CN107853703A - A kind of preparation method for being used to alleviate the preparation of physical fatigue - Google Patents

A kind of preparation method for being used to alleviate the preparation of physical fatigue Download PDF

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Publication number
CN107853703A
CN107853703A CN201711114016.5A CN201711114016A CN107853703A CN 107853703 A CN107853703 A CN 107853703A CN 201711114016 A CN201711114016 A CN 201711114016A CN 107853703 A CN107853703 A CN 107853703A
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preparation
group
alleviate
physical fatigue
extract solution
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钟宇富
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Broad Grain Hall Herbaceous Biological Science And Technology (shanghai) Co Ltd
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Broad Grain Hall Herbaceous Biological Science And Technology (shanghai) Co Ltd
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Publication of CN107853703A publication Critical patent/CN107853703A/en
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    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
    • A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
    • A23L33/105—Plant extracts, their artificial duplicates or their derivatives
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
    • A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
    • A23L33/15—Vitamins
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
    • A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
    • A23L33/16—Inorganic salts, minerals or trace elements
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
    • A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
    • A23L33/17—Amino acids, peptides or proteins
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs

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  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Mycology (AREA)
  • Health & Medical Sciences (AREA)
  • Nutrition Science (AREA)
  • Engineering & Computer Science (AREA)
  • Food Science & Technology (AREA)
  • Polymers & Plastics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Botany (AREA)
  • Inorganic Chemistry (AREA)
  • Medicines Containing Plant Substances (AREA)

Abstract

The invention discloses a kind of preparation method for being used to alleviate the preparation of physical fatigue, specifically comprise the following steps：A, raspberry, sealwort, radix polygonati officinalis, spina date seed, matrimony vine and jujube are taken by prescription portion rate, added water to cook 3 times, each 1h, extract solution is made；B, obtained extract solution in above-mentioned steps a is collected, filtration, the extract solution of 3 times is merged, concentrating the filtrate to needs concentration, is cooled to room temperature；C, using the extracting method of water extraction and alcohol precipitation method, it is slowly added to ethanol that concentration is 95% and quickly stirs, the alcohol content for making extract solution is 50%, and 12h is placed in sealing, centrifuges to obtain supernatant, reclaims ethanol；D, supernatant is collected, through being dried under reduced pressure at a temperature of 60 DEG C to dry cream, plant extracts is made after crushing；E, particle product or powder is made in plant extracts.The present invention have alleviate physical fatigue and improve energy healthcare function, can enriching yin and nourishing kidney, QI invigorating analgesia, keep or enhancing ovarian function, no dependence.

Description

A kind of preparation method for being used to alleviate the preparation of physical fatigue
Technical field
The invention belongs to food technology field, particularly relates to a kind of preparation for being used to alleviate the preparation of physical fatigue Method.
Background technology
Fatigue is that body is crossed and used or function caused by transfiniting reduces and the state of body discomfort occurs, is mainly shown as god Tired power, have a dizzy spell, insomnia and dreamful sleep, the symptom such as tinnitus is forgetful, have a pain in the back, epilation, poliosis and women's early ageing. If cannot recover in time after fatigue generation, gradually accumulation, can cause to overwork, and body occurs under endocrine disturbance, immunity Drop, or even there is organic disease, it can seriously pose a health risk.Modern medicine proves that fatigue can cause human internal environment to dislike Change, free radical increases, cell ageing, organ dysfunction decline.And human body cell crosses presenility, decline etc. is to lead to organ dysfunction too early Cause the important root of people's aging.
At present, commercially available antifatigue chemicals and biological products such as amphetamine, meclofenoxate, Mo Dafeini, coffee Coffee because, polyactin etc. delay fatigue occur and dispelling fatigue in terms of have preferable effect, but occur many bad anti- Should, such as cardiovascular harmful effect, disturb sleep, be abalienation, additive, causing clinical application to limit to.In Chinese tradition Medicine payes attention to transferring the immunity of human body itself, reaches anti-fatigue ability, and to antifatigue, raising energy, anti-aging aspect has Advantageous advantage, while there is the characteristics of safe and effective, toxic side effect is small.Therefore, develop with toxic side effect it is small, Curative effect is good, can be long-term use of the advantages that, can alleviate physical fatigue, improve the Chinese medicine and health food of energy by with huge city Field development potentiality.
Modern Chinese medicine thinks that fatigue is a name of disease, there is its etiology and pathogenesis, is clinically common disease, frequently-occurring disease, is necessary The new disease paid attention to, is attributed to inferior health category, is related to the vital organs of the human body, mainly based on spleen, liver, kidney.Its tired disease mainly shows For qi-blood deficiency, weakness of the spleen and the stomach, deficiency of kidney-essence, yin asthenia generating intrinsic heat, therefore treat fatigue and aging should be based on " qi-restoratives ", suitable apparatus There are the Chinese medicine or Chinese prescription of qi-blood tonifying, strengthening the spleen and stomach, kidney tonifying, essence replenishing, nourishing Yin and clearing heat and other effects.
Traditional Chinese medicine thinks that the kidney being the origin of congenital constitution, evaporates kidney by kidney yang again for the root of organ yin-yang, kidney controlling essence storage, kidney essense The moon produces kidney qi, and kidney qi can subsidize, promote, coordinating the negative and positive of whole body internal organs, and determines the growth of human body, development, reproduction, declines Always, dead life process.Each process grow or the warp of the situation that fails, especially women, band, tire, production etc. are special Physiology course it is whether normal, both depend on the prosperity and decline profit and loss of kidney essense and kidney qi.Theory of traditional Chinese medical science thinks that kidney deficiency is mainly reflected in waist Knee joint soreness, leg are soft, Hiccough and deaf, tooth falling out, insomnia and dreamful sleep, hectic fever night sweat, hypogona dism, man is simultaneous sees seminal emission, woman Through less, Amenorrhea or early ageing etc., its symptom and fatigue in modern medical theory or the symptom basic one of chronic fatigue syndrome Cause, be all the performance of human physiology hypofunction.Therefore, want to alleviate physical fatigue, improve energy, health care and prevent aging Deng should all start with conditioning from tonic kidney essense kidney qi.
The present invention is according to 6 kinds of medicine foods such as theory of traditional Chinese medical science reasonable compatibility raspberry, sealwort, radix polygonati officinalis, spina date seed, matrimony vine, jujube Homologous medicinal material.Prescription has the effect of same tonifying Qi god, YIN and YANG balance regulating, QI invigorating righting, filling liver kidney, can alleviate CKD and become and each The card of kidney qi deficiency, alleviates physical fatigue caused by kind disease, improves human body energy, prevents early ageing, promotes human body various functions extensive It is multiple.Raspberry invigorates the kidney and stops nocturnal emission establishing-Yang contracting urine in the present invention, nourishes the liver to improve visual acuity as monarch drug in a prescription.Sealwort, radix polygonati officinalis boosting qi and nourishing yin moistening lung, kidney-nourishing are good for Spleen, promote the production of body fluid to quench thirst as ministerial drug.Spina date seed nourishing heart tonifying liver, antitoxic heart-soothing and sedative；Matrimony vine is nourishing liver and kidney, benefiting shrewd head；Jujube tonifying middle-Jiao and Qi, Nourishing blood and tranquilization, three are adjutant altogether.Complete square all medicines share, plays kidney and spleen invigorating, nourishing heart tonifying liver, QI invigorating righting is gone with eliminating evil, heresy altogether Just from peace and disease amelioration or even to more.The document report of identical with the present invention prescription and its preparation is there is no at present.
The content of the invention
The present invention is in order to overcome the shortcomings of the prior art, there is provided a kind of preparation for being used to alleviate the preparation of physical fatigue Method, there is the significant effect for alleviating physical fatigue using preparation made from the inventive method, being capable of enriching yin and nourishing kidney, QI invigorating town Bitterly, keep or strengthen ovarian function, most crowds can be widely used in.
The purpose of the present invention is achieved by the following technical solution：A kind of preparation for being used to alleviate the preparation of physical fatigue Method, the preparation method specifically comprise the following steps：
A, raspberry, sealwort, radix polygonati officinalis, spina date seed, matrimony vine and jujube are taken by prescription portion rate, added water to cook 3 times, each 1h, made Obtain extract solution；
B, obtained extract solution in above-mentioned steps a is collected, filtration, the extract solution of 3 times is merged, it is dense to concentrate the filtrate to needs Degree, is cooled to room temperature；
C, using the extracting method of water extraction and alcohol precipitation method, it is slowly added to ethanol that concentration is 95% and quickly stirs, make containing for extract solution Alcohol amount is 40~70%, and preferable alcohol content is 50%, and 12h is placed in sealing, centrifuges to obtain supernatant, reclaims ethanol；
D, supernatant is collected, through being dried under reduced pressure at a temperature of 60 DEG C to dry cream, plant extracts is made after crushing；
E, particle product or powder is made in plant extracts.
The preparation method of particle product is in preparation：Obtained plant extracts in above-mentioned steps d is taken, adds additives, Mix, softwood is made with the ethanol that concentration is 80%, crosses 10 mesh medicines sieve, is dried at a temperature of 60 DEG C, whole grain, packing；Whole grain institute Medicine sieve is 10 mesh sieves and 65 mesh sieves.
The preparation method of powder is in preparation：Preparation method in step d is changed to collect supernatant, adds additives, Constant volume is filtered, dispensed, sterilize into the volume of needs；Sterilizing methods are gone out using boiling sterilization, flowing steam sterilization method or hot pressing Bacterium method.
Mixed in the step a by the raw material of following parts by weight：1~10 part of raspberry, 1~8 part of sealwort, radix polygonati officinalis 1 0~3 part of~8 parts, 1~6 part of spina date seed, 0~3 part of matrimony vine and jujube.Preferably, 4~8 parts of raspberry, 3~5 parts of sealwort, radix polygonati officinalis 1~2 part of 3~5 parts, 2~4 parts of spina date seed, 1~2 part of matrimony vine and jujube；Most preferably, 6 parts of raspberry, 4 parts of sealwort, radix polygonati officinalis 4 2 parts of part, 3 parts of spina date seed, 2 parts of matrimony vine and jujube.
In the step a, it is 8~12 times of raw material gross weight to decoct amount of water for the first time, after twice amount of water be original Expect gross weight 6~10 times.Preferably, it is 12 times of raw material gross weight to decoct amount of water for the first time, after twice amount of water be original Expect gross weight 10 times.
Additives are any one in the mixture of dietary supplement, filler, preservative, preservative and flavouring Kind or a variety of mixtures mixed with arbitrary proportion, the filler are the auxiliary material for meeting Chinese food laws and regulations requirement.
Dietary supplement is mineral matter, vitamin, natto, any one or more in protein powder arbitrarily to compare The mixture that example mixes；The mineral matter is selenium, zinc, calcium, magnesium, potassium, iron, chromium, any one or more in manganese to appoint The mixture that meaning ratio mixes；The vitamin is beta carotene, vitamin B1, vitamin B6, vitamin C, vitamin D, the mixture that any one or more in vitamin E is mixed with arbitrary proportion；The protein powder separates for soybean Any one or more mixture mixed with arbitrary proportion in albumen, lactalbumin, pea protein, collagen.
Raspberry：It is sweet can help, acid can restrain, warm-natured without scorching, kidney-replenishing and the disadvantage without impairment of yin, prevent prolapse and without sluggish Evil, and mend can be solid, beneficial to the work(of lower envelope Tibetans, be apt to control the card of kidney deficiency not admittedly.Representative medicine of the raspberry as the establishing-Yang that invigorates the kidney and stops nocturnal emission, Clinically it is usually used in treating neurasthenia, emission and spermatorrhea, premature ejaculation impotence, enuresis frequent micturition, consumptive disease, mesh is secretly dim-sighted to wait card.Pharmacology It is demonstrated experimentally that raspberry has anti-mutagenesis, anti-inflammatory is anti-oxidant, improving studing ability, anti-aging, strengthens immune wait and makees With.
Sealwort：It is sweet, put down.Returns spleen, lung, kidney channel.With boosting qi and nourishing yin, invigorating the spleen, moistening lung, the effect of kidney-nourishing.For treating spleen Stomach Qi deficiency, fatigue and asthenia, deficiency of stomach-Yin, dry deficiency of food, deficiency syndrome of the lung cough caused by dryness, overstrain cough hemoptysis, asthenia of essence and blood, soreness and weakness of waist and knees, beard and hair are early In vain, Heat Diabetes etc. is demonstrate,proved, and is one of conventional nourishing medicine of the traditional Chinese medical science.Modern study shows that sealwort has resisting pathogenic microbes（Carefully Bacterium, fungi, virus etc.）, reducing blood lipid is hypoglycemic, hypotensive, and anti-aging is antitumor, antifatigue, radioresistance, resist oxygen lack, antioxygen Change activity, enhancing is immune, and leucocyte caused by antagonism endoxan declines, strong neutrophil phagocytosis, expands coronal dynamic The effect such as arteries and veins, increase coronary blood flow.
Radix polygonati officinalis：《Sheng Nong's herbal classic》It is classified as top grade.It is sweet, it is slightly cold.Attach to the lung and stomach meridians.With nourishing yin to moisten dryness, the work(to promote the production of body fluid to quench thirst Effect.For treating the cards such as lung stomach-Yin wound, cough caused by dryness-heat, dry throat and mouth, Heat Diabetes.Modern study shows that radix polygonati officinalis, which has, improves Immunity, cardiac stimulant is hypoglycemic, reducing blood lipid, extends the effect such as animal lifespan.
Spina date seed:《Compendium of Materia Medica》It is classified as top grade.It is sweet, sour, put down.Return liver, courage, the heart channel of Hang-Shaoyin.With nourishing heart tonifying liver, calming heart Calm the nerves, arrest sweating, the effect of promoting the production of body fluid.For treating the card such as restlessness of asrhenia type and insomnia, horrified to much dream, body void hidrosis, injury thirst.Modern study Show, spina date seed has tranquilizing soporific, and analgesia, anticonvulsion, reducing blood lipid, platelet aggregation-against, enhancing is immune, hypotensive, resists and lacks Oxygen, resist myocardial ischemia, anti-arrhythmia cordis, anti-aging, the effect such as radioresistance.
Matrimony vine：First recorded in《Sheng Nong's herbal classic》It is listed in top grade.It is sweet, put down.Return liver and kidney channel.With nourishing liver and kidney, strengthening the essence is bright Purpose effect.For treating consumption consumptive loss, soreness of waist and knee joint, dizziness and tinnitus, impotence and seminal emission, Heat Diabetes, blood deficiency chlorosis, blurred vision is not It is bright to wait card.
Jujube：First recorded in《Sheng Nong's herbal classic》It is listed in top grade.It is sweet, temperature.Returns spleen, stomach, the heart channel of Hang-Shaoyin.With tonifying middle-Jiao and Qi, support The effect of blood and tranquilizing mind.For treating the card such as spleen eating less, weak loose stool, hysteria of womam.
Particle product provided by the invention, powder, comprising pharmaceutically acceptable additives, including it is filler, anti- In rotten agent, dietary supplement any one, the mixture that is mixed with arbitrary proportion of two kinds and the above.Wherein fill Agent is to meet the auxiliary material of Chinese food laws and regulations requirement, include but are not limited to ethanol, dextrin, starch, sucrose, glucose, honey, Mannitol, xylitol, Steviosin, lactose, fructose, protein sugar, maltitol, Aspartame, essence for food spices, citron It acid, can be used alone, also can be combined and use.
Preservative is the conventional auxiliary material for meeting national Specification, includes but are not limited to peppermint oil, cinnaldehydrum, folium eucalypti Oil, benzoic acid, sodium benzoate, methyl hydroxybenzoate, ethylparaben, propylben, butyl hydroxybenzoate, sorbic acid, can be independent Use, also can be combined and use.
The functional food of the present invention, it is to be processed by the raw material for forming above-mentioned formula by extraction or other modes, system Into intermediate raw material, it is necessary to when add pharmaceutically acceptable additives, be made according to the routine techniques of galenic pharmacy.Described centre Raw material can be obtained by extracting raw material respectively, can also be obtained by extracting raw material jointly, can also be obtained by other means The methods of arriving, carrying, chromatograph such as crushing, squeezing, grinding, sieving, diacolation, extraction, water extraction, alcohol extracting, ketone obtains, these intermediate raw materials Can be medicinal extract form, can be dry extract or liquid extract, according to preparation it is different need to determine to be made it is different dense Degree.
The present invention is using the extract mixtures of raspberry, sealwort, radix polygonati officinalis, spina date seed, matrimony vine and jujube as raw material, system Into particle product or powder.Particle product or powder have and alleviate physical fatigue according to Basic Theories of Chinese Medicine prescription, Improve energy healthcare function, can enriching yin and nourishing kidney, QI invigorating analgesia, keep or enhancing ovarian function.The present invention draws to many reasons The fatigue and sub-health population risen has obvious antifatigue effect, no obvious adverse reaction, no dependence；Simple process is easy OK, production cost is controllable, is advantageous to industrialized production, and a variety of formulations can be made as needed.
The present invention also has very big purposes in terms of physical fatigue is alleviated, improve energy and anti-aging.With chronic tired The most liver kidney deficiency of the crowd of labor syndrome or long labor prolonged illness is weak, insufficiency of vital energy and blood, preferably combine invigorate the kidney and stop nocturnal emission, Yangyin Rougan liver, tonifying Qi live Blood Chinese medicine.The health food presses traditional Chinese medical theory scientific composition, has no side effect safely.We are using raspberry as monarch drug in a prescription, kidney tonifying again Gas, controlling nocturnal emission with astringent drugs support marrow, with dispel it is tired refresh oneself, treatment kidney wound fatigue card.Minister is with the sealwort of boosting qi and nourishing yin kidney-nourishing, jade that nourishing Yin and promoting production of body fluid is moisturized Bamboo, play tonifying Qi enriching yin, the work(of moistening lung invigorating the spleen altogether, with dispel it is tired repose, treatment spleen and lung wound fatigue card.Assistant spina date seed nourishing heart tonifying liver, Antitoxic heart-soothing and sedative, jujube tonifying middle-Jiao and Qi, nourishing blood and tranquilization, with the tired benefit god that dispels, treat grieved fatigue card；Spina date seed is nourishing liver and kidney, strengthening the essence Improving eyesight, with tired inducing resuscitation of dispelling, treatment liver wound fatigue card.All medicines share, benefit profit is proper, and gas fills arteries and veins and answered, and internal organs reconcile, and fatigue self solves.
The beneficial effects of the invention are as follows：Given efficacy and pharmaceutical value of the invention based on above-mentioned natural plants, with certainly Right plant, traditional health-preserving method is combined with theory of medicine, is conceived to and alleviates physical fatigue, improve energy, anti-aging Deng by 6 kinds of raspberry, sealwort, radix polygonati officinalis, spina date seed, matrimony vine and jujube medicinal and edible plant optimization compoundings.On the one hand, qi-restoratives is solid Member, body is nourished, empty to mend, canonical is prosperous, and fatigue is removed certainly；On the other hand, tonifying kidney and strengthening yang, flourish muscle support marrow, and brain has been supported, and bone obtains Strong, fatigue can disappear.The present invention has further the advantage that：It is in good taste, safely and effectively, it is adapted to most of crowds to drink for a long time；Raw material Integration of drinking and medicinal herbs Chinese medicine or natural food are all derived from, safely and effectively, no ill-effect, nutrition is comprehensive.
Brief description of the drawings
Fig. 1 is schematic diagram of the particle product of the present invention to the analgesic activity of hot plate method induced pain mouse；
Fig. 2 is schematic diagram of the particle product of the present invention to the analgesic activity of glacial acetic acid induced pain mouse；
Fig. 3 is the schematic diagram of influence of the particle product of the present invention to mice auricle swelling；
Fig. 4 is the linear dependence figure between absorbance A value and Evans blue seepage discharge of the present invention；
Fig. 5 is the schematic diagram of influence of the particle product of the present invention to mouse capillary permeability；
Fig. 6 is the schematic diagram of influence of the particle product of the present invention to mouse toes swelling；
Fig. 7 is the schematic diagram of influence of the particle product of the present invention to mouse swimming time；
Fig. 8 is schematic diagram of the particle product of the present invention to the influence of keratinocyte on vagina；
Fig. 9 is the linear dependence figure between absorbance OD values of the present invention and progesterone P amounts (ng/mL)；
Figure 10 is the schematic diagram of influence of the particle product of the present invention to female mice progesterone P content；
Figure 11 is the schematic diagram of influence of the particle product of the present invention to female mice body weight, uterus and ovary weight and organ index；
Figure 12 is uterus sections micrograph of the present invention, and HE dyes × 2 times；
Wherein：A, blank group；B, particle product low dose group；C, particle product middle dose group；D, particle product high dose group；e、 Bolus for woman diseases group；
Figure 13 is ovarian sections micrograph of the present invention；
Wherein：A, the full cyclogram of ovarian sections（× 1 times）；B, blank group（× 10 times）；C, particle product low dose group（×10 Times）；D, particle product middle dose group（× 10 times）；E, particle product high dose group（× 10 times）；F, bolus for woman diseases group（× 10 times）； G, ovarian follicle form（× 40 times）；
Figure 14 is the schematic diagram of influence of the particle product of the present invention to female mice Follicles sum and corpus luteum number.
Embodiment
Below in conjunction with the drawings and specific embodiments, the invention will be further described.
A kind of preparation method for being used to alleviate the preparation of physical fatigue, the preparation method specifically comprise the following steps：
A, raspberry, sealwort, radix polygonati officinalis, spina date seed, matrimony vine and jujube are taken by prescription portion rate, added water to cook 3 times, each 1h, made Obtain extract solution；
B, obtained extract solution in above-mentioned steps a is collected, filtration, the extract solution of 3 times is merged, it is dense to concentrate the filtrate to needs Degree, is cooled to room temperature；
C, using the extracting method of water extraction and alcohol precipitation method, it is slowly added to ethanol that concentration is 95% and quickly stirs, make containing for extract solution Alcohol amount is 40~70%, and preferable alcohol content is 50%, and 12h is placed in sealing, centrifuges to obtain supernatant, reclaims ethanol；
D, supernatant is collected, through being dried under reduced pressure at a temperature of 60 DEG C to dry cream, plant extracts is made after crushing；
E, particle product or powder is made in plant extracts.
The preparation method of particle product is in preparation：Obtained plant extracts in above-mentioned steps d is taken, adds additives, Mix, softwood is made with the ethanol that concentration is 80%, crosses 10 mesh medicines sieve, is dried at a temperature of 60 DEG C, whole grain, packing；Whole grain institute Medicine sieve is 10 mesh sieves and 65 mesh sieves.
The preparation method of powder is in preparation：Preparation method in step d is changed to collect supernatant, adds additives, Constant volume is filtered, dispensed, sterilize into the volume of needs；Sterilizing methods are gone out using boiling sterilization, flowing steam sterilization method or hot pressing Bacterium method.
Mixed in the step a by the raw material of following parts by weight：1~10 part of raspberry, 1~8 part of sealwort, radix polygonati officinalis 1 0~3 part of~8 parts, 1~6 part of spina date seed, 0~3 part of matrimony vine and jujube.Preferably, 4~8 parts of raspberry, 3~5 parts of sealwort, radix polygonati officinalis 1~2 part of 3~5 parts, 2~4 parts of spina date seed, 1~2 part of matrimony vine and jujube；Most preferably, 6 parts of raspberry, 4 parts of sealwort, radix polygonati officinalis 4 2 parts of part, 3 parts of spina date seed, 2 parts of matrimony vine and jujube.
In the step a, it is 8~12 times of raw material gross weight to decoct amount of water for the first time, after twice amount of water be original Expect gross weight 6~10 times.Preferably, it is 12 times of raw material gross weight to decoct amount of water for the first time, after twice amount of water be original Expect gross weight 10 times.
Embodiment 1：Powder
Formula：Raspberry 60g, sealwort 40g, radix polygonati officinalis 40g, spina date seed 30g, matrimony vine 10g, jujube 10g and sodium benzoate 0.6g.
Preparation method：Raspberry, sealwort, radix polygonati officinalis, spina date seed, matrimony vine, jujube are taken, adds water to cook 3 times, each 1h, first Secondary amount of water is 12 times of above-mentioned raw materials total amount, after twice amount of water be 10 times of above-mentioned raw materials total amount.Collect extract solution, filter Cross, extract solution three times is merged, filtrate is concentrated into finite concentration, is cooled to room temperature, is slowly added to 95% ethanol and quickly stirs Mix, it is 50% to make alcohol content, and 12h is placed in sealing, centrifuges to obtain supernatant, adds additives, and constant volume filters into certain volume.Point Dress.Sterilizing.
Embodiment 2：Particle product
Formula：Raspberry 60g, sealwort 40g, radix polygonati officinalis 40g, spina date seed 30g, matrimony vine 10g, jujube 10g, DEXTRIN g, sucrose 80g With 80% ethanol 10mL.
Preparation method：Raspberry, sealwort, radix polygonati officinalis, spina date seed, matrimony vine, jujube are taken, adds water to cook 3 times, each 1h, first Secondary amount of water is 12 times of above-mentioned raw materials total amount, after twice amount of water be 10 times of above-mentioned raw materials total amount.Collect extract solution, filter Cross, extract solution three times is merged, filtrate is concentrated into finite concentration, is cooled to room temperature, is slowly added to 95% ethanol and quickly stirs Mix, it is 50% to make alcohol content, and 12h is placed in sealing, centrifuges to obtain supernatant, is dried under reduced pressure to dry cream, crushes through 60 DEG C, is added additional Agent, mix, cross 80 mesh sieves (No. five sieves）, using dry granulation, 80% appropriate ethanol is uniformly sprayed into, is made that " hand is pinched agglomerating, refers to Pressure i.e. dissipate " softwood, then in a manner of extruding by 10 mesh medicines sieve, uniform particle is made, rapidly puts the particle being prepared into In 60 DEG C of baking oven, 3h is dried.Dry particle is taken, with 10 mesh（No.1 is sieved）With 65 mesh（No. four sieves）Carry out whole grain.Packing.
Embodiment 3：Powder
Formula：Raspberry 60g, sealwort 40g, radix polygonati officinalis 40g, spina date seed 30g, matrimony vine 20g, jujube 15g and ethylparaben 0.5g. Preparation method is the same as embodiment 1.
Embodiment 4：Particle product
Formula：Raspberry 60g, sealwort 40g, radix polygonati officinalis 40g, spina date seed 30g, matrimony vine 20g, jujube 15g, dextrin 90g, Steviosin 0.04g and 80% ethanol 10mL.Preparation method is the same as embodiment 2.
The alleviation physical fatigue of Chinese medicine preparation of the present invention, energy is improved, keep or strengthen ovarian function, QI invigorating analgesic work( Effect can be proved by following 4 kinds of pharmacodynamic experiments：Experiment I：The analgesic experiment of hot plate method and writhing method；Experiment II：Caused by dimethylbenzene xylene The anti-inflammatory of mice auricle swelling method, mice caused by dimethylbenzene xylene auricle edema method and the experiment of carrageenan induced mice pedal swelling is real Test；Experiment III：The anti-fatigue test of mouse swimming；Experiment IV：Ovulation induction is tested.
Experiment I：The analgesic experiment of hot plate method and writhing method.
1.1 laboratory samples and reagent
Raspberry（Guangzhou Kang Sheng pharmaceutcal corporation, Ltds, lot number：20160912）；Spina date seed（Guangzhou Kang Sheng pharmaceutcal corporation, Ltds, batch Number：20161005）；Sealwort（Guangzhou Kang Sheng pharmaceutcal corporation, Ltds, lot number：20161012）；Radix polygonati officinalis（The limited public affairs of Guangzhou health sage's medicine company Department, lot number 20160928）；Jujube（Guangzhou Kang Sheng pharmaceutcal corporation, Ltds, lot number：20161125）；Matrimony vine（Guangzhou health sage's medicine company has Limit company, lot number：20161103）Above medicinal material is accredited as certified products through Chinese medicine institute of Guangdong pharmaceutical university horse swan goose associate professor.It is fixed Female pellet（Shanxi Guang Yuyuan traditional Chinese medicines Co., Ltd, lot number：139170102）；Aspirin enteric coated tablet（The limited public affairs of Bayer medicines and health protection Department, lot number：BJ32400）；Qi and blood recovering capsule（Yunnan Baiyao group mountain of papers seven spends Co., Ltd, lot number：WEA1345）；Silver yellow Grain（Hengsheng Pharmaceutical Co., Ltd., Zhongshan City, lot number：20161007）；Progesterone P kits（Shanghai Tong Wei Industrial Co., Ltd.s）； NaCl（Tianjin Zhi Yuan chemical reagent Co., Ltd, lot number：20160710）；Dimethylbenzene（Tianjin richness space fine chemistry industry is limited Company, lot number：20151120）；Evans blue（Shanghai Ru Ji biotechnologies Development Co., Ltd, lot number：150722）；Irish moss Glue（Shanghai Mike's woods biochemical technology Co., Ltd, lot number：C10068972）；Methylene blue（The limited public affairs of Chinese medicines group chemical reagent Department, lot number：20151223）：4% paraformaldehyde fixer（Wuhan bio tech ltd of Google, lot number:20160314）；Ice Acetic acid（Tianjin all generations Chemical Co., Ltd., lot number：20140808）；Above reagent is that analysis is pure.
1.2 experimental animal
SPF level Kunming mouses, male and female dual-purpose, weight (20 scholar 2) g, provided by Nanfang Medical Univ's Experimental Animal Center, Production licence number SCXK（Guangdong）2016-0041, divide cage to feed according to experimental nature and sex, free choice feeding drinking-water, claim every other day Weigh sb..22~24 DEG C of room temperature, to humidity 52%~56%.
1.3 instruments and producer
RE-2000A type vacuum rotary evaporators（Shanghai Yarong Biochemical Instrument Plant）；SHE-D (III) type circulating water type vavuum pump （Yuhua Instrument Co., Ltd., Gongyi City）；202-2AB type Constant Temp. Ovens（The limited public affairs of Tianjin Stettlen instrument Department）；The mm card punch of diameter 8（Beijing Zhong Shidichuan developments in science and technology Co., Ltd）；KQ-300DE numerical control ultrasonic cleaning machines （Kunshan Ultrasonic Instruments Co., Ltd.）；The hole water-bath of HH-S6 constant temperature six（Community of Jin Tan County Jin Cheng Guo Sheng laboratory apparatus factory）；YLS- 6A intelligence hot-plate instruments（Shandong Academy of Medical Sciences's equipment station）；Ou Nuo shaking tables（Tianjin Ounuo Instrument Co., Ltd.）；8 holes The liquid relief volley of rifle fire（The emerging wound laboratory apparatus of big dragon（Beijing）Co., Ltd）；MNT-150 Mei Naite origin digital display callipers（Shanghai Mei Naite Industrial Co., Ltd.）；OLYMPUS CKX41 inversion type biomicroscopes（Beijing development in science and technology Co., Ltd of Zhong Yiguang sections）； The general ELIASAs of ELX800 (Bai Teng Instrument Ltd. of the U.S. (BioTek)).
The preparation of 1.4 samples
Body weight in terms of 60kg, mouse daily dose in terms of 9 times of people, the mouse dosage of positive control drug with 0.2mL/10g is counted, and the mouse dosage of tested particle product particle is in terms of 0.3mL/10g.The specific of sample is formulated as follows：
1. given the test agent is prepared：The particle product that Example 2 is prepared into, add physiological saline（50-60℃）Dissolving, if necessary It is ultrasonically treated, is configured to basic, normal, high three kinds of concentration（100、150、225mg：1mL）Suspension.Wherein, particle product 9 times of the dosage of middle concentration group equivalent to people's normal dose.
2. Yinhuang Particle sample（59.99mg:1mL）Preparation：Yinhuang Particle is ground in after powder with mortar, weighs 6g Add physiological saline to 100mL, prepared before use.
3. aspirin enteric coated tablet sample（4.5mg:1mL）Preparation：It is in powder that aspirin enteric coated tablet is ground with mortar It is last, weigh 0.45g and add physiological saline to be ultrasonically treated if necessary, prepared before use to 100mL.
4. qi and blood recovering capsule sample（13.5mg:1mL）Preparation：After qi and blood recovering capsule goes capsule shells, weigh 0.54g and add life Salt solution is managed to 40mL, is ultrasonically treated if necessary, prepared before use.
5. bolus for woman diseases sample（81mg:1mL）Preparation：Bolus for woman diseases is ground in after powder with mortar, 3.24g is weighed and adds （50-60℃）Physiological saline is ultrasonically treated, prepared before use to 40mL.
6. 0.8% acetum：Accurate measuring 0.8mL glacial acetic acid adds distilled water constant volume to face into 100mL volumetric flask With preceding preparation.
7. 0.5% Evans blue physiological saline：Weigh 0.5g Evans blue powder and be completely dissolved in 100mL physiological saline, pass through 0.22 μm of miillpore filter filtration, prepared before use.
8. 0.8% acetic acid physiological saline：Accurate measuring 0.8mL glacial acetic acid adds physiological saline constant volume to 100mL volumetric flask In, prepared before use.
9. 1% carrageenan suspension：24h weighs 50mg carrageenan powder and is dissolved in 50mL physiological saline before use, repeatedly Shaking is ultrasonically treated to without indissoluble thing gel, is placed in 4 DEG C of preservations if necessary.
10. 5% methylene blue solution：95% ethanol solution that 0.5g methylene blue powder is completely dissolved in 100mL is weighed, is matched somebody with somebody before use System.
Experiment I：The analgesic experiment of mouse.
2.1.1 hot plate method：
Take healthy Kunming kind female mice（18-22 g）, intelligent hot-plate instrument temperature is adjusted to（55±0.5）DEG C, preheat 10 minutes Afterwards, female mice 1 is merely placed on hot plate every time, the time required to mouse licks left back foot from putting to occurring（s）As the mouse Pain threshold, it is qualified to screen（The sufficient time is licked in 5s to 30s）Female mice 75 be only divided into 5 groups, every group 15 by table of random number Only, respectively physiological saline group（0.2 mL/10 g）, aspirin positive controls, particle product low dose group, middle dosage Group, high dose group.Replication pain threshold before every group of administration, take twice threshold of pain average value as normal pain threshold.Adaptability is fed After supporting 3 days, relative medicine gavage is carried out by different groups, 1 time a day, claims time body weight every other day, according to body weight by being only administered, continuously Administration 7 days.Respectively at the pain threshold of 30min, 60min, 90min measurement mouse after last dose, if painless anti-in mouse 60s Should, take out, calculated by 60s immediately, record result, calculate each group threshold of pain and improve percentage, as a result carried out with SPSS22 between group T is examined, with P<0.05 is statistically significant.
2.1.2 writhing method：
Take healthy Kunming mouse（18-22 g）75, male and female half and half, it is divided into 5 groups by table of random number, every group 15, distinguishes For physiological saline group（0.2 mL/10 g）, aspirin positive controls, particle product low dose group, middle dose group, high dose Group.After adaptability is fed 3 days, relative medicine gavage is carried out by different groups, 1 time a day, claims time body weight every other day, according to body weight by Only it is administered, successive administration 7 days.The 30min after last dose, each mouse are newly prepared by 0.1ml/10g dosage intraperitoneal injection 0.8% acetum, observe and record every mouse in 15min and writhing response occur（Belly shrinks indent, stretches hind leg, stern Portion raises, crawling）Number, calculate analgesia percentage, as a result carrying out t between group with SPSS22 examines, with P<0.05 is to have Statistical significance.Analgesia percentage (%)=（The average writhing number of the average writhing number-administration group of saline control group）/ Average writhing number × 100% of saline control group
Experiment II：The anti-inflammatory experiment of mouse.
Mice caused by dimethylbenzene xylene auricle edema method：
Take healthy Kunming mouse（18-22g）105, male and female half and half, it is divided into 7 groups by table of random number, every group 15, distinguishes Blank group（0.2 mL/10 g physiological saline）, model group（0.2 mL/10 g physiological saline）, Yinhuang Particle group, aspirin Group, particle product low dose group, middle dose group, high dose group.After adaptability is fed 3 days, using mice ear anti-inflammatory method, press Different groups carry out relative medicine gavages, 1 time a day, claim time body weight every other day, according to body weight by being only administered, successive administration 10 days. 0.5h after last dose, in addition to blank group, 0.02ml dimethylbenzene is uniformly applied to the left ear auricle two of every mouse by remaining each group Face causes inflammation, and auris dextra is not applied as normal control.Neck is taken off after 1h and puts to death each mouse, with diameter 8mm card punch in left ear and auris dextra Round auricle is laid at same position, and with scales/electronic balance weighing, auris dextra weight is subtracted as swelling using left ear weight, is calculated ear respectively and is swollen Expansibility and swelling inhibiting rate, t between group is as a result carried out with SPSS22 and examined, with P<0.05 is statistically significant.
Swelling inhibiting rate（%）=（The average swelling of the average swelling-administration group of model group）/（The average swelling of model group） ×100%
Mice caused by dimethylbenzene xylene auricle edema method mouse peritoneal capillary permeability is tested：
Take healthy Kunming mouse（18-22 g）90, male and female half and half, it is divided into 6 groups, every group 15 by table of random number.Respectively For normal group（The mL/10 g of physiological saline 0.2）, model group（The mL/10 g of physiological saline 0.2）, Yinhuang Particle group, particle product Basic, normal, high dosage group.After adaptability is fed 3 days, relative medicine gavage is carried out by different groups, 1 time a day, claims time body every other day Weight, according to body weight by being only administered, successive administration 10 days.Fasting 12h before last time is administered, after last dose 30min, each mouse The equal Evans blue physiological saline 0.1ml/10g of tail vein injection 0.5% (filters through miillpore filter）, belly is gently rubbed, abdominal cavity is noted immediately Penetrate 0.8% acetic acid physiological saline 0.2ml/10g（Normal group does not inject acetic acid as control）, neck is taken off after 30min and puts to death each mouse, Abdominal cavity is splitted, with 5ml normal saline flushing numbers, collects washing lotion 10mL, 3000r/min centrifugation 20min, takes supernatant, use is pure Water school zero, trap is determined at 590nm with ultraviolet specrophotometer（OD values）, t between group is as a result carried out with SPSS22 and examined Test, with P<0.05 is statistically significant.
In addition, azovan blue concentration, method are released according to absorbance A value：It is the two-fold dilution of 0.5% azovan blue solution And in colorimetric surveys absorbance A value at 590nm on spectrophotometer, it is known that concentration mensuration point replication 2 times, its average is as A Value, seeks the linear dependence between A values and seepage discharge (μ g/mL), obtains the other Evans blue seepage discharge of each group.Administration group presses down Rate processed（%）=（Model group absorbance values-administration group absorbance values）/ model group absorbance values × 100%.
Carrageenan induced mice pedal swelling is tested：
Take healthy Kunming mouse（18-22 g）105, male and female half and half, it is divided into 7 groups by table of random number, every group 15, distinguishes For blank group (mL/10 g of physiological saline 0.2), model group（The mL/10 g of physiological saline 0.2）, aspirin group, Yinhuang Particle Group, particle product low dose group, middle dose group and high dose group.After adaptability is fed 3 days, relative medicine is carried out by different groups Gavage, 1 time a day, claim time body weight every other day, according to body weight by being only administered, successive administration 10 days.Vernier calliper is first used after last dose Chi（Precision 0.02mm）The thickness of all mouse left hind vola pedis is surveyed, 1% carrageenan is then subcutaneously injected at left hind vola pedis Suspension（Every 0.02mL）, blank group injection normal saline, cause acute pedal swelling model.1% jiao of measure injection respectively 30min, 1h, 2h, 3h mouse cause the thickness of rear vola pedis after inflammation after fork dish glue suspension, so that sufficient before and after vola pedis thickness and cause are scorching after inflammation The difference of the thickness of plantar using swelling as Testing index, carries out statistical analysis, investigates medicine Carrageenan as swelling The influence of induced mice pedal swelling.Next day puts to death mouse after causing inflammation, takes the rear vola pedis of mouse or so, is precisely weighed, left and right vola pedis Difference is inflammatory swelling degree, calculates mouse pedal swelling inhibiting rate.As a result carry out t between group with SPSS22 to examine, with P<0.05 To be statistically significant.Swelling rate calculation formula： E(%)=（Volume-cause inflammation front volume after cause is scorching）The scorching front volume of/cause × 100%.Pedal swelling inhibiting rate（%）=（Left foot weight-right lumping weight amount）/ right lumping weight amount × 100%
Experiment III：The anti-fatigue test of mouse swimming.
Take healthy Kunming mouse（18-22 g）75, male and female half and half, it is divided into 5 groups by table of random number, every group 15, Respectively physiological saline group（0.2 mL/10 g）, qi and blood recovering capsule group, particle product low dose group, middle dose group, high dose Group.After adaptability is fed 3 days, relative medicine gavage is carried out by different groups, 1 time a day, claims time body weight every other day, according to body weight by Only it is administered, successive administration 10 days.It is administered after 1 h and mouse is put into depth of water 30cm, the swimming trunk that 25 DEG C of water temperature（Specification 72*53* 43cm）Went swimming, 8 every batch.Clean water is changed under per a collection of mouse before water, measures water temperature.Make in whole experiment process Every mouse four limbs keep motion, if mouse swims in the water surface, four limbs are motionless, are stirred with wooden stick in its near its circumference.Record small Mouse since swimming to the death time, the power as mice burden swimming exhausts the death time（When doing one's utmost, the death time is more than During 200min, in terms of 200min）.As a result carry out t between group with SPSS22 to examine, with P<0.05 is to have statistics meaning.
Experiment IV：The ovulation induction experiment of mouse.
Take the healthy teenage female mice of Kunming kind（18-22 g）75, random is 5 groups, every group 15, respectively physiology Salt solution group（0.2 mL/10 g）, bolus for woman diseases group, decoction particles product low dose group, middle dose group, high dose group are wanted well.Adaptability After feeding 5 days, relative medicine gavage is carried out by different groups, 1 time a day, claims time body weight every other day, according to body weight by being only administered, even Continuous administration 15 days（3-4 cycle）.Empirically require to carry out the investigation of indices, t between group is as a result carried out with SPSS22 and examined Test, with P<0.05 is statistically significant.
2.4.1 vagina superficial cell inspection：
After 10d is administered, daily at 9 points in the morning, continuously daily vaginal smear 1 time of each group mouse.By aseptic cotton carrier physiology salt Mouse vagina is inserted after water-soaked, takes out after fully dipping secretion, is gently rolled on slide, secretion is equably coated with In on slide, natural air drying, dyed with 5% methylene blue solution (preparation of 95% ethanol solution) newly configured, it is unnecessary to be gone after 2h with water drift Dyeing liquor, wait to do, be placed in the situation of biology microscope Microscopic observation vagina superficial cell, continuous 1 oestrous cycle of smear (5d).Be more than the smear gross area using superficial cell area 50% is used as positive criterion, calculating positive rate.
2.4.2 progesterone P puts inspection-free survey in serum：
After last dose 1h, each group mouse takes blood with plucking eyeball method, taken blood is put after 4 DEG C of environment stand 2h, centrifugation (3000 r/min, 10min), serum is separated, is operated according to kit specification.Determine the content of progesterone P in mice serum.It is pregnant Ketone P ELISAs（ELISA）Concrete operation step it is as follows:
1. the sample-adding of standard items：Setting standard sample wells and sample well, standard sample wells respectively add the μ L of standard items 50 of various concentrations.
2. it is loaded：Blank well is set respectively（Blank control wells are not added with sample and enzyme marking reagent, and remaining each step operation is identical）、 Testing sample hole.First add the μ L of sample diluting liquid 40 in testing sample hole on enzyme mark coating plate, then again plus with the μ L of test sample product 10 （The final dilution factor of sample is 5 times）.Sample is added on ELISA Plate bottom hole portion by sample-adding, is tried not to touch hole wall, is gently rocked mixed It is even.
It is 3. enzyme-added：Add the μ L of enzyme marking reagent 100 per the hole liquid relief volley of rifle fire, in addition to blank is empty.
4. incubate：With shrouding film shrouding, rearmounted 37 DEG C incubate 60 minutes.
5. match somebody with somebody liquid：It is standby after being diluted with 20 times of 20 times of concentrated cleaning solution distilled water.
6. wash：Shrouding film carefully is opened, discards liquid, is dried, cleaning solution is filled it up with per hole, is discarded after standing 30 seconds, such as This is repeated 5 times, and pats dry.
7. develop the color：Per Kong Xianjia developer A50 μ L, then add developer B50 μ L, gently concussion mixes, 37 DEG C of lucifuge colour developings 15 minutes.
8. terminate:Add the μ L of terminate liquid 50, terminating reaction per hole（Now blueness turns blueness immediately）.
9. determine：Returned to zero with blank well, 450nm wavelength sequentially measures the absorbance in each hole（OD）Value.Measure should add end Only carried out after liquid in 15 minutes.
10. calculate：Using the concentration of reference material as abscissa, OD values are abscissa, draw standard curve, draw linear regression Equation, corresponding concentration is calculated according to the OD values of sample, multiplied by with the actual concentrations of extension rate, as sample.
2.4.3 uterus, ovary weight and assessment of indices
Pluck after eyeball takes blood and rapidly put to death mouse, split abdominal cavity, take out uterus and both sides ovary, peel off surrounding tissue and fat Fat, rapidly with electronic balance weighing uterus and the weight in wet base of ovary.And it is converted into organ index:Uterus index=uterus weight (mg) / 100g body weight, ovarian index=ovary weight (mg)/100 g body weight.
2.4.4 uterus and ovary tissue morphological examination
The fresh uterus taken and ovary tissue sample are fixed with 4% paraformaldehyde fixer, conventional dehydration, waxdip embedding, cut Piece, HE dyeing.Biology microscope inspection and computer shooting, tissues observed morphological change, and record each in bilateral ovaries section Level ovarian follicle sum and corpus luteum number.
Experimental result and analysis
Fig. 1 is schematic diagram of the particle product to the analgesic activity of hot plate method induced pain mouse in an embodiment of the invention.
As shown in Figure 1,30min after administration, the pain threshold of the basic, normal, high dosage group of particle product improve percentage and physiology Salt solution group comparing difference is not statistically significant（P>0.05）.60min after administration, particle product low dose group, high dose group Pain threshold improves percentage and physiological saline group comparing difference is statistically significant（P<0.05）；90min after administration, particle product Middle dose group, the pain threshold of high dose group improves percentage and physiological saline group comparing difference is statistically significant（P<0.05 or P <0.01）, i.e., particle product can improve to some extent hot plate method pain model mouse pain threshold, reduce pain reaction number, its Analgesic effect has obvious dose-effect relationship, and the analgesic activity of low dose group is worst.（Note：Compared with physiological saline group：*P< 0.05, * *P<0.01；Compared with aspirin group：▲P<0.05, ▲▲P<0.01.）
Fig. 2 is schematic diagram of the particle product to the analgesic activity of glacial acetic acid induced pain mouse in an embodiment of the invention.
As shown in Figure 2, the basic, normal, high dosage group of particle product can significantly decrease the writhing number of acetic acid induced pain mouse, It is statistically significant with physiological saline group comparing difference（P<0.01）；The incubation period of writhing, particle can be extended to some extent Product high dose group and physiological saline group comparing difference are statistically significant（P<0.01）.In terms of analgesia rate, its analgesic effect tool There is obvious dose-effect relationship.（Note：Compared with physiological saline group：*P<0.05, * *P<0.01；Compared with aspirin group：▲P <0.05, ▲▲P<0.01.）
Fig. 3 is the schematic diagram of influence of the particle product to mice auricle swelling in an embodiment of the invention.
Swollen from the figure 3, it may be seen that the basic, normal, high dosage group of particle product can substantially suppress the Mice Auricle as caused by dimethylbenzene Swollen, anti-inflammatory effect and model group comparing difference are statistically significant（P<0.01）, but group difference unobvious（P>0.05）；Particle Product high dose group and aspirin group and Yinhuang Particle group comparing difference are not statistically significant（P>0.05）.In terms of inhibiting rate, Particle product is in certain dose-dependence to the inhibiting rate of auricle edema, and with the increase of dosage, inhibitory action has increased Trend.（Note：Compared with model group：*P<0.05, * *P<0.01；Compared with aspirin group：▲P<0.05, ▲▲P< 0.01；Compared with Yinhuang Particle group：▼P<0.05, ▼ ▼ P<0.01.）
Fig. 4-5 is the schematic diagram in an embodiment of the invention.Wherein, Fig. 4 absorbance As value and Evans blue seepage discharge (μ G/mL the linear dependence figure between), the schematic diagram of influence of Fig. 5 particle products to mouse capillary permeability.
As shown in Figure 5, the basic, normal, high dosage group of particle product can reduce mouse capillary permeability, reduce peritoneal fluid Body oozes out, and has obvious antiinflammatory action, anti-inflammatory effect and model group comparing difference are statistically significant（P<0.01）.Particle Product middle dose group, high dose group and Yinhuang Particle group comparing difference are not statistically significant（P>0.05）.In terms of inhibiting rate, particle The inhibiting rate of product low dose group is worst, but does not have obvious dose-effect relationship between low middle high dose group.（Note：With blank group ratio Compared with：*P<0.05, * *P<0.01；Compared with Yinhuang Particle group：▲P<0.05, ▲▲P<0.01；Compared with model group：▼P< 0.05, ▼ ▼ P<0.01.）
Fig. 6 is the schematic diagram of influence of the particle product to mouse toes swelling in an embodiment of the invention.
It will be appreciated from fig. 6 that injection carrageenan causes 30min after inflammation, particle product middle dose group, high dose group Carrageenan Caused normal mouse toes swelling has inhibitory action, and anti-inflammatory effect and model group comparing difference are statistically significant（P<0.01）； 2h, 3h after cause is scorching, particle product high dose group and model group comparing difference are statistically significant（P<0.05）；24h after cause is scorching, The grain basic, normal, high dosage group of product and model group comparing difference are not statistically significant（P>0.05）, have with blank group comparing difference Statistical significance（P<0.05 or P<0.01）, i.e., the inhibitory action of normal mouse toes swelling caused by particle product Carrageenan Unobvious.（Note：Compared with blank group：*P<0.05, * *P<0.01；Compared with model group：▲P<0.05, ▲▲P< 0.01.）
Fig. 7 is the schematic diagram of influence of the particle product to mouse swimming time in an embodiment of the invention.
As shown in Figure 7, the basic, normal, high dosage group of particle product can be obviously prolonged mouse swimming power exhaust the death time, resist Fatigue effect and blank group comparing difference are statistically significant（P<0.01）, and be in obvious dose-effect relationship between group, with administration Dosage increase, antifatigue significant effect enhancing；The anti-fatigue effect of particle product is better than qi and blood recovering capsule group, middle dose group, height Dosage group and qi and blood recovering capsule group comparing difference are statistically significant（P <0.01）.（Note：Compared with physiological saline group：*P< 0.05, * *P<0.01；Compared with qi and blood recovering capsule group：▲P<0.05, ▲▲P<0.01.）
Fig. 8-14 is the schematic diagram in an embodiment of the invention.Wherein, Fig. 8 is particle product to angling on female mice vagina The schematic diagram of the influence of cell；Fig. 9 is the linear dependence figure between absorbance OD values and progesterone P amounts (ng/mL)；Figure 10 is The schematic diagram of influence of the grain product to female mice progesterone P content；Figure 11 be particle product to female mice body weight, uterus and ovary weight and The schematic diagram of the influence of organ index；Figure 12 is the schematic diagram of the uterus HE sections of female mice；Figure 13 is the ovary HE sections of female mice Schematic diagram；Figure 14 is the schematic diagram of influence of the particle product to female mice Follicles sum and corpus luteum number.
Particle product is as follows to the influence result of keratinocyte on female mice vagina：
Through smear microexamination, the regular change of superficial cell quantity and quantity of leucocyte in inverse ratio can be observed in blank group Change, the cyclically-varying of obvious preoestrus, estrus, metaoestrus and anoestrum is presented.Particle product group and the positive Control group can be observed occur in blocks or agglomerate superficial cell on vaginal smear within the sampling time, and only a small amount of is white Cell, showing as vaginal exfoliated sexual cycle extends, is irregular.As shown in Figure 8, bolus for woman diseases group, particle product middle dose group The estrus of female mice can be extended to some extent with high dose group, positive rate of the superficial cell more than 50% and sky White group compares, and difference is statistically significant（P <0.05 or P<0.01）.Bolus for woman diseases group and particle product high dose group are poor It is different not statistically significant（P >0.05）.（Note：Compared with blank group：*P<0.05, * *P<0.01；Compared with bolus for woman diseases group：▲ P<0.05, ▲▲P<0.01.）
Particle product is as follows to the influence result of female mice progesterone P content：
As shown in Table 10, the basic, normal, high dosage group of particle product can be raised in mice serum to some extent compared with blank group Progesterone P content；For particle product high dose group compared with blank group, difference is statistically significant（P <0.01）.Particle product raises The effect of progesterone P content is better than bolus for woman diseases group, and for high dose group compared with female red group, difference is statistically significant（P <0.01）.
Influence result of the particle product to female mice body weight, weight of reproductive organs and organ index is as follows：
As shown in Figure 11, compared with blank group, the basic, normal, high dosage group body weight of particle product is without significant change, and uterus weight is Increase, uterus index is slightly larger, but difference is not statistically significant（P>0.05）；Particle product high dose group compared with blank group, Ovary weight substantially increases, and ovarian index significantly increases, and difference is statistically significant（P<0.01）.Compared with bolus for woman diseases group, The equal no significant difference of mouse weight, uterus weight, uterus index of the grain basic, normal, high dosage group of product（P>0.05）；Particle product In, low dose group compared with bolus for woman diseases group, ovary weight is less than normal, and ovarian index is relatively low, and difference is statistically significant（P<0.01）； Particle product high dose group and bolus for woman diseases group ratio, both ovary weights and the equal no significant difference of ovarian index（P>0.05）, i.e., it is high The particle product of dosage can increase the ovary weight and ovarian index of mouse.
Particle product is as follows to the influence result of uterus and ovarian morphology：
From Figure 12, Figure 13, blank group uterine cavity intimal epithelium thinner thickness, lumen of gland is slightly expanded, and blood vessel is less；Ovary body Product is slightly smaller, and color and luster is pale, and ovarian follicle structure is unclear, or vesica is in reset condition.The ovum of the visible different developmental phases in ovary cortex portion Bubble, it is seen that a small amount of corpus luteum and lean type are formed, and have no folliculi ovarici vesiculosi, it is seen that appropriate fat drips.Compared with blank group, particle product is low, Middle and high dosage group uterus outward appearance is thick blue, and inner membrane of uterine cavity epithelium is thickening, lumen of gland expansion, it is seen that vacuole under core, interstitial are loose； Ovary color and luster is more bright-coloured, and ovarian follicle significantly increases, and ovarian follicle has no obvious cystic dilatation, cytomorphosis, downright bad cell infiltration etc. Lesion；Medullary substance has no congested, oedema and cell infiltration.More corpus luteum is seen on surface, and the granular cell of corpus luteum physically well develops, form Completely, marshalling is compacted, and illustrates that ovary has been ovulated.
Particle product is as follows to the influence result of Follicles sum and corpus luteum number in ovary：
As shown in Figure 14, compared with blank group, trend that the ovarian follicle sum of the basic, normal, high dosage group of particle product is significantly increased, But both no significant differences（P>0.05）.Compared with blank, particle product low dose group, the corpus luteum number of middle dose group It increased, but difference no significant difference（P>0.05）, the corpus luteum number of the high agent group of particle product substantially increases, and difference has statistics Learn meaning（P<0.05）.
The present invention in the effect experiment stage, investigated in embodiment 2 particle product to mouse corrosion disease analgesia, it is antifatigue, promote The influence of Effect of Ovulation, the results showed that the present invention has certain anti-inflammatory and analgesic effect, has significant anti-fatigue effect, one Determine that progesterone P content can be raised in degree, promote uterus and the development of ovary, keep or strengthen the function of reproductive organs, promote ovarian follicle Development and ovulation.In a word, can be proved by above-mentioned various experiments, Chinese medicine and health food of the invention, which has, alleviates physical fatigue Healthcare function, can enriching yin and nourishing kidney, QI invigorating analgesia, keep or enhancing ovarian function.
Finally it should be noted that above content is merely illustrative of the technical solution of the present invention, rather than the present invention is protected The limitation of scope, the simple modification or equivalent substitution that one of ordinary skill in the art is carried out to technical scheme, All without departing from the spirit and scope of technical solution of the present invention.

Claims (10)

  1. A kind of 1. preparation method for being used to alleviate the preparation of physical fatigue, it is characterised in that：It is described to be used to alleviate physical fatigue The preparation method of preparation specifically comprises the following steps：
    A, raspberry, sealwort, radix polygonati officinalis, spina date seed, matrimony vine and jujube are taken by prescription portion rate, added water to cook 3 times, each 1h, made Obtain extract solution；
    B, obtained extract solution in above-mentioned steps a is collected, filtration, the extract solution of 3 times is merged, it is dense to concentrate the filtrate to needs Degree, is cooled to room temperature；
    C, using the extracting method of water extraction and alcohol precipitation method, it is slowly added to ethanol that concentration is 95% and quickly stirs, make containing for extract solution Alcohol amount is 40~70%, and 12h is placed in sealing, centrifuges to obtain supernatant, reclaims ethanol；
    D, supernatant is collected, through being dried under reduced pressure at a temperature of 60 DEG C to dry cream, plant extracts is made after crushing；
    E, particle product or powder is made in plant extracts.
  2. 2. the preparation method according to claim 1 for being used to alleviate the preparation of physical fatigue, it is characterised in that：In preparation Grain product preparation method be：Obtained plant extracts in above-mentioned steps d is taken, adds additives, is mixed, is 80% with concentration Ethanol be made softwood, cross 10 mesh medicines sieve, dried at a temperature of 60 DEG C, whole grain, packing；Medicine sieve used in whole grain is 10 mesh sieves With 65 mesh sieves.
  3. 3. the preparation method according to claim 1 for being used to alleviate the preparation of physical fatigue, it is characterised in that：Powder in preparation The preparation method of shape product is：Preparation method in step d is changed to collect supernatant, adds additives, constant volume is into needs Volume, filter, dispense, sterilizing.
  4. 4. the preparation method for being used to alleviate the preparation of physical fatigue according to claim 1,2 or 3, it is characterised in that：Its It is characterised by：Mixed in the step a by the raw material of following parts by weight：1~10 part of raspberry, 1~8 part of sealwort, radix polygonati officinalis 1 0~3 part of~8 parts, 1~6 part of spina date seed, 0~3 part of matrimony vine and jujube.
  5. 5. the preparation method for being used to alleviate the preparation of physical fatigue according to claim 1,2 or 3, it is characterised in that： In the step a, it is 8~12 times of raw material gross weight to decoct amount of water for the first time, after twice amount of water be the 6 of raw material gross weight ~10 times.
  6. 6. the preparation method according to claim 5 for being used to alleviate the preparation of physical fatigue, it is characterised in that：In the step In rapid a, decoct that amount of water is raw material gross weight for the first time 12 times, after twice amount of water be 10 times of raw material gross weight.
  7. 7. the preparation method for being used to alleviate the preparation of physical fatigue according to Claims 2 or 3, it is characterised in that：It is additional Agent is any one or more in the mixture of dietary supplement, filler, preservative, preservative and flavouring to appoint The mixture that meaning ratio mixes, the filler are the auxiliary material for meeting Chinese food laws and regulations requirement.
  8. 8. the preparation method according to claim 7 for being used to alleviate the preparation of physical fatigue, it is characterised in that：Described meals Food nutritious supplementary pharmaceutical is that mineral matter, vitamin, natto, any one or more in protein powder are mixed with arbitrary proportion Mixture；The mineral matter is that selenium, zinc, calcium, magnesium, potassium, iron, chromium, any one or more in manganese are mixed with arbitrary proportion The mixture formed；The vitamin is beta carotene, vitamin B1, vitamin B6, vitamin C, vitamin D, vitamin E In any one or more mixture mixed with arbitrary proportion；The protein powder is soybean protein isolate, whey Any one or more mixture mixed with arbitrary proportion in albumen, pea protein, collagen.
  9. 9. the preparation method according to claim 3 for being used to alleviate the preparation of physical fatigue, it is characterised in that：The sterilizing Method uses boiling sterilization, flowing steam sterilization method or autoclaving.
  10. 10. the preparation method according to claim 1 for being used to alleviate the preparation of physical fatigue, it is characterised in that：In step c The alcohol content for making extract solution is 50%.
CN201711114016.5A 2017-11-13 2017-11-13 A kind of preparation method for being used to alleviate the preparation of physical fatigue Pending CN107853703A (en)

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Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN105288324A (en) * 2015-12-10 2016-02-03 辽宁中医药大学 Medicine-food formula for constitutions with Yin-deficiency
CN106074818A (en) * 2016-08-04 2016-11-09 广禾堂草本生物科技（上海）有限公司 There is health food of promotion lactogenic effect and preparation method thereof
CN106309560A (en) * 2016-10-10 2017-01-11 扬中牧乐药业有限公司 Preparation method of compound mulberry leaf/folium cortex eucommiae granules
CN106880784A (en) * 2017-03-23 2017-06-23 庞会心 It is a kind of with fatigue-relieving, the Chinese medicine composition of anti-aging and its application

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN105288324A (en) * 2015-12-10 2016-02-03 辽宁中医药大学 Medicine-food formula for constitutions with Yin-deficiency
CN106074818A (en) * 2016-08-04 2016-11-09 广禾堂草本生物科技（上海）有限公司 There is health food of promotion lactogenic effect and preparation method thereof
CN106309560A (en) * 2016-10-10 2017-01-11 扬中牧乐药业有限公司 Preparation method of compound mulberry leaf/folium cortex eucommiae granules
CN106880784A (en) * 2017-03-23 2017-06-23 庞会心 It is a kind of with fatigue-relieving, the Chinese medicine composition of anti-aging and its application

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
作者不详: "产后腰酸腰痛的五大元凶，你造么？", 《39健康网》 *
张宏霞: "抗疲劳药食两用中药现状分析", 《湖南中医药大学学报》 *

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Application publication date: 20180330