CN107828650A - A kind of cellular immunotherapy work station - Google Patents
A kind of cellular immunotherapy work station Download PDFInfo
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- CN107828650A CN107828650A CN201710862060.8A CN201710862060A CN107828650A CN 107828650 A CN107828650 A CN 107828650A CN 201710862060 A CN201710862060 A CN 201710862060A CN 107828650 A CN107828650 A CN 107828650A
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M45/00—Means for pre-treatment of biological substances
- C12M45/05—Means for pre-treatment of biological substances by centrifugation
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/34—Internal compartments or partitions
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/52—Mobile; Means for transporting the apparatus
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/12—Means for regulation, monitoring, measurement or control, e.g. flow regulation of temperature
- C12M41/18—Heat exchange systems, e.g. heat jackets or outer envelopes
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M45/00—Means for pre-treatment of biological substances
- C12M45/20—Heating; Cooling
Abstract
The present invention relates to a kind of cellular immunotherapy work station, it includes：Culture transports cabin, has the hatch door and transfer device being transferred to the CO2gas incubator in CO2gas incubator transfer system in cabin；Main operation cabin, the built-in inactivation heater for the centrifuge of centrifugal blood separation and for inactivating blood serum；And astrodome, its interior integrating cell viewing microscope, for observing cellular morphology, quantity and pollution condition in cell cultivation process；Wherein, above three nacelle is tightly connected into closed working space successively, to form the processing of the immunocyte of closing, culture environment；There is passage able to turn on or off between adjacent nacelle；Main operation cabin and the anterior gloves position for installing several gloves of astrodome, for operator out of my cabin to being operated manually in cabin.Pass through above-mentioned technical proposal, the operations such as immunocyte processing, culture are made to be completed in the environment of a closed A level.
Description
Technical field
The present invention relates to a kind of Bioexperiment equipment, more particularly, to a kind of cellular immunotherapy work station.
Background technology
Biological immune cell therapy is a kind for the treatment of method for the purpose for reaching treatment tumour with biotechnology, is after hand
The fourth-largest oncotherapy technology after art, radiation and chemotherapy, such as entitled " a kind of efficiently immunocompetent cell group preparation and use
In antineoplastic method "（Publication number CN1869206A）, the T cell cultural method of oncotherapy " be used for "（Publication No.：
CN107090433A）, " a kind of tumor-specific cytolytic T lymphocytes preparation and preparation method thereof "（Application number 201410092414.1）
Deng the method shown in public technology.Cellular immunotherapy is it is crucial that the culture of cell, the culture needs of cell are one longer
Cycle, in incubation, cell need constantly change, add nutrient solution and the various factors, these are required for sterile behaviour
Make.In research process such as immune cell therapies, one of an important factor for pollution is the failure of an experiment occurs.Once pollute,
It will imply that experiment needs restart, waste many times and financial cost.With the development of bioscience, cell is trained
The aseptic of foster environment and the security requirement to personnel are more and more stricter, and this also proposes higher want to biological plant instrument
Ask, and the limitation in traditional GMP laboratories is increasingly difficult to the requirement for meeting development of new techniques.
Cellular immunotherapy work station, it is mainly used in the separation and Extraction of cell, liquid, passage, collection are changed in induced activation, culture
Operated etc. multinomial or critical process, a closed A level environment is provided for experiment, while multiple function equipment can be integrated, it is full
Foot operation requires.
The content of the invention
Present invention is primarily intended to provide a kind of cellular immunotherapy work station, it can be immunocyte processing, culture
A closed A level environment is provided Deng operating process.
The above-mentioned technical problem of the present invention is mainly what is be addressed by following technical proposals：A kind of cellular immunotherapy
Work station, it includes：
Culture transports cabin, has the cabin being transferred to the CO2gas incubator in CO2gas incubator transfer system in cabin
Door and transfer device；
Main operation cabin, the built-in inactivation heater for the centrifuge of centrifugal blood separation and for inactivating blood serum；
And astrodome, its interior integrating cell viewing microscope, for observing cellular morphology, quantity and dirt in cell cultivation process
Dye situation；
Wherein, above three nacelle is tightly connected into closed working space successively, to form the processing of the immunocyte of closing, training
Support environment；There is passage able to turn on or off between adjacent nacelle；Main operation cabin and the anterior gloves for installing several gloves of astrodome
Position, for operator out of my cabin to being operated manually in cabin.
Pass through above-mentioned technical proposal, the closed work that the present invention is formed using culture transhipment cabin, main operation cabin and astrodome
Make space, and be provided in main operation cabin the centrifuge of centrifugal blood separation and the inactivation heater for inactivating blood serum,
The operations such as immunocyte processing, culture are made to be completed in the environment of a closed A level.
Brief description of the drawings
Accompanying drawing 1 is a kind of structural representation of the present invention.
Accompanying drawing 2 is the top view of accompanying drawing 1.
Accompanying drawing 3 is a kind of structural representation of centrifuge.
Accompanying drawing 4 is the top view of accompanying drawing 3.
Accompanying drawing 5 is enlarged drawing at the A of accompanying drawing 3.
Accompanying drawing 6 is the axonometric drawing of centrifuge.
Accompanying drawing 7 is a kind of structural representation for centrifuging machine liner opening portion.
Accompanying drawing 8 is a kind of structural representation of CO2gas incubator transfer system.
Accompanying drawing 9 is a kind of structural representation of guide pad.
Wherein：1st, centrifuge cup；2nd, abutting joint face；3rd, outside framework oil seal；4th, O-ring seal；5th, spring regulation stud；6、
Variable-frequency motor；7th, speed probe；8th, access panel；9th, damping spring；10th, motor mount；11st, liquid barrier；12nd, centrifuge
Inner bag；13rd, horizontal rotor；14th, centrifuge tube adapter；15th, gas-flow closure bar；16th, hood lock；17th, cover handle；18th, touch
Screen；19th, safety glass form；20th, cover hinge；21st, disk；22nd, positioning disk；23rd, sealing shroud；24th, annular pressing plate；25th, push up
Bar；26th, briquetting；27th, mechanical dead bolt；28th, sensor；29th, hook part；30th, U-shaped frame；31st, outrigger；32nd, transverse frame plate；33rd, vertical frame
Plate；34th, platen；35th, riser；101st, stroller；102nd, upper strata slide rail；103rd, upper trolley；104th, carbon dioxide air accumulator；105、
Ups power；106th, CO2gas incubator；107th, timer；108th, buzzer；109th, lower floor's slide rail；110th, guide pad；
1001st, CO2gas incubator transfer system；1002nd, culture transhipment cabin；1003rd, main operation cabin；1004th, astrodome；1005、
Main transferring cabin；1006th, hatch door is quickly transmitted；1007th, centrifuge；1008th, heater is inactivated；1009th, discarded object passage；1010、
Gloves position.
Embodiment
Below by embodiment, and with reference to accompanying drawing, technical scheme is described in further detail.
Embodiment：A kind of cellular immunotherapy work station of the present invention, as shown in accompanying drawing 1, accompanying drawing 2, it includes：Culture turns
Transport cabin 1002, main operation cabin 1003 and astrodome 1004.
Culture transports cabin 1002, has the CO2gas incubator in CO2gas incubator transfer system 1001
106 are transferred to hatch door and transfer device in cabin（Such as slide rail, automatic sliding device or manipulator）；Work as CO2gas incubator
After transport carriage docks with transhipment cabin, CO2gas incubator is automatically moved into setting position by automatic sliding device or manipulator
Put.When CO2gas incubator needs to be transferred to out of my cabin out of cabin, automatic sliding device or manipulator push away incubator automatically
To incubator transport carriage.Culture transhipment cabin main function is will to need the cell of the Multiplying culture safety inside and outside work station
Transhipment, as transhipment was arrived at a station in the outer time for continuing culture after the completion of the processing of A individual cells, stand in can be for by clearing up sterilizing
The processing of B individual cells；Operating efficiency is substantially increased compared to incubator is fixed in work station.
Main operation cabin 1003, the built-in centrifuge 1007 for centrifugal blood separation and the inactivation for inactivating blood serum are heated
Device 1008, and it is provided with discarded object passage 1009；The operating process of the most cell culture of main operation cabin completion, including cell
Centrifugation, liquid feeding are cultivated, Plasma Inactivation, and the factor is quickly transmitted.
Astrodome 1004, its interior integrating cell viewing microscope, for observing cellular morphology, quantity in cell cultivation process
And pollution condition.
1,004 3 above-mentioned culture transhipment cabin 1002, main operation cabin 1003 and astrodome nacelles are tightly connected into close successively
The working space closed, to form the processing of the immunocyte of closing, culture environment；There is passage able to turn on or off between adjacent nacelle
（Such as hatch door, open and form passage, close separation nacelle）；Main operation cabin 1003 and anterior several hands of installation of astrodome 1004
The gloves position 1010 of set, for operator out of my cabin to being operated manually in cabin.
The side of astrodome 1004 sets main transferring cabin 1005, to transmit follow-up large piece article required in main operation cabin.
Main operation cabin 1003 set several it is quick transmit hatch doors 1006, the quick inner side for transmitting hatch door 1006 is towards main behaviour
Make the operating desk in cabin 1003.The quick hatch door that transmits can complete sterilizing in extremely short speed, instrument that urgent need is used, factor etc.
Article or experiment institute estovers are quickly delivered to main operation platform.
As shown in accompanying drawing 3, accompanying drawing 4, accompanying drawing 5, accompanying drawing 6, accompanying drawing 7, centrifuge includes centrifugation machine liner 12, variable-frequency motor 6
With motor mount 10；Machine liner 12 is centrifuged, its bottom end closure, upper end open, opening portion envelope, which hides centrifuge cup 1 and sets sealing, to be connected
Connect；Built-in horizontal rotor 13 and centrifuge tube adapter 14；Variable-frequency motor 6, it is vertically arranged between centrifugation machine liner 12 bottom, motor shaft end
Bottom wall through centrifugation machine liner 12 enters centrifugal chamber（Centrifuge in machine liner 12）Connect with the horizontal rotor 13, and passing through
Wear portion and sealed connection is set；Motor mount 10, main body are cylinder, and variable-frequency motor 6 is installed at middle part, and end sealing is connected to centrifugation
The bottom of machine liner 12, bottom end seal are connected with access panel 8.Motor mount 10 and connecting portion, the maintenance for centrifuging machine liner 12
The connecting portion of cover plate 8 and motor mount 10 is respectively provided with O-ring seal 4.
In said structure, it is empty that centrifuge cup 1, centrifugation machine liner 12, motor mount 10 and access panel 8 wrap into closing
Between, in the closing space, the centrifugation place space of machine liner 12 is in isolation with the place space of motor mount 10；Isolation
Structure is as follows：The top of variable-frequency motor 6 has boss, and sealing shroud 23 is set on boss；The main body of sealing shroud 23 is the half of bottom opening
Closed cylinder structure, blind end are placed on motor shaft and set outside framework oil seal 3 to be tightly connected to form in joint portion, and openend is outside
Composition annular table is radially extended, the liquid barrier 11 of the elastic construction of annular plate-like is set in annular table and presses the inner edge of liquid barrier 11
Tightly to form the briquetting 26 being tightly connected in annular table, the outer rim of liquid barrier 11 abuts in the centrifugation machine liner of the through-Penetration portion
12 bottom walls are simultaneously compressed to form sealed connection by annular pressing plate 24；Liquid barrier 11 is to deform under stress with pressure interface
Form the elastomer of sealing.First, in this structure, variable-frequency motor 6 is directly entered centrifugation portion and provides centrifugal force, dynamic without other
Power transmission structure, power transmission path reach most short, both improve efficiency of transmission, and have while power is provided for centrifugation portion
Reduce overall volume to effect；Secondly, this structure separates reliable, under the vibrations that variable-frequency motor 6 operates, still can ensure two
Individual space is in segmented state all the time, there is provided good segregation；Further, since liquid barrier 11 is elastomer so that this
Kind structure can facilitate carries out lateral attitude adjustment to variable-frequency motor 6.
The side of centrifuge cup 1 is rotationally connected with centrifugation machine liner 12 by cover hinge 20, the offside of cover hinge 20
The lateral margin of centrifuge cup 1 sets cover handle 17；Cover handle 17 has grip part, and grip part front end extends into card division；Centrifuge
Mechanical lock is set on the top end wall of the opening portion of inner bag 12, the mechanical lock includes lock body and the mechanical dead bolt that can be rotated on lock body
27, lock body bottom outside, which extends laterally, forms hook part 29；When overturning the fastening of centrifuge cup 1, the card division of the cover handle 17
It is pressed against on hook part 29, then rotating machinery dead bolt 27, mechanical dead bolt 27, which is pressed against on card division, forms lock out action.Cover handle 17
Grip part extension card division, mechanical lock and card division coordinate, and changes the open/close states of centrifuge cup 1, card division is not in place then without Vinculum iuris
Fixed, hook part 29, which does not depart from card division, can not then open centrifuge cup 1, the security that the closing lid of centrifuge cup 1 acts significantly is carried
It is high.The mechanical lock structure coordinated with card division is simple, and cost is low, is easy to make, particularly, easy to use, and reliability is high.
To improve the convenience of operation, security and the operating efficiency of complete machine, the card division of cover handle 17 is column, on
Downside is respectively provided with groove, and a sensor 28 is respectively set in two grooves（Such as Hall sensor）；Centrifuge the opening portion of machine liner 12
Gas-flow closure bar 15 is set；During closing lid, the sensor 28 on the downside of card division sends signal, and system is inflated swollen to gas-flow closure bar 15
It is swollen, it is tightly connected with being formed between the centrifugation internal perisporium of machine liner 12 and the lateral wall of centrifuge cup 1；Rotating machinery dead bolt 27 is pressed again
The release mechanism whether started by card division composition variable-frequency motor 6；By the sensor on the upside of card division, into locking state and keep
During locking state, system just opens centrifugal action, once because unusual condition causes to lose locking state, then centrifugal action is stopped automatically
Only, so as to improving security.It is big using the card division intensity of this structure, there is higher bearing capacity, service life is grown, resistance to
Mill, it is not easily damaged.By the sensor on the downside of card division, i.e. inflation of the automatically starting to gas-flow closure bar after the completion of closing lid action
Work, makes centrifuge cup 1 be sealedly attached to centrifugation machine liner；This structure and mode, both make more convenient operation, improve peace again
Quan Xing, it also simplify operation and improve production efficiency.
For the encapsulation scheme of the above-mentioned opening portion of centrifugation machine liner 12, the present invention provides a kind of preferable sealing structure：From
The opening portion periphery of scheming inner bag 12 sets outrigger 31；Outrigger 31 is annular, and section is L-shaped, including transverse frame plate 32 and vertical frame plate
33, transverse frame plate 32 is welded on the centrifugation outer peripheral face of machine liner 12；The top flat of vertical frame plate 33 is equipped with platen 34, and platen 34 is hollow
Ring plate；The horizontal U-shaped frame 30 in opening portion top of the centrifugation machine liner 12, the U-type groove notch of U-shaped frame 30 point to centrifuge
In the middle part of inner bag 12, gas-flow closure bar 15 is set in groove；The outside portion of U-shaped frame 30 connects with the madial wall of vertical frame plate 33, upper lateral part with
The bottom surface of platen 34 connects；Centrifuge cup 1 is double-decker, and the outer peripheral edge of structure on two sides is vertically arranged riser 35；During closing lid, riser 35
Relative with the notch of U-shaped frame 30, gas-flow closure bar 15 is resisted against riser 35 when inflating and is tightly connected with being formed.With outrigger 31, U-shaped
Frame 30, the main body of platen 34 are annular solid, and wherein outrigger 31 is L-type structure, and structural strength is big；The U-shaped downside of frame 30 is horizontally placed on
The opening portion side wall top of machine liner 12 is centrifuged, bottom reclines outrigger 31, and upside reclines platen 34, is provided for gas-flow closure bar 15
Firm sealing supporting structure, support strength is big, is unlikely to deform, and is easy to make, and is easy to seal；Machine liner 12 is centrifuged by above-mentioned
Sealing structure, form good sealing with the riser 35 of centrifuge cup 1 and coordinate；Simultaneously as the superstructure of centrifuge cup 1 is upper
Layer outer rim（In the part in the outside of riser 35）Take and put on platen 34 or the card division of cover handle forms the weight of centrifuge cup 1
Power supporting construction, and hook part 29 forms position limiting structure by the card division of cover handle so that gas-flow closure bar 15 is perpendicular without carrying
To active force, the horizontal force as sealing is only undertaken so that sealing effectiveness is more preferable.
As the main body of drive division, variable-frequency motor 6 is operationally especially also easy to produce vibrations during shift speed change, to examination
Test and have an impact；Therefore, the invention provides a kind of preferably fixed solution of variable-frequency motor 6：The top of variable-frequency motor 6 has disk
21, the bottom of disk 21 is arranged positioning disk 22；It is in ring that positioning disk 22 and the motor mount 10 set several correspondingly
The protruding pin of shape arrangement；On upper and lower corresponding protruding pin, a damping spring 9 is arranged；There is push rod 25, push rod 25 on positioning disk 22
It is resisted against on the annular pressing plate 24 on the centrifugation bottom wall of machine liner 12 of the through-Penetration portion.This fixed structure, it is extremely compact,
It is easily installed, it is fixed reliable, and there is preferable damping effect；Especially, while good damping effect is provided, effectively
Complete machine is maintained less volume range by ground.
The installation portion of protruding pin on motor mount 10 is hierarchic structure, and the small end of stepped end forms spring regulation stud 5,
Spring regulation stud 5 passes through the bottom plate of motor mount 10 and fixed by nut, to finely tune the lateral attitude of variable-frequency motor 6.
This organization plan, using the gap of damping spring and protruding pin（Protruding pin diameter is less than damping spring internal diameter）With protruding pin stepped end
Small end and mounting hole（The end diameter of protruding pin stepped end is less than installation bore dia）Gap, laterally adjust variable-frequency motor 6, from
And the purpose for laterally adjusting position of the horizontal rotor in machine liner is centrifuged is reached；For another angle, by this hair
Bright technical scheme, lateral attitude of the horizontal rotor in centrifugal chamber, can by adjusting the lateral attitude of variable-frequency motor 6,
This is not available for prior art, is one of creative achievement of the present invention.
Protruding pin threaded engagement on positioning disk 22 is fixed, and end of thread extension forms push rod 25, the head of push rod 25 to be spherical or
It is embedded with rotatable spherical.It is easy to remove when installing and adjusting variable-frequency motor 6, avoid damage to the portion with ejector pin contact
Part, especially it is easy for finely tuning the lateral attitude of variable-frequency motor 6.
The bottom of motor mount 10 sets mounting hole and several protruding pins, and the bottom of variable-frequency motor 6 passes from the mounting hole；
The bottom of variable-frequency motor 6 passes from the mounting hole and speed probe 7 is set in the middle part of traversing through end.
As shown in accompanying drawing 8, accompanying drawing 9, CO2gas incubator movement system includes：Upper trolley 103, carbon dioxide gas storage
Tank 104, ups power 105 and stroller 101.
Upper trolley 103, CO2gas incubator 106 is put during for conveying, it includes casing, and bottom half, which is set, to be rolled
Wheel.
Carbon dioxide air accumulator 104, on the casing of upper trolley 103, in conveying to the dioxy in upper trolley
Change carbon incubator 106 to supply, so that CO2gas incubator 106 maintains required internal gas environment.
Ups power 105, on the casing of upper trolley 103, in conveying to the carbon dioxide culture in upper trolley
Case is powered, for CO2gas incubator electrical heating to maintain cultivation temperature.
Stroller 101, top are platform, set guiding upper trolley bottom half roller to slip into upper trolley on platform and put
The upper strata slide rail 102 of position, upper strata slide rail 102 are the trough body structure being adapted to roller, move towards to point to rear end for the front end of stroller；
Using upper strata slide rail, upper trolley and CO2gas incubator are removed or pulled in platform.Stroller is anterior to set guide pad 110,
With it and the service of connection devices（That is main operation cabin）It is affiliated to；When stroller is close to the service of connection devices, guide pad knot corresponding with the service of connection devices
Structure（Such as hole, groove etc.）Coordinate, stroller is entered accurate location, to ensure upper strata slide rail and the extreme pair on the service of connection devices
Just, upper trolley and CO2gas incubator is enable quickly and accurately to be shifted between stroller and the service of connection devices.
In order to further improve docking accuracy of action, prioritization scheme further below can be used, as shown in Figure 9,
Guide pad 110 is two secondary, is split at the anterior both ends of stroller；Guide pad has an open slot, and opening trench bottom is circular arc, notch
For the enlarging extended out；Wherein, the open slot of a secondary guide pad is located at the leading section of guide pad, and the open slot of another guide pad is located at
Inside portion；In the outside of the docking side of the service of connection devices, two leads are set, and lead has cylindrical section, and cylindrical section diameter is with opening
The circular arc bottom adaptation of mouth groove；During docking, by stroller close to the service of connection devices, the guide pad that front end is first set to open slot is caught in correspondingly
Lead, and circular arc bottom is resisted against on the cylindrical section of lead, then centered on lead, promote stroller to rotate
Action, until the open slot of the sidepiece of another guide pad is caught in the cylindrical section of another lead, with complete the positioning of stroller and on
Layer slide rail aligns.This structure, stroller is more accurately entered docking location, align in two perpendicular direction, raising pushes away
The accuracy that upper strata slide rail on car aligns with the extreme on the service of connection devices, make upper trolley and CO2gas incubator
More rapidly, more steadily, the precision for avoiding aligning not enough causes CO2gas incubator to be shaken and shadow in transfer process generation for transfer
Ring the cultivation conditions of the culture in it.
In actual use, because the specification of the service of connection devices is different, the position that stroller is mated with is variant；Therefore, this
Invention provides a preferable technical scheme：The platform on stroller top is mobile platform, moving direction and upper strata guide rail
Guiding direction is substantially the same；The mechanism of driving platform movement includes being arranged on the lower floor's slide rail 109 and screw drives of mesa base
Mechanism.By lower floor's slide rail, platform is slidably fixed on stroller；Operate screw drives mechanism, driving platform movement；Stroller
After being aligned with the service of connection devices, the position of platform can be adjusted in real time by screw drives mechanism, train upper trolley and carbon dioxide
Support case and enter more preferably transferring position.
Culture for cell, tissue and some peculiar microorganisms, external depollution of environment degree is improved, reduced from outer
The pollution in portion, beneficial to the success rate for improving culture；And the box house of upper trolley be not easy it is degerming, need periodically it is degerming；It is even if fixed
When sterilize, be not easy to completely Du absolute external pollution.Therefore, the present invention proposes a preferable solution, by upper strata
The casing of dolly is set to the closed structure that inner space isolates with exterior space；After closed structure, box house and outside
It is completely isolated, without regular sterilization, both completely isolated pollution sources, labor intensity is reduced again.
Further to reduce the source of germ contamination, by carbon dioxide air accumulator 104 in the upper trolley casing
Portion, its tracheae pass through wall box, and the portion of passing sets sealing ring to form and is tightly connected.Ups power is located at the upper trolley case
Internal portion, its cable pass through wall box, and the portion of passing sets sealing ring to form and is tightly connected.By carbon dioxide air accumulator, UPS electricity
Source is placed in the containment housing of upper trolley, completely isolated with exterior space.It should be noted that closed casing is assembled
Structure, for example, the structure with capping, when carbon dioxide air accumulator, ups power are installed on box house and then installation envelope
The joint portion of lid, capping and casing, which is set, to be tightly connected.
When being worked due to ups power, certain heat can be produced, although smaller, in order to ensure the stabilization of long-term work
Property, the present invention proposes a kind of alternative preferred version：The top of the casing of upper trolley has heat insulation structural, prevents heat
Amount is directly passed to CO2gas incubator；The sidepiece of casing or bottom have the heat-conducting part that Heat Conduction Material makes, to pass through heat
Heat in casing is transferred to outside casing by transmission；The hardware for heat conduction, hardware are provided with the outside of ups power
End is connected to the heat-conducting part on the casing of upper trolley.The heat transfer of ups power is led to heat-conducting part by hardware
Hot portion is transferred on the outside of casing, so as to reduce temperature during ups power work.
The CO2gas incubator movement system of the present invention is entered based on basic demand of the user to incubator to existing issue
Row improves, and proposes the technical scheme of the transfer car(buggy) of upper trolley and stroller composition, it is uninterruptedly electric that UPS is equipped with upper trolley
Source, carbon dioxide air accumulator, make CO2gas incubator can continued power, supply in transport process.Upper trolley has timing
Function, start timing when ups power cuts off charging, timing time can be set, the timing time of timer 107 is arrived, and buzzer 108 is reported
Alert prompting charging.In actual use, especially for immunocyte culture longer cycle incubation, effectively
Avoid and CO2gas incubator occur and transport, power off, die（Carbon dioxide）Situation.
With the development of bioscience, the aseptic to cell culture environment and the security requirement to personnel are more and more tighter
Lattice, this also proposes higher requirement to biological plant instrument, and the limitation in traditional GMP laboratories is increasingly difficult to meet newly
The requirement of technology development.Bioscience generates urgent demand to convenient, safe efficient, intelligent biological plant.Therefore,
Present inventors have proposed a kind of technical scheme of cellular immunotherapy work station, is mainly used in the separation and Extraction of cell, induction is lived
Change, culture changes that liquid, passage, collections etc. be multinomial or critical process operation, provide a closed A level environment to test, while can
With integrated multiple function equipment, meet requirement of experiment；Meet bioscience to convenient, safe efficient, intelligent biological plant
Demand.In the cellular immunotherapy work station, the space of closed A level environment is limited, and existing centrifuge volume is big, centrifugation
Easily environment of bacteria growth is formed inside machine, centrifuge internal working volume easily connects with outside experimental enviroment and forms potential sense
Hidden danger is contaminated, therefore, centrifuge scheme of the invention provides a kind of brand-new solution, compact-sized, small volume, suitable for upper
State in the confined space of closed A level environment and use, and overall structure closing, inner space effectively separate, and it is outer to be not easy influence
Portion's experimental enviroment.
Embodiment in specification is only used for that the present invention will be described, and it is not played to protection scope of the present invention
Restriction effect.Protection scope of the present invention is only defined by the claims, and is done on the basis of embodiment disclosed by the invention
Any omission, replacement or modification will fall into protection scope of the present invention.
Claims (10)
- A kind of 1. cellular immunotherapy work station, it is characterised in that including：Culture transports cabin（1002）, have CO2gas incubator transfer system（1001）On CO2gas incubator （106）The hatch door and transfer device being transferred in cabin；Main operation cabin（1003）, the built-in centrifuge for centrifugal blood separation（1007）With the inactivation heating for inactivating blood serum Device（1008）；And astrodome（1004）, its interior integrating cell viewing microscope, for observing cellular morphology, number in cell cultivation process Amount and pollution condition；Wherein, above three nacelle is tightly connected into closed working space successively, to form the processing of the immunocyte of closing, training Support environment；There is passage able to turn on or off between adjacent nacelle；Main operation cabin（1003）And astrodome（1004）Front portion installation is some The gloves position of individual gloves（1010）, for operator out of my cabin to being operated manually in cabin.
- A kind of 2. cellular immunotherapy work station according to claim 1, it is characterised in that：Also include main transferring cabin （1005）, located at astrodome（1004）Side, to transmit follow-up large piece article required in main operation cabin.
- A kind of 3. cellular immunotherapy work station according to claim 1, it is characterised in that：The main operation cabin（1003） Several are set quickly to transmit hatch door（1006）, quickly transmit hatch door（1006）Inner side towards main operation cabin（1003）Operation Platform.
- A kind of 4. cellular immunotherapy work station according to claim 1 or 2 or 3, it is characterised in that：The centrifuge bag Include：Centrifuge machine liner（12）, its bottom end closure, upper end open, opening portion envelope screening centrifuge cup（1）And set sealed connection；It is built-in Horizontal rotor（13）And centrifuge tube adapter（14）；Variable-frequency motor（6）, it is vertically arranged between centrifugation machine liner（12）Bottom, motor shaft end run through the centrifugation machine liner（12）Bottom Wall enters centrifugal chamber and the horizontal rotor（13）Connect, and set and be tightly connected in through-Penetration portion；And motor mount（10）, main body is cylinder, and middle part sets the variable-frequency motor（6）, end sealing be connected to it is described from Scheming inner bag（12）Bottom, bottom end seal are connected with access panel（8）.
- A kind of 5. cellular immunotherapy work station according to claim 4, it is characterised in that：The variable-frequency motor（6）On Portion has boss, and sealing shroud is set on boss（23）；Sealing shroud（23）Main body be bottom opening semiclosed cylindrical structure, closing End is placed on motor shaft and sets outside framework oil seal in joint portion（3）It is tightly connected with forming, openend extends radially outwards composition ring Shape platform, the liquid barrier of the elastic construction of annular plate-like is set in annular table（11）With by liquid barrier（11）Inner edge is pressed on annular table On briquetting（26）, liquid barrier（11）Outer rim abut in the centrifugation machine liner of the through-Penetration portion（12）Bottom wall is simultaneously pressed by annular Plate（24）Compress.
- A kind of 6. cellular immunotherapy work station according to claim 1, it is characterised in that：The centrifuge cup（1）'s Side is rotationally connected with centrifugation machine liner（12）, the centrifuge cup of offside（1）Lateral margin sets cover handle（17）；Cover handle （17）With grip part, grip part front end extends into card division；Centrifuge machine liner（12）Opening portion top end wall on set machinery Lock, the mechanical lock include lock body and the mechanical dead bolt that can be rotated on lock body（27）, lock body bottom outside, which extends laterally, forms hook Portion（29）；When upset centrifuge cup（1）During fastening, the cover handle（17）Card division be pressed against the hook part（29）On, then Rotating machinery dead bolt（27）, mechanical dead bolt（27）It is pressed against on card division and forms lock out action.
- A kind of 7. cellular immunotherapy work station according to claim 6, it is characterised in that：The cover handle（17）'s Card division is column, and upper and lower sides are respectively provided with groove, a sensor is respectively set in two grooves（28）；The centrifugation machine liner（12）Open Oral area sets gas-flow closure bar（15）；During closing lid, the sensor on the downside of card division（28）Signal is sent, system gives gas-flow closure bar （15）Inflation, with centrifugation machine liner（12）Internal perisporium and centrifuge cup（1）Formed and be tightly connected between lateral wall；Revolve again Favourable turn tool dead bolt（27）It is pressed against card division and forms variable-frequency motor（6）The release mechanism whether started.
- A kind of 8. cellular immunotherapy work station according to claim 1 or 2 or 3, it is characterised in that：The carbon dioxide Incubator transfer system（1001）Including：Upper trolley（103）, CO2gas incubator is put during for conveying（106）, it includes casing, and bottom half sets rolling Wheel；Carbon dioxide air accumulator（104）, located at upper trolley（103）Casing on, conveying when to upper trolley（103）On CO2gas incubator（106）Supply, so that CO2gas incubator（106）Internal gas environment needed for maintaining；Ups power（105）, located at upper trolley（103）Casing on, conveying when to upper trolley（103）On titanium dioxide Carbon incubator（106）Power supply, for CO2gas incubator（106）Electrical heating is to maintain cultivation temperature；And stroller（101）, top sets guiding upper trolley（103）Bottom roller slips into the upper strata slide rail of putting position （102）.
- A kind of 9. cellular immunotherapy work station according to claim 8, it is characterised in that：The upper trolley（103） Casing be the closed structure isolated with exterior space of inner space；The ups power（105）Located at the upper trolley （103）Box house, its cable pass through wall box, and the portion of passing sets sealing ring to form and is tightly connected；The upper trolley （103）Casing sidepiece or bottom have Heat Conduction Material make heat-conducting part, with by heat transfer by the heat in casing turn Move to outside casing；The ups power（105）Outside is provided with the hardware for heat conduction, and hardware end is connected to Layer dolly（103）Casing on heat-conducting part.
- A kind of 10. cellular immunotherapy work station according to claim 8, it is characterised in that：The stroller（101）It is anterior Guide pad is set（110）, it is affiliated to it and the service of connection devices；The guide pad（110）It is secondary for two, split in stroller（101）It is anterior Both ends；Guide pad（110）With open slot, opening trench bottom is circular arc, and notch is the enlarging extended out；Wherein, a pair is led To block（110）Open slot be located at guide pad（110）Leading section, another guide pad（110）Open slot be located inside portion； The outside of the docking side of the service of connection devices, sets two leads, lead has the circular arc of cylindrical section, cylindrical section diameter and open slot Shape bottom is adapted to；During docking, by stroller（101）Close to the service of connection devices, the guide pad that front end is first set to open slot is caught in corresponding lead Xiang Zhu, and circular arc bottom is resisted against on the cylindrical section of lead, then centered on lead, promote stroller（101）Turn Action, until another guide pad（110）The open slot of sidepiece be caught in the cylindrical section of another lead, to complete stroller（11） Positioning and upper strata slide rail（102）Align.
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| CN201710862060.8A CN107828650B (en) | 2017-09-21 | 2017-09-21 | Cell immunotherapy workstation |
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Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109439542A (en) * | 2018-10-17 | 2019-03-08 | 深圳市深研生物科技有限公司 | A method of preparing cell |
| CN109486655A (en) * | 2018-12-27 | 2019-03-19 | 英诺维尔智能科技（苏州）有限公司 | A kind of full-automation biological products system |
| CN109943484A (en) * | 2019-04-01 | 2019-06-28 | 上海东富龙医疗装备有限公司 | A kind of RTP carbon dioxide incubator |
| CN110791427A (en) * | 2019-10-25 | 2020-02-14 | 上海东富龙医疗装备有限公司 | General cell processing total station system |
| CN111334429A (en) * | 2020-03-25 | 2020-06-26 | 北京双诚联盈净化工程技术有限公司 | Modular multifunctional cell workstation |
| CN113621568A (en) * | 2021-07-22 | 2021-11-09 | 江苏蒙彼利生物科技有限公司 | Umbilical cord mesenchymal stem cell serum-free culture equipment and culture method thereof |
Citations (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN2040004U (en) * | 1988-07-14 | 1989-06-28 | 西安第四军医大学 | Multi-functional digital display table type high-speed centrifuge |
| CN1453352A (en) * | 2002-04-26 | 2003-11-05 | 叶大林 | Bacteria-free isolated operation system |
| CN201143275Y (en) * | 2008-01-04 | 2008-11-05 | 陈再宏 | Incubator for transporting baby |
| CN105252242A (en) * | 2015-09-06 | 2016-01-20 | 吕亮霞 | Movement and pose adjusting platform for large member |
| CN205926052U (en) * | 2016-08-02 | 2017-02-08 | 义和诚集团有限公司 | Glass is centrifuge for liquid |
| CN205969130U (en) * | 2016-08-30 | 2017-02-22 | 浙江泰林生物技术股份有限公司 | Isolator system is used in allotment of cytotoxicity vein medicine |
| US20170130189A1 (en) * | 2014-06-30 | 2017-05-11 | Shibuya Corporation | Automatic culturing device |
| CN106694243A (en) * | 2015-11-12 | 2017-05-24 | 上海安亭科学仪器厂 | Special PRP centrifuge |
| EP3199614A1 (en) * | 2014-09-26 | 2017-08-02 | Public University Corporation Yokohama City University | Cell culture system |
| CN206444737U (en) * | 2017-01-20 | 2017-08-29 | 苏州星亿机械有限公司 | special high-performance centrifuge |
| CN207498394U (en) * | 2017-09-21 | 2018-06-15 | 浙江泰林生物技术股份有限公司 | A kind of cellular immunotherapy work station |
-
2017
- 2017-09-21 CN CN201710862060.8A patent/CN107828650B/en active Active
Patent Citations (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN2040004U (en) * | 1988-07-14 | 1989-06-28 | 西安第四军医大学 | Multi-functional digital display table type high-speed centrifuge |
| CN1453352A (en) * | 2002-04-26 | 2003-11-05 | 叶大林 | Bacteria-free isolated operation system |
| CN201143275Y (en) * | 2008-01-04 | 2008-11-05 | 陈再宏 | Incubator for transporting baby |
| US20170130189A1 (en) * | 2014-06-30 | 2017-05-11 | Shibuya Corporation | Automatic culturing device |
| EP3199614A1 (en) * | 2014-09-26 | 2017-08-02 | Public University Corporation Yokohama City University | Cell culture system |
| CN105252242A (en) * | 2015-09-06 | 2016-01-20 | 吕亮霞 | Movement and pose adjusting platform for large member |
| CN106694243A (en) * | 2015-11-12 | 2017-05-24 | 上海安亭科学仪器厂 | Special PRP centrifuge |
| CN205926052U (en) * | 2016-08-02 | 2017-02-08 | 义和诚集团有限公司 | Glass is centrifuge for liquid |
| CN205969130U (en) * | 2016-08-30 | 2017-02-22 | 浙江泰林生物技术股份有限公司 | Isolator system is used in allotment of cytotoxicity vein medicine |
| CN206444737U (en) * | 2017-01-20 | 2017-08-29 | 苏州星亿机械有限公司 | special high-performance centrifuge |
| CN207498394U (en) * | 2017-09-21 | 2018-06-15 | 浙江泰林生物技术股份有限公司 | A kind of cellular immunotherapy work station |
Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109439542A (en) * | 2018-10-17 | 2019-03-08 | 深圳市深研生物科技有限公司 | A method of preparing cell |
| CN109486655A (en) * | 2018-12-27 | 2019-03-19 | 英诺维尔智能科技（苏州）有限公司 | A kind of full-automation biological products system |
| CN109943484A (en) * | 2019-04-01 | 2019-06-28 | 上海东富龙医疗装备有限公司 | A kind of RTP carbon dioxide incubator |
| CN110791427A (en) * | 2019-10-25 | 2020-02-14 | 上海东富龙医疗装备有限公司 | General cell processing total station system |
| CN111334429A (en) * | 2020-03-25 | 2020-06-26 | 北京双诚联盈净化工程技术有限公司 | Modular multifunctional cell workstation |
| CN113621568A (en) * | 2021-07-22 | 2021-11-09 | 江苏蒙彼利生物科技有限公司 | Umbilical cord mesenchymal stem cell serum-free culture equipment and culture method thereof |
| CN113621568B (en) * | 2021-07-22 | 2024-02-13 | 江苏蒙彼利生物科技有限公司 | Umbilical cord mesenchymal stem cell serum-free culture equipment and culture method thereof |
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