CN107927516A - A kind of food bacteriostatic agent and preparation method thereof - Google Patents
A kind of food bacteriostatic agent and preparation method thereof Download PDFInfo
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- CN107927516A CN107927516A CN201711082045.8A CN201711082045A CN107927516A CN 107927516 A CN107927516 A CN 107927516A CN 201711082045 A CN201711082045 A CN 201711082045A CN 107927516 A CN107927516 A CN 107927516A
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- bacteriostatic agent
- food
- food bacteriostatic
- aloe
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- 235000013305 food Nutrition 0.000 title claims abstract description 48
- 239000000022 bacteriostatic agent Substances 0.000 title claims abstract description 44
- 238000002360 preparation method Methods 0.000 title abstract description 7
- 230000000844 anti-bacterial effect Effects 0.000 claims abstract description 21
- 241001116389 Aloe Species 0.000 claims abstract description 16
- 235000011399 aloe vera Nutrition 0.000 claims abstract description 16
- 235000013717 Houttuynia Nutrition 0.000 claims abstract description 10
- 241001646826 Isodon rubescens Species 0.000 claims abstract description 10
- 244000223014 Syzygium aromaticum Species 0.000 claims abstract description 10
- 235000016639 Syzygium aromaticum Nutrition 0.000 claims abstract description 10
- 235000005187 Taraxacum officinale ssp. officinale Nutrition 0.000 claims abstract description 6
- 240000001949 Taraxacum officinale Species 0.000 claims abstract description 4
- 240000000691 Houttuynia cordata Species 0.000 claims abstract 4
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims description 24
- 239000012535 impurity Substances 0.000 claims description 18
- 239000012141 concentrate Substances 0.000 claims description 17
- 239000000706 filtrate Substances 0.000 claims description 14
- 239000000243 solution Substances 0.000 claims description 13
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 12
- 239000007788 liquid Substances 0.000 claims description 11
- 238000001035 drying Methods 0.000 claims description 10
- 241000588626 Acinetobacter baumannii Species 0.000 claims description 7
- 239000003814 drug Substances 0.000 claims description 7
- 239000000203 mixture Substances 0.000 claims description 7
- 241000588724 Escherichia coli Species 0.000 claims description 6
- 238000004821 distillation Methods 0.000 claims description 6
- 229940079593 drug Drugs 0.000 claims description 6
- 230000002949 hemolytic effect Effects 0.000 claims description 6
- 241000193830 Bacillus <bacterium> Species 0.000 claims description 5
- 241000194017 Streptococcus Species 0.000 claims description 5
- 238000001914 filtration Methods 0.000 claims description 5
- 241000894006 Bacteria Species 0.000 claims description 4
- 230000036541 health Effects 0.000 claims description 4
- 239000008155 medical solution Substances 0.000 claims description 4
- 238000002137 ultrasound extraction Methods 0.000 claims description 4
- 241000555712 Forsythia Species 0.000 claims description 3
- 241000245240 Lonicera Species 0.000 claims description 3
- 241000207834 Oleaceae Species 0.000 claims description 3
- 238000010521 absorption reaction Methods 0.000 claims description 3
- 238000010790 dilution Methods 0.000 claims description 3
- 239000012895 dilution Substances 0.000 claims description 3
- 235000013399 edible fruits Nutrition 0.000 claims description 3
- 238000010828 elution Methods 0.000 claims description 3
- 238000000605 extraction Methods 0.000 claims description 3
- 238000004064 recycling Methods 0.000 claims description 3
- 239000011347 resin Substances 0.000 claims description 3
- 229920005989 resin Polymers 0.000 claims description 3
- 239000000341 volatile oil Substances 0.000 claims description 3
- 238000009333 weeding Methods 0.000 claims description 3
- 230000037303 wrinkles Effects 0.000 claims description 3
- 241001570521 Lonicera periclymenum Species 0.000 claims description 2
- 241000245665 Taraxacum Species 0.000 claims description 2
- 238000007781 pre-processing Methods 0.000 claims description 2
- 239000008223 sterile water Substances 0.000 claims description 2
- FFRBMBIXVSCUFS-UHFFFAOYSA-N 2,4-dinitro-1-naphthol Chemical compound C1=CC=C2C(O)=C([N+]([O-])=O)C=C([N+]([O-])=O)C2=C1 FFRBMBIXVSCUFS-UHFFFAOYSA-N 0.000 claims 1
- 241000588624 Acinetobacter calcoaceticus Species 0.000 claims 1
- 244000170916 Paeonia officinalis Species 0.000 claims 1
- 235000006484 Paeonia officinalis Nutrition 0.000 claims 1
- 241000191940 Staphylococcus Species 0.000 claims 1
- 238000004519 manufacturing process Methods 0.000 abstract description 10
- 241000196324 Embryophyta Species 0.000 abstract description 7
- 244000005700 microbiome Species 0.000 abstract description 6
- 231100000252 nontoxic Toxicity 0.000 abstract description 5
- 230000003000 nontoxic effect Effects 0.000 abstract description 5
- 230000001627 detrimental effect Effects 0.000 abstract description 4
- 235000013324 preserved food Nutrition 0.000 abstract description 4
- 239000003795 chemical substances by application Substances 0.000 abstract description 3
- 238000007789 sealing Methods 0.000 abstract description 3
- 239000002778 food additive Substances 0.000 abstract description 2
- 235000013373 food additive Nutrition 0.000 abstract description 2
- 238000004321 preservation Methods 0.000 abstract description 2
- 241000205585 Aquilegia canadensis Species 0.000 abstract 1
- 239000000796 flavoring agent Substances 0.000 abstract 1
- 235000019634 flavors Nutrition 0.000 abstract 1
- 238000003860 storage Methods 0.000 abstract 1
- 239000003755 preservative agent Substances 0.000 description 10
- 230000002335 preservative effect Effects 0.000 description 9
- 241000758769 Houttuynia Species 0.000 description 6
- 241000191967 Staphylococcus aureus Species 0.000 description 5
- 239000000463 material Substances 0.000 description 5
- 238000000034 method Methods 0.000 description 5
- 239000000126 substance Substances 0.000 description 5
- 239000003643 water by type Substances 0.000 description 5
- 230000001408 fungistatic effect Effects 0.000 description 4
- 238000001514 detection method Methods 0.000 description 3
- 230000008569 process Effects 0.000 description 3
- CURLTUGMZLYLDI-UHFFFAOYSA-N Carbon dioxide Chemical compound O=C=O CURLTUGMZLYLDI-UHFFFAOYSA-N 0.000 description 2
- 241000186216 Corynebacterium Species 0.000 description 2
- 230000001775 anti-pathogenic effect Effects 0.000 description 2
- 230000001580 bacterial effect Effects 0.000 description 2
- WPYMKLBDIGXBTP-UHFFFAOYSA-N benzoic acid Chemical compound OC(=O)C1=CC=CC=C1 WPYMKLBDIGXBTP-UHFFFAOYSA-N 0.000 description 2
- 150000002148 esters Chemical class 0.000 description 2
- WQYVRQLZKVEZGA-UHFFFAOYSA-N hypochlorite Chemical compound Cl[O-] WQYVRQLZKVEZGA-UHFFFAOYSA-N 0.000 description 2
- JVTAAEKCZFNVCJ-UHFFFAOYSA-N lactic acid Chemical compound CC(O)C(O)=O JVTAAEKCZFNVCJ-UHFFFAOYSA-N 0.000 description 2
- 230000003287 optical effect Effects 0.000 description 2
- 239000000047 product Substances 0.000 description 2
- 239000005711 Benzoic acid Substances 0.000 description 1
- ZAMOUSCENKQFHK-UHFFFAOYSA-N Chlorine atom Chemical compound [Cl] ZAMOUSCENKQFHK-UHFFFAOYSA-N 0.000 description 1
- 241001478240 Coccus Species 0.000 description 1
- 229910002651 NO3 Inorganic materials 0.000 description 1
- NHNBFGGVMKEFGY-UHFFFAOYSA-N Nitrate Chemical compound [O-][N+]([O-])=O NHNBFGGVMKEFGY-UHFFFAOYSA-N 0.000 description 1
- 240000004808 Saccharomyces cerevisiae Species 0.000 description 1
- LSNNMFCWUKXFEE-UHFFFAOYSA-N Sulfurous acid Chemical compound OS(O)=O LSNNMFCWUKXFEE-UHFFFAOYSA-N 0.000 description 1
- 230000004913 activation Effects 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 235000010233 benzoic acid Nutrition 0.000 description 1
- 238000009924 canning Methods 0.000 description 1
- 239000001569 carbon dioxide Substances 0.000 description 1
- 229910002092 carbon dioxide Inorganic materials 0.000 description 1
- 238000012512 characterization method Methods 0.000 description 1
- 239000000460 chlorine Substances 0.000 description 1
- JFBJUMZWZDHTIF-UHFFFAOYSA-N chlorine chlorite Inorganic materials ClOCl=O JFBJUMZWZDHTIF-UHFFFAOYSA-N 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 230000008030 elimination Effects 0.000 description 1
- 238000003379 elimination reaction Methods 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- BEFDCLMNVWHSGT-UHFFFAOYSA-N ethenylcyclopentane Chemical compound C=CC1CCCC1 BEFDCLMNVWHSGT-UHFFFAOYSA-N 0.000 description 1
- 239000012530 fluid Substances 0.000 description 1
- 239000005452 food preservative Substances 0.000 description 1
- 235000019249 food preservative Nutrition 0.000 description 1
- 230000009545 invasion Effects 0.000 description 1
- 239000004310 lactic acid Substances 0.000 description 1
- 235000014655 lactic acid Nutrition 0.000 description 1
- 230000007774 longterm Effects 0.000 description 1
- 238000005259 measurement Methods 0.000 description 1
- 150000002826 nitrites Chemical class 0.000 description 1
- 235000015097 nutrients Nutrition 0.000 description 1
- 235000016709 nutrition Nutrition 0.000 description 1
- 230000035764 nutrition Effects 0.000 description 1
- 239000011148 porous material Substances 0.000 description 1
- 238000005057 refrigeration Methods 0.000 description 1
- 150000003839 salts Chemical class 0.000 description 1
- 239000004334 sorbic acid Substances 0.000 description 1
- 235000010199 sorbic acid Nutrition 0.000 description 1
- 229940075582 sorbic acid Drugs 0.000 description 1
- 239000003053 toxin Substances 0.000 description 1
- 231100000765 toxin Toxicity 0.000 description 1
- 238000002834 transmittance Methods 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L3/00—Preservation of foods or foodstuffs, in general, e.g. pasteurising, sterilising, specially adapted for foods or foodstuffs
- A23L3/34—Preservation of foods or foodstuffs, in general, e.g. pasteurising, sterilising, specially adapted for foods or foodstuffs by treatment with chemicals
- A23L3/3454—Preservation of foods or foodstuffs, in general, e.g. pasteurising, sterilising, specially adapted for foods or foodstuffs by treatment with chemicals in the form of liquids or solids
- A23L3/3463—Organic compounds; Microorganisms; Enzymes
- A23L3/3472—Compounds of undetermined constitution obtained from animals or plants
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Botany (AREA)
- Microbiology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Nutrition Science (AREA)
- Engineering & Computer Science (AREA)
- Food Science & Technology (AREA)
- Polymers & Plastics (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The invention belongs to food additives field, more particularly to a kind of food bacteriostatic agent and preparation method thereof, the food bacteriostatic agent includes Fructus Forsythiae, cloves, Rabdosia rubescens, cordate houttuynia, the radix paeoniae rubrathe, aloe, dandelion, rhizoma alismatis, honeysuckle, food bacteriostatic agent provided by the invention can be with the use of auxiliary anticorrosion agent, it is natural component bacteriostatic agent, it is preferably adapted for sealing canned food, it can suppress the propagation of numerous food Common detrimental microorganism, with simple production process, production cost is low, it is easy to use, it is safe and non-toxic, pure plant origin, the advantages that edible, preferably it is suitable for the storage and preservation of high-end canned food, do not influence flavor, and can be effectively antibacterial.
Description
Technical field
The present invention relates to food additives field, more particularly to a kind of food bacteriostatic agent and preparation method thereof.
Background technology
The many factors such as physics, chemistry and biology can all cause food spoilage, these factors are typically at the same time or accompany
Occur.Since food nutrition is enriched, suitable for microorganism growing multiplication, and microorganism is ubiquitous, all-pervasive, therefore carefully
The invasion and attack of the quasi-microorganism of bacterium, mould and yeast are typically to cause the main reason of food spoilage.
In industrial practice, to preserve the canning of food generally use, refrigeration, drying, marinated or chemical preservation method.With top
Method differs from one another, the quick-frozen preserving process such as developed rapidly, very superior for the quality for keeping food, but its big model
Enclose to use and limited be subject to equipment and the conditions such as cost.Under certain conditions, food preservative is a kind of cheap, operation
Supplementary means that is easy, working well, has remarkable result to the loss for preventing some perishable items.
At present, the predominantly chemical preservative from the composition of preservative and source.Chemical preservative includes organic chemistry
Preservative and two major class of inorganic chemistry preservative.Organic chemistry preservative mainly include benzoic acid and its esters, sorbic acid and its
Salt, parabens and lactic acid etc., inorganic chemistry preservative mainly include sulfurous acid and its esters, carbon dioxide,
Nitrate and nitrites, free chlorine and hypochlorite etc..Chemical preservative is one " double-edged sword ", it is possible to people's
Health care belt necessarily endangers.The preservative overwhelming majority used at present in China, food is artificial synthesized, improper use
Have certain side effect；Some preservatives even contain micro toxin, and long-term Excess free enthalpy can cause health necessarily
Infringement.
The present invention provides the natural component bacteriostatic agent that a kind of auxiliary anticorrosion agent uses, and is preferably adapted for sealing canned food
Product, can suppress the propagation of numerous food Common detrimental microorganism, have simple production process, production cost it is low, it is easy to use,
The advantages such as safe and non-toxic, pure plant origin, edible.
The content of the invention
In order to solve the above technical problem, the present invention provides a kind of food bacteriostatic agent and preparation method thereof, can suppress more
The propagation of kind of food Common detrimental microorganism, has that simple production process, production cost be low, nontoxic, pure plant easy to use, safe
The advantages such as thing source, edible.
To achieve the above object, the present invention provides a kind of food bacteriostatic agent, the food bacteriostatic agent include Fructus Forsythiae, cloves,
Rabdosia rubescens, cordate houttuynia, the radix paeoniae rubrathe, aloe.
Preferably, the food bacteriostatic agent further includes dandelion, rhizoma alismatis, honeysuckle.
Further, according to any food bacteriostatic agent of claim 1 or 2, it is characterised in that the making food
The step of bacteriostatic agent, includes：The drying plumule of Oleaceae plants Fructus Forsythiae is chosen, takes crude drug to be smashed to pieces after removing impurity, is ground to
40-60 mesh；Corynebacterium cloves fruit is taken, preferably ground surface is rufous or purple brown, has thin wrinkle, takes crude drug to remove impurity elimination
Matter, smashs to pieces after weeding out ash bits, is ground to 40-60 mesh；Take Rabdosia rubescens to remove impurity, weed out grey bits, smash to pieces, grind after segment drying
To 40-60 mesh；Cordate houttuynia when taking the luxuriant flower spike of summer cauline leaf more is tapped, and is dried after removing impurity, is weeded out grey bits, smash after segment
It is broken, it is ground to 40-60 mesh；Take the radix paeoniae rubrathe to remove impurity, clean and moisturize, cut sheet, remove drying after impurity, smash after weeding out ash bits
It is broken, it is ground to 40-60 mesh；Aloe health stalk 200g is taken, is impregnated 60 minutes in 75% ethanol solution of 10 times of weight,
Filtering obtains the first filtrate after being extracted 30 minutes with the power ultrasonic of 4000W under conditions of 40 DEG C；Aloe filter residue is again with 10 times of weights
60% ethanol solution ultrasonic extraction of amount, after being filtered after being extracted 30 minutes with the power ultrasonic of 4000W under conditions of 40 DEG C
Obtain the second filtrate, it is filtrate mixture to merge the first filtrate with the second filtrate, by filtrate mixture distillation and concentration to relatively close
Spend for 1.1-1.3, obtain extract solution from aloe；The above-mentioned radix paeoniae rubrathe of the extract solution from aloe 50ml and 130g, the above-mentioned Fructus Forsythiaes of 50g, 25g are above-mentioned
The above-mentioned Rabdosia rubescens of cloves, 90g, the above-mentioned cordate houttuynias of 10g add the sterile water of 10 times of amounts, when distillation extraction 6 is small, collect volatile oil, medicine
Liquid filtration, which preserves, obtains admixing medical solutions；The dregs of a decoction add water to cook twice with 10g dandelions, 9g rhizoma alismatis, 30g honeysuckles, and one is small every time
When, for the first time plus 10 times of amount water, second plus 5 times of amount water, obtain decocting liquid, decocting liquid and admixing medical solutions are merged into pre-process it is antibacterial
Concentrate, upper processed good LD605 type resin columns cross column, after pre-processing antibacterial concentrate absorption, add 70% ethanol
Elution, recycling ethanol obtain antibacterial concentrate, food bacteriostatic agent are obtained after the antibacterial concentrate dilution.
Preferably, the food bacteriostatic agent preferably dilutes 10 times -20 times when in use.
It is highly preferred that the food bacteriostatic agent preferably dilutes 10 times when in use.
Further, the food bacteriostatic agent can suppress most of canned common bacteria preserved in food.
Further, the food bacteriostatic agent can suppress the B-mode chain of staphylococcus aureus, Escherichia coli, hemolytic
Coccus, Nocard's bacillus, Acinetobacter baumannii.
Preferably, the food bacteriostatic agent can suppress the propagation of Acinetobacter baumannii.
" OD " of the present invention is the abbreviation of optical density (optical density), represents the light that detected material sponges
Density, is the proper noun in detection method, and detection unit is represented with OD values, 1OD=log10 (1/trans), wherein trans
For the light transmittance T values of detectable substance.For light by detected material, front and rear capacity volume variance is the energy that detected material sponges, special
Under standing wave length, the concentration of same detected material and absorbed energy are into quantitative relationship, preferably in embodiments of the present invention,
The numerical value of OD600 can term characterization bacterial concentration, that is, bacterial number.
The beneficial effects of the invention are as follows：
The present invention provides the natural component bacteriostatic agent that a kind of auxiliary anticorrosion agent uses, and is preferably adapted for sealing canned food
Product, can suppress the propagation of numerous food Common detrimental microorganism, have simple production process, production cost it is low, it is easy to use,
The advantages such as safe and non-toxic, pure plant origin, edible.
Brief description of the drawings
Fig. 1 is the fungistatic effect detects schematic diagram of food bacteriostatic agent provided by the invention.
Embodiment
To make the object, technical solutions and advantages of the present invention clearer, the present invention is made into one below in conjunction with attached drawing
It is described in detail on step ground.
Embodiment 1, antipathogenic composition are chosen
Fructus Forsythiae, cloves, Rabdosia rubescens, cordate houttuynia, the radix paeoniae rubrathe, aloe are chosen as antipathogenic composition, the material selection and pre- place
Reason process is as follows：
Fructus Forsythiae：The drying plumule of Oleaceae plants Fructus Forsythiae is chosen, takes crude drug, is removed and is smashed to pieces after impurity, is ground to 40-60
Mesh；
Cloves：Corynebacterium fruit is taken, preferably ground surface is rufous or purple brown, has thin wrinkle, takes crude drug, is removed
Impurity, weeds out after ash is considered to be worth doing and smashs to pieces, is ground to 40-60 mesh；
Rabdosia rubescens：Impurity is removed, grey bits is weeded out, is smashed to pieces after segment drying, is ground to 40-60 mesh；
Cordate houttuynia：The luxuriant flower spike of summer cauline leaf is tapped when more, is dried after removing impurity, is weeded out grey bits, smash to pieces, grind after segment
It is milled to 40-60 mesh；
The radix paeoniae rubrathe：Removing impurity, cleans and moisturizes, and cuts sheet, removes drying after impurity, weeds out after ash is considered to be worth doing and smashs to pieces, is ground to 40-
60 mesh；
Aloe：Take healthy stalk.
The preparation of embodiment 2, natural component bacteriostatic agent
The preparation of natural component bacteriostatic agent comprises the following steps：
1) 200g aloes are taken, impregnate 60min with 75% ethanol solution of 10 times of weight, under conditions of 40 DEG C with
Filtered after the power ultrasonic extraction 30min of 4000W；Filter residue is again with 60% ethanol solution ultrasonic extraction of 10 times of weight, condition
Ibid, filter, filtrate is spare twice for merging, and it is 1.0-1.5 that filtrate, which is concentrated into relative density, preferably 1.1-1.3, obtains aloe
Extracting solution；
2) the 130g radix paeoniae rubrathe, 50g Fructus Forsythiaes, 25g cloves, 90g Rabdosia rubescens, 10g cordate houttuynias and above-mentioned extract solution from aloe 50ml are taken
Add the water of 10 times of amounts, when distillation extraction 6 is small, collects volatile oil, take another container to be used to preserve after liquid filtration；The dregs of a decoction and 10g
Dandelion, 9g rhizoma alismatis, 30g honeysuckles add water to cook twice, one hour every time, and for the first time plus 10 times are measured water, second plus 5 times of amounts
Water, collecting decoction simultaneously merge with liquid after distillation, upper processed good large pore resin absorption column, after liquid adsorbs, add
70% ethanol elution, recycling ethanol obtain antibacterial concentrate.
Embodiment 3, the detection of bacteriostatic agent fungistatic effect
Buy commercially available strain staphylococcus aureus, Escherichia coli, hemolytic beta streptococcus, Nocard's bacillus, Boydii not
Lever bacterium, it is spare after activation, take 250ml triangular flasks 15,50ml LB fluid nutrient mediums are added in each bottle, points five groups (A1,
A2, CK1；B1, B2, CK2；C1, C2, CK3；D1, D2, CK4；E1, E2, CK5), 1g staphylococcus aureuses are inoculated with A1 and are added
Enter the antibacterial concentrate that 1ml dilutes 10 times, be inoculated with 1g staphylococcus aureuses in A2 and add the antibacterial concentration that 1ml dilutes 20 times
Liquid, CK1 add 1ml sterile waters；1g Escherichia coli are inoculated with B1 and add the antibacterial concentrate that 1ml dilutes 10 times, are inoculated with B2
1g Escherichia coli simultaneously add the antibacterial concentrate that 1ml dilutes 20 times, and CK2 adds 1ml sterile waters；It is B-mode that 1g hemolytics are inoculated with C1
Streptococcus simultaneously adds the antibacterial concentrate that 1ml dilutes 10 times, is inoculated with 1g hemolytics beta streptococcus in C2 and adds 1ml dilutions 20
Antibacterial concentrate again, CK3 add 1ml sterile waters；1g Nocard's bacillus is inoculated with D1 and adds the antibacterial concentration that 1ml dilutes 10 times
Liquid, 1g Nocard's bacillus is inoculated with D2 and adds the antibacterial concentrate that 1ml dilutes 20 times, CK4 adds 1ml sterile waters；It is inoculated with E1
1g Acinetobacter baumanniis simultaneously add 1ml and dilute 10 times of antibacterial concentrate, and 1g Acinetobacter baumanniis are inoculated with E2 and to add 1ml dilute
20 times of antibacterial concentrate is released, CK5 adds 1ml sterile waters；Above experimental group is put into 28 DEG C of shaking table and cultivates five days, and daily 18 points take
Sample, measurement OD600 values simultaneously record, as shown in table 1 and Fig. 1.
Table 1, food bacteriostatic agent antibacterial effect testing result
From table 1 and Fig. 1, food bacteriostatic agent provided by the invention is to staphylococcus aureus, Escherichia coli, hemolytic
Beta streptococcus, Acinetobacter baumannii have certain fungistatic effect, wherein, this hair notable to the fungistatic effect of Acinetobacter baumannii
The bacteriostatic agent of bright offer preferably dilutes ten times when in use, bacteriostatic agent simple production process provided by the invention, production cost
Nontoxic, pure plant origin low, easy to use, safe, edible.
The above disclosure is only the preferred embodiments of the present invention, cannot limit the right model of the present invention with this certainly
Enclose, therefore equivalent variations made according to the claims of the present invention, it is still within the scope of the present invention.
Claims (7)
1. a kind of food bacteriostatic agent, it is characterised in that the food bacteriostatic agent includes Fructus Forsythiae, cloves, Rabdosia rubescens, cordate houttuynia, red
Chinese herbaceous peony, aloe.
2. food bacteriostatic agent according to claim 1, the food bacteriostatic agent further includes dandelion, rhizoma alismatis, honeysuckle.
3. according to any food bacteriostatic agent of claim 1 or 2, it is characterised in that the step for making food bacteriostatic agent
Suddenly include：The drying plumule of Oleaceae plants Fructus Forsythiae is chosen, takes crude drug to be smashed to pieces after removing impurity, is ground to 40-60 mesh；Take short
Bar-shaped cloves fruit, preferably ground surface are rufous or purple brown, there is thin wrinkle, take crude drug to remove impurity, weed out grey bits
After smash to pieces, be ground to 40-60 mesh；Take Rabdosia rubescens to remove impurity, weed out grey bits, smashed to pieces after segment drying, be ground to 40-60 mesh；
Cordate houttuynia when taking the luxuriant flower spike of summer cauline leaf more is tapped, and is dried after removing impurity, is weeded out grey bits, smash to pieces, be ground to after segment
40-60 mesh；Take the radix paeoniae rubrathe to remove impurity, clean and moisturize, cut sheet, remove drying after impurity, smash to pieces, be ground to after weeding out ash bits
40-60 mesh；Aloe health stalk 200g is taken, is impregnated 60 minutes in 75% ethanol solution of 10 times of weight, in 40 DEG C of bar
Filtering obtains the first filtrate after being extracted 30 minutes with the power ultrasonic of 4000W under part；Aloe filter residue is again with the 60% of 10 times of weight
Ethanol solution ultrasonic extraction, obtain second after being filtered after being extracted 30 minutes with the power ultrasonic of 4000W under conditions of 40 DEG C
Filtrate, it is filtrate mixture to merge the first filtrate with the second filtrate, is 1.1- by filtrate mixture distillation and concentration to relative density
1.3, obtain extract solution from aloe；The extract solution from aloe 50ml and the above-mentioned radix paeoniae rubrathe of 130g, the above-mentioned Fructus Forsythiaes of 50g, the above-mentioned cloves of 25g, 90g
The above-mentioned cordate houttuynia of above-mentioned Rabdosia rubescens, 10g adds the sterile water of 10 times of amounts, when distillation extraction 6 is small, collects volatile oil, liquid filtration is protected
Deposit acquisition admixing medical solutions；The dregs of a decoction add water to cook twice with 10g dandelions, 9g rhizoma alismatis, 30g honeysuckles, one hour every time, for the first time
Adding 10 times of amount water, second plus 5 times of amount water, obtain decocting liquid, decocting liquid and admixing medical solutions are merged into the antibacterial concentrate of pretreatment, on
Processed good LD605 type resin columns cross column, after pre-processing antibacterial concentrate absorption, add 70% ethanol elution, recycling
Ethanol obtains antibacterial concentrate, and food bacteriostatic agent is obtained after the antibacterial concentrate dilution.
4. food bacteriostatic agent according to claim 3, it is characterised in that the food bacteriostatic agent is preferable dilute when in use
Release 10 times -20 times.
5. according to any food bacteriostatic agents of claim 1-4, it is characterised in that the food bacteriostatic agent can suppress big
The canned common bacteria preserved in food in part.
6. according to any food bacteriostatic agent of claim 5, it is characterised in that the food bacteriostatic agent can suppress golden yellow
Color staphylococcus, Escherichia coli, hemolytic beta streptococcus, Nocard's bacillus, Acinetobacter baumannii.
7. food bacteriostatic agent according to claim 6, it is characterised in that the food bacteriostatic agent is preferably able to suppress Bao
The propagation of family name's acinetobacter calcoaceticus.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201711082045.8A CN107927516A (en) | 2017-11-07 | 2017-11-07 | A kind of food bacteriostatic agent and preparation method thereof |
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| CN108578476A (en) * | 2018-05-31 | 2018-09-28 | 广州市普精生物科技有限公司 | A kind of composition and its preparation method and application of prevention fish nocardiasis |
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| CN109845795A (en) * | 2018-12-14 | 2019-06-07 | 马山县山山农业科技有限公司 | Bacteriostasis method is sterilized applied to the beef in slaughtering process |
| CN112400975A (en) * | 2020-11-18 | 2021-02-26 | 烟台喜旺肉类食品有限公司 | Method for removing extravasated blood from pig head |
| CN112603863A (en) * | 2020-12-21 | 2021-04-06 | 河南省济源市济世药业有限公司 | Rabdosia rubescens low-boiling-point volatile matter, extraction method and application thereof, and mosquito repelling product |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN108578476A (en) * | 2018-05-31 | 2018-09-28 | 广州市普精生物科技有限公司 | A kind of composition and its preparation method and application of prevention fish nocardiasis |
| CN108902645A (en) * | 2018-06-29 | 2018-11-30 | 苏州汉德瑞生物工程有限公司 | A kind of plant bacteriostatic agent and preparation method thereof |
| CN109845795A (en) * | 2018-12-14 | 2019-06-07 | 马山县山山农业科技有限公司 | Bacteriostasis method is sterilized applied to the beef in slaughtering process |
| CN112400975A (en) * | 2020-11-18 | 2021-02-26 | 烟台喜旺肉类食品有限公司 | Method for removing extravasated blood from pig head |
| CN112603863A (en) * | 2020-12-21 | 2021-04-06 | 河南省济源市济世药业有限公司 | Rabdosia rubescens low-boiling-point volatile matter, extraction method and application thereof, and mosquito repelling product |
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