CN107996395A - A kind of selection of low critical temperature of sterility two-line sterile line rice - Google Patents
A kind of selection of low critical temperature of sterility two-line sterile line rice Download PDFInfo
- Publication number
- CN107996395A CN107996395A CN201810093483.2A CN201810093483A CN107996395A CN 107996395 A CN107996395 A CN 107996395A CN 201810093483 A CN201810093483 A CN 201810093483A CN 107996395 A CN107996395 A CN 107996395A
- Authority
- CN
- China
- Prior art keywords
- line
- sterile line
- sterility
- critical temperature
- rice
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
- A01H1/02—Methods or apparatus for hybridisation; Artificial pollination ; Fertility
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
- A01H1/04—Processes of selection involving genotypic or phenotypic markers; Methods of using phenotypic markers for selection
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- General Health & Medical Sciences (AREA)
- Botany (AREA)
- Developmental Biology & Embryology (AREA)
- Environmental Sciences (AREA)
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
Abstract
The present invention provides a kind of selection of low critical temperature of sterility two-line sterile line rice, comprise the following steps：Cultivate the excellent two-line sterile line rice of Comprehensive Traits；Screen low critical temperature of sterility sterile line rice and low critical temperature of sterility sterile line rice under screening short-day under the long-day；And the low critical temperature of sterility two-line sterile line rice of Integrated Selection；Removal of impurities purification is carried out with to two-line sterile line rice.The present invention utilizes local long-day low temperature screening and the screening of Hainan short-day low temperature, by setting interval sowing, using the two-line sterile line of low critical temperature of sterility as control, low critical temperature of sterility selection and breeding problem can effectively solve the problem that to fertility microscopy and bagging inspection, the good low critical temperature of sterility two-line sterile line of eurytopicity can be filtered out.
Description
Technical field
The invention belongs to crop breeding field, and in particular to a kind of selection and breeding of low critical temperature of sterility two-line sterile line rice
Method.
Background technology
Double-hybrid rice strains is the new and high technology for being utilized as main contents with Photo-and thermo-sensitive genic sterility of China's original creation,
It is after the important technical innovation again after Three-line Hybrid rice in Crop Genetic Breeding.Under in the eighties in last century the Changjiang river
The regional midsummer abnormal low temperature of trip causes the double-linear hybrid rice production of hybrid seeds failure of domestic large area at that time, causes two-line sterile line of rice
Also two-line sterile line of rice new frame based on temperature-sensitive sterile line is developed into by rice photosensitive-sensitive sterile line originally, proposed
The fertility standard of practical two-line sterile line, which is that selection and breeding critical temperature of sterility is relatively low, (is less than 23.5 DEG C, so that the production of hybrid seeds is pacified
Entirely), the sterile line of light temperature complementation relatively strong (in favor of sterile line propagation).
The cultivation of low critical temperature of sterility sterile line is the key point and difficult point of double-linear hybrid rice breeding.Safety in production
Below 23.5 DEG C, upper production is upper at present mainly utilizes intelligence for the critical temperature of sterility requirement of the photo-thermo sensitive male nuclear sterile rice line of application
Can the low critical temperature of sterility sterile line of temperature control constant temperature water cooling pond screening.Intelligent temperature control constant temperature water cooling pond has high equipment cost, answers
With it is of high cost, time-consuming, treating capacity is small, cannot solve fertility drift about the problems such as, for the above problem existing for conventional art,
Propose the new method that low critical temperature of sterility two-line sterile line is screened using natural, ecological low temperature.
The content of the invention
In view of this, it is an object of the invention to provide a kind of natural low temperature using under the conditions of long-day, short-day, and
The selection for setting low critical temperature of sterility sterile line to compare, has the characteristics that cost is low, effect is good, simple and easy to do, energy
The problems such as overcoming small high cost, the treating capacity of conventional art, fertility drift, and the sterile line practicality of selection and breeding is good.
I.e. the first object of the present invention is to provide a kind of selection of low critical temperature of sterility two-line sterile line rice,
Comprise the following steps：
1) the excellent two-line sterile line rice of Comprehensive Traits, is cultivated；
2) low critical temperature of sterility sterile line rice under the long-day, is screened；
3) low critical temperature of sterility sterile line rice under short-day, is screened；
4), the low critical temperature of sterility two-line sterile line rice of Integrated Selection；
5) removal of impurities purification, is carried out to two-line sterile line rice.
Selection and breeding base in the present invention --- Chongqing and Hainan base are all located at the Northern Hemisphere, and the positive season two-line sterile line in Chongqing is educated
Property sensitive periods, the duration of day was more than 13.5h, is defined as the long-day in mid or late June.During fertility-sensitive period is on November when autumn is numerous
Ten days, the duration of day are less than 12h, are short-day.High temperature in the present invention in selection and breeding temperature refer to ambient temperature apparently higher than
Sterile line critical temperature of sterility, on the contrary low temperature refers to that environment temperature is less than sterile line critical temperature of sterility.
Preferably, in the selection of low critical temperature of sterility two-line sterile line rice of the present invention, the step
1) the excellent two-line sterile line rice of Comprehensive Traits is the ecology screening of Chongqing rice-regeneration season in, and the Comprehensive Traits are excellent
It is good including sterility degree under long day high temperature up to 100%, strain leaf character is excellent, rice quality up to gold standard, rice blast resistance not
Stablize relatively higher than 5 grades, heredity.
The identification of above merit can refer to two-line sterile line parent authorization or standard of perfection.
Preferably, in the selection of low critical temperature of sterility two-line sterile line rice of the present invention, the step
1) breeding method described in includes Anther Culture fast and stable technology.
Preferably, in the selection of low critical temperature of sterility two-line sterile line rice of the present invention, the step
2) screening technique is to utilize influence of the low temperature to two-line sterile line booting sensitive periods under Hainan Island autumn numerous short-day, interval sowing
Screen low critical temperature of sterility two-line sterile line rice.
Preferably, in the selection of low critical temperature of sterility two-line sterile line rice of the present invention, the step
2) screening technique further comprises the steps：
1. setting interval sowing, interval sowing under the positive long-day in season of Chongqing, each issue of single sterile line material presses 200 nest list seedlings
Transplanting；
2. select check variety：The same period sets low critical temperature of sterility two-line sterile line as control；
3. recording booting sensitive periods temperature, the sterile line fertility-sensitive period date is determined；
4. Fertility evaluation and screening：Environment mean daily temperature is less than 23 degree of date of seeding sterile line above for three days on end,
Full heading time bagging is selfed, each 5-10 bags of sterile line material bagging, and takes clever flower pesticide mixing film-making, micro- Microscopic observation sterile line
Fertility, to compare sterile line as reference, selects microscopy, bagging robustness is better than or the new sterile line suitable with control.Preferably,
In the selection of low critical temperature of sterility two-line sterile line rice of the present invention, the interval sowing of the step 1. is 4
Phase is sowed；It is highly preferred that the sowing time first phase is September 1, the second phase is September 8, and the third phase is September 15, the fourth phase 9
The moon 25；Step 2. in low critical temperature of sterility two-line sterile line be C815S, Y58S and depth 08S is control.
Preferably, in the selection of low critical temperature of sterility two-line sterile line rice of the present invention, the step
3) screening technique further comprises the steps：
1. interval sowing is set, the interval sowing under autumn numerous short-day；
2. select check variety：The same period sets low critical temperature of sterility two-line sterile line as control；
3. recording booting sensitive periods temperature, the sterile line fertility-sensitive period date is determined；
4. Fertility evaluation and screening：Environment mean daily temperature is less than 23 degree of date of seeding sterile line above for three days on end,
Full heading time bagging is selfed, each 5-10 bags of sterile line material bagging, and takes clever flower pesticide mixing film-making, micro- Microscopic observation sterile line
Fertility, to compare sterile line as reference, selects microscopy, bagging robustness is better than or the new sterile line suitable with control.
Preferably, in the selection of low critical temperature of sterility two-line sterile line rice of the present invention, the step
1. interval sowing include the sowing time first phase for September 1 day, the second phase is September 8 days, and the third phase is September 15 days, the fourth phase 9
The moon 25；, the step 2. in low critical temperature of sterility two-line sterile line be C815S, Y58S and depth 08S is control.
Preferably, in the selection of low critical temperature of sterility two-line sterile line rice of the present invention, the step
4) be according under the long-day, under short-day under fertility-sensitive period low temperature the Fertility evaluation of two-line sterile line and screening situation, screening
Go out under long-day, short-day sterile degree all better than control or suitable with control sterile line two be new sterile line, as it is low not
Educate origin temp two-line sterile line rice.
Another object of the present invention is the low critical temperature of sterility two-line sterile line that the above method obtains.
Compared with prior art, the present invention the present invention has the following advantages：The screening of low critical temperature of sterility sterile line is
The key point and difficult point of double-hybrid rice strains breeding, are screened using the screening of local long-day low temperature and Hainan short-day low temperature,
, can be effective to fertility microscopy and bagging inspection using the two-line sterile line of critical temperature of sterility as control by setting interval sowing
Solve the problems, such as low critical temperature of sterility selection and breeding, filter out the good low critical temperature of sterility two-line sterile line of eurytopicity.
Brief description of the drawings
Fig. 1 is the selection schematic diagram of the low critical temperature of sterility two-line sterile line rice of the present invention.
Embodiment
Below in conjunction with the embodiment in the present invention, the technical solution in the present invention is clearly and completely described.It is aobvious
So, described embodiment is only part of the embodiment of the present invention, instead of all the embodiments.Based on the reality in the present invention
Apply example, those of ordinary skill in the art's all other embodiments obtained without making creative work, all belong to
In the scope of protection of the invention.
The selection and breeding of the low critical temperature of sterility two-line sterile line rice 924S of embodiment 1
The excellent two-line sterile line of 1.1 Comprehensive Traits is cultivated
As shown in Figure 1, using the ecological breeding condition of Chongqing rice-regeneration, with reference to Anther Culture fast and stable technology,
Cultivate that sterile degree 100%, strain leaf character under long day high temperature be excellent, rice quality is not higher than five up to gold standard, rice blast resistance
Level, heredity metastable two are new sterile line 924S, 935S, 938S.
The screening of low critical temperature of sterility sterile line under 1.2 long-day
It is the influence of rice sterile line booting sensitive periods to two using low temperature under the positive long-day in season of Chongqing, interval sowing screens
Low critical temperature of sterility two-line sterile line.
1. interval sowing is set：According to Chongqing climatic characteristic, the sowing of 4 phases of sterile line point, sowing time 3/6 first phase, second
Sowing time 3/16 phase, the third phase 3/26, the sowing time fourth phase are 4/5, and each issue of single sterile line material is unified by 200 nests, Dan Miao
Transplanting.
2. select check variety：It is pair that the same period, which sets low critical temperature of sterility two-line sterile line C815S, Y58S and depth 08S,
According to the Fertility Transfer Temperature of 3 control sterile lines of the above is not higher than 23 degrees Celsius.
3. booting sensitive periods temperature is recorded：Equipped with high-precision weather station near experimental plot, daily mean temperature is recorded.
Into after boot stage, the sterile line fertility-sensitive period date is determined by shelling neck, is recorded in detail.
4. Fertility evaluation and screening：According to weather station fertility-sensitive period temperature statistics, environment mean daily temperature for three days on end with
The upper sowing time less than 23 degree is the sterile line by stages of sowing on March 6, and each sterile line and control sterile line beginning ear period are recorded in observation
And full heading time, it is selfed in full heading time bagging, each 5-10 bags of sterile line material bagging, the same period takes each sterile line grain husk flower flower pesticide mixing
Film-making, micro- Microscopic observation infertility line fertility.To compare sterile line as reference, 924S is in sterile degrees such as microscopy, bagging robustnesses
It is suitable with control
1.3 the screening of low critical temperature of sterility sterile line under short-day
Using influence of the low temperature to two-line sterile line booting sensitive periods under Hainan Island autumn numerous short-day, interval sowing screening is low
Critical temperature of sterility two-line sterile line.
1. interval sowing：According to rice autumn numerous low temperature occurrence law, also divided for 4 phases sowed, the sowing time first phase is 9/1, the
Second phase is 9/8, the third phase 9/15, the fourth phase 9/25,
2. control is set：It is control that each issue, which sets low critical temperature of sterility two-line sterile line C815S, Y58S and depth 08S,.
3. booting sensitive periods temperature is recorded：Field is furnished with Portable weather station, records daily mean temperature and sunshine length
Degree.Into after boot stage, sterile line booting date sensitive periods is determined by shelling neck, is recorded in detail.
4. Fertility evaluation and screening：According to weather station fertility-sensitive period temperature statistics, environment mean daily temperature for three days on end with
Above the sowing time less than 23 degree is September 1, remembers to be measured and control sterile line beginning ear period and full heading time, in full heading time bagging certainly
Hand over, each 5 bags -10 bags of sterile line material bagging, and take grain husk flower flower pesticide mixing film-making, micro- Microscopic observation infertility line fertility and set
The solid situation of bag compares, and 924S sterile degrees are slightly better than control depth 08S etc..
The Integrated Selection of 1.4 low critical temperature of sterility two-line sterile lines
According under the long-day, under short-day under fertility-sensitive period low temperature the Fertility evaluation of two-line sterile line and screening situation,
Comprehensive sterile line each under long-day, short-day is low two system of critical temperature of sterility with compareing sterile line comparable situation, 924S
Sterile line.
The removal of impurities purification of 1.5 two-line sterile lines
924S is screened in the sterile line of September sowing on the 1st, sterile degree is not thorough or the single plant of fertility drift carries out
Removing is handled, the 924S irrigation fertilization management after removal of impurities, and stake processing is cut in early January, and is isolated before heading or bagging
Ensure purity, it the two of low critical temperature of sterility is new sterile line that the seed after removal of impurities purification, which is,.
Identified by later stage variety and quality：924S has the characteristics such as strain leaf character is excellent, critical temperature of sterility is low,
Autumn in 2017 is numerous to test by stages, and the first phase meets low temperature, and under the background that other sterile lines recover fertility, 924S is shown preferably
Low critical temperature of sterility characteristic.
The above is only the preferred embodiment of the present invention, it is noted that for the ordinary skill people of the art
For member, various improvements and modifications may be made without departing from the principle of the present invention, these improvements and modifications also should
It is considered as protection scope of the present invention.
Claims (10)
1. a kind of selection of low critical temperature of sterility two-line sterile line rice, comprises the following steps：
1) the excellent two-line sterile line rice of Comprehensive Traits, is cultivated；
2) low critical temperature of sterility sterile line rice under the long-day, is screened；
3) low critical temperature of sterility sterile line rice under short-day, is screened；
4), the low critical temperature of sterility two-line sterile line rice of Integrated Selection；
5) removal of impurities purification, is carried out to two-line sterile line rice.
2. the selection of low critical temperature of sterility two-line sterile line rice according to claim 1, it is characterised in that institute
It is the season ecology selection and breeding of Chongqing rice-regeneration to state the excellent two-line sterile line rice of Comprehensive Traits in step 1), described comprehensive
Shape is excellent including sterility under long day high temperature is good, strain leaf character is excellent, rice quality is relatively excellent, rice blast resistance is good, hereditary phase
To stabilization.
3. the selection of low critical temperature of sterility two-line sterile line rice according to claim 1, it is characterised in that institute
Stating breeding method described in step 1) includes Anther Culture fast and stable technology.
4. the selection of low critical temperature of sterility two-line sterile line rice according to claim 1, it is characterised in that institute
The screening technique for stating step 2) is using influence of the low temperature to two-line sterile line booting sensitive periods under Hainan Island autumn numerous short-day, is divided
Low critical temperature of sterility two-line sterile line rice is screened in phase sowing.
5. the selection of low critical temperature of sterility two-line sterile line rice according to claim 4, it is characterised in that institute
The screening technique for stating step 2) further comprises the steps：
1. interval sowing is set, interval sowing under the positive long-day in season of Chongqing, each issue of single 200 nest list transplantation of seedlings of sterile line material；
2. select check variety：The same period sets the Fertility Transfer Temperature to be not higher than 23 DEG C of low critical temperature of sterility two-line sterile line
Control；
3. recording booting sensitive periods temperature, the sterile line fertility-sensitive period date is determined；
4. Fertility evaluation and screening：Recorded according to temperature, Environment mean daily temperature for three days on end broadcast above by dividing less than 23 degree
Sterile line, is selfed, each 5-10 bags of sterile line material bagging in full heading time bagging, and takes clever flower pesticide mixing film-making, under microscope
The sterile line fertility of observation, to compare sterile line as reference, selects microscopy, bagging robustness is better than or the new infertility suitable with control
System.
6. the selection of low critical temperature of sterility two-line sterile line rice according to claim 4, it is characterised in that institute
The interval sowing of step 1. is stated to sow for 4 phases；It is highly preferred that the sowing time first phase is September 1, the second phase is September 8, the 3rd
Phase is September 15, and the fourth phase is September 25；Step 2. in low critical temperature of sterility two-line sterile line be C815S, Y58S and depth
08S is control, and the critical temperature of sterility of 3 sterile lines of the above is all not higher than 23 DEG C.
7. the selection of low critical temperature of sterility two-line sterile line rice according to claim 1, it is characterised in that institute
The screening technique for stating step 3) further comprises the steps：
1. interval sowing is set, the interval sowing under autumn numerous short-day；
2. select check variety：The same period sets low critical temperature of sterility two-line sterile line as control；
3. recording booting sensitive periods temperature, the sterile line fertility-sensitive period date is determined；
4. Fertility evaluation and screening：Environment mean daily temperature is less than 23 degree of sowing time sterile line above for three days on end, neat
Ear period bagging is selfed, each 5-10 bags of sterile line material bagging, and takes clever flower pesticide mixing film-making, and micro- Microscopic observation sterile line is educated
Property, to compare sterile line as reference, select microscopy, bagging robustness is better than or the new sterile line suitable with control.
8. the selection of low critical temperature of sterility two-line sterile line rice according to claim 8, it is characterised in that institute
To state the interval sowing of step 1. and be September 1 including the sowing time first phase, the second phase is September 8, and the third phase is September 15, the
The fourth phase is September 25 days, the step 2. in low critical temperature of sterility two-line sterile line be C815S, Y58S and depth 08S is control.
9. the selection of low critical temperature of sterility two-line sterile line rice according to claim 1, it is characterised in that institute
State step 4) be according under the long-day, under short-day under fertility-sensitive period low temperature the Fertility evaluation of two-line sterile line and screening feelings
Condition, filters out under long-day, short-day sterile degree all better than control or suitable with control sterile line two is new sterile line, i.e.,
For low critical temperature of sterility two-line sterile line rice.
10. the low critical temperature of sterility two-line sterile line obtained according to claim 1~9 any one method.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810093483.2A CN107996395A (en) | 2018-01-31 | 2018-01-31 | A kind of selection of low critical temperature of sterility two-line sterile line rice |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810093483.2A CN107996395A (en) | 2018-01-31 | 2018-01-31 | A kind of selection of low critical temperature of sterility two-line sterile line rice |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN107996395A true CN107996395A (en) | 2018-05-08 |
Family
ID=62066465
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201810093483.2A Pending CN107996395A (en) | 2018-01-31 | 2018-01-31 | A kind of selection of low critical temperature of sterility two-line sterile line rice |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN107996395A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109618920A (en) * | 2019-02-23 | 2019-04-16 | 重庆市农业科学院 | A kind of hybrid paddy rice dual purpose genic male sterile line clonal regeneration pure keeping method |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105359964A (en) * | 2015-12-10 | 2016-03-02 | 重庆市农业科学院 | Efficient breeding method of two-line hybrid rice sterile lines |
-
2018
- 2018-01-31 CN CN201810093483.2A patent/CN107996395A/en active Pending
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105359964A (en) * | 2015-12-10 | 2016-03-02 | 重庆市农业科学院 | Efficient breeding method of two-line hybrid rice sterile lines |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109618920A (en) * | 2019-02-23 | 2019-04-16 | 重庆市农业科学院 | A kind of hybrid paddy rice dual purpose genic male sterile line clonal regeneration pure keeping method |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| US11246275B2 (en) | Method for breeding small-grain sterile rice line and simple method for producing hybrid rice seed | |
| CN105850622B (en) | A kind of high temperature leads to the identification method of rice grain infertility | |
| CN104542247B (en) | A kind of selection of paddy rice dual-purpose caryon sterile line | |
| CN104737898A (en) | Fast creating method for rice long-grain japonica type indica-compatible two-line sterile line germplasm | |
| CN110122316A (en) | The photosensitive kernel male sterile mutant of cotton and its application | |
| CN105359964B (en) | One kind two is the efficient selection of rice sterile line | |
| Li et al. | Fertility alteration in the photo-thermo-sensitive male sterile line BS20 of wheat (Triticum aestivum L.) | |
| CN107996395A (en) | A kind of selection of low critical temperature of sterility two-line sterile line rice | |
| CN102100175A (en) | Breeding and hybrid seed-producing method of onion male sterile line | |
| CN100435624C (en) | Breeding method for chilli pepper nuclear male sterile dual purpose line and nuclear substance male sterile recovery line | |
| CN102550394B (en) | Cold-region japonica hybrid rice restorer and variety breeding method | |
| BAGHERI et al. | Evaluation of quantity and quality characteristics of sugar beet varieties in different sowing date of direct sowing and transplanting in Shirvan and Mashhad | |
| CN106332770A (en) | Cold region hybrid japonica rice breeding method | |
| CN109349102A (en) | A kind of long grain japonica rice two-line sterile line selection | |
| CN101663994A (en) | Seed production method of hybridized summer-sown soybean | |
| CN100464630C (en) | Light temperature sensitive two-line cross breeding wheat cover film hybrid seed producing method | |
| CN107593435B (en) | The identification of strong cold-resistant Chinese cabbage type winter rape study on temperature sensitive male sterility mutant and screening technique | |
| CN101779597A (en) | Breeding method for inducing wheat physiological male sterility | |
| CN111165343A (en) | Breeding method of dual-purpose genic male sterile line with high outcrossing rate and high combining ability | |
| CN1672503B (en) | Light and temperature interacting propagation method for cotton nuclear male sterile line Yahuang A | |
| CN104170718A (en) | Method for cultivating photosensitive genic sterile line with long fertility transition critical illumination length | |
| CN103098702B (en) | Breeding method of K type thermosensitive male sterile wheat | |
| Rajesh et al. | Critical stages of thermo-sensitive genic male sterile lines of rice in Kerala | |
| CN113973708A (en) | Method for judging whether forage grass sorghum strain has photo-thermo-sensitive sterility characteristic | |
| Chandirakala et al. | Determination of fertility behaviour of thermo sensitive genic male sterile lines in rice |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20180508 |