CN105359964A - Efficient breeding method of two-line hybrid rice sterile lines - Google Patents
Efficient breeding method of two-line hybrid rice sterile lines Download PDFInfo
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- CN105359964A CN105359964A CN201510918232.XA CN201510918232A CN105359964A CN 105359964 A CN105359964 A CN 105359964A CN 201510918232 A CN201510918232 A CN 201510918232A CN 105359964 A CN105359964 A CN 105359964A
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- sterile line
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
- A01H1/04—Processes of selection involving genotypic or phenotypic markers; Methods of using phenotypic markers for selection
Abstract
The invention discloses an efficient breeding method of two-line hybrid rice sterile lines. The method of the invention comprises the following steps: 1) selection of combination/variety; 2) construction of the population; 3) preliminary screening; 4) ratoon rice stubbling: selecting proper ratoon rice clipped plant stubble period; 5) ratoon rice seed harvesting: harvesting sterile line seeds per plant during ratoon rice seed maturation, and naturally eliminating seeds which haven't been harvested; 6) single plant optimization and stabilization; 7) primary evaluation of fertility: setting three seeding periods according to the local climate; and detailing heading period and pollen fertility conditions of o sterile lines and contrast in each period; and 8) fertility evaluation: further evaluating and screening the preliminarily screened two-line sterile lines with low starting point conversion temperature, and screening two-line new sterile lines with low starting point conversion temperature. By selecting two-line sterile lines of hybrid progenies, understanding and artificial hybridization processes of resources can be omitted, and breeding efficiency can be greatly raised. The hybrid progeny optimization technology is closer to production actuality, breeding value is higher, and application value is greater.
Description
Technical field
The invention belongs to a kind of rice sterile line selection, being specifically related to a kind of two is the efficient selection of rice male sterile line.
Background technology
The discovery of paddy rice photosensitive-sensitive sterile line Nongken 58S and breeding utilization, started the New Times of double-hybrid rice strains breeding.Double-linear hybrid rice has four aspect advantages compared with ternary hybrid rice: 1. phto-temperature reaction is recessive core base, does not limit by Restore gene, and recovery spectrum is wide, combo is free; 2. the temperature sensitive sterile gene of light is genic male sterile gene, does not affect by plasmon, the negative effect of acellular mass effect; 3. phto-temperature reaction is generally main effect recessive gene, easily imports in other genetic backgrounds, and material improvement is convenient; 4. two-line sterile line one is dual-purpose, and by self propagated, breeding is easier.
But researcher is also a process deepened continuously to the understanding of photo-thermo-sensitive genetic male sterile line.The initial stage scientist understanding temperature sensitive to light also only rests on photosensitive aspect, little to temperature sensitive consideration.Until the In Middle And Lower Reaches of Changjiang River eighties in last century abnormal low temperature in midsummer causes domestic large-area double-linear hybrid rice production of hybrid seeds failure at that time, two-line sterile line of rice is caused also to develop into the two-line sterile line of rice new frame based on temperature-sensitive sterile line by paddy rice photosensitive-sensitive sterile line originally, the fertility standard that it is also proposed practical two-line sterile line is that seed selection critical temperature of sterility is lower (lower than 23.5 DEG C, be beneficial to production of hybrid seeds safety), the male sterile line of light temperature complementation comparatively strong (being beneficial to sterile line propagation).
Rice thermosensitive genie male sterile refers to its fertility change mainly by the impact of ear differentiation ambient temperature in period, and the position that rice thermosensitive genie male sterile experiences variations in temperature is young fringe, and when ambient temperature is lower than its critical temperature of sterility, after its heading, performance can be educated; When ambient temperature is higher than its critical temperature of sterility, during heading, show as pollen abortion.Therefore two-line sterile line of rice critical temperature of sterility is most important for the double-linear hybrid rice production of hybrid seeds, only have and utilize low critical temperature of sterility lower than the sterile lines seed making of 23.5 DEG C, the safe production of hybrid seeds of guarantee, so critical temperature of sterility is considered as a standard of cultivating excellent two-line sterile line of rice lower than 23.5 DEG C by breeding man.Current breeding utilizes modern large-scale constant temperature water cooling pond, critical temperature of sterility qualification is carried out to the middle sterile line material in two-line sterile line Breeding Process, cold water Selective Pressure is set, eliminate critical temperature of sterility early higher than the sterile strain of 23.5 DEG C, and then seed selection Comprehensive Traits is excellent, the two-line sterile line of rice that critical temperature of sterility is low and the production of hybrid seeds is safe.
Modern large-scale constant temperature water cooling pond is utilized also to there are some shortcomings for male sterile line qualification: 1. large-scale constant temperature water cooling pond cost is high, and technical sophistication, needs substantial contribution to drop into; 2. large-scale constant temperature water cooling pond qualification needs to select optimum detection phase and resettlement plant to detect, and labour intensity is large; 3. the qualification of two-line sterile line belongs to disposable qualification male sterile line strain, can not get rid of the fertility of fertility drift.
Summary of the invention
For above-mentioned the deficiencies in the prior art part, the invention provides and a kind ofly shorten breeding cycle, avoid two of fertility drift effect to be the efficient selection of rice male sterile line.
In order to solve the problems of the technologies described above, present invention employs following technical scheme:
A kind of two is the efficient selection of rice male sterile line, and the method comprises the steps:
1) combination/kind is selected: according to the hereditary capacity of Agronomic Traits in Rice, select Y58s, C815s that male sterile line fertility starting point conversion temperature is low, dark 08s strain leaf proterties is excellent, fecundity good, rice quality is excellent, comprehensive resistance is good two is rice combination/kind, menu strain sowing;
2) informative population: build the large segregation population of two-line sterile line, received single-strain seed by upper season and sows in mid or late March, planting density 10,000 nests/mu, this plantation single, and group size controls about 500 nests;
3) Preliminary screening: using bag neck as male sterile line mark property, screening strain Leaf pattern is good, close the male sterile line individual plant that grain husk is effective, out-crossing rate good, comprehensive resistance good, breeding time is suitable, listing mark;
4) ratoon rice stays stake: according to local ratoon rice temperature conditions in booting stage in season, select suitable ratoon rice to cut strain and stay a phase, low altitude area Pingba area is generally at 20-25 in August day Preliminary screening male sterile line individual plant, stay low level stake, vertical card mark, cut off the strain of periphery rice together simultaneously, carry out field liquid manure management;
5) ratoon rice sowing: during ratoon rice seed maturity, receives male-sterile seed by individual plant, can not receive the natural selection of seed;
6) individual plant is preferably stablized: in F3 ~ F4 generation, can utilize the high temperature in southern numerous and next year in positive season then respectively, microthermal climate carries out the screening of further individual plant and ratoon rice sowing, carry out further preferably with stable to male sterile line;
7) fertility is just identified: according to local climate situation, 3 sowing time are set, Pingba area is set to sowing time: mid-March, early April, 3 sowing time of late April, selects male sterile line C815s, the Y58s of the low starting point conversion temperature of 3 different growing, dark 08s is contrast; Heading stage of the male sterile line of each issue and contrast, pollen fertility situation are recorded in detail;
8) Fertility identification: according to step 7) method, further qualification and screening are carried out to the two-line sterile line of the low starting point conversion temperature of Preliminary screening; By to male sterile line to be measured with contrast breeding time of male sterile line, pollen fertility compares analysis, can evaluate the fertility origin temp of two-line sterile line, filter out low starting point conversion temperature two is new sterile line.
Compared with prior art, tool of the present invention has the following advantages:
1, existing excellent combination seed selection two-line sterile line is directly utilized to have following advantage: one is shorten breeding cycle; Two is little to the dependence of male sterile line and other resource; Three is that breeding resources enriches, and material is directed to breeding terminal material, aboundresources, and range of choice is wide; Four is according to genetic development, and start with from the excellent combination of selection, breeding efficiency is high, with strong points, good practical effect.Utilize contrast qualification to be by stages the natural appraisal under ecoclimatic condition, arrange 2 years or identify the impact that fertility can also be avoided to drift about for many years, practical value is good.
2, hybrid generation's optimization technique avoids the limitation that resource is introduced, and can get around the barrier that resource is introduced.
3, the preferred two-line sterile line of hybrid generation can save understanding and the artificial hybridization process of resource, greatly improves breeding efficiency.
4, hybrid generation's optimization technique is more pressed close to produce actual, and Breeding value is higher, and using value is larger.
Embodiment
A kind of two is the efficient selection of rice male sterile line, and the method comprises the steps:
1) combination/kind is selected: according to the hereditary capacity of Agronomic Traits in Rice, select that the strain leaf proterties of Y58s, the C815s that male sterile line fertility starting point conversion temperature is low, dark 08s two-line sterile line combo is excellent, fecundity good, rice quality is excellent, comprehensive resistance is good two is rice combination/kind, menu strain sowing;
2) informative population: build the large segregation population of two-line sterile line, received single-strain seed by upper season and sows in mid or late March, planting density 10,000 nests/mu, this plantation single, and group size controls about 500 nests;
3) Preliminary screening: using bag neck as male sterile line mark property, screening strain Leaf pattern is good, close the male sterile line individual plant that grain husk is effective, out-crossing rate good, comprehensive resistance good, breeding time is suitable, listing mark;
4) ratoon rice stays stake: according to local ratoon rice temperature conditions in booting stage in season, select suitable ratoon rice to cut strain and stay a phase, low altitude area Pingba area is generally at 20-25 in August day Preliminary screening male sterile line individual plant, stay low level stake, vertical card mark, cut off the strain of periphery rice together simultaneously, carry out field liquid manure management;
5) ratoon rice sowing: during ratoon rice seed maturity, receives male-sterile seed by individual plant, can not receive the natural selection of seed;
6) individual plant is preferably stablized: in F3 ~ F4 generation, can utilize the high temperature in southern numerous and next year in positive season then respectively, microthermal climate carries out the screening of further individual plant and ratoon rice sowing, carry out further preferably with stable to male sterile line;
7) fertility is just identified: according to local climate situation, 3 sowing time are set, Pingba area is set to sowing time: mid-March, early April, 3 sowing time of late April, selects male sterile line C815s, the Y58s of the low starting point conversion temperature of 3 different growing, dark 08s is contrast; Heading stage of the male sterile line of each issue and contrast, pollen fertility situation are recorded in detail;
8) Fertility identification: according to step 7) method, further qualification and screening are carried out to the two-line sterile line of the low starting point conversion temperature of Preliminary screening; By to male sterile line to be measured with contrast breeding time of male sterile line, pollen fertility compares analysis, can evaluate the fertility origin temp of two-line sterile line, filter out low starting point conversion temperature two is new sterile line.
The efficient selection of two-line sterile line and conventional herd breeding method comparative analysis table
What finally illustrate is, above embodiment is only in order to illustrate technical scheme of the present invention and unrestricted, although with reference to preferred embodiment to invention has been detailed description, those of ordinary skill in the art is to be understood that, can modify to technical scheme of the present invention or equivalent replacement, and not departing from aim and the scope of technical solution of the present invention, it all should be encompassed in the middle of right of the present invention.
Claims (1)
1. two is the efficient selection of rice male sterile line, and it is characterized in that, the method comprises the steps:
1) combination/kind is selected: according to the hereditary capacity of Agronomic Traits in Rice, select Y58s, C815s that male sterile line fertility starting point conversion temperature is low, dark 08s strain leaf proterties is excellent, fecundity good, rice quality is excellent, comprehensive resistance is good two is rice combination/kind, menu strain sowing;
2) informative population: build the large segregation population of two-line sterile line, received single-strain seed by upper season and sows in mid or late March, planting density 10,000 nests/mu, this plantation single, and group size controls about 500 nests;
3) Preliminary screening: using bag neck as male sterile line mark property, screening strain Leaf pattern is good, close the male sterile line individual plant that grain husk is effective, out-crossing rate good, comprehensive resistance good, breeding time is suitable, listing mark;
4) ratoon rice stays stake: according to local ratoon rice temperature conditions in booting stage in season, select suitable ratoon rice to cut strain and stay a phase, low altitude area Pingba area is generally at 20-25 in August day Preliminary screening male sterile line individual plant, stay low level stake, vertical card mark, cut off the strain of periphery rice together simultaneously, carry out field liquid manure management;
5) ratoon rice sowing: during ratoon rice seed maturity, receives male-sterile seed by individual plant, can not receive the natural selection of seed;
6) individual plant is preferably stablized: in F3 ~ F4 generation, can utilize the high temperature in southern numerous and next year in positive season then respectively, microthermal climate carries out the screening of further individual plant and ratoon rice sowing, carry out further preferably with stable to male sterile line;
7) fertility is just identified: according to local climate situation, 3 sowing time are set, Pingba area is set to sowing time: mid-March, early April, 3 sowing time of late April, selects male sterile line C815s, the Y58s of the low starting point conversion temperature of 3 different growing, dark 08s is contrast; Heading stage of the male sterile line of each issue and contrast, pollen fertility situation are recorded in detail;
8) Fertility identification: according to the method for step 7), carries out qualification further and screening to the two-line sterile line of the low starting point conversion temperature of Preliminary screening; By to male sterile line to be measured with contrast breeding time of male sterile line, pollen fertility compares analysis, can evaluate the fertility origin temp of two-line sterile line, filter out low starting point conversion temperature two is new sterile line.
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107996395A (en) * | 2018-01-31 | 2018-05-08 | 重庆市农业科学院 | A kind of selection of low critical temperature of sterility two-line sterile line rice |
| CN109618920A (en) * | 2019-02-23 | 2019-04-16 | 重庆市农业科学院 | A kind of hybrid paddy rice dual purpose genic male sterile line clonal regeneration pure keeping method |
| CN111296274A (en) * | 2020-02-21 | 2020-06-19 | 芜湖市星火农业实用技术研究所 | Rice variety breeding method of ratoon rice |
| CN112753564A (en) * | 2020-12-29 | 2021-05-07 | 湖南省春云农业科技股份有限公司 | Breeding method of high-quality two-line sterile line of rice |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1337148A (en) * | 2001-09-24 | 2002-02-27 | 华中农业大学 | Breeding method of overcoming the problem of raised critical sterile temperature of two-purpose rice sterile line |
| CN104770291A (en) * | 2015-04-20 | 2015-07-15 | 中国水稻研究所 | Selective breeding method for two-line paddy sterility line with low sterile critical temperature |
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- 2015-12-10 CN CN201510918232.XA patent/CN105359964B/en active Active
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1337148A (en) * | 2001-09-24 | 2002-02-27 | 华中农业大学 | Breeding method of overcoming the problem of raised critical sterile temperature of two-purpose rice sterile line |
| CN104770291A (en) * | 2015-04-20 | 2015-07-15 | 中国水稻研究所 | Selective breeding method for two-line paddy sterility line with low sterile critical temperature |
Non-Patent Citations (1)
| Title |
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| 李成荃: "《安徽稻作学》", 31 March 2008 * |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107996395A (en) * | 2018-01-31 | 2018-05-08 | 重庆市农业科学院 | A kind of selection of low critical temperature of sterility two-line sterile line rice |
| CN109618920A (en) * | 2019-02-23 | 2019-04-16 | 重庆市农业科学院 | A kind of hybrid paddy rice dual purpose genic male sterile line clonal regeneration pure keeping method |
| CN111296274A (en) * | 2020-02-21 | 2020-06-19 | 芜湖市星火农业实用技术研究所 | Rice variety breeding method of ratoon rice |
| CN112753564A (en) * | 2020-12-29 | 2021-05-07 | 湖南省春云农业科技股份有限公司 | Breeding method of high-quality two-line sterile line of rice |
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