CN101585873A - From waste sea cucumber processing liquid, reclaim the method for protein and Crude polysaccharides - Google Patents
From waste sea cucumber processing liquid, reclaim the method for protein and Crude polysaccharides Download PDFInfo
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- CN101585873A CN101585873A CNA2009100124253A CN200910012425A CN101585873A CN 101585873 A CN101585873 A CN 101585873A CN A2009100124253 A CNA2009100124253 A CN A2009100124253A CN 200910012425 A CN200910012425 A CN 200910012425A CN 101585873 A CN101585873 A CN 101585873A
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Abstract
The present invention discloses a kind of method that reclaims protein and Crude polysaccharides from waste sea cucumber processing liquid, is to get sea cucumber machining cooking liquid stoste earlier; With hydrochloric acid or citric acid solution transfer sea cucumber cooking liquor stoste to pH4.5～5.5; Centrifugal throw out and the supernatant liquor of obtaining; Adding sodium hydroxide solution and be neutralized to pH7 in the gained throw out, is the dialysis tubing dialysis desalination of 3000～5000kd with the molecular weight that dams, residue in the lyophilize dialysis tubing, the mixture of protein and Crude polysaccharides; In the gained supernatant liquor, add sodium hydroxide solution and be neutralized to pH7, add ratio with the supernatant liquor volume more successively and be 30%, 50%, 70%, concentration is that 95% ethanol carries out the classification alcohol precipitation, the each adding behind the ethanol all, isolate precipitation and collect with 3000～8000 rev/mins of centrifugal 20～40min down; Merge the gained throw out, heating concentrates and ethanol evaporation in 40～50 ℃ thermostat water bath, and the lyophilize enriched material gets the Crude polysaccharides powder then.
Description
Technical field:
The present invention relates to a kind of recoverying and utilizing method of waste sea cucumber processing liquid, especially a kind of technology is simple, cost is low, the rate of recovery is high, can improve the recovery protein from waste sea cucumber processing liquid of cucumber product processing added value and the method for Crude polysaccharides.
Background technology:
Sea cucumber (Sea cucumbers; holothurians) belong to the Mollusca Holothuroidea; nutritious; except that the high protein of content; also have element such as 18 seed amino acids, taurine, chondroitin sulfate, sea cucumber acidic mucopolysaccharide, calcium, phosphorus, iron, iodine, zinc, selenium, vanadium, manganese and VITMAIN B1, Wei ShengsuB2, nicotinic acid etc.; be the nutritive health-care food of rare in the world high protein, lower fat, low sugar, no cholesterol, be listed in one of " marine products eight delicacies ".Though bright sea cucumber is delicious and be of high nutritive value, but, it is difficult to preserve because of containing autolytic enzyme, so all needing to carry out the boiling enzyme that goes out mostly for bright sea cucumber, prior art handles, and then be processed into various cucumber products (instant sea cucumber, salt marsh sea cucumber, dry Stichopus japonicus) and preserve, simultaneously, increase in order to make sea cucumber water send out the back volume, help digestion, most cucumber products all need long boiling.Must cause the interior contained soluble nutritious components (as protein, sea cucumber acidic mucopolysaccharide etc.) of sea cucumber body wall to be dissolved in the cooking liquor.The content of Crude polysaccharides is 150～300mg/1000ml in the sea cucumber machining cooking liquid after testing, and the content of crude protein is 10～30g/1000ml.Proteinic nutritive value is well-known; The functionally active of sea cucumber acidic mucopolysaccharide (sea cucumber polysaccharide) also further confirmed, it has different physiological roles such as strengthening immunity, antitumor, anticoagulant, anti-ageing, anti-radiation.The cooking liquor of the existing cucumber product of cause is all as discarded liquid, and wherein protein and sea cucumber polysaccharide run off thereupon, cause the wasting of resources.
Summary of the invention:
The present invention is in order to solve the above-mentioned technical problem of existing in prior technology, provides that a kind of technology is simple, cost is low, the rate of recovery is high, can improve the method that reclaims protein and Crude polysaccharides from waste sea cucumber processing liquid of cucumber product processing added value.
Technical solution of the present invention is: a kind of method that reclaims protein and Crude polysaccharides from waste sea cucumber processing liquid is characterized in that carrying out according to the following steps:
A. get sea cucumber machining cooking liquid, cooling is left standstill and is removed sandstone impurity, gets sea cucumber machining cooking liquid;
B. transfer sea cucumber cooking liquor stoste to pH4.5～5.5 with hydrochloric acid or the citric acid solution of 0.5mol/l～10mol/l;
C. in 4～10 ℃ of environment, 3000～6000 rev/mins centrifugal 20～30 minutes, obtain throw out and supernatant liquor;
D. the sodium hydroxide solution that adds 0.5mol/l～10mol/l in the gained throw out is neutralized to pH7, it with the molecular weight that dams the dialysis tubing of the 3000～5000kd 48h desalination of in 4 ℃～5 ℃ environment, dialysing, residue in the lyophilize dialysis tubing promptly gets powders A;
E. the sodium hydroxide solution that adds 0.5mol/l～10mol/l in the gained supernatant liquor is neutralized to pH7, add ratio with the supernatant liquor volume more successively and be 30%, 50%, 70%, concentration is that 95% ethanol carries out the classification alcohol precipitation, the each adding behind the ethanol all, isolate precipitation and collect with 3000～8000 rev/mins of centrifugal 20～40min down;
F. merge e step gained throw out, heating concentrates and ethanol evaporation in 40～50 ℃ thermostat water bath, and the lyophilize enriched material gets powder B then.
The present invention is protein and the Crude polysaccharides that adopts in the discarded liquid of method recovery sea cucumber that acid is heavy and the classification alcohol precipitation combines, not only used material cost is low, equipment and technology are simple, greatly reduce the processing cost of waste sea cucumber processing liquid, main is sea cucumber polysaccharide rate of recovery height, can reach 60～75%, also recyclable simultaneously protein, protein recovery can reach 65%～75%, has effectively improved the added value of cucumber product processing.
Embodiment:
A. get sea cucumber machining cooking liquid, cooling is left standstill and is removed sandstone impurity, gets sea cucumber machining cooking liquid stoste;
B. the hydrochloric acid or the citric acid solution that in sea cucumber machining cooking liquid stoste, add 0.5mol/l～10mol/l, stir while adding, the pH value of sea cucumber cooking liquor stoste is transferred to the iso-electric point place, be pH4.5～5.5, at this moment, protein and being embedded between the protein molecule or with protein molecule bonded polysaccharide just is precipitated out from solution;
C. in equipment such as refrigerator or middle low-temperature cold store, under 4～10 ℃ of conditions, 3000～6000 rev/mins centrifugal 20～30 minutes, obtain throw out and supernatant liquor;
D. the sodium hydroxide solution that adds 0.5mol/l～10mol/l in the gained throw out is neutralized to pH7, the dialysis tubing that with the molecular weight that dams is 3000～5000kd is in refrigerator or middle low-temperature cold store, 48h dialyses under 4 ℃～5 ℃ conditions, salt and partial impurities are passed through dialysis tubing, lyophilize sees through the residue of dialysis tubing, gets powders A;
E. the sodium hydroxide solution that adds 0.5mol/l～10mol/l in the gained supernatant liquor is neutralized to pH7, add ratio with the supernatant liquor volume more successively and be 30%, 50%, 70%, concentration is that 95% ethanol carries out the classification alcohol precipitation, the each adding behind the ethanol all, isolate precipitation and collect with 3000～8000 rev/mins of centrifugal 20～40min down;
F. merge e step gained throw out, heating concentrates and ethanol evaporation in 40～50 ℃ thermostat water bath, and the lyophilize enriched material gets powder B then.
Adopt the protein content in Kjeldahl determination or the Coomassie brilliant blue method mensuration powders A, trying to achieve protein recovery (accounting for sea cucumber machining cooking liquid stoste total protein content) is 65%～75%; Adopt the phenol sulfuric acid process to measure total sugar content in the powders A, adopt 3, reducing sugar content in the 5-dinitrosalicylic acid colorimetric method for determining powders A, Crude polysaccharides content equals total sugar content and deducts reducing sugar content, obtains the Crude polysaccharides rate of recovery in the powders A (accounting for the total Crude polysaccharides content of sea cucumber machining cooking liquid stoste) and can reach 40～45%; Behind the supernatant process classification alcohol precipitation, 90%, 90%, 55% and 80% of main active polysaccharide such as Fucose, uronic acid, aminosugar and sulfate radical precipitated, and records the Crude polysaccharides rate of recovery among the powder B (accounting for the total Crude polysaccharides content of sea cucumber machining cooking liquid stoste) with aforesaid method and can reach 20～30%.
The present invention not only obtains protein but also has obtained a large amount of polysaccharide after peracid is heavy, helps further classification alcohol precipitation recovery polysaccharide from supernatant liquor.Behind the heavy and classification alcohol precipitation, the rate of recovery of Crude polysaccharides can reach 60～75% through peracid, and protein recovery can reach 65%～75%, and products obtained therefrom can be used as functional component to be added in the protective foods (as beche-de-mer capsules).
Claims (1)
1. method that reclaims protein and Crude polysaccharides from waste sea cucumber processing liquid is characterized in that carrying out according to the following steps:
A. get sea cucumber machining cooking liquid, cooling is left standstill and is removed sandstone impurity, gets sea cucumber machining cooking liquid stoste;
B. transfer sea cucumber cooking liquor stoste to pH4.5～5.5 with hydrochloric acid or the citric acid solution of 0.5mol/l～10mol/l;
C. in 4～10 ℃ of environment, 3000～6000 rev/mins centrifugal 20～30 minutes, obtain throw out and supernatant liquor;
D. the sodium hydroxide solution that adds 0.5mol/l～10mol/l in the gained throw out is neutralized to pH7, is the dialysis tubing of the 3000～5000kd 48h desalination of dialyse in 4 ℃～5 ℃ environment with the molecular weight that dams, and residue in the lyophilize dialysis tubing must powders A;
E. the sodium hydroxide solution that adds 0.5mol/l～10mol/l in the gained supernatant liquor is neutralized to pH7, add ratio with the supernatant liquor volume more successively and be 30%, 50%, 70%, concentration is that 95% ethanol carries out the classification alcohol precipitation, the each adding behind the ethanol all, isolate precipitation and collect with 3000～8000 rev/mins of centrifugal 20～40min down;
F. merge e step gained throw out, heating concentrates and ethanol evaporation in 40～50 ℃ thermostat water bath, and the lyophilize enriched material gets powder B then.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN200910012425A CN101585873B (en) | 2009-07-09 | 2009-07-09 | Method for reclaiming protein and crude polysaccharide from waste sea cucumber processing liquid |
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| CN200910012425A CN101585873B (en) | 2009-07-09 | 2009-07-09 | Method for reclaiming protein and crude polysaccharide from waste sea cucumber processing liquid |
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| CN101585873A true CN101585873A (en) | 2009-11-25 |
| CN101585873B CN101585873B (en) | 2012-10-03 |
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Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102406048A (en) * | 2011-11-29 | 2012-04-11 | 山东好当家海洋发展股份有限公司 | Method for preparing sea cucumber glycoprotein by using sea cucumber blanching solution |
| CN102813240A (en) * | 2012-08-28 | 2012-12-12 | 青岛琅琊龙湾海参有限公司 | Mixed sea cucumber powder and production method thereof |
| CN104402983A (en) * | 2014-12-11 | 2015-03-11 | 山东省科学院生物研究所 | Method for extracting glycoprotein by utilizing sea cucumber treatment blanching liquid and application thereof |
| CN105661201A (en) * | 2016-01-20 | 2016-06-15 | 中国海洋大学 | Process for preparing nutritious beverage by aid of sea cucumber cooking liquid |
| CN107892723A (en) * | 2017-12-08 | 2018-04-10 | 上海理工大学 | The processing method of protein content in a kind of reduction tamarind seed polysaccharide |
| CN110590967A (en) * | 2019-08-27 | 2019-12-20 | 中国海洋大学 | Method for recovering organic matters rich in sea cucumber polysaccharide from sea cucumber blanching liquid |
| CN113135981A (en) * | 2021-03-26 | 2021-07-20 | 大连工业大学 | Preparation method and application of sea cucumber protein for promoting wound healing |
Family Cites Families (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1313479C (en) * | 2005-07-17 | 2007-05-02 | 中国海洋大学 | Method for preparing holothurin from waste solution of sea cucumber processing |
| CN101343332A (en) * | 2008-08-20 | 2009-01-14 | 山东省科学院生物研究所 | Method for extracting holothurian polyoses with holothurian processing waste liquor |
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2009
- 2009-07-09 CN CN200910012425A patent/CN101585873B/en active Active
Cited By (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102406048A (en) * | 2011-11-29 | 2012-04-11 | 山东好当家海洋发展股份有限公司 | Method for preparing sea cucumber glycoprotein by using sea cucumber blanching solution |
| CN102406048B (en) * | 2011-11-29 | 2013-04-17 | 山东好当家海洋发展股份有限公司 | Method for preparing sea cucumber glycoprotein by using sea cucumber blanching solution |
| CN102813240A (en) * | 2012-08-28 | 2012-12-12 | 青岛琅琊龙湾海参有限公司 | Mixed sea cucumber powder and production method thereof |
| CN102813240B (en) * | 2012-08-28 | 2013-11-06 | 青岛琅琊龙湾海参有限公司 | Mixed sea cucumber powder and production method thereof |
| CN104402983A (en) * | 2014-12-11 | 2015-03-11 | 山东省科学院生物研究所 | Method for extracting glycoprotein by utilizing sea cucumber treatment blanching liquid and application thereof |
| CN104402983B (en) * | 2014-12-11 | 2017-10-27 | 山东省科学院生物研究所 | The method and purposes of glycoprotein are extracted using Holothurian machining blanching solution |
| CN105661201A (en) * | 2016-01-20 | 2016-06-15 | 中国海洋大学 | Process for preparing nutritious beverage by aid of sea cucumber cooking liquid |
| CN107892723A (en) * | 2017-12-08 | 2018-04-10 | 上海理工大学 | The processing method of protein content in a kind of reduction tamarind seed polysaccharide |
| CN110590967A (en) * | 2019-08-27 | 2019-12-20 | 中国海洋大学 | Method for recovering organic matters rich in sea cucumber polysaccharide from sea cucumber blanching liquid |
| CN113135981A (en) * | 2021-03-26 | 2021-07-20 | 大连工业大学 | Preparation method and application of sea cucumber protein for promoting wound healing |
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| Publication number | Publication date |
|---|---|
| CN101585873B (en) | 2012-10-03 |
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Address after: 116023 Heishijiao Street, Shahekou District, Dalian City, Liaoning Province, 2-52 Patentee after: DALIAN OCEAN University Address before: Heishijiao street Shahekou Dalian District 116000 of Liaoning Province, No. 52 (Dalian Fisheries University) Patentee before: Dalian Fisheries University |
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Effective date of registration: 20190823 Address after: 116000 No. 14 Fuxi Street, Pikou Street, Pulandian District, Dalian City, Liaoning Province Patentee after: DALIAN ZHENJIU BIOLOGICAL INDUSTRY Co.,Ltd. Address before: 116023 Heishijiao Street, Shahekou District, Dalian City, Liaoning Province, 2-52 Patentee before: Dalian Ocean University |