CN108064279B - 噬菌体λ整合酶的突变体 - Google Patents

噬菌体λ整合酶的突变体 Download PDF

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CN108064279B
CN108064279B CN201580054345.8A CN201580054345A CN108064279B CN 108064279 B CN108064279 B CN 108064279B CN 201580054345 A CN201580054345 A CN 201580054345A CN 108064279 B CN108064279 B CN 108064279B
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nucleic acid
sequence
integrase
seq
recombination
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CN108064279A (zh
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F·葛达西
箫嘉玮
P·德罗格
H·玛坎加
S·H·维贾雅钱德拉
S·彼得
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Agency for Science Technology and Research Singapore
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    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/90—Stable introduction of foreign DNA into chromosome
    • C12N15/902—Stable introduction of foreign DNA into chromosome using homologous recombination
    • C12N15/907—Stable introduction of foreign DNA into chromosome using homologous recombination in mammalian cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/22—Ribonucleases [RNase]; Deoxyribonucleases [DNase]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/90—Stable introduction of foreign DNA into chromosome
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1241—Nucleotidyltransferases (2.7.7)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2795/00—Bacteriophages
    • C12N2795/00011—Details
    • C12N2795/10011—Details dsDNA Bacteriophages
    • C12N2795/10311—Siphoviridae
    • C12N2795/10322—New viral proteins or individual genes, new structural or functional aspects of known viral proteins or genes

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  • Health & Medical Sciences (AREA)
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  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
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  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
CN201580054345.8A 2014-08-08 2015-08-11 噬菌体λ整合酶的突变体 Active CN108064279B (zh)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
GBGB1414130.3A GB201414130D0 (en) 2014-08-08 2014-08-08 Mutants of the bacteriophage lambda integrase
GB1414130.3 2014-08-08
PCT/SG2015/050255 WO2016022075A1 (en) 2014-08-08 2015-08-11 Mutants of the bacteriophage lambda integrase

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CN108064279A CN108064279A (zh) 2018-05-22
CN108064279B true CN108064279B (zh) 2022-03-11

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US (2) US10344301B2 (https=)
EP (1) EP3177717A4 (https=)
JP (1) JP6616822B2 (https=)
CN (1) CN108064279B (https=)
GB (1) GB201414130D0 (https=)
SG (2) SG11201700955SA (https=)
WO (1) WO2016022075A1 (https=)

Families Citing this family (14)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB201414130D0 (en) * 2014-08-08 2014-09-24 Agency Science Tech & Res Mutants of the bacteriophage lambda integrase
GB201603577D0 (en) * 2016-03-01 2016-04-13 Oxford Genetics Ltd Promoter
US11078493B2 (en) 2016-06-21 2021-08-03 Nanyang Technological University Site-specific DNA recombination
FI3638776T3 (fi) 2017-06-14 2025-12-09 Univ Dresden Tech Menetelmiä ja välineitä genomien geneettiseen muokkaamiseen hyödyntäen suunnittelu-DNA:ta rekombinoivia entsyymejä
WO2021021964A2 (en) 2019-07-29 2021-02-04 Chondrial Therapeutics, Inc. Methods of quantifying frataxin and frataxin fusion proteins
BR112022001657A2 (pt) * 2019-07-29 2022-03-22 Univ Indiana Trustees Polipeptídeo de fusão, sequência de ácidos nucleicos, cassete de expressão, vetor de expressão, célula hospedeira, composição farmacêutica, e, métodos para tratar a ataxia de friedreich e para tratar uma doença associada à ataxia de friedreich
KR20220108094A (ko) 2019-11-25 2022-08-02 라리마 테라퓨틱스, 인코포레이티드 프라탁신 활성의 정량화 방법
US11976100B2 (en) 2020-04-30 2024-05-07 Larimar Therapeutics, Inc. Methods for treating myelin associated diseases and mitochondria associated diseases
CN116867506A (zh) 2020-12-12 2023-10-10 拉利玛生物医药公司 包括弗里德赖希共济失调蛋白融合蛋白的药物组合物及其使用方法
CN117178056A (zh) * 2021-03-12 2023-12-05 南洋理工大学 用于生产无缝dna载体的方法
WO2023234866A1 (en) * 2022-05-31 2023-12-07 Nanyang Technological University Method for production of a eukaryotic host cell or cell line for lambda-integrase-mediated recombination
CN118546903A (zh) * 2023-02-24 2024-08-27 尧唐(上海)生物科技有限公司 能介导大片段dna整合到哺乳动物基因组的酶及其应用
WO2024181932A1 (en) * 2023-03-02 2024-09-06 Nanyang Technological University Lambda integrase mutants and methods of their use
CN121693564A (zh) 2023-06-26 2026-03-17 夏威夷大学 进化的整合酶和其用于基因组编辑的方法

Citations (1)

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Publication number Priority date Publication date Assignee Title
WO2002097059A2 (en) * 2001-05-30 2002-12-05 Chromos Molecular Systems, Inc. Chromosome-based platforms

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA2522166C (en) 2001-05-30 2011-05-03 Chromos Molecular Systems, Inc. Lambda integrase mutein for use in recombination
US20100192985A1 (en) * 2008-11-11 2010-08-05 Wolfgang Aehle Compositions and methods comprising serine protease variants
SG170652A1 (en) * 2009-11-06 2011-05-30 Agency Science Tech & Res Muteins of the bacteriophage lambda integrases
GB201414130D0 (en) * 2014-08-08 2014-09-24 Agency Science Tech & Res Mutants of the bacteriophage lambda integrase

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2002097059A2 (en) * 2001-05-30 2002-12-05 Chromos Molecular Systems, Inc. Chromosome-based platforms

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
Identifying Determinants of Recombination Specificity: Construction and Characterization of Chimeric Bacteriophage Integrases;Ezra Yagi et al.,;《Journal of Molecular Biology》;19950915;第252卷(第2期);163-177 *
Selection of bacteriophage λ integrases with altered recombination specificity by in vitro compartmentalization;Yvonne Tay et al.,;《Nucleic Acids Research》;20100301;第38卷(第4期);e25 *

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SG11201700955SA (en) 2017-03-30
US20170327847A1 (en) 2017-11-16
GB201414130D0 (en) 2014-09-24
JP2017525355A (ja) 2017-09-07
CN108064279A (zh) 2018-05-22
EP3177717A4 (en) 2017-12-27
JP6616822B2 (ja) 2019-12-04
SG10202005089XA (en) 2020-07-29
EP3177717A1 (en) 2017-06-14
WO2016022075A1 (en) 2016-02-11
US11414681B2 (en) 2022-08-16
US10344301B2 (en) 2019-07-09
US20200063165A1 (en) 2020-02-27

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