CN107929127A - A kind of preparation method of radix pseudostellariae extracting solution and its application in anti-wrinkle cosmetic - Google Patents
A kind of preparation method of radix pseudostellariae extracting solution and its application in anti-wrinkle cosmetic Download PDFInfo
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- A61K8/00—Cosmetics or similar toiletry preparations
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- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
- A61K8/97—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
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- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/33—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds containing oxygen
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- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/40—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds containing nitrogen
- A61K8/44—Aminocarboxylic acids or derivatives thereof, e.g. aminocarboxylic acids containing sulfur; Salts; Esters or N-acylated derivatives thereof
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- A61Q19/08—Anti-ageing preparations
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- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
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Abstract
The present invention provides a kind of preparation method of radix pseudostellariae extracting solution and its application in anti-wrinkle cosmetic.Using green non-poisonous Novel extraction method, functional component is obtained（Polysaccharide, amino acid, cyclic peptide, aliphatic acid etc.）The high radix pseudostellariae extracting solution of content, cosmetics are prepared as raw material, to being irradiated because of UVB caused by cellular damage have prevention and repair, there is skin wrinkle resisting.
Description
Technical field
A kind of preparation method the present invention relates to radix pseudostellariae extracting solution and its application in anti-wrinkle cosmetic.
Background technology
Wrinkle is skin aging as a result, being that skin lacks moisture, surface fat is reduced, the result of flexibility decrease.Wrinkle
The appearance of face is directly affected, shows the aging of a people.
Wrinkle of skin, be because skin epidermis it is inhomogenous collapse caused by.Skin is made of three-decker, including table
Cortex, skin corium, subcutaneous fat；Skin corium contains collagen, elastin and other fibers and constitutes support skin
Skeleton.And the skin that makes of these materials seems smooth youth, these same materials be also vulnerable to ultraviolet light and ozone or its
The damage of his oxidative factors.Wherein UVA long wave ultraviolets wavelength 380-420nm, can be as deep as corium and subcutaneous tissue aspect；And
UVB short wave ultraviolet wavelength 200-280nm, up to epidermis, ozone can only generally pass through cuticula.
Increase with the age of people, the differentiation of Skin Cell can be slower and slower, and corium is gradually thinning, and subepidermal fat is thin
Born of the same parents start atrophy, and skin histology becomes more and more loose.Until skin follows the string, just no longer restored after being extruded, from
And form skin sagging, produce wrinkle.
The continuous repeating motion of centrifugal force and musculus cutaneus affects two kinds of fibers：Collagenous fibres and elastomer.The former ties up
The toughness of skin is hold, the latter then ensures the softness of skin.There is one layer of invisible corium below epidermis, it
It is important with as epidermis, it is basis and the nutrient source of epidermis.
In skin corium, collagenous fibres and elastomer are constantly be generated, and are constantly withered away.In general, enzyme can incite somebody to action
The fiber destruction of those most agings is fallen, and at the same time, fibroblast can produce new fiber to replace them again.But with
The passage of time, after this metabolism occurs that problem, people are getting in years, corium mother cell is to the destructive power of antienzyme
The quality and quantity of reduced capability, collagenous fibres and elastomer also declines therewith, and the interface of epidermis and corium is also begun to
Decline is loose.In addition, epidermis and corium can increasingly tend to it is disconnected from each other, sometimes even can fully nonlinear water wave.Skin is just opened
There is wrinkle at leisure in beginning.Therefore the cosmetics of effect brilliance are developed, fundamentally changes the quality of skin, has prevention to wrinkle
Effect, play the role of delaying facial aging, have a vast market space.
Radix pseudostellariae, is pinkwort caryophyllaceous ginseng Pseudostellaria heterophylla（Miq.）Pax ex
The dried root of Pax et Hoffm..Sweet in flavor, slight bitter, it is mild-natured；Returns spleen, lung channel；Replenish qi to invigorate the spleen, it is promoting production of body fluid and nourishing the lung.Radix pseudostellariae is
Fujian Province's genunie medicinal materials, originate in Zherong County.It is one of Fujian production Chinese medicine trade brand " nine taste of good fortune " kind.
Has reported in literature, it is a variety of to contain amino acid, polysaccharide volatile ingredient, trace element and cyclic peptide etc. in radix pseudostellariae
The active ingredient of type.Wherein warmed up using smart ammonia, the content of glutamic acid as highest, it is amino acid contained to be just beneficial to skin-nourishing
Kind, this is rare in general plant；In addition iron, manganese, zinc, copper, molybdenum, cobalt, the selenium and other trace elements contained in radix pseudostellariae have
Application of the machine thing in cosmetics is also critically important；In addition, saponin(e and hexadecylic acid, linoleic acid, single glyceride in radix pseudostellariae
Deng content it is not low, these be all be commonly used in cosmetics improve cuticula additive, they be attained by moisturizing, profit
Skin, compact, anti-ageing effect.
Plant cyclic peptide is rich in radix pseudostellariae, it is separated to obtain heterophyllin A-H（I-VIII）,
pseudostellaria A-H（IX-XVI）16 kinds of cyclic peptide compounds.The research of this team finds that radix pseudostellariae small molecule cyclic peptide has
Have a cell-penetrating effect, reduce skin collagen and be lost in, significantly reduce skin microgroove, it is more aobvious compact it is moist.
Glucan and Pectic polysaccharides are rich in radix pseudostellariae, are pure natural, gentle, nonirritant water-soluble polymers,
They can increase the stability of beauty product emulsification system, keep its moisture in being formulated, and can reduce the saturating of moisture of skin
Skin lesion is lost.The high-hydroscopicity of polysaccharide and good film forming perfect adaptation, reach good moisturizing moisture-keeping function.
The existing extracting method for radix pseudostellariae, functional component in obtained Radix Pseudostellariae extract（Glucose, amino
Sour, total cyclic peptide, aliphatic acid etc.）Content is low, and anti-wrinkle effect is undesirable, and therefore, there is an urgent need for develop a kind of effective component extraction rate
The preparation method of radix pseudostellariae extracting solution high, anti-wrinkle effect is good.
The content of the invention
It is a primary object of the present invention to provide a kind of preparation method and applications of radix pseudostellariae extracting solution, using green nothing
Malicious Novel extraction method, obtains functional component（Polysaccharide, amino acid, cyclic peptide, aliphatic acid etc.）The high radix pseudostellariae extracting solution of content, will
It prepares cosmetics as raw material, to being irradiated because of UVB caused by cellular damage have prevention and repair, resist with skin
Wrinkle function.
To achieve these goals, technical scheme is as follows：
A kind of preparation method of radix pseudostellariae extracting solution, comprises the following specific steps that：
1）The preparation of radix pseudostellariae water-soluble substances：
Dry radix pseudostellariae pulverizing medicinal materials are weighed to 20 mesh, after adding 5-10 times of weight water soaked overnights, 100 DEG C of refluxing extractions
4h；Filtering, collects filtrate；Contain the water-soluble substanceses such as Pseudostellaria Polysaccharide and amino acid in filtrate.
2）The preparation of radix pseudostellariae apolar substance：
Usual radix pseudostellariae apolar substance extraction need to use the organic solvents such as ethyl acetate, n-butanol；In view of radix pseudostellariae is nonpolar
Material such as cyclic peptide, aliphatic acid etc. are good skin-care effect, but Extraction solvent cannot function as cosmetic material, and the present invention uses
Nontoxic green eutectic solvent extraction radix pseudostellariae apolar substance.
Using eutectic solvent glycine betaine-glycerine mixed solution as Extraction solvent, radix pseudostellariae water is carried the rear dregs of a decoction into
Row apolar substance ultrasound assisted extraction.Ultrasonic wave added eutectic solvent extraction technological parameter be：Add dregs of a decoction weight 5-10
Glycine betaine again is 1 with glycerine molar ratio:The mixed solution of 1-3, water content 20-40wt%, 40-60 DEG C of ultrasonic extraction temperature
, power 500W, 15 min of time.
3）Radix pseudostellariae water-soluble substances and radix pseudostellariae apolar substance compounding：
Merge radix pseudostellariae water-soluble extractive and the nonpolar extract of radix pseudostellariae, be in being concentrated under reduced pressure into concentration on Rotary Evaporators
Per 1mL equivalent to radix pseudostellariae crude drug amount 0.5-5g, the radix pseudostellariae extracting solution is obtained.
Using：Application of the radix pseudostellariae extracting solution in anti-wrinkle cosmetic.
The remarkable advantage of the present invention：
（1）Using eutectic solvent glycine betaine-glycerine mixed solution as Extraction solvent, the rear dregs of a decoction are carried to radix pseudostellariae water and are carried out
Apolar substance ultrasound assisted extraction, glycine betaine-glycerine mixed solution contribute to the dissolution of cyclic peptide and aliphatic acid in radix pseudostellariae,
Assisting ultrasonic is extracted again, significantly improves the recovery rate of cyclic peptide and aliphatic acid.Meanwhile solvent glycine betaine, glycerine need not remove, two
Person is allowed for cosmetic material in itself.Glycine betaine can remove old useless cutin, and can effectively catch moisture, lock skin, reach
Long-acting moistening effect；Glycerine is that moisturizer is commonly used in cosmetics；Avoid in the prior art using ethyl acetate, n-butanol etc.
Organic solvent goes solvent process as the later stage necessary to the Extraction solvent of radix pseudostellariae apolar substance.
（2）Radix pseudostellariae extracting solution causes UVB irradiations fibroblast damage with prevention and repair.
Brief description of the drawings
Fig. 1 is the HPLC collection of illustrative plates of radix pseudostellariae extracting solution.
Embodiment
In order to further illustrate the technological means and its effect of the invention taken, below in conjunction with the preferred reality of the present invention
Example is applied to be described in detail.
Embodiment 1
A kind of preparation method of radix pseudostellariae extracting solution, comprises the following specific steps that：
1）The preparation of radix pseudostellariae water-soluble substances：
Dry radix pseudostellariae pulverizing medicinal materials are weighed to 20 mesh, after adding 5 times of weight water soaked overnights, 100 DEG C of refluxing extraction 4h, mistake
Filter, obtains filtrate A；
2）The preparation of radix pseudostellariae apolar substance：
Using eutectic solvent glycine betaine-glycerine mixed solution as Extraction solvent, added in the dregs of a decoction after radix pseudostellariae water carries
The glycine betaine of 5 times of dregs of a decoction weight-glycerine mixed solution, carries out apolar substance ultrasound assisted extraction, wherein, ultrasonic extraction temperature
40 DEG C, power 500W, 15 min of time of degree, after extraction, filtering, obtains liquor B；
In the glycine betaine-glycerine mixed solution, glycine betaine is 1 with glycerine molar ratio:1st, water content 20wt%.
3）Radix pseudostellariae water-soluble substances and radix pseudostellariae apolar substance compounding：
Merging filtrate A and liquor B, in be concentrated under reduced pressure on Rotary Evaporators concentration be per 1mL equivalent to radix pseudostellariae crude drug amount 5g,
Obtain the radix pseudostellariae extracting solution.
Embodiment 2
A kind of preparation method of radix pseudostellariae extracting solution, comprises the following specific steps that：
1）The preparation of radix pseudostellariae water-soluble substances：
Dry radix pseudostellariae pulverizing medicinal materials are weighed to 20 mesh, after adding 6 times of weight water soaked overnights, 100 DEG C of refluxing extraction 4h, mistake
Filter, obtains filtrate A；
2）The preparation of radix pseudostellariae apolar substance：
Using eutectic solvent glycine betaine-glycerine mixed solution as Extraction solvent, added in the dregs of a decoction after radix pseudostellariae water carries
The glycine betaine of 6 times of dregs of a decoction weight-glycerine mixed solution, carries out apolar substance ultrasound assisted extraction, wherein, ultrasonic extraction temperature
50 DEG C, power 500W, 15 min of time of degree, after extraction, filtering, obtains liquor B；
The glycine betaine is 1 with glycerine molar ratio:3rd, water content 30wt%.
3）Radix pseudostellariae water-soluble substances and radix pseudostellariae apolar substance compounding：
Merging filtrate A and liquor B, are equivalent to radix pseudostellariae crude drug amount per 1mL in being concentrated under reduced pressure into concentration on Rotary Evaporators
1.0g, obtains the radix pseudostellariae extracting solution.
Embodiment 3
A kind of preparation method of radix pseudostellariae extracting solution, comprises the following specific steps that：
1）The preparation of radix pseudostellariae water-soluble substances：
Dry radix pseudostellariae pulverizing medicinal materials are weighed to 20 mesh, after adding 10 times of weight water soaked overnights, 100 DEG C of refluxing extraction 4h,
Filtering, obtains filtrate A；
2）The preparation of radix pseudostellariae apolar substance：
Using eutectic solvent glycine betaine-glycerine mixed solution as Extraction solvent, added in the dregs of a decoction after radix pseudostellariae water carries
The glycine betaine of 10 times of dregs of a decoction weight-glycerine mixed solution, carries out apolar substance ultrasound assisted extraction, wherein, ultrasonic extraction
Temperature 60 C, power 500W, 15 min of time, after extraction, filtering, obtains liquor B；
The glycine betaine is 1 with glycerine molar ratio:2nd, water content 40wt%.
3）Radix pseudostellariae water-soluble substances and radix pseudostellariae apolar substance compounding：
Merging filtrate A and liquor B, are equivalent to radix pseudostellariae crude drug amount per 1mL in being concentrated under reduced pressure into concentration on Rotary Evaporators
0.5g, obtains the radix pseudostellariae extracting solution.
Comparative example 1
Step 2）In glycine betaine-glycerine mixed solution substituted with ethyl acetate, remaining operating process is the same as embodiment 1.
Comparative example 2
Step 2）In glycine betaine-glycerine mixed solution substituted with n-butanol, remaining operating process is the same as embodiment 1.
Radix pseudostellariae extracting solution detects：
1）Radix pseudostellariae extracts polysaccharide determination
Precision draws 5.0ml radix pseudostellariae extracting solution samples in 50ml centrifuge tubes, adds appropriate absolute ethyl alcohol, mixes, makes in sample
Final concentration of the 90% of ethanol, 4 DEG C of standing 1h, 10min, abandoning supernatant are centrifuged in 4000r/min.It is heavy so to collect several times
Form sediment.Sediment is washed to colourless with ethanol in proper amount.10min, abandoning supernatant are centrifuged with 4000r/min, precipitation heating removes second
Alcohol, is dissolved with water, and constant volume shakes up in 100ml volumetric flasks, as test solution.
The DEXTROSE ANHYDROUS reference substance 10mg of dry constant weight is accurately weighed, is dissolved in water, constant volume to 10ml, obtains 1.0mg/ml
Glucose control product stock solution.Precision draws reference substance stock solution 0.2,0.3,0.4,0.5,0.6,0.8ml, puts 10ml tool plugs
In test tube, add water to make to volume to be 2.0ml into each pipe respectively, then add 5% phenol solution 1.0ml to each test tube successively, shake up,
Concentrated sulfuric acid 5.0ml is rapidly added successively, is shaken up；Using water as blank, absorbance is measured at spectrophotometer 490nm wavelength,
With concentration of glucose (mg/m1) for abscissa, absorbance draws standard curve for ordinate.
Precision draws test solution 2.0ml, is placed in tool plug test tube, measures absorbance, solution concentration is calculated as follows.
In formula -- total starches content is in terms of DEXTROSE ANHYDROUS in sample, g/100ml；C-- must be for examination by regression equation calculation
Solution concentration, mg/ml；V-- test solution constant volumes, ml；M-- sample volumes, ml.
2）Radix Pseudostellariae extract amino acid, total cyclic peptide, total fatty acids liquid chromatographic detection
Precision pipettes the radix pseudostellariae extracting solution of certain volume, ethyl acetate extraction, and extract on Rotary Evaporators in being recovered under reduced pressure
Ethyl acetate is done near, and the spectrum methanol dissolving of residue additive color, moves in volumetric flask, add methanol to be filtered to scale with 0.45 μm of micropore
Membrane filtration.Test solution concentration：Per 1mL equivalent to radix pseudostellariae crude drug amount 1g.
ODS C18 chromatographic columns, with acetonitrile-phosphoric acid mobile phase, gradient elution, Detection wavelength 203nm, flow velocity 1mL/min,
30 DEG C of column temperature, 20 μ L of sample size.Liquid chromatogram（HPLC）Figure is shown in Fig. 1.
Using area normalization method, by following formula：
3）The content of active ingredient in radix pseudostellariae extracting solution
Influence of the different extraction processes of table 1 to active constituent content in radix pseudostellariae extracting solution
Usual radix pseudostellariae apolar substance extraction need to use the organic solvents such as ethyl acetate, n-butanol, but it is former to belong to cosmetics disabling
Material, later stage need to carry out solvent operation.The present invention is molten as extracting using eutectic solvent glycine betaine-glycerine mixed solution
Agent, then assisting ultrasonic extraction, have excellent extraction effect to cyclic peptide, aliphatic acid radix pseudostellariae apolar substance.Though the extraction of cyclic peptide
Rate is slightly below ethyl acetate and n-butanol, but there was no significant difference；Ethyl acetate and n-butanol are higher than to the recovery rate of aliphatic acid
Deng organic solvent.
Especially the non-pole such as Radix pseudostellariae cyclic peptides, aliphatic acid can be made in the case where avoiding organic solvent use in the present invention
The property effective dissolution of material；Eutectic solvent used is made of glycine betaine-glycerine, and glycine betaine can remove old useless cutin, and can have
Effect catches moisture, locks skin, reaches long-acting moistening effect；Glycerine is that moisturizer is commonly used in cosmetics.The two allows to use
In cosmetic material, asepsis environment-protecting, solvent can reach the effect to play the same tune on different musical instruments without removing.
Detection of the radix pseudostellariae extracting solution in terms of skin wrinkle resisting effect
A kind of radix pseudostellariae extracting solution of this experiment discussion is to ultraviolet B radiation（UVB）Irradiation causes Cultured Mouse fibroblast
（L929）The prevention and repair of damage.
Cell culture and inoculation
L cell（L929）, with the sugared nutrient solutions of the DMEM high containing 10% hyclone, at 37 DEG C, 5% CO2Ring
Cultivated in border, growth period cell of taking the logarithm, with trypsase-EDTA solution digestions, adjustment cell concentration is 5 × 105A/
ML, 96 orifice plates, 24 h of cellar culture are seeded to according to 100 μ L/hole.The total cyclic peptide of radix pseudostellariae is dissolved in without hyclone DMEM
Basic culture solution, the cell in 96 orifice plates is grouped, every group sets 6 multiple holes.
Experiment packet and administration
Growth selection exponential phase of growth cell in good condition, is randomly divided into UVB irradiation groups after sample pretreatment, after UVB irradiations
Sample treatment group.Desk-top ultraviolet irradiation instrument is applied in UVB irradiations, is opposite to cell and is irradiated, energy density is 300 mJ/cm2,
Height 10cm, causes fibroblast to damage.
UVB irradiation groups after extracting solution pretreatment, are irradiated after 24 h of sample incubation cell with UVB, liquid culture 24 are changed after irradiation
h；Sample treatment group after UVB irradiations, 24 h of extracting solution incubated cell after UVB irradiations.Extracting solution intervenes cell, adds sample 100
μ L, it is respectively 0.125,0.25,0.5,1.0,2.0,3.0 mg/mL to make its final concentration, while sets blank control group, every group 5
Multiple holes, MTT methods detection comparative survival rate of cells after 24 h.
Method detects cell relative survival
After sample-adding culture 48h, 5 mg/20 μ L of mL MTT solution are added per hole into Tissue Culture Plate.Put in incubator and keep away
Light is incubated 4 h.Supernatant is abandoned in suction, and 100 μ L of DMSO are added per hole.Lucifuge, puts 20 min of shaking table low-speed oscillation.
Absorbance value A is measured at 570 nm wavelength with microplate reader.
Relative survival=（An -A0）/（Ax-A0）× 100%
An:Experimental group absorbance；
Ax:Negative control group absorbance；
A0:Blank control group absorbance.
Statistical analysis
Using 15.0 softwares of SPSS, data are with average（）Form represent, the comparison inspection between two groups, two with
Comparison between upper group is handled with ANOVA/Holm-Sidak statistical softwares.It is that difference is statistically significant with P ＜ 0.05, P ＜
0.01 is to have highly significant difference.
Experimental result
Radix pseudostellariae extract concentration>It can inhibit fibroblast proliferation, 0.1mg/mL during 3.0 mg/mL<Radix pseudostellariae extracting solution is dense
Degree<It can promote fibroblast proliferation during 2.0 mg/mL, the best use of concentration is 1.7 mg/mL；Cytoactive after UVB irradiations
Decline 36.83%；Compared with simple UVB irradiates, fiber can be made into by being handled after UVB pre-irradiations or irradiation with radix pseudostellariae extracting solution
Cell survival rate improves.Low concentration radix pseudostellariae extracting solution can promote fibroblast proliferation, so that caused by being irradiated because of UVB
Cellular damage has prevention and repair.Experimental data is shown in Table 2.
2 radix pseudostellariae extracting solution of table causes UVB irradiations the prevention and repair of L929 cellular damages（Survival rate %, n=5）
Note：Model group 1 compared with Normal group,#P≤0.05,## P≤0.01
Model group 2 is compared with Normal group, * P≤0.05, * * P≤0.01
Administration group compared with negative control group 1,▲P≤0.05,▲▲ P≤0.01
Administration group compared with negative control group 2,▼P≤0.05,▼▼ P≤0.01
The foregoing is merely presently preferred embodiments of the present invention, all equivalent changes done according to scope of the present invention patent are with repairing
Decorations, should all belong to the covering scope of the present invention.
Claims (3)
1. a kind of preparation method of radix pseudostellariae extracting solution, it is characterised in that comprise the following specific steps that：
1）The preparation of radix pseudostellariae water-soluble substances：
Dry radix pseudostellariae pulverizing medicinal materials are weighed to 20 mesh, after adding 5-10 times of weight water soaked overnights, 100 DEG C of refluxing extractions
4h, filtering, obtains filtrate A；
2）The preparation of radix pseudostellariae apolar substance：
Using eutectic solvent glycine betaine-glycerine mixed solution as Extraction solvent, added in the dregs of a decoction after radix pseudostellariae water carries
The glycine betaine of 5-10 times of dregs of a decoction weight-glycerine mixed solution, carries out apolar substance ultrasound assisted extraction, wherein, ultrasound carries
40-60 DEG C of temperature is taken, power 500W, 15 min of time, after extraction, filtering, obtains liquor B；
3）Radix pseudostellariae water-soluble substances and radix pseudostellariae apolar substance compounding：
Merging filtrate A and liquor B, are equivalent to radix pseudostellariae crude drug amount per 1mL in being concentrated under reduced pressure into concentration on Rotary Evaporators
0.5-5g, obtains the radix pseudostellariae extracting solution.
A kind of 2. preparation method of radix pseudostellariae extracting solution according to claim 1, it is characterised in that the glycine betaine-the third
In three mixed alkoxide solutions, glycine betaine is 1 with glycerine molar ratio:1-3, water content 20-40wt%.
3. the application of radix pseudostellariae extracting solution made from preparation method as claimed in claim 1 or 2, it is characterised in that described
Application of the radix pseudostellariae extracting solution in anti-wrinkle cosmetic.
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| CN109358153A (en) * | 2018-12-27 | 2019-02-19 | 福建省中医药研究院(福建省青草药开发服务中心） | The thin-layer identification method of liposoluble constituent in a kind of radix pseudostellariae medicinal material |
| CN109369821A (en) * | 2018-11-28 | 2019-02-22 | 四川恒通动保生物科技有限公司 | A kind of high efficiency extraction preparation method of astragalus polyose |
| CN110075154A (en) * | 2019-06-04 | 2019-08-02 | 南京林业大学 | A method of saponin(e active component is extracted from black wattle bark based on depth congruent melting solvent |
| CN113181683A (en) * | 2021-05-12 | 2021-07-30 | 山东宏济堂制药集团股份有限公司 | Radix pseudostellariae standard control substance and separation and purification method and detection method thereof |
| CN116332894A (en) * | 2023-03-22 | 2023-06-27 | 广东丸美生物技术股份有限公司 | Method for preparing cocoa extract and cosmetic |
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Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109369821A (en) * | 2018-11-28 | 2019-02-22 | 四川恒通动保生物科技有限公司 | A kind of high efficiency extraction preparation method of astragalus polyose |
| CN109358153A (en) * | 2018-12-27 | 2019-02-19 | 福建省中医药研究院(福建省青草药开发服务中心） | The thin-layer identification method of liposoluble constituent in a kind of radix pseudostellariae medicinal material |
| CN110075154A (en) * | 2019-06-04 | 2019-08-02 | 南京林业大学 | A method of saponin(e active component is extracted from black wattle bark based on depth congruent melting solvent |
| CN110075154B (en) * | 2019-06-04 | 2021-06-22 | 南京林业大学 | Method for extracting saponin active ingredients from black wattle bark based on deep eutectic solvent |
| CN113181683A (en) * | 2021-05-12 | 2021-07-30 | 山东宏济堂制药集团股份有限公司 | Radix pseudostellariae standard control substance and separation and purification method and detection method thereof |
| CN116332894A (en) * | 2023-03-22 | 2023-06-27 | 广东丸美生物技术股份有限公司 | Method for preparing cocoa extract and cosmetic |
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