CN107941681B - 鉴定生物样品中定量细胞组成的方法 - Google Patents

鉴定生物样品中定量细胞组成的方法 Download PDF

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CN107941681B
CN107941681B CN201711262337.XA CN201711262337A CN107941681B CN 107941681 B CN107941681 B CN 107941681B CN 201711262337 A CN201711262337 A CN 201711262337A CN 107941681 B CN107941681 B CN 107941681B
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CN107941681A (zh
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斯文·欧莱克
乌利齐·霍夫缪勒
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    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/10Investigating individual particles
    • G01N15/14Optical investigation techniques, e.g. flow cytometry
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    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6881Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for tissue or cell typing, e.g. human leukocyte antigen [HLA] probes
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    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
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    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
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    • C12Q2600/00Oligonucleotides characterized by their use
    • C12Q2600/154Methylation markers
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/10Investigating individual particles
    • G01N2015/1006Investigating individual particles for cytology

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CN201711262337.XA 2013-04-19 2014-04-22 鉴定生物样品中定量细胞组成的方法 Active CN107941681B (zh)

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US201361813802P 2013-04-19 2013-04-19
US61/813,802 2013-04-19
EP13173442.8 2013-06-24
EP13173442 2013-06-24
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Families Citing this family (29)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP3029150B1 (en) * 2014-12-05 2018-08-15 Epiontis GmbH Epigenetic method for the identification of follicular T-helper-(TFH-) cells
GB201516975D0 (en) * 2015-09-25 2015-11-11 Epiontis Gmbh PARK2 as epigenetic marker for the identification of immune cells, in particular monocytes
GB201516972D0 (en) * 2015-09-25 2015-11-11 Epiontis Gmbh LRP5 as epigenetic marker for the identification of immune cells, in particular B-cells
JP6100867B1 (ja) * 2015-11-06 2017-03-22 振武 曽 免疫状態の分析のための検査方法
CN106520693B (zh) * 2016-10-20 2019-11-22 清华大学 记忆b细胞前体细胞及其用途
EP3360971A1 (en) * 2017-02-09 2018-08-15 Epiontis GmbH Improved method for epigenetic immune cell counting
EP3382033B1 (en) * 2017-03-30 2020-08-05 Rheinisch-Westfälische Technische Hochschule (RWTH) Aachen Method for determining blood counts based on dna methylation
DE102017004108A1 (de) * 2017-05-01 2018-11-08 Rheinisch-Westfälische Technische Hochschule (Rwth) Aachen Verfahren zur Bestimmung der Zellzahl von eukaryotischen Zellen
DE102017125013B4 (de) * 2017-10-25 2019-10-17 Epiontis Gmbh MCC als epigenetischer Marker zur Identifizierung von Immunzellen, insbesondere basophiler Granulozyten
DE102017125019B4 (de) * 2017-10-25 2019-10-17 Epiontis Gmbh PDCD1 als epigenetischer Marker zur Identifizierung von Immunzellen, insbesondere PD1+ Zellen
DE102017125150B4 (de) * 2017-10-26 2019-10-10 Epiontis Gmbh Endosialin (CD248) als epigenetischer Marker zur Identifizierung von Immunzellen, insbesondere naïver CD8+ T-Zellen
DE102017125335B4 (de) * 2017-10-27 2019-10-17 Epiontis Gmbh Amplikon-Region als epigenetischer Marker zur Identifizierung von Immunzellen, insbesondere nicht-klassischer Monozyten
US20200299677A1 (en) * 2017-10-27 2020-09-24 Juno Diagnostics, Inc. Devices, systems and methods for ultra-low volume liquid biopsy
DE102017126248B4 (de) * 2017-11-09 2019-10-17 Epiontis Gmbh ERGIC1 als epigenetischer Marker zur Identifizierung von Immunzellen, insbesonderemonozytischem myeloid-abgeleiteten Suppressor-Zellen (mMDSCs)
CN109897822B (zh) * 2017-12-11 2023-04-04 复旦大学 一组人永生化b淋巴细胞系的建立和应用
WO2020007951A1 (en) * 2018-07-05 2020-01-09 Epiontis Gmbh Epigenetic method to detect and distinguish ipex and ipex-like syndromes, in particular in newborns
DE102018116353B4 (de) 2018-07-05 2020-06-10 Epiontis Gmbh Verfahren zum Nachweis und zur Zählung epigenetischer Immunzellen in humanen Blutproben für Immundiagnostik und Neugeborenen-Screening
CN109439626B (zh) * 2018-11-09 2022-10-14 复旦大学附属中山医院 一种有助于体外获得Th22细胞的组合物及其用途
CN109576350B (zh) * 2019-01-18 2021-01-29 深圳恒特基因有限公司 一种dna与rna同时定量的试剂盒、方法及质控方法
CN109988844B (zh) * 2019-04-30 2022-10-04 上海奕谱生物科技有限公司 基于甲基化修饰的肿瘤标记物stamp-ep7及其应用
DE102020108560B4 (de) 2020-03-27 2022-03-03 Precision For Medicine Gmbh CBX6 als epigenetischer Marker für die Identifizierung von Immunzellen, insbesondere Gedächtnis-B-Zellen
JPWO2022191293A1 (enExample) * 2021-03-12 2022-09-15
CN113667673B (zh) * 2021-07-15 2024-02-06 武汉血液中心 一种类孟买血型FUT1 236delG等位基因及其检测方法和应用
US20250003007A1 (en) * 2021-10-04 2025-01-02 H. Lee Moffitt Cancer Center And Research Institute Inc. Dna methylation signatures for predicting response to immunotherapy
KR102559496B1 (ko) * 2022-04-26 2023-07-25 연세대학교 산학협력단 핵산의 메틸화 여부 확인용 조성물 및 핵산의 메틸화 여부를 확인하는 방법
WO2024243375A1 (en) * 2023-05-23 2024-11-28 Yale University Compositions and methods of determining lupus nephritis class
DE102023120207A1 (de) 2023-07-28 2025-01-30 Rheinisch-Westfälische Technische Hochschule Aachen, Körperschaft des öffentlichen Rechts Verbessertes Verfahren zur Quantifizierung der zellulären Zusammensetzung einer biologischen Probe
CN118067591B (zh) * 2024-04-17 2024-07-12 南京佰抗生物科技有限公司 一种用于血液分析的耗材
CN119372296B (zh) * 2024-12-24 2025-06-13 北京致谱医学检验实验室有限公司 基于基因甲基化检测实现人b淋巴细胞计数的试剂盒

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1330513A (zh) * 1999-04-06 2002-01-09 东卡罗来纳大学 低腺苷反义寡核苷酸、组合物、试剂盒及与支气管紧缩、肺炎、过敏及表面活性剂缺失相关的通气途径疾病的治疗方法
EP2141245A1 (en) * 2008-07-03 2010-01-06 Ivana Türbachova DNA methylation analysis of regulatory T cells through DNA-methylation analysis of the TSDR region of the gene foxP3
EP2248913A1 (en) * 2009-04-28 2010-11-10 Epiontis GmbH Epigenetic marker for the identification of natural killer cells
WO2012012693A2 (en) * 2010-07-23 2012-01-26 President And Fellows Of Harvard College Methods for detecting signatures of disease or conditions in bodily fluids
WO2013014122A1 (en) * 2011-07-22 2013-01-31 Epiontis Gmbh Epigenetic marker for the identification of natural killer cells

Family Cites Families (15)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5773290A (en) * 1996-10-07 1998-06-30 Wisconsin Alumni Research Foundation Mammary gland-specific promoters
JP3783808B2 (ja) * 1997-05-19 2006-06-07 シスメックス株式会社 白血球分類計数用試薬
JP4012339B2 (ja) * 1999-05-24 2007-11-21 株式会社堀場製作所 血球分類マトリックス図表示装置、血球分類マトリックス図の表示方法および血球分類マトリックス図表示プログラムを記録した記録媒体
WO2004050706A2 (en) 2002-12-03 2004-06-17 Medical Research Council Regulatory t-cells
DE102004016437A1 (de) * 2004-04-04 2005-10-20 Oligene Gmbh Verfahren zur Erkennung von Signaturen in komplexen Genexpressionsprofilen
JP2005333987A (ja) * 2004-05-06 2005-12-08 Veridex Llc 悪性血液疾患の予後
US7851154B2 (en) * 2005-07-22 2010-12-14 Simon Daniel Spivack GC tag-modified bisulfite genomic DNA sequencing for continuous methylation spectra
EP2097538A4 (en) * 2006-12-07 2011-11-30 Switchgear Genomics TRANSCRIPTION REAGULATION ELEMENTS OF BIOLOGICAL PATHS, TOOLS AND METHODS
EP2199411B1 (en) 2008-12-16 2015-05-06 Epiontis GmbH Epigenetic markers for the identification of CD3 positive T-lymphocytes
US20120028816A1 (en) * 2009-03-31 2012-02-02 Warren Stephen T Methods and systems for screening for and diagnosing dna methylation associated with autism spectrum disorders
EP2440927A4 (en) * 2009-06-09 2012-12-05 Banner Sun Health Res Inst METHOD AND SYSTEM FOR DETECTING, DIAGNOSING AND MONITORING THE PROGRESSION OF ALZHEIMER'S DISEASE
EP2354242A1 (en) * 2010-02-03 2011-08-10 Epiontis GmbH Assay for determining the type and/or status of a cell based on the epigenetic pattern and the chromatin structure
ES2657846T3 (es) 2010-04-29 2018-03-07 Epiontis Gmbh Detección de células inmunes, en particular linfocitos T mediante análisis de metilación de ADN de los genes CCR6
JP5583629B2 (ja) * 2011-04-28 2014-09-03 シスメックス株式会社 白血球の分類計数方法、白血球分類試薬キット及び白血球分類試薬
EP2518160A1 (en) * 2011-04-29 2012-10-31 Universitätsklinikum Freiburg Mitochondrial nucleic acid as a marker for autoimmune and autoinflammatory diseases

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1330513A (zh) * 1999-04-06 2002-01-09 东卡罗来纳大学 低腺苷反义寡核苷酸、组合物、试剂盒及与支气管紧缩、肺炎、过敏及表面活性剂缺失相关的通气途径疾病的治疗方法
EP2141245A1 (en) * 2008-07-03 2010-01-06 Ivana Türbachova DNA methylation analysis of regulatory T cells through DNA-methylation analysis of the TSDR region of the gene foxP3
EP2248913A1 (en) * 2009-04-28 2010-11-10 Epiontis GmbH Epigenetic marker for the identification of natural killer cells
WO2012012693A2 (en) * 2010-07-23 2012-01-26 President And Fellows Of Harvard College Methods for detecting signatures of disease or conditions in bodily fluids
WO2013014122A1 (en) * 2011-07-22 2013-01-31 Epiontis Gmbh Epigenetic marker for the identification of natural killer cells

Non-Patent Citations (5)

* Cited by examiner, † Cited by third party
Title
DNA demethylation in the human FOXP3 locus discriminates regulatory T cells from activated FOXP3+ conventional T cells;Udo Baron等;《Eur. J. Immunol. 》;20070901;第37卷;2378-2389 *
DNA Methylation Analysis as a Tool for Cell Typing;Udo Baron等;《Epigenetics》;20060310;55-60 *
Epigenetic quantifiation of tumor-infitrating T-lymphocytes;Jalid sehouli等;《Epigenetics》;20110228;第6卷(第2期);236-246 *
Quantitative DNA Methylation Analysis of FOXP3 as a New Method for Counting Regulatory T Cells in Peripheral Blood and Solid Tissue;Georg Wieczorek等;《Cancer Res》;20090115;第69卷(第2期);599-608 *
原发性干燥综合征患者外周血Th17细胞的检测及意义;王文军 等;《中国实验诊断学》;20110630(第6期);1039-1040 *

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CA2904658C (en) 2023-10-17
US10894984B2 (en) 2021-01-19
CN105189783A (zh) 2015-12-23
US20180291447A1 (en) 2018-10-11
CN105189783B (zh) 2020-08-07
EP3327141B1 (en) 2020-01-29
EP3561074A1 (en) 2019-10-30
PL3327141T3 (pl) 2020-07-27
DK3327141T3 (da) 2020-05-04
JP2016515397A (ja) 2016-05-30
JP6577005B2 (ja) 2019-09-18
EP3561074B1 (en) 2024-03-20
US20160024578A1 (en) 2016-01-28
JP6590790B2 (ja) 2019-10-16
PL2986735T3 (pl) 2019-08-30
JP2018064570A (ja) 2018-04-26
WO2014170497A2 (en) 2014-10-23
CN107941681A (zh) 2018-04-20
EP2986735B1 (en) 2019-03-06
EP3327141A1 (en) 2018-05-30
US10208346B2 (en) 2019-02-19
WO2014170497A3 (en) 2014-12-11
EP2986735A2 (en) 2016-02-24
CA2904658A1 (en) 2014-10-23

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