic pressure >=140, the millimetres of mercury of diastolic pressure >=90）, can be with the function of the organs such as the heart, brain, kidney or device matter
Property damage clinical syndrome.At present, though clinically used blood-pressure drug has good antihypertensive effect, exist simultaneously
Toxic side effect and adverse reaction.
In terms of the pathogenesis of hypertension, inactive angiotensin I is in angiotensin converting enzyme
（Angiotensin I-converting enzyme, ACE) it is changed into the blood vessel with strong vasoconstrictor activity under catalytic action
Angiotensin Converting Enzyme II, bradykinin of degrading, makes parteriole vascular smooth muscle contraction, is raised so as to cause blood pressure.Oshima etc. from
Food-borne ace inhibitory peptide is extracted earliest in the bacterial collagenase digestive juice of gelatin, can effectively be inhibited ACE activity, be prevented blood
The generation of angiotensin II, so as to have the function that blood pressure lowering.Hereafter, scholars expand ace inhibitory peptide extensive research,
And it is found that ace inhibitory peptide in a variety of food proteins.Food-based ace inhibitory peptide antihypertensive effect is notable, safe,
It has no toxic side effect, and it is at low cost, it easily absorbs, more meets the demand of patient.
Due to the particularity of marine organism growth environment, searching hypotensive activity substance has become ocean from marine organisms
Drug, the important directions of oceanic functional food research.Clam is the common full of nutrition and relative low price in coastal area
Seashell products, at present to clam ace inhibitory peptide mostly using enzymatic isolation method extract, with Production by Microorganism Fermentation clam ACE inhibit
The report of peptide is less.
Milk nutritive value is high, and trophic component is in addition to lactose, dry matter, fat, protein, calcium, phosphorus and vitamin A etc.
Content is very high, close to human milk, prepared from breast ace inhibitory peptide, convenient sources safety, and ace inhibitory peptide can and its
He is absorbed by the body at nutritional ingredient jointly.
Chewing gum is the carbohydrate that a kind of many people are happy to chewing, but existing chewing gum is mostly examined with the single function that cleans the teeth
Consider the food of production.At present, also there are some new chewing gum formulations from increase chewing gum healthcare function purpose proposition, such as
Patent of invention CN93105577.6, Sarcandra glaber chewing gum and preparation method thereof, containing 0.1~20% Herba Pileae Scriptae extract, 10~
21% edible rubber, 50~64% sweeteners, 1~4% filler, 5~15% edible softening agents, 0.1~0.4% edible perfume
Essence can also add in 0.1~0.4% peppermint oil and 0.1~0.8% menthol.The production method of the chewing gum and common mouth
Fragrant sugar is essentially identical, and Herba Pileae Scriptae extract adds in simultaneously with essence.The chewing gum has antibacterial, anti-inflammatory, analgesic, the work of hemostasis
With, opposite, mouth, tooth, larynx have health care and therapeutic effect, also with antitumaous effect, and in good taste, free from extraneous odour, it is without side-effects.
Patent of invention CN201610528566.0, a kind of reducing blood pressure and blood fat health-care chewing gum and preparation method thereof, including
The chewing gum made of matrix, sugar and auxiliary material further includes the life of the tea polyphenols, 0.01-1% that account for chewing gum total weight 0.01-1%
Object active peptide, the calcium activated of 0.01-1%, the haw thorn extract of 0.01-1%, the cassia seed extract of 0.01-1%, 0.01-
1% Rhizoma Gastrodiae extract, the Chinese toon extract of 0.01-1%, the peanut leaf extract of 0.01-1%, the pueraria lobata of 0.01-1% carry
Take the crowndaisy chrysanthemum extract of object, 0.05-0.5%.This application introduced in modern food biotechnology tea polyphenols, Radix Notoginseng, calcium activated,
The functional components such as biologically active peptide, with reference to antihypertensive drugs such as hawthorn, Rhizoma Gastrodiae, cassia seeds, emphasis solves to utilize biotechnology hand
Section and nanometer technology ensure the bioactivity of additive or the lasting release of stability and fragrance, realize and are easily chewing
Make functional activity factor persistence simultaneously, generate steady, lasting antihypertensive effect, enhance the compliance of hyperpietic's medication,
Especially suitable for light, moderate essential hypertension prevention and the auxiliary treatment of severe hypertension disease.
At present, with the improvement of living standards, high in fat, the high protein of eating patterns cause more people in the middle age
The diseases such as hyperlipidemia and hypertension have just been suffered from, the normal life of this kind of crowd and spiritual mood has been seriously affected, and then has affected him
Work and cause, the remedy measures taken be the Related Drug class for often taking reducing blood lipid blood pressure lowering.First, not side of taking medicine
Just, second is that the carrying management of medicine is inconvenient, third, the sense of taste of medicine does not receive for majority, thus take medicine into passive behavior.Usually
It is difficult to often adhere to, some is caused only really to feel not quite the thing, has more prominent symptomatic reaction, just remembers medication.
Chewing gum has easy to carry, taste good easily the characteristics of taking, and chewing gum is prepared into antihypertensive function
Product rare report.
The content of the invention
For the deficiencies in the prior art, the present invention provides a kind of preparation side of the chewing gum with antihypertensive function
Method.
The present invention is achieved by the following technical solutions：
A kind of chewing gum for reducing blood pressure, including sweetener, chewing gum base, fragrance, pigment and material for lowering blood pressure, in existing chewing gum
Material for lowering blood pressure is added under the premise of dispensing and forms chewing gum for reducing blood pressure, according to ratio of weight and number, by material for lowering blood pressure 25 ~ 35
Part, 75 ~ 95 parts of chewing gum base is prepared, and specific method is as follows：
A. after fresh shellfish meat boiling is taken to shred, -20 DEG C of refrigerator freezings is placed in, are transferred to after 6h in vacuum freeze drier, freezing is dry
Dry 48h crushed 40 mesh sieves and obtain shellfish meat dry powder；
B. dry green tea is taken to wear into fine powder, the tannase that the mass fraction for then spraying tea quality 20 ~ 30% is 6 ~ 8% is molten
Liquid stands 40 ~ 60min of reaction, obtains green tea enzymolysis liquid after mixing；
C. shellfish meat dry powder is pressed into solid-liquid ratio 1:（3~4）It adds in distilled water to be homogenized, it is 6.3 ~ 7.0 to adjust pH, in parts by weight
Than, 20 ~ 40 parts of green tea enzymolysis liquids are added in, 60 ~ 80 parts of raw milks are uniformly mixed, and then add in the magnetic carbosphere of 40 ~ 60mg/ml,
After 20 ~ 30min of high pressure sterilization, culture medium is obtained；
The preparation method of the magnetism carbosphere is as follows：
（1）By useless bean dregs and potassium carbonate according to mass ratio 1:2 are dissolved in distilled water, impregnate 4h at room temperature, then dry to containing
Water is 2% ~ 5%, is moved into tube furnace, and under the protection of 400ml/min nitrogen, 600 ~ 650 are heated to the rate of 6 DEG C/min
DEG C, 75 ~ 100min is kept the temperature, sample is washed till neutrality after cooling, dry and grinds cross 50 ~ 100 mesh sieves, obtain activated carbon powder
End；
（2）By ratio of weight and the number of copies, 1 ~ 3 part of Fe is taken3O4Microballoon and 20 ~ 60 parts of active carbon powders, ultrasonic disperse is in 15 ~ 45 parts of distillations
In water, measure 0.5 ~ 1.5 part of concentrated ammonia liquor and add in above-mentioned system, 10min is stirred by ultrasonic；Above-mentioned mixed liquor is transferred to reaction kettle
In, in 180 C pyroreaction 4h, separated with magnet and collect the magnetic carbosphere of gained, washed successively with absolute ethyl alcohol and distillation
It washs several times, is dried under 60 C, obtain magnetic carbosphere；
D. in the composite bacteria liquid of inoculation of medium 8% ~ 10%, at 37 ~ 40 DEG C, shaking table culture 24 under the conditions of 180 ~ 200r/min ~
30h obtains zymotic fluid；
The composite bacteria liquid is bacillus 1389 and aspergillus niger AS3.350；
E. magnetic carbosphere is isolated under externally-applied magnetic field, then washs magnetic carbosphere nothing into cleaning solution repeatedly with distilled water
Protein substance collects cleaning solution；Zymotic fluid after cleaning solution and the magnetic carbosphere of separation is carried out being mixed to get mixed liquor, according to
Mixed liquor：Magnetic silica volume ratio=1:2nd, adsorbed under the conditions of flow velocity 1BV/h by magnetic silica adsorption column,
Gained eluent be concentrated into 0.6 ~ 0.8 times of original volume, material for lowering blood pressure is can obtain after pasteurization；
The magnetic silica absorption column preparation method is as follows：
（1）Take Fe3O4For microballoon ultrasonic disperse in toluene, 350rpm electric stirrings are respectively added slowly to volume of toluene 3% ~ 5%
Ethyl orthosilicate and triethylamine are stirred to react for 24 hours at room temperature, are separated with magnet and are collected gained magnetic silica microballoon, according to
It is secondary to use absolute ethyl alcohol and distillation water washing several times, it is dried under 60 C, obtains magnetic silica microballoon；
（2）By ratio of weight and the number of copies, weigh 20 ~ 40 parts of magnetic silica microballoon ultrasonic disperses in 400 ~ 600 parts of pH be 8.2
In Tris-HCl buffer solutions, then 350rpm electric stirrings are slowly added to 3- into system（Methacrylyl）Propyl trimethoxy
Base silane（MPS）And ethyl orthosilicate（TEOS）, react 16h at room temperature, separated with magnet and collect gained it is acryl-modified
Magnetic silica microballoon successively with absolute ethyl alcohol and distillation water washing several times, dries under 60 C, obtains amino modified magnetic
Property silicon dioxide microsphere；
（3）Above-mentioned amino modified magnetic silica microballoon is filled in adsorption column, loading is 80% ~ 90%, obtains dioxy
SiClx adsorption column；
F. by ratio of weight and the number of copies, 25 ~ 35 parts of material for lowering blood pressure is taken, 75 ~ 95 parts of chewing gum base is prepared into chewing gum for reducing blood pressure.
Preferably, the Fe3O4Method for preparing microsphere is as follows：
By ratio of weight and the number of copies, 2 ~ 4 parts of FeCl are weighed3·6H2O is dissolved in 60 ~ 80 parts of ethylene glycol, adds 6 ~ 8 parts of anhydrous sodium acetates,
2 ~ 4 parts of polyethylene glycol are added, reaction mixture is added in autoclave, 180 C high by ultrasonic 5min, magnetic agitation 15min
Temperature reaction 8h, magnetic source particle is separated with magnet（Fe3O4）Product, successively with absolute ethyl alcohol, water washing 3-5 times, 60 C drying obtains
To Fe3O4Microballoon.
During synthesizing magnetic microballoon, increase band active function groups silane coupling agent dosage, be prepared into magnetic microsphere pair
Protein adsorption quantity increases, and protein active conservation rate declines, in addition, active group silane coupling agent and ethyl orthosilicate amount ratio
Increase to 1 ~ 2:When 1, magnetic microsphere substantially rises protein adsorption quantity, and immobilization protein active conservation rate slightly decreases, former
Because be appropriate ankyrin not caused by very big steric hindrance.Therefore, synthesis modification magnetic microsphere is optimal silane coupled
Agent usage ratio is（1~2）:1.
The activated carbon prepared with bean dregs has the adsorption function of fine albumen, but does not adsorb peptide, can effectively adsorb compound bacteria
Ferment the enzyme generated, and bacillus and black song can be improved by improving enzyme activity and enzyme stability, the high microsteping of bean dregs activated carbon surface
Mould reproduction speed greatly improves fermentation efficiency, improves shellfish meat active peptide yield, is prepared using magnetic material and bean dregs activated carbon
Into magnetic carbosphere, beneficial to the separation of ace inhibitory peptide and foreign protein, improve the activity of peptide for inhibiting.
Bean dregs are carbonized, are prepared into the absorbent charcoal material of microcellular structure, the fishy smell in shellfish meat fermentation process can be adsorbed, are improved
The functionality of product.
Bean dregs belong to the by-product of bean product production process, easily go mouldy in storing process, corruption, especially in the summer
My god.Rotten bean dregs cannot act as animal feed, is often dropped or burns, so as to threaten environment structure.Fully profit of the invention
With bean dregs, raw material availability is improved.
The catechin contained in green tea can promote the metabolic capability of compound bacteria, so as to improve utilization of the compound bacteria to substrate
Rate, while catechin can promote to digest and assimilate, and human body is made to have better absorptivity to material for lowering blood pressure prepared by the present invention, is carried
The high value of the present invention.
Magnetic silica has the characteristics that stability is high, after amido modified, can improve its adsorption energy to albumen
Power, but repel each other to peptide molecule, so as to separate foreign protein and peptide for inhibiting, the foreign protein activity of magnetic silica absorption is high, absorption
Amount is high, makes the albumen after absorption（The enzyme that predominantly compound bacteria generates）It can be re-used for shellfish meat degradation or available for its other party
The production in face；The stability of silicon materials makes it that can repeatedly utilize, and the introducing of magnetic material is easily isolated, and reduces operation difficulty
And cost.
Preferably, step（3）Bacillus 1389 and aspergillus niger AS3.350 ratios are 2 in the composite bacteria liquid:1.
Preferably, in the preparation method of the magnetic carbosphere, Fe3O4Microballoon and active carbon powder mass ratio are 1:20, it steams
Distilled water and concentrated ammonia liquor volume ratio are 30:1, distilled water addition is 15ml/g active carbon powders.
Preferably, in the magnetic silica absorption column preparation method, Fe in system3O4Microspheres amount is 4mg/ml first
Benzene；Ethyl orthosilicate and triethylamine addition ratio are 1：1；
Preferably, in the magnetic silica absorption column preparation method, 3-（Methacrylyl）Propyl trimethoxy silicane
（MPS）And ethyl orthosilicate（TEOS）Amount ratio is（1~2）：1；Fe in system3O4Microballoon：3-（Methacrylyl）Propyl front three
Oxysilane（MPS）=1mg：2~4ml.
Preferably, the raw milk is one kind in fresh goat milk, milk.
Contain abundant nutriment in sheep, cow's milk liquid, the speed of growth of bacterium can not only be improved, while compound bacteria utilizes breast
The prolease activity higher that liquid fermentation generates promotes utilization of the bacterium to shellfish meat, makes the ace inhibitory peptide in the material for lowering blood pressure of preparation
Inhibiting rate higher；The distinctive fragrance of lotion can improve the fragrance of chewing gum, while lotion albumen can improve the mouthfeel of chewing gum, make
The chewing gum of preparation has preferably edible experience.
As a further improvement on the present invention, magnetic silica adsorption column carries out 2 ~ 3 production cycles of absorption in step E
Afterwards, using the distillation water washing adsorption column of 2BV/h, eluent is collected, powder is dried to, is then added in step B and shellfish meat
Dry powder blend prepares culture medium, and addition is the 65 ~ 80% of shellfish meat dry powder amount.
Beneficial effects of the present invention：
1st, material for lowering blood pressure ace inhibitory peptide is prepared with compound bacteria-fermented shellfish meat and lotion, by complex enzyme for hydrolyzing albumen, improved
The activity of ace inhibitory peptide reduces production difficulty, improves the degree of safety of product；The catechin contained in green tea can improve compound
Bacterium promotes absorption of the human body to peptide for inhibiting to the utilization rate of substrate, improves the nutritive value of chewing gum of the present invention；
2nd, the ace inhibitory peptide purity higher prepared with magnetic carbosphere combination compound bacteria-fermented, improves fermentation efficiency, is more easy to point
From foreign protein and peptide for inhibiting；Nutriment in lotion improve compound bacteria efficiency and bacterium to the degree of degradation of shellfish meat albumen, contracting
Short fermentation period；
3rd, isolating and purifying for ace inhibitory peptide is carried out with magnetic silica adsorption column, reduces operation difficulty, separated protein active
Height, predominantly enzyme caused by compound bacteria, can be used for Xun Huan degradation shellfish meat and lotion prepares ace inhibitory peptide or can be used for
Other productions, improve raw material availability.
Specific embodiment
With reference to embodiment, the invention will be further described.
Embodiment 1
A. after fresh shellfish meat boiling is taken to shred, -20 DEG C of refrigerator freezings is placed in, are transferred to after 6h in vacuum freeze drier, freezing is dry
Dry 48h crushed 40 mesh sieves and obtain shellfish meat dry powder；
B. dry green tea is taken to wear into fine powder, the mass fraction for then spraying tea quality 20% is 6% tannin enzyme solutions, is mixed
Reaction 40min is stood after conjunction, obtains green tea enzymolysis liquid；
C. shellfish meat dry powder is pressed into solid-liquid ratio 1:3 addition distilled water are homogenized, and it is 6.3 to adjust pH, by ratio of weight and the number of copies, add in 20
Part green tea enzymolysis liquid, 60 parts of raw milks are uniformly mixed, then the magnetic carbosphere of addition 40mg/ml, after high pressure sterilization 20min, obtain
To culture medium；
The preparation method of the magnetism carbosphere is as follows：
（1）By useless bean dregs and potassium carbonate according to mass ratio 1:2 are dissolved in distilled water, impregnate 4h at room temperature, then dry to containing
Water is 2%, is moved into tube furnace, under the protection of 400ml/min nitrogen, is heated to 600 DEG C with the rate of 6 DEG C/min, heat preservation
75min, neutrality is washed till after cooling by sample, and dry and grinds cross 50 mesh sieves, obtain activated carbon powder；
（2）By ratio of weight and the number of copies, 1 part of Fe is taken3O4Microballoon and 20 parts of active carbon powders, ultrasonic disperse is in 15 parts of distilled water, amount
0.5 part of concentrated ammonia liquor is taken to add in above-mentioned system, 10min is stirred by ultrasonic；Above-mentioned mixed liquor is transferred in reaction kettle, in 180 C
Pyroreaction 4h is separated with magnet and is collected the magnetic carbosphere of gained, successively with absolute ethyl alcohol and distillation water washing several times, in
It is dried under 60 C, obtains magnetic carbosphere；
D. in the composite bacteria liquid of inoculation of medium 8%, at 37 DEG C, shaking table culture for 24 hours, obtains zymotic fluid under the conditions of 180r/min；
The composite bacteria liquid is bacillus 1389 and aspergillus niger AS3.350；
E. magnetic carbosphere is isolated under externally-applied magnetic field, then washs magnetic carbosphere nothing into cleaning solution repeatedly with distilled water
Protein substance collects cleaning solution；Zymotic fluid after cleaning solution and the magnetic carbosphere of separation is carried out being mixed to get mixed liquor, according to
Mixed liquor：Magnetic silica volume ratio=1:2nd, adsorbed under the conditions of flow velocity 1BV/h by magnetic silica adsorption column,
Gained eluent be concentrated into 0.6 times of original volume, material for lowering blood pressure is can obtain after pasteurization；
The magnetic silica absorption column preparation method is as follows：
（1）Take Fe3O4For microballoon ultrasonic disperse in toluene, 350rpm electric stirrings are being respectively added slowly to volume of toluene 3% just
Silester and triethylamine are stirred to react for 24 hours at room temperature, are separated with magnet and are collected gained magnetic silica microballoon, successively
With absolute ethyl alcohol and distillation water washing several times, dried under 60 C, obtain magnetic silica microballoon；
Fe in system3O4Microspheres amount is 4mg/ml toluene；Ethyl orthosilicate and triethylamine addition ratio are 1：1；
（2）By ratio of weight and the number of copies, 20 parts of magnetic silica microballoon ultrasonic disperses are weighed in the Tris-HCl that 400 parts of pH are 8.2
In buffer solution, then 350rpm electric stirrings are slowly added to 3- into system（Methacrylyl）Propyl trimethoxy silicane
（MPS）And ethyl orthosilicate（TEOS）, 16h is reacted at room temperature, is separated with magnet and is collected gained acryl-modified magnetic two
Silicon oxide microsphere successively with absolute ethyl alcohol and distillation water washing several times, dries under 60 C, obtains amino modified magnetic dioxy
SiClx microballoon；
The 3-（Methacrylyl）Propyl trimethoxy silicane（MPS）And ethyl orthosilicate（TEOS）Amount ratio is 1：1；System
Middle Fe3O4Microballoon：3-（Methacrylyl）Propyl trimethoxy silicane（MPS）=1mg：2ml；
（3）Above-mentioned amino modified magnetic silica microballoon is filled in adsorption column, loading 90% obtains silica
Adsorption column；
F. by ratio of weight and the number of copies, 25 parts of material for lowering blood pressure is taken, 75 parts of chewing gum base is prepared into chewing gum for reducing blood pressure.
The Fe3O4Method for preparing microsphere is as follows：
By ratio of weight and the number of copies, 2 parts of FeCl are weighed3•6H2O is dissolved in 60 parts of ethylene glycol, is added 6 parts of anhydrous sodium acetates, is added 2
Reaction mixture is added in autoclave by part polyethylene glycol, ultrasonic 5min, magnetic agitation 15min, 180 C pyroreaction 8h,
Magnetic source particle is separated with magnet（Fe3O4）Product, successively with absolute ethyl alcohol, water washing 3 times, 60 C drying obtains Fe3O4Microballoon.
After magnetic silica adsorption column carries out 3 production cycles of absorption in step E, the distillation water washing of 2BV/h is utilized
Adsorption column collects eluent, is dried to powder, is then added in step B and prepares culture medium, addition with shellfish meat dry powder blend
For the 65% of shellfish meat dry powder amount.
Embodiment 2
A. after fresh shellfish meat boiling is taken to shred, -20 DEG C of refrigerator freezings is placed in, are transferred to after 6h in vacuum freeze drier, freezing is dry
Dry 48h crushed 40 mesh sieves and obtain shellfish meat dry powder；
B. dry green tea is taken to wear into fine powder, the mass fraction for then spraying tea quality 30% is 8% tannin enzyme solutions, is mixed
Reaction 60min is stood after conjunction, obtains green tea enzymolysis liquid；
C. shellfish meat dry powder is pressed into solid-liquid ratio 1:4 addition distilled water are homogenized, and it is 7.0 to adjust pH, by ratio of weight and the number of copies, add in 40
Part green tea enzymolysis liquid, 80 parts of raw milks are uniformly mixed, then the magnetic carbosphere of addition 60mg/ml, after high pressure sterilization 30min,
Obtain culture medium；
The preparation method of the magnetism carbosphere is as follows：
（1）By useless bean dregs and potassium carbonate according to mass ratio 1:2 are dissolved in distilled water, impregnate 4h at room temperature, then dry to containing
Water is 5%, is moved into tube furnace, under the protection of 400ml/min nitrogen, is heated to 650 DEG C with the rate of 6 DEG C/min, heat preservation
100min, neutrality is washed till after cooling by sample, and dry and grinds cross 100 mesh sieves, obtain active carbon powder；
（2）By ratio of weight and the number of copies, 3 parts of Fe are taken3O4Microballoon and 60 parts of active carbon powders, ultrasonic disperse is in 45 parts of distilled water, amount
1.5 parts of concentrated ammonia liquors is taken to add in above-mentioned system, 10min is stirred by ultrasonic；Above-mentioned mixed liquor is transferred in reaction kettle, in 180 C
Pyroreaction 4h is separated with magnet and is collected the magnetic carbosphere of gained, successively with absolute ethyl alcohol and distillation water washing several times, in
It is dried under 60 C, obtains magnetic carbosphere；
D. in the composite bacteria liquid of inoculation of medium 8% ~ 10%, at 37 ~ 40 DEG C, shaking table culture 30h under the conditions of 200r/min is obtained
Zymotic fluid；
The composite bacteria liquid is bacillus 1389 and aspergillus niger AS3.350；
E. magnetic carbosphere is isolated under externally-applied magnetic field, then washs magnetic carbosphere nothing into cleaning solution repeatedly with distilled water
Protein substance collects cleaning solution；Zymotic fluid after cleaning solution and the magnetic carbosphere of separation is carried out being mixed to get mixed liquor, according to
Mixed liquor：Magnetic silica volume ratio=1:2nd, adsorbed under the conditions of flow velocity 1BV/h by magnetic silica adsorption column,
Gained eluent be concentrated into 0.8 times of original volume, material for lowering blood pressure is can obtain after pasteurization；
The magnetic silica absorption column preparation method is as follows：
（1）Take Fe3O4For microballoon ultrasonic disperse in toluene, 350rpm electric stirrings are being respectively added slowly to volume of toluene 5% just
Silester and triethylamine are stirred to react for 24 hours at room temperature, are separated with magnet and are collected gained magnetic silica microballoon, successively
With absolute ethyl alcohol and distillation water washing several times, dried under 60 C, obtain magnetic silica microballoon；
Fe in system3O4Microspheres amount is 4mg/ml toluene；Ethyl orthosilicate and triethylamine addition ratio are 1：1；
（2）By ratio of weight and the number of copies, 40 parts of magnetic silica microballoon ultrasonic disperses are weighed in the Tris-HCl that 600 parts of pH are 8.2
In buffer solution, then 350rpm electric stirrings are slowly added to 3- into system（Methacrylyl）Propyl trimethoxy silicane
（MPS）And ethyl orthosilicate（TEOS）, 16h is reacted at room temperature, is separated with magnet and is collected gained acryl-modified magnetic two
Silicon oxide microsphere successively with absolute ethyl alcohol and distillation water washing several times, dries under 60 C, obtains amino modified magnetic dioxy
SiClx microballoon；
The 3-（Methacrylyl）Propyl trimethoxy silicane（MPS）And ethyl orthosilicate（TEOS）Amount ratio is 2：1；System
Middle Fe3O4Microballoon：3-（Methacrylyl）Propyl trimethoxy silicane（MPS）=1mg：4ml；
（3）Above-mentioned amino modified magnetic silica microballoon is filled in adsorption column, loading 80% obtains silica
Adsorption column；
F. by ratio of weight and the number of copies, 35 parts of material for lowering blood pressure is taken, 95 parts of chewing gum base is prepared into chewing gum for reducing blood pressure.
The Fe3O4Method for preparing microsphere is as follows：
By ratio of weight and the number of copies, 4 parts of FeCl3 6H2O are weighed to be dissolved in 80 parts of ethylene glycol, 8 parts of anhydrous sodium acetates is added, adds 4
Reaction mixture is added in autoclave by part polyethylene glycol, ultrasonic 5min, magnetic agitation 15min, 180 C pyroreaction 8h,
Magnetic source particle is separated with magnet（Fe3O4）Product, successively with absolute ethyl alcohol, water washing 5 times, 60 C drying obtains Fe3O4Microballoon.
After magnetic silica adsorption column carries out 2 production cycles of absorption in step E, the distillation water washing of 2BV/h is utilized
Adsorption column collects eluent, is dried to powder, is then added in step B and prepares culture medium, addition with shellfish meat dry powder blend
For the 80% of shellfish meat dry powder amount；
Embodiment 3
A. after fresh shellfish meat boiling is taken to shred, -20 DEG C of refrigerator freezings is placed in, are transferred to after 6h in vacuum freeze drier, freezing is dry
Dry 48h crushed 40 mesh sieves and obtain shellfish meat dry powder；
B. dry green tea is taken to wear into fine powder, the mass fraction for then spraying tea quality 25% is 7% tannin enzyme solutions, is mixed
Reaction 50min is stood after conjunction, obtains green tea enzymolysis liquid；
C. shellfish meat dry powder is pressed into solid-liquid ratio 1:4 addition distilled water are homogenized, and it is 6.5 to adjust pH, by quality parts ratio, add in 25
Part green tea enzymolysis liquid, 75 parts of raw milks are uniformly mixed, then the magnetic carbosphere of addition 55mg/ml, after high pressure sterilization 30min, obtain
To culture medium；
The preparation method of the magnetism carbosphere is as follows：
（1）By useless bean dregs and potassium carbonate according to mass ratio 1:2 are dissolved in distilled water, impregnate 4h at room temperature, then dry to containing
Water is 4%, is moved into tube furnace, under the protection of 400ml/min nitrogen, is heated to 600 DEG C with the rate of 6 DEG C/min, heat preservation
80min, neutrality is washed till after cooling by sample, and dry and grinds cross 80 mesh sieves, obtain activated carbon powder；
（2）By ratio of weight and the number of copies, 2 parts of Fe are taken3O4Microballoon and 40 parts of active carbon powders, ultrasonic disperse is in 30 parts of distilled water, amount
1 part of concentrated ammonia liquor is taken to add in above-mentioned system, 10min is stirred by ultrasonic；Above-mentioned mixed liquor is transferred in reaction kettle, in 180 C high
Temperature reaction 4h is separated with magnet and is collected the magnetic carbosphere of gained, successively with absolute ethyl alcohol and distillation water washing several times, in 60
It is dried under C, obtains magnetic carbosphere；
D. in the composite bacteria liquid of inoculation of medium 9%, at 38 DEG C, shaking table culture 28h under the conditions of 200r/min obtains zymotic fluid；
The composite bacteria liquid is bacillus 1389 and aspergillus niger AS3.350；
E. magnetic carbosphere is isolated under externally-applied magnetic field, then washs magnetic carbosphere repeatedly with distilled water into cleaning solution
Without protein substance, cleaning solution is collected；Zymotic fluid after cleaning solution and the magnetic carbosphere of separation is carried out being mixed to get mixed liquor, is pressed
According to mixed liquor：Magnetic silica volume ratio=1:2nd, inhaled under the conditions of flow velocity 1BV/h by magnetic silica adsorption column
It is attached, gained eluent be concentrated into 0.7 times of original volume, material for lowering blood pressure is can obtain after pasteurization；
The magnetic silica absorption column preparation method is as follows：
（1）Take Fe3O4For microballoon ultrasonic disperse in toluene, 350rpm electric stirrings are being respectively added slowly to volume of toluene 4% just
Silester and triethylamine are stirred to react for 24 hours at room temperature, are separated with magnet and are collected gained magnetic silica microballoon, successively
With absolute ethyl alcohol and distillation water washing several times, dried under 60 C, obtain magnetic silica microballoon；
Fe in system3O4Microspheres amount is 4mg/ml toluene；Ethyl orthosilicate and triethylamine addition ratio are 1：1；
（2）By ratio of weight and the number of copies, 30 parts of magnetic silica microballoon ultrasonic disperses are weighed in the Tris-HCl that 500 parts of pH are 8.2
In buffer solution, then 350rpm electric stirrings are slowly added to 3- into system（Methacrylyl）Propyl trimethoxy silicane
（MPS）And ethyl orthosilicate（TEOS）, 16h is reacted at room temperature, is separated with magnet and is collected gained acryl-modified magnetic two
Silicon oxide microsphere successively with absolute ethyl alcohol and distillation water washing several times, dries under 60 C, obtains amino modified magnetic dioxy
SiClx microballoon；The 3-（Methacrylyl）Propyl trimethoxy silicane（MPS）And ethyl orthosilicate（TEOS）Amount ratio is 2：
1；Fe in system3O4Microballoon：3-（Methacrylyl）Propyl trimethoxy silicane（MPS）=1mg：3ml；
（3）Above-mentioned amino modified magnetic silica microballoon is filled in adsorption column, loading 85% obtains silica
Adsorption column；
F. by ratio of weight and the number of copies, 30 parts of material for lowering blood pressure is taken, 90 parts of chewing gum base is prepared into chewing gum for reducing blood pressure.
The Fe3O4Method for preparing microsphere is as follows：
By ratio of weight and the number of copies, 3 parts of FeCl3 6H2O are weighed to be dissolved in 70 parts of ethylene glycol, 7 parts of anhydrous sodium acetates is added, adds 3
Reaction mixture is added in autoclave by part polyethylene glycol, ultrasonic 5min, magnetic agitation 15min, 180 C pyroreaction 8h,
Magnetic source particle is separated with magnet（Fe3O4）Product, successively with absolute ethyl alcohol, water washing 4 times, 60 C drying obtains Fe3O4Microballoon.
After magnetic silica adsorption column carries out 3 production cycles of absorption in step E, the distillation water washing of 2BV/h is utilized
Adsorption column collects eluent, is dried to powder, is then added in step B and prepares culture medium, addition with shellfish meat dry powder blend
For the 70% of shellfish meat dry powder amount；
Each content of material of chewing gum such as table 1 prepared by embodiment 1 ~ 3.
1 embodiment of table, 1 ~ 3 each content of material of chewing gum
Material for lowering blood pressure effect such as table 2 prepared by embodiment 1 ~ 3.
2 embodiment of table, 1 ~ 3 material for lowering blood pressure effect
As shown in Table 2, there is chewing gum prepared by the present invention that there is very high hypotensive activity, in material for lowering blood pressure preparation process
In, enzyme activity conservation rate after magnetic silica adsorption column separation is high, available for circulating fermentation 2 ~ 3 times or more, has very
Good economic benefit.
Above example is only exemplary embodiment of the present invention, is not used in the limitation present invention, protection scope of the present invention
It is defined by the claims.Those skilled in the art can make the present invention various repair in the essence and protection domain of the present invention
Change or equivalent substitution, this modification or equivalent substitution also should be regarded as being within the scope of the present invention.
Claims (8)
1. a kind of chewing gum for reducing blood pressure, including sweetener, chewing gum base, fragrance, pigment and material for lowering blood pressure, feature exists
In, added under the premise of existing chewing gum ingredients material for lowering blood pressure form chewing gum for reducing blood pressure, according to ratio of weight and number, by dropping
25 ~ 35 parts of blood pressure substance, 75 ~ 95 parts of chewing gum base are prepared, and specific method is as follows：
A. after fresh shellfish meat boiling is taken to shred, -20 DEG C of refrigerator freezings is placed in, are transferred to after 6h in vacuum freeze drier, freezing is dry
Dry 48h crushed 40 mesh sieves and obtain shellfish meat dry powder；
B. dry green tea is taken to wear into fine powder, the tannase that the mass fraction for then spraying tea quality 20 ~ 30% is 6 ~ 8% is molten
Liquid stands 40 ~ 60min of reaction, obtains green tea enzymolysis liquid after mixing；
C. shellfish meat dry powder is pressed into solid-liquid ratio 1:（3~4）It adds in distilled water to be homogenized, it is 6.3 ~ 7.0 to adjust pH, in parts by weight
Than, 20 ~ 40 parts of green tea enzymolysis liquids are added in, 60 ~ 80 parts of raw milks are uniformly mixed, and then add in the magnetic carbosphere of 40 ~ 60mg/ml,
After 20 ~ 30min of high pressure sterilization, culture medium is obtained；
The preparation method of the magnetism carbosphere is as follows：
（1）By useless bean dregs and potassium carbonate according to mass ratio 1:2 are dissolved in distilled water, impregnate 4h at room temperature, then dry to containing
Water is 2% ~ 5%, is moved into tube furnace, and under the protection of 400ml/min nitrogen, 600 ~ 650 are heated to the rate of 6 DEG C/min
DEG C, 75 ~ 100min is kept the temperature, sample is washed till neutrality after cooling, dry and grinds cross 50 ~ 100 mesh sieves, obtain activated carbon powder
End；
（2）By ratio of weight and the number of copies, 1 ~ 3 part of Fe is taken3O4Microballoon and 20 ~ 60 parts of active carbon powders, ultrasonic disperse is in 15 ~ 45 parts of distillations
In water, measure 0.5 ~ 1.5 part of concentrated ammonia liquor and add in above-mentioned system, 10min is stirred by ultrasonic；Above-mentioned mixed liquor is transferred to reaction kettle
In, in 180 C pyroreaction 4h, separated with magnet and collect the magnetic carbosphere of gained, washed successively with absolute ethyl alcohol and distillation
It washs several times, is dried under 60 C, obtain magnetic carbosphere；
D. in the composite bacteria liquid of inoculation of medium 8% ~ 10%, at 37 ~ 40 DEG C, shaking table culture 24 under the conditions of 180 ~ 200r/min ~
30h obtains zymotic fluid；
The composite bacteria liquid is bacillus 1389 and aspergillus niger AS3.350；
E. magnetic carbosphere is isolated under externally-applied magnetic field, then washs magnetic carbosphere nothing into cleaning solution repeatedly with distilled water
Protein substance collects cleaning solution；Zymotic fluid after cleaning solution and the magnetic carbosphere of separation is carried out being mixed to get mixed liquor, according to
Mixed liquor：Magnetic silica volume ratio=1:2nd, adsorbed under the conditions of flow velocity 1BV/h by magnetic silica adsorption column,
Gained eluent be concentrated into 0.6 ~ 0.8 times of original volume, material for lowering blood pressure is can obtain after pasteurization；
The magnetic silica absorption column preparation method is as follows：
（1）Take Fe3O4For microballoon ultrasonic disperse in toluene, 350rpm electric stirrings are respectively added slowly to volume of toluene 3% ~ 5%
Ethyl orthosilicate and triethylamine are stirred to react for 24 hours at room temperature, are separated with magnet and are collected gained magnetic silica microballoon, according to
It is secondary to use absolute ethyl alcohol and distillation water washing several times, it is dried under 60 C, obtains magnetic silica microballoon；
（2）By ratio of weight and the number of copies, weigh 20 ~ 40 parts of magnetic silica microballoon ultrasonic disperses in 400 ~ 600 parts of pH be 8.2
In Tris-HCl buffer solutions, then 350rpm electric stirrings are slowly added to 3- into system（Methacrylyl）Propyl trimethoxy
Base silane（MPS）And ethyl orthosilicate（TEOS）, react 16h at room temperature, separated with magnet and collect gained it is acryl-modified
Magnetic silica microballoon successively with absolute ethyl alcohol and distillation water washing several times, dries under 60 C, obtains amino modified magnetic
Property silicon dioxide microsphere；
（3）Above-mentioned amino modified magnetic silica microballoon is filled in adsorption column, loading is 80% ~ 90%, obtains dioxy
SiClx adsorption column；
F. by ratio of weight and the number of copies, 25 ~ 35 parts of material for lowering blood pressure is taken, 75 ~ 95 parts of chewing gum base is prepared into chewing gum for reducing blood pressure.
A kind of 2. preparation method of chewing gum for reducing blood pressure according to claim 1, which is characterized in that the Fe3O4Microballoon system
Preparation Method is as follows：
By ratio of weight and the number of copies, 2 ~ 4 parts of FeCl are weighed3·6H2O is dissolved in 60 ~ 80 parts of ethylene glycol, adds 6 ~ 8 parts of anhydrous sodium acetates,
2 ~ 4 parts of polyethylene glycol are added, reaction mixture is added in autoclave, 180 C high by ultrasonic 5min, magnetic agitation 15min
Temperature reaction 8h, magnetic source particle is separated with magnet（Fe3O4）Product, successively with absolute ethyl alcohol, water washing 3 ~ 5 times, 60 C drying obtains
To Fe3O4Microballoon.
A kind of 3. preparation method of chewing gum for reducing blood pressure according to claim 1, which is characterized in that step（3）It is described multiple
It is 2 to close bacillus 1389 and aspergillus niger AS3.350 ratios in bacterium solution:1.
A kind of 4. preparation method of chewing gum for reducing blood pressure according to claim 1, which is characterized in that the magnetism carbosphere
Preparation method in, Fe3O4Microballoon and active carbon powder mass ratio are 1:20, distilled water and concentrated ammonia liquor volume ratio are 30:1, distillation
Water addition is 15ml/g active carbon powders.
A kind of 5. preparation method of chewing gum for reducing blood pressure according to claim 1, which is characterized in that the magnetism titanium dioxide
In silicon absorption column preparation method, Fe in system3O4Microspheres amount is 4mg/ml toluene；Ethyl orthosilicate and triethylamine addition ratio
For 1：1.
A kind of 6. preparation method of chewing gum for reducing blood pressure according to claim 1, which is characterized in that the magnetism titanium dioxide
In silicon absorption column preparation method, 3-（Methacrylyl）Propyl trimethoxy silicane（MPS）And ethyl orthosilicate（TEOS）Dosage
Than for（1~2）：1；Fe in system3O4Microballoon：3-（Methacrylyl）Propyl trimethoxy silicane（MPS）=1mg：2~4ml.
7. the preparation method of a kind of chewing gum for reducing blood pressure according to claim 1, which is characterized in that the raw milk is new
One kind in fresh goat milk, milk.
A kind of 8. preparation method of chewing gum for reducing blood pressure according to claim 1, which is characterized in that magnetism two in step E
After silica adsorption column carries out 2 ~ 3 production cycles of absorption, using the distillation water washing adsorption column of 2BV/h, eluent is collected, is done
It is dry to be then added in step B and prepare culture medium with shellfish meat dry powder blend into powder, addition for shellfish meat dry powder amount 65 ~
80%。
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| CN111557369A (en) * | 2020-05-29 | 2020-08-21 | 中南林业科技大学 | Chewing gum capable of quickly inhibiting fusobacterium nucleatum and preparation method thereof |
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Application publication date: 20180529 |