CN107837208B

CN107837208B - Preparation method of telomerase composition and application of telomerase composition in anti-aging and anti-retrogrowth cosmetics

Info

Publication number: CN107837208B
Application number: CN201711173294.8A
Authority: CN
Inventors: 秦松辉
Original assignee: Shanghai Lianheng Biotechnology Co ltd
Current assignee: Shanghai Lianheng Biotechnology Co ltd
Priority date: 2017-08-17
Filing date: 2017-11-22
Publication date: 2021-02-02
Anticipated expiration: 2037-11-22
Also published as: CN107837208A

Abstract

The invention discloses a telomerase composition, a preparation method and application thereof in anti-aging and anti-retrogradation cosmetics. The telomerase composition is prepared by adding a broccoli telomerase extract, a pinus tabulaeformis leaf telomerase extract, a ginkgo leaf telomerase extract and a telomerase activator. The method also provides an anti-aging and anti-retrogressive cosmetic added with the telomerase composition and a preparation method thereof. Compared with the prior art, the telomerase composition and the anti-aging and anti-retrogrowth cosmetics prepared by the invention have excellent anti-aging and anti-retrogrowth performances.

Description

Preparation method of telomerase composition and application of telomerase composition in anti-aging and anti-retrogrowth cosmetics

Technical Field

The invention relates to the technical field of telomerase compositions, and in particular relates to an application of a telomerase composition in an anti-aging and anti-retrogrowth cosmetic.

Background

The 2009 nobel prize winning study revealed the root cause of aging, and one cannot see the genetic structure called telomere by scientists, which is the answer to the aging puzzle. Telomeres consist of repetitive DNA sequences, similar to the protective caps at the microdots of the shoelace, forming a cap at the end of the chromosome, protecting our DNA. When humans are born, telomeres are long. Over time, telomeres begin to shorten. Telomeres shorten due to cell division, opening the cell senescence cycle. When telomeres become too short, the cells have no ability to perform their function. Begin to self-destruct and eventually die. Scientists refer to apoptosis (apoptosis). Apoptosis is a matter of course, but when cells cease to function, large quantities of free radicals and molecules that cause inflammation are excreted, damaging nearby normal cells. It was found that telomere structure, which is the complete and important role of the shoulder protection gene, requires a specific substance, the enzyme, to maintain. The telomere length is maintained to protect the gene and thus to maintain human life in a healthy state. This particular enzyme, which is a combination of three specific proteins, is designated telomerase. Thousands of studies have shown an absolute correlation between telomere shortening and cellular senescence.

Telomerase is a ribonucleoprotein that catalyzes the increase of telomeric repeats at the end of the telomere. Telomeres are long sequences of repetitive sequences that can cap the ends of chromosomes and are thought to stabilize chromosomes.

Telomerase is not expressed in most adult cells and with a continuous cycle of replication, telomere length decreases. After a certain number of replication cycles, progressive telomere shortening can cause cells to enter a critical phase of telomeres, which in turn can lead to senescence of the cells. Certain diseases are associated with rapid telomere shortening, leading to premature cell senescence. In contrast to cancer cells and certain stem cells, somatic cells have little telomerase activity and stop dividing when the telomeric ends of at least some chromosomes decrease to a critical length, resulting in programmed cell death. Since the loss of telomeric repeats that cause senescence in somatic cells is increased by lower telomerase activity, induction of telomerase activity can confer increased replicative capacity on dying cells, proliferative capacity on senescent cells and appropriate repair of damaged tissues out of the cell cycle. It follows that activation and maintenance of telomerase activity plays an important role in combating cellular senescence.

Telomerase extends telomeres by adding telomere terminal DNA repetitive sequences to linear homologous chromosomes, thereby delaying cell senescence.

At present, there is a fresh report on the preparation of cosmetics having an anti-aging effect against retrogrowth by adding a telomerase composition. The anti-aging cosmetic prepared by adding the telomerase composition has great significance.

Disclosure of Invention

The invention provides a telomerase composition, which is characterized in that the telomerase composition comprises the following raw materials: 42-46 parts of telomerase, 6-8 parts of telomerase activator, 1-2 parts of carnosine, 1-2 parts of chitin, 1-2 parts of melatonin and 50-60 parts of deionized water.

Preferably, the telomerase is one or more of broccoli telomerase extract, Chinese pine leaf telomerase extract and ginkgo leaf telomerase extract.

Preferably, the telomerase is prepared by mixing a broccoli telomerase extract, a Chinese pine leaf telomerase extract and a ginkgo leaf telomerase extract according to a mass ratio of 1:1: 1.

Preferably, the preparation method of the broccoli telomerase extract, the Chinese pine leaf telomerase extract and the ginkgo leaf telomerase extract comprises the following steps:

a. putting fresh broccoli buds, fresh pine leaves or fresh ginkgo leaves into a mortar, adding liquid nitrogen, grinding into powder, adding the extracting solution, and continuously grinding for 10-20 min;

b. transferring the ground mixture into a centrifuge tube, centrifuging for 15-20 min at 4 ℃ and 13000-16000 rpm/min, and subpackaging the supernatant into an EP tube;

c. adding PEG 6000 into an EP tube, oscillating and mixing for 40-60 min at the constant temperature of 4-7 ℃, centrifuging for 2-4 min at the temperature of 4 ℃ and the rotating speed of 13000-16000 rpm/min, removing supernatant, and taking lower-layer substances to store at the temperature of-80 ℃ to obtain a broccoli telomerase extract or a Chinese pine leaf telomerase extract or a ginkgo leaf telomerase extract.

Preferably, in the step a, the flower buds of the Chinese and western orchids or the pine leaves or the ginkgo leaves: liquid nitrogen: an extract (4-6 g): (4-6 mL): (15 to 25mL)

Preferably, the supernatant in the EP tube in step c (obtained in step b): PEG 6000 ═ 1 mL: (0.18-0.22 g).

Preferably, the extracting solution consists of Tris-HCl buffer solution, magnesium chloride, potassium chloride, ethylene glycol bis (2-aminoethyl ether) tetraacetic acid, phenylmethylsulfonyl fluoride, polyvinylpyrrolidone, glycerol, dithiothreitol and deionized water.

Preferably, the telomerase activator is one or more of rhizoma pinelliae palmatum extract, astragalus extract and astaxanthin.

More preferably, the telomerase active agent is prepared by mixing pinellia ternata extract, astragalus membranaceus extract and astaxanthin according to the mass ratio of 1:1: 1.

The telomerase activator added in the invention can activate the added telomerase, and can also activate the telomerase in cells when entering skin cells, so that the telomerase can give the dying cells increased replication capacity and delay the aging of the cells.

Preferably, the preparation method of the pinellia ternata extract and the astragalus membranaceus extract comprises the following steps:

1) crushing the pinellia ternata or the astragalus membranaceus medicinal material under a wall breaking crusher, sieving, and putting the pinellia ternata or the astragalus membranaceus medicinal material powder into a conical bottle with a stopper;

2) adding methanol and ammonia water into a conical flask with a plug, sealing the plug, performing ultrasonic treatment for 40-60 min in a constant-temperature water bath at 25 ℃, performing suction filtration, and washing for 2 times by using methanol;

3) steaming the filtrate in rotary evaporator to obtain extract, and freeze drying to obtain extract.

Preferably, the rhizoma pinelliae palmatum powder or the astragalus membranaceus powder in the step 2): methanol: ammonia water (3-5 g): (90-110 mL): (10-20 mL).

Preferably, the preparation method of the telomerase composition comprises the following steps: 42-46 parts by weight of telomerase, 6-8 parts by weight of telomerase activator, 1-2 parts by weight of carnosine, 1-2 parts by weight of chitin, 1-2 parts by weight of melatonin and 50-60 parts by weight of deionized water are oscillated and mixed for 15-30 min at the constant temperature of 4-7 ℃.

An anti-aging and anti-retrogressive cosmetic is characterized by comprising the following raw materials:

preferably, the emollient comprises one or more of isodecyl neopentanoate, squalane, and glyceryl monooleate.

Preferably, the emulsifier comprises one or more of sorbitan trioleate, polyethylene glycol fatty acid ester, glyceryl monostearate and polyglycerol-3-methyl glucose distearate.

Preferably, the humectant comprises one or more of glycerol, 1, 3-butanediol, polyethylene glycol and hyaluronic acid.

Preferably, the preservative comprises one or more of imidazolidinyl urea, isothiazolinone, p-hydroxybenzoate and potassium sorbate.

The preparation method of the anti-aging cosmetic for retrograde growth comprises the following steps:

A. adding 8-12 parts by weight of an emollient, 3-6 parts by weight of an emulsifier, 12-16 parts by weight of a humectant, 0.4-0.6 part by weight of a preservative and 28-42 parts by weight of deionized water into a stirring pot, stirring at 70-80 ℃ and 2000-4000 rpm/min for 15-30 min, and cooling to 25-30 ℃ to obtain a mixture;

B. adding 4-8 parts by weight of telomerase composition into the mixture, and stirring at 25-30 ℃ and 300-500 rpm/min for 10-15 min.

The invention has the beneficial effects that:

1. the telomerase composition prepared by adding the broccoli telomerase extract, the Chinese pine leaf telomerase extract, the ginkgo leaf telomerase extract and the telomerase activator has good activity.

2. The anti-aging cosmetic prepared by adding the telomerase composition has excellent anti-aging performance. The anti-aging cosmetic for reverse growth can obviously improve the glossiness of the skin, reduce spots, fade wrinkles and restore the elasticity of the skin after being used for a period of time.

Detailed Description

In the examples, the sources of the raw materials are as follows:

broccoli flower bud: shanghai seven treasures farmer market

PEG 6000: polyethylene glycol 6000, CAS No.: 25322-68-3, the content is more than or equal to 99 percent, and the chemical industry of Jinning Baichuan is limited;

tris (Tris): tris, CAS number: 77-86-1 with the content more than or equal to 99.9 percent, and Hefeibomei Biotech limited;

magnesium chloride: the content is more than or equal to 99 percent, and the chemical industry of Jinning Baichuan is limited;

potassium chloride: the content is more than or equal to 99 percent, and the chemical industry of Jinning Baichuan is limited;

ethylene glycol bis (2-aminoethyl ether) tetraacetic acid: abbreviation EGTA, CAS number: 67-42-5, analytically pure, Shandong Bai Qian chemical Co., Ltd;

phenylmethylsulfonyl fluoride: CAS number: 329-98-6, analytical grade, Shanghai Ciui Biotech Co., Ltd;

polyvinylpyrrolidone: CAS number: 9003-39-8, the content is more than or equal to 99 percent, and Jinning Baichuan chemical industry Co., Ltd;

glycerol: the content is more than or equal to 99.5 percent, and the Guangzhou Fengquan chemical company is limited;

dithiothreitol: CAS number: 3483-12-3% of Bifeibomei Biotech Co., Ltd;

pine needle leaf: purchased from Jinchun nursery in Shaoxing district, Dandong;

ginkgo leaf: from the east garden of Taian Shandong;

pinellia ternata medicinal materials: purchased from the Anhui Bozhou drug market;

astragalus root medicinal materials: purchased from Anhui Bozhou Junfeng pharmaceutical Co Ltd;

carnosine: CAS number: 305-84-0 percent, the content is more than or equal to 99.0 percent, Shandonghui probiotic science and technology limited company;

chitin: CAS number: 9012-76-4 with a content of 99.0% or more, Shandong West Pond Biotech Co., Ltd;

melatonin: CAS number: 73-31-4 percent, the content is more than or equal to 99.5 percent, Sian Rui Ying Biotech limited company;

glycerol monooleate: CAS number: 25496-72-4, the content is more than or equal to 99.5 percent, and bioengineering limited company is created in Anqing;

sorbitan trioleate: CAS number: 26266-58-0%, the content is greater than or equal to 99.0%, the Haian petrochemical plant;

hyaluronic acid: CAS number: 9004-61-9, the content is more than or equal to 99.0%, the molecular weight is about 150-180 ten thousand, and the Henan Xingyi chemical product company Limited;

imidazolidinyl urea: CAS number: 39236-46-9, the content is more than or equal to 99 percent, Wuhan La Na white pharmaceutical chemical industry Co., Ltd;

p-hydroxybenzoic esters: also known as methylparaben: CAS number: 99-76-3, the content is more than or equal to 99 percent, and the product is produced by a Kingning Bai chemical engineering Co.

Example 1

Preparation of broccoli telomerase extract:

putting 5g of fresh broccoli buds into a mortar, adding 5mL of liquid nitrogen, grinding into powder, adding 20mL of extracting solution, and continuously grinding for 10 min;

transferring the ground mixture into a 50mL centrifuge tube, centrifuging for 15min at 4 ℃ and 15000rpm/min, subpackaging the supernatant into 2mL EP tubes, and adding 1mL supernatant into each EP tube;

adding 0.2g PEG 6000 into each EP tube, shaking in a low temperature oscillator at 5 deg.C for 1h, centrifuging at 4 deg.C and 15000rpm/min for 2min, removing supernatant, and collecting lower layer material, and storing at-80 deg.C to obtain stigma croci telomerase extract.

The extracting solution is 1mL of 1mol/L Tris-HCl buffer solution with pH value of 7.5, 4mL of 25mmol/L magnesium chloride, 2mL of 1mol/L potassium chloride, 4mL of 100mmol/L ethylene glycol bis (2-aminoethyl ether) tetraacetic acid, 166 mu L of 12mmol/L benzyl sulfonyl fluoride, 4mL of 7.5 wt% polyvinylpyrrolidone, 4mL of 50 wt% glycerol, 200 mu L of 100mmol/L dithiothreitol and 634 mu L deionized water.

Preparing a telomerase extract of Chinese pine leaves:

putting 5g of fresh pine leaves into a mortar, adding 5mL of liquid nitrogen, grinding into powder, adding 20mL of extracting solution, and continuously grinding for 10 min;

adding 0.2g PEG 6000 into each EP tube, oscillating at 5 deg.C for 1 hr, centrifuging at 4 deg.C and 15000rpm/min for 2min, removing supernatant, and storing the lower layer at-80 deg.C to obtain telomerase extract.

Preparing a ginkgo leaf telomerase extract:

putting 5g of fresh ginkgo leaves into a mortar, adding 5mL of liquid nitrogen, grinding into powder, adding 20mL of extracting solution, and continuously grinding for 10 min;

adding 0.2g PEG 6000 into each EP tube, oscillating at 5 deg.C for 1 hr, centrifuging at 4 deg.C and 15000rpm/min for 2min, removing supernatant, and storing the lower layer at-80 deg.C to obtain folium Ginkgo telomerase extract.

The compositions of the extracting solutions in the preparation of the three telomerase extracts are as follows: 1mL of 1mol/L Tris-HCl buffer, pH 7.5, 4mL of 25mmol/L magnesium chloride, 2mL of 1mol/L potassium chloride, 4mL of 100mmol/L ethylene glycol bis (2-aminoethyl ether) tetraacetic acid, 166. mu.L of 12mmol/L phenylmethylsulfonyl fluoride, 4mL of 7.5 wt% polyvinylpyrrolidone, 4mL of 50 wt% glycerol, 200. mu.L of 100mmol/L dithiothreitol, and 634. mu.L deionized water.

Example 2

Preparation of pinellia ternata extract:

crushing the pinellia ternata medicinal material in a CJ-20 wall breaking crusher of mechanical equipment Limited of Jinan Changji, at the rotation speed of 900rpm/min for 30min, and sieving with a 1000-mesh sieve to obtain pinellia ternata medicinal material powder;

putting 3g of pinellia palmate medicinal powder into a 250mL conical flask with a plug, adding 90mL of methanol and 10mL of ammonia water, and sealing the plug; performing ultrasonic treatment for 60min in a constant-temperature water bath at 25 ℃, performing suction filtration, washing for 2 times by using methanol, using 20mL of methanol for each washing, and collecting the obtained filtrate;

steaming the filtrate in a rotary evaporator at 50 deg.C to obtain extract, and lyophilizing with a lyophilizer to obtain rhizoma Pinelliae Cordatae extract.

Preparing an astragalus extract:

crushing radix astragali in CJ-20 wall-breaking crusher of mechanical equipment Limited in Jinan Changji, at 900rpm/min for 30min, and sieving with 1000 mesh sieve to obtain radix astragali powder;

putting 3g of astragalus medicinal powder into a 250mL conical flask with a plug, adding 90mL of methanol and 10mL of ammonia water, and sealing the plug; performing ultrasonic treatment for 60min in a constant-temperature water bath at 25 ℃, performing suction filtration, washing for 2 times by using methanol, using 20mL of methanol for each washing, and collecting the obtained filtrate;

Example 3

A preparation method of a telomerase composition comprises the following steps:

and (2) oscillating and mixing 45 parts by weight of telomerase, 7 parts by weight of telomerase activator, 2 parts by weight of carnosine, 1 part by weight of chitin, 2 parts by weight of melatonin and 50 parts by weight of deionized water for 20min under a low-temperature oscillator at 5 ℃ to obtain the telomerase composition.

The telomerase is prepared by mixing a broccoli telomerase extract in example 1, a pinus tabulaeformis leaf telomerase extract in example 1 and a ginkgo leaf telomerase extract in example 1 according to a mass ratio of 1:1: 1.

The telomerase activator is prepared by mixing the pinellia ternata extract in example 2, the astragalus extract in example 2 and astaxanthin in a mass ratio of 1:1: 1.

Example 4

Essentially the same as example 3, except that: the telomerase is prepared by mixing a broccoli telomerase extract obtained in example 1 and a pinus tabulaeformis telomerase extract obtained in example 1 according to a mass ratio of 1: 1.

Example 5

Essentially the same as example 3, except that: the telomerase is prepared by mixing the terpineol leaf telomerase extract in example 1 and the ginkgo leaf telomerase extract in example 1 according to the mass ratio of 1: 1.

Example 6

Essentially the same as example 3, except that: the telomerase is prepared by mixing a broccoli telomerase extract obtained in example 1 and a ginkgo leaf telomerase extract obtained in example 1 according to a mass ratio of 1: 1.

Example 7

Essentially the same as example 3, except that: the telomerase activator is prepared by mixing the pinellia ternata extract in example 2 and the astragalus extract in example 2 according to the mass ratio of 1: 1.

Example 8

Essentially the same as example 3, except that: the telomerase activator is prepared by mixing the astragalus extract and the astaxanthin of example 2 according to the mass ratio of 1: 1.

Example 9

Essentially the same as example 3, except that: the telomerase activator is prepared by mixing the pinellia ternata extract and the astaxanthin in the mass ratio of 1:1 in example 2.

Example 10

A method for preparing an anti-aging cosmetic for retrograde growth comprises:

adding 10 parts by weight of emollient, 5 parts by weight of emulsifier, 14 parts by weight of humectant, 0.5 part by weight of preservative and 35 parts by weight of deionized water into a stirring pot, stirring at 70 ℃ and 3000rpm/min for 20min, and cooling to 25 ℃ to obtain a mixture;

adding 6 weight parts of telomerase composition into the mixture, and stirring at 25 deg.C and 400rpm/min for 10 min.

The telomerase composition was prepared as described in example 3.

The emollient is glyceryl monooleate.

The emulsifier is sorbitan trioleate.

The humectant is hyaluronic acid.

The preservative is prepared by mixing imidazolidinyl urea and p-hydroxybenzoate according to the mass ratio of 1: 1.

Example 11

Essentially the same as example 10, except that: the telomerase composition was prepared as described in example 4.

Example 12

Essentially the same as example 10, except that: the telomerase composition was prepared as described in example 5.

Example 13

Essentially the same as example 10, except that: the telomerase composition was prepared as described in example 6.

Example 14

Essentially the same as example 10, except that: the telomerase composition was prepared as described in example 7.

Example 15

Essentially the same as example 10, except that: the telomerase composition was prepared as described in example 8.

Example 16

Essentially the same as example 10, except that: the telomerase composition was prepared as described in example 9.

Test example 1

160 volunteers were randomly divided into 8 groups of 20 individuals each, and anti-aging tests were performed on the cosmetics prepared in examples 10 to 16 with deionized water, respectively. Any cosmetics or external medicines can not be used 3 days before the tested part. Before the experiment, the volunteers needed to clean their faces.

In the experiment, each group of volunteers applied the corresponding sample 2 times every morning and evening, each time according to 1.0mg/cm²The dosage of the composition is uniformly applied, after 8 weeks of continuous use, the applied part of the subject is washed, and skin color and speckle analysis is performed by using a American VISIA facial image analyzer.

TABLE 1 skin gloss enhancement and reduction of total speckle area

	Rate of skin gloss development	Reduction ratio of total spot area
			Deionized water	-0.60％	-0.28％
Example 10	26.90％	30.54％
			Example 11	19.45％	23.46％
Example 12	18.52％	24.32％
			Example 13	18.86％	23.79％
Example 14	17.50％	20.98％
			Example 15	18.62％	21.36％
Example 16	18.39％	21.12％

By comparing deionized water with the cosmetics prepared in examples 10 to 16, an anti-aging test shows that the cosmetics added with the telomerase composition have a remarkable anti-aging and anti-growth effect on skin. Wherein, the skin glossiness is improved by 17.50-26.9%, and the total spot area is reduced by 20.98-30.54%. The reason for this is that: telomerase maintains the continuous proliferation of cells by maintaining telomere length to prevent premature cell senescence.

Test example 2

160 volunteers were randomly selected and divided into 8 groups of 20 persons each, which showed mainly facial skin aging with wrinkles. The cosmetics prepared in examples 10 to 16 were subjected to an anti-aging test using deionized water, respectively. Any cosmetics or external medicines can not be used 3 days before the tested part. Before the experiment, the volunteers needed to clean their faces.

In the experiment, each group of volunteers applied the corresponding sample 2 times every morning and evening, each time according to 1.0mg/cm²The dosage of the composition is uniformly applied, after 8 weeks of continuous use, the applied part of the subject is cleaned, and the change rule of skin texture is analyzed by using a American VISIA facial image analyzer.

TABLE 2 skin texture Change laws

	Rate of change of skin texture
		Deionized water	1.68％
Example 10	-40.26％
		Example 11	-27.68％
Example 12	-26.32％
		Example 13	-28.17％
Example 14	-24.65％
		Example 15	-26.62％
Example 16	-24.38％

Test example 3

160 volunteers were selected and randomly divided into 8 groups of 20 individuals each. The cosmetics prepared in examples 10 to 16 were subjected to an anti-aging test using deionized water, respectively. Any cosmetics or external medicines can not be used 3 days before the tested part. Before the experiment, the volunteers need to measure in the arms.

In the experiment, each group of volunteers smeared with corresponding samples 2 times in the morning and evening at 10 cm distance from the palm base in the left and right arms every day, and the amount of each sample is 1.0mg/cm²The amount of the composition is applied uniformly, and after 8 weeks of continuous use, the applied part of the subject is washed and analyzed for skin elasticity using a skin elasticity tester (Cutometer dual MPA 580).

TABLE 3 rate of change of skin elasticity

	Rate of change of skin elasticity
		Deionized water	2.68％
Example 10	43.54％
		Example 11	32.69％
Example 12	31.64％
		Example 13	29.53％
Example 14	30.81％
		Example 15	31.28％
Example 16	30.17％

The foregoing detailed description of the preferred embodiments of the invention has been presented. It should be understood that numerous modifications and variations could be devised by those skilled in the art in light of the present teachings without departing from the inventive concepts. Therefore, the technical solutions available to those skilled in the art through logic analysis, reasoning and limited experiments based on the prior art according to the concept of the present invention should be within the scope of protection defined by the claims.

Claims

1. A telomerase composition, wherein the telomerase composition comprises: 42-46 parts of telomerase, 6-8 parts of telomerase activator, 1-2 parts of carnosine, 1-2 parts of chitin, 1-2 parts of melatonin and 50-60 parts of deionized water by weight; the telomerase is prepared by mixing a broccoli telomerase extract, a Chinese pine leaf telomerase extract and a ginkgo leaf telomerase extract according to the mass ratio of 1:1: 1; the telomerase activator is prepared by mixing a pinellia ternate extract, an astragalus extract and astaxanthin according to the mass ratio of 1:1: 1.

2. A telomerase composition as claimed in claim 1, wherein: the preparation method of the broccoli telomerase extract, the Chinese pine leaf telomerase extract and the ginkgo leaf telomerase extract comprises the following steps:

a. putting broccoli buds, Chinese pine leaves or ginkgo leaves into a mortar, adding liquid nitrogen, grinding into powder, adding the extracting solution, and continuously grinding for 10-20 min;

b. grinding, transferring into a centrifuge tube, centrifuging for 15-20 min at 4-7 ℃ and 13000-16000 rpm/min, and subpackaging the supernatant into an EP tube;

c. adding PEG 6000 into an EP tube, oscillating and mixing for 40-60 min at the constant temperature of 4-7 ℃, centrifuging for 2-4 min at the temperature of 4-7 ℃ and at the speed of 13000-16000 rpm/min, removing supernatant, and taking lower-layer substances to store at-80 ℃ to obtain a broccoli telomerase extract or a Chinese pine leaf telomerase extract or a ginkgo leaf telomerase extract.

3. A telomerase composition as claimed in claim 2, wherein: the extracting solution consists of Tris-HCl buffer solution, magnesium chloride, potassium chloride, ethylene glycol bis (2-aminoethyl ether) tetraacetic acid, phenylmethylsulfonyl fluoride, polyvinylpyrrolidone, glycerol, dithiothreitol and deionized water.

4. A telomerase composition as claimed in claim 1, wherein: the preparation method of the pinellia ternata extract and the astragalus membranaceus extract comprises the following steps:

3) steaming the filtrate in a rotary evaporator to obtain extract, and freeze drying to obtain rhizoma Pinelliae Cordatae extract or radix astragali extract.

5. A method of preparing a telomerase composition as claimed in any of claims 1 to 4, which comprises the steps of: 42-46 parts by weight of telomerase, 6-8 parts by weight of telomerase activator, 1-2 parts by weight of carnosine, 1-2 parts by weight of chitin, 1-2 parts by weight of melatonin and 50-60 parts by weight of deionized water are oscillated and mixed for 15-30 min at the constant temperature of 4-7 ℃.

6. An anti-aging cosmetic for retrograde growth, which is characterized in that: the anti-aging anti-retrogression cosmetic comprises the following raw materials:

4-8 parts of telomerase composition;

8-12 parts of an emollient;

3-6 parts of an emulsifier;

12-16 parts of a humectant;

0.4-0.6 parts by weight of a preservative;

28-42 parts of deionized water;

wherein the telomerase composition is as claimed in any one of claims 1 to 4.

7. The method for preparing the anti-aging cosmetic for retrograde growth according to claim 6, comprising the steps of: