CN107812213A - A kind of wild silkworm ovum disinfection treatment method - Google Patents
A kind of wild silkworm ovum disinfection treatment method Download PDFInfo
- Publication number
- CN107812213A CN107812213A CN201711211589.XA CN201711211589A CN107812213A CN 107812213 A CN107812213 A CN 107812213A CN 201711211589 A CN201711211589 A CN 201711211589A CN 107812213 A CN107812213 A CN 107812213A
- Authority
- CN
- China
- Prior art keywords
- silkworm
- silkworm seed
- treatment method
- disinfection treatment
- clear water
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2/00—Methods or apparatus for disinfecting or sterilising materials or objects other than foodstuffs or contact lenses; Accessories therefor
- A61L2/0005—Methods or apparatus for disinfecting or sterilising materials or objects other than foodstuffs or contact lenses; Accessories therefor for pharmaceuticals, biologicals or living parts
- A61L2/0082—Methods or apparatus for disinfecting or sterilising materials or objects other than foodstuffs or contact lenses; Accessories therefor for pharmaceuticals, biologicals or living parts using chemical substances
- A61L2/0088—Liquid substances
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; CARE OF BIRDS, FISHES, INSECTS; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K67/00—Rearing or breeding animals, not otherwise provided for; New breeds of animals
- A01K67/033—Rearing or breeding invertebrates; New breeds of invertebrates
- A01K67/04—Silkworms
Abstract
The invention provides a kind of wild silkworm ovum disinfection treatment method, comprise the following steps：1) sodium hydroxide solution dipping cleaning silkworm seed, removes the debris on silkworm seed surface, and clear water elution obtains pre-sterilization silkworm seed；2) ClO 2 solution impregnation steps 1 are used) in pre-sterilization silkworm seed obtain sterilization silkworm seed；The pH value of the ClO 2 solution is 9~10；3) the sterilization silkworm seed clear water for obtaining step 2) rinses, and divides, and dries to obtain and waits to be incubated spawning.The scheme carries out disinfection processing, and step is few, and the operating time is short；The concentration range of ClO 2 solution is wide in range, simple to operate, now with the current；Methods described can efficiently kill the pathogenic microorganisms such as bacterium entrained by wild silkworm ovum surface, virus, micro- spore；It is harmless, it is environmentally friendly；There is no poisoning to silkworm seed, security is good, and incubation rate is high.
Description
Technical field
The present invention relates to wild silkworm ovum disinfection technology field, more particularly to a kind of wild silkworm ovum disinfection treatment method.
Background technology
After wild silkworm (such as tussah, giant silkworm, amber silkworm, improving eyesight grown silkworm) spawning, ovum face is not only stained with dust, palea, moth
Urine etc., and also substantial amounts of pathogenic microorganism.During egg-incubation, the bite-actuated chorion of larva drills out, then typically all
It can reverse end for end to sting and eat the chorion of oneself, pathogenic microorganism can then be eaten down in the lump, cause silkworm body introduced disease, therefore wild silkworm egg hatching
Before must carry out the disinfection of egg surface.
The silkworm seed disinfection of egg surface is a kind of technology of comparative maturity, mainly has the disinfectants such as formaldehyde, hydrochloric acid, bleaching powder to be configured
Decoction.Common disinfection concentration and disinfecting time is as shown in table 1.
Table 1：Common silkworm seed disinfection of egg surface medicament kind, concentration and disinfecting time
| Sequence number | Medicament | Liquor strength (wt%) | Disinfecting time (min) |
| 1 | Formaldehyde | 3 | 30 |
| 2 | Bleaching powder | 1 | 5 |
| 3 | Hydrochloric acid | 10 | 10 |
| 4 | Sulfuric acid | 5 | 10 |
| 5 | Formaldehyde+hydrochloric acid | 3,3 | 30 |
It is short with 1% bleaching fluid disinfecting time in common disinfectant, it is good to pathogenic microorganism killing effect.But
Now to be surveyed when being that bleaching powder is a kind of unstable medicament, be easier to fail, therefore preparing 1% bleaching fluid with existing, measure
It is comparatively laborious with compound method.The concentration and time sterilized with bleaching fluid must be controlled accurately, and disinfection concentration is too high and sterilizes
Overlong time, easily produce poisoning.The problems such as in view of cost medication effect, the disinfection of egg surface is done with simple hydrochloric acid or sulfuric acid
It is more rare.
The most frequently used disinfectant of the disinfection of egg surface is the mixed liquor of formaldehyde and formaldehyde+hydrochloric acid.Usually with 36% formaldehyde 1
Part is watered 11 parts；Or by 36% formaldehyde：35% hydrochloric acid：Water=1:1:10 ratio mixes.This disinfection of egg surface security
Height, largely used on silkworm and tussah production.There is also some problems, formaldehyde simultaneously to be commonly called as formalin, is a kind of industry
With preservative, there is strong carcinogenic and tumor promotion.Influence of the formaldehyde to health is mainly manifested in allotriosmia, thorn
Sharp, allergy, abnormal pulmonary function, dysfunction of liver and immunologic dysfunction.Long Term Contact formaldehyde, easily cause people's slow poisoning,
Trigger the diseases such as respiratory system, nervous system, reproductive system.Require that operating personnel do not stop when being sterilized in production to substantial amounts of silkworm seed
Stir silkworm seed, 30min disinfecting time, make one the volatile formaldehyde gas for having to contact high concentration, be detrimental to health.
Formaldehyde carries out ovum face to silkworm seed, it is desirable to which purpose concentration 3%, dip time are no less than 30min, to diseases such as virus, bacterium, micro- spores
The killing effect of pathogenic microorganism is just obvious, and excessive concentration easily produces poisoning, and the too low killing effect of concentration is bad, or needs to impregnate
Time it is longer.Such as Liu Chaoliang (1996) [influence research [J] peaces that Liu Chaoliang, Lu Zhen inscription disinfection of egg surfaces liquid are hatched to giant silkworm
Emblem agricultural sciences, 1996, (01):60-62] to being found during the giant silkworm disinfection of egg surface, the disinfection of egg surface, different drug concentrations, no
Same disinfecting time, the different reagent removal time, has large effect to the incubation rate of giant silkworm.Moreover the disinfection of egg surface requires medicine
Liquid is used while allocating, and used formaldehyde, hydrochloric acid can only abandon, and certain pollution can be caused to environment.Therefore, safe efficient, environment
Friendly wild silkworm disinfection of egg surface processing method has important realistic meaning.
The content of the invention
In view of this, it is an object of the invention to provide a kind of safe efficient, environment-friendly wild silkworm ovum side of disinfecting
Method.
In order to realize foregoing invention purpose, the present invention provides following technical scheme：A kind of wild silkworm ovum disinfection treatment method, bag
Include following steps：1) sodium hydroxide solution dipping cleaning silkworm seed, removes the debris on silkworm seed surface, and clear water elution obtains pre-sterilization silkworm
Ovum；2) ClO 2 solution impregnation steps 1 are used) in pre-sterilization silkworm seed obtain sterilization silkworm seed；The time of the dipping be 10~
20min；Dipping temperature is 22~26 DEG C；The concentration of the chlorine dioxide is 100-500mg/L；3) sterilization for obtaining step 2)
Silkworm seed clear water rinses, and divides, and dries to obtain and waits to be incubated spawning.
Preferably, the wild silkworm ovum is amber silkworm seed.
Preferably, the mass concentration of the sodium hydroxide solution is 0.4~0.6%.
Preferably, the time of the sodium hydroxide solution dipping cleaning is 30~90s.
Preferably, the number of the clear water elution is 1~3 time, and the total time of the clear water elution is 20~30min.
Preferably, the ClO 2 solution is to be mixed with to form with water by chlorine dioxide effervescent tablet.
Preferably, the mass fraction of chlorine dioxide is 11~13% in the chlorine dioxide effervescent tablet.
Preferably, the ratio of weight and number of the chlorine dioxide effervescent tablet and water is 1：(240~1200).
Preferably, the time of the clear water rinsing described in step 3) is 5~10min.
Preferably, sterilizing to divide after the rinsing of silkworm seed clear water described in step 3) and dry, the thickness divided is 0.5~
1cm。
Beneficial effects of the present invention：Wild silkworm ovum disinfection treatment method of the present invention, using ClO 2 solution to open country
Silkworm seed carries out disinfection processing, and the operating time is short；The concentration range of ClO 2 solution is wide in range, simple to operate, now with the current；It is described
Method can efficiently kill the pathogenic microorganisms such as bacterium entrained by wild silkworm ovum surface, virus, micro- spore；It is harmless, it is right
It is environment-friendly；There is no poisoning to silkworm seed, security is good, and incubation rate is up to 99.4%.
Embodiment
The invention provides a kind of wild silkworm ovum disinfection treatment method, comprise the following steps：1) sodium hydroxide solution dipping is clear
Clean silkworm seed, the debris on artificial removal's silkworm seed surface, clear water elution obtain pre-sterilization silkworm seed；2) ClO 2 solution impregnation steps are used
1) the pre-sterilization silkworm seed in obtains sterilization silkworm seed；The time of the dipping is 10~20min；Dipping temperature is 22~26 DEG C；Institute
The concentration for stating chlorine dioxide is 100~500mg/L；3) the sterilization silkworm seed clear water for obtaining step 2) rinses, and divides, dries acquisition
Wait to be incubated spawning.
In the present invention, the wild silkworm ovum disinfection treatment method is applied to the open countries such as tussah, giant silkworm, amber silkworm, improving eyesight grown silkworm
Silkworm seed sterilizes, preferably amber silkworm seed.The amber silkworm Antheraeaassama is a kind of rare tussah category spun silk elder brother
Worm, amber silk are in natural golden yellow, and noble magnificent very worthy, now only India can produce on a small quantity.The wild silkworm ovum is protected
The temperature of dis environment is preferably 24~26 DEG C, more preferably 25 DEG C；Humidity is preferably 75~85%, more preferably 78
~82%.The time of the silkworm seed sterilization is 5~8 days after spawning in the present invention, 2~3 days before hatching.
In the present invention, cleaning silkworm seed is impregnated with sodium hydroxide solution first, while removes the debris on silkworm seed surface.At this
In invention, the mass concentration of the sodium hydroxide solution is preferably 0.4~0.6%, more preferably 0.5%；The dipping
The time of cleaning is preferably 30~90s, more preferably 40~70s, most preferably 60s；The sodium hydroxide solution leaching
Stain cleaning act as cleaning silkworm seed, the removal deadlocked thing in silkworm seed surface.In the present invention, it is preferred to silkworm seed is mounted in gauze sack
It is interior, sodium hydroxide solution dipping is put in, the present invention removes silkworm seed surface during sodium hydroxide solution impregnates cleaning silkworm seed
Debris；The debris includes palea, moth urine, dust, the deadlocked thing of silkworm seed etc.；Silkworm seed surface irregularities are specifically removed in the present invention
Method for by hand wash by rubbing with the hands.The present invention preferably carries out filtrate, will be stained with silkworm seed surface after sodium hydroxide solution impregnates silkworm seed
Sodium hydroxide solution filter.
The present invention is eluted with clear water after silkworm seed surface irregularities are removed and obtains pre-sterilization silkworm seed.In the present invention, it is described clear
The number of the clear water elution of water elution is preferably 1~3 time, more preferably 2 times；The total time of the clear water elution is preferable
For 20~30min, more preferably 22~28min, most preferably 25min.The present invention is filtered after clear water elution
Water, filter the water on silkworm seed surface.
The present invention impregnates pre-sterilization silkworm seed after pre-sterilization silkworm seed is obtained, with ClO 2 solution and obtains sterilization silkworm seed.Institute
The time for stating dipping is 10~20min, preferably 12~18min；The dipping temperature be 22~26 DEG C, preferably 23~
25℃.The concentration of the ClO 2 solution is 100~500mg/L in the present invention；Preferably 200~400mg/L；It is described
The pH value of ClO 2 solution is preferably 9~10.
ClO 2 solution described in the present invention is preferably mixed with water by chlorine dioxide effervescent tablet and formed.
The mass fraction of chlorine dioxide is preferably 11~13% in the chlorine dioxide effervescent tablet in the present invention, more preferably
12%.In the present invention, the ratio of weight and number of the chlorine dioxide effervescent tablet and water is preferably 1：(240~1200), it is more excellent
Choosing for 1：(400~1000).In the present invention, the source of the chlorine dioxide effervescent tablet is preferably commercial goods；It is described
Commercially available chlorine dioxide effervescent tablet is stabilization formulations, dissolves after entering water, does not almost remain at once, using more convenient；The dioxy
Change the preferable kept dry of chlorine effervescent tablet.
In the present invention, the ClO 2 solution is adjusted using alkaline matter after chlorine dioxide effervescent tablet mixes with water
PH value is 9~10；The alkaline matter is preferably sodium hydroxide, potassium hydroxide or sodium carbonate.Chlorine dioxide in the present invention
Solution is advantageous to kill Viruses With Inclusion Body particle rapidly in the case where pH value is 9.0~10 alkaline environment.
The present invention rinses sterilization silkworm seed after the sterilization silkworm seed of acquisition, with clear water, divides, and dries to obtain and waits to be incubated spawning.
The time of the clear water rinsing is preferably 5~10min, more preferably 6~9min in the present invention.In the present invention, institute
State the thickness divided and be preferably 0.5~1cm, more preferably 0.6~0.8cm.The heretofore described temperature dried is preferred
For 24~26 DEG C.
The present invention will sterilize silkworm seed and be placed in 24~26 DEG C, under 75~85% humiture environment after obtaining and sterilizing silkworm seed
Protection, wait egg-incubation.
Wild silkworm ovum disinfection treatment method provided by the invention is described in detail with reference to embodiment, but can not
They are interpreted as limiting the scope of the present invention.
Embodiment 1
(1) after the spawning of amber silkworm, microscopy is nontoxic, the child care 7 days at 25 DEG C of temperature, the environment of humidity 80%.(2) by silkworm seed
In gauze sack, 0.5% sodium hydroxide 60s is immersed in, puts into clear water and rinses 1 time after filtrate, drainage.(3) with containing
The chlorine dioxide effervescent tablet 3g (about 2) for 12% is measured, 1200ml is watered, obtains 300mg/L chlorine dioxide disinfection liquid, is used
The pH value of thimerosal is transferred to 9.5 by 0.5% sodium hydroxide solution.(4) silkworm seed is immersed in chlorine dioxide disinfection liquid
10min, 24 DEG C of liquid temperature, cleaned 1 time with water, drainage dries dry.(5) collect silkworm seed and be placed on 25 DEG C of temperature, the environment of humidity 80%
Lower child care, wait hatching.
The incubation rate contrast of silkworm seed is shown in Table 2, and the incubation rate of the wherein silkworm seed after ClO 2 solution sterilization is 99.0% (system
Ovum of not inseminating is removed in meter).Control is the formaldehyde with 3%, impregnates silkworm seed 30min, after cleaning is dried, incubation rate 99.4%,
It is suitable with disinfection agent of chlorine dioxide.It can be seen that the disinfection of egg surface that chlorine dioxide is used for amber silkworm seed is safely without poisoning.
The incubation rate result of the silkworm seed of table 2
Embodiment 2
(1) by 500g nontoxic amber silkworm seed dress gauze sack, 0.5% sodium hydroxide 40s, filtrate 20s are immersed in,
Put into clear water and rinse 2 times again, drainage.(2) with the chlorine dioxide effervescent tablet 6g (about 4) that content is 12%, 3600ml is watered,
200mg/L chlorine dioxide disinfection liquid is obtained, the pH value of thimerosal is transferred to 9.5 with 1.0% sodium hydroxide solution.(4) will
Silkworm seed is immersed in 15min in thimerosal, 24 DEG C of liquid temperature, is rinsed 2 times with clear water, drainage, thin airing is done.(5) silkworm seed is regathered to put
The child care at 25 DEG C of temperature, the environment of humidity 80% is put, waits hatching.Take 300 treated silkworm seed investigation incubation rates：Silkworm seed
Average percentage hatch rate is 92.5% (not hatching most for ovum of not inseminating in silkworm seed), and the incubation rate for removing ovum of not inseminating is
98.7%, it is considered as normal incubation.
Embodiment 3
(1) by 400g nontoxic amber silkworm seed dress gauze sack, 0.5% sodium hydroxide 40s, filtrate 20s are immersed in,
Put into clear water and rinse 2 times again, drainage.(2) with the chlorine dioxide effervescent tablet 4.5g (about 3) that content is 12%, it is watered
1200ml, 450mg/L chlorine dioxide disinfection liquid is obtained, be transferred to the pH value of thimerosal with 1.0% sodium hydroxide solution
9.8.(4) silkworm seed is immersed in 15min in thimerosal, 26 DEG C of liquid temperature, rinsed 2 times with clear water, drainage, thin airing is done.(5) receive again
Collection silkworm seed is placed on 25 DEG C of temperature, child care under the environment of humidity 80%, waits hatching.Take 260 treated silkworm seed investigation hatchings
Rate：The average percentage hatch rate of silkworm seed is 92.5% (not hatching most for ovum of not inseminating in silkworm seed), removes incubating for ovum of not inseminating
Rate is 98.9%, is considered as normal incubation.
Embodiment 4
(1) by 450g nontoxic amber silkworm seed dress gauze sack, 0.5% sodium hydroxide 50s, filtrate 20s are immersed in,
Put into clear water and rinse 3 times again, drainage.(2) with the chlorine dioxide effervescent tablet 6g (about 4) that content is 12%, 3600ml is watered,
300mg/L chlorine dioxide disinfection liquid is obtained, the pH value of thimerosal is transferred to 9.8 with 1.0% sodium hydroxide solution.(4) will
Silkworm seed is immersed in 15min in thimerosal, 26 DEG C of liquid temperature, is rinsed 2 times with clear water, drainage, thin airing is done.(5) silkworm seed is regathered to put
The child care at 25 DEG C of temperature, the environment of humidity 80% is put, waits hatching.Take 260 treated silkworm seed investigation incubation rates：Silkworm seed
Average percentage hatch rate is 92.5% (not hatching most for ovum of not inseminating in silkworm seed), and the incubation rate for removing ovum of not inseminating is
98.9%, it is considered as normal incubation.
From above-described embodiment, wild silkworm ovum disinfection treatment method of the present invention, using ClO 2 solution to open country
Silkworm seed carries out disinfection processing, and the operating time is short；The concentration range of ClO 2 solution is wide in range, simple to operate, now with the current；It is described
Method can efficiently kill the pathogenic microorganisms such as bacterium entrained by wild silkworm ovum surface, virus, micro- spore；It is harmless, it is right
It is environment-friendly；There is no poisoning to silkworm seed, security is good, and incubation rate is up to 99.4%.
Described above is only the preferred embodiment of the present invention, it is noted that for the ordinary skill people of the art
For member, under the premise without departing from the principles of the invention, some improvements and modifications can also be made, these improvements and modifications also should
It is considered as protection scope of the present invention.
Claims (10)
1. a kind of disinfection treatment method of wild silkworm ovum, comprises the following steps：
1) sodium hydroxide solution is impregnated into cleaning silkworm seed, removes the debris on silkworm seed surface, then clear water elution obtains pre-sterilization silkworm seed；
2) the pre-sterilization silkworm seed impregnated with ClO 2 solution in the step 1) obtains sterilization silkworm seed；The time of the dipping is
10~20min；Dipping temperature is 22~26 DEG C；The concentration of the ClO 2 solution is 100~500mg/L；The titanium dioxide
The pH value of solutions of chlorine is 9~10；
3) the sterilization silkworm seed clear water for obtaining the step 2) rinses, and dries to obtain and waits to be incubated spawning.
2. disinfection treatment method according to claim 1, it is characterised in that the wild silkworm ovum is amber silkworm seed.
3. disinfection treatment method according to claim 1, it is characterised in that the mass concentration of the sodium hydroxide solution is
0.4~0.6%.
4. the disinfection treatment method according to claim 1 or 3, it is characterised in that sodium hydroxide solution in the step 1)
The time of dipping cleaning is 30~90s.
5. disinfection treatment method according to claim 1, it is characterised in that the number of clear water elution is in the step 1)
1~3 time, the total time of the clear water elution is 20~30min.
6. disinfection treatment method according to claim 1, it is characterised in that the ClO 2 solution is by chlorine dioxide
Effervescent tablet is mixed with water and formed.
7. disinfection treatment method according to claim 6, it is characterised in that chlorine dioxide in the chlorine dioxide effervescent tablet
Mass fraction be 11~13%.
8. the disinfection treatment method according to claim 6 or 7, it is characterised in that the chlorine dioxide effervescent tablet and water
Weight ratio is 1：(240~1200).
9. disinfection treatment method according to claim 1, it is characterised in that the time of the clear water rinsing described in step 3)
For 5~10min.
10. disinfection treatment method according to claim 1, it is characterised in that the drift of silkworm seed clear water is sterilized described in step 3)
Divide and dry after washing, the thickness divided is 0.5~1cm.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201711211589.XA CN107812213B (en) | 2017-11-28 | 2017-11-28 | Disinfection treatment method for wild silkworm eggs |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201711211589.XA CN107812213B (en) | 2017-11-28 | 2017-11-28 | Disinfection treatment method for wild silkworm eggs |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN107812213A true CN107812213A (en) | 2018-03-20 |
| CN107812213B CN107812213B (en) | 2020-07-03 |
Family
ID=61610446
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201711211589.XA Active CN107812213B (en) | 2017-11-28 | 2017-11-28 | Disinfection treatment method for wild silkworm eggs |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN107812213B (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115349500A (en) * | 2022-09-21 | 2022-11-18 | 云南省农业科学院蚕桑蜜蜂研究所 | Method for feeding amber silkworm larva by living plant |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114391518B (en) * | 2021-12-29 | 2022-11-25 | 广东省农业科学院蚕业与农产品加工研究所 | Silkworm egg hatching method based on warm water immersion |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1631161A (en) * | 2004-10-30 | 2005-06-29 | 安徽省农业科学院蚕桑研究所 | Disinfecting agent used for silkworm containing stable chlorine dioxide |
| CN102696559A (en) * | 2012-07-05 | 2012-10-03 | 湖南南岭天蚕科技发展有限公司 | Hatching method of Nanling wild silkworm |
-
2017
- 2017-11-28 CN CN201711211589.XA patent/CN107812213B/en active Active
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1631161A (en) * | 2004-10-30 | 2005-06-29 | 安徽省农业科学院蚕桑研究所 | Disinfecting agent used for silkworm containing stable chlorine dioxide |
| CN102696559A (en) * | 2012-07-05 | 2012-10-03 | 湖南南岭天蚕科技发展有限公司 | Hatching method of Nanling wild silkworm |
Non-Patent Citations (1)
| Title |
|---|
| 黄扬玉，虞崇江，陆俣伽: "《3种转青卵面消毒处理对蚕种孵化率和蚁蚕生命率的影响试验》", 《广西蚕业》 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115349500A (en) * | 2022-09-21 | 2022-11-18 | 云南省农业科学院蚕桑蜜蜂研究所 | Method for feeding amber silkworm larva by living plant |
| CN115349500B (en) * | 2022-09-21 | 2023-10-20 | 云南省农业科学院蚕桑蜜蜂研究所 | Method for breeding silkworm larvae with living plants |
Also Published As
| Publication number | Publication date |
|---|---|
| CN107812213B (en) | 2020-07-03 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN104013573B (en) | Available iodine content is the liquid PVP-I of 5-12% | |
| WO2018024087A1 (en) | Air purification disinfectant and preparation method | |
| CN104082353A (en) | Pure traditional Chinese medicine disinfectant and preparation method thereof | |
| CN105255600A (en) | Plant-sourced vegetable and fruit pesticide detergent | |
| CN105767034A (en) | Disinfectant for hog house | |
| CN106879642A (en) | A kind of pig house disinfectant and preparation method thereof | |
| CN105753110A (en) | Functional water and multiple application methods thereof | |
| CN106265146A (en) | A kind of P-Cymene pasteurization towelette and preparation method thereof | |
| CN106912485A (en) | Compound double-chain quaternary ammonium salt thimerosal and its application method and application | |
| CN107812213A (en) | A kind of wild silkworm ovum disinfection treatment method | |
| CN105519588B (en) | A kind of disinfectant and its preparation method and application | |
| CN101911951B (en) | Medical disinfectant composition for rearing instrument in silkworm rearing room and application thereof | |
| CN106234388B (en) | A kind of composition pesticide of alkene containing benzo fluorine bacterium azoles and jamaicin | |
| CN102349548A (en) | Environment biological disinfectant with phages as main components and application thereof | |
| CN104188848B (en) | Cooperatively used combined bag for cleaning and disinfecting skin after dog and animal injuries | |
| CN106726941A (en) | A kind of moisture-keeping bacterium-restraining wet tissue and preparation method thereof | |
| CN105770999A (en) | Composite biological material for eliminating bacterial biofilm | |
| CN109699686A (en) | A kind of disinfectant for killing avian influenza virus | |
| CN207898697U (en) | A kind of barreled wet tissue production run equipment | |
| CN107603777A (en) | A kind of Chinese medicine environmental-protective detergent and preparation method thereof | |
| DE19781549B4 (en) | disinfecting composition | |
| CN107582737A (en) | A kind of compound chlorohexidene oral-cavity disinfectant and preparation method thereof | |
| CN106614781A (en) | Household disinfectant and preparation method thereof | |
| CN106719938A (en) | A kind of pigsty sterilization Chinese medicine antiseptic and preparation method thereof | |
| CN111096340A (en) | Disinfectant concentrated solution for pet hospitals and preparation method thereof |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |