CN107926202A - A kind of method for promoting half abortive seed of lichee to sprout - Google Patents
A kind of method for promoting half abortive seed of lichee to sprout Download PDFInfo
- Publication number
- CN107926202A CN107926202A CN201711261179.6A CN201711261179A CN107926202A CN 107926202 A CN107926202 A CN 107926202A CN 201711261179 A CN201711261179 A CN 201711261179A CN 107926202 A CN107926202 A CN 107926202A
- Authority
- CN
- China
- Prior art keywords
- seed
- lichee
- abortive
- litchi chinensis
- fruit
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01C—PLANTING; SOWING; FERTILISING
- A01C1/00—Apparatus, or methods of use thereof, for testing or treating seed, roots, or the like, prior to sowing or planting
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Soil Sciences (AREA)
- Environmental Sciences (AREA)
- Pretreatment Of Seeds And Plants (AREA)
Abstract
The present invention provides a kind of method for promoting half abortive seed of lichee to sprout, and in particular to fruit breeding field.The method that half abortive seed of promotion lichee of the present invention is sprouted comprises the following steps：1) half abortion Litchi chinensis Seeds are soaked into 2~3d in water, obtains the sufficient Litchi chinensis Seeds of imbibition；2) vernalization is carried out after skin being planted at the sufficient Litchi chinensis Seeds cutting kind base of a fruit of the step 1) imbibition.Using the method for the present invention, the germination percentage of half abortive seed can bring up to more than 60% from 5%, significantly improve the germination percentage of half abortive seed of lichee, greatly speed up lichee breeding process.
Description
Technical field
The invention belongs to fruit breeding field, and in particular to a kind of method for promoting half abortive seed of lichee to sprout.
Background technology
Lichee is that south China originates in and local product fruits, is the most important economy in the provinces and regions such as Guangdong, Guangxi, Fujian, Hainan
One of fruit tree, there are breed structure is unreasonable and the problems such as maturity period high concentration, varieties distribution is to solve for lichee industry at present
One important measures of this two hang-up.It is badly in need of cultivating Early maturity and special late-maturing lichee new varieties in production, improves lichee production
The present situation of industry maturity period high concentration.Crossbreeding is to realize that Litchi Varieties innovate most important mode.Lichee crossbreeding consumes
When it is laborious, fruit is not easy very much, and seed is even more abnormal precious.Our research is found：Different lichee cross combinations obtain
Seed, its development degree (development completely, half abortion and abortive seed) is different, and the highest combination of seeds abortion rate is purple ma's happiness
× glutinous rice wrapped in lotus leaves is up to 43.28%, and the minimum combination of seeds abortion rate is that to increase control early black leaf × March is red be 6.54%, 40 combinations kind
The average abortion rate of son is up to 23.45%.
Full grown Litchi chinensis Seeds, take after planting in horse back soaking water, and after Seed imbibition is complete, plastic basket is put in taking-up into
In, wet gauze vernalization is covered, 2~3 days endogenous budding rates of seed can be up to more than 90%；For the Litchi chinensis Seeds of half abortion, such as
Fruit takes same method to handle, and seed germination rate is low, and less than 5%, Sprouting rate is also slow.Sent out in lichee crossbreeding practice
Existing, female parent is the combination of Early maturity and early-mature litchi kind, and half abortive seed accounting is big, as March is red and half abortion kind of cv. Feizixiao
Sub- ratio is up to more than 90%.Therefore, there is an urgent need to invent a kind of method for being efficiently promoting the sprouting of half abortive seed of lichee, carry
The germination percentage of high hybrid seed, accelerates the process of lichee Early maturity breeding.
The content of the invention
In view of this, it is an object of the invention to provide it is a kind of can efficiently promote half abortion Litchi chinensis Seeds sprout method,
It is easy to operate, efficient.
In order to realize foregoing invention purpose, the present invention provides following technical scheme：
The present invention provides a kind of method for promoting half abortive seed of lichee to sprout, comprise the following steps：
1) half abortion Litchi chinensis Seeds are soaked into 2~3d in water, obtains the sufficient Litchi chinensis Seeds of imbibition；
2) vernalization is carried out after skin being planted at the sufficient Litchi chinensis Seeds cutting kind base of a fruit of the step 1) imbibition；
Preferably, the condition of the step 1) immersion is：27~33 DEG C of temperature.
Preferably, the step 1) immersion process, water did not had 2~3cm of seed.
Preferably, the condition of step 2) presprouting of seeds is：27 DEG C~33 DEG C of temperature, humidity 60~70%.
Preferably, the position cut described in step 2) is embryo growing point both sides at kind of the base of a fruit.
Preferably, described be cut into forms splay notch in embryo growing point both sides.
Preferably, the depth of the cutting is 0.8~1.3mm, and length is 5~10mm.
The present invention provides a kind of method for promoting lichee half abortive seed to sprout, by being cut to Litchi chinensis Seeds,
After solving the immersion of half abortion Litchi chinensis Seeds, the problem of bud ratio is low, and Sprouting rate is slow, improve the germinations of half abortion Litchi chinensis Seeds
Rate, accelerates the process of lichee crossbreeding；Put into practice according to the vernalization of Litchi Varieties " cv. Feizixiao " and " March is red ", utilize the present invention
Method, the germination rate of half abortive seed improves 10 times or so.
Brief description of the drawings
Fig. 1 is half abortive seed germination shape.
Embodiment
The present invention provides a kind of method for promoting half abortive seed of lichee to sprout, comprise the following steps：
1) half abortion Litchi chinensis Seeds are soaked into 2~3d in water, obtains the sufficient Litchi chinensis Seeds of imbibition；
2) vernalization is carried out after skin being planted at the sufficient Litchi chinensis Seeds cutting kind base of a fruit of the step 1) imbibition；
In the present invention, half abortion Litchi chinensis Seeds are soaked into 2~3d in water, obtains the sufficient Litchi chinensis Seeds of imbibition.Institute
It is preferably the fresh seeds obtained then after mid-June to early July picking fruit to state seed；Preferably, stood after harvesting lichee
Pulp is rejected, is cleaned up with clear water, obtains the seed.Half abortive seed be preferably grain it is smaller, kind skin fold wrinkle
Tarnish, embryo vigor is normal, and cotyledon development is incomplete, only accounts for the 1/2~1/3 of kind of chamber, storage nutrient is inadequate.In the present invention
In, the immersion is preferably to be placed in seed in the device of collet to soak；Described device is preferably vinyl disc or bucket；The leaching
The amount of seed accounts for the 2/3 of vinyl disc or bucket volume during bubble, pours into tap water；The amount of the tap water there preferably was not seed, more
Preferably do not had and be higher by 2~3cm；The immersion is preferably soaked at normal temperatures, and the room temperature is preferably specially 27~33 DEG C,
More preferably 30 DEG C；Water is preferably changed during the immersion daily, it is preferred respectively to change a water sooner or later daily；The immersion when
Between be preferably 2~3d, more preferably soak 2d.
In the present invention, the vernalization preferably carries out in plastic basket, and the specification of the plastic basket is preferably grown
× wide × a height of 30cm × 20cm × 10cm.In the present invention, it is preferably 27 to the temperature of the half abortive seed vernalization~
33 DEG C, more preferably 30 DEG C；The relative air humidity of the vernalization is preferably 60~70%, in order to keep relative humidity
Stability, preferable in of the invention that gauze is also covered on seed, the gauze preferably needs moisturizing of sprinkling water, and the watering is excellent
Choosing is respectively sprinkled water once sooner or later daily, and gauze, which is soaked but do not flowed out, with water is advisable.
In the present invention, half abortive seed is cut and carries out vernalization after planting skin at kind of the base of a fruit, the position of the cutting is preferred
For embryo growing point both sides at kind of the base of a fruit, preferred cut portion is kind of a skin, is partly the tissue at kind of the base of a fruit；It is excellent after the cutting
It is selected in embryo growing point both sides and forms splay notch；The depth of the cutting preferably only cuts and breaks in the seed coat and plant group at the base of a fruit
Knit, do not injure embryo, preferred depth is 0.8~1.3mm, and length is 5~10mm；The cutting preferably uses secateurs,
It is preferred to be cut using the upper cutter head of secateurs；Heretofore described half abortive seed, embryo be difficult it is prominent break in the seed coat, it is necessary to
Constraint of kind of the skin to kind of embryonic development is released by external force, embryo can be dashed forward and breaks in the seed coat, successfully sprouts, so as to greatly improve lichee
Hybrid seed germination percentage.
The method sprouted with reference to embodiment to promotion Litchi chinensis Seeds provided by the invention is described in detail, still
They cannot be interpreted as limiting the scope of the present invention.
Embodiment 1
5056, seed for gathering lichee " cv. Feizixiao " in 2015, after Litchi chinensis Seeds are soaked 2d in water, imbibition is complete,
Obtain 216 complete seeds of development, 4840 half abortive seeds.The embryo growing point both sides cutting eight at kind of the base of a fruit of half abortive seed
Vernalization is carried out after notched cut, the seed of germination is chosen daily, is sowed；Half abortive seed germinates 3095 altogether, germination
Rate is 63.94%.
Embodiment 2
2347, seed for gathering lichee " Fengshan red lantern " in 2015, after Litchi chinensis Seeds are soaked 2d in water, imbibition
Completely, 697 hairs are obtained and develop complete seed, 1650 half abortive seeds.By half abortive seed at kind of the base of a fruit embryo growing point
Vernalization is carried out after both sides cutting splayed notch, the seed of germination is chosen daily, is sowed.Half abortive seed is counted to send out altogether
Bud 1010, germination percentage 61.2%.
Embodiment 3
1400, seed for gathering lichee " osmanthus taste " in 2015, after Litchi chinensis Seeds are soaked 2d in water, imbibition is complete, obtains
To 860 complete seeds of development, 540 half abortive seeds.The embryo growing point both sides cutting eight at kind of the base of a fruit by half abortive seed
Vernalization is carried out after notched cut, the seed of germination is chosen daily, is sowed.Count half abortive seed to germinate altogether 336, send out
Bud rate is 62.3%.
Comparative example 1
Litchi chinensis Seeds are soaked 2d, imbibition is complete by 24232, the seed of collection artificial hybridization breeding lichee in 2014 in water
Obtain developing complete 20161, seed, half abortive seed 4071, direct vernalization after complete.Count half abortive seed and germinate 0 altogether
, germination percentage 0.
Comparative example 2
Litchi chinensis Seeds are soaked 2d, after imbibition is complete by 6362, seed for gathering lichee " cv. Feizixiao " in 2014 in water
Obtain developing complete 2952, seed, half abortive seed 3410, direct vernalization.Count half abortive seed to germinate altogether 229, send out
Bud rate is 6.74%.
As seen from the above embodiment, using the method for the present invention, half abortive seed germination percentage can be made to bring up to more than 60%,
It is remarkably improved the germination percentage of Litchi chinensis Seeds.
The above is only the preferred embodiment of the present invention, it is noted that for the ordinary skill people of the art
For member, various improvements and modifications may be made without departing from the principle of the present invention, these improvements and modifications also should
It is considered as protection scope of the present invention.
Claims (7)
1. a kind of method for promoting half abortive seed of lichee to sprout, comprises the following steps：
1) half abortion Litchi chinensis Seeds are soaked into 2~3d in water, obtains the sufficient Litchi chinensis Seeds of imbibition；
2) vernalization is carried out after skin being planted at the sufficient Litchi chinensis Seeds cutting kind base of a fruit of the step 1) imbibition.
2. according to the method described in claim 1, it is characterized in that, the condition of the step 1) immersion is：27~33 DEG C of temperature.
3. method according to claim 1 or 2, it is characterised in that the step 1) immersion process, water do not had seed 2~
3cm。
4. according to the method described in claim 1, it is characterized in that, the vernalization condition of step 2) half abortive seed is：Temperature
27 DEG C~33 DEG C of degree, humidity 60~70%.
5. according to the method described in claim 1, it is characterized in that, the position cut described in step 2) is embryo life at kind of the base of a fruit
Long point both sides.
6. method according to claim 1 or 5, it is characterised in that described be cut into forms eight in embryo growing point both sides
The notch of font.
7. according to the method described in claim 6, it is characterized in that, the depth of the cutting is 0.8~1.3mm, length for 5~
10mm。
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201711261179.6A CN107926202B (en) | 2017-12-04 | 2017-12-04 | Method for promoting germination of litchi semi-aborted seeds |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201711261179.6A CN107926202B (en) | 2017-12-04 | 2017-12-04 | Method for promoting germination of litchi semi-aborted seeds |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN107926202A true CN107926202A (en) | 2018-04-20 |
| CN107926202B CN107926202B (en) | 2020-10-30 |
Family
ID=61947481
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201711261179.6A Expired - Fee Related CN107926202B (en) | 2017-12-04 | 2017-12-04 | Method for promoting germination of litchi semi-aborted seeds |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN107926202B (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113935963A (en) * | 2021-10-08 | 2022-01-14 | 广东省农业科学院果树研究所 | Image recognition detection method and system for litchi embryo development degree |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20080026087A1 (en) * | 2003-06-24 | 2008-01-31 | Santiago Rull Prous | Litchi Sinensis Extracts Containing Oligomeric Proanthocyanidins and Processes of Making Thereof |
| CN104838760A (en) * | 2015-05-06 | 2015-08-19 | 合浦县公馆镇香山村马拉坡鸡嘴荔枝农民专业合作社 | Method for preventing cracking of chicken beak litchis |
| CN105028176A (en) * | 2015-06-10 | 2015-11-11 | 广东省农业科学院果树研究所 | Rapid seedling selection method of litchi |
| CN106879275A (en) * | 2017-01-10 | 2017-06-23 | 中国科学院华南植物园 | A kind of quick method for effectively improving Hainan Chinese scholartree percentage of seedgermination |
| CN106961879A (en) * | 2017-05-22 | 2017-07-21 | 广东省农业科学院果树研究所 | A kind of efficient lichee hybridization fruit manually takes kind of a method |
-
2017
- 2017-12-04 CN CN201711261179.6A patent/CN107926202B/en not_active Expired - Fee Related
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20080026087A1 (en) * | 2003-06-24 | 2008-01-31 | Santiago Rull Prous | Litchi Sinensis Extracts Containing Oligomeric Proanthocyanidins and Processes of Making Thereof |
| CN104838760A (en) * | 2015-05-06 | 2015-08-19 | 合浦县公馆镇香山村马拉坡鸡嘴荔枝农民专业合作社 | Method for preventing cracking of chicken beak litchis |
| CN105028176A (en) * | 2015-06-10 | 2015-11-11 | 广东省农业科学院果树研究所 | Rapid seedling selection method of litchi |
| CN106879275A (en) * | 2017-01-10 | 2017-06-23 | 中国科学院华南植物园 | A kind of quick method for effectively improving Hainan Chinese scholartree percentage of seedgermination |
| CN106961879A (en) * | 2017-05-22 | 2017-07-21 | 广东省农业科学院果树研究所 | A kind of efficient lichee hybridization fruit manually takes kind of a method |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113935963A (en) * | 2021-10-08 | 2022-01-14 | 广东省农业科学院果树研究所 | Image recognition detection method and system for litchi embryo development degree |
Also Published As
| Publication number | Publication date |
|---|---|
| CN107926202B (en) | 2020-10-30 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN105454047B (en) | A kind of tissue culture and rapid propagation method of Eucalyptus cloeziana | |
| CN103843548A (en) | Method used for quickly breaking dormancy of peony seed epicotyl | |
| CN106386478A (en) | Phoebe zhennan tissue culture rapid breeding method | |
| CN105379624B (en) | A kind of tissue culture and rapid propagation method of Eucalyptus pellita | |
| CN107258537A (en) | A kind of eucalyptus breeding tail eucalyptus camaldulensis DH191 4 tissue culture and rapid propagation method | |
| CN107027627A (en) | A kind of micro-tuber propagation method of David's-harp IMMATURE EMBRYOS CULTURE | |
| CN106818468A (en) | A kind of shellflower seed asepsis sprouting and rapid propagation method | |
| CN106417014A (en) | Method for breeding and cultivating Paphiopedilum macranthum | |
| CN106172011B (en) | A kind of dwarf banana high temperature light culture tissue culture and rapid propagation method | |
| CN105900837B (en) | A kind of method for cultivating fast breeding iron orchid species seedling by suspending | |
| CN107852886A (en) | A kind of method for promoting swordleaf dragon tree seed to sprout | |
| CN107926202A (en) | A kind of method for promoting half abortive seed of lichee to sprout | |
| CN105340675B (en) | A kind of vinegrowing fruiting management method | |
| CN104429486A (en) | Method for culturing immature seeds of processed tomatoes into seedlings | |
| CN105359905A (en) | Caryaillinoensis high pressure seedling growing method | |
| CN109197196A (en) | A kind of method of pachydermia netted melon cuttage and seedling culture | |
| CN1181723C (en) | Sexual propagation method for wilsonii | |
| CN105638188B (en) | A kind of method for culturing seedlings of new pteris fern | |
| CN104054578B (en) | The tissue culture and rapid propagation method of Dian clove | |
| CN107047205A (en) | One kind promotes horse-tail lateral sprouting method | |
| CN107182355A (en) | A kind of Malus sieversii seed accelerating germination method | |
| CN108633379B (en) | Method for breaking dormancy of cercis gigantea seeds | |
| CN106922523B (en) | A method of induction Chinese chestnut polyploid is impregnated based on plumule | |
| CN110326537A (en) | A kind of Chinese toon inducing clumping bud and enrichment procedure | |
| CN110463607A (en) | The method of wild Europe Lee's leaf tissue culture |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20201030 Termination date: 20211204 |
|
| CF01 | Termination of patent right due to non-payment of annual fee |