CN108043873A - A kind of heavy metal-polluted soil biological fixation agent and its application process - Google Patents
A kind of heavy metal-polluted soil biological fixation agent and its application process Download PDFInfo
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- CN108043873A CN108043873A CN201711423272.2A CN201711423272A CN108043873A CN 108043873 A CN108043873 A CN 108043873A CN 201711423272 A CN201711423272 A CN 201711423272A CN 108043873 A CN108043873 A CN 108043873A
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B09—DISPOSAL OF SOLID WASTE; RECLAMATION OF CONTAMINATED SOIL
- B09C—RECLAMATION OF CONTAMINATED SOIL
- B09C1/00—Reclamation of contaminated soil
- B09C1/10—Reclamation of contaminated soil microbiologically, biologically or by using enzymes
- B09C1/105—Reclamation of contaminated soil microbiologically, biologically or by using enzymes using fungi or plants
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- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B09—DISPOSAL OF SOLID WASTE; RECLAMATION OF CONTAMINATED SOIL
- B09C—RECLAMATION OF CONTAMINATED SOIL
- B09C2101/00—In situ
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Abstract
It is disclosed by the invention to belong to biological fixation agent technical field, it is specially a kind of heavy metal-polluted soil biological fixation agent and its application process, which includes following constituent:Organic fertilizer;Biological carbon;Arbuscular mycorrhizal fungi;Saccharomycete;Algae;Bacillus and clay mineral materials, the application process of the heavy metal-polluted soil biological fixation agent are:Organic fertilizer, biological carbon, arbuscular mycorrhizal fungi, saccharomycete, algae, bacillus and clay mineral materials are uniformly mixed, obtain the heavy metal-polluted soil biological fixation agent, then the heavy metal-polluted soil biological fixation agent is directly applied to contaminated soil, it turns uniformly, fermentation, with height absorption and exchange of particles ability, improve the pH value of soil, indissoluble heavy metal compound precipitation is formed, improves soil property, the formation of soil agreegate can be promoted, improve the stability of soil texture, the invention is of low cost, pollution-free, and environmental risk is small, need not stop to plough.
Description
Technical field
The present invention relates to biological fixation agent technical fields, are specially a kind of heavy metal-polluted soil biological fixation agent and its application side
Method.
Background technology
Heavy metal is a kind of poor mobility, is difficult to degrade and the important soil pollutant with potential hazard, in technology ore deposit
Bed exploitation and smelting, solid waste accumulate and improve agricultural production and apply the processes such as cost, pesticide sludge so that a huge sum of money
Belong to a large amount of in the soil to accumulate, the heavy metal in soil can be absorbed by root system in plant, by the work for inhibiting enzyme
Property, promote ATP degradations, change membrane passage, the damage processes such as hereditary material DNA, influence the growth and reproduction of plant,
Heavy metal is also possible to enter human body by the biological magnification of food chain, threatens health, countries in the world are all in product at present
Explore the measure for solving heavy metal-polluted soil harm in pole.Current physics, chemical heavy metallic prosthetic method there are technology requirement it is high or
Person's financial cost is high, but also can destroy soil microflora.And existing some biological renovation methods presence was repaired
Journey needs stop ploughing or only have the function of the simple function of heavy metal reparation without possessing other.For this purpose, a kind of it is proposed that soil
Earth heavy metal biological fixative and its application process.
The content of the invention
It is an object of the invention to provide a kind of heavy metal-polluted soil biological fixation agent and its application process, to solve the above-mentioned back of the body
Some the existing biological renovation methods proposed in scape technology stop ploughing or only have heavy metal reparation there are repair process needs
Simple function without possessing the problem of other functions.
To achieve the above object, the present invention provides following technical solution:A kind of heavy metal-polluted soil biological fixation agent, the soil
Heavy metal biological fixative includes following constituent:
Organic fertilizer:30-40 parts;Biological carbon:50-60 parts;Arbuscular mycorrhizal fungi:10-14 parts;Saccharomycete:4-6 parts;Algae:
10-16 parts;Bacillus:8-10 parts and clay mineral materials:60-70 parts.
Preferably, the main component of the organic fertilizer is animal wastes.
Preferably, the biological carbon is stalk, fallen leaves and shell biological carbon.
Preferably, the algae is at least one of Synechococcus and green alga.
Preferably, the viable count of the bacillus is (0.5-5) × 108CFU/g.
Preferably, the culture medium of the bacillus mainly includes following constituent:Corn pulp, glucose, phosphoric acid hydrogen
Dipotassium, ammonium sulfate, magnesium sulfate, yeast, cream and peptone.
A kind of application process of heavy metal-polluted soil biological fixation agent, the application process of the heavy metal-polluted soil biological fixation agent
For:Organic fertilizer, biological carbon, arbuscular mycorrhizal fungi, saccharomycete, algae, bacillus and clay mineral materials are uniformly mixed, are obtained
To the heavy metal-polluted soil biological fixation agent, the heavy metal-polluted soil biological fixation agent is then directly applied to contaminated soil, is turned over
It mixes uniformly, fermentation.
Preferably, the mass ratio of the heavy metal-polluted soil biological fixation agent and contaminated soil is (0.08-0.18):100.
Preferably, the fermentation time is 4-5 days, and fermentation temperature is 18-28 degrees Celsius.
Compared with prior art, the beneficial effects of the invention are as follows:A kind of heavy metal-polluted soil biological fixation that the invention proposes
Agent and its application process have high absorption and exchange of particles ability, improve the pH value of soil, form indissoluble heavy metal compound and sink
It forms sediment, improves soil property, the formation of soil agreegate can be promoted, improve the stability of soil texture, the invention is of low cost, no dirt
Dye, environmental risk is small, need not stop to plough.
Specific embodiment
The technical solution in the embodiment of the present invention will be clearly and completely described below, it is clear that described implementation
Example is only part of the embodiment of the present invention, instead of all the embodiments.Based on the embodiments of the present invention, this field is common
Technical staff's all other embodiments obtained without making creative work belong to the model that the present invention protects
It encloses.
The present invention provides a kind of heavy metal-polluted soil biological fixation agent, which includes following composition
Ingredient:
Organic fertilizer:30-40 parts, the main component of the organic fertilizer is animal wastes;Biological carbon:50-60 parts, the biology
Carbon is stalk, fallen leaves and shell biological carbon;Arbuscular mycorrhizal fungi:10-14 parts;Saccharomycete:4-6 parts;Algae:It is 10-16 parts, described
Algae is at least one of Synechococcus and green alga;Bacillus:8-10 parts, the viable count of the bacillus is (0.5-5)
× 108CFU/g, the culture medium of the bacillus mainly include following constituent:Corn pulp, glucose, dipotassium hydrogen phosphate,
Ammonium sulfate, magnesium sulfate, yeast, cream and peptone;Clay mineral materials:60-70 parts.
The present invention also provides a kind of application processes of heavy metal-polluted soil biological fixation agent:
Embodiment 1
The application process of the heavy metal-polluted soil biological fixation agent is:By organic fertilizer, biological carbon, arbuscular mycorrhizal fungi, yeast
Bacterium, algae, bacillus and clay mineral materials are uniformly mixed, and are obtained the heavy metal-polluted soil biological fixation agent, then should
Heavy metal-polluted soil biological fixation agent is directly applied to contaminated soil, turns uniformly, fermentation, and the heavy metal-polluted soil biology is solid
The mass ratio for determining agent and contaminated soil is 0.08:100, the fermentation time is 4 days, and fermentation temperature is 18 degrees Celsius.
In the present embodiment, organic fertilizer:39 parts, the main component of the organic fertilizer is animal wastes;Biological carbon:58 parts,
The biological carbon is stalk, fallen leaves and shell biological carbon;Arbuscular mycorrhizal fungi:14 parts;Saccharomycete:4 parts;Algae:It is 16 parts, described
Algae is at least one of Synechococcus and green alga;Bacillus:8 parts, the viable count of the bacillus for 0.5 ×
108CFU/g, the culture medium of the bacillus mainly include following constituent:Corn pulp, glucose, dipotassium hydrogen phosphate, sulphur
Sour ammonium, magnesium sulfate, yeast, cream and peptone;Clay mineral materials:60 parts.
Embodiment 2
The application process of the heavy metal-polluted soil biological fixation agent is:By organic fertilizer, biological carbon, arbuscular mycorrhizal fungi, yeast
Bacterium, algae, bacillus and clay mineral materials are uniformly mixed, and are obtained the heavy metal-polluted soil biological fixation agent, then should
Heavy metal-polluted soil biological fixation agent is directly applied to contaminated soil, turns uniformly, fermentation, and the heavy metal-polluted soil biology is solid
The mass ratio for determining agent and contaminated soil is 0.18:100, the fermentation time is 5 days, and fermentation temperature is 28 degrees Celsius.
In the present embodiment, organic fertilizer:35 parts, the main component of the organic fertilizer is animal wastes;Biological carbon:58 parts,
The biological carbon is stalk, fallen leaves and shell biological carbon;Arbuscular mycorrhizal fungi:11 parts;Saccharomycete:4 parts;Algae:It is 11 parts, described
Algae is at least one of Synechococcus and green alga;Bacillus:10 parts, the viable count of the bacillus is 5 × 108CFU/
G, the culture medium of the bacillus mainly include following constituent:Corn pulp, glucose, dipotassium hydrogen phosphate, ammonium sulfate, sulphur
Sour magnesium, yeast, cream and peptone;Clay mineral materials:60 parts.
Embodiment 3
The application process of the heavy metal-polluted soil biological fixation agent is:By organic fertilizer, biological carbon, arbuscular mycorrhizal fungi, yeast
Bacterium, algae, bacillus and clay mineral materials are uniformly mixed, and are obtained the heavy metal-polluted soil biological fixation agent, then should
Heavy metal-polluted soil biological fixation agent is directly applied to contaminated soil, turns uniformly, fermentation, and the heavy metal-polluted soil biology is solid
The mass ratio for determining agent and contaminated soil is 0.13:100, the fermentation time is 4.5 days, and fermentation temperature is 23 degrees Celsius.
In the present embodiment, organic fertilizer:35 parts, the main component of the organic fertilizer is animal wastes;Biological carbon:55 parts,
The biological carbon is stalk, fallen leaves and shell biological carbon;Arbuscular mycorrhizal fungi:12 parts;Saccharomycete:5 parts;Algae:It is 13 parts, described
Algae is at least one of Synechococcus and green alga;Bacillus:9 parts, the viable count of the bacillus for 0.75 ×
108CFU/g, the culture medium of the bacillus mainly include following constituent:Corn pulp, glucose, dipotassium hydrogen phosphate, sulphur
Sour ammonium, magnesium sulfate, yeast, cream and peptone;Clay mineral materials:65 parts.
It although an embodiment of the present invention has been shown and described, for the ordinary skill in the art, can be with
Understanding without departing from the principles and spirit of the present invention can carry out these embodiments a variety of variations, modification, replace
And modification, the scope of the present invention is defined by the appended.
Claims (9)
1. a kind of heavy metal-polluted soil biological fixation agent, it is characterised in that:The heavy metal-polluted soil biological fixation agent includes following composition
Ingredient:
Organic fertilizer:30-40 parts;Biological carbon:50-60 parts;Arbuscular mycorrhizal fungi:10-14 parts;Saccharomycete:4-6 parts;Algae:10-
16 parts;Bacillus:8-10 parts and clay mineral materials:60-70 parts.
2. a kind of heavy metal-polluted soil biological fixation agent according to claim 1, it is characterised in that:The organic fertilizer it is main
Ingredient is animal wastes.
3. a kind of heavy metal-polluted soil biological fixation agent according to claim 1, it is characterised in that:The biological carbon is straw
Stalk, fallen leaves and shell biological carbon.
4. a kind of heavy metal-polluted soil biological fixation agent according to claim 1, it is characterised in that:The algae is Synechococcus
At least one of with green alga.
5. a kind of heavy metal-polluted soil biological fixation agent according to claim 1, it is characterised in that:The work of the bacillus
Bacterium number is (0.5-5) × 108CFU/g.
6. a kind of heavy metal-polluted soil biological fixation agent according to claim 1, it is characterised in that:The training of the bacillus
Foster base mainly includes following constituent:Corn pulp, glucose, dipotassium hydrogen phosphate, ammonium sulfate, magnesium sulfate, yeast, cream and albumen
Peptone.
7. a kind of application process of heavy metal-polluted soil biological fixation agent, it is characterised in that:The heavy metal-polluted soil biological fixation agent
Application process is:Organic fertilizer, biological carbon, arbuscular mycorrhizal fungi, saccharomycete, algae, bacillus and clay mineral materials are mixed
It closes uniformly, obtains the heavy metal-polluted soil biological fixation agent, the heavy metal-polluted soil biological fixation agent is then directly applied to dirt
Soil is contaminated, is turned uniformly, fermentation.
8. a kind of application process of heavy metal-polluted soil biological fixation agent according to claim 7, it is characterised in that:The soil
The mass ratio of earth heavy metal biological fixative and contaminated soil is (0.08-0.18):100.
9. a kind of application process of heavy metal-polluted soil biological fixation agent according to claim 7, it is characterised in that:The hair
The ferment time is 4-5 days, and fermentation temperature is 18-28 degrees Celsius.
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Cited By (6)
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CN108636356A (en) * | 2018-06-05 | 2018-10-12 | 吉林农业大学 | A kind of cornstalk biological charcoal and restorative procedure that can repair nitrogen phosphorus pollution of area source |
CN108723082A (en) * | 2018-06-22 | 2018-11-02 | 煜环环境科技有限公司 | A kind of load fixed combined contamination soil chemical oxidation restorative procedure |
CN109679663A (en) * | 2018-12-29 | 2019-04-26 | 云南农业大学 | A kind of soil in protected field modifying agent and its method of administration |
CN112457857A (en) * | 2020-10-31 | 2021-03-09 | 兰州资源环境职业技术学院 | Soil conditioner for removing heavy metals in coal mining area |
CN114011382A (en) * | 2021-10-22 | 2022-02-08 | 昆明理工大学 | Preparation method and application of organic-inorganic compound |
CN114075438A (en) * | 2021-12-02 | 2022-02-22 | 上海康恒环境修复有限公司 | Composite biological agent for repairing heavy metal contaminated soil and preparation method and application thereof |
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Cited By (7)
Publication number | Priority date | Publication date | Assignee | Title |
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CN108636356A (en) * | 2018-06-05 | 2018-10-12 | 吉林农业大学 | A kind of cornstalk biological charcoal and restorative procedure that can repair nitrogen phosphorus pollution of area source |
CN108723082A (en) * | 2018-06-22 | 2018-11-02 | 煜环环境科技有限公司 | A kind of load fixed combined contamination soil chemical oxidation restorative procedure |
CN108723082B (en) * | 2018-06-22 | 2020-10-20 | 煜环环境科技有限公司 | Chemical oxidation remediation method for load-immobilized composite contaminated soil |
CN109679663A (en) * | 2018-12-29 | 2019-04-26 | 云南农业大学 | A kind of soil in protected field modifying agent and its method of administration |
CN112457857A (en) * | 2020-10-31 | 2021-03-09 | 兰州资源环境职业技术学院 | Soil conditioner for removing heavy metals in coal mining area |
CN114011382A (en) * | 2021-10-22 | 2022-02-08 | 昆明理工大学 | Preparation method and application of organic-inorganic compound |
CN114075438A (en) * | 2021-12-02 | 2022-02-22 | 上海康恒环境修复有限公司 | Composite biological agent for repairing heavy metal contaminated soil and preparation method and application thereof |
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Application publication date: 20180518 |