CN107926430A - A kind of high-yield method of shatian pomelo - Google Patents
A kind of high-yield method of shatian pomelo Download PDFInfo
- Publication number
- CN107926430A CN107926430A CN201711174223.XA CN201711174223A CN107926430A CN 107926430 A CN107926430 A CN 107926430A CN 201711174223 A CN201711174223 A CN 201711174223A CN 107926430 A CN107926430 A CN 107926430A
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- China
- Prior art keywords
- plant
- shatian pomelo
- endophyte
- yellow mud
- bacillus amyloliquefaciens
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Classifications
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G17/00—Cultivation of hops, vines, fruit trees, or like trees
- A01G17/005—Cultivation methods
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G7/00—Botany in general
- A01G7/06—Treatment of growing trees or plants, e.g. for preventing decay of wood, for tingeing flowers or wood, for prolonging the life of plants
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
Abstract
The invention discloses a kind of high-yield method of shatian pomelo, include the following steps：The inoculated plant endophyte into shatian pomelo plant；The inoculation method is：Yellow mud is chosen as base-material, the percent water for adjusting yellow mud adds water-retaining agent and endophyte of plant into yellow mud for 20%~25%, stirs evenly, then after the root of yellow mud is wrapped in the root of shatian pomelo plant, cultivated two days, then transplanted using the radiation of visible light of 520~670nm of wavelength；The endophyte of plant is made of bacillus amyloliquefaciens, citric acid bacillus, the raw Erwinia of grass, acid-producing Klebsiella bacterium, Pseudomonas fluorescens；On the basis of yellow mud, the additive amount of the endophyte of plant is 5~15g/Kg.Artificial inoculated plant endophyte before shatian pomelo plantation of the present invention, improves the disease-resistant function of shatian pomelo, can improve shatian pomelo disease resistance, promote the growth of shatian pomelo, so as to improve the yield of shatian pomelo.
Description
Technical field
The present invention relates to planting fruit trees technical field, and in particular to a kind of high-yield method of shatian pomelo.
Background technology
Shatian pomelo has the characteristics that big fruit, good quality, storage endurance, easily transport and market are wide, and shatian pomelo is in growth course
In, the appearance of pest and disease damage not only reduces the survival rate of shatian pomelo, can also reduce the yield of shatian pomelo.
Shatian pomelo is adapted to be grown in the good soil of loose, fertile, moistening, aeration, the moisture requirement of soil compared with
Height, is easily tortured by disease pest in shatian pomelo planting process, thus is caused under shatian pomelo low output, and the disease of shatian pomelo mainly has routed
Ulcer disease, epidemic disease, yellow twig, longicorn, scale insect, leaf miner and acarid etc., wherein canker occurs mainly in the leaf of shatian pomelo
On trunk, stay glue disease to occur mainly in epidemic disease on the trunk of shatian pomelo and occur mainly on the fruit of shatian pomelo, yellow twig is
Communicable disease, it is found that yellow twig must eradicate shatian pomelo in time, prevent disease spread；And longicorn mainly appears on shatian pomelo
Limb, scale insect mainly appeared on the limb and fruit of shatian pomelo, and leaf miner mainly appears on the taper of shatian pomelo, acarid
Mainly it is grown on the pericarp and leaf of shatian pomelo, the disease position of shatian pomelo is different, and the method for use is essentially in phase
Corresponding pesticide is sprayed at the shatian pomelo position answered, and shatian pomelo pest species are more, and increase can the shatian pomelo techniques of pest control
Difficulty.
The content of the invention
Harm a kind of difficult, the technical problem of shatian pomelo low output, there is provided shatian pomelo the present invention overcomes shatian pomelo disease pest
High-yield method.
To solve the above problems, the present invention adopts the following technical scheme that：
A kind of high-yield method of shatian pomelo, includes the following steps：
The inoculated plant endophyte into shatian pomelo plant；The inoculation method is：Yellow mud is chosen as base-material, adjusts yellow mud
Percent water water-retaining agent and endophyte of plant are added into yellow mud for 20%~25%, stir evenly, then by yellow mud
After root is wrapped in the root of shatian pomelo plant, cultivated two days using the radiation of visible light of 520~670nm of wavelength, then transplant；It is described
Endophyte of plant it is false singly by bacillus amyloliquefaciens, citric acid bacillus, the raw Erwinia of grass, acid-producing Klebsiella bacterium, fluorescence
Born of the same parents bacterium forms；On the basis of yellow mud, the additive amount of the endophyte of plant is 5~15g/Kg.
Wherein, the endophyte of plant is 20 by viable count quantity ratio:1~4：3~5:3~6:9~10 solution starch
Bacillus, citric acid bacillus, the raw Erwinia of grass, acid-producing Klebsiella bacterium, Pseudomonas fluorescens composition.
Wherein, the bacillus amyloliquefaciens also carry out the antibacterial domestication of following steps:Bacillus amyloliquefaciens are put
Ash arrhizus bacteria, wilt are added into culture medium after 1~2d is cultivated in culture medium, continues fermentation to the grey mold in culture medium
When germ, wilt all kill, pick out bacillus amyloliquefaciens and obtain the bacillus amyloliquefaciens；Described
Culture medium prescription is beef extract 6g/L, potato 13g/L, glucose 5g/L, sodium chloride 5g/L, ammonium chloride 5g/L.
Wherein, when the ash arrhizus bacteria in the culture medium, wilt all kill, the total enzyme activity of culture medium is not less than
832U/ml。
Wherein, the water-retaining agent is made of konjak starch, sodium acrylate.
Wherein, the intensity of illumination of the visible ray is 800~1100Lux.
Bacillus amyloliquefaciens can produce the antibiotic and antibacterial protein isoreactivity material of low molecular weight, and secrete yellow mould
Element, azophenlyene carboxylic acid, Teng Hua pyos, pyrrolnitrin isoreactivity material can be to phytopathogens, bacterium, fungi, virus
Nematode plays inhibitory action；The growth that antibacterial material suppresses harmful pathogen can also be produced by assimilation by solving starch cell enzyme
Or pathogen is directly killed, bacillus amyloliquefaciens can also produce extracellular hydrolase, also function to inhibitory action to pathogen, it can
Produce superoxide dismutase, add phenol oxidase, catalase etc., improve the resistance of crop, it is quick to can induce plant
Endogenous auxin is secreted, such as gibberellin, heteroauxin, the basic element of cell division promote crop quickly to grow.It presses down with antibacterial at the same time
Bacterium acts on, and has significant preventive effect to a variety of germs such as ash arrhizus bacteria, wilt.Bacillus amyloliquefaciens can be in fruits and vegetables wound
The nutriment that simultaneously amount reproduction absorbs plants wound is grown, pathogen is cannot get nutriment and is suppressed the life of pathogenic bacteria
It is long.When plant is mechanically damaged or when infecting of pathogen, bacillus amyloliquefaciens can induce plant in plant
Produce nature defensive enginery.
Citric acid bacillus can also produce hydrolase, chitinase and dextranase.The cell membrane main ingredient of plant epiphyte
For chitin and glucan, these hydrolases can reach diseases prevention with the cell membrane of degradative fungi or the other factor such as toxin etc. of curing the disease
Effect.The citric acid bacillus that the present invention uses are isolated from olive fruits.
The raw Erwinia of grass and acid-producing Klebsiella bacterium can secrete heteroauxin and the basic element of cell division, can promote shatian pomelo
Growth.
Pseudomonas fluorescens can secrete yellow green yellow green and bite iron element, and iron can be competed with pathogen, is being grown in pathogen
During cannot get enough nutrients and dead.
The endophyte of plant that the present invention adds can also produce a variety of alkaloids, have to a variety of herbivores and insect and poison
Effect.
The present invention has the advantages that compared with prior art：
(1) artificial inoculated plant endophyte before shatian pomelo plantation of the present invention, improves the disease-resistant function of shatian pomelo, can improve sand
Field shaddock disease resistance, promotes the growth of shatian pomelo, so as to improve the yield of shatian pomelo.
(2) after the present invention soaks endophyte of plant to shatian pomelo, 520~670nm of wavelength is used with 800~1100Lux light
According to intensity irradiance shatian pomelo plant, the light energy of the 520~670nm of wavelength is suitable, and 800~1100Lux intensities of illumination are fitted
Preferably, under this illumination condition, shatian pomelo plant can carry out low intensive photosynthesis, and shatian pomelo can absorb endophyte of plant, connects
Kind effect is good.
(3) endophyte of plant that the present invention is inoculated with is by bacillus amyloliquefaciens, citric acid bacillus, the raw Erwinia of grass, production
Sour Klebsiella, Pseudomonas fluorescens composition, the enzyme activity of single bacillus amyloliquefaciens secretion is not high, to pathogen
Disease resistance is weak, using citric acid bacillus, the raw Erwinia of grass, acid-producing Klebsiella bacterium, Pseudomonas fluorescens and solution starch bud
Spore bacillus coordinates, and on the one hand strengthens absorbability of the endophyte of plant to nutriment in plant, endophyte of plant and pathogen
Competition, nutriment needed for growth of pathogenic bacteria lack and dead；Endophyte of plant coordinates at the same time, secretes suitable promotion Shatian
The growth hormone system of shaddock growth, auxin system include heteroauxin, the basic element of cell division, gibberellin etc.；Another aspect plant
Endophyte can secrete alkaloid, flavomycoin, azophenlyene carboxylic acid, Teng Hua pyos, pyrrolnitrin etc. in shatian pomelo plant body
Antibiotic and a variety of Antagonistic proteins, various plants endophyte, which forms the wide suppression antibacterial bacteriostatic of poisoning scope and carries, is, while Xie Dian
Sorodium spore bar coordinates with citric acid bacillus, catabolic enzyme, the cell membrane of degradative fungi can be secreted in shatian pomelo body, while also secrete
Enzyme needed for various plants growth, such as superoxide dismutase, add phenol oxidase, catalase, improves shatian pomelo
Resistance.
(4) bacillus amyloliquefaciens that use of the present invention be placed in containing ash arrhizus bacteria, wilt culture medium in tame,
The bacillus amyloliquefaciens selected have stronger sterilization functions, improve the disease resistance of shatian pomelo.
Embodiment
With reference to embodiment and experiment, the invention will be further described.
Embodiment 1
A kind of high-yield method of shatian pomelo：The inoculated plant endophyte into shatian pomelo plant；The inoculation method is：Choose
As base-material, the percent water for adjusting yellow mud is added and is made of konjak starch, sodium acrylate yellow mud for 25% into yellow mud
Water-retaining agent and endophyte of plant, stir evenly, then after the root of yellow mud is wrapped in the root of shatian pomelo plant, using wavelength
The visible ray of 520nm is cultivated two days with the intensity of illumination irradiation of 1100Lux, then is transplanted；The endophyte of plant is by viable count
Quantity ratio is 20:1：5:3:It is 10 bacillus amyloliquefaciens, citric acid bacillus, the raw Erwinia of grass, acid-producing Klebsiella bacterium, glimmering
Light pseudomonad forms；On the basis of yellow mud, the additive amount of the endophyte of plant is 5g/Kg.The solution starch gemma
Bacillus also carries out the antibacterial domestication of following steps:Bacillus amyloliquefaciens are placed in culture medium after cultivating 2d and added into culture medium
Add ash arrhizus bacteria, wilt, when continuing fermentation to the ash arrhizus bacteria in culture medium, wilt whole kill, culture medium
When total enzyme activity is not less than 832U/ml, picks out bacillus amyloliquefaciens and obtain the bacillus amyloliquefaciens；The training
Based formulas is supported as beef extract 6g/L, potato 13g/L, glucose 5g/L, sodium chloride 5g/L, ammonium chloride 5g/L.
Embodiment 2
A kind of high-yield method of shatian pomelo：The inoculated plant endophyte into shatian pomelo plant；The inoculation method is：Choose
As base-material, the percent water for adjusting yellow mud is added and is made of konjak starch, sodium acrylate yellow mud for 20% into yellow mud
Water-retaining agent and endophyte of plant, stir evenly, then after the root of yellow mud is wrapped in the root of shatian pomelo plant, using wavelength
The visible ray of 670nm is cultivated two days with the intensity of illumination irradiation of 800Lux, then is transplanted；The endophyte of plant is by viable count number
Amount is than being 20:4：3:6:9 bacillus amyloliquefaciens, citric acid bacillus, the raw Erwinia of grass, acid-producing Klebsiella bacterium, fluorescence
Pseudomonad forms；On the basis of yellow mud, the additive amount of the endophyte of plant is 15g/Kg.The solution starch gemma bar
Bacterium also carries out the antibacterial domestication of following steps:Bacillus amyloliquefaciens are placed in culture medium after cultivating 1d and are added into culture medium
Ash arrhizus bacteria, wilt, continue fermentation to the ash arrhizus bacteria in culture medium, wilt all kill when, culture medium it is total
When enzyme activity is not less than 832U/ml, picks out bacillus amyloliquefaciens and obtain the bacillus amyloliquefaciens；The culture
Based formulas is beef extract 6g/L, potato 13g/L, glucose 5g/L, sodium chloride 5g/L, ammonium chloride 5g/L.
Embodiment 3
A kind of high-yield method of shatian pomelo：The inoculated plant endophyte into shatian pomelo plant；The inoculation method is：Choose
As base-material, the percent water for adjusting yellow mud is added and is made of konjak starch, sodium acrylate yellow mud for 22% into yellow mud
Water-retaining agent and endophyte of plant, stir evenly, then after the root of yellow mud is wrapped in the root of shatian pomelo plant, using wavelength
The visible ray of 600nm is cultivated two days with the intensity of illumination irradiation of 1100Lux, then is transplanted；The endophyte of plant is by viable count
Quantity ratio is 20:3：4:5:It is 9 bacillus amyloliquefaciens, citric acid bacillus, the raw Erwinia of grass, acid-producing Klebsiella bacterium, glimmering
Light pseudomonad forms；On the basis of yellow mud, the additive amount of the endophyte of plant is 10g/Kg.The solution starch gemma
Bacillus also carries out the antibacterial domestication of following steps:Bacillus amyloliquefaciens are placed in culture medium after cultivating 2d and added into culture medium
Add ash arrhizus bacteria, wilt, when continuing fermentation to the ash arrhizus bacteria in culture medium, wilt whole kill, culture medium
When total enzyme activity is not less than 832U/ml, picks out bacillus amyloliquefaciens and obtain the bacillus amyloliquefaciens；The training
Based formulas is supported as beef extract 6g/L, potato 13g/L, glucose 5g/L, sodium chloride 5g/L, ammonium chloride 5g/L.
Comparative example 1
The shatian pomelo high-yield method of comparative example 1 is substantially the same manner as Example 1, and difference lies in comparative example 1 uses wavelength
The visible ray of 450nm is cultivated two days with the intensity of illumination irradiation of 100Lux, then is transplanted.
Comparative example 2
The shatian pomelo high-yield method of comparative example 2 is substantially the same manner as Example 1, and difference lies in comparative example 2 is only in plant
Raw bacterium is bacillus amyloliquefaciens.
Comparative example 3
The shatian pomelo high-yield method of comparative example 3 is substantially the same manner as Example 1, and difference lies in the solution starch that comparative example 3 uses
Bacillus be not disposed in containing ash arrhizus bacteria, wilt culture medium in tame.
Comparative example 4
The shatian pomelo high-yield method of comparative example 4 is substantially the same manner as Example 1, and difference lies in comparative example 4 uses plant endogenesis
Bacterium by wait viable count quantity than bacillus amyloliquefaciens, citric acid bacillus, the raw Erwinia of grass, acid-producing Klebsiella bacterium, glimmering
Light pseudomonad forms.
Comparative example 5
Comparative example 5 is blank comparative example, i.e., the shatian pomelo high-yield method of comparative example 5 is without artificial infection endophyte.
In order to illustrate the technique effect of the present invention, following experiment；
The shatian pomelo high-yield method provided according to 1~embodiment of embodiment 3 and 1~comparative example of comparative example 5 plants Shatian
Shaddock, when planting the 4th year, count shatian pomelo suffers from insect pest state of an illness condition, and per mu yield, and record is in table 1 below.
Table 1
As shown in Table 1, embodiment 1 is all high compared to the disease resistance and per mu yield of 1~comparative example of comparative example 5, comparative example 1
Yield and disease resistance it is poor compared to embodiment 1, illustrate using suitable illumination spoke, to contribute to the inoculation of endophyte of plant,
So as to promote the growth of shatian pomelo, the disease resistance and yield of shatian pomelo are improved, from 2~comparative example of comparative example 4, use
The thalline system of endophyte of plant is different, and different with disease resistance effect, the growing way of shatian pomelo is different, directly affects sand
The yield of field shaddock.
Described above is the detailed description for the present invention preferably possible embodiments, but embodiment is not limited to this hair
Bright patent claim, equal change or the modification change completed under the technical spirit suggested by all present invention, should all belong to
Cover the scope of the claims in the present invention.
Claims (6)
- A kind of 1. high-yield method of shatian pomelo, it is characterised in that the inoculated plant endophyte into shatian pomelo plant；The inoculation side Method is：Choose yellow mud and be used as base-material, adjust the percent water of yellow mud for 20%~25% add into yellow mud water-retaining agent with Endophyte of plant, stirs evenly, then after the root of yellow mud is wrapped in the root of shatian pomelo plant, using 520~670nm's of wavelength Radiation of visible light is cultivated two days, then is transplanted；The endophyte of plant is by bacillus amyloliquefaciens, citric acid bacillus, the raw Europe of grass Wen bacterium, acid-producing Klebsiella bacterium, Pseudomonas fluorescens composition；On the basis of yellow mud, the additive amount of the endophyte of plant For 5~15g/Kg.
- 2. the high-yield method of a kind of shatian pomelo according to claim 1, it is characterised in that the endophyte of plant is by living Bacterium number quantity ratio is 20:1~4：3~5:3~6:9~10 bacillus amyloliquefaciens, citric acid bacillus, the raw Erwinia of grass, Acid-producing Klebsiella bacterium, Pseudomonas fluorescens composition.
- A kind of 3. high-yield method of shatian pomelo according to claim 1, it is characterised in that the bacillus amyloliquefaciens Also carry out the antibacterial domestication of following steps:Bacillus amyloliquefaciens are placed in culture medium after cultivating 1~2d and added into culture medium Add ash arrhizus bacteria, wilt, when continuing fermentation to the ash arrhizus bacteria in culture medium, wilt whole kill, pick out solution Bacillus amyloliquefaciens are to obtain the bacillus amyloliquefaciens；The culture medium prescription is beef extract 6g/L, potato 13g/L, glucose 5g/L, sodium chloride 5g/L, ammonium chloride 5g/L.
- A kind of 4. high-yield method of shatian pomelo according to claim 3, it is characterised in that the grey mold in the culture medium When germ, wilt all kill, the total enzyme activity of culture medium is not less than 832U/ml.
- 5. the high-yield method of a kind of shatian pomelo according to claim 1, it is characterised in that the water-retaining agent is formed sediment by konjaku Powder, sodium acrylate composition.
- 6. the high-yield method of a kind of shatian pomelo according to claim 1, it is characterised in that the illumination of the visible ray is strong Spend for 800~1100Lux.
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