CN107649296A

CN107649296A - A kind of built-in centrifugal machine

Info

Publication number: CN107649296A
Application number: CN201710862383.7A
Authority: CN
Inventors: 叶大林; 夏信群
Original assignee: Zhejiang Tailin Bioengineering Co Ltd
Current assignee: Zhejiang Tailin Bioengineering Co Ltd
Priority date: 2017-09-21
Filing date: 2017-09-21
Publication date: 2018-02-02
Anticipated expiration: 2037-09-21
Also published as: CN107649296B

Abstract

The present invention relates to a kind of built-in centrifugal machine, it includes：Machine liner is centrifuged, its bottom end closure, upper end open, opening portion seals screening centrifuge cup and simultaneously sets sealed connection；Built-in horizontal rotor and centrifuge tube adapter；Variable-frequency motor, centrifugation machine liner bottom is vertically arranged between, motor shaft end connects through the bottom wall of the centrifugation machine liner into centrifugal chamber with the horizontal rotor, and sets and be tightly connected in through-Penetration portion；And motor mount, main body are cylinder, middle part sets the variable-frequency motor, and end sealing is connected to centrifuge liner at the bottom, and bottom end seal is connected with access panel.Above-mentioned three parts are sequentially overlapped composition from top to bottom, compact-sized, and small volume, working space occupancy are small, suitable for being used in less test space；And completely enclosed structure is wrapped, while inside is divided into two not connected spaces, has both been not easy, by external environment condition infection centrifugation portion, to be not easy internally to form environment of bacteria growth and infect outside test space again.

Description

A kind of built-in centrifugal machine

Technical field

The present invention relates to a kind of Bioexperiment equipment, more particularly, to a kind of built-in centrifugal machine.

Background technology

The centrifuge for being presently used for biological cell separation is all desk centrifuge, mostly independent body, such as entitled " biological safety centrifugal machine "（The B of notification number CN 101767066）, entitled " a kind of biological centrifuge "（Publication No. CN 104084319 A）, entitled " a kind of constant temperature oscillation centrifuge "（The U of notification number CN 202555409）Deng shown in public technology Structure；Prior art including technology disclosed above, volume big, inner bag blow-by be present, without communication interface the defects of, It is difficult to use in less insulating space and uses, especially centrifuge itself easily turns into environment of bacteria growth, in insulating space Carry out easily forming infection during the experiment such as Bacteria Culture, interference test can influence the accuracy of experiment when serious, or even hinder experiment Be normally carried out.

The content of the invention

Present invention is primarily intended to provide a kind of built-in centrifugal machine, its small volume, the proving ring limited available for space In border；In the experiment of closing space, test for contamination environment is not easy.

The above-mentioned technical problem of the present invention is mainly what is be addressed by following technical proposals：A kind of built-in centrifugal Machine, it includes：

Machine liner is centrifuged, its bottom end closure, upper end open, opening portion seals screening centrifuge cup and simultaneously sets sealed connection；Built-in horizontal turn Son and centrifuge tube adapter；

Variable-frequency motor, is vertically arranged between centrifugation machine liner bottom, and motor shaft end enters centrifugation through the bottom wall of the centrifugation machine liner Room connects with the horizontal rotor, and sets and be tightly connected in through-Penetration portion；

And motor mount, main body are cylinder, middle part sets the variable-frequency motor, and end sealing is connected to centrifugation machine liner bottom Portion, bottom end seal are connected with access panel.

Pass through above-mentioned technical proposal, in the present invention, centrifugation machine liner, variable-frequency motor and motor mount three parts from upper and Under be sequentially overlapped composition, it is compact-sized, small volume, working space take it is small, suitable for being used in less test space；Especially It is this structure, centrifuge cup, centrifugation machine liner, motor mount wrap into completely enclosed structure with access panel, simultaneously Inside is divided into two not connected spaces so that in closing lid, inside can be completely separated with outside, had both been not easy by external rings Border infection centrifugation portion, is not easy internally to form environment of bacteria growth and infect outside test space again.

Brief description of the drawings

Accompanying drawing 1 is a kind of structural representation of the present invention.

Accompanying drawing 2 is the top view of accompanying drawing 1.

Accompanying drawing 3 is enlarged drawing at the A of accompanying drawing 1.

Accompanying drawing 4 is the axonometric drawing of the present invention.

Accompanying drawing 5 is a kind of structural representation for centrifuging machine liner opening portion.

Wherein：1st, centrifuge cup；2nd, abutting joint face；3rd, outside framework oil seal；4th, O-ring seal；5th, spring regulation stud；6、 Variable-frequency motor；7th, speed probe；8th, access panel；9th, damping spring；10th, motor mount；11st, liquid barrier；12nd, centrifuge Inner bag；13rd, horizontal rotor；14th, centrifuge tube adapter；15th, gas-flow closure bar；16th, hood lock；17th, cover handle；18th, touch Screen；19th, safety glass form；20th, cover hinge；21st, disk；22nd, positioning disk；23rd, sealing shroud；24th, annular pressing plate；25th, push up Bar；26th, briquetting；27th, mechanical dead bolt；28th, sensor；29th, hook part；30th, U-shaped frame；31st, outrigger；32nd, transverse frame plate；33rd, vertical frame Plate；34th, platen；35th, riser.

Embodiment

Below by embodiment, and with reference to accompanying drawing, technical scheme is described in further detail.

Embodiment：A kind of built-in centrifugal machine of the present invention, as shown in accompanying drawing 1, accompanying drawing 2, accompanying drawing 3, accompanying drawing 4, accompanying drawing 5, its Including centrifugation machine liner 12, variable-frequency motor 6 and motor mount 10；Machine liner 12 is centrifuged, its bottom end closure, upper end open, is opened Oral area envelope hides centrifuge cup 1 and sets sealed connection；Built-in horizontal rotor 13 and centrifuge tube adapter 14；Variable-frequency motor 6, is vertically arranged between The bottom of machine liner 12 is centrifuged, motor shaft end enters centrifugal chamber through the bottom wall of centrifugation machine liner 12（Centrifuge in machine liner 12） Connect with the horizontal rotor 13, and set and be tightly connected in through-Penetration portion；Motor mount 10, main body are cylinder, and middle part is installed Variable-frequency motor 6, end sealing are connected to centrifugation machine liner 12 bottom, and bottom end seal is connected with access panel 8.Motor mount 10 With centrifuging the connecting portion of machine liner 12, the connecting portion of access panel 8 and motor mount 10 is respectively provided with O-ring seal 4.

In said structure, it is empty that centrifuge cup 1, centrifugation machine liner 12, motor mount 10 and access panel 8 wrap into closing Between, in the closing space, the centrifugation place space of machine liner 12 is in isolation with the place space of motor mount 10；Isolation Structure is as follows：The top of variable-frequency motor 6 has boss, and sealing shroud 23 is set on boss；The main body of sealing shroud 23 is the half of bottom opening Closed cylinder structure, blind end are placed on motor shaft and set outside framework oil seal 3 to be tightly connected to form in joint portion, and openend is outside Composition annular table is radially extended, the liquid barrier 11 of the elastic construction of annular plate-like is set in annular table and presses the inner edge of liquid barrier 11 Tightly to form the briquetting 26 being tightly connected in annular table, the outer rim of liquid barrier 11 abuts in the centrifugation machine liner of the through-Penetration portion 12 bottom walls are simultaneously compressed to form sealed connection by annular pressing plate 24；Liquid barrier 11 is to deform under stress with pressure interface Form the elastomer of sealing.First, in this structure, variable-frequency motor 6 is directly entered centrifugation portion and provides centrifugal force, dynamic without other Power transmission structure, power transmission path reach most short, both improve efficiency of transmission, and have while power is provided for centrifugation portion Reduce overall volume to effect；Secondly, this structure separates reliable, under the vibrations that variable-frequency motor 6 operates, still can ensure two Individual space is in segmented state all the time, there is provided good segregation；Further, since liquid barrier 11 is elastomer so that this Kind structure can facilitate carries out lateral attitude adjustment to variable-frequency motor 6.

The side of centrifuge cup 1 is rotationally connected with centrifugation machine liner 12 by cover hinge 20, the offside of cover hinge 20 The lateral margin of centrifuge cup 1 sets cover handle 17；Cover handle 17 has grip part, and grip part front end extends into card division；Centrifuge Mechanical lock is set on the top end wall of the opening portion of inner bag 12, the mechanical lock includes lock body and the mechanical dead bolt that can be rotated on lock body 27, lock body bottom outside, which extends laterally, forms hook part 29；When overturning the fastening of centrifuge cup 1, the card division of the cover handle 17 It is pressed against on hook part 29, then rotating machinery dead bolt 27, mechanical dead bolt 27, which is pressed against on card division, forms lock out action.Cover handle 17 Grip part extension card division, mechanical lock and card division coordinate, and changes the open/close states of centrifuge cup 1, card division is not in place then without Vinculum iuris Fixed, hook part 29, which does not depart from card division, can not then open centrifuge cup 1, the security that the closing lid of centrifuge cup 1 acts significantly is carried It is high.The mechanical lock structure coordinated with card division is simple, and cost is low, is easy to make, particularly, easy to use, and reliability is high.

To improve the convenience of operation, security and the operating efficiency of complete machine, the card division of cover handle 17 is column, on Downside is respectively provided with groove, and a sensor 28 is respectively set in two grooves（Such as Hall sensor）；Centrifuge the opening portion of machine liner 12 Gas-flow closure bar 15 is set；During closing lid, the sensor 28 on the downside of card division sends signal, and system is inflated swollen to gas-flow closure bar 15 It is swollen, it is tightly connected with being formed between the centrifugation internal perisporium of machine liner 12 and the lateral wall of centrifuge cup 1；Rotating machinery dead bolt 27 is pressed again The release mechanism whether started by card division composition variable-frequency motor 6；By the sensor on the upside of card division, into locking state and keep During locking state, system just opens centrifugal action, once because unusual condition causes to lose locking state, then centrifugal action is stopped automatically Only, so as to improving security.It is big using the card division intensity of this structure, there is higher bearing capacity, service life is grown, resistance to Mill, it is not easily damaged.By the sensor on the downside of card division, i.e. inflation of the automatically starting to gas-flow closure bar after the completion of closing lid action Work, makes centrifuge cup 1 be sealedly attached to centrifugation machine liner；This structure and mode, both make more convenient operation, improve peace again Quan Xing, it also simplify operation and improve production efficiency.

For the encapsulation scheme of the above-mentioned opening portion of centrifugation machine liner 12, the present invention provides a kind of preferable sealing structure：From The opening portion periphery of scheming inner bag 12 sets outrigger 31；Outrigger 31 is annular, and section is L-shaped, including transverse frame plate 32 and vertical frame plate 33, transverse frame plate 32 is welded on the centrifugation outer peripheral face of machine liner 12；The top flat of vertical frame plate 33 is equipped with platen 34, and platen 34 is hollow Ring plate；The horizontal U-shaped frame 30 in opening portion top of the centrifugation machine liner 12, the U-type groove notch of U-shaped frame 30 point to centrifuge In the middle part of inner bag 12, gas-flow closure bar 15 is set in groove；The outside portion of U-shaped frame 30 connects with the madial wall of vertical frame plate 33, upper lateral part with The bottom surface of platen 34 connects；Centrifuge cup 1 is double-decker, and the outer peripheral edge of structure on two sides is vertically arranged riser 35；During closing lid, riser 35 Relative with the notch of U-shaped frame 30, gas-flow closure bar 15 is resisted against riser 35 when inflating and is tightly connected with being formed.With outrigger 31, U-shaped Frame 30, the main body of platen 34 are annular solid, and wherein outrigger 31 is L-type structure, and structural strength is big；The U-shaped downside of frame 30 is horizontally placed on The opening portion side wall top of machine liner 12 is centrifuged, bottom reclines outrigger 31, and upside reclines platen 34, is provided for gas-flow closure bar 15 Firm sealing supporting structure, support strength is big, is unlikely to deform, and is easy to make, and is easy to seal；Machine liner 12 is centrifuged by above-mentioned Sealing structure, form good sealing with the riser 35 of centrifuge cup 1 and coordinate；Simultaneously as the superstructure of centrifuge cup 1 is upper Layer outer rim（In the part in the outside of riser 35）Take and put on platen 34 or the card division of cover handle forms the weight of centrifuge cup 1 Power supporting construction, and hook part 29 forms position limiting structure by the card division of cover handle so that gas-flow closure bar 15 is perpendicular without carrying To active force, the horizontal force as sealing is only undertaken so that sealing effectiveness is more preferable.

As the main body of drive division, variable-frequency motor 6 is operationally especially also easy to produce vibrations during shift speed change, to examination Test and have an impact；Therefore, the invention provides a kind of preferably fixed solution of variable-frequency motor 6：The top of variable-frequency motor 6 has disk 21, the bottom of disk 21 is arranged positioning disk 22；It is in ring that positioning disk 22 and the motor mount 10 set several correspondingly The protruding pin of shape arrangement；On upper and lower corresponding protruding pin, a damping spring 9 is arranged；There is push rod 25, push rod 25 on positioning disk 22 It is resisted against on the annular pressing plate 24 on the centrifugation bottom wall of machine liner 12 of the through-Penetration portion.This fixed structure, it is extremely compact, It is easily installed, it is fixed reliable, and there is preferable damping effect；Especially, while good damping effect is provided, effectively Complete machine is maintained less volume range by ground.

The installation portion of protruding pin on motor mount 10 is hierarchic structure, and the small end of stepped end forms spring regulation stud 5, Spring regulation stud 5 passes through the bottom plate of motor mount 10 and fixed by nut, to finely tune the lateral attitude of variable-frequency motor 6. This organization plan, using the gap of damping spring and protruding pin（Protruding pin diameter is less than damping spring internal diameter）, protruding pin stepped end Small end and mounting hole（The end diameter of protruding pin stepped end is less than installation bore dia）Gap, laterally adjust variable-frequency motor 6, from And the purpose for laterally adjusting position of the horizontal rotor in machine liner is centrifuged is reached；For another angle, by this hair Bright technical scheme, lateral attitude of the horizontal rotor in centrifugal chamber, can by adjusting the lateral attitude of variable-frequency motor 6, This is not available for prior art, is one of creative achievement of the present invention.

Protruding pin threaded engagement on positioning disk 22 is fixed, and end of thread extension forms push rod 25, the head of push rod 25 to be spherical or It is embedded with rotatable spherical.It is easy to remove when installing and adjusting variable-frequency motor 6, avoid damage to the portion with ejector pin contact Part, especially it is easy for finely tuning the lateral attitude of variable-frequency motor 6.

The bottom of motor mount 10 sets mounting hole and several protruding pins, and the bottom of variable-frequency motor 6 passes from the mounting hole； The bottom of variable-frequency motor 6 passes from the mounting hole and speed probe 7 is set in the middle part of traversing through end.

With the development of bioscience, the aseptic to cell culture environment and the security requirement to personnel are more and more tighter Lattice, this also proposes higher requirement to biological plant instrument, and the limitation in traditional GMP laboratories is increasingly difficult to meet newly The requirement of technology development.Bioscience generates urgent demand to convenient, safe efficient, intelligent biological plant.Therefore, Present inventors have proposed a kind of cellular immunotherapy work station, it is mainly used in the separation and Extraction of cell, induced activation, culture are changed Liquid, passage, collection etc. are multinomial or critical process operates, and provide a closed A level environment for experiment, while can integrate multinomial Function device, meet that operation requires.In the cellular immunotherapy work station, the space of closed A level environment is limited, existing Centrifuge volume is big, environment of bacteria growth, centrifuge internal working volume are easily formed inside centrifuge easily with outside experimental enviroment Connect and form potential infection hidden danger, therefore, the invention provides a kind of brand-new solution, compact-sized, small volume, Used in the confined space suitable for above-mentioned closed A level environment, and overall structure closing, inner space effectively separate, and are not easy Influence outside experimental enviroment.

It is presently believed to be most practical and preferred embodiment although combined invention has been described, can With understanding, the invention is not limited in disclosed embodiment and element, on the contrary, it is intended to cover being included in appended power Combination, equivalent device and the equivalent element of various remodeling, feature within the spirit and scope of sharp claim.Therefore, The present invention be used for cover to the present invention remodeling and deformation, as long as they appended claims and they wait efficacious prescriptions Within the scope of case.

Claims

A kind of 1. built-in centrifugal machine, it is characterised in that including：

Centrifuge machine liner（12）, its bottom end closure, upper end open, opening portion envelope screening centrifuge cup（1）And set sealed connection；It is built-in Horizontal rotor（13）And centrifuge tube adapter（14）；

Variable-frequency motor（6）, it is vertically arranged between centrifugation machine liner（12）Bottom, motor shaft end run through the centrifugation machine liner（12）Bottom Wall enters centrifugal chamber and the horizontal rotor（13）Connect, and set and be tightly connected in through-Penetration portion；

And motor mount（10）, main body is cylinder, and middle part sets the variable-frequency motor（6）, end sealing be connected to it is described from Scheming inner bag（12）Bottom, bottom end seal are connected with access panel（8）.
A kind of 2. built-in centrifugal machine according to claim 1, it is characterised in that：The variable-frequency motor（6）Top has Boss, sealing shroud is set on boss（23）；Sealing shroud（23）Main body be bottom opening semiclosed cylindrical structure, blind end is placed on Motor shaft simultaneously sets outside framework oil seal in joint portion（3）It is tightly connected with forming, openend extends radially outwards composition annular table, ring The liquid barrier of the elastic construction of annular plate-like is set on shape platform（11）With by liquid barrier（11）Inner edge is pressed on the pressure in annular table Block（26）, liquid barrier（11）Outer rim abut in the centrifugation machine liner of the through-Penetration portion（12）Bottom wall simultaneously passes through annular pressing plate（24） Compress.
A kind of 3. built-in centrifugal machine according to claim 1, it is characterised in that：The centrifuge cup（1）Side turn It is dynamic to be connected to centrifugation machine liner（12）, the centrifuge cup of offside（1）Lateral margin sets cover handle（17）；Cover handle（17）Have Grip part, grip part front end extend into card division；Centrifuge machine liner（12）Opening portion top end wall on mechanical lock is set, the machinery Lock includes lock body and the mechanical dead bolt that can be rotated on lock body（27）, lock body bottom outside, which extends laterally, forms hook part（29）；When Overturn centrifuge cup（1）During fastening, the cover handle（17）Card division be pressed against the hook part（29）On, then rotating machinery lock Tongue（27）, mechanical dead bolt（27）It is pressed against on card division and forms lock out action.
A kind of 4. built-in centrifugal machine according to claim 3, it is characterised in that：The cover handle（17）Card division be Column, upper and lower sides are respectively provided with groove, a sensor are respectively set in two grooves（28）；The centrifugation machine liner（12）Opening portion set Put gas-flow closure bar（15）；During closing lid, the sensor on the downside of card division（28）Signal is sent, system gives gas-flow closure bar（15）Inflation Expansion, with centrifugation machine liner（12）Internal perisporium and centrifuge cup（1）Formed and be tightly connected between lateral wall；Rotating machinery is locked again Tongue（27）It is pressed against card division and forms variable-frequency motor（6）The release mechanism whether started.
A kind of 5. built-in centrifugal machine according to claim 4, it is characterised in that：The centrifugation machine liner（12）Opening Portion periphery sets outrigger（31）；Outrigger（31）For annular, section is L-shaped, including transverse frame plate（32）With vertical frame plate（33）, transverse frame plate （32）It is welded on centrifugation machine liner（12）On outer peripheral face；Vertical frame plate（33）Top flat is equipped with platen（34）, platen（34）To be hollow Ring plate；The centrifugation machine liner（12）The horizontal U-shaped frame in opening portion top（30）, U-shaped frame（30）U-type groove notch refer to To centrifugation machine liner（12）Middle part, gas-flow closure bar is set in groove（15）；U-shaped frame（30）Outside portion and vertical frame plate（33）Inner side Wall connects, upper lateral part and platen（34）Bottom surface connect；The centrifuge cup（1）For double-decker, the outer peripheral edge of structure on two sides It is vertically arranged riser（35）；During closing lid, riser（35）With U-shaped frame（30）Notch it is relative, gas-flow closure bar（15）It is resisted against during inflation Riser（35）It is tightly connected with being formed.
A kind of 6. built-in centrifugal machine according to claim 1, it is characterised in that：The motor mount（10）With it is described Centrifuge machine liner（12）Connecting portion, the access panel（8）With the motor mount（10）Connecting portion be respectively provided with it is O-shaped close Seal（4）.
A kind of 7. built-in centrifugal machine according to claim 1 or 2 or 3 or 4 or 5 or 6, it is characterised in that：The frequency conversion Motor（6）Top has disk（21）, disk（21）Bottom is arranged positioning disk（22）；Positioning disk（22）With the motor mount （10）Several protruding pins arranged in a ring are set correspondingly；On upper and lower corresponding protruding pin, a damping spring is arranged （9）；Positioning disk（22）It is upper that there is push rod（25）, push rod（25）It is resisted against the centrifugation machine liner of the through-Penetration portion（12）On bottom wall Annular pressing plate（24）On.
A kind of 8. built-in centrifugal machine according to claim 7, it is characterised in that：The motor mount（10）On it is convex The installation portion of pin is hierarchic structure, and the small end of stepped end forms spring regulation stud（5）, spring regulation stud（5）Through motor Mounting seat（10）Bottom plate and fixed by nut, to finely tune the variable-frequency motor（6）Lateral attitude.
A kind of 9. built-in centrifugal machine according to claim 7, it is characterised in that：The positioning disk（22）On protruding pin lead to Cross threaded engagement to fix, end of thread extension forms push rod（25）, push rod（25）Head is spherical or is embedded with rotatable spherical Body.
A kind of 10. built-in centrifugal machine according to claim 7, it is characterised in that：The motor mount（10）Bottom Portion sets mounting hole, the variable-frequency motor（6）Bottom passed from the mounting hole and speed probe set in the middle part of traversing through end（7）.