CN107828650B

CN107828650B - Cell immunotherapy workstation

Info

Publication number: CN107828650B
Application number: CN201710862060.8A
Authority: CN
Inventors: 叶大林; 夏信群
Original assignee: Zhejiang Tailin Bioengineering Co ltd
Current assignee: Zhejiang Tailin Bioengineering Co ltd
Priority date: 2017-09-21
Filing date: 2017-09-21
Publication date: 2020-11-24
Anticipated expiration: 2037-09-21
Also published as: CN107828650A

Abstract

The invention relates to a cellular immunotherapy workstation comprising: the culture transfer cabin is provided with a cabin door and a transfer mechanism for transferring the carbon dioxide incubator on the carbon dioxide incubator transfer system into the cabin; the main operation cabin is internally provided with a centrifuge for blood centrifugal separation and an inactivation heater for serum inactivation; the observation cabin is internally integrated with a cell observation microscope and is used for observing the cell shape, the cell quantity and the cell pollution condition in the cell culture process; wherein, the three cabin bodies are sequentially connected in a sealing way to form a closed working space so as to form a closed immune cell processing and culturing environment; an openable channel is arranged between the adjacent cabin bodies; the front parts of the main operation cabin and the observation cabin are provided with glove positions of a plurality of gloves, so that an operator can manually operate the inside of the cabin outside the cabin. By adopting the technical scheme, the operations of immune cell treatment, culture and the like can be completed in a closed A-level environment.

Description

Cell immunotherapy workstation

Technical Field

The invention relates to biological experiment equipment, in particular to a cellular immunotherapy workstation.

Background

The biological immune cell therapy is a treatment method for achieving the purpose of treating tumors by using biotechnology, and is a fourth major tumor treatment technology after surgery, radiotherapy and chemotherapy, such as methods shown in the publications, namely, a method for preparing a high-efficiency immune active cell group and resisting tumors (publication No. CN 1869206A), a T cell culture method for treating tumors (publication No. CN 107090433A), a tumor specific killer cell preparation and a preparation method thereof (application No. 201410092414.1), and the like. The most critical of the cellular immunotherapy is the cell culture which needs a long period, and the cells need to be continuously replaced and supplemented with culture solution and various factors in the culture process, which all need aseptic operation. In the research process of immune cell therapy and the like, the occurrence of pollution is one of important factors of experimental failure. Once contamination has occurred, it means that the test needs to be restarted, wasting much time and economic cost. With the development of bioscience, the requirements on the sterility of a cell culture environment and the safety of personnel are stricter and stricter, which also puts higher requirements on biological equipment instruments, and the limitations of the traditional GMP laboratory are more and more difficult to meet the requirements of new technology development.

The cell immunotherapy workstation is mainly used for multiple or key process operations of cell separation and extraction, induction and activation, culture and liquid exchange, passage, collection and the like, provides a closed A-level environment for experiments, can integrate multiple functional devices and meets the operation requirements.

Disclosure of Invention

The invention mainly aims to provide a cellular immunotherapy workstation which can provide a closed A-level environment for the operation processes of immune cell treatment, culture and the like.

The technical problem of the invention is mainly solved by the following technical scheme: a cellular immunotherapy workstation, comprising:

the culture transfer cabin is provided with a cabin door and a transfer mechanism for transferring the carbon dioxide incubator on the carbon dioxide incubator transfer system into the cabin;

the main operation cabin is internally provided with a centrifuge for blood centrifugal separation and an inactivation heater for serum inactivation;

the observation cabin is internally integrated with a cell observation microscope and is used for observing the cell shape, the cell quantity and the cell pollution condition in the cell culture process;

wherein, the three cabin bodies are sequentially connected in a sealing way to form a closed working space so as to form a closed immune cell processing and culturing environment; an openable channel is arranged between the adjacent cabin bodies; the front parts of the main operation cabin and the observation cabin are provided with glove positions of a plurality of gloves, so that an operator can manually operate the inside of the cabin outside the cabin.

Through the technical scheme, the culture transfer chamber, the main operation chamber and the observation chamber form a closed working space, and the centrifuge for blood centrifugal separation and the inactivation heater for serum inactivation are arranged in the main operation chamber, so that the operations of immune cell treatment, culture and the like can be completed in a closed A-level environment.

Drawings

FIG. 1 is a schematic diagram of the present invention.

Fig. 2 is a top view of fig. 1.

FIG. 3 is a schematic diagram of a centrifuge.

Fig. 4 is a top view of fig. 3.

Fig. 5 is an enlarged view of fig. 3 at a.

FIG. 6 is an isometric view of a centrifuge.

FIG. 7 is a schematic structural view of an opening of a centrifuge liner.

FIG. 8 is a schematic diagram of a carbon dioxide incubator transfer system.

Fig. 9 is a schematic view of a guide block construction.

Wherein: 1. a centrifuge cover; 2. butting mounting surfaces; 3. framework oil seal; 4. an O-shaped sealing ring; 5. a spring adjustment stud; 6. a variable frequency motor; 7. a rotational speed sensor; 8. overhauling the cover plate; 9. a damping spring; 10. a motor mounting seat; 11. a liquid baffle; 12. an inner container of the centrifugal machine; 13. a horizontal rotor; 14. a centrifuge tube adapter; 15. inflating the sealing strip; 16. a cover lock; 17. a cover handle; 18. a touch screen; 19. a toughened glass window; 20. a machine cover hinge; 21. a disc; 22. positioning a plate; 23. sealing sleeves; 24. an annular pressure plate; 25. a top rod; 26. briquetting; 27. a mechanical bolt; 28. a sensor; 29. a hook part; 30. a U-shaped frame; 31. an outer frame; 32. a transverse frame plate; 33. a vertical frame plate; 34. a platen; 35. a vertical plate; 101. pushing a cart; 102. an upper layer slide rail; 103. an upper layer trolley; 104. a carbon dioxide gas storage tank; 105. a UPS power supply; 106. a carbon dioxide incubator; 107. a timer; 108. a buzzer; 109. a lower layer slide rail; 110. a guide block; 1001. a carbon dioxide incubator transfer system; 1002. a culture transport compartment; 1003. a main operating cabin; 1004. an observation cabin; 1005. a main transfer pod; 1006. a rapid transfer hatch; 1007. a centrifuge; 1008. deactivating the heater; 1009. a waste channel; 1010. a glove position.

Detailed Description

The technical scheme of the invention is further specifically described by the following embodiments and the accompanying drawings.

Example (b): the invention relates to a cellular immunotherapy workstation, as shown in the attached figures 1 and 2, which comprises: a culture transport compartment 1002, a main operating compartment 1003 and an observation compartment 1004.

A culture transfer chamber 1002 having a chamber door and a transfer mechanism (e.g., a slide rail, a robot, or a robot) for transferring the carbon dioxide incubator 106 on the carbon dioxide incubator transfer system 1001 into the chamber; after the carbon dioxide incubator transfer trolley is in butt joint with the transfer cabin, the carbon dioxide incubator is automatically moved to a set position by the automatic push-pull device or the mechanical arm. When the carbon dioxide incubator needs to be transferred from the cabin to the outside of the cabin, the incubator is automatically pushed to the incubator transfer trolley by the automatic push-pull device or the mechanical arm. The culture transfer cabin is mainly used for safely transferring cells to be proliferated and cultured inside and outside the workstation, and for example, the cells to be proliferated and cultured can be cleaned and sterilized inside the workstation for treating B individual cells when the A individual cells are transferred outside the workstation for continuous culture after treatment; compared with the method that the incubator is fixed in the workstation, the working efficiency is greatly improved.

A main operation cabin 1003, in which a centrifuge 1007 for centrifugal separation of blood and an inactivation heater 1008 for inactivation of serum are arranged, and which is provided with a waste channel 1009; the main operation cabin completes most of operation processes of cell culture, including centrifugal separation of cells, liquid feeding culture, plasma inactivation and rapid factor transfer.

And an observation cabin 1004, in which a cell observation microscope is integrated, for observing cell morphology, quantity and contamination during cell culture.

The three cabin bodies of the culture transfer cabin 1002, the main operation cabin 1003 and the observation cabin 1004 are sequentially and hermetically connected to form a closed working space so as to form a closed immune cell treatment and culture environment; an openable and closable passage (such as a hatch door which is opened to form the passage and closed to separate the cabins) is arranged between the adjacent cabins; a plurality of glove spaces 1010 for manual operation of the inside of the main operation cabin 1003 and the observation cabin 1004 are arranged at the front of the cabin.

The main transfer module 1005 is disposed on one side of the observation module 1004 for transferring the subsequent large items required in the main operation module.

The main operating cabin 1003 is provided with a plurality of fast transfer hatches 1006, the inner sides of the fast transfer hatches 1006 facing the operating platform of the main operating cabin 1003. The rapid transfer cabin door can finish sterilization in a very short speed, and rapidly transfer tools, factors and other articles which are urgently needed or articles required by experiments to the main operation platform.

As shown in fig. 3, 4, 5, 6 and 7, the centrifuge comprises a centrifuge inner container 12, a variable frequency motor 6 and a motor mounting base 10; a centrifuge inner container 12, the bottom end of which is closed, the upper end of which is opened, and the opening part of which covers the centrifuge cover 1 and is connected in a sealing way; a built-in horizontal rotor 13 and a centrifuge tube adapter 14; the variable frequency motor 6 is vertically arranged at the lower part of the inner container 12 of the centrifuge, the shaft end of a motor shaft penetrates through the bottom wall of the inner container 12 of the centrifuge to enter a centrifugal chamber (namely the inner container 12 of the centrifuge) to be connected with the horizontal rotor 13, and the penetrating part is provided with sealing connection; the motor mount pad 10, the main part is the barrel, mid-mounting inverter motor 6, top sealing connection in centrifuge inner bag 12 bottom, bottom sealing connection has maintenance apron 8. And O-shaped sealing rings 4 are arranged on the connecting parts of the motor mounting seat 10 and the centrifuge inner container 12 and the connecting parts of the overhaul cover plate 8 and the motor mounting seat 10.

In the structure, the centrifuge cover 1, the centrifuge inner container 12, the motor mounting seat 10 and the overhaul cover plate 8 are enclosed into a closed space, and in the closed space, the space where the centrifuge inner container 12 is located and the space where the motor mounting seat 10 is located are in an isolated state; the isolation structure is as follows: the upper part of the variable frequency motor 6 is provided with a boss, and a sealing sleeve 23 is arranged on the boss; the main body of the sealing sleeve 23 is a semi-closed cylinder structure with an opening at the bottom, the closed end is sleeved on the motor shaft and provided with a framework oil seal 3 at the joint part to form sealing connection, the open end extends outwards and radially to form an annular table, the annular table is provided with a liquid baffle plate 11 with an annular plate-shaped elastic structure and a pressing block 26 for pressing the inner edge of the liquid baffle plate 11 on the annular table to form sealing connection, and the outer edge of the liquid baffle plate 11 is attached to the bottom wall of the centrifuge liner 12 of the penetrating part and is pressed through an annular pressing plate 24 to form sealing connection; the liquid barrier 11 is an elastic body that deforms under pressure to form a seal at the pressure contact surface. Firstly, in the structure, the variable frequency motor 6 directly enters the centrifugal part to provide centrifugal force, other power transmission structures are not needed, the power transmission path is shortest, the transmission efficiency is improved, and the overall volume is effectively reduced while power is provided for the centrifugal part; secondly, the structure is reliable in separation, and under the vibration of the operation of the variable frequency motor 6, the two spaces can still be ensured to be always in a separation state, so that a good separation effect is provided; in addition, because the liquid baffle plate 11 is an elastic body, the structure can conveniently adjust the transverse position of the variable frequency motor 6.

One side of the centrifuge cover 1 is rotationally connected with the centrifuge inner container 12 through a cover hinge 20, and a cover handle 17 is arranged at the side edge of the centrifuge cover 1 opposite to the cover hinge 20; the cover handle 17 has a holding portion, the front end of which extends into a clamping portion; the top end wall of the opening part of the centrifuge inner container 12 is provided with a mechanical lock, the mechanical lock comprises a lock body and a mechanical bolt 27 which can rotate on the lock body, and the outside of the bottom of the lock body extends laterally to form a hook part 29; when the turning centrifugal cover 1 is buckled, the clamping part of the cover handle 17 is pressed on the hook part 29, then the mechanical lock tongue 27 is rotated, and the mechanical lock tongue 27 is pressed on the clamping part to form locking action. The clamping part is extended from the holding part of the cover handle 17, the mechanical lock is matched with the clamping part, the opening and closing state of the centrifuge cover 1 is converted, the clamping part is not in place and cannot be locked, the hook part 29 is not separated from the clamping part and cannot open the centrifuge cover 1, and the safety of the cover closing action of the centrifuge cover 1 is greatly improved. The mechanical lock matched with the clamping part has the advantages of simple structure, low cost, easy manufacture, convenient use and high reliability.

In order to improve the convenience and safety of operation and the working efficiency of the whole machine, the clamping part of the cover handle 17 is columnar, the upper side and the lower side of the cover handle are both provided with grooves, and a sensor 28 (such as a Hall sensor) is respectively arranged in each groove; an inflatable sealing strip 15 is arranged at the opening part of the centrifuge inner container 12; when the cover is closed, the sensor 28 at the lower side of the clamping part sends a signal, and the system inflates and expands the inflatable sealing strip 15 so as to form sealing connection between the inner peripheral wall of the inner container 12 of the centrifuge and the outer side wall of the centrifuge cover 1; then the mechanical lock tongue 27 is rotated to press the clamping part to form a safety mechanism for starting or not starting the variable frequency motor 6; when the sensor on the upper side of the card part is locked and kept locked, the system starts the centrifugal action, and once the locked state is lost due to abnormal conditions, the centrifugal action is automatically stopped, thereby improving the safety. The clamping part adopting the structure has high strength, higher bearing capacity, long service life, wear resistance and difficult damage. Through the sensor at the lower side of the clamping part, the inflation work of the inflatable sealing strip can be automatically started after the cover closing action is finished, so that the centrifuge cover 1 is hermetically connected to the centrifuge inner container; the structure and the mode not only facilitate the operation, but also improve the safety, simplify the operation and improve the production efficiency.

The invention provides a better sealing structure for the sealing scheme of the opening part of the inner container 12 of the centrifugal machine: an outer frame 31 is arranged on the periphery of the opening part of the centrifuge inner container 12; the outer frame 31 is annular, the cross section is L-shaped, including horizontal frame plate 32 and vertical frame plate 33, the horizontal frame plate 32 is welded on the peripheral surface of the centrifuge inner container 12; a bedplate 34 is horizontally arranged at the top end of the vertical frame plate 33, and the bedplate 34 is a hollow annular flat plate; the top end of the opening part of the centrifuge inner container 12 is transversely provided with a U-shaped frame 30, the notch of a U-shaped groove of the U-shaped frame 30 points to the middle part of the centrifuge inner container 12, and an inflatable sealing strip 15 is arranged in the groove; the outer side part of the U-shaped frame 30 is connected with the inner side wall of the vertical frame plate 33, and the upper side part is connected with the bottom surface of the bedplate 34; the centrifuge cover 1 is of a double-layer structure, and vertical plates 35 are vertically arranged on the outer peripheries of structures on two sides; when the cover is closed, the vertical plate 35 is opposite to the notch of the U-shaped frame 30, and the inflatable sealing strip 15 abuts against the vertical plate 35 to form sealing connection when inflated. The main bodies of the outer frame 31, the U-shaped frame 30 and the bedplate 34 are all annular bodies, wherein the outer frame 31 is of an L-shaped structure and has high structural strength; the lower side of the U-shaped frame 30 is transversely arranged at the top end of the side wall of the opening part of the inner container 12 of the centrifugal machine, the bottom of the U-shaped frame is attached to the outer frame 31, and the upper side of the U-shaped frame is attached to the bedplate 34, so that a stable sealing and supporting structure is provided for the inflatable sealing strip 15, the supporting strength is high, the deformation is not easy, the manufacture is easy; the centrifuge inner container 12 forms good sealing fit with the vertical plate 35 of the centrifuge cover 1 through the sealing structure; meanwhile, the upper outer edge (the part outside the vertical plate 35) of the upper layer structure of the centrifuge cover 1 is arranged on the bedplate 34 or the clamping part of the cover handle forms a gravity supporting structure of the centrifuge cover 1, and the hook part 29 forms a limiting structure through the clamping part of the cover handle, so that the inflatable sealing strip 15 does not need to bear vertical acting force and only bears transverse acting force as sealing, and the sealing effect is better.

As a main body of the driving part, the variable frequency motor 6 is easy to generate vibration during operation, especially during shifting and speed changing, and influences the test; therefore, the invention provides a better fixing scheme of the variable frequency motor 6: the upper part of the variable frequency motor 6 is provided with a disc 21, and the bottom of the disc 21 is sleeved with a positioning disc 22; a plurality of annularly arranged convex pins are arranged on the positioning disc 22 and the motor mounting base 10 in a one-to-one correspondence manner; a damping spring 9 is sleeved on the convex pins corresponding to the upper part and the lower part; the positioning disc 22 is provided with a top rod 25, and the top rod 25 is abutted against an annular pressing plate 24 on the bottom wall of the centrifuge inner container 12 of the penetrating part. The fixing structure has extremely compact structure, easy installation, reliable fixation and better shock absorption effect; particularly, the whole machine is effectively maintained in a small volume range while a good damping effect is provided.

The mounting part of the convex pin on the motor mounting seat 10 is of a stepped structure, the small end of the stepped end forms a spring adjusting stud 5, and the spring adjusting stud 5 penetrates through the bottom plate of the motor mounting seat 10 and is fixed through a nut so as to finely adjust the transverse position of the variable frequency motor 6. According to the structural scheme, the variable frequency motor 6 can be transversely adjusted by utilizing the gap between the damping spring and the convex pin (the diameter of the convex pin is smaller than the inner diameter of the damping spring) and the gap between the small end of the stepped end of the convex pin and the mounting hole (the diameter of the small end of the stepped end of the convex pin is smaller than the diameter of the mounting hole), so that the aim of transversely adjusting the position of the horizontal rotor in the inner container of the centrifuge is fulfilled; from another point of view, by means of the technical solution of the present invention, the lateral position of the horizontal rotor in the centrifugal chamber can be adjusted by adjusting the lateral position of the variable frequency motor 6, which is not available in the prior art, and is one of the inventive achievements of the present invention.

The convex pin on the positioning disc 22 is screwed and fixed, the thread end extends to form an ejector rod 25, and the head of the ejector rod 25 is spherical or is embedded with a rotatable spherical body. When installing and adjusting inverter motor 6, be convenient for remove, avoid damaging the part with the ejector pin contact, be convenient for especially finely tune inverter motor 6's horizontal position.

The bottom of the motor mounting seat 10 is provided with a mounting hole and a plurality of convex pins, and the bottom end of the variable frequency motor 6 penetrates out of the mounting hole; the bottom end of the variable frequency motor 6 penetrates out of the mounting hole, and a rotating speed sensor 7 is arranged in the middle of the penetrating end.

As shown in fig. 8 and fig. 9, the carbon dioxide incubator transfer system comprises: an upper trolley 103, a carbon dioxide storage tank 104, a UPS 105 and a trolley 101.

The upper layer trolley 103 is used for placing a carbon dioxide incubator 106 during conveying, and comprises a box body, and rollers are arranged at the bottom of the box body.

And a carbon dioxide gas tank 104 provided in the upper carriage 103 and supplying gas to a carbon dioxide incubator 106 provided in the upper carriage during transportation so that the carbon dioxide incubator 106 maintains a desired internal gas atmosphere.

And the UPS 105 is arranged on the box body of the upper layer trolley 103, supplies power to the carbon dioxide incubator on the upper layer trolley during conveying, and supplies electric heating to the carbon dioxide incubator to maintain the culture temperature.

The trolley 101 is provided with a platform at the upper part, an upper sliding rail 102 for guiding the rollers at the bottom of the box body of the upper trolley to slide into the placement position of the upper trolley is arranged on the platform, the upper sliding rail 102 is of a groove body structure matched with the rollers, and the direction is that the front end of the trolley points to the rear end; and the upper trolley and the carbon dioxide incubator are moved out or pulled into the platform by using the upper slide rail. The front part of the cart is provided with a guide block 110, so that the guide block is hung with docking equipment (namely a main operation cabin); when the cart approaches the docking device, the guide blocks are matched with corresponding structures (such as holes, grooves and the like) on the docking device, so that the cart enters an accurate position to ensure that the upper-layer slide rail is aligned with the end part of the slide rail on the docking device, and the upper-layer cart and the carbon dioxide incubator can be quickly and accurately transferred between the cart and the docking device.

In order to further improve the accuracy of the docking action, the following further optimization scheme can be adopted, as shown in fig. 9, the guide blocks 110 are two pairs and are respectively arranged at two ends of the front part of the cart; the guide block is provided with an open slot, the bottom of the open slot is arc-shaped, and the slot is an outward-expanded flaring; wherein, the open slot of one guide block is positioned at the front end part of the guide block, and the open slot of the other guide block is positioned at the inner side part; two guide columns are arranged outside the butt joint side of the butt joint equipment, each guide column is provided with a cylindrical section, and the diameter of each cylindrical section is matched with the arc-shaped bottom of each open slot; when in butt joint, the cart is close to the butt joint equipment, the guide block with the open slot at the front end is clamped into the corresponding guide column, the circular arc bottom is abutted against the cylindrical section of the guide column, then the cart is pushed to rotate by taking the guide column as the center until the open slot at the side part of the other guide block is clamped into the cylindrical section of the other guide column, so that the positioning of the cart and the alignment of the upper-layer slide rail are completed. The structure enables the cart to more accurately enter the butt joint position and be aligned in two vertical directions, the accuracy of alignment of the upper-layer slide rail on the cart and the end part of the slide rail on the butt joint equipment is improved, the transfer of the upper-layer cart and the carbon dioxide incubator is faster and more stable, and the situation that the alignment accuracy is insufficient to cause the carbon dioxide incubator to vibrate in the transfer process to influence the culture state of a culture in the carbon dioxide incubator is avoided.

In actual use, due to different specifications of the docking equipment, the docking positions of the cart are different; therefore, the invention provides a better technical scheme: the platform at the upper part of the cart is a movable platform, and the moving direction is approximately the same as the guiding direction of the upper-layer guide rail; the mechanism for driving the platform to move comprises a lower slide rail 109 arranged at the bottom of the platform and a screw driving mechanism. The platform can be fixed on the cart in a sliding way through the lower sliding rail; operating the screw driving mechanism to drive the platform to move; after the cart is aligned with the docking equipment, the position of the platform can be adjusted in real time through the spiral driving mechanism, so that the upper layer cart and the carbon dioxide incubator enter better transfer positions.

For the culture of cells, tissues and some special microorganisms, the external environment purification degree is improved, the pollution from the outside is reduced, and the success rate of the culture is favorably improved; the interior of the box body of the upper layer trolley is not easy to degerming, and the regular degerming is needed; even if the sterilization is carried out regularly, the external pollution is not easy to completely eradicate. Therefore, the invention provides a better solution, and the box body of the upper layer trolley is set to be a closed structure with an internal space isolated from an external space; after the sealed structure is adopted, the inside and the outside of the box body are completely isolated, regular sterilization is not needed, pollution sources are completely isolated, and labor intensity is reduced.

In order to further reduce the source of bacterial pollution, the carbon dioxide gas storage tank 104 is arranged inside the upper layer trolley box body, the gas pipe of the carbon dioxide gas storage tank penetrates through the side wall of the box body, and the penetrating part is provided with a sealing ring to form sealing connection. The UPS power supply is arranged in the upper layer trolley box body, a cable of the UPS power supply penetrates through the side wall of the box body, and the penetrating part is provided with a sealing ring to form sealing connection. The carbon dioxide gas storage tank and the UPS are arranged in the closed box body of the upper trolley and are completely isolated from the external space. The sealed box body is of a fabricated structure, for example, a structure having a cover, and after the carbon dioxide gas storage tank and the UPS power supply are installed in the box body, the cover is installed, and a joint portion between the cover and the box body is provided with a sealing connection.

Because the UPS power supply can generate certain heat when working, although the heat is smaller, in order to ensure the stability of long-term operation, the invention provides a better alternative scheme: the upper part of the box body of the upper layer trolley is provided with a heat insulation structure, so that heat is prevented from being directly transferred to the carbon dioxide incubator; the side part or the bottom part of the box body is provided with a heat conducting part made of heat conducting materials so as to transfer heat in the box body to the outside of the box body through heat transfer; the UPS power supply is provided with a metal component for heat conduction at the outer side, and the tail end of the metal component is connected with a heat conduction part on the box body of the upper layer trolley. The heat of the UPS is transferred to the heat conducting part through the metal component, and the heat conducting part is transferred to the outer side of the box body, so that the temperature of the UPS during working is reduced.

The carbon dioxide incubator transfer system improves the existing problems based on the basic requirements of users on incubators, provides the technical scheme of a transfer vehicle consisting of an upper trolley and a trolley, and is provided with a UPS (uninterrupted power supply) and a carbon dioxide gas storage tank in the upper trolley, so that the carbon dioxide incubators can continuously supply power and gas in the transfer process. The upper layer trolley has a timing function, when the UPS is cut off and charging starts to time, timing time can be set, the timer 107 times, and the buzzer 108 gives an alarm to prompt charging. In the actual use process, especially for the culture process of a longer period of immune cell culture, the situations of transferring, power failure and gas (carbon dioxide) cutoff of a carbon dioxide incubator are effectively avoided.

With the development of bioscience, the requirements on the sterility of a cell culture environment and the safety of personnel are stricter and stricter, which also puts higher requirements on biological equipment instruments, and the limitations of the traditional GMP laboratory are more and more difficult to meet the requirements of new technology development. Bioscience has created an urgent need for convenient, safe, efficient, and intelligent biological devices. Therefore, the inventor provides a technical scheme of a cell immunotherapy workstation, which is mainly used for a plurality of or key process operations of cell separation and extraction, induction and activation, culture and liquid change, passage, collection and the like, provides a closed A-level environment for experiments, can integrate a plurality of functional devices and meets the experiment requirements; the requirements of biological science on convenient, safe, efficient and intelligent biological equipment are met. In the cellular immunotherapy workstation, the space of a closed A-level environment is limited, the existing centrifugal machine is large in size, a bacterial growth environment is easily formed in the centrifugal machine, and the working space in the centrifugal machine is easily communicated with an external test environment to form potential infection hidden dangers.

The embodiments in the description are only for illustrating the present invention and do not limit the scope of the present invention. The scope of the present invention is defined only by the appended claims, and any omissions, substitutions, or modifications made based on the embodiments disclosed herein will fall within the scope of the present invention.

Claims

1. A cellular immunotherapy workstation, characterized by comprising:

a culture transfer cabin (1002) having a cabin door and a transfer mechanism for transferring the carbon dioxide incubator (106) on the carbon dioxide incubator transfer system (1001) into the cabin;

a main operation cabin (1003) which is internally provided with a centrifuge (1007) for blood centrifugal separation and an inactivation heater (1008) for serum inactivation;

and an observation cabin (1004) which is integrated with a cell observation microscope and is used for observing the cell shape, the cell quantity and the cell pollution condition in the cell culture process;

wherein, the three cabin bodies are sequentially connected in a sealing way to form a closed working space so as to form a closed immune cell processing and culturing environment; an openable channel is arranged between the adjacent cabin bodies; a plurality of glove positions (1010) for an operator to manually operate the interior of the main operation cabin (1003) and the observation cabin (1004) are arranged at the front parts of the main operation cabin and the observation cabin;

the centrifuge includes:

the bottom end of the centrifuge inner container (12) is closed, the upper end of the centrifuge inner container is opened, and the opening part seals and covers the centrifuge cover (1) and is connected in a sealing way; a built-in horizontal rotor (13) and a centrifuge tube adapter (14);

the variable frequency motor (6) is vertically arranged at the lower part of the centrifuge inner container (12), the shaft end of a motor shaft penetrates through the bottom wall of the centrifuge inner container (12) to enter a centrifugal chamber to be connected with the horizontal rotor (13), and the penetrating part is hermetically connected;

the motor mounting seat (10) is a cylinder body, the middle part of the motor mounting seat is provided with the variable frequency motor (6), the top end of the motor mounting seat is hermetically connected to the bottom of the centrifuge inner container (12), and the bottom end of the motor mounting seat is hermetically connected with the maintenance cover plate (8);

the upper part of the variable frequency motor (6) is provided with a boss, and a sealing sleeve (23) is arranged on the boss; the main body of the sealing sleeve (23) is of a semi-closed cylinder structure with an opening at the bottom, the closed end is sleeved on the motor shaft and provided with a framework oil seal (3) at a joint part to form sealing connection, the open end extends outwards and radially to form an annular table, the annular table is provided with a liquid baffle plate (11) with an annular plate-shaped elastic structure and a pressing block (26) for pressing the inner edge of the liquid baffle plate (11) on the annular table, and the outer edge of the liquid baffle plate (11) is attached to the bottom wall of the centrifuge liner (12) of the penetrating part and is pressed through an annular pressing plate (24).

2. A cellular immunotherapy workstation according to claim 1, characterized in that: the device also comprises a main transfer cabin (1005) which is arranged at one side of the observation cabin (1004) and is used for transferring subsequent large articles required in the main operation cabin.

3. A cellular immunotherapy workstation according to claim 1, characterized in that: the main operation cabin (1003) is provided with a plurality of quick transfer cabin doors (1006), and the inner sides of the quick transfer cabin doors (1006) face to an operation table of the main operation cabin (1003).

4. A cellular immunotherapy workstation according to claim 1, 2 or 3, characterized in that: one side of the centrifuge cover (1) is rotatably connected with the centrifuge inner container (12), and a cover handle (17) is arranged at the side edge of the centrifuge cover (1) at the opposite side; the cover handle (17) is provided with a holding part, and the front end of the holding part extends into a clamping part; the top end wall of the opening part of the centrifuge inner container (12) is provided with a mechanical lock, the mechanical lock comprises a lock body and a mechanical bolt (27) which can rotate on the lock body, and the outer side of the bottom of the lock body extends laterally to form a hook part (29); when the turnover centrifuge cover (1) is buckled, the clamping part of the cover handle (17) is pressed on the hook part (29), then the mechanical lock tongue (27) is rotated, and the mechanical lock tongue (27) is pressed on the clamping part to form locking action.

5. A cellular immunotherapy workstation according to claim 4, characterized in that: the clamping part of the cover handle (17) is columnar, the upper side and the lower side of the cover handle are both provided with grooves, and a sensor (28) is arranged in each of the two grooves; an inflatable sealing strip (15) is arranged at the opening part of the centrifuge inner container (12); when the cover is closed, a sensor (28) at the lower side of the clamping part sends a signal, and the system inflates and expands the inflatable sealing strip (15) so as to form sealing connection between the inner peripheral wall of the inner container (12) of the centrifuge and the outer side wall of the centrifuge cover (1); then the mechanical lock tongue (27) is rotated to press the clamping part to form a safety mechanism for starting or not of the variable frequency motor (6).

6. A cellular immunotherapy workstation according to claim 1, 2 or 3, characterized in that: the carbon dioxide incubator transfer system (1001) comprises:

the upper layer trolley (103) is used for placing a carbon dioxide incubator (106) during conveying and comprises a box body, and rollers are arranged at the bottom of the box body;

a carbon dioxide gas storage tank (104) which is arranged on the box body of the upper layer trolley (103) and supplies gas to a carbon dioxide incubator (106) on the upper layer trolley (103) during transportation so that the carbon dioxide incubator (106) maintains a required internal gas environment;

the UPS power supply (105) is arranged on the box body of the upper trolley (103), supplies power to the carbon dioxide incubator (106) on the upper trolley (103) during conveying, and supplies electric heating to the carbon dioxide incubator (106) to maintain the culture temperature;

and the upper part of the trolley (101) is provided with an upper-layer slide rail (102) for guiding the bottom roller of the upper-layer trolley (103) to slide into the placing position.

7. A cellular immunotherapy workstation according to claim 6, characterized in that: the box body of the upper layer trolley (103) is a closed structure with an internal space isolated from an external space; the UPS (105) is arranged in the box body of the upper layer trolley (103), a cable of the UPS penetrates through the side wall of the box body, and a penetrating part of the UPS is provided with a sealing ring to form sealing connection; the side part or the bottom part of the box body of the upper layer trolley (103) is provided with a heat conducting part made of heat conducting materials so as to transfer heat in the box body to the outside of the box body through heat transfer; a metal component for heat conduction is arranged on the outer side of the UPS (105), and the tail end of the metal component is connected with a heat conduction part on the box body of the upper layer trolley (103).

8. A cellular immunotherapy workstation according to claim 6, characterized in that: the front part of the trolley (101) is provided with a guide block (110) which is hung with docking equipment; the guide blocks (110) are divided into two pairs and are respectively arranged at two ends of the front part of the trolley (101); the guide block (110) is provided with an open slot, the bottom of the open slot is arc-shaped, and the open slot is an outward-expanded flaring; wherein, the open slot of one guide block (110) is positioned at the front end part of the guide block (110), and the open slot of the other guide block (110) is positioned at the inner side part; two guide columns are arranged outside the butt joint side of the butt joint equipment, each guide column is provided with a cylindrical section, and the diameter of each cylindrical section is matched with the arc-shaped bottom of each open slot; when in butt joint, the trolley (101) is close to the butt joint equipment, a guide block with an open slot at the front end is clamped into the corresponding guide column, the arc-shaped bottom is abutted against the cylindrical section of the guide column, then the trolley (101) is pushed to rotate by taking the guide column as the center until the open slot at the side part of the other guide block (110) is clamped into the cylindrical section of the other guide column, so that the positioning of the trolley (101) and the alignment of the upper-layer slide rail (102) are completed.