CN108064646A - A kind of method that basic element of cell division inspires bletilla striata bulb reproduction - Google Patents
A kind of method that basic element of cell division inspires bletilla striata bulb reproduction Download PDFInfo
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- CN108064646A CN108064646A CN201711279408.7A CN201711279408A CN108064646A CN 108064646 A CN108064646 A CN 108064646A CN 201711279408 A CN201711279408 A CN 201711279408A CN 108064646 A CN108064646 A CN 108064646A
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- bulb
- bletilla striata
- reproduction
- inspires
- cell division
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01C—PLANTING; SOWING; FERTILISING
- A01C1/00—Apparatus, or methods of use thereof, for testing or treating seed, roots, or the like, prior to sowing or planting
- A01C1/08—Immunising seed
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01C—PLANTING; SOWING; FERTILISING
- A01C1/00—Apparatus, or methods of use thereof, for testing or treating seed, roots, or the like, prior to sowing or planting
- A01C1/02—Germinating apparatus; Determining germination capacity of seeds or the like
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- Life Sciences & Earth Sciences (AREA)
- Soil Sciences (AREA)
- Environmental Sciences (AREA)
- Health & Medical Sciences (AREA)
- Physiology (AREA)
- Pretreatment Of Seeds And Plants (AREA)
Abstract
The invention belongs to Chinese medicine sapling multiplication technical fields, disclose a kind of method that basic element of cell division inspires bletilla striata bulb reproduction, and the method that the basic element of cell division inspires bletilla striata bulb reproduction includes：The pretreatment of bletilla striata bulb, using effect hormone carry out seed soaking to bletilla striata bulb, accelerate taking root, sprouting for bletilla striata bulb；Seedbed management is carried out, promotes the growth course of bletilla striata bulb.Present invention uses 6 benayl aminopurines to carry out chemicals treatment, accelerates the vitro growth rates of bletilla striata bud, can the growth time of conventional bletilla striata bulb reproduction be done sth. in advance 10 20 days；Simultaneously because the present invention is that the bulb of normal bulb reproduction is divided into two, therefore the bulb quantity of bletilla striata bulb reproduction can be reduced half or so.
Description
Technical field
It is numerous that bletilla striata bulb is inspired the invention belongs to Chinese medicine sapling multiplication technical field more particularly to a kind of basic element of cell division
The method grown.
Background technology
Although the bletilla striata can generate substantial amounts of seed (each fruit pod 10~300,000), bletilla striata seeds do not have endosperm,
It cannot normally be sprouted under natural conditions.Piecemeal stem breeding is substantially carried out in actual production.The bletilla striata is dug out at the beginning of the 9--11 months,
Stem tuber of medium size, of more buds, disease-free is selected, 1--2 bud of every piece of band is planted after being stained with plant ash.20---25 lis of drains spacing of trench digging
Rice, deep 5--6 centimetres, one, stem tuber is put for 10---12 centimetres by spacing in the rows, and bud is upward, fills out, and is compacted, and grass is covered in watering, often keeps
Humidity, the 3--4 months emerge.100 kilograms of seedling of mu.In its natural state the bletilla striata mainly by the formula of being divided into two division propagation, from
A small amount of satisfactory high-quality bletilla striata seedling starts nature and expands numerous and cultivate, and formation scale takes a long time, generally will be in 5--
The time of 6 months could complete.
It is still the stripping and slicing mode of extensive style for piecemeal stem breeding technology, randomness is larger, operation is extensive, causes seedling
Quality is irregular to differ, disease rate is high, planting percent is low, growth cycle is long, production cost is high, it is difficult to meet the needs in production.Profit
Seedling breeding level can be effectively improved with plant hormone technology, the achievement in research of this respect is considerably less at present, and achievement
Using essentially blank spot.
The basic element of cell division (cytokinin, CTK) is separated from corn or other plant or artificial synthesized plant hormone.
It is generally generated in plant root, is a kind of substance for promoting cytokinesis, promotes the differentiation and growth of Various Tissues.Cell division
There are two types of the most apparent physiological actions of element：When promote cell division and regulate and control its differentiation.In tissue cultures, the basic element of cell division
Plant organ differentiation in scale effect with auxin, when usual ratio is high, is conducive to the differentiation of bud；When ratio is low, be conducive to
The differentiation of root.；Second is that delaying the degradation of protein and chlorophyll, delay senility；Primarily now in the tissue cultures of plant
The production of tissue-cultured seedling is carried out, the flourish for being directly used in plant bulb is also fewer, effect is also than less obvious.It is main to use
6-benzyl aminopurine.
Since the equipment of production tissue-cultured seedling needs, technology requirement are all higher, vast farmer is made to be difficult to promote, make
With, thus this technology mainly solve bletilla striata seedling quality it is irregular differ, disease rate is high, planting percent is low, growth cycle is long, is produced into
This high production problem enables bletilla striata bulb reproduction technique to be applied well in production.
In conclusion problem existing in the prior art is：
In existing bletilla striata bulb reproduction technique, when the method taken in production is with bletilla striata bulb propagation, bletilla striata bulb propagation
Shi Shengchang is slow, generally requires time of more than half a year, while emergence rate is relatively low or even between 30--60%, it is difficult to meet
The needs of large area production.
Since bletilla striata growth is slow, development time is long, standardized planting technology is poor in growth course, for the physiology of the bletilla striata
Biochemical Research is seldom, therefore more lacks to carry out the content of physiological Study and progress productive experiment in terms of hormone.
The content of the invention
In view of the problems of the existing technology, the present invention provides the sides that a kind of basic element of cell division inspires bletilla striata bulb reproduction
Method.According to current state of development, the present invention, by the stimulation and processing of associated hormone, on the one hand induces in terms of bletilla striata physiology
The growth of bud is sprouted and accelerated to the bulb of the bletilla striata, on the other hand by functions of hormones, accelerates the speed of growth of the bletilla striata, carries
The emergence rate of high bletilla striata seedling.
The present invention solves the problems, such as that the bulb emergence rate of the bletilla striata is low, the consumption of bulb quantity is big simultaneously, can make bletilla seed
Ball reproduction technique becomes a kind of conventional seedbed system method in production, can effectively reduce production cost while also allow for big
It is applied in area production.The present invention also solves the problems, such as that the bulb speed of growth is low in bletilla striata bulb reproduction technique simultaneously,
Improve production efficiency.
The present invention is achieved in that a kind of method that basic element of cell division inspires bletilla striata bulb reproduction, the cell division
The method that element inspires bletilla striata bulb reproduction includes：
The pretreatment of bletilla striata bulb, using effect hormone carry out seed soaking to bletilla striata bulb, accelerate the life of bletilla striata bulb
Root, sprouting；
Seedbed management is carried out, promotes the growth course of bletilla striata bulb.
Further, the basic element of cell division inspires the method for bletilla striata bulb reproduction and specifically includes：
Growth selection is full, healthy bletilla striata bulb without disease pest harm, from bud head rip cutting, bud is made to divide multiple faces equally；
It will cut and be put into 500-800 times of carbendazim solution with bud bulb and carry out disinfection processing, dry spare；
The bulb dried be put into 20PPM methyl α-naphthyl acetate+10PPM6- benayl aminopurines mixed liquor carry out impregnate 20 minutes-
25 minutes；It dries；
It is cultivated the good bulb of chemicals treatment is dried into the seedbed disinfected；Planting seeding row spacing is：4 6~8 lis of cm x
Meter, the matrix of 0.5 centimetre~1 centimetre of covering, sprinkles profoundly water on bulb；
On the seedbed transplanted in bulb, water management is carried out；
Be spaced 48 it is small when~72 it is small when, toward seedbed land measure equal to fifteen mu in most parts of the Northeast face on spray a 10PPM6- benayl aminopurine liquid, until bud is opened
Until beginning to be unearthed.
Pay attention to：1st, it is described to disinfect the time as -10 minutes 5 minutes.
2nd, the cultivation matrix in the seedbed is made of by quality thin 5 parts of laterite, 2 parts of thin peat and husky 1 part respectively.
3rd, water management is：It is not dry do not pour, one irrigates；It is controlled in the entire growth period temperature of nursery 20 DEG C~25
℃。
Another object of the present invention is to provide a kind of basic element of cell division to inspire bletilla striata bulb propagating system.
Advantages of the present invention and good effect are：
1st, the present invention obtains 2 parts or more parts of bulb reproduction materials by dividing the terminal bud of the bletilla striata equally, can save half
Above bulb reproduction material；The probability of bulb introduced disease is reduced after disinfecting, makes bulb incidence 10%
Below.
2nd, the present invention handles the bletilla striata by methyl α-naphthyl acetate, can promote bulb take root it is rapid, grow, each bulb is taken root
Number can reach 1--3 roots, and the length of root is at 1.5 centimetres or more.Present invention uses 6-benzyl aminopurines to carry out chemicals treatment,
The bud and bulb vitro growth rates for making the bletilla striata are accelerated, and can the growth time of conventional bletilla striata bulb reproduction be done sth. in advance 10--20
My god.
3rd, the present invention is compared with conventional bulb reproduction technology, bulb cost savings more than half；Bletilla striata seedling goes out simultaneously
The seedling time can do sth. in advance 10--20 days.It is compared from entire production procedure, production cost can be saved more than 60%, had
Good promotional value.
Description of the drawings
Fig. 1 is the method flow diagram that the basic element of cell division provided in an embodiment of the present invention inspires bletilla striata bulb reproduction.
Specific embodiment
In order to make the purpose , technical scheme and advantage of the present invention be clearer, with reference to embodiments, to the present invention
It is further elaborated.It should be appreciated that the specific embodiments described herein are merely illustrative of the present invention, it is not used to
Limit the present invention.
In existing bletilla striata bulb reproduction technique, growth is slow during bulb propagation, bulb consumption is big, planting percent is low.
The present invention carries out the pretreatment of bletilla striata bulb.Using effect hormone carries out seed soaking to bletilla striata bulb, has accelerated
Bletilla striata bulb takes root, sprouts, and improves the speed of growth of bletilla striata bulb.
The present invention carries out seedbed management, accelerates the growth course of bletilla striata bulb.
The present invention is described in detail below in conjunction with the accompanying drawings.
Fig. 1, the method that the basic element of cell division provided in an embodiment of the present invention inspires bletilla striata bulb reproduction, including：
S101：Growth selection is full, healthy bletilla striata bulb without disease pest harm, from bud head rip cutting, bud is made to divide two faces equally,
The bulb of each half requirement at least 8 grams of band or more, to ensure the nutrition supply after germinateing.
S102：It will cut and be put into 500--800 times of carbendazim solution with bud bulb and carry out disinfection processing 5--10 minutes,
It dries spare.
S103：The mixed liquor for the bulb dried being put into 20PPM methyl α-naphthyl acetate+10PPM6- benayl aminopurines is impregnated
20--25 minutes；It dries.
S104：Cultivated the good bulb of chemicals treatment is dried into the seedbed disinfected, the cultivation matrix in seedbed by：It is thin red
The native thin peat 2+ sand 1 of 5+ forms.Planting seeding row spacing is：4 × 6-8 centimetres, the matrix of 0.5-1 centimetres of covering, irrigates on bulb
Water.
S105：On the seedbed transplanted in bulb, water management requirement is：It is not dry do not pour, one irrigates.In the whole of nursery
A growth period temperature requirement is maintained at 20-25 DEG C.
S106：Be spaced 48-72 it is small when, toward seedbed land measure equal to fifteen mu in most parts of the Northeast face on spray a 10PPM6- benayl aminopurine liquid, it is desirable that land measure equal to fifteen mu in most parts of the Northeast face
It squirts, until bud starts to be unearthed.
The present invention obtains 2 parts or more parts of bulb reproduction materials by dividing the terminal bud of the bletilla striata equally, and by disinfection
The probability of bulb introduced disease is reduced after reason.Handled by methyl α-naphthyl acetate, can promote bulb take root it is rapid, grow；It passes through again
The stimulation of 6-benzyl aminopurine is crossed, accelerates the cell division of bulb, improves the speed of growth of bletilla striata bulb, accelerate
Bletilla striata seedling goes out the garden time, has reached the growth time requirement of 10-20 days of bletilla striata bulb reproduction ahead of time.
With reference to specific embodiment, the invention will be further described.
The present invention has selected 50 trident bletilla striata bulbs, each averagely to weigh 26 grams；Terminal bud is divided in rip cutting equally since terminal bud
For 2 half.
It will cut and be put into 500--800 times of carbendazim solution with bud bulb and carry out disinfection processing 10 minutes, dry standby
With.
The mixed liquor for the bulb dried being put into 20PPM methyl α-naphthyl acetate+10PPM6- benayl aminopurines impregnate 25 minutes
Afterwards；It dries.
It is cultivated the good bulb of chemicals treatment is dried into the seedbed disinfected.Planting seeding row spacing is：4 × 6-8 centimetres, kind
0.5--1 centimetres of matrix is covered on ball, is sprinkled profoundly water.
20--25 DEG C is maintained in the entire growth period temperature requirement of nursery.
Interval 2--3 days, toward seedbed land measure equal to fifteen mu in most parts of the Northeast face on spray a 10PPM6- benayl aminopurine liquid, it is desirable that land measure equal to fifteen mu in most parts of the Northeast face squirts i.e.
Can, until bud starts to be unearthed.
It is average to start to emerge in 22--25 or so on seedbed, than compareing time advance 12--20 days or so of averagely emerging.
30 days after emergence, the bletilla striata seedling average height of processing reaches 6--8 centimetres, has reached garden requirement；It is more flat than compareing
Go out garden time advance 15--21 days or so.
The foregoing is merely illustrative of the preferred embodiments of the present invention, is not intended to limit the invention, all essences in the present invention
All any modification, equivalent and improvement made within refreshing and principle etc., should all be included in the protection scope of the present invention.
Claims (6)
1. a kind of method that basic element of cell division inspires bletilla striata bulb reproduction, which is characterized in that the basic element of cell division inspires the bletilla striata
The method of bulb reproduction includes：
The pretreatment of bletilla striata bulb, using effect hormone carry out seed soaking to bletilla striata bulb, accelerate taking root, sprouting for bletilla striata bulb
Hair；
Seedbed management is carried out, promotes the growth course of bletilla striata bulb.
2. the method that the basic element of cell division as described in claim 1 inspires bletilla striata bulb reproduction, which is characterized in that the cell point
The plain method for inspiring bletilla striata bulb reproduction is split to specifically include：
Growth selection is full, healthy bletilla striata bulb without disease pest harm, from bud head rip cutting, bud is made to divide multiple faces equally；
It will cut and be put into 500-800 times of carbendazim solution with bud bulb and carry out disinfection processing, dry spare；
The bulb dried be put into 20PPM methyl α-naphthyl acetate+10PPM6- benayl aminopurines mixed liquor carry out impregnate 20 minutes -25 points
Clock；It dries；
It is cultivated the good bulb of chemicals treatment is dried into the seedbed disinfected；Planting seeding row spacing is：4 6~8 centimetres of cm x,
0.5 centimetre~1 centimetre of matrix is covered on bulb, is sprinkled profoundly water；
On the seedbed transplanted in bulb, water management is carried out；
Be spaced 48 it is small when~72 it is small when, toward seedbed land measure equal to fifteen mu in most parts of the Northeast face on spray a 10PPM6- benayl aminopurine liquid, until bud starts
Until soil.
3. the method that the basic element of cell division as described in claim 1 inspires bletilla striata bulb reproduction, which is characterized in that at the disinfection
It manages as -10 minutes 5 minutes.
4. the method that the basic element of cell division as described in claim 1 inspires bletilla striata bulb reproduction, which is characterized in that the seedbed
Cultivation matrix is made of by mass parts thin 5 parts of laterite, 2 parts of thin peat and husky 1 part.
5. the method that the basic element of cell division as described in claim 1 inspires bletilla striata bulb reproduction, which is characterized in that water management
For：It is not dry do not pour, one irrigates；It it is 20 DEG C~25 DEG C in the entire growth period temperature of nursery.
6. the basic element of cell division for a kind of method that basic element of cell division as described in claim 1 inspires bletilla striata bulb reproduction inspires the bletilla striata
Bulb reproduction system.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201711279408.7A CN108064646A (en) | 2017-12-06 | 2017-12-06 | A kind of method that basic element of cell division inspires bletilla striata bulb reproduction |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201711279408.7A CN108064646A (en) | 2017-12-06 | 2017-12-06 | A kind of method that basic element of cell division inspires bletilla striata bulb reproduction |
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Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103299903A (en) * | 2013-05-23 | 2013-09-18 | 广西壮族自治区中国科学院广西植物研究所 | Efficient bletilla propagation method using pseudobulbs to induce pseudobulbs |
| CN103348913A (en) * | 2013-06-28 | 2013-10-16 | 江苏茅山地道中药材种植有限公司 | Rapid propagation method for bletilla striata |
| CN103749032A (en) * | 2014-01-07 | 2014-04-30 | 陕西师范大学 | Bletilla striata germination accelerating method |
| CN104255496A (en) * | 2014-09-23 | 2015-01-07 | 江苏农林职业技术学院 | Method for immediately propagating bletilla striata test-tube plantlet |
| CN104335903A (en) * | 2014-11-21 | 2015-02-11 | 广西中医药大学 | Method for accelerating quick propagation of rhizoma bletillae |
-
2017
- 2017-12-06 CN CN201711279408.7A patent/CN108064646A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103299903A (en) * | 2013-05-23 | 2013-09-18 | 广西壮族自治区中国科学院广西植物研究所 | Efficient bletilla propagation method using pseudobulbs to induce pseudobulbs |
| CN103348913A (en) * | 2013-06-28 | 2013-10-16 | 江苏茅山地道中药材种植有限公司 | Rapid propagation method for bletilla striata |
| CN103749032A (en) * | 2014-01-07 | 2014-04-30 | 陕西师范大学 | Bletilla striata germination accelerating method |
| CN104255496A (en) * | 2014-09-23 | 2015-01-07 | 江苏农林职业技术学院 | Method for immediately propagating bletilla striata test-tube plantlet |
| CN104335903A (en) * | 2014-11-21 | 2015-02-11 | 广西中医药大学 | Method for accelerating quick propagation of rhizoma bletillae |
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Application publication date: 20180525 |
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