CN108040459B - 将药剂递送至产卵水生动物的卵中的方法 - Google Patents

将药剂递送至产卵水生动物的卵中的方法 Download PDF

Info

Publication number
CN108040459B
CN108040459B CN201680028215.1A CN201680028215A CN108040459B CN 108040459 B CN108040459 B CN 108040459B CN 201680028215 A CN201680028215 A CN 201680028215A CN 108040459 B CN108040459 B CN 108040459B
Authority
CN
China
Prior art keywords
guanidine
eggs
fish
egg
containing compound
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
CN201680028215.1A
Other languages
English (en)
Chinese (zh)
Other versions
CN108040459A (zh
Inventor
翁天佐
约纳坦·祖海尔
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
University of Maryland Baltimore County UMBC
Original Assignee
University of Maryland Baltimore County UMBC
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by University of Maryland Baltimore County UMBC filed Critical University of Maryland Baltimore County UMBC
Publication of CN108040459A publication Critical patent/CN108040459A/zh
Application granted granted Critical
Publication of CN108040459B publication Critical patent/CN108040459B/zh
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Images

Classifications

    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
    • A01K67/027—New or modified breeds of vertebrates
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
    • A01K67/027—New or modified breeds of vertebrates
    • A01K67/0275—Genetically modified vertebrates, e.g. transgenic
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2207/00—Modified animals
    • A01K2207/05—Animals modified by non-integrating nucleic acids, e.g. antisense, RNAi, morpholino, episomal vector, for non-therapeutic purpose
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2207/00—Modified animals
    • A01K2207/20—Animals treated with compounds which are neither proteins nor nucleic acids
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2227/00—Animals characterised by species
    • A01K2227/40—Fish
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2267/00—Animals characterised by purpose
    • A01K2267/02—Animal zootechnically ameliorated
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2267/00—Animals characterised by purpose
    • A01K2267/03—Animal model, e.g. for test or diseases
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/10—Type of nucleic acid
    • C12N2310/11—Antisense
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/30—Chemical structure
    • C12N2310/32—Chemical structure of the sugar
    • C12N2310/323—Chemical structure of the sugar modified ring structure
    • C12N2310/3233—Morpholino-type ring
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/30—Chemical structure
    • C12N2310/35—Nature of the modification
    • C12N2310/351—Conjugate
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2320/00—Applications; Uses
    • C12N2320/30—Special therapeutic applications
    • C12N2320/32—Special delivery means, e.g. tissue-specific
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
    • Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
    • Y02A40/81—Aquaculture, e.g. of fish
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A90/00—Technologies having an indirect contribution to adaptation to climate change
    • Y02A90/40—Monitoring or fighting invasive species

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Chemical & Material Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Environmental Sciences (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • Animal Behavior & Ethology (AREA)
  • Animal Husbandry (AREA)
  • Biodiversity & Conservation Biology (AREA)
  • Veterinary Medicine (AREA)
  • Farming Of Fish And Shellfish (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Enzymes And Modification Thereof (AREA)
CN201680028215.1A 2015-05-19 2016-05-17 将药剂递送至产卵水生动物的卵中的方法 Active CN108040459B (zh)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US201562163511P 2015-05-19 2015-05-19
US62/163,511 2015-05-19
US201662274958P 2016-01-05 2016-01-05
US62/274,958 2016-01-05
PCT/US2016/032873 WO2016187198A2 (en) 2015-05-19 2016-05-17 Methods of agent delivery into eggs and embryos of egg-producing aquatic animals for drug screening, agent toxicity assay and production of infertile fish

Publications (2)

Publication Number Publication Date
CN108040459A CN108040459A (zh) 2018-05-15
CN108040459B true CN108040459B (zh) 2021-09-21

Family

ID=56084421

Family Applications (1)

Application Number Title Priority Date Filing Date
CN201680028215.1A Active CN108040459B (zh) 2015-05-19 2016-05-17 将药剂递送至产卵水生动物的卵中的方法

Country Status (13)

Country Link
US (1) US10709119B2 (enExample)
EP (1) EP3297429A2 (enExample)
JP (1) JP6756960B2 (enExample)
CN (1) CN108040459B (enExample)
AU (1) AU2016265929B2 (enExample)
BR (1) BR112017024662A2 (enExample)
CA (1) CA2984644A1 (enExample)
CL (1) CL2017002864A1 (enExample)
DK (1) DK180094B1 (enExample)
IL (1) IL255338B (enExample)
MX (1) MX386910B (enExample)
RU (1) RU2741645C2 (enExample)
WO (1) WO2016187198A2 (enExample)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2015073819A1 (en) 2013-11-15 2015-05-21 University Of Maryland Baltimore County Method of producing infertile fish and egg-producing aquatic animals and of delivering compounds into eggs and embryos
PL3723479T3 (pl) * 2017-12-15 2024-10-07 Acd Pharmaceuticals As Sposoby produkcji sterylnych ryb
WO2020125848A1 (de) * 2018-12-17 2020-06-25 Alfred-Wegener-Institut, Helmholtz-Zentrum für Polar- und Meeresforschung Verfahren, das der herstellung von kaviar oder einem kaviarähnlichem produkt aus lebenden, reifen eiern von fischen oder krebstieren dient, und solche produkte

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101553210A (zh) * 2006-09-20 2009-10-07 奈克斯特安全有限公司 经吸入输送药物的方法和系统
EP2535404A1 (en) * 2010-02-09 2012-12-19 National University Corporation Hokkaido University Method for acquiring genetically identical gamete from lethal fish haploid-derived germ cell via germ line chimera
CN103957942A (zh) * 2011-10-11 2014-07-30 皇家兽医学院 方法
CN105764333A (zh) * 2013-11-15 2016-07-13 马里兰大学巴尔的摩县 生产不育的鱼和产卵水生动物及递送化合物至卵和胚胎中的方法

Family Cites Families (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7115794B2 (en) * 2003-05-19 2006-10-03 University Of Hawaii Method for direct nucleic acid uptake
US7194978B2 (en) 2003-08-22 2007-03-27 University Of Maryland Biotechnology Institute Inducing sterility in fish by disrupting the development of the GnRH system
CN1294808C (zh) 2003-09-26 2007-01-17 中国科学院水生生物研究所 不育转基因鱼的制备方法
CN1274822C (zh) 2004-02-19 2006-09-13 武汉康祥科技发展有限公司 活体化学诱导鱼类雌核发育方法
CN103898161A (zh) 2007-01-26 2014-07-02 辛那杰瓦生物制药股份有限公司 在禽类中进行转基因表达
US7935816B2 (en) 2007-10-25 2011-05-03 Gene Tools, Llc Molecular transporter compositions comprising dendrimeric oligoguanidine with a triazine core that facilitate delivery into cells in vivo
US8383880B2 (en) 2009-02-18 2013-02-26 Academia Sinica Infertility control of genetically modified fish
WO2011018798A2 (en) * 2009-08-14 2011-02-17 Indian Association For The Cultivation Of Science Morpholino-based antisense agent
CA2825994A1 (en) 2011-02-01 2012-08-09 Ten-Tsao WONG Efficient sterilization of fish by disruption of germ cell development
RU2504389C2 (ru) * 2012-02-16 2014-01-20 Федеральное государственное бюджетное учреждение науки Институт биохимии и физиологии микроорганизмов им. Г.К. Скрябина Российской академии наук (ИБФМ РАН) СПОСОБ ПОРОФОРМИРОВАНИЯ В МЕМБРАНАХ КЛЕТОК ПЕЧЕНИ С ПОМОЩЬЮ ОБРАБОТКИ ИХ ГЕМОЛИЗИНОМ II Bacillus cereus
US9538734B2 (en) 2013-03-12 2017-01-10 The General Hospital Corporation Germ cell ablation compounds and uses thereof

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101553210A (zh) * 2006-09-20 2009-10-07 奈克斯特安全有限公司 经吸入输送药物的方法和系统
EP2535404A1 (en) * 2010-02-09 2012-12-19 National University Corporation Hokkaido University Method for acquiring genetically identical gamete from lethal fish haploid-derived germ cell via germ line chimera
CN103957942A (zh) * 2011-10-11 2014-07-30 皇家兽医学院 方法
CN105764333A (zh) * 2013-11-15 2016-07-13 马里兰大学巴尔的摩县 生产不育的鱼和产卵水生动物及递送化合物至卵和胚胎中的方法

Also Published As

Publication number Publication date
RU2741645C2 (ru) 2021-01-28
US20180295817A1 (en) 2018-10-18
CL2017002864A1 (es) 2018-05-18
MX2017014856A (es) 2018-04-20
CA2984644A1 (en) 2016-11-24
DK180094B1 (en) 2020-04-27
AU2016265929A1 (en) 2017-11-09
DK201770877A1 (en) 2017-11-27
RU2017142635A3 (enExample) 2019-12-16
NZ737306A (en) 2025-02-28
JP6756960B2 (ja) 2020-09-16
MX386910B (es) 2025-03-19
JP2018515116A (ja) 2018-06-14
AU2016265929B2 (en) 2022-03-31
BR112017024662A2 (pt) 2018-09-11
WO2016187198A3 (en) 2017-01-12
IL255338B (en) 2022-05-01
WO2016187198A2 (en) 2016-11-24
EP3297429A2 (en) 2018-03-28
US10709119B2 (en) 2020-07-14
CN108040459A (zh) 2018-05-15
IL255338A0 (en) 2017-12-31
RU2017142635A (ru) 2019-06-20

Similar Documents

Publication Publication Date Title
CN105764333B (zh) 生产不育的鱼和产卵水生动物及递送化合物至卵和胚胎中的方法
KR101797784B1 (ko) 동물에서 모계 유도성 불임
Hattori et al. Surrogate production of Salmo salar oocytes and sperm in triploid Oncorhynchus mykiss by germ cell transplantation technology
CN108040459B (zh) 将药剂递送至产卵水生动物的卵中的方法
Cai et al. Molecular characterization of Pacific white shrimp (Litopenaeus vannamei) sodium bicarbonate cotransporter (NBC) and its role in response to pH stress
US20050132969A1 (en) Inducing sterility in fish by disrupting the development of the GnRH system
Parenrengi et al. Enhancement of tiger shrimp Penaeus monodon resistance to white spot syndrome virus by overexpression of antiviral gene
Chiu Liao et al. Timing and factors affecting cannibalism in red drum, Sciaenops ocellatus, larvae in captivity
Zhou et al. Both of marine fish species Oryzias melastigma and Pagrus major all failing in early localization at embryo stage by vasa RNA
Xu Developing Technologies to Produce Reproductively Sterile Finfish and Oysters, and Characterizing Changes of Gene Expression in the Pituitary of Sterile Fish by Single-Cell RNA Sequencing
BR112016011080B1 (pt) Método para a produção de peixe reprodutivamente estéril e composição para tratamento de ovo(s) de peixe para gerar peixe produzido a partir do(s) mesmo(s), reprodutivamente estéril
Xu et al. Morpholino Oligomer Delivery by Bath Immersion Enables Reverse Genetic Studies in the Early Development of Eastern Oysters (Crassostrea Virginica)
Linhartová et al. Crispr-Cas13d Mediated Germ Cell Ablation in Sterlet Sturgeon: Advancing Surrogate Reproduction Technologies for Endangered Species
NZ737306B2 (en) Methods of agent delivery into eggs of egg-producing aquatic animals
Bian et al. Cloning of Heat Shock Protein 90 from the Marsupenaeus Japonicus and its Anti-Apoptotic Role in Low Temperature Stress

Legal Events

Date Code Title Description
PB01 Publication
PB01 Publication
SE01 Entry into force of request for substantive examination
SE01 Entry into force of request for substantive examination
GR01 Patent grant
GR01 Patent grant