CN107996278A - A kind of method of hazel tree seed seedling-raising inoculation India truffle - Google Patents
A kind of method of hazel tree seed seedling-raising inoculation India truffle Download PDFInfo
- Publication number
- CN107996278A CN107996278A CN201711225439.4A CN201711225439A CN107996278A CN 107996278 A CN107996278 A CN 107996278A CN 201711225439 A CN201711225439 A CN 201711225439A CN 107996278 A CN107996278 A CN 107996278A
- Authority
- CN
- China
- Prior art keywords
- seedling
- hazel tree
- inoculation
- india truffle
- seed
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
Abstract
The present invention discloses a kind of hazel tree seed and is configuring the inoculation method in India truffle matrix soil, belongs to wild-type strain planting technology field.The pretreatment that carries out disinfection first to hazel tree seed is spare, selection is associated with the hazel tree mycorhiza of ferfas and fresh India truffle crush after be equipped with sterile water and obtain truffle mycorrhiza fungi liquid and strain liquid, take river sand, vermiculite, virgin soil soil to mix and add India truffle strain liquid and adjust moisture content by appropriate mass ratio and obtain that seedling medium is spare, take river sand, vermiculite, virgin soil soil, mycorhiza soil to mix by appropriate mass ratio and adjust moisture content to obtain inoculation matrix spare；Then sowed in greenhouse in seedling medium, to seed sprouting after 12 months, taking-up be immersed at least 3.5min in India truffle strain liquid, transplant again in implantation inoculation matrix, illuminance, temperature and humidity in greenhouse are controlled, seedling management glue, weeding is carried out, grows up to mycorhiza sapling to seedling.There is method is reliable, rate of vaccination is high, cost is low etc..
Description
Technical field
The present invention relates to a kind of India truffle inoculation method, especially a kind of hazel tree seed seedling-raising inoculation India truffle
Method, belongs to wild-type strain planting technology field.
Background technology
India truffle (Tuber indicum), also known as truffle, popular name pig encircle bacterium, without ma fruit etc., are referred to as " the world first
Name bacterium ".India truffle is rich in 17 kinds of amino acid, 8 kinds of vitamins, suitable protein and androsterone, sterol, sphingolipid, fat
Acid, amino acid and trace element etc. more than 50 plants physiologically active ingredient, and cannot be synthesized containing human body itself 8 kinds of amino acid, zinc,
The essential nutrients such as manganese, iron, calcium, phosphorus, selenium, with medicinal valencys such as strengthen immunity, anti-aging, beneficial stomach, clear god, hemostasis, treatment hemorrhoid
Value, has active anticancer, there is certain inhibitory action to cancer cell, can excite brain cell activity.Due to wild India truffle
Low output by artificial cultivation, it is necessary to meet ever-increasing market demand, and effective inoculation method is not only India's block
The premise of bacterium Mycorrhizal production, and the key of ferfas cultivation success or failure.
Hazel tree（Corylus heterophylla Fisch）, also known as fibert, is four big dry fruit in the world（It is walnut, flat
Peach, fibert, cashew nut）One of, it is Betulaceae Corylus shrub or dungarunga, is born in the mountainous region Schattenseite shrubbery of 200-1000 meters of height above sea level
In.Be distributed in China, Turkey, Italy, Spain, the U.S., Korea, Japan, Russia East Siberia and the Far East Area,
Mongolian east, the country are distributed in Guizhou, STRUCTURES IN EAST SICHUAN, Shaanxi, Middle Gansu Province and the southeast, Henan, Shandong, Jiangsu, Anhui, Zhejiang
The ground such as river, Jiangxi, are born in the mountainous region woodland of 700-2500 meters of height above sea level, and winter resistance is strong, like the weather of moistening.It more enjoys light,
Sufficient illumination can promote its growth development and result.The modes of reproduction of fibert sapling has a seeding and seedling raising, plant division, root turion nursery and
Layering seedling growth, Seed germinating ability are kept for 1 year, and the sowing time is advisable with spring.
At present, though having the cultivation inoculation technique on India truffle on a small quantity both at home and abroad, and apply to a certain extent manually
In cultivation practices, but rest on the exploratory stage, technology is also remote immature more.Hazel tree as India truffle host plant it
One, attract attention in the artificial cultivation of India truffle, but existing hazel tree inoculation technique is still immature, and effect of inoculation is paid no attention to
Think, success ratio of inoculation is relatively low, causes the cost increase of India truffle cultivation, yield to be limited, it is difficult to promote and apply, it is necessary to study
The Huashan pine India truffle Inoculation Method of effect.
The content of the invention
The technical problem to be solved in the present invention is low, of high cost for success ratio of inoculation in existing India truffle artificial cultivation
The deficiencies of, propose a kind of method of hazel tree seed seedling-raising inoculation India truffle, by host plant seedling culture and build bacterium
Kind growing environment, improves India truffle success ratio of inoculation, reduces production cost.
In order to solve the above technical problems, the present invention proposes a kind of method of hazel tree seed seedling-raising inoculation India truffle, its
Comprise the following steps that：
（1）Seeds preprocess：Hazel tree seed is taken, is placed at 0-4 DEG C in refrigerator preservation 4-5 months, in aseptic condition after taking-up
It is lower to be disinfected three times, first with the H of concentration 35-40%2O2When liquid immersion 0.5-1 is small, done after scouring with aseptic water washing
Only；Then it is put into the ethanol of concentration 30-35% and carries out disinfection 10-20min for the second time, it is clean with aseptic water washing；Place into dense
Spend in the aqueous sodium hypochlorite solution of 1.5-5% and carry out third time disinfection 15-25min；Finally use aseptic water washing 2-3 times, obtain pre-
Fibert seed after processing, is placed in spare in culture dish；
（2）Prepare ferfas strain liquid：Ripe fresh India truffle is selected, aseptic water washing is used after cleaning up 2-3 times, is then put
The smalls shape of granularity 50-80 mesh is crushed in pulverizer, then by 1：The volume ratio of 5-10 adds sterile water, obtains India truffle
Strain liquid is spare；
（3）Prepare seedling medium：By 1：0.8-1：The weight ratio of 3-4, takes dry river sand, vermiculite, virgin soil soil to be crushed to granularity
Less than or equal to 4mm and it is uniformly mixed, adds step（2）The truffle mycorrhiza fungi liquid of preparation, makes mixture moisture content reach 50-55%,
It is spare to obtain seedling medium；
（4）Prepare inoculation matrix：By 1：0.8-1：2-3：The weight ratio of 2-3, takes dry river sand, vermiculite, virgin soil soil, hazel tree
Mycorhiza soil is crushed to granularity less than or equal to 4mm and is uniformly mixed, and admixing water in right amount makes moisture content reach 40-45%, obtains inoculation base
Matter is spare；
（5）Seeding and seedling raising：In greenhouse, appropriate temperature, humidity, illuminance are controlled, by step（1）In it is pretreated
Hazel tree seed, step is sown into by the depth of 3-5cm（3）In obtained seedling medium, then seedling management is carried out according to a conventional method,
Watering, weeding, keep the humidity of seedling medium, to seed sprouting after 1-2 months, obtain hazel tree seedling；
（6）Mycorrhiza fungi seeding cultivating：Step is taken out from seedling medium（5）Gained hazel tree seedling, the root system of hazel tree seedling is soaked
Enter step（2）At least 3.5min in the India truffle strain liquid of preparation, implantation step after taking-up（4）In gained inoculation matrix, control
Appropriate temperature, humidity, illuminance in greenhouse processed, carry out seedling management according to a conventional method, water, weeding, and monthly right
Seedling applies step（2）The ferfas strain liquid of preparation 2-3 times, keeps the humidity of inoculation matrix, through the cultivation of 4-6 months, that is, obtains
India truffle mycorrhiza fungi seeding.
The hazel tree seed is the single plant hazel that yield is high, grain is big, benevolence is full, no disease and pests harm, continuous bearing capacity are strong
The seed of subtree.
The step（3）With（4）In, adjustment seedling medium and be inoculated with matrix pH value be 6.7-7.5, be it is slightly sour or in
Property, in favor of the growth of the emergence of hazel tree seed, growth and strain.
The step（4）In, the hazel tree mycorhiza soil for preparing inoculation matrix is taken from being associated with India's block in the fibert woods
Loose topsoil under the hazel tree of bacterium, with ensure strain inoculation after well-grown, avoid nursery lack pine tree mycorhiza soil and
Influence the growth of inoculation strain.
The step（5）In, after seed germination, monthly step of applying again（2）The ferfas strain liquid of preparation 2-3 times, to increase
The effect of inoculation of strong India truffle.
The step（5）In, the density of hazel tree seedling implantation inoculation matrix is every square metre 150-300 plants.
The step（5）In, sowing time of hazel tree seed preferably in the annual 4-5 months, in favor of seed sprouting and tree
The growth of seedling, is advisable with mid or late April.
The step（5）With（6）In, to control the temperature in greenhouse be 20-26 DEG C, humidity 75-85%, illuminance
For 20000-28000lux.
The composition proportion of seedling medium and inoculation matrix, inoculation soil humidity, seedling cultivation density, greenhouse are big in the present invention
Each parameter such as the temperature and humidity in canopy can be specific definite according to being actually needed, and ensures good effect of inoculation.
The present invention is connect using the seedling medium with India truffle strain and appropriate component, inoculation matrix using special
Kind method for culturing seedlings, by the multiple strain charity of too many levels, for India truffle strain, in the place of fibert sapling root, life is built
Good environment, substantially increases the success rate of inoculation, reduces the cost of artificial growth India truffle, can make India truffle
Rate of vaccination reaches more than 90%.4-7 enters harvest time, more than 8kg/ mus of per unit area yield after mycorhiza transplantation of seedlings plantation.Can with method
Lean on, the advantage such as rate of vaccination is high, cost is low, be suitably widely popularized use.
Embodiment
The embodiment of the present invention is further described in detail below.The technology being not specified in embodiment or production
Product are the prior art or can be by buying the conventional products obtained.
Embodiment 1：The method of this hazel tree seed seedling-raising inoculation India truffle comprises the following steps that：
（1）Seeds preprocess：Take the single plant hazel tree that yield is high, grain is big, benevolence is full, no disease and pests harm, continuous bearing capacity are strong
Seed, be placed at 0 DEG C preservation 4 months in refrigerator, aseptically disinfected three times after taking-up, first use concentration
35% H2O2It is clean with aseptic water washing after scouring when liquid immersion 1 is small；Then it is put into the ethanol of concentration 30% and carries out second
Secondary disinfection 20min is clean with aseptic water washing；Place into progress third time disinfection in the aqueous sodium hypochlorite solution of concentration 1.5%
25min；Finally use aseptic water washing 2 times, obtain pretreated fibert seed, be placed in spare in culture dish；
（2）Prepare ferfas strain liquid：Ripe fresh India truffle is selected, aseptic water washing is used after cleaning up 2 times, is subsequently placed in
The smalls shape of 50 mesh of granularity is crushed in pulverizer, then by 1：10 volume ratio adds sterile water, obtains India truffle strain liquid
It is spare；
（3）Prepare seedling medium：By 1：0.8：3 weight ratio, take dry river sand, vermiculite, virgin soil soil be crushed to granularity 2mm with
Descend and be uniformly mixed, add step（2）The truffle mycorrhiza fungi liquid of preparation, makes mixture moisture content adjust seedling medium up to 50%
PH value be 6.7, it is spare to obtain seedling medium；
（4）Prepare inoculation matrix：By 1：0.8：2：2 weight ratio, takes dry river sand, vermiculite, virgin soil soil, hazel tree mycorhiza soil
It is crushed to below granularity 2mm and is uniformly mixed, admixing water in right amount makes moisture content reach 40%, and the pH value for adjusting seedling medium is
6.7, it is spare to obtain inoculation matrix；Hazel tree mycorhiza soil used is taken from being associated with the hazel tree of India truffle in the fibert woods
Loose topsoil, to ensure well-grown after strain inoculation；
（5）Seeding and seedling raising：Annual late April, in greenhouse, 20 DEG C of temperature of shed of control, humidity 75%, illuminance
20000lux, by step（1）In pretreated hazel tree seed, be sown into by 150 plants every square metre of density and the depth of 3cm
Step（3）In obtained seedling medium, then seedling management is carried out according to a conventional method, watering, weeding, monthly pour and apply step again after emergence
Suddenly（2）The ferfas strain liquid of preparation 2 times, keep seedling medium 50% moisture content, to seed sprouting after 1 month, obtain fibert
Set seedling；
（6）Mycorrhiza fungi seeding cultivating：Step is taken out from seedling medium（5）Gained hazel tree seedling, the root system of hazel tree seedling is soaked
Enter step（2）3.5min in the India truffle strain liquid of preparation, implantation step after taking-up（4）In gained inoculation matrix, control temperature
20 DEG C of room canopy temperature, humidity 75%, illuminance 20000lux, carry out seedling management according to a conventional method, water, weeding, and often
The moon applies step to seedling（2）The ferfas strain liquid of preparation 2 times, keeps the moisture content of inoculation matrix 40%, through the cultivation of 4 months, i.e.,
Obtain India truffle mycorrhiza fungi seeding.
For the present embodiment by using the method for the present invention, the rate of vaccination of India truffle is 90%, enters harvest within the 7th year after plantation
Phase, the per unit area yield of ferfas brings up to 8kg/ mus by the 2kg/ mus of existing method, and ferfas promotes the growth and development of masson pine, at the same time
Hazel tree effectively promotes the diseases and insect pests resistance of the growth and development raising plant of ferfas again, can keep and stablize ecosystem
System, promotes the recovery and development of forest cover.
Embodiment 2：The method of this hazel tree seed seedling-raising inoculation India truffle comprises the following steps that：
（1）Seeds preprocess：Take the single plant hazel tree that yield is high, grain is big, benevolence is full, no disease and pests harm, continuous bearing capacity are strong
Seed, be placed at 4 DEG C preservation 5 months in refrigerator, aseptically disinfected three times after taking-up, first use concentration
40% H2O2It is clean with aseptic water washing after scouring when liquid immersion 0.5 is small；Then it is put into the ethanol of concentration 35% and carries out
Secondary sterilization 10min is clean with aseptic water washing；Place into progress third time disinfection in the aqueous sodium hypochlorite solution of concentration 5%
15min；Finally use aseptic water washing 3 times, obtain pretreated fibert seed, be placed in spare in culture dish；
（2）Prepare ferfas strain liquid：Ripe fresh India truffle is selected, aseptic water washing is used after cleaning up 3 times, is subsequently placed in
The smalls shape of 50 mesh of granularity is crushed in pulverizer, then by 1：5 volume ratio adds sterile water, and it is standby to obtain India truffle strain liquid
With；
（3）Prepare seedling medium：By 1：1：4 weight ratio, takes dry river sand, vermiculite, virgin soil soil to be crushed to granularity 4mm and mixes
Close uniformly, add step（2）The truffle mycorrhiza fungi liquid of preparation, makes mixture moisture content up to 55%, adjusts the pH value of seedling medium
For 7.5, it is spare to obtain seedling medium；
（4）Prepare inoculation matrix：By 1：1：3：3 weight ratio, takes dry river sand, vermiculite, virgin soil soil, hazel tree mycorhiza soil powder
It is broken to granularity 4mm and is uniformly mixed, admixing water in right amount makes moisture content reach 45%, and the pH value for adjusting seedling medium is 7.5, is obtained
It is spare to be inoculated with matrix；Hazel tree mycorhiza soil used is taken from being associated with table loose under the hazel tree of India truffle in the fibert woods
Layer soil, to ensure well-grown after strain inoculation；
（5）Seeding and seedling raising：Annual 4 the middle ten days month, in greenhouse, 26 DEG C of temperature of shed of control, humidity 85%, illuminance
28000lux, by step（1）In pretreated hazel tree seed, be sown into by 300 plants every square metre of density and the depth of 5cm
Step（3）In obtained seedling medium, then seedling management is carried out according to a conventional method, watering, weeding, every month pours again after emergence applies
Step（2）The ferfas strain liquid of preparation 3 times, keep seedling medium 55% moisture content, to seed sprouting after 2 months, obtain hazel
Subtree seedling；
（6）Mycorrhiza fungi seeding cultivating：Step is taken out from seedling medium（5）Gained hazel tree seedling, the root system of hazel tree seedling is soaked
Enter step（2）10min in the India truffle strain liquid of preparation, implantation step after taking-up（4）In gained inoculation matrix, greenhouse is controlled
26 DEG C of canopy temperature, humidity 85%, illuminance 28000lux, carry out seedling management according to a conventional method, water, weeding, and monthly
Step is applied to seedling（2）The ferfas strain liquid of preparation 3 times, keeps the moisture content of inoculation matrix 45%, through the cultivation of 6 months, to obtain the final product
To India truffle mycorrhiza fungi seeding.
For the present embodiment by using the method for the present invention, the rate of vaccination of India truffle is 95%, enters harvest within the 4th year after plantation
Phase, the per unit area yield of ferfas brings up to 12kg/ mus by the 2kg/ mus of existing method, and ferfas promotes the growth and development of masson pine, together
When hazel tree effectively promote again ferfas growth and development improve plant diseases and insect pests resistance, can keep and stablize ecosystem
System, promotes the recovery and development of forest cover.
Embodiment 3：The method of this hazel tree seed seedling-raising inoculation India truffle comprises the following steps that：
（1）Seeds preprocess：Take the single plant hazel tree that yield is high, grain is big, benevolence is full, no disease and pests harm, continuous bearing capacity are strong
Seed, be placed at 2 DEG C preservation 4.5 months in refrigerator, aseptically disinfected three times after taking-up, first use concentration
38% H2O2It is clean with aseptic water washing after scouring when liquid immersion 0.6 is small；Then it is put into the ethanol of concentration 32% and carries out
Secondary sterilization 15min is clean with aseptic water washing；Place into progress third time disinfection in the aqueous sodium hypochlorite solution of concentration 3%
20min；Finally use aseptic water washing 3 times, obtain pretreated fibert seed, be placed in spare in culture dish；
（2）Prepare ferfas strain liquid：Ripe fresh India truffle is selected, aseptic water washing is used after cleaning up 3 times, is subsequently placed in
The smalls shape of 80 mesh of granularity is crushed in pulverizer, then by 1：8 volume ratio adds sterile water, and it is standby to obtain India truffle strain liquid
With；
（3）Prepare seedling medium：By 1：0.9：3.5 weight ratio, takes dry river sand, vermiculite, virgin soil soil to be crushed to granularity 3mm
Below and it is uniformly mixed, adds step（2）The truffle mycorrhiza fungi liquid of preparation, makes mixture moisture content adjust nursery base up to 52%
The pH value of matter is 7.0, and it is spare to obtain seedling medium；
（4）Prepare inoculation matrix：By 1：1：2.5：2.5 weight ratio, takes dry river sand, vermiculite, virgin soil soil, hazel tree mycorhiza
Soil is crushed to below granularity 3mm and is uniformly mixed, and admixing water in right amount makes moisture content reach 42%, and the pH value for adjusting seedling medium is
7.0, it is spare to obtain inoculation matrix；Hazel tree mycorhiza soil used is taken from being associated with the hazel tree of India truffle in the fibert woods
Loose topsoil, to ensure well-grown after strain inoculation；
（5）Seeding and seedling raising：Annual mid-April, in greenhouse, 23 DEG C of temperature of shed of control, humidity 80%, illuminance
25000lux, by step（1）In pretreated hazel tree seed, be sown into by 200 plants every square metre of density and the depth of 4cm
Step（3）In obtained seedling medium, then seedling management is carried out according to a conventional method, watering, weeding, every month pours again after emergence applies
Step（2）The ferfas strain liquid of preparation 2 times, keep seedling medium 52% moisture content, to seed sprouting after 1 month, obtain hazel
Subtree seedling；
（6）Mycorrhiza fungi seeding cultivating：Step is taken out from seedling medium（5）Gained hazel tree seedling, the root system of hazel tree seedling is soaked
Enter step（2）7min in the India truffle strain liquid of preparation, implantation step after taking-up（4）In gained inoculation matrix, greenhouse is controlled
23 DEG C of canopy temperature, humidity 80%, illuminance 25000lux, carry out seedling management according to a conventional method, water, weeding, and monthly
Step is applied to seedling（2）The ferfas strain liquid of preparation 3 times, keeps the moisture content of inoculation matrix 42%, through the cultivation of 5 months, to obtain the final product
To India truffle mycorrhiza fungi seeding.
For the present embodiment by using the method for the present invention, the rate of vaccination of India truffle is 91%, enters harvest within the 6th year after plantation
Phase, the per unit area yield of ferfas brings up to 9kg/ mus by the 2kg/ mus of existing method, and ferfas promotes the growth and development of masson pine, at the same time
Hazel tree effectively promotes the diseases and insect pests resistance of the growth and development raising plant of ferfas again, can keep and stablize ecosystem
System, promotes the recovery and development of forest cover.
Embodiment 4：The method of this hazel tree seed seedling-raising inoculation India truffle comprises the following steps that：
（1）Seeds preprocess：Take the single plant hazel tree that yield is high, grain is big, benevolence is full, no disease and pests harm, continuous bearing capacity are strong
Seed, be placed at 1 DEG C preservation 5 months in refrigerator, aseptically disinfected three times after taking-up, first use concentration
36% H2O2It is clean with aseptic water washing after scouring when liquid immersion 0.6 is small；Then it is put into the ethanol of concentration 33% and carries out
Secondary sterilization 13min is clean with aseptic water washing；Place into progress third time disinfection in the aqueous sodium hypochlorite solution of concentration 2%
18min；Finally use aseptic water washing 2 times, obtain pretreated fibert seed, be placed in spare in culture dish；
（2）Prepare ferfas strain liquid：Ripe fresh India truffle is selected, aseptic water washing is used after cleaning up 2 times, is subsequently placed in
The smalls shape of 60 mesh of granularity is crushed in pulverizer, then by 1：6 volume ratio adds sterile water, and it is standby to obtain India truffle strain liquid
With；
（3）Prepare seedling medium：By 1：0.9：4 weight ratio, takes dry river sand, vermiculite, virgin soil soil to be crushed to granularity 3.5mm
Below and it is uniformly mixed, adds step（2）The truffle mycorrhiza fungi liquid of preparation, makes mixture moisture content adjust nursery base up to 54%
The pH value of matter is 6.9, and it is spare to obtain seedling medium；
（4）Prepare inoculation matrix：By 1：0.8：3：3 weight ratio, takes dry river sand, vermiculite, virgin soil soil, hazel tree mycorhiza soil
It is crushed to below granularity 3mm and is uniformly mixed, admixing water in right amount makes moisture content reach 43%, and the pH value for adjusting seedling medium is
6.9, it is spare to obtain inoculation matrix；Hazel tree mycorhiza soil used is taken from being associated with the hazel tree of India truffle in the fibert woods
Loose topsoil, to ensure well-grown after strain inoculation；
（5）Seeding and seedling raising：At the beginning of annual 5 months, in greenhouse, 25 DEG C of temperature of shed of control, humidity 78%, illuminance
23000lux, by step（1）In pretreated hazel tree seed, broadcast by 180 plants every square metre of density and the depth of 3.5cm
Enter step（3）In obtained seedling medium, then carry out seedling management according to a conventional method, watering, weeding, after emergence every month pour again
Apply step（2）The ferfas strain liquid of preparation 3 times, keep seedling medium 55% moisture content, to seed sprouting after 2 months, obtain
Hazel tree seedling；
（6）Mycorrhiza fungi seeding cultivating：Step is taken out from seedling medium（5）Gained hazel tree seedling, the root system of hazel tree seedling is soaked
Enter step（2）8min in the India truffle strain liquid of preparation, implantation step after taking-up（4）In gained inoculation matrix, greenhouse is controlled
25 DEG C of canopy temperature, humidity 78%, illuminance 23000lux, carry out seedling management according to a conventional method, water, weeding, and monthly
Step is applied to seedling（2）The ferfas strain liquid of preparation 3 times, keeps the moisture content of inoculation matrix 45%, through the cultivation of 5 months, to obtain the final product
To India truffle mycorrhiza fungi seeding.
For the present embodiment by using the method for the present invention, the rate of vaccination of India truffle is 93%, enters harvest within the 5th year after plantation
Phase, the per unit area yield of ferfas brings up to 11kg/ mus by the 2kg/ mus of existing method, and ferfas promotes the growth and development of masson pine, together
When hazel tree effectively promote again ferfas growth and development improve plant diseases and insect pests resistance, can keep and stablize ecosystem
System, promotes the recovery and development of forest cover.
The technology contents of the present invention are described above, but protection scope of the present invention is not limited to the content,
, can also be on the premise of present inventive concept not be departed to this hair in the knowledge that those of ordinary skill in the art possess
Bright technology contents make a variety of changes, within the spirit and principles of the invention, any modification for being made, equivalent substitution,
Improve etc., it should all be included in the protection scope of the present invention.
Claims (8)
- A kind of 1. method of hazel tree seed seedling-raising inoculation India truffle, it is characterised in that comprise the following steps that：（1）Seeds preprocess：Hazel tree seed is taken, is placed at 0-4 DEG C in refrigerator preservation 4-5 months, in aseptic condition after taking-up It is lower to be disinfected three times, first with the H of concentration 35-40%2O2When liquid immersion 0.5-1 is small, done after scouring with aseptic water washing Only；Then it is put into the ethanol of concentration 30-35% and carries out disinfection 10-20min for the second time, it is clean with aseptic water washing；Place into dense Spend in the aqueous sodium hypochlorite solution of 1.5-5% and carry out third time disinfection 15-25min；Finally use aseptic water washing 2-3 times, obtain pre- Fibert seed after processing, is placed in spare in culture dish；（2）Prepare ferfas strain liquid：Ripe fresh India truffle is selected, aseptic water washing is used after cleaning up 2-3 times, is then put The smalls shape of granularity 50-80 mesh is crushed in pulverizer, then by 1：The volume ratio of 5-10 adds sterile water, obtains India truffle Strain liquid is spare；（3）Prepare seedling medium：By 1：0.8-1：The weight ratio of 3-4, takes dry river sand, vermiculite, virgin soil soil to be crushed to granularity Less than or equal to 4mm and it is uniformly mixed, adds step（2）The truffle mycorrhiza fungi liquid of preparation, makes mixture moisture content reach 50-55%, It is spare to obtain seedling medium；（4）Prepare inoculation matrix：By 1：0.8-1：2-3：The weight ratio of 2-3, takes dry river sand, vermiculite, virgin soil soil, hazel tree Mycorhiza soil is crushed to granularity less than or equal to 4mm and is uniformly mixed, and admixing water in right amount makes moisture content reach 40-45%, obtains inoculation base Matter is spare；（5）Seeding and seedling raising：In greenhouse, appropriate temperature, humidity, illuminance are controlled, by step（1）In it is pretreated Hazel tree seed, step is sown into by the depth of 3-5cm（3）In obtained seedling medium, then seedling management is carried out according to a conventional method, Watering, weeding, keep the humidity of seedling medium, to seed sprouting after 1-2 months, obtain hazel tree seedling；（6）Mycorrhiza fungi seeding cultivating：Step is taken out from seedling medium（5）Gained hazel tree seedling, the root system of hazel tree seedling is soaked Enter step（2）At least 3.5min in the India truffle strain liquid of preparation, implantation step after taking-up（4）In gained inoculation matrix, control Appropriate temperature, humidity, illuminance in greenhouse processed, carry out seedling management according to a conventional method, water, weeding, and monthly right Seedling applies step（2）The ferfas strain liquid of preparation 2-3 times, keeps the humidity of inoculation matrix, through the cultivation of 4-6 months, that is, obtains India truffle mycorrhiza fungi seeding.
- 2. the method for hazel tree seed seedling-raising inoculation India truffle according to claim 1, it is characterised in that：The fibert Seeds is the seed for the single plant hazel tree that yield is high, grain is big, benevolence is full, no disease and pests harm, continuous bearing capacity are strong.
- 3. the method for hazel tree seed seedling-raising inoculation India truffle according to claim 1, it is characterised in that：The step （3）With（4）In, adjustment seedling medium and the pH value for being inoculated with matrix are 6.7-7.5.
- 4. the method for hazel tree seed seedling-raising inoculation India truffle according to claim 1, it is characterised in that：The step （4）In, the hazel tree mycorhiza soil for preparing inoculation matrix is taken from being associated with the fibert woods under the hazel tree of India truffle loosely Topsoil.
- 5. the method for hazel tree seed seedling-raising inoculation India truffle according to claim 1, it is characterised in that：The step （5）In, after seed germination, monthly step of applying again（2）The ferfas strain liquid of preparation 2-3 times.
- 6. the method for hazel tree seed seedling-raising inoculation India truffle according to claim 1, it is characterised in that：The step （5）In, the density of hazel tree seedling implantation inoculation matrix is every square metre 150-300 plants.
- 7. the method for hazel tree seed seedling-raising inoculation India truffle according to claim 1, it is characterised in that：The step （5）In, the sowing time of hazel tree seed is preferably in the annual 4-5 months.
- 8. the method for hazel tree seed seedling-raising inoculation India truffle according to claim 1, it is characterised in that：The step （5）With（6）In, to control the temperature in greenhouse be 20-26 DEG C, humidity 75-85%, illuminance 20000-28000lux.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201711225439.4A CN107996278A (en) | 2017-11-29 | 2017-11-29 | A kind of method of hazel tree seed seedling-raising inoculation India truffle |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201711225439.4A CN107996278A (en) | 2017-11-29 | 2017-11-29 | A kind of method of hazel tree seed seedling-raising inoculation India truffle |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN107996278A true CN107996278A (en) | 2018-05-08 |
Family
ID=62054748
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201711225439.4A Pending CN107996278A (en) | 2017-11-29 | 2017-11-29 | A kind of method of hazel tree seed seedling-raising inoculation India truffle |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN107996278A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN117652343A (en) * | 2024-01-31 | 2024-03-08 | 云南上智科技有限公司 | Mycorrhiza synthesis method of target plant |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101061781A (en) * | 2007-04-23 | 2007-10-31 | 攀枝花市农林科学研究院 | Chinese truffle and India truffle mycorrhiza fungi seeding cultivating method |
| CN101328464A (en) * | 2008-05-27 | 2008-12-24 | 中国科学院昆明植物研究所 | Synthetic method of truffle and bacteriorhiza |
-
2017
- 2017-11-29 CN CN201711225439.4A patent/CN107996278A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101061781A (en) * | 2007-04-23 | 2007-10-31 | 攀枝花市农林科学研究院 | Chinese truffle and India truffle mycorrhiza fungi seeding cultivating method |
| CN101328464A (en) * | 2008-05-27 | 2008-12-24 | 中国科学院昆明植物研究所 | Synthetic method of truffle and bacteriorhiza |
Non-Patent Citations (1)
| Title |
|---|
| 耿丽英: "板栗树种子育苗接种印度块菌的方法", 《印度块根与华山松、板栗和锥连栎的菌根合成》 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN117652343A (en) * | 2024-01-31 | 2024-03-08 | 云南上智科技有限公司 | Mycorrhiza synthesis method of target plant |
| CN117652343B (en) * | 2024-01-31 | 2024-04-16 | 云南上智科技有限公司 | Mycorrhiza synthesis method of target plant |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN104170656B (en) | Grafting method for thin-skin muskmelons | |
| CN108029431A (en) | Citrus grafting seedlings-growing method | |
| CN105165331A (en) | Cucumber planting method | |
| CN105325147A (en) | Method for cultivating and planting lycium ruthenicum murray | |
| CN104429520A (en) | Vegetable planting method | |
| CN115250812A (en) | Germination accelerating method and breeding and seedling method for blueberry seeds | |
| CN103688718A (en) | Culturing method of aralia elata seem | |
| CN104025874A (en) | Organic planting method of celery | |
| CN106576721A (en) | High-yield eggplant planting method | |
| KR20130051649A (en) | Method of cultivating vegetable fruit using rye | |
| CN106717829A (en) | The implantation methods of Panax Japonicus Var. Major | |
| CN107691105A (en) | The breeding method and its cultural method of a kind of hickory chick | |
| CN107821074A (en) | A kind of implantation methods for shortening leek breeding time | |
| CN107223521A (en) | A kind of method that poplar forest winter interplants leek | |
| CN107306635A (en) | A kind of implantation methods for improving rose yield and quality | |
| CN106718485A (en) | A kind of seedling-raising technique of fructus crataegi pinnatifidae | |
| CN109496724A (en) | A kind of efficient implantation methods of tassel flower | |
| CN108551962A (en) | A kind of method of Ling Yaoxing and peach interplanting | |
| CN107211700A (en) | A kind of nuisanceless watermelon breeding method | |
| CN111826291A (en) | Biological bacterium solution for improving survival rate of trees in arid and saline-alkali areas as well as preparation and application thereof | |
| CN108029449A (en) | A kind of method of Yunnan Oak Tree seed seedling-raising inoculation India truffle | |
| CN110612868A (en) | Organic strawberry planting method | |
| CN106305115A (en) | Amaranthus tricolor planting method | |
| CN102177779A (en) | Method for purifying wild ginseng seeds | |
| CN107996278A (en) | A kind of method of hazel tree seed seedling-raising inoculation India truffle |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20180508 |