CN107823117A - A kind of anti-aging skin care product containing sealwort stem cell extract - Google Patents
A kind of anti-aging skin care product containing sealwort stem cell extract Download PDFInfo
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- CN107823117A CN107823117A CN201711312847.3A CN201711312847A CN107823117A CN 107823117 A CN107823117 A CN 107823117A CN 201711312847 A CN201711312847 A CN 201711312847A CN 107823117 A CN107823117 A CN 107823117A
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
- A61K8/99—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from microorganisms other than algae or fungi, e.g. protozoa or bacteria
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
- A61K8/97—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
- A61K8/98—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution of animal origin
- A61K8/987—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution of animal origin of species other than mammals or birds
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q17/00—Barrier preparations; Preparations brought into direct contact with the skin for affording protection against external influences, e.g. sunlight, X-rays or other harmful rays, corrosive materials, bacteria or insect stings
- A61Q17/005—Antimicrobial preparations
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
- A61Q19/08—Anti-ageing preparations
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
- A61K2800/40—Chemical, physico-chemical or functional or structural properties of particular ingredients
- A61K2800/59—Mixtures
- A61K2800/592—Mixtures of compounds complementing their respective functions
- A61K2800/5922—At least two compounds being classified in the same subclass of A61K8/18
Abstract
The invention discloses a kind of anti-apolexis composition, the composition of following parts by weight is included in the anti-apolexis composition：0.77~8.9 part of 0.04~3.7 part of sealwort stem cell extract, 0.6~7.5 part of echinacea pallida extract, 0.8~8.3 part of silk-extract, 1.1~9.2 parts of bigcatkin willow extract and spikemoss extract.Meanwhile the invention also discloses the skin care item containing above-mentioned anti-apolexis composition.Skin care item in the present invention include sealwort stem cell extract, echinacea pallida extract, silk-extract, bigcatkin willow extract and spikemoss extract, nutriment in these extracts is combined can effective delaying skin aging, suppress bacterial reproduction simultaneously, and, it is useful and harmless to skin without preservative.
Description
Technical field
The present invention relates to a kind of anti-aging skin care item, especially a kind of anti-aging shield containing sealwort stem cell extract
Skin product.
Background technology
Skin aging is the skin senescence phenomenon as caused by natural cause or non-natural factor.Skin histology day after people's birth
Reach increasingly, function is gradually active, will start to degenerate when reaching certain age, and this degeneration is often unconsciously slow in people
Slow-motion row.The growth stage of skin histology typically ends at 25 years old or so, and it is " bent angle of skin " to have this phase of person, hereafter grows certainly
Carried out simultaneously with aging, Elastic fibers are gradually thicker.
Skin ageing phenomenon is mainly manifested in two aspects：
1st, skin histology fails.The thickness of skin has obvious change with the increase at age.During the epidermis of people 20 years old most
Thickness, later gradually it is thinning, to senescence phase stratum granulosum can atrophy to disappear, prickle cell's short survival.Epidermal cell nuclear fission increases
Add, therefore melanin also increases, so that the colour of skin of the elderly is mostly brownish black.Because aged cells are attached to horny layer of epidermis, make
Skin surface is hardened, and tarnishes.Corium is most thick at 30 years old, and later gradual change is thin and with atrophy.Subcutaneous fat is reduced, and by
Changed in elastic fibers and collagenous fibres and gradually lose skin elasticity and tension force, further cause cutis laxa to be produced with wrinkle
It is raw；
2nd, physiological function is low.Sebaceous glands, sweat gland deterioration, sweat exclude to reduce with sebum, and skin gradually loses former times
Day gloss and become drying.Blood circulation hypofunction is difficult to supplement the necessary nutrition of skin, therefore the elderly's skin wound is difficult
Healing.
On October 25th, 2010, Nature Biotechnology have delivered the research institute of the Unhwa Corp companies of South Korea
It is successfully separated first and has cultivated the paper of plant stem cell, and certification has been obtained to the identity of plant stem cell, plant does
The research of cell enters the stage of high speed development.
In simple terms, there are two " group " cells for being located at plant tip in plant, they have powerful self-renewing
Ability, it can not only be divided into autogenous cell, moreover it is possible to other any plant cells are divided into, are qualified " general-purpose cells ",
Moreover, they, which also have, continues splitting ability so that many large-scale plant existences more than thousand.
2012, " plant stem cell " technology was studied in the effect of beauty and health care field and has been achieved for certain progress,
Metabolism of the plant stem cell to raising skin is proved, improves microcirculation, tightening up and recovering skin elasticity has major application
Value.
The content of the invention
Based on this, one kind is provided and can effectively be delayed it is an object of the invention to overcome above-mentioned the deficiencies in the prior art part
The skin care item of aging.
To achieve the above object, the technical scheme taken of the present invention is：
A kind of anti-apolexis composition, the composition of following parts by weight is included in the anti-apolexis composition：Sealwort stem cell carries
Take 0.04~3.7 part of thing, 0.6~7.5 part of echinacea pallida extract, 0.8~8.3 part of silk-extract, bigcatkin willow extract 1.1
~9.2 parts and 0.77~8.9 part of spikemoss extract.
Wherein, sealwort stem cell can play interference effect by epigenetic factor pair human skin cell, can be effective
The power of regeneration of skin progenitor cell is encouraged, hyperplasia fibrillar structure albumen, accelerates self more raw and reparation of skin；In addition, sealwort has
There are boosting qi and nourishing yin, invigorating the spleen, moistening lung, the effect of kidney-nourishing；Echinacea purpurea is generally considered, with immunological enhancement, to contain various active
Composition, the immunocyte vigor such as leucocyte in human body can be stimulated, improve body autoimmunity；Modern medical experiment proves, silkworm
The protein content of silk is much higher than pearl, and wherein nitrogen content is higher than pearl 37 times, and primary amino acid content is up to more than 10 times,
These amino acid can directly be human hair, and skin absorbs and absorption, i.e., be easier to permeate in the outer layer of human epidermal, accelerate skin
The metabolism of skin；Silk contracting amino acid can also effectively suppress the generation of melanin in skin；There is medical book record, use bitter willow branchlet and leaf
Five jin, decoct soup warm bath, water cooling heat exchange, the gradual row slurry of variola passes through completely, such as discontented, can bathe several times, power weak person, face hand of only washing hair more
Foot, for oral administration to help qi and blood medicine, acne poison is not sent out, and effect is good；100% Selaginella tamariscina decoction is burrowed method with flat board, is had to staphylococcus aureus
Inhibitory action.
Preferably, the mass ratio of the sealwort stem cell extract, echinacea pallida extract and silk-extract is
0.09~0.75:3.1:2~5.3.It is highly preferred that sealwort stem cell extract, echinacea pallida extract and the silk carry
The mass ratio for taking thing is 0.35:3:4.1.Present inventor has found through test of many times, when sealwort stem cell carries in skin care item
The mass ratio for taking thing, echinacea pallida extract and silk-extract is 0.09~0.75:3.1:When 2~5.3, skin care item resist
Aging effects are preferable；And when the quality of sealwort stem cell extract, echinacea pallida extract and silk-extract in skin care item
Than for 0.35:3:When 4.1, the anti-aging effects of skin care item are more preferably.
Preferably, the mass ratio of the sealwort stem cell extract and spikemoss extract is 0.11~0.68:4.7.It is more excellent
Selection of land, the mass ratio of the sealwort stem cell extract and spikemoss extract is 0.32:4.7.Present inventor is through multiple
Experiment is found, when the mass ratio of sealwort stem cell extract and spikemoss extract is 0.11~0.68 in skin care item:When 4.7, shield
The fungistatic effect of skin product is preferable；And it is 0.32 to work as the mass ratio of sealwort stem cell extract and spikemoss extract in skin care item:4.7
When, the fungistatic effect of skin care item is more preferably.
Preferably, the composition of following parts by weight is included in the anti-apolexis composition：0.35 part of sealwort stem cell extract,
5.14 parts of 3 parts of echinacea pallida extract, 4.1 parts of silk-extract, 7.9 parts of bigcatkin willow extract and spikemoss extract.The application
Inventor found through test of many times, when in skin care item contain 0.35 part of sealwort stem cell extract, echinacea pallida extract 3
When part, 4.1 parts of silk-extract, 7.9 parts of bigcatkin willow extract and 5.14 parts of spikemoss extract, the anti-aging effects of skin care item and suppression
Bacterium effect is all good.
As the second aspect of the invention, the invention provides a kind of anti-aging skin care product, the skin care item include upper
The composition stated.
Preferably, sealwort stem cell extract, echinacea pallida extract, silk-extract, bigcatkin willow in the skin care item
The quality percentage total amount of extract and spikemoss extract is 8.7~10.5%.Present inventor has found through test of many times, when
Sealwort stem cell extract in skin care item, echinacea pallida extract, silk-extract, bigcatkin willow extract and spikemoss extract
When quality percentage total amount is 8.7~10.5%, the anti-aging effects of skin care item are more preferably.
Preferably, the sealwort stem cell extract is prepared by the following：
S1, sealwort new life root or/and branch sterilization, after removing xylem and marrow, it is inoculated in inducing culture, induction training
Support and obtain cambial cell；
S2, the cambial cell access proliferated culture medium, shaking table culture obtains unicellular through squamous subculture；
S3, by it is described it is unicellular be inoculated in proliferated culture medium through expand cultivate, obtain sealwort stem cell；
S4, gained sealwort stem cell obtain described sealwort stem cell through the micro-nano method broken wall of low temperature, then by water extraction
Extract.
Wherein, the micro-nano method broken wall of low temperature refers to make its freezing in a low temperature of sealwort stem cell is placed on into -170 DEG C, then
Melt at normal temperatures, so repeatedly so that the cell membrane of sealwort stem cell crushes；This method can by the vital activity of stem cell with
Power of regeneration is assigned in skin care item, is absorbed via skin, can be remarkably promoted nutrition and be penetrated into and absorb, can effectively lift skin actively
Property, improve skin quality from cell aspect, can effective delaying skin aging.
Preferably, the echinacea pallida extract is prepared by the following：Echinacea chrysanthemum is through high speed bead mill method
After broken wall, echinacea pallida extract is obtained using water extraction；
The silk-extract is prepared by the following：Silk is taken to be obtained after the processing of high speed bead mill method using alcohol extracting method
To silk-extract；
The bigcatkin willow extract is prepared by the following：Yang Ye fetch water after the micro-nano method broken wall of low temperature, using water extraction
Method obtains bigcatkin willow leaf extract；
The spikemoss extract is prepared by the following：Selaginella tamariscina leaf is taken after the micro-nano method broken wall of low temperature, using water extraction
Method obtains spikemoss extract.Wherein, the preferred methanol of alcohol extracting method or ethanol are extracted.
Relative to prior art, beneficial effects of the present invention are：
Skin care item in the present invention include sealwort stem cell extract, echinacea pallida extract, silk-extract, bigcatkin willow
Extract and spikemoss extract, the nutriment in these extracts combine can effective delaying skin aging, simultaneously
Suppress bacterial reproduction, also, be free of preservative, it is useful and harmless to skin.
Embodiment
To better illustrate the object, technical solutions and advantages of the present invention, below in conjunction with specific embodiment to the present invention
It is described further.
Embodiment 1
A kind of embodiment of the anti-apolexis composition of the present invention, include the composition of following parts by weight：Sealwort stem cell is extracted
0.04 part of thing, 7.5 parts of echinacea pallida extract, 0.8 part of silk-extract, 9.2 parts of bigcatkin willow extract and spikemoss extract
0.77 part.
A kind of embodiment of the anti-aging skin care product of the present invention, includes above-mentioned anti-apolexis composition, Trivent OCG 37
Part, 20 parts of glycerine, 42 parts of Arabic gum and 88 parts of water, wherein, sealwort stem cell extract, Echinacea extraction in skin care item
Thing, silk-extract, the quality percentage total amount of bigcatkin willow extract and spikemoss extract are 10.5%.
The preparation method of above-mentioned anti-aging skin care product comprises the following steps：
(1) sealwort stem cell extract is prepared by the following：
S1, the sterilization of sealwort new life root, after removing xylem and marrow, inducing culture is inoculated in, Fiber differentiation obtains shape
Into confluent monolayer cells；
S2, cambial cell access proliferated culture medium, shaking table culture obtains unicellular through squamous subculture；
S3, by it is unicellular be inoculated in proliferated culture medium through expand cultivate, obtain sealwort stem cell；
S4, gained sealwort stem cell obtain described sealwort stem cell through the micro-nano method broken wall of low temperature, then by water extraction
Extract；
(2) echinacea pallida extract is prepared by the following：Echinacea chrysanthemum is adopted after high speed bead mill method broken wall
Echinacea pallida extract is obtained with water extraction；
Silk-extract is prepared by the following：Silk is taken to be obtained after the processing of high speed bead mill method using methanol extraction
To silk-extract；
Bigcatkin willow extract is prepared by the following：Yang Ye fetch water after the micro-nano method broken wall of low temperature, is obtained using water extraction
To bigcatkin willow leaf extract；
Spikemoss extract is prepared by the following：Selaginella tamariscina leaf is taken to be obtained after the micro-nano method broken wall of low temperature using water extraction
To spikemoss extract；
(3) by echinacea pallida extract, silk-extract, bigcatkin willow extract and spikemoss extract and Trivent OCG
88 parts of 37 parts, 20 parts of glycerine, 42 parts of Arabic gum and water mix, and after 36.3 degrees Celsius stir, are filtered to remove superfluous water
Point, lotion is obtained, then addition sealwort stem cell extract, which is uniformly dispersed, produces a kind of anti-aging skin cream.
Embodiment 2
A kind of embodiment of the anti-apolexis composition of the present invention, include the composition of following parts by weight：Sealwort stem cell is extracted
0.17 part of thing, 5.2 parts of echinacea pallida extract, 2.5 parts of silk-extract, 5.4 parts of bigcatkin willow extract and spikemoss extract 2.6
Part.
A kind of embodiment of the anti-aging skin care product of the present invention, includes above-mentioned anti-apolexis composition, Trivent OCG 32
Part, 15 parts of glycerine, 36 parts of Arabic gum and 79 parts of water, wherein, sealwort stem cell extract, Echinacea extraction in skin care item
Thing, silk-extract, the quality percentage total amount of bigcatkin willow extract and spikemoss extract are 9.3%.
The preparation method of above-mentioned anti-aging skin care product comprises the following steps：
(1) sealwort stem cell extract is prepared by the following：
(mass ratio of root and branch is 1 for S1, sealwort new life root and branch:2) sterilize, after removal xylem and marrow,
Inducing culture is inoculated in, Fiber differentiation obtains cambial cell；
S2, cambial cell access proliferated culture medium, shaking table culture obtains unicellular through squamous subculture；
S3, by it is unicellular be inoculated in proliferated culture medium through expand cultivate, obtain sealwort stem cell；
S4, gained sealwort stem cell obtain described sealwort stem cell through the micro-nano method broken wall of low temperature, then by water extraction
Extract；
(2) echinacea pallida extract is prepared by the following：Echinacea chrysanthemum is adopted after high speed bead mill method broken wall
Echinacea pallida extract is obtained with water extraction；
Silk-extract is prepared by the following：Silk is taken to be obtained after the processing of high speed bead mill method using ethanol extraction method
To silk-extract；
Bigcatkin willow extract is prepared by the following：Yang Ye fetch water after the micro-nano method broken wall of low temperature, is obtained using water extraction
To bigcatkin willow leaf extract；
Spikemoss extract is prepared by the following：Selaginella tamariscina leaf is taken to be obtained after the micro-nano method broken wall of low temperature using water extraction
To spikemoss extract；
(3) by echinacea pallida extract, silk-extract, bigcatkin willow extract and spikemoss extract and Trivent OCG
79 parts of 32 parts, 15 parts of glycerine, 36 parts of Arabic gum and water mix, and after 36.9 degrees Celsius stir, are filtered to remove superfluous water
Point, lotion is obtained, then addition sealwort stem cell extract, which is uniformly dispersed, produces a kind of anti-aging skin cream.
Embodiment 3
A kind of embodiment of the anti-apolexis composition of the present invention, include the composition of following parts by weight：Sealwort stem cell is extracted
0.35 part of thing, 3 parts of echinacea pallida extract, 4.1 parts of silk-extract, 7.9 parts of bigcatkin willow extract and spikemoss extract 5.14
Part.
A kind of embodiment of the anti-aging skin care product of the present invention, includes above-mentioned anti-apolexis composition, Trivent OCG 45
Part, 23 parts of glycerine, 51 parts of Arabic gum and 91 parts of water, wherein, sealwort stem cell extract, Echinacea extraction in skin care item
Thing, silk-extract, the quality percentage total amount of bigcatkin willow extract and spikemoss extract are 9.1%.
The preparation method of above-mentioned anti-aging skin care product comprises the following steps：
(1) sealwort stem cell extract is prepared by the following：
S1, the sterilization of sealwort new life root, after removing xylem and marrow, inducing culture is inoculated in, Fiber differentiation obtains shape
Into confluent monolayer cells；
S2, cambial cell access proliferated culture medium, shaking table culture obtains unicellular through squamous subculture；
S3, by it is unicellular be inoculated in proliferated culture medium through expand cultivate, obtain sealwort stem cell；
S4, gained sealwort stem cell obtain described sealwort stem cell through the micro-nano method broken wall of low temperature, then by water extraction
Extract；
(2) echinacea pallida extract is prepared by the following：Echinacea chrysanthemum is adopted after high speed bead mill method broken wall
Echinacea pallida extract is obtained with water extraction；
Silk-extract is prepared by the following：Silk is taken to be obtained after the processing of high speed bead mill method using ethanol extraction method
To silk-extract；
Bigcatkin willow extract is prepared by the following：Yang Ye fetch water after the micro-nano method broken wall of low temperature, is obtained using water extraction
To bigcatkin willow leaf extract；
Spikemoss extract is prepared by the following：Selaginella tamariscina leaf is taken to be obtained after the micro-nano method broken wall of low temperature using water extraction
To spikemoss extract；
(3) by echinacea pallida extract, silk-extract, bigcatkin willow extract and spikemoss extract and Trivent OCG
91 parts of 45 parts, 23 parts of glycerine, 51 parts of Arabic gum and water mix, and after 37 degrees Celsius stir, are filtered to remove excessive moisture,
Lotion is obtained, then addition sealwort stem cell extract, which is uniformly dispersed, produces a kind of anti-aging skin cream.
Embodiment 4
A kind of embodiment of the anti-apolexis composition of the present invention, include the composition of following parts by weight：Sealwort stem cell is extracted
1.9 parts of thing, 1.4 parts of echinacea pallida extract, 6.7 parts of silk-extract, 3.8 parts of bigcatkin willow extract and spikemoss extract 6.8
Part.
A kind of embodiment of the anti-aging skin care product of the present invention, includes above-mentioned anti-apolexis composition, Trivent OCG 42
Part, 23 parts of glycerine, 49 parts of Arabic gum and 86 parts of water, wherein, sealwort stem cell extract, Echinacea extraction in skin care item
Thing, silk-extract, the quality percentage total amount of bigcatkin willow extract and spikemoss extract are 8.7%.
The preparation method of above-mentioned anti-aging skin care product comprises the following steps：
(1) sealwort stem cell extract is prepared by the following：
S1, the sterilization of sealwort new life branch, after removing xylem and marrow, inducing culture is inoculated in, Fiber differentiation obtains shape
Into confluent monolayer cells；
S2, cambial cell access proliferated culture medium, shaking table culture obtains unicellular through squamous subculture；
S3, by it is unicellular be inoculated in proliferated culture medium through expand cultivate, obtain sealwort stem cell；
S4, gained sealwort stem cell obtain described sealwort stem cell through the micro-nano method broken wall of low temperature, then by water extraction
Extract；
(2) echinacea pallida extract is prepared by the following：Echinacea chrysanthemum is adopted after high speed bead mill method broken wall
Echinacea pallida extract is obtained with water extraction；
Silk-extract is prepared by the following：Silk is taken to be obtained after the processing of high speed bead mill method using methanol extraction
To silk-extract；
Bigcatkin willow extract is prepared by the following：Yang Ye fetch water after the micro-nano method broken wall of low temperature, is obtained using water extraction
To bigcatkin willow leaf extract；
Spikemoss extract is prepared by the following：Selaginella tamariscina leaf is taken to be obtained after the micro-nano method broken wall of low temperature using water extraction
To spikemoss extract；
(3) by echinacea pallida extract, silk-extract, bigcatkin willow extract and spikemoss extract and Trivent OCG
86 parts of 42 parts, 23 parts of glycerine, 49 parts of Arabic gum and water mix, and after 36.7 degrees Celsius stir, are filtered to remove superfluous water
Point, lotion is obtained, then addition sealwort stem cell extract, which is uniformly dispersed, produces a kind of anti-aging skin cream.
Embodiment 5
A kind of embodiment of the anti-apolexis composition of the present invention, include the composition of following parts by weight：Sealwort stem cell is extracted
3.7 parts of thing, 0.6 part of echinacea pallida extract, 8.3 parts of silk-extract, 1.1 parts of bigcatkin willow extract and spikemoss extract 8.9
Part.
A kind of embodiment of the anti-aging skin care product of the present invention, includes above-mentioned anti-apolexis composition, Trivent OCG 42
Part, 20 parts of glycerine, 51 parts of Arabic gum and 78 parts of water, wherein, sealwort stem cell extract, Echinacea extraction in skin care item
Thing, silk-extract, the quality percentage total amount of bigcatkin willow extract and spikemoss extract are 11.5%.
The preparation method of above-mentioned anti-aging skin care product comprises the following steps：
(1) sealwort stem cell extract is prepared by the following：
S1, the sterilization of sealwort new life branch, after removing xylem and marrow, inducing culture is inoculated in, Fiber differentiation obtains shape
Into confluent monolayer cells；
S2, cambial cell access proliferated culture medium, shaking table culture obtains unicellular through squamous subculture；
S3, by it is unicellular be inoculated in proliferated culture medium through expand cultivate, obtain sealwort stem cell；
S4, gained sealwort stem cell obtain described sealwort stem cell through the micro-nano method broken wall of low temperature, then by water extraction
Extract；
(2) echinacea pallida extract is prepared by the following：Echinacea chrysanthemum is adopted after high speed bead mill method broken wall
Echinacea pallida extract is obtained with water extraction；
Silk-extract is prepared by the following：Silk is taken to be obtained after the processing of high speed bead mill method using ethanol extraction method
To silk-extract；
Bigcatkin willow extract is prepared by the following：Yang Ye fetch water after the micro-nano method broken wall of low temperature, is obtained using water extraction
To bigcatkin willow leaf extract；
Spikemoss extract is prepared by the following：Selaginella tamariscina leaf is taken to be obtained after the micro-nano method broken wall of low temperature using water extraction
To spikemoss extract；
(3) by echinacea pallida extract, silk-extract, bigcatkin willow extract and spikemoss extract and Trivent OCG
78 parts of 42 parts, 20 parts of glycerine, 51 parts of Arabic gum and water mix, and after 37.2 degrees Celsius stir, are filtered to remove superfluous water
Point, lotion is obtained, then addition sealwort stem cell extract, which is uniformly dispersed, produces a kind of anti-aging skin cream.
The anti-aging effects test of the skin care item of the present invention of embodiment 6
Test object：The face cream of embodiment 1~5 and the face cream of control group 1~4, wherein, the skin care of control group 1~4
Frost differs only in sealwort stem cell extract, echinacea pallida extract and silk-extract with the face cream of embodiment 3
Mass ratio, the content of other compositions and the preparation method of face cream are same as Example 3 in face cream；Control group 1~4
The mass ratio of sealwort stem cell extract, echinacea pallida extract and silk-extract is 0.09 successively in face cream:3.1：
2；0.21：3.1：3.5；0.54：3.1：4.7；0.75：3.1：5.3.
Method of testing：Nine groups of face cream altogether, every group of face cream chooses the women 18 of 33~58 years old respectively, after washing one's face,
Sooner or later each to smear face once, usage time is 90 days, using German CK companies skin elasticity tester MPA580 (using constant
Negative pressure 450mbar) the skin maximum tension amount (Uf) after using preceding and use is detected, calculate being averaged for skin elasticity increment rate
Value；
Test result：It is as shown in table 1 below；
The anti-aging effects of the face cream of the present invention of table 1
| Face cream | Skin elasticity average increment rate | Face cream | Skin elasticity average increment rate |
| Embodiment 1 | 6.1% | Control group 1 | 11.2% |
| Embodiment 2 | 8.3% | Control group 2 | 14.7% |
| Embodiment 3 | 22.3% | Control group 3 | 16.1% |
| Embodiment 4 | 7.6% | Control group 4 | 11.6% |
| Embodiment 5 | 4.2% |
Interpretation of result：Understood from upper table 1 the elastic effect of the face cream increase skin of embodiment 3 and control group 1~4 compared with
It is good；Wherein, the elastic effect of the increase skin of the face cream of embodiment 3 is most strong, shows the anti-aging of the face cream of embodiment 3
Best results.
The fungistatic effect test of the skin care item of the present invention of embodiment 7
Test object：The face cream of embodiment 1~5 and as a control group 1~4 face cream, wherein, control group 1~4
Compared with the face cream of embodiment 3, the mass ratio for differing only in sealwort stem cell extract and spikemoss extract is face cream
Difference, other compositions and preparation method are identical with the face cream of embodiment 3 in face cream；Sealwort in the face cream of control group 1~4
The mass ratio of stem cell extract and spikemoss extract is followed successively by：0.11：4.7；0.45：4.7；0.54：4.7；0.68：4.7.
Method of testing：Choose staphylococcus, streptococcus, bacterium acidi propionici and corynebacteria, and LB solid mediums (i.e. LB
Flat board), face cream is dissolved into the aqueous solution that mass percent is 2.6% with water, by staphylococcus, streptococcus, bacterium acidi propionici
With corynebacteria according to quantity 1：1：1：Added after 1 mixing in the aqueous solution of face cream and form mixed solution, bacterium in mixed solution
Concentration be 1.3 × 106It is individual, drip 5 drop mixed solutions on LB flat boards with dropper respectively, the straight of bacterial plaque is measured after 55 hours
Footpath, and calculate bacterial plaque average diameter.
Test result：It is as shown in table 2 below；
The fungistatic effect of the face cream of the present invention of table 2
| Face cream | Bacterial plaque diameter | Face cream | Bacterial plaque diameter |
| Embodiment 1 | 0.42cm | Control group 1 | 0.58cm |
| Embodiment 2 | 0.21cm | Control group 2 | 0.37cm |
| Embodiment 3 | 0cm | Control group 3 | 0.46cm |
| Embodiment 4 | 0.64cm | Control group 4 | 0.52cm |
| Embodiment 5 | 0.75cm |
Interpretation of result：As shown in upper table 2, it is known that the face cream of embodiment 1~3 and control group 1~4 is antibacterial
Effect is preferable, wherein, using the antibacterial ability of the face cream of embodiment 3 to be best.
Finally, it should be noted that the above embodiments are merely illustrative of the technical solutions of the present invention rather than the present invention is protected
The limitation of scope is protected, although being explained in detail with reference to preferred embodiment to the present invention, one of ordinary skill in the art should
Understand, technical scheme can be modified or equivalent substitution, without departing from the essence of technical solution of the present invention
And scope.
Claims (10)
1. a kind of anti-apolexis composition, it is characterised in that the composition of following parts by weight is included in the anti-apolexis composition：Sealwort
0.04~3.7 part of stem cell extract, 0.6~7.5 part of echinacea pallida extract, 0.8~8.3 part of silk-extract, bigcatkin willow
0.77~8.9 part of 1.1~9.2 parts of extract and spikemoss extract.
2. anti-apolexis composition according to claim 1, it is characterised in that the sealwort stem cell extract, pale pine
The mass ratio of fruit chrysanthemum extract and silk-extract is 0.09~0.75:3.1:2~5.3.
3. anti-apolexis composition according to claim 2, it is characterised in that the sealwort stem cell extract, pale pine
The mass ratio of fruit chrysanthemum extract and silk-extract is 0.35:3:4.1.
4. anti-apolexis composition according to claim 1, it is characterised in that the sealwort stem cell extract and Selaginella tamariscina carry
The mass ratio for taking thing is 0.11~0.68:4.7.
5. anti-apolexis composition according to claim 4, it is characterised in that the sealwort stem cell extract and Selaginella tamariscina carry
The mass ratio for taking thing is 0.32:4.7.
6. anti-apolexis composition according to claim 1, it is characterised in that comprising following heavy in the anti-apolexis composition
Measure the composition of part：0.35 part of sealwort stem cell extract, 3 parts of echinacea pallida extract, 4.1 parts of silk-extract, bigcatkin willow carry
Take 5.14 parts of 7.9 parts of thing and spikemoss extract.
7. a kind of anti-aging skin care product, it is characterised in that the skin care item include the combination described in any one of claim 1~6
Thing.
8. anti-aging skin care product according to claim 7, it is characterised in that sealwort stem cell is extracted in the skin care item
Thing, echinacea pallida extract, silk-extract, the quality percentage total amount of bigcatkin willow extract and spikemoss extract for 8.7~
10.5%.
9. anti-aging skin care product according to claim 7, it is characterised in that the sealwort stem cell extract passes through following
Method obtains：
S1, sealwort new life root or/and branch sterilization, after removing xylem and marrow, inducing culture is inoculated in, Fiber differentiation obtains
Obtain cambial cell；
S2, the cambial cell access proliferated culture medium, shaking table culture obtains unicellular through squamous subculture；
S3, by it is described it is unicellular be inoculated in proliferated culture medium through expand cultivate, obtain sealwort stem cell；
S4, gained sealwort stem cell obtain described sealwort stem cell extraction through the micro-nano method broken wall of low temperature, then by water extraction
Thing.
10. anti-aging skin care product according to claim 7, it is characterised in that the echinacea pallida extract by with
Lower method obtains：Echinacea chrysanthemum obtains echinacea pallida extract after high speed bead mill method broken wall using water extraction；
The silk-extract is prepared by the following：Silk is taken to obtain silkworm using alcohol extracting method after the processing of high speed bead mill method
Silk extract；
The bigcatkin willow extract is prepared by the following：Yang Ye fetch water after the micro-nano method broken wall of low temperature, is obtained using water extraction
To bigcatkin willow leaf extract；
The spikemoss extract is prepared by the following：Selaginella tamariscina leaf is taken to be obtained after the micro-nano method broken wall of low temperature using water extraction
To spikemoss extract.
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| CN201711312847.3A CN107823117A (en) | 2017-12-08 | 2017-12-08 | A kind of anti-aging skin care product containing sealwort stem cell extract |
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| CN201711312847.3A CN107823117A (en) | 2017-12-08 | 2017-12-08 | A kind of anti-aging skin care product containing sealwort stem cell extract |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110623890A (en) * | 2018-06-21 | 2019-12-31 | 伽蓝（集团）股份有限公司 | Application of polygonatum kingianum extract |
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| CN104920227A (en) * | 2015-07-15 | 2015-09-23 | 广东融和生态农业有限公司 | Stem cell tissue culture seedling raising technique |
| CN105748343A (en) * | 2016-02-25 | 2016-07-13 | 广州市科玮生物技术有限公司 | Natural plant extract composition and application thereof in cosmetics |
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2017
- 2017-12-08 CN CN201711312847.3A patent/CN107823117A/en not_active Withdrawn
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN104920227A (en) * | 2015-07-15 | 2015-09-23 | 广东融和生态农业有限公司 | Stem cell tissue culture seedling raising technique |
| CN105748343A (en) * | 2016-02-25 | 2016-07-13 | 广州市科玮生物技术有限公司 | Natural plant extract composition and application thereof in cosmetics |
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| 王建新等: "《化妆品植物原料大全》", 30 June 2012, 中国纺织出版社 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN110623890A (en) * | 2018-06-21 | 2019-12-31 | 伽蓝（集团）股份有限公司 | Application of polygonatum kingianum extract |
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