CN107892723A - The processing method of protein content in a kind of reduction tamarind seed polysaccharide - Google Patents
The processing method of protein content in a kind of reduction tamarind seed polysaccharide Download PDFInfo
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- CN107892723A CN107892723A CN201711291712.3A CN201711291712A CN107892723A CN 107892723 A CN107892723 A CN 107892723A CN 201711291712 A CN201711291712 A CN 201711291712A CN 107892723 A CN107892723 A CN 107892723A
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- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08B—POLYSACCHARIDES; DERIVATIVES THEREOF
- C08B37/00—Preparation of polysaccharides not provided for in groups C08B1/00 - C08B35/00; Derivatives thereof
- C08B37/0003—General processes for their isolation or fractionation, e.g. purification or extraction from biomass
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Abstract
The invention discloses a kind of processing method for reducing protein content in tamarind seed polysaccharide, comprise the following steps：Tamarind seed polysaccharide extract solution is subjected to acid adjustment processing using the citric acid solution of high concentration, pH to 3.0 4.0 is adjusted, then stands at room temperature and centrifugation obtains supernatant I, supernatant I is concentrated into certain volume, obtains supernatant concentrate；Supernatant concentrate is placed under 50 DEG C of environment and freezed, is taken out after freezing completely, is then melted at room temperature, then centrifuge, be repeated 1 times freezing, melt and the supernatant II after freeze thawing is obtained after centrifuging；Ethanol is slowly added into supernatant II and is stirred, ethanol final concentration is reached 40 60%, alcohol precipitation is carried out, is then centrifuged for obtaining sediment I；Sediment I is crushed, is added in ethanol solution, is sufficiently stirred washing repeatedly, is then centrifuged for obtaining the sediment II after ethanol solution washing；Add organic reagent into sediment II to carry out dewater treatment and air-dry, the solids obtained after air-drying is crushed, and obtains tamarind seed polysaccharide.
Description
Technical field
The invention belongs to field of plant extraction, and in particular to a kind of method for reducing protein content in tamarind seed polysaccharide.
Background technology
Tamarind seed polysaccharide is a kind of edible neutral polysaccharide, compared with other edible polysaccharide, except with stable, breast
Change, thickening, outside water conservation and film forming function, also there is unique physicochemical property, such as good heat-resisting, salt tolerant, acidproof, freeze-resistant and
Defrosting etc., play the role of in the food industry irreplaceable.Abroad, tamarind seed polysaccharide research and application it is existing very long
For a period of time, the industrialized production of the Japanese tamarind seed polysaccharide that takes the lead in realizing, and being sold as food additives.Utilize its increasing
Consistence, gelation, stability and emulsibility, tamarind seed polysaccharide are widely used in ice cream, ice cream, tartar sauce, cooking product, drink
In the production of material etc..It is relevant to report less, related technology because development and application of the China to tamarind seed polysaccharide is started late
Also immature with standard, far behind the U.S. and Japan and other countries, most of tamarind seed polysaccharide product all rely on import.
The market demand of China's tamarind seed polysaccharide is big, has a extensive future.2015, China's issue tamarind seed polysaccharide glue was made
National standard (GB1886.106-2015) for food additives is, it is specified that protein content must not be high in tamarind seed polysaccharide product
In 3%.At present, the product that the extraction of the traditional extraction technique of tamarind seed polysaccharide, such as hot water, organic acid extraction obtain, it is difficult
It is up to state standards.Conventional chemical reagent method for removing protein, can be to more as the methods of Sevage reagents, trichloroacetic acid is handled
Sugared structure produces destruction in itself, meanwhile, it will also reduce product yield, improve production cost etc., thus be unsuitable for carrying out greatly
The production of scale, so needing a kind of tamarind seed polysaccharide of improvement to take off protein Process.
The content of the invention
The present invention is, and it is an object of the present invention to provide a kind of reduce albumen in tamarind seed polysaccharide in order to solving the above problems and carry out
The method of matter content.
The invention provides a kind of processing method for reducing protein content in tamarind seed polysaccharide, tamarind seed polysaccharide is extracted
Liquid carries out reducing the tamarind seed polysaccharide after protein content is handled and handled, and has the feature that, comprises the following steps：
Step 1, the tamarind seed polysaccharide extract solution is subjected to acid adjustment processing using the citric acid solution of high concentration, adjusts pH
To 3.0-4.0, then stand at room temperature and centrifugation obtains supernatant I, the supernatant I is concentrated into certain volume, obtained
Clear concentrate；
Step 2, the supernatant concentrate is placed under -50 DEG C of environment and freezed, taken out after freezing completely, then melt at room temperature
Solution, then centrifuges, is repeated 1 times freezing, melts and the supernatant II after freeze thawing is obtained after centrifuging；
Step 3, it is slowly added to ethanol into the supernatant II and stirs, ethanol final concentration is reached 40-60%, carries out
Alcohol precipitation, it is then centrifuged for obtaining sediment I；
Step 4, the sediment is crushed, adds ethanol solution, be sufficiently stirred washing repeatedly, be then centrifuged for obtaining ethanol
Sediment II after solution washing；
Step 5, organic reagent is added into the sediment II to carry out dewater treatment and air-dry, what is obtained after air-drying consolidates
Body thing is crushed, and obtains the tamarind seed polysaccharide.
In reduction tamarind seed polysaccharide provided by the invention in the method for protein content, can also have such special
Sign：Wherein, the time of the alcohol precipitation in step 3 is 2h.
In reduction tamarind seed polysaccharide provided by the invention in the method for protein content, can also have such special
Sign：Wherein, the concentration of the ethanol solution in step 4 is 40-60%.
In reduction tamarind seed polysaccharide provided by the invention in the method for protein content, can also have such special
Sign：Wherein, the number of the agitator treating in step 4 is 1-2 times.
The effect of invention and effect
According to the method for reducing protein content in tamarind seed polysaccharide involved in the present invention, because employing isoelectric point knot
Close freeze-thaw method and ethanol washes twice, so the protein content for the tamarind seed polysaccharide being prepared is less than 3%, meet national mark
Tamarind seed polysaccharide accurate and that white is tasteless.Edible add is not suitable for it in addition, not used during the protein removal of the present invention
Add agent, obtained tamarind seed polysaccharide more meets food safety requirements.
Generally speaking, the tamarind seed polysaccharide being prepared using the method for the present invention is higher than national standard, and meets food
The requirement of additive, laid the foundation for the utilization of China's tamarind seed polysaccharide.
Brief description of the drawings
Fig. 1 is the flow chart that protein content in tamarind seed polysaccharide is reduced in embodiments of the invention.
Embodiment
In order that the technological means that the present invention realizes is easy to understand with effect, with reference to embodiments and accompanying drawing is to this
Invention is specifically addressed.
Embodiment one：Isoelectric precipitation removes protein method
Step 1,500mL tamarind seed polysaccharide extract solutions are measured, acid adjustment processing are carried out with the citric acid solution of high concentration, by pH
To 4.0, then centrifugation obtains supernatant I after standing 1h at room temperature, and supernatant I is concentrated in vacuo into the 1/2 of original volume, obtained for regulation
To supernatant concentrate.
Step 2, ethanol is slowly added into supernatant concentrate and is stirred, to ethanol final concentration of 50%, it is a large amount of heavy to occur
Form sediment, 2h is stood under low temperature, centrifuge, obtain sediment.
Step 3, isopropanol is added into sediment to carry out dewater treatment and air-dry, the solids obtained after air-drying is carried out
Crush, obtain tamarind seed polysaccharide A.
Tamarind seed polysaccharide A obtained by the present embodiment is white powder, tasteless, and protein content is measured by Kjeldahl's method
For 6.7%, higher than the 3% of national Specification.
Embodiment two：Isoelectric point combination freeze-thaw method removes the protein in tamarind seed polysaccharide
Step 1,500mL tamarind seed polysaccharide extract solutions are measured, acid adjustment processing are carried out with the citric acid solution of high concentration, by pH
To 3.5, then centrifugation obtains supernatant I after standing 1h at room temperature, and supernatant I is concentrated in vacuo into the 1/2 of original volume, obtained for regulation
To supernatant concentrate.
Step 2, supernatant concentrate is placed in -50 DEG C of environment and freezed, taken out after freezing completely, then melt at room temperature,
Then centrifuge, be repeated 1 times freezing, melt and the supernatant II after freeze thawing is obtained after centrifuging.
Step 3, ethanol is slowly added into supernatant II and is stirred, ethanol final concentration is reached 60%, then under low temperature
Centrifugation obtains sediment after alcohol precipitation 2h.
Step 4, isopropanol is added into sediment to carry out dewater treatment and air-dry, the solids obtained after air-drying is carried out
Crush, obtain tamarind seed polysaccharide B.
Tamarind seed polysaccharide B obtained by the present embodiment is white powder, tasteless, measures protein by Kjeldahl's method and contains
Amount, it is 3.5%, higher than the 3% of national Specification.
Fig. 1 is the flow chart that protein content in tamarind seed polysaccharide is reduced in embodiments of the invention.
Embodiment three：As shown in figure 1, electricity point removes the protein in tamarind seed polysaccharide with reference to freeze-thaw method and ethanol washing
Step 1,500mL tamarind seed polysaccharide extract solutions are measured, acid adjustment processing are carried out with the citric acid solution of high concentration, by pH
To 3.1, then centrifugation obtains supernatant I after standing 1h at room temperature, and supernatant I is concentrated in vacuo into the 1/2 of original volume, obtained for regulation
To supernatant concentrate.
Step 2, supernatant concentrate is placed in -50 DEG C of environment and freezed, taken out after freezing completely, then melt at room temperature,
Then centrifuge, be repeated 1 times freezing, melt and the supernatant II after freeze thawing is obtained after centrifuging.
Step 3, ethanol is slowly added into supernatant II and is stirred, ethanol final concentration is reached 60%, then under low temperature
Centrifugation obtains sediment I after alcohol precipitation 2h.
Step 4, sediment I is crushed, adds the ethanol solution that 100mL concentration is 50%, stood after being sufficiently stirred washing
1h, it is then centrifuged for obtaining the sediment II after ethanol solution washs 1 time.
Step 5, isopropanol is added into sediment II to carry out dewater treatment and air-dry, the solids obtained after air-drying enters
Row crushes, and obtains tamarind seed polysaccharide C.
Tamarind seed polysaccharide C obtained by the present embodiment is white powder, tasteless, and protein content is measured by Kjeldahl's method
For 2.8%, meet national Specification is less than 3%.
Example IV：Isoelectric point combination freeze-thaw method and ethanol washing remove the protein in tamarind seed polysaccharide
Step 1,500mL tamarind seed polysaccharide extract solutions are measured, acid adjustment processing are carried out with the citric acid solution of high concentration, by pH
To 3.3, then centrifugation obtains supernatant I after standing 1h at room temperature, and supernatant I is concentrated in vacuo into the 1/2 of original volume, obtained for regulation
To supernatant concentrate.
Step 2, supernatant concentrate is placed in -50 DEG C of environment and freezed, taken out after freezing completely, then melt at room temperature,
Then centrifuge, be repeated 1 times freezing, melt and the supernatant II after freeze thawing is obtained after centrifuging.
Step 3, ethanol is slowly added into supernatant II and is stirred, ethanol final concentration is reached 40%, then under low temperature
Centrifugation obtains sediment I after alcohol precipitation 2h.
Step 4, sediment I is crushed, adds the ethanol solution that 100mL concentration is 40%, stood after being sufficiently stirred washing
1h, it is then centrifuged for obtaining sediment, the sediment is continued to smash to pieces, adds the ethanol solution that 100mL concentration is 40%, fully stir
1h is stood after mixing washing, is then centrifuged for the sediment after being washed twice II.
Step 5, isopropanol is added into the sediment II obtained after washing twice to carry out dewater treatment and air-dry, and will be air-dried
The solids obtained afterwards is crushed, and obtains tamarind seed polysaccharide D.
Tamarind seed polysaccharide D obtained by the present embodiment is white powder, and tasteless, measuring protein content by Kjeldahl's method is
1.1%, meet national Specification is less than 3%.
Embodiment five：Isoelectric point combination freeze-thaw method and ethanol washing remove the protein in tamarind seed polysaccharide
Step 1, measure 500mL tamarind seed polysaccharides extract solution and carry out acid adjustment processing using the citric acid solution of high concentration, will
PH is adjusted to 3.3, after then standing 1h at room temperature centrifugation obtain supernatant I, supernatant I is concentrated in vacuo to the 1/2 of original volume,
Obtain supernatant concentrate.
Step 2, supernatant concentrate is placed in -50 DEG C of environment and freezed, taken out after freezing completely, then melt at room temperature,
Then centrifuge, be repeated 1 times freezing, melt and the supernatant II after freeze thawing is obtained after centrifuging.
Step 3, ethanol is slowly added into supernatant II and is stirred, ethanol final concentration is reached 50%, then under low temperature
Centrifugation obtains sediment I after alcohol precipitation 2h.
Step 4, sediment I is smashed to pieces, adds the ethanol solution that 100mL concentration is 50%, stood after being sufficiently stirred washing
1h, it is then centrifuged for obtaining sediment, the sediment is continued to smash to pieces, adds the ethanol solution that 100mL concentration is 50%, fully stir
1h is stood after mixing washing, is then centrifuged for the sediment after being washed twice II.
Step 5, isopropanol is added into the sediment II obtained after washing twice to carry out dewater treatment and air-dry, and will be air-dried
The solids obtained afterwards is crushed, and obtains tamarind seed polysaccharide E.
Tamarind seed polysaccharide E obtained by the present embodiment is white powder, tasteless, and protein content is measured by Kjeldahl's method
For 1.2%, meet national Specification is less than 3%.
The effect of embodiment and effect
It can be seen from embodiment one, when removing protein method using isoelectric precipitation, the tamarind seed polysaccharide being prepared is
The tasteless powder of white and protein content are 6.7% higher than the 3% of national Specification.
It can be seen from embodiment two, when isoelectric point combination freeze-thaw method removes the protein in tamarind seed polysaccharide, it is prepared into
To tamarind seed polysaccharide be the tasteless powder of white and protein content is 3.5%, higher than the 3% of national Specification, show to adopt
It can reduce protein content with during freeze-thaw method.
It can be seen from embodiment three, the egg in using isoelectric point combination freeze-thaw method and ethanol washing removal tamarind seed polysaccharide
During white matter, the tamarind seed polysaccharide being prepared is the tasteless powder of white and protein content is 2.8%, less than national Specification
3%, show to reduce protein content when washing using freeze-thaw method and ethanol simultaneously and meet national standard.
It can be seen from example IV, the egg in using isoelectric point combination freeze-thaw method and ethanol washing removal tamarind seed polysaccharide
During white matter, the tamarind seed polysaccharide being prepared is the tasteless powder of white and protein content is 1.1%, less than national Specification
3%, show that protein content can be made lower and meet national standard when washing twice using freeze-thaw method and ethanol simultaneously.
It can be seen from embodiment five, removed when being washed twice using isoelectric point combination freeze-thaw method and ethanol in tamarind seed polysaccharide
Protein when, the tamarind seed polysaccharide that is prepared is the tasteless powder of white and protein content is 1.2%, less than national standard
Defined 3%, show that protein content can be made lower and meet national standard when washing twice using freeze-thaw method and ethanol simultaneously.
The processing method of protein content can in reduction tamarind seed polysaccharide according to involved by embodiment one to embodiment five
Know, when the protein in using isoelectric point combination freeze-thaw method and ethanol washing removal tamarind seed polysaccharide, the Luo Wang that is prepared
Sub- polysaccharide is less than 3% for the tasteless powder of white and protein content, meets national standard.Further, when utilization ethanol washing two
When secondary, the tamarind seed polysaccharide being prepared is the tasteless powder of white and protein content is lower.Therefore, using using isoelectric point knot
Conjunction freeze-thaw method and ethanol wash twice can just ensure to obtain protein content less than national standard and white tasteless sieve afterwards
Shop sign in the form of a streamer polysaccharide.
Above-mentioned embodiment is the preferred case of the present invention, is not intended to limit protection scope of the present invention.
In above-described embodiment one to five, supernatant I is concentrated into the 1/2 of original volume, and the organic reagent of use is isopropyl
Alcohol, but supernatant I can also be concentrated into the scope between the 1/3 or 1/3 to 1/2 of original volume in the present invention, and organic reagent is also
It can be the volatile reagent such as ether.
Claims (5)
1. a kind of processing method for reducing protein content in tamarind seed polysaccharide, reduction albumen is carried out to tamarind seed polysaccharide extract solution
Matter content handle and handled after tamarind seed polysaccharide, it is characterised in that comprise the following steps：
Step 1, by the tamarind seed polysaccharide extract solution using high concentration citric acid solution carry out acid adjustment processing, regulation pH to
3.0-4.0, then stand at room temperature and centrifugation obtains supernatant I, the supernatant I is concentrated into certain volume, obtains supernatant
Concentrate；
Step 2, the supernatant concentrate is placed under -50 DEG C of environment and freezed, taken out after freezing completely, then melt at room temperature,
Then centrifuge, be repeated 1 times freezing, melt and the supernatant II after freeze thawing is obtained after centrifuging；
Step 3, it is slowly added to ethanol into the supernatant II and stirs, ethanol final concentration is reached 40-60%, carries out alcohol
It is heavy, it is then centrifuged for obtaining sediment I；
Step 4, the sediment I is crushed, adds ethanol solution, it is multiple to be sufficiently stirred washing, is then centrifuged for obtaining ethanol molten
Sediment II after liquid washing；
Step 5, organic reagent is added into the sediment II to carry out dewater treatment and air-dry, the solids obtained after air-drying
Crushed, obtain the tamarind seed polysaccharide.
2. the processing method according to claim 1 for reducing protein content in tamarind seed polysaccharide, it is characterised in that：
Wherein, the scope of the above-mentioned certain volume in the step 1 is 1/3 to the 1/2 of the volume of supernatant I.
3. the processing method according to claim 1 for reducing protein content in tamarind seed polysaccharide, it is characterised in that：
Wherein, the time of the alcohol precipitation in the step 3 is 2h.
4. the processing method according to claim 1 for reducing protein content in tamarind seed polysaccharide, it is characterised in that：
Wherein, the concentration of the ethanol solution in the step 4 is 40-60%.
5. the processing method according to claim 1 for reducing protein content in tamarind seed polysaccharide, it is characterised in that：
Wherein, the number of the agitator treating in the step 4 is 1-2 times.
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| CN114181329A (en) * | 2021-12-27 | 2022-03-15 | 青岛琛蓝海洋生物工程有限公司 | Method for extracting xyloglucan in tamarind seeds |
| CN114304451A (en) * | 2021-12-22 | 2022-04-12 | 云南猫哆哩集团食品有限责任公司 | Tamarind fruit juice added with tamarind seed polysaccharide glue solution and preparation method thereof |
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| CN114304451A (en) * | 2021-12-22 | 2022-04-12 | 云南猫哆哩集团食品有限责任公司 | Tamarind fruit juice added with tamarind seed polysaccharide glue solution and preparation method thereof |
| CN114181329A (en) * | 2021-12-27 | 2022-03-15 | 青岛琛蓝海洋生物工程有限公司 | Method for extracting xyloglucan in tamarind seeds |
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