CN108030755A - It is a kind of to dispel blackhead skin care item and preparation method thereof containing Chinese wolfberry fruit dry cell extract - Google Patents
It is a kind of to dispel blackhead skin care item and preparation method thereof containing Chinese wolfberry fruit dry cell extract Download PDFInfo
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Abstract
The invention discloses dispel blackhead skin care item containing Chinese wolfberry fruit dry cell extract,Include the component of following parts by weight,2~8 parts of Chinese wolfberry fruit dry cell extract,5~12 parts of Herba Dendrobii extract,10~15 parts of bletilla striata extract,6~12 parts of coptis extract,The present invention, which provides the extracted obtained Chinese medical extract of Chinese medicine composition, has good anti-inflammatory,Adjust skin oil and fat secretion,The effect of blackhead can be dispelled,And preparation method is easy,The skin care formulation being prepared using it,Products obtained therefrom is green safe,Effect is notable,Easily absorb,There is good compatibility to human body using the stem cell of matrimony vine,It is easier to be absorbed by the body,Growth to Skin Cell has good facilitation,It can accelerate the metabolism of cell,Strengthen the vigor of Skin Cell,Add Herba Dendrobii extract at the same time,Bletilla striata extract,Coptis extract acts synergistically with Chinese wolfberry fruit dry cell extract,Play the synergy synergistic effect for blackhead of dispelling,Positive effect is higher than individually addition one of which or two kinds of extracts.
Description
Technical field
The present invention relates to cosmetic technical field, it is specifically related to a kind of dispel blackhead skin care containing Chinese wolfberry fruit dry cell extract
Product and preparation method thereof.
Background technology
It is everyone nature to like to be beautiful, with the raising of people's living standard, need of the people in terms of material is met
After asking, the beauty and nursing problem of appearance are also increasingly paid attention to.
Blackhead is also known as blackhead, and for open comedones, (top layer for blocking the sebum of pore is directly exposed to outside, with sky
Dust contact in gas, air).Be skin oil and fat it is aerial oxidation and cause, smelly nigrescence, blackhead is common in green grass or young crops
Spring budding teenager, it is most to be apt to occur in face, the small blackhead of shirtfront and back, especially nose, it is characterized in that obvious expand
Stain in big pore, blacks shaped like small worm, top after extrusion.Blackhead is mainly to be made of sebum, cell bits and bacterium
A kind of " bolt " sample thing, is blocked at hair follicle opening and is formed.Plus the dust in air, dirt and oxidation, connect it
A gradual blackening of air is touched, so obtaining so one less graceful title -- blackhead.Blackhead is hardened fat obstruction
Thing, typically occurs in the positions such as forehead, the nose of face, when grease gland swashs by intermuscular needling, pore full of unnecessary grease and
When causing obstruction, in nose and its peripheral part, greasy sensation is often had.These greases eventually harden, after oxidized
As the dot of black, these dots are exactly the grease obturator referred to as blackhead.
Typically now by adding abrasive particle, will be abraded exposed to the blackhead of skin surface, this method effect is poor, can not
Achieve the purpose that to dredge pore.More thoroughly pore cleanser method is " expansion abduction ", i.e., first makes skin with product or steam
Pore expands, and then clings blackhead with suction film and tears, then receives thin water pore refining using pore, reach removal blackhead, dredge
The purpose of logical pore.But this method generally requires specialist cosmetology personage operation, but it is in great pain, and program is cumbersome.
Plant stem cell (plant stem cell) is undifferentiated cell, has very strong self-renewing and persistently divides
Split ability.Research shows that the skin care item containing stem cell can stimulate cell turnover, promotes collagen secretion.Using dry thin
The effect of born of the same parents is more rapidly more effective than general skin care item.Chinese patent CN 102670438B disclose a kind of anti-aging beauty-care shield
Skin product, containing plant stem cell active matter 0.01~3wt% of 0.001~3wt%, Dragosine carnosine, the plant does carefully
Cytoactive thing is PhytocelltecTMMalusDomestica apples stem cell, PhytocelltecTMSolarVitis grapes
Stem cell, PhytocelltecTMAlpRose rose leafs stem cell, PhytocelltecTM Arga n nuts stem cell,
At least one of RegenistemRiceAp red rice stem cells.The cosmetics and skincare product has improvement skin quality, improves skin elasticity
Effect.
Do not occur also on the market at present using report of the plant stem cell for blackhead of dispelling.
The content of the invention
There is provided it is an object of the invention to overcome the above problem using Chinese medicine as main Effect raw material, reasonable group of each component
Close, cooperate with shared, adjust skin oil and fat secretion, the skin care item of blackhead can be dispelled.
To achieve the above object, the present invention adopts the technical scheme that one kind is dispelled blackhead skin care item, including following parts by weight
Component, 2~8 parts of Chinese wolfberry fruit dry cell extract, 5~12 parts of Herba Dendrobii extract, 10~15 parts of bletilla striata extract, coptis extract
6~12 parts.
Preferably, the component of following parts by weight, 3~7.5 parts of Chinese wolfberry fruit dry cell extract, Herba Dendrobii extract 6~11 are included
Part, 10~14 parts of bletilla striata extract, 7.5~11 parts of coptis extract.
Preferably, the component of following parts by weight, 4~6.5 parts of Chinese wolfberry fruit dry cell extract, Herba Dendrobii extract 8~10 are included
Part, 12~14 parts of bletilla striata extract, 7.5~10.5 parts of coptis extract.
Preferably, the component of following parts by weight is included, 5~6.5 parts of Chinese wolfberry fruit dry cell extract, Herba Dendrobii extract 7.5~
10 parts, 12.5~14 parts of bletilla striata extract, 8.5~10.5 parts of coptis extract.
Preferably, the component of following parts by weight, 5~6 parts of Chinese wolfberry fruit dry cell extract, Herba Dendrobii extract 7.5~9.5 are included
Part, 12.5~13 parts of bletilla striata extract, 8.5~10 parts of coptis extract.
Preferably, the component of following parts by weight, 5 parts of matrimony vine stem cell, 7.5 parts of Herba Dendrobii extract, bletilla striata extract are included
13 parts, 8.5 parts of coptis extract.
Preferably, the component of following parts by weight, 6 parts of matrimony vine stem cell, 10 parts of Herba Dendrobii extract, bletilla striata extract are included
12.5 parts.
Preferably, the component of following parts by weight, 6.5 parts of matrimony vine stem cell, 9.5 parts of Herba Dendrobii extract, bletilla striata extraction are included
14 parts of thing, 10.5 parts of coptis extract.
Preferably, the component of following parts by weight is further included, chelating agent is 0.01-0.1 parts, 5-18 parts of moisturizer, preservative
0.3-0.6 parts, 80~150 parts of deionized water；
The moisturizer is Sodium Hyaluronate, a kind of in glycerine, propane diols, Fructus Hordei Germinatus oligose base glucosides；
The preservative is methyl hydroxybenzoate, Phenoxyethanol/at least one of Sensiva SC50 or Kathon CG；
The chelating agent is a kind of in EDTA- disodiums or Etidronic Acid.
One kind is dispelled blackhead skin care item preparation method, is comprised the following steps：
1) preparation of the Chinese wolfberry fruit dry cell extract：A) after the new branch sterilization of matrimony vine, xylem and marrow being removed, inoculation
In inducing culture, inducing culture obtains cambial cell；B) cambial cell through culture medium through subculture, access Multiplying culture
Base, shaking table culture obtain unicellular；C) the unicellular proliferated culture medium that is inoculated in is cultivated through expanding, and obtains matrimony vine stem cell；
D) matrimony vine stem cell after the amplification it is chilled it is broken after concentrate, that is, harvest Chinese wolfberry fruit dry cell extract；
2) by the Chinese wolfberry fruit dry cell extract, Herba Dendrobii extract, bletilla striata extract, coptis extract, moisturizer, anti-corrosion
Agent, carbomer, chelating agent, deionized water are added in pot, are heated to 85~90 DEG C, stirring and dissolving, the cooling of open cycle water, is protected
Hold stirring and vacuumize defoaming；When continuing to be cooled to 36 DEG C, discharging, gets product.
Blackhead skin care item main component of dispelling used in the present invention and effect are as follows：
Matrimony vine：Required nutriment containing the healthy eye such as abundant carrotene, multivitamin and calcium, iron, therefore have
The work(of improving eyesight, is commonly called as " bright eye ".Thing duskization and yctalopia are regarded caused by ancient Chinese medicine doctor treatment deficiency of liver-blood, kidney deficiency, often
Often use matrimony vine.Also, matrimony vine is from the Book of Songs " combining in luxuriant Qi ", just it is used for medicine, so far the existing history in more than 3000 years, skin
Skin aging is mainly due to caused by free-radical oxidation, and polysaccharides contained in matrimony vine, mono- carrotene of B, flavonoids are all
The antioxidant of strength, along with the trace elements of selenium and the synergistic effect of vitamin E contained by matrimony vine, constitutes powerful antioxygen
Change army；In addition, vitamin A can maintain the growth and differentiation of epithelial tissue, dry skin and follicular keratosis are prevented, so as to play
The effect of beautifying face and moistering lotion, moisturizing.
It is raw material that the present invention, which selects the stem cell of matrimony vine, and plant stem cell includes on development of plants and grows all
Formula, possesses the cell of eternal life power, is the root (origin) of plant life power.Plant stem cell, which is present in, to be referred to as point
In the special tectonic of raw tissue, there is very surprising power of regeneration.These allow plant constantly to be grown between centuries,
And generate brand-new organ.Plant stem cell is the primary raw material and source of skin progenitor cell metabolism, can quickly be activated not
Dormancy stem cell, promotes stem cell self-regeneration renewal, and the first viability of reduction epidermal stem cells, makes Skin Cell newborn in a steady stream, from
And reach lifting, firm skin, desalinate the anti-aging effects of eye wrinkle-removing, while can be easier to be absorbed by the skin the battalion of plant
Form point；Accelerate human metabolism.
The stem of noble dendrobium：The stem of noble dendrobium can significantly improve superoxide dismutase (SOD) level, reduce lipid peroxide (LPO), adjust brain
Monoaminergic transmitters are horizontal, suppress similar monoamine oxidase (MAO), play the role of anti-aging.Received much concern in recent years
Dendrobium polysaccharide also there are the multiple efficacies such as significant immune-enhancing activity and anti-aging, radioresistance.Such as Capsule Dendrobii, stone
Dry measure used in former times dissipates, clearing is peaceful, beneficial intestines soup, noble dendrobium stem distillate, stem of noble dendrobium essence, yin-nourishing oral liquid and stem of noble dendrobium piece etc..
The bletilla striata：Containing raw materials such as abundant starch, glucose, volatile oil and mucilaginous substances in the bletilla striata, there is whitening spot-removing, receive
Hemostasis, the function and effect of detumescence and promoting granulation are held back,《Haigoushen》On have the saying of " control sore on face, make us flesh cunning ",《Compendium of Materia Medica》
It is upper to describe its " washing one's face black, nti-freckle ".
The coptis：(root) is bitter, trembles with fear, nontoxic.The thoughts of returning home, spleen, stomach, liver, courage, large intestine channel.Heat-clearing and damp-drying drug, purging intense heat and detonicating.For wet
Hot feeling of fullness, vomits acid regurgitation, and rush down dysentery, jaundice, unconsciousness due to high fever, heart-fire hyperactivity, dysphoria and insomnia, blood-head tells nosebleed, hot eyes, toothache, quenches one's thirst,
Carbuncle swells furunculosis；Eczema, wet sore are controlled outside, duct is suppurated.It is burning hot that prepared RHIZOMA COPTIDIS with vino is apt to the clear part of the body cavity above the diaphragm housing the heart and lungs.For hot eyes, aphtha.Prepared RHIZOMA COPTIDIS with rhizoma zingiberis recens juice clearing stomach and
Stomach preventing or arresting vomiting.Mutually tied for fever and chills, damp and hot middle resistance, feeling of fullness vomiting.Cornel coptis is relaxed liver anti-vomiting.For liver-stomach disharmony, vomiting gulps down
Acid.
Beneficial effects of the present invention：The present invention, which provides the extracted obtained Chinese medical extract of Chinese medicine composition, to be had well
Anti-inflammatory, adjust skin oil and fat secretion, can dispel the effect of blackhead, and preparation method is easy, the skin care being prepared using it
Preparation, products obtained therefrom is green safe, and effect is notable, easily absorbs, and it is component that the present invention, which selects Chinese wolfberry fruit dry cell extract, utilizes Chinese holly
The active ingredient of Qi, can activating cell, remove free radical, firm skin effect, has human body using the stem cell of matrimony vine
Good compatibility, it is easier to be absorbed by the body, the growth to Skin Cell has good facilitation, can accelerate cell
Metabolism, strengthen the vigor of Skin Cell, while add Herba Dendrobii extract, bletilla striata extract, coptis extract and matrimony vine
Stem cell extract acts synergistically, and plays the synergy synergistic effect for blackhead of dispelling, under plurality of Chinese extract synergistic effect,
Product can be obviously improved skin quality, improve skin elasticity, positive effect is higher than individually addition one of which or two kinds of extracts.
Embodiment
To better illustrate the object, technical solutions and advantages of the present invention, below in conjunction with specific embodiment to the present invention
It is described further.
It is a kind of to dispel blackhead skin care item containing Chinese wolfberry fruit dry cell extract, include the component of following parts by weight, matrimony vine stem cell 2
~8 parts, 5~12 parts of Herba Dendrobii extract, 10~15 parts of bletilla striata extract, 6~12 parts of coptis extract, chelating agent 0.01-0.1
Part, 5-18 parts of moisturizer, 0.3-0.6 parts of preservative, 80~150 parts of deionized water.
Moisturizer is Sodium Hyaluronate, a kind of in glycerine, propane diols, Fructus Hordei Germinatus oligose base glucosides；
Preservative is methyl hydroxybenzoate, Phenoxyethanol/at least one of Sensiva SC50 or Kathon CG.
Chelating agent is a kind of in EDTA- disodiums or Etidronic Acid.
The preparation method of above-mentioned skin care item, comprises the following steps：
1) preparation of Chinese wolfberry fruit dry cell extract：A) matrimony vine of the selection without fertilizer and pesticide pollution, the organic plantation of green, and press
The newborn branch of ten 10cm long of criterion picks, with aseptic water washing matrimony vine branch surface attachments 15~20 minutes, by new life
Branch, which is put into, soaks 1min in the ascorbic acid of 110mg/L, then carries out surface sterilization 30 seconds with 75% alcohol, then, uses
0.1% mercuric chloride liquid disinfectant 10~20 minutes, is finally rinsed 5 times repeatedly with sterile water, washes away the mercuric chloride raffinate of plant surface；b)
Matrimony vine branch after sterilizing vaccinating lancet is peeled off into forming layer, bast, cortex and epidermal tissue, abandons xylem and marrow, will
The tissue of acquisition is seeded in fresh inducing culture, and inoculation 10cm is organized with every 1g2Inducing culture density carry out
Inoculation, continuously cultivates 10 days for 25 DEG C in controlled darkroom, obtains Cambium tissue；C) by regenerated Cambium tissue and other
Tissue separates, and inoculation solid medium 10cm is organized with every 1g2Inducing culture density, by it is regenerated formation tissue inoculation
Into fresh inducing culture, continuously cultivated 10 days for 25 DEG C in controlled darkroom, obtain callus；D) 1000ml's
200ml proliferated culture mediums, the 20g of sterilization treatment, the bead of diameter 0.5cm are added in triangular flask, 10g callus is moved to
In triangular flask, triangular flask is placed in 25 DEG C, is cultivated on the shaking table of 120r/min, is shone per hour with the incandescent lamp of 20w
10min is penetrated, the 7th day, adds 1 × 10 of fresh 400ml fluid nutrient mediums to cell density for wherein cell density5/ml,
Culture 7 days is then proceeded to, is filtered after culture with the stainless steel mesh of 100 mesh, is removed cell mass, residue etc., obtain matrimony vine cell
Suspension；E) matrimony vine cell suspending liquid is moved to and large-scale culture is carried out in 20L bioreactors, bioreactor accommodates the increasing of 5L
Culture medium is grown, cultivates the 7th day proliferated culture medium for adding 10L, cell suspending liquid is obtained after continuing culture.F) collection step e)
Middle acquisition matrimony vine stem cell suspension, is filtered with 1um filters, removes culture medium, harvest the cell of retention；And be put into cell-
When 80 DEG C of superfreeze 2 is small, defrosting 30 minutes is then taken out, repeats aforesaid operations 5 times, after last time thaw at RT,
With sonicator smudge cells, after filter filtering, filtrate is collected, in being concentrated in rotating vacuum evaporator, you can obtain
Obtain Chinese wolfberry fruit dry cell extract.
Inducing culture：NAA0.5mg/L+BA 2.0mg/L and caseinhydrolysate 1mg/L+MS solid mediums, pH value are
6.0；Proliferated culture medium：2,4-D 2.5mg/L+BA1.5mg/L+ caseinhydrolysates 1mg/L and activated carbon 1mg/L+MS liquid are trained
Support base；PH value is 6.0.
Matrimony vine stem cell, Herba Dendrobii extract, bletilla striata extract, coptis extract are realized using the prior art.
2) by Chinese wolfberry fruit dry cell extract, Herba Dendrobii extract, bletilla striata extract, coptis extract, moisturizer, preservative,
Carbomer, chelating agent, deionized water are added in pot, are heated to 85~90 DEG C, stirring and dissolving, the cooling of open cycle water, keeps
Stir and vacuumize defoaming；When continuing to be cooled to 36 DEG C, discharging, gets product.
Embodiment 1
It is a kind of to dispel blackhead skin care item containing Chinese wolfberry fruit dry cell extract, include the component of following parts by weight, matrimony vine stem cell 2
Part, 5 parts of Herba Dendrobii extract, 10 parts of bletilla striata extract, 6 parts of coptis extract, EDTA- disodiums are 0.01 part, Sodium Hyaluronate 5
Part, 0.3 part of methyl hydroxybenzoate, 80 parts of deionized water.
The present embodiment preparation method is as described above.
Embodiment 2
It is a kind of to dispel blackhead skin care item containing Chinese wolfberry fruit dry cell extract, include the component of following parts by weight, matrimony vine stem cell 3
Part, 6 parts of Herba Dendrobii extract, 10 parts of bletilla striata extract, 8 parts of coptis extract, 0.02 part of Etidronic Acid, 8 parts of glycerine, oxybenzene first
0.35 part of ester, 85 parts of deionized water.The present embodiment preparation method such as embodiment 1.
Embodiment 3
It is a kind of to dispel blackhead skin care item containing Chinese wolfberry fruit dry cell extract, include the component of following parts by weight, matrimony vine stem cell 4
Part, 8 parts of Herba Dendrobii extract, 12 parts of bletilla striata extract, 7.5 parts of coptis extract, 0.03 part of Etidronic Acid, 10 parts of propane diols,
0.5 part of Phenoxyethanol, 90 parts of deionized water.The present embodiment preparation method such as embodiment 1.
Embodiment 4
It is a kind of to dispel blackhead skin care item containing Chinese wolfberry fruit dry cell extract, include the component of following parts by weight, matrimony vine stem cell 5
Part, 7.5 parts of Herba Dendrobii extract, 13 parts of bletilla striata extract, 8.5 parts of coptis extract, EDTA- disodiums are 0.05 part, Fructus Hordei Germinatus oligose
10 parts of base glucosides, 0.4 part of Sensiva SC50,100 parts of deionized water.The present embodiment preparation method such as embodiment 1.
Embodiment 5
It is a kind of to dispel blackhead skin care item containing Chinese wolfberry fruit dry cell extract, include the component of following parts by weight, matrimony vine stem cell 6
Part, 10 parts of Herba Dendrobii extract, 12.5 parts of bletilla striata extract, 10 parts of coptis extract, EDTA- disodiums are 0.08 part, hyaluronic acid
12 parts of sodium, 0.4 part of methyl hydroxybenzoate, 110 parts of deionized water.The present embodiment preparation method such as embodiment 1.
Embodiment 6
It is a kind of to dispel blackhead skin care item containing Chinese wolfberry fruit dry cell extract, include the component of following parts by weight, matrimony vine stem cell
6.5 parts, 9.5 parts of Herba Dendrobii extract, 14 parts of bletilla striata extract, 10.5 parts of coptis extract, 0.08 part of Etidronic Acid, glycerine 15
Part, 0.5 part of Phenoxyethanol, 120 parts of deionized water.The present embodiment preparation method such as embodiment 1.
Embodiment 7
It is a kind of to dispel blackhead skin care item containing Chinese wolfberry fruit dry cell extract, include the component of following parts by weight, matrimony vine stem cell
7.5 parts, 11 parts of Herba Dendrobii extract, 14.5 parts of bletilla striata extract, 11 parts of coptis extract, chelating agent is 0.1 part, propane diols 16
Part, 0.5 part of Kathon CG, 130 parts of deionized water.The present embodiment preparation method such as embodiment 1.
Embodiment 8
It is a kind of to dispel blackhead skin care item containing Chinese wolfberry fruit dry cell extract, include the component of following parts by weight, matrimony vine stem cell 8
Part, 12 parts of Herba Dendrobii extract, 15 parts of bletilla striata extract, 12 parts of coptis extract, chelating agent are 0.08 part, Fructus Hordei Germinatus oligose base glucosides
18 parts, 0.6 part of Sensiva SC50,150 parts of deionized water.The present embodiment preparation method such as embodiment 1.
Control group
Table 1 is the traditional Chinese medicine ingredients and content of control group skin care item
Control group 17 is dispelled blackhead skin care item using hundred sparrow antelopes of commercial type.
The content for traditional Chinese medicine ingredients for the skin care item listed in table 1, content and the component and 4 phase of embodiment of remaining auxiliary agent
Together.
Experiment effect
1st, guinea pig skin stimulation test
1~8 composite skin care product of 0.5g embodiments is respectively taken, being diluted to 100g using deionized water is configured to given the test agent.
Given the test agent using skin irritation test method (《Cosmetics health specification》2007 editions toxicology test methods) tested.
Experimental animal：Cavy weight 250g~300g.
Experimental result：Through 14 days Skin Irritation Tests, stimulate the reaction is had no.
2nd, Efficacy experiments of the present invention
2.1 number of cases on probation：Select age bracket to have the volunteer of blackhead in the nose of 25 years old to 45 years old, be divided into 25 groups, every group 50
Example, male 25, women 25；
2.2 application method：Test group prepares skin care item using embodiment 1~8, thoroughly cleans face skin, morning and evening each one
Secondary use and nose, after 10-15 minutes, are gently wiped away, 1 time every three days with the fur towel of moistening, use were checked using 30 days
Effect.
2.3 use result
As can be seen from Table 2, production of the present invention is shown more than 88% using the blackhead skin care item of dispelling of the present invention, its effective percentage
Product have good therapeutic effect to blackhead of dispelling, and wherein embodiment 2 to 8 is more than 90%.
Control group 1~4 is respectively only using Chinese wolfberry fruit dry cell extract, Herba Dendrobii extract, bletilla striata extract, coptis extraction
One kind in thing, its effective percentage have certain blackhead effect of dispelling between 54%~60%, but ineffective, and control group 5~
10 be that skin care item are made using both any combinations in four kinds of extracts, its effective percentage is between 62~68%, it can be seen that phase
For a kind of extract, its effect has lifting, and control group 11~14 is that its is effective using any three kinds of combinations in four kinds of extracts
Between 68~76%, it is combined rate with both, is not much different, and the present invention selects four kinds and combines at the same time, its effect obtains very
Big lifting, illustrates only under four kinds of extracts jointly synergistic effect, competence exertion optimum efficiency.
Control group 15~16 is under the conditions of the identical composition of use, identical preparation method, its formula rate exists
Outside the specific proportion of the present invention, its effect is nothing like the effect of the present invention, it was demonstrated that, composition of the invention only has
Competence exertion is significantly dispelled blackhead effect in special ratios.Control group 17 is to use commercial product, it can be seen that effect is not as this
Invention, illustrates the present invention relative to the prior art has significant anti-acne effect.
The foregoing is merely illustrative of the preferred embodiments of the present invention, is not intended to limit the invention, all essences in the present invention
God and any modification, equivalent substitution, improvement and etc. within principle, done, should be included within the scope of protection of the invention.
Claims (10)
- The blackhead skin care item 1. one kind is dispelled, it is characterised in that include the component of following parts by weight, Chinese wolfberry fruit dry cell extract 2~8 Part, 5~12 parts of Herba Dendrobii extract, 10~15 parts of bletilla striata extract, 6~12 parts of coptis extract.
- 2. blackhead skin care item according to claim 1 of dispelling, it is characterised in that include the component of following parts by weight, Chinese wolfberry fruit dry 3~7.5 parts of cell extract, 6~11 parts of Herba Dendrobii extract, 10~14 parts of bletilla striata extract, 7.5~11 parts of coptis extract.
- 3. blackhead skin care item according to claim 1 of dispelling, it is characterised in that include the component of following parts by weight, Chinese wolfberry fruit dry 4~6.5 parts of cell extract, 8~10 parts of Herba Dendrobii extract, 12~14 parts of bletilla striata extract, coptis extract 7.5~10.5 Part.
- 4. blackhead skin care item according to claim 1 of dispelling, it is characterised in that include the component of following parts by weight, Chinese wolfberry fruit dry 5~6.5 parts of cell extract, 7.5~10 parts of Herba Dendrobii extract, 12.5~14 parts of bletilla striata extract, coptis extract 8.5~ 10.5 parts.
- 5. blackhead skin care item according to claim 1 of dispelling, it is characterised in that include the component of following parts by weight, Chinese wolfberry fruit dry 5~6 parts of cell extract, 7.5~9.5 parts of Herba Dendrobii extract, 12.5~13 parts of bletilla striata extract, coptis extract 8.5~10 Part.
- 6. blackhead skin care item according to claim 1 of dispelling, it is characterised in that include the component of following parts by weight, Chinese wolfberry fruit dry 5 parts of cell, 7.5 parts of Herba Dendrobii extract, 13 parts of bletilla striata extract, 8.5 parts of coptis extract.
- 7. blackhead skin care item according to claim 1 of dispelling, it is characterised in that include the component of following parts by weight, Chinese wolfberry fruit dry 6 parts of cell, 10 parts of Herba Dendrobii extract, 12.5 parts of bletilla striata extract.
- 8. blackhead skin care item according to claim 1 of dispelling, it is characterised in that include the component of following parts by weight, Chinese wolfberry fruit dry 6.5 parts of cell, 9.5 parts of Herba Dendrobii extract, 14 parts of bletilla striata extract, 10.5 parts of coptis extract.
- 9. blackhead skin care item according to claim 1 of dispelling, it is characterised in that further include the component of following parts by weight, chelate Agent is 0.01-0.1 parts, 5-18 parts of moisturizer, 0.3-0.6 parts of preservative, 80~150 parts of deionized water；The moisturizer is Sodium Hyaluronate, a kind of in glycerine, propane diols, Fructus Hordei Germinatus oligose base glucosides；The preservative is methyl hydroxybenzoate, Phenoxyethanol/at least one of Sensiva SC50 or Kathon CG；The chelating agent is a kind of in EDTA- disodiums or Etidronic Acid.
- The blackhead skin care item preparation method 10. a kind of such as claim 1 to 9 any one of them is dispelled, it is characterised in that including following Step：1) preparation of the Chinese wolfberry fruit dry cell extract：A) after the new branch sterilization of matrimony vine, xylem and marrow being removed, it is inoculated in and lures Culture medium is led, inducing culture obtains cambial cell；B) cambial cell through culture medium through subculture, access proliferated culture medium, Shaking table culture obtains unicellular；C) the unicellular proliferated culture medium that is inoculated in is cultivated through expanding, and obtains matrimony vine stem cell；D) institute State the matrimony vine stem cell after amplification it is chilled it is broken after concentrate, that is, harvest Chinese wolfberry fruit dry cell extract；2) by the Chinese wolfberry fruit dry cell extract, Herba Dendrobii extract, bletilla striata extract, coptis extract, moisturizer, preservative, Carbomer, chelating agent, deionized water are added in pot, are heated to 85~90 DEG C, stirring and dissolving, the cooling of open cycle water, keeps Stir and vacuumize defoaming；When continuing to be cooled to 36 DEG C, discharging, gets product.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201711271900.XA CN108030755A (en) | 2017-12-05 | 2017-12-05 | It is a kind of to dispel blackhead skin care item and preparation method thereof containing Chinese wolfberry fruit dry cell extract |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201711271900.XA CN108030755A (en) | 2017-12-05 | 2017-12-05 | It is a kind of to dispel blackhead skin care item and preparation method thereof containing Chinese wolfberry fruit dry cell extract |
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| CN108030755A true CN108030755A (en) | 2018-05-15 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201711271900.XA Withdrawn CN108030755A (en) | 2017-12-05 | 2017-12-05 | It is a kind of to dispel blackhead skin care item and preparation method thereof containing Chinese wolfberry fruit dry cell extract |
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| CN (1) | CN108030755A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108785207A (en) * | 2018-08-10 | 2018-11-13 | 广州碧雅医疗保健科技有限公司 | A kind of anti-aging of the ingredient containing Chinese medicine compound prescription is preserved youthful looks skin care compositions and skin care item |
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| CN103976920A (en) * | 2014-05-31 | 2014-08-13 | 广州丹奇日用化工厂有限公司 | Shinyleaf pricklyash root extracting solution as well as preparation method and application thereof |
| CN104711215A (en) * | 2015-04-09 | 2015-06-17 | 广州赛莱拉干细胞科技股份有限公司 | Apple stem cell culture method and apple stem cells cultured by method |
| CN105748343A (en) * | 2016-02-25 | 2016-07-13 | 广州市科玮生物技术有限公司 | Natural plant extract composition and application thereof in cosmetics |
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2017
- 2017-12-05 CN CN201711271900.XA patent/CN108030755A/en not_active Withdrawn
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| CN103976920A (en) * | 2014-05-31 | 2014-08-13 | 广州丹奇日用化工厂有限公司 | Shinyleaf pricklyash root extracting solution as well as preparation method and application thereof |
| CN104711215A (en) * | 2015-04-09 | 2015-06-17 | 广州赛莱拉干细胞科技股份有限公司 | Apple stem cell culture method and apple stem cells cultured by method |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108785207A (en) * | 2018-08-10 | 2018-11-13 | 广州碧雅医疗保健科技有限公司 | A kind of anti-aging of the ingredient containing Chinese medicine compound prescription is preserved youthful looks skin care compositions and skin care item |
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