CN107892722A - A kind of preparation method of water-soluble tamarind seed polysaccharide - Google Patents
A kind of preparation method of water-soluble tamarind seed polysaccharide Download PDFInfo
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- CN107892722A CN107892722A CN201711291711.9A CN201711291711A CN107892722A CN 107892722 A CN107892722 A CN 107892722A CN 201711291711 A CN201711291711 A CN 201711291711A CN 107892722 A CN107892722 A CN 107892722A
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- water
- seed polysaccharide
- tamarind seed
- sediment
- alcohol precipitation
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- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08B—POLYSACCHARIDES; DERIVATIVES THEREOF
- C08B37/00—Preparation of polysaccharides not provided for in groups C08B1/00 - C08B35/00; Derivatives thereof
- C08B37/0003—General processes for their isolation or fractionation, e.g. purification or extraction from biomass
Abstract
The invention discloses a kind of preparation method of water-soluble tamarind seed polysaccharide, comprise the following steps：Step 1, tamarind seeds are pre-processed, obtains pretreated feedstock；Step 2, pretreated feedstock is added into distilled water in predetermined ratio, stirred, pH is adjusted to 3.0 4.0 with citric acid solution, and heating stirring extracts certain time at 80 DEG C, and temperature then is brought up into 100 DEG C, boil 5min and stop heating, cooling and centrifugation obtain extract solution；Step 3, ethanol is added in extract solution, makes ethanol final concentration of 40 60%, alcohol precipitation is carried out, centrifugation, obtains sediment, sediment is continued to be dissolved with water, repeats alcohol precipitation processing, obtains the sediment after multiple alcohol precipitation；Step 4, organic reagent is added into the sediment after multiple alcohol precipitation to carry out dewater treatment and air-dry, the solids obtained after air-drying is crushed, and obtains tamarind seed polysaccharide.
Description
Technical field
The invention belongs to plant polyose to extract field, and in particular to a kind of preparation method of water-soluble tamarind seed polysaccharide.
Background technology
Tamarind, also known as tamarind, it is a kind of tall and big aiphyllium plant of Caesalpiniaceae tamarind category, is mainly distributed in China
In Yunnan, Hainan, Guangxi and other places, aboundresources.Contain non-fiber class polysaccharide component 65-73%, protein in tamarind seeds
15-20%, fatty 6-8%, it can be seen that, the overwhelming majority is polysaccharose substance in tamarind seeds, and the polysaccharide is also known as Luo Wang
Sub- polysaccharide.
Tamarind seed polysaccharide is a kind of edible neutral polysaccharide, compared with other edible polysaccharide, except with stable, breast
Change, thickening, outside water conservation and film forming function, also there is unique physicochemical property, such as good heat-resisting, salt tolerant, acidproof, freeze-resistant and
Defrosting etc., play the role of in the food industry irreplaceable.Abroad, tamarind seed polysaccharide research and application it is existing very long
For a period of time, the industrialized production of the Japanese tamarind seed polysaccharide that takes the lead in realizing, and being sold as food additives.Utilize its increasing
Consistence, gelation, stability and emulsibility, tamarind seed polysaccharide are widely used in ice cream, ice cream, tartar sauce, cooking product, drink
In the production of material etc..It is relevant to report less, related technology because development and application of the China to tamarind seed polysaccharide is started late
Also immature with standard, far behind the U.S. and Japan and other countries, most of tamarind seed polysaccharide product all rely on import.
China's tamarind aboundresources, the development and application of tamarind seed polysaccharide have resource guarantee, meanwhile, China tamarind
The market demand of polysaccharide is big, has a extensive future.2015, China issued country of the tamarind seed polysaccharide glue as food additives
Standard (GB1886.106-2015) is, it is specified that protein content must not be higher than 3% in tamarind seed polysaccharide product.At present, tamarind is more
The product that the extraction of the traditional extraction technique of sugar, such as hot water, organic acid extraction obtain, is extremely difficult to national standard, so needing
Want a kind of preparation method for the tamarind seed polysaccharide that can deviate from protein.
The content of the invention
The present invention is, and it is an object of the present invention to provide a kind of system of water-soluble tamarind seed polysaccharide in order to solving the above problems and carry out
Preparation Method.
The invention provides a kind of preparation method of water-soluble tamarind seed polysaccharide, have the feature that, including following step
Suddenly：
Step 1, tamarind seeds are pre-processed, obtains pretreated feedstock；
Step 2, pretreated feedstock is added into distilled water in predetermined ratio, stirred, pH is adjusted with citric acid solution
To 3.0-4.0, and heating stirring extracts certain time at 80 DEG C, and temperature then is brought up into 100 DEG C, boils 5min and stops adding
Heat, cooling and centrifugation obtain extract solution；
Step 3, ethanol is added in the extract solution, it is 40-60% to make concentration of alcohol, then carries out alcohol precipitation, centrifugation, is obtained
To sediment, the sediment is continued to be dissolved with water, repeat alcohol precipitation processing, obtain the sediment after multiple alcohol precipitation；
Step 4, organic reagent is added into the sediment after multiple alcohol precipitation to carry out dewater treatment and air-dry, after air-drying
To solids crushed, obtain tamarind seed polysaccharide.
In the preparation method of water-soluble tamarind seed polysaccharide provided by the invention, it can also have the feature that：Wherein,
Pretreatment in step 1 includes following sub-step：
Step 1-1, tamarind seeds are soaked, high temperature is dried, broken decortication；
Step 1-2, the tamarind seeds after decortication are crushed, cross 60-80 mesh sieves and obtain fine powder；
Step 1-3, fine powder is soaked in the ethanol solution that concentration is 70-80% and stirs 12h, is centrifuged, drying is precipitated, obtains
To pretreated feedstock.
In the preparation method of water-soluble tamarind seed polysaccharide provided by the invention, it can also have the feature that：Wherein,
Predetermined ratio is 1 in step 2:40-1:50.
In the preparation method of water-soluble tamarind seed polysaccharide provided by the invention, it can also have the feature that：Wherein,
The time that extraction is stirred in step 2 is 15-30min.
In the preparation method of water-soluble tamarind seed polysaccharide provided by the invention, it can also have the feature that：Wherein,
The time of each alcohol precipitation is 2h in step 3.
In the preparation method of water-soluble tamarind seed polysaccharide provided by the invention, it can also have the feature that：Wherein,
The number of alcohol precipitation in step 3 is 2-3 times.
The effect of invention and effect
According to the preparation method of water-soluble tamarind seed polysaccharide involved in the present invention, because using pH value between 3-4
Citric acid solution is extracted to tamarind seed polysaccharide, and passes through multiple alcohol precipitation step, therefore the tamarind seed polysaccharide being prepared
It is less than 3% for white powder and protein content, meets national Specification, and recovery rate is higher than 50%.In addition, citric acid
For water-soluble substances, its own is non-toxic and is a kind of conventional food additives, therefore during the water extraction of the present invention
Do not use and be not suitable for edible additive, obtained tamarind seed polysaccharide more meets food safety requirements.
Generally speaking, the tamarind seed polysaccharide being prepared using the method for the present invention meets national standard, and meets food
The requirement of additive, laid the foundation for the utilization of China's tamarind seed polysaccharide.
Brief description of the drawings
Fig. 1 is the tamarind seed polysaccharide extraction flow chart in embodiments of the invention.
Embodiment
In order that the technological means that the present invention realizes is easy to understand with effect, with reference to embodiments and accompanying drawing is to this
Invention is specifically addressed.
Fig. 1 is the tamarind seed polysaccharide extraction flow chart in embodiments of the invention.
Embodiment one：As shown in figure 1, the step of hot-water process's extraction tamarind seed polysaccharide, is as follows：
Step 1, after tamarind seeds are soaked in water, heated-air drying, decortication is crushed, crushed, crossed 60 mesh sieves, obtain fine powder；
It is appropriate to weigh tamarind fine powder, by 1:15 solid-liquid ratios add 75% ethanol, stir 12h, centrifugation, precipitate drying, obtain ethanol
Dried powder afterwards；
Step 2,100g dried powders are weighed, by solid-liquid ratio 1:45 add distilled water, and 80 DEG C of heating stirrings extract 20min,
Then temperature is brought up to 100 DEG C, boils 5min and stop heating, cooling and centrifugation obtain extract solution.
Step 3, ethanol is slowly added in extract solution, makes ethanol final concentration of 50%, alcohol precipitation 2h is stood, centrifugation, obtains
Sediment, sediment is continued to be dissolved with water, repeat 2 alcohol precipitation processing of progress, obtain the sediment after 3 alcohol precipitations.
Step 4, isopropanol is added into the sediment after 3 alcohol precipitations to carry out dewater treatment and air-dry, and is obtained after air-drying
Solids crushed, obtain hot water extraction tamarind seed polysaccharide A.
The recovery rate of tamarind seed polysaccharide A obtained by the present embodiment is 55%, and khaki powder is tasteless, and protein content is
6.7%, higher than the 3% of national Specification.
Embodiment two：As shown in figure 1, the step of citric acid solution (pH 4.6) extraction tamarind seed polysaccharide, is as follows：
Step 1, after tamarind seeds are soaked in water, heated-air drying, decortication is crushed, crushed, crossed 60 mesh sieves, obtain fine powder；
It is appropriate to weigh tamarind fine powder, by 1:15 solid-liquid ratios add 75% ethanol, stir 12h, centrifugation, precipitate drying, obtain ethanol
Dried powder afterwards；
Step 2,100g dried powders are weighed, by solid-liquid ratio 1:45 add distilled water, are adjusted with High Content Citric Acid solution
PH is to 4.6, and heating stirring extracts 15min at 80 DEG C, and temperature then is brought up into 100 DEG C, boils 5min and stops heating, cold
But extract solution is obtained with centrifugation.
Step 3, ethanol is slowly added into extract solution, makes ethanol final concentration of 50%, alcohol precipitation 2h is stood, centrifugation, obtains
Sediment, sediment is continued to be dissolved with water, repeat 2 alcohol precipitation processing of progress, obtain the sediment after 3 alcohol precipitations.
Step 4, isopropanol is added into the sediment after 3 alcohol precipitations to carry out dewater treatment and air-dry, and is obtained after air-drying
Solids crushed, obtain citric acid solution extraction tamarind seed polysaccharide B.
The recovery rate of tamarind seed polysaccharide B obtained by the present embodiment is 51%, and pale yellow powder is tasteless, and protein content is
3.8%, higher than the 3% of national Specification.
Embodiment three：As shown in figure 1, the step of citric acid solution (pH 4.0) extraction tamarind seed polysaccharide, is as follows：
Step 1, after tamarind seeds are soaked in water, heated-air drying, decortication is crushed, crushed, crossed 60 mesh sieves, obtain fine powder；
It is appropriate to weigh tamarind fine powder, by 1:15 solid-liquid ratios add 75% ethanol, stir 12h, centrifugation, precipitate drying, obtain ethanol
Dried powder afterwards.
Step 2,100g dried powders are weighed, by solid-liquid ratio 1:45 add distilled water, are adjusted with High Content Citric Acid solution
PH is to 4.0, and heating stirring extracts 25min at 80 DEG C, and temperature then is brought up into 100 DEG C, boils 5min and stops heating, cold
But extract solution is obtained with centrifugation.
Step 3, ethanol is added in extract solution, it is 40% to make concentration of alcohol, then stands alcohol precipitation 2h, centrifugation, is sunk
Starch, sediment is continued to be dissolved with water, repeat 2 alcohol precipitation processing of progress, obtain the sediment after 3 alcohol precipitations.
Step 4, isopropanol is added into the sediment after 3 alcohol precipitations to carry out dewater treatment and air-dry, and is obtained after air-drying
Solids crushed, obtain citric acid solution extraction tamarind seed polysaccharide C.
The recovery rate of tamarind seed polysaccharide C obtained by the present embodiment is 50%, and white powder is tasteless, and protein content is
2.9%, meet national Specification is less than 3%.
Example IV：As shown in figure 1, the step of citric acid solution (pH 3.6) extraction tamarind seed polysaccharide, is as follows：
Step 1, after tamarind seeds are soaked in water, heated-air drying, decortication is crushed, crushed, crossed 60 mesh sieves, obtain fine powder；
It is appropriate to weigh tamarind fine powder, by 1:15 solid-liquid ratios add 75% ethanol, stir 12h, centrifugation, precipitate drying, obtain ethanol
Dried powder afterwards.
Step 2,100g dried powders are weighed, by solid-liquid ratio 1:40 add distilled water, are adjusted with High Content Citric Acid solution
PH is to 3.6, and heating stirring extracts 30min at 80 DEG C, and temperature then is brought up into 100 DEG C, boils 5min and stops heating, cold
But extract solution is obtained with centrifugation.
Step 3, ethanol is added in extract solution, makes ethanol final concentration of 60%, alcohol precipitation 2h is then stood, centrifugation, obtains
Sediment, sediment is continued to be dissolved with water, repeat 2 alcohol precipitation processing of progress, obtain the sediment after 3 alcohol precipitations.
Step 4, isopropanol is added into the sediment after 3 alcohol precipitations to carry out dewater treatment and air-dry, and is obtained after air-drying
Solids crushed, obtain citric acid solution extraction tamarind seed polysaccharide D.
The recovery rate of tamarind seed polysaccharide D obtained by the present embodiment is 52%, and white powder is tasteless, and protein content is
2.7%, meet national Specification is less than 3%.
Embodiment five：As shown in figure 1, the step of citric acid solution (pH 3.1) extraction tamarind seed polysaccharide, is as follows：
Step 1, after tamarind seeds are soaked in water, heated-air drying, decortication is crushed, crushed, crossed 60 mesh sieves, obtain fine powder；
It is appropriate to weigh tamarind fine powder, by 1:15 solid-liquid ratios add 75% ethanol, stir 12h, centrifugation, precipitate drying, obtain ethanol
Dried powder afterwards.
Step 2,100g dried powders are weighed, by solid-liquid ratio 1:50 add distilled water, are adjusted with High Content Citric Acid solution
PH is to 3.1, and heating stirring extracts 20min at 80 DEG C, and temperature then is brought up into 100 DEG C, boils 5min and stops heating, cold
But extract solution is obtained with centrifugation.
Step 3, ethanol is slowly added in extract solution, makes ethanol final concentration of 50%, alcohol precipitation 2h is stood, centrifugation, obtains
Sediment, sediment is continued to be dissolved with water, repeat 2 alcohol precipitation processing of progress, obtain the sediment after 3 alcohol precipitations.
Step 4, isopropanol is added into the sediment after 3 alcohol precipitations to carry out dewater treatment and air-dry, and is obtained after air-drying
Solids crushed, obtain citric acid solution extraction tamarind seed polysaccharide E.
The recovery rate of tamarind seed polysaccharide E obtained by the present embodiment is 52%, and white powder is tasteless, and protein content is
2.8%, meet national Specification is less than 3%.
The effect of embodiment and effect
The tamarind seed polysaccharide for understanding, when extracting tamarind seed polysaccharide using hot-water process, to be prepared according to embodiment one is soil
Yellow powder and protein content are 6.7% higher than the 3% of national Specification.
It can be seen from embodiment two, when being extracted using pH4.6 citric acid solution, the tamarind being prepared is more
Sugar be pale yellow powder and protein content be 3.8% slightly above national Specification 3%, when showing to use citric acid solution
The color of tamarind seed polysaccharide can be made partially light, protein content reduces.
It can be seen from embodiment three, when being extracted using pH4.0 citric acid solution, the tamarind being prepared is more
Sugar be white powder and protein content be 2.9% be less than national Specification 3%, show to make when using citric acid solution
The color of tamarind seed polysaccharide is white, and protein content reduces.
It can be seen from example IV, when being extracted using pH3.6 citric acid solution, the tamarind being prepared is more
Sugar be white powder and protein content be 2.7% be less than national Specification 3%, show to make when using citric acid solution
The color of tamarind seed polysaccharide is white, and protein content reduces.
It can be seen from embodiment five, when being extracted using pH3.1 citric acid solution, the tamarind being prepared is more
Sugar be white powder and protein content be 2.8% be less than national Specification 3%, show to make when using citric acid solution
The color of tamarind seed polysaccharide is white, and protein content reduces.
It can be seen from the preparation method of the water-soluble tamarind seed polysaccharide involved by embodiment one to embodiment five, when using lemon
When lemon acid solution is extracted, the tamarind seed polysaccharide being prepared is marked for white or yellow powder and protein content less than country
3% as defined in standard, recovery rate is higher than 50%.Further, when use citric acid solution pH value between 3-4 when, be prepared into
The tamarind seed polysaccharide arrived is white powder, and protein content is less than 3%, meets national Specification, and recovery rate is higher than 50%.
Therefore, extracted using citric acid solution and citric acid solution pH value is set to 3-4, it becomes possible to ensureing that recovery rate is higher than
Protein content is obtained while 50% and is less than national standard and color more preferably tamarind seed polysaccharide.
Above-mentioned embodiment is the preferred case of the present invention, is not intended to limit protection scope of the present invention.
In above-described embodiment one to five, the organic reagent used in step 4 is isopropanol, but this has in the present invention
Machine reagent can also be the volatile reagent such as ether.
Claims (6)
1. a kind of preparation method of water-soluble tamarind seed polysaccharide, it is characterised in that comprise the following steps：
Step 1, tamarind seeds are pre-processed, obtains pretreated feedstock；
Step 2, the pretreated feedstock is added into distilled water in predetermined ratio, stirred, with High Content Citric Acid solution
PH is adjusted to 3.0-4.0, and heating stirring extracts certain time at 80 DEG C, and temperature then is brought up into 100 DEG C, boils 5min
Stop heating, cooling and centrifugation obtain extract solution；
Step 3, ethanol is added in the extract solution, makes the final concentration of 40-60% of ethanol, then carry out alcohol precipitation, centrifuged, obtain
Sediment, the sediment is continued to be dissolved with water, repeat alcohol precipitation processing, obtain the sediment after multiple alcohol precipitation；
Step 4, organic reagent is added into the sediment after the repeatedly alcohol precipitation to carry out dewater treatment and air-dry, after air-drying
To solids crushed, obtain tamarind seed polysaccharide.
2. the preparation method of water-soluble tamarind seed polysaccharide according to claim 1, it is characterised in that：
Wherein, the pretreatment in the step 1 includes following sub-step：
Step 1-1, the tamarind seeds are soaked, high temperature is dried, broken decortication；
Step 1-2, the tamarind seeds after decortication are crushed, cross 60-80 mesh sieves and obtain fine powder；
Step 1-3, the fine powder is soaked in the ethanol solution that concentration is 70-80% and stirs 12h, is centrifuged, drying is precipitated, obtains
To the pretreated feedstock.
3. the preparation method of water-soluble tamarind seed polysaccharide according to claim 1, it is characterised in that：
Wherein, the predetermined ratio in the step 2 is 1:40-1:50.
4. the preparation method of water-soluble tamarind seed polysaccharide according to claim 1, it is characterised in that：
Wherein, the time of the stirring extraction in the step 2 is 15-30min.
5. the preparation method of water-soluble tamarind seed polysaccharide according to claim 1, it is characterised in that：
Wherein, the time of each alcohol precipitation is 2h in the step 3.
6. the preparation method of water-soluble tamarind seed polysaccharide according to claim 1, it is characterised in that：
Wherein, the number of the alcohol precipitation in the step 3 is 2-3 times.
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN110511264A (en) * | 2019-09-16 | 2019-11-29 | 云南猫哆哩集团食品有限责任公司 | A kind of extracting method of tamarind protein |
| CN112794926A (en) * | 2021-02-02 | 2021-05-14 | 武汉芘芘薇莎生物科技有限公司 | Extraction method and application of tamarind seed polysaccharide |
| CN114181329A (en) * | 2021-12-27 | 2022-03-15 | 青岛琛蓝海洋生物工程有限公司 | Method for extracting xyloglucan in tamarind seeds |
| CN114304547A (en) * | 2021-12-15 | 2022-04-12 | 云南猫哆哩集团食品有限责任公司 | Preparation method of tamarind and dietary fiber mixed fruit and vegetable crisp chips |
| CN114376064A (en) * | 2021-12-22 | 2022-04-22 | 云南猫哆哩集团食品有限责任公司 | Preparation method of cross-linked tamarind seed polysaccharide gum, ice sucker stabilizer and application of ice sucker stabilizer |
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Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110511264A (en) * | 2019-09-16 | 2019-11-29 | 云南猫哆哩集团食品有限责任公司 | A kind of extracting method of tamarind protein |
| CN112794926A (en) * | 2021-02-02 | 2021-05-14 | 武汉芘芘薇莎生物科技有限公司 | Extraction method and application of tamarind seed polysaccharide |
| CN114304547A (en) * | 2021-12-15 | 2022-04-12 | 云南猫哆哩集团食品有限责任公司 | Preparation method of tamarind and dietary fiber mixed fruit and vegetable crisp chips |
| CN114376064A (en) * | 2021-12-22 | 2022-04-22 | 云南猫哆哩集团食品有限责任公司 | Preparation method of cross-linked tamarind seed polysaccharide gum, ice sucker stabilizer and application of ice sucker stabilizer |
| CN114181329A (en) * | 2021-12-27 | 2022-03-15 | 青岛琛蓝海洋生物工程有限公司 | Method for extracting xyloglucan in tamarind seeds |
| CN114181329B (en) * | 2021-12-27 | 2022-11-15 | 青岛琛蓝海洋生物工程有限公司 | Method for extracting xyloglucan in tamarind seeds |
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Application publication date: 20180410 |