CN110511264A - A kind of extracting method of tamarind protein - Google Patents
A kind of extracting method of tamarind protein Download PDFInfo
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- CN110511264A CN110511264A CN201910870852.9A CN201910870852A CN110511264A CN 110511264 A CN110511264 A CN 110511264A CN 201910870852 A CN201910870852 A CN 201910870852A CN 110511264 A CN110511264 A CN 110511264A
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- 102000004169 proteins and genes Human genes 0.000 title claims abstract description 47
- 108090000623 proteins and genes Proteins 0.000 title claims abstract description 47
- 235000004298 Tamarindus indica Nutrition 0.000 title claims abstract description 44
- 238000000034 method Methods 0.000 title claims abstract description 32
- 240000004584 Tamarindus indica Species 0.000 title 1
- 230000001376 precipitating effect Effects 0.000 claims abstract description 60
- 241000596504 Tamarindus Species 0.000 claims abstract description 43
- 238000005119 centrifugation Methods 0.000 claims abstract description 34
- KRKNYBCHXYNGOX-UHFFFAOYSA-N citric acid Chemical compound OC(=O)CC(O)(C(O)=O)CC(O)=O KRKNYBCHXYNGOX-UHFFFAOYSA-N 0.000 claims abstract description 27
- 239000000463 material Substances 0.000 claims abstract description 26
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims abstract description 25
- 150000004676 glycans Chemical class 0.000 claims abstract description 25
- 229920001282 polysaccharide Polymers 0.000 claims abstract description 25
- 239000005017 polysaccharide Substances 0.000 claims abstract description 25
- 239000000843 powder Substances 0.000 claims abstract description 18
- 102000006395 Globulins Human genes 0.000 claims abstract description 15
- 108010044091 Globulins Proteins 0.000 claims abstract description 15
- 239000007853 buffer solution Substances 0.000 claims abstract description 13
- 239000003513 alkali Substances 0.000 claims abstract description 12
- 238000002525 ultrasonication Methods 0.000 claims abstract description 11
- 238000000605 extraction Methods 0.000 claims abstract description 10
- 150000003839 salts Chemical class 0.000 claims abstract description 9
- 239000013049 sediment Substances 0.000 claims abstract description 9
- QKNYBSVHEMOAJP-UHFFFAOYSA-N 2-amino-2-(hydroxymethyl)propane-1,3-diol;hydron;chloride Chemical compound Cl.OCC(N)(CO)CO QKNYBSVHEMOAJP-UHFFFAOYSA-N 0.000 claims abstract description 8
- 108010068370 Glutens Proteins 0.000 claims abstract description 7
- 102000009027 Albumins Human genes 0.000 claims abstract description 6
- 108010088751 Albumins Proteins 0.000 claims abstract description 6
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 claims description 42
- 239000000243 solution Substances 0.000 claims description 39
- 238000003756 stirring Methods 0.000 claims description 30
- 239000006228 supernatant Substances 0.000 claims description 28
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 claims description 22
- 239000007788 liquid Substances 0.000 claims description 20
- 238000002156 mixing Methods 0.000 claims description 20
- 239000011780 sodium chloride Substances 0.000 claims description 11
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 8
- 238000005406 washing Methods 0.000 claims description 5
- 238000007873 sieving Methods 0.000 claims description 4
- 235000013305 food Nutrition 0.000 abstract description 12
- 108010082495 Dietary Plant Proteins Proteins 0.000 abstract description 2
- 238000005516 engineering process Methods 0.000 abstract description 2
- 238000004519 manufacturing process Methods 0.000 abstract description 2
- VEXZGXHMUGYJMC-UHFFFAOYSA-N Hydrochloric acid Chemical compound Cl VEXZGXHMUGYJMC-UHFFFAOYSA-N 0.000 description 8
- 239000000284 extract Substances 0.000 description 6
- 238000005187 foaming Methods 0.000 description 5
- 238000011084 recovery Methods 0.000 description 5
- 239000003153 chemical reaction reagent Substances 0.000 description 3
- 230000008014 freezing Effects 0.000 description 3
- 238000007710 freezing Methods 0.000 description 3
- 238000010438 heat treatment Methods 0.000 description 3
- 230000000052 comparative effect Effects 0.000 description 2
- 239000012141 concentrate Substances 0.000 description 2
- 238000001514 detection method Methods 0.000 description 2
- 238000000502 dialysis Methods 0.000 description 2
- 239000006260 foam Substances 0.000 description 2
- 238000000751 protein extraction Methods 0.000 description 2
- 230000006920 protein precipitation Effects 0.000 description 2
- 239000002994 raw material Substances 0.000 description 2
- 238000000926 separation method Methods 0.000 description 2
- 108091006629 SLC13A2 Proteins 0.000 description 1
- 238000003916 acid precipitation Methods 0.000 description 1
- BFNBIHQBYMNNAN-UHFFFAOYSA-N ammonium sulfate Chemical compound N.N.OS(O)(=O)=O BFNBIHQBYMNNAN-UHFFFAOYSA-N 0.000 description 1
- 229910052921 ammonium sulfate Inorganic materials 0.000 description 1
- 235000011130 ammonium sulphate Nutrition 0.000 description 1
- 238000004458 analytical method Methods 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 238000005238 degreasing Methods 0.000 description 1
- 238000004090 dissolution Methods 0.000 description 1
- 239000012153 distilled water Substances 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 238000005121 nitriding Methods 0.000 description 1
- 235000019624 protein content Nutrition 0.000 description 1
- 239000012047 saturated solution Substances 0.000 description 1
- 239000007787 solid Substances 0.000 description 1
- 239000000725 suspension Substances 0.000 description 1
- 210000004885 white matter Anatomy 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/14—Extraction; Separation; Purification
- C07K1/145—Extraction; Separation; Purification by extraction or solubilisation
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/14—Extraction; Separation; Purification
- C07K1/30—Extraction; Separation; Purification by precipitation
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- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- General Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Biochemistry (AREA)
- Biophysics (AREA)
- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Medicinal Chemistry (AREA)
- Molecular Biology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Analytical Chemistry (AREA)
- Peptides Or Proteins (AREA)
Abstract
A kind of extracting method of tamarind protein, belongs to vegetable protein extractive technique field, food processing technology field.In order to solve the problems, such as that since there are viscosity height to cause extraction rate of protein low for polysaccharide in tamarind kernel, the present invention provides a kind of extracting methods of tamarind protein, and the tamarind kernel after extraction polysaccharide is crushed and obtains kernel powder；After the further Polysaccharide removing residual of citric acid, precipitating is collected by centrifugation；Precipitating is mixed with buffer solution Tris-HCl, adjusting pH is 11.0~13.0, carries out ultrasonication after the mixed material of acquisition is heated；Sediment is collected by centrifugation under the conditions of pH4.0-5.0, then obtains kernel alkali solubility globulin, kernel albumin, kernel salt dissolubility globulin, kernel alcohol soluble protein and kernel glutelin through step by step arithmetic.The present invention can be used for the production tamarind protein of scale.
Description
Technical field
The invention belongs to vegetable protein extractive technique field, food processing technology fields, and in particular to a kind of tamarind kind
The extracting method of benevolence albumen.
Background technique
The method that the current relatively conventional protein in tamarind kernel extracts is alkali extraction-acid precipitation, but is not had
Specific protein settles isoelectric point, and recovery rate is not high；Other uncommon extracting methods make egg by sodium chloride solution
White matter is separated by Micellization process wherein, tamarind kernel powder and the NaCl solution of degreasing is sufficiently mixed, then pass through salt
Analysis dialysis finally obtains protein concentrate, but protein extracting ratio is not high, and without tool that clear tamarind protein is included
Body protein type and content.After factory extracts tamarind kernel polysaccharide at present, there are also polysaccharide residuals to lead to the sticky influence kind of solution
The extraction of benevolence albumen causes valuable protein resource in tamarind kernel not utilize well.
Summary of the invention
In order to solve the problems, such as that extraction rate of protein is low in tamarind kernel, and clear tamarind protein type and contain
Amount, the present invention provides a kind of extracting methods of tamarind protein, include the following steps:
Step 1: the tamarind kernel after extraction polysaccharide is crushed, sieving, obtains kernel powder；
Step 2: by kernel powder and pH3.6 citric acid solution according to 1g:(30-50) mL ratio mixing, 800-1000
Turn/min stirring 30-50min, centrifugation is precipitated；
Step 3: the precipitating obtained in step 2 is mixed with Tris-HCl buffer solution, the precipitating and buffer solution
The ratio of Tris-HCl mixing is 1g:(20-40) mL, adjusting pH value is 11.0~13.0, obtains mixed material；
Step 4: mixed material heats 40-90min under the conditions of 60~70 DEG C；
Step 5: and then mixed material is subjected to ultrasonication, the treatment conditions of the ultrasonic wave are as follows: power 200~
250W, 40~60min of time；
Step 6: the material after ultrasonication in step 5 is centrifuged, the precipitating and supernatant of acquisition, by supernatant tune
PH4.0~5.0 are saved, are stood, obtain kernel alkali solubility globulin after the sediment being collected after centrifugation is freeze-dried；
Step 7: after the precipitating washing that material centrifugation in step 6 is obtained, according to 1g:(20-40) solid-liquid ratio of mL with
Water mixing, 800-1000 turn/min stirring 60-90min, are then centrifuged for, the supernatant of acquisition is freeze-dried to obtain the clear egg of kernel
White, the precipitating of acquisition is stand-by；
Step 8: then, the precipitating that step 7 is obtained, according to 1g:(20-40) solid-liquid ratio of mL and NaCl solution it is mixed
Close, 800-1000 turns/min stirs 60-90min, and it is kernel salt dissolubility globulin that centrifugation, which obtains supernatant, the precipitating of acquisition to
With；
Step 9: then, the precipitating that step 8 is obtained, according to 1g:(20-40) solid-liquid ratio of mL and ethanol solution it is mixed
It closes, 800-1000 turns/min stirring 60-90min, and it is kernel alcohol soluble protein that centrifugation, which obtains supernatant, and the precipitating of acquisition is stand-by；
Step 10: then, the precipitating that step 9 is obtained, according to 1g:(20-40) solid-liquid ratio of mL and NaOH solution it is mixed
It closes, 800-1000 turns/min stirring 60-90min, and it is kernel glutelin that centrifugation, which obtains supernatant,.
It further limits, polysaccharide residual quantity is 2.0- in the tamarind kernel after extraction polysaccharide described in step 1
3.5g/100g。
It further limits, the mesh number of sieving described in step 1 is 80-120 mesh.
It further limits, polysaccharide residual quantity is 0.5-1.5g/100g in precipitating described in step 2.
It further limits, the ratio of precipitating described in step 3 and buffer solution Tris-HCl are 1g:40mL.
It further limits, the reagent that adjusting pH value described in step 3 uses is food grade sodium hydroxide.
It further limits, the treatment conditions of ultrasonic wave described in step 5 are that power is 200W, time 60min.
It further limits, the reagent that adjusting pH value described in step 6 uses is food grade hydrochloric acid.
It further limits, pH described in step 6 is 4.6.
It further limits, is stood described in step 6, time 30-60min.
It further limits, it is 1g:40mL that the ratio mixed with water is precipitated described in step 7.
It further limits, NaCl solution concentration described in step 8 is 5% (quality), what precipitating was mixed with NaCl solution
Ratio is 1g:40mL.
It further limits, the concentration of ethanol solution described in step 9 is 70% (volume), and precipitating is mixed with ethanol solution
The ratio of conjunction is 1g:40mL.
It further limits, the concentration of NaOH solution described in step 10 is 0.25% (quality), precipitating and NaOH solution
Mixed ratio is 1g:40mL.
Beneficial effect
The present invention provides a kind of tamarind protein extraction processes, have the advantage that compared with prior art
1. the present invention solves the problems, such as that tamarind seed polysaccharide solution is sticky, tamarind protein extraction process can be significant
The dissolution rate of protein is improved, to improve the recovery rate of protein.
2. present invention determine that the isoelectric point of tamarind protein is 4.0-5.0.
3. handling by using ultrasonic wave kernel powder mixed liquor, there is cavitation to kernel powder, it can be effective
Promotion protein separation, to significantly improve the separation rate of polysaccharide and albumen, effectively raise purity of protein, energy
The sliminess for enough reducing protein fills discarded protein to further improve protein extracting ratio
The processing and utilization divided.
4. the present invention goes back while the protein classes and content of tamarind kernel has been determined.
It is the production tamarind protein of high-volume scale 5. the present invention provides broad space for the exploitation of tamarind
Precondition is provided.
Specific embodiment
Raw material, reagent and instrument and equipment of the present invention can be bought by commercialization approach and be obtained.
Embodiment one: tamarind protein extracting method.
It successively carries out as steps described below:
Step 1: will extract the tamarind kernel (polysaccharide residual quantity be 2.25g/100g) after polysaccharide be put into pulverizer into
Row crushes, and sieves with 100 mesh sieve, and obtains kernel powder；
Step 2: kernel powder is mixed with pH3.6 citric acid solution, the ratio of the kernel powder and the mixing of pH3.6 citric acid
Example is 1g:50mL, and 800 turns/min stirs 30min, and centrifugation is repeated 3 times mixing centrifugation, is precipitated, polysaccharide residual quantity in precipitating
For 0.89g/100g；
Step 3: the precipitating in step 2 is mixed with Tris-HCl buffer solution, and precipitating and the ratio of buffer solution are
1g:20mL, addition food grade sodium hydroxide adjust pH11.0, obtain mixed material；
Step 4: mixed material being put into the water-bath for be maintained at 60 DEG C and heated, and stirs 60min；
Step 5: the material after heating stirring is subjected to 200W ultrasonication, time 60min；
Step 6: the material 4000r/min after ultrasonication is centrifuged 15min, with food grade hydrochloric acid tune in supernatant
PH to 4.0 is saved, 30min is stood, 8000r/min is centrifuged 15min and collects sediment freezing for 24 hours, and freeze dryer is dry, obtains tamarind
Kernel alkali solubility albumen；
Step 7: mixed according to the solid-liquid ratio and water of 1g:20mL after the precipitating washing that material centrifugation in step 6 is obtained
It closes, 800 turns/min stirs 90min, is then centrifuged for, and the supernatant of acquisition is freeze-dried to obtain kernel albumin, and acquisition is sunk
It forms sediment stand-by；
Step 8: then, the precipitating that step 7 is obtained, solid-liquid ratio and mass concentration according to 1g:20mL are 5%
NaCl solution mixing, 800 turns/min stir 90min, and it is kernel salt dissolubility globulin that centrifugation, which obtains supernatant, the precipitating of acquisition to
With；
Step 9: then, the precipitating that step 8 is obtained, solid-liquid ratio and volumetric concentration according to 1g:20mL are 70%
Ethanol solution mixing, 800 turns/min stir 90min, and it is kernel alcohol soluble protein that centrifugation, which obtains supernatant, and the precipitating of acquisition is stand-by；
Step 10: then, the precipitating that step 9 is obtained, solid-liquid ratio and mass concentration according to 1g:20mL are 0.25%
NaOH solution mixing, 800 turns/min stir 90min, centrifugation obtain supernatant be kernel glutelin.
Embodiment two: tamarind protein extracting method.
It successively carries out as steps described below:
Step 1: will extract the tamarind kernel (polysaccharide residual quantity be 2.10g/100g) after polysaccharide be put into pulverizer into
Row crushes, and crosses 80 meshes, obtains kernel powder；
Step 2: kernel powder is mixed with pH3.6 citric acid solution, the ratio of the kernel powder and the mixing of pH3.6 citric acid
Example is 1g:30mL, and 1000 turns/min stirs 50min, and centrifugation is repeated 3 times mixing centrifugation, is precipitated, and polysaccharide remains in precipitating
Amount is 1.06g/100g；
Step 3: precipitating is mixed with Tris-HCl buffer solution, and precipitating and the ratio of buffer solution are 1g:30mL, is added
Add food grade sodium hydroxide to adjust pH11.0 and obtains mixed material；
Step 4: mixed material being put into the water-bath for be maintained at 65 DEG C and heated, and stirs 60min；
Step 5: 250W ultrasonication, time 50min are carried out to the material after heating stirring；
Step 6: the material 4000r/min after ultrasonication is centrifuged 15min, with food grade hydrochloric acid tune in supernatant
PH to 4.5 is saved, 60min is stood, 8000r/min is centrifuged 15min and collects sediment freezing for 24 hours, and freeze dryer is dry, obtains tamarind
Kernel alkali solubility albumen；
Step 7: mixed according to the solid-liquid ratio and water of 1g:30mL after the precipitating washing that material centrifugation in step 6 is obtained
It closes, 1000 turns/min stirs 60min, is then centrifuged for, and the supernatant of acquisition is freeze-dried to obtain kernel albumin, and acquisition is sunk
It forms sediment stand-by；
Step 8: then, the precipitating that step 7 is obtained, solid-liquid ratio and mass concentration according to 1g:30mL are 5%
NaCl solution mixing, 1000 turns/min stir 60min, and it is kernel salt dissolubility globulin, the precipitating of acquisition that centrifugation, which obtains supernatant,
For use；
Step 9: then, the precipitating that step 8 is obtained, solid-liquid ratio and volumetric concentration according to 1g:30mL are 70%
Ethanol solution mixing, 1000 turns/min stir 60min, and it is kernel alcohol soluble protein that centrifugation, which obtains supernatant, and the precipitating of acquisition is stand-by；
Step 10: then, the precipitating that step 9 is obtained, solid-liquid ratio and mass concentration according to 1g:30mL are 0.25%
NaOH solution mixing, 1000 turns/min stir 60min, centrifugation obtain supernatant be kernel glutelin.
Embodiment three: tamarind protein extracting method.
It successively carries out as steps described below:
Step 1: will extract the tamarind kernel (polysaccharide residual quantity be 2.02g/100g) after polysaccharide be put into pulverizer into
Row crushes, and crosses 120 meshes, obtains kernel powder；
Step 2: kernel powder is mixed with pH3.6 citric acid solution, the ratio of the kernel powder and the mixing of pH3.6 citric acid
Example is 1g:50mL, and 800 turns/min stirs 30min, and centrifugation is repeated 3 times mixing centrifugation, is precipitated, polysaccharide residual quantity in precipitating
For 0.97g/100g；
Step 3: precipitating is mixed with Tris-HCl buffer solution, and precipitating and the ratio of buffer solution are 1g:40mL, is added
Add food grade sodium hydroxide to adjust pH12.5 and obtains mixed material；
Step 4: mixed material being put into the water-bath for be maintained at 65 DEG C and heated, and stirs 90min；
Step 5: 200W ultrasonication, time 60min are carried out to the material after heating stirring；
Step 6: the material 4000r/min after ultrasonication is centrifuged 15min, with food grade hydrochloric acid tune in supernatant
PH to 4.6 is saved, 30min is stood, 8000r/min is centrifuged 15min and collects sediment freezing for 24 hours, and freeze dryer is dry, obtains tamarind
Kernel alkali solubility globulin.
Step 7: mixed according to the solid-liquid ratio and water of 1g:40mL after the precipitating washing that material centrifugation in step 6 is obtained
It closes, 900 turns/min stirs 80min, is then centrifuged for, and the supernatant of acquisition is freeze-dried to obtain kernel albumin, and acquisition is sunk
It forms sediment stand-by；
Step 8: then, the precipitating that step 7 is obtained, solid-liquid ratio and mass concentration according to 1g:40mL are 5%
NaCl solution mixing, 900 turns/min stir 80min, and it is kernel salt dissolubility globulin that centrifugation, which obtains supernatant, the precipitating of acquisition to
With；
Step 9: then, the precipitating that step 8 is obtained, solid-liquid ratio and volumetric concentration according to 1g:40mL are 70%
Ethanol solution mixing, 900 turns/min stir 80min, and it is kernel alcohol soluble protein that centrifugation, which obtains supernatant, and the precipitating of acquisition is stand-by；
Step 10: then, the precipitating that step 9 is obtained, solid-liquid ratio and mass concentration according to 1g:40mL are 0.25%
NaOH solution mixing, 900 turns/min stir 80min, centrifugation obtain supernatant be kernel glutelin.
Comparative example: tamarind protein extracting method.
Step 1: tamarind kernel is put into and carries out being crushed to 100 mesh in pulverizer；
Step 2: kernel powder (100g) and 1M NaCI solution (800ml) are sufficiently mixed, and add food grade sodium hydroxide
PH value is adjusted to 10；
Step 3: it is stirred at room temperature 30 minutes；
Step 4: by mixture with 6000r/min centrifugation 15 minutes, then with 1M NaC1 solution (200ml) extraction two
It is secondary.It is 4-6 that supernatant, which is adjusted pH with food grade salt, and solid ammonium sulfate (saltouing) is added in saturated solution, is settled out Luo Wang
Sub- protein；Suspension obtains protein precipitation with 8000r/min centrifugation 20min；
Step 5: through 2~4 DEG C of distilled water dialysis 48h, the protein being then freeze-dried in bag filter obtains protein precipitation
To protein concentrate.
The recovery rate of the albumen obtained and pure is extracted according to the GB5099.5-2016 detection present invention by triumphant formula nitriding
Degree, as a result as follows:
1. tamarind kernel alkali solubility protein extracting ratio of table and purity detecting
| Alkali solubility globulin recovery rate % | Purity of protein (%) | Raw material polysaccharide remains (%) | |
| Embodiment one | 60.31% | 61.81% | 2.02 |
| Embodiment two | 65.67% | 66.52% | 2.10 |
| Embodiment three | 68.03% | 68.63% | 2.25 |
| Comparative example | 38.30% | 40.5% | It does not survey |
Seen from table 1, protein is extracted from tamarind kernel using the method for the present invention, recovery rate and purity is more existing mentions
Method is taken to be significantly improved, up to 68% or so.The present invention has also investigated the foaming characteristic of the alkali solubility globulin extracted and has risen
The alkali solubility globulin that extraction obtains is configured to the solution of 10mg/mL, 10000r high speed by taking embodiment 1 as an example by bubble stability
2min is sheared, measures foam volume after foam volume and 30min, the results showed that foaming characteristic and foaming stability are preferable, alkali solubility
The foaming characteristic and foaming stability of globulin are respectively 80%, 75%.
The present invention goes back while other protein classes and content in tamarind kernel has been determined, by taking embodiment three as an example, detection
Other protein contents of tamarind kernel are as follows:
2. embodiment three of table extracts other protein classes of tamarind kernel obtained and content
| Protein classes | Kernel albumin | Salt dissolubility globulin | Kernel alcohol soluble protein | Kernel glutelin |
| Content | 3.18% | 1.31% | 0.44% | 15.31% |
Claims (12)
1. a kind of extracting method of tamarind protein, which is characterized in that successively carry out as steps described below:
Step 1: the tamarind kernel after extraction polysaccharide is crushed, sieving, obtains kernel powder；
Step 2: by kernel powder and pH3.6 citric acid solution according to 1g:(30-50) mL ratio mixing, 800-1000 turn/min
30-50min is stirred, centrifugation is precipitated；
Step 3: the precipitating obtained in step 2 is mixed with Tris-HCl buffer solution, the precipitating and buffer solution Tris-
The ratio of HCl mixing is 1g:(20-40) mL, adjusting pH value is 11.0~13.0, obtains mixed material；
Step 4: mixed material heats 40-90min under the conditions of 60~70 DEG C；
Step 5: and then mixed material is subjected to ultrasonication, the treatment conditions of the ultrasonic wave are as follows: 200~250W of power,
40~60min of time；
Step 6: the material after ultrasonication in step 5 is centrifuged, the precipitating and supernatant of acquisition adjust supernatant
PH4.0~5.0 are stood, and obtain kernel alkali solubility globulin after the sediment being collected after centrifugation is freeze-dried；
Step 7: after the precipitating washing that material centrifugation in step 6 is obtained, according to 1g:(20-40) solid-liquid ratio of mL and water it is mixed
It closing, 800-1000 turns/min stirring 60-90min, is then centrifuged for, and the supernatant of acquisition is freeze-dried to obtain kernel albumin,
The precipitating of acquisition is stand-by；
Step 8: then, the precipitating that step 7 is obtained, according to 1g:(20-40) solid-liquid ratio of mL mixes with NaCl solution,
800-1000 turns/min stirring 60-90min, and it is kernel salt dissolubility globulin that centrifugation, which obtains supernatant, and the precipitating of acquisition is stand-by；
Step 9: then, the precipitating that step 8 is obtained, according to 1g:(20-40) solid-liquid ratio of mL mixes with ethanol solution,
800-1000 turns/min stirring 60-90min, and it is kernel alcohol soluble protein that centrifugation, which obtains supernatant, and the precipitating of acquisition is stand-by；
Step 10: then, the precipitating that step 9 is obtained, according to 1g:(20-40) solid-liquid ratio of mL mixes with NaOH solution,
800-1000 turns/min stirring 60-90min, and it is kernel glutelin that centrifugation, which obtains supernatant,.
2. extracting method according to claim 1, which is characterized in that the tamarind kind after extraction polysaccharide described in step 1
Polysaccharide residual quantity is 2.0-3.5g/100g in benevolence.
3. extracting method according to claim 1, which is characterized in that the mesh number of sieving described in step 1 is 80-120
Mesh.
4. extracting method according to claim 1, which is characterized in that polysaccharide residual quantity is in precipitating described in step 2
0.5-1.5g/100g。
5. extracting method according to claim 1, which is characterized in that precipitating described in step 3 and buffer solution Tris-
The ratio of HCl is 1g:40mL.
6. extracting method according to claim 1, which is characterized in that the treatment conditions of ultrasonic wave described in step 5 are function
Rate is 200W, time 60min.
7. extracting method according to claim 1, which is characterized in that pH described in step 6 is 4.6.
8. extracting method according to claim 1, which is characterized in that stood described in step 6, time 30-60min.
9. extracting method according to claim 1, which is characterized in that precipitating the ratio mixed with water described in step 7 is
1g:40mL.
10. extracting method according to claim 1, which is characterized in that NaCl solution concentration described in step 8 is 5%
(quality), precipitating the ratio mixed with NaCl solution is 1g:40mL.
11. extracting method according to claim 1, which is characterized in that the concentration of ethanol solution described in step 9 is
70% (volume), precipitating the ratio mixed with ethanol solution is 1g:40mL.
12. extracting method according to claim 1, which is characterized in that the concentration of NaOH solution described in step 10 is
0.25% (quality), precipitating the ratio mixed with NaOH solution is 1g:40mL.
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Application publication date: 20191129 |