CN107926951B

CN107926951B - Bactericidal composition containing terpene alcohol and trifloxystrobin and application thereof

Info

Publication number: CN107926951B
Application number: CN201711334235.4A
Authority: CN
Inventors: 高燕; 刘明津; 杨彩宏; 田兴山
Original assignee: Plant Protection Research Institute Guangdong Academy of Agricultural Sciences
Current assignee: Plant Protection Research Institute Guangdong Academy of Agricultural Sciences
Priority date: 2017-12-12
Filing date: 2017-12-12
Publication date: 2020-07-14
Anticipated expiration: 2037-12-12
Also published as: CN107926951A

Abstract

The invention discloses a bactericidal composition containing terpene alcohol and trifloxystrobin, wherein active ingredients in the bactericidal composition at least comprise the terpene alcohol and the trifloxystrobin, and the weight ratio of the terpene alcohol to the trifloxystrobin is (1: 30-10): 1, preferably 1:10-5: 1; after being compounded, the bactericidal composition shows a certain synergistic effect on the prevention and the treatment of crop diseases; the bactericidal composition is processed into any one dosage form suitable for agricultural production by being supplemented with auxiliary components commonly used in pesticide preparation processing, and the dosage form comprises but is not limited to one of missible oil, wettable powder, water dispersible granules, suspending agents, microemulsion, emulsion in water and microcapsule suspending agents. The bactericidal composition is safe to crops, has good control effect and is worthy of popularization and application.

Description

Bactericidal composition containing terpene alcohol and trifloxystrobin and application thereof

Technical Field

The invention relates to the technical field of pesticides, in particular to a bactericidal composition containing terpene alcohol and trifloxystrobin and application thereof in preventing and treating crop diseases.

Background

Terpene alcohol, named tea tree oil in English, is a melaleuca alternifolia extract, is a novel biological bactericide developed by Stockton in Israel, and has good control effect on various diseases such as downy mildew, powdery mildew, early blight, late blight, leaf spot and the like of various crops; the safety to crops and environment is high, the usage amount of field pesticides can be greatly reduced, and the environment protection is facilitated. At present, registration is obtained in more than 30 countries in the world, and the registration is used for preventing and controlling powdery mildew and epidemic disease in China.

The trifloxystrobin belongs to trifloxystrobin broad-spectrum bactericides, is a novel fluorine-containing bactericide successfully developed by taking natural products Strobilurins as bactericide lead compounds, has the characteristics of high efficiency, broad spectrum, protection, treatment, eradication, permeation, systemic activity, rain wash resistance, long duration and the like, is effective on strains which generate resistance to 1, 4-demethylated mildew inhibitors, benzamides, dicarboxamides and benzomicazole, has no cross resistance with the existing bactericides, has good activity on almost all fungal (ascomycetes, basidiomycetes, oomycetes and fungi imperfecti) diseases such as powdery mildew, leaf spot, rust disease, downy mildew, rhizoctonia solani and the like, and can be quickly degraded in soil and water, so the bactericide is safe to the environment.

In order to solve the practical factors such as the reduction of the control effect caused by the resistance problem, compounding different pesticide active ingredient varieties is a hot direction for pesticide research. Terpene alcohol has the characteristics of multifunction, broad spectrum, safety and the like, and particularly has high compatibility with most chemical pesticides, so that the terpene alcohol becomes a biological pesticide with great value. Through retrieval, no related report about the combination of the terpene alcohol and the trifloxystrobin is found.

Disclosure of Invention

Based on the above, one of the purposes of the invention is to provide a bactericidal composition containing terpene alcohol and trifloxystrobin, wherein the bactericidal composition can achieve a certain synergistic effect within a certain proportion range; meanwhile, the bactericidal composition can expand the bactericidal spectrum, delay the generation of drug resistance and prolong the service life of products.

Another object of the present invention is to provide an application of the bactericidal composition in the preparation of a medicament for controlling crop diseases including, but not limited to, powdery mildew, downy mildew, late blight, leaf spot, rust disease, damping off, or sclerotinia.

It is still another object of the present invention to provide a method for controlling diseases of crops, which comprises applying the plant and the locus where the plant is growing, before or after the plant disease is germinated, by conventional methods such as soil mixing, spraying, pouring and the like, at an application rate depending on the climatic field or the state of the plant, and at a bactericidal effect generally closely related to external factors such as climate, the degree of occurrence of the disease and the like.

In order to achieve the purpose, the invention adopts the following technical scheme:

the active ingredients of the bactericidal composition at least comprise terpene alcohol and trifloxystrobin, wherein the weight ratio of the terpene alcohol to the trifloxystrobin is 1:30-10:1, preferably, the weight ratio is 1:10-5: the control effect is better when the weight ratio is 4:1, and the control effect is optimal when the weight ratio is 4: 1.

Furthermore, the content of the terpene alcohol and the trifloxystrobin in the bactericidal composition is 1-80% by weight, preferably 10-60% by weight, and the balance is auxiliary agents, carriers and/or other known substances which are helpful for the stability of the effective components in storage and use and the exertion of the drug effect.

Furthermore, the bactericidal composition can be processed into any one dosage form suitable for agricultural production by a conventional method, namely by being supplemented with auxiliary components commonly used in pesticide preparation processing, and the dosage form comprises but is not limited to one of missible oil, wettable powder, water dispersible granules, suspending agents, microemulsions, aqueous emulsions and microcapsule suspending agents.

Further, the germicidal composition may be provided in the form of a finished formulation, i.e., the materials in the composition have been mixed; or in separate formulations which are self-mixing in a tub or tank prior to use and optionally diluted with water depending on the concentration of active desired.

Further, the bactericidal composition may further contain other agricultural active ingredients, including but not limited to one or more of other kinds of pesticides, bactericides, nematicides, synergists, fertilizers, safeners and plant growth regulators.

Compared with the prior art, the bactericidal composition has the beneficial effects that:

1. after the bactericidal composition is compounded according to the proportion, the control effects of the bactericidal composition are not simple superposition, but show a certain synergistic effect;

2. the active ingredients in the bactericidal composition have different action mechanisms, and the combination of the active ingredients and the bactericidal composition can delay the drug resistance of pathogenic bacteria, thereby being beneficial to prolonging the service life of the pesticide active ingredients;

3. the bactericidal composition can reduce the usage amount of pesticide active ingredients and the use cost, and meanwhile, the bactericidal composition is safe to crops and is environment-friendly and harmless.

Detailed Description

In order to make the purpose and technical solution of the present invention more clear, the present invention is further described with reference to the following specific embodiments, it should be noted that the specific embodiments are only for explaining the present invention, and not for limiting the present invention, and the non-inventive changes made according to the specific embodiments of the present invention by those skilled in the art based on the description of the present invention should be considered as the protection scope of the present invention, which is defined by the claims.

First, the embodiments of bioassay

1. Test subjects: tomato late blight pathogen;

1. 2, testing method, using hypha growth rate method to measure, dissolving terpene alcohol and trifloxystrobin with solvent, using sterile water to prepare mother liquor with concentration of 1000ug/m L, using sterile water to prepare a series of concentration gradients and different proportions to mix, mixing with rye sucrose agar culture medium cooled to about 60 ℃ according to volume ratio of 1:9 to prepare a culture medium-containing plate, fetching fungus cake with diameter of 5mm from the edge of tomato late blight bacterium colony pre-cultured for 7 days on the rye sucrose agar culture medium plate, inoculating the hypha face downwards to the center of the drug-containing culture medium plate, 1 fungus dish per dish, repeating 3 times per treatment, placing in a constant temperature incubator at 18 ℃ to culture, measuring colony diameter by cross method after 7 days, calculating average value of colony diameter and hypha growth inhibition rate, using DPS statistical software to calculate effective inhibition concentration EC of each medicament on tomato late blight bacterium₅₀And calculating the co-toxicity coefficient according to the Sun cloud Peel method.

When the CTC is less than or equal to 80, the composition shows antagonism, when the CTC is more than 80 and less than 120, the composition shows additive action, when the CTC is more than or equal to 120, the composition shows synergy, and the indoor toxicity measurement result is shown in the table 1:

TABLE 1 indoor Activity measurement of terpene alcohol and Trifloxystrobin combination for tomato late blight

As can be seen from the table 1, the combination of the terpene alcohol and the trifloxystrobin in the ratio range of 1:30-10:1 has the co-toxicity coefficient of over 120 for preventing and treating the tomato late blight, and shows the synergistic effect. Especially, when the compounding ratio of the two is 1:10-5:1, the co-toxicity coefficient is more than 155, and the synergistic effect is more prominent.

Second, preparation examples

1. EXAMPLE 1 50% wettable powder of terpene alcohol trifloxystrobin (1:1)

The preparation method of the wettable powder comprises the following steps: the active ingredients and the pesticide auxiliary ingredients are uniformly mixed according to the formula in proportion, and the mixture is crushed by an ultrafine crusher to obtain the wettable powder.

2. Example 2 40% terpene alcohol trifloxystrobin suspending agent (4:1)

The preparation method of the suspending agent comprises the following steps: the suspending agent is prepared by uniformly mixing other auxiliary agents, uniformly mixing the auxiliary agents through high-speed shearing, adding the effective components of terpene alcohol and trifloxystrobin, and grinding the mixture in a ball mill for more than 3 hours to ensure that the particle size of the preparation is below 5 microns.

3. Example 3: 36% terpene alcohol-trifloxystrobin suspending agent (5:1)

4. Example 4 terpene alcohol trifloxystrobin Water dispersible granules 44% (1:10)

The preparation method of the water dispersible granule comprises the following steps: the water dispersible granule is prepared by uniformly mixing the active ingredients, the auxiliary agent, the disintegrating agent, the filler and the like according to the formula, crushing the mixture by an ultramicro jet mill, adding a proper amount of water to granulate on a granulator, and drying the granules.

Third, field drug effect test

1. The control object is: late blight of tomato;

2. the test method comprises the steps of carrying out the test on a tomato greenhouse in the hilly region of Jinan city, wherein the tomato variety is the zida power, tomato epidemic diseases occur all the year round, using the pesticide composition preparation of the embodiment 1-4 as a test medicament, using 9% terpene alcohol missible oil and 50% trifloxystrobin water dispersible granules as control medicaments, wherein the area of each cell is about 15 square meters, randomly arranging the blocks, and repeating the treatment for 4 times, and spraying by using a Jacto HD400 type manual sprayer when the late blight occurs sporadically, wherein the using amount of the liquid medicine is 900L/hm²Uniformly spraying, carrying out first application at the initial stage of disease onset, carrying out second application 7 days later, investigating the disease condition base before application, investigating the prevention and treatment effect 7 days before and after second application, wherein the test results are shown in Table 2:

TABLE 2 field efficacy test of different agent treatments on tomato late blight

Test agent	The effective component dosage (g/hectare)	First control effect/%)	Second control effect/%)
				Example 1	120	79.3	91.5
Example 2	120	89.6	97.2
				Example 3	120	81.9	93.6
Example 4	120	76.2	89.4
				9% terpene alcohol emulsifiable concentrate	90	56.7	79.4
50% trifloxystrobin water dispersible granule	90	51.4	72.8

The field efficacy test results are shown in table 2, and it can be seen from table 2 that the control effect of the compound pesticide composition of terpene alcohol and trifloxystrobin on tomato late blight is obviously better than that of the control single agent; in addition, no phytotoxicity to tomato crops is observed in the application range, which shows that the bactericidal composition is good in safety and worthy of popularization and application.

Claims

1. The suspending agent containing terpene alcohol and trifloxystrobin is characterized by comprising the following components:

terpene alcohol 32%

Trifloxystrobin 8%

Alkylphenol polyoxyethylene ether 3%

Polyoxyethylene ether phosphate 4%

0.1 percent of magnesium aluminum silicate

Xanthan gum 0.1%

0.3 percent of organic silicone

Sodium benzoate 0.2%

3 percent of ethylene glycol

The balance of deionized water.

2. The suspending agent according to claim 1, which is prepared by a method comprising: the suspending agent is prepared by uniformly mixing other auxiliary agents, uniformly mixing the auxiliary agents through high-speed shearing, adding the effective components of terpene alcohol and trifloxystrobin, and grinding the mixture in a ball mill for more than 3 hours to ensure that the particle size of the preparation is below 5 microns.

3. Use of the suspension concentrate according to claim 1 for the control of tomato late blight.