A kind of protein peptides-slow-digestion starch particle and preparation method thereof
Technical field
The invention belongs to starch extract deep process technology field, and in particular to a kind of protein peptides-slow-digestion starch particle and its
Preparation method.
Background technology
In recent years, with the improvement of living standards, rhythm of life is accelerated, habits and customs and dietary structure change, more
Carry out the incidence of disease more and more higher of more chronic diseases such as high fat of blood, diabetes, cardiovascular and cerebrovascular disease, obesity etc., especially
The incidence of disease increase of obesity and diabetes is the most obvious.Therefore, the nutrition of food, low blood of the exploitation with healthcare function are paid attention to
Sugared index (Glycemic index, GI) value novel foodstuff, energy needed for reasonable supplement its body, keep the meals knot of science
Structure, it is the key point for preventing above-mentioned chronic disease.Slow-digestion starch (slowly digestible starch, SDS) is one
The stable source of glucose of kind, have and keep postprandial blood sugar stabilization, the slow physiological function for releasing energy and strengthening satiety, this
Not only contribute to stabilizing blood sugar level, and satiety can also be given, thus slow-digestion starch can control with pre- preventing obesity,
Prevention and treatment to diabetes are also helpful.
At present, the SDS in China preparation is still in laboratory stage, and research is still in starting developing stage.Wherein, starch
Through Overheating Treatment, chemical modification, ferment treatment and reach that the report of slow digestion effect is a lot, methods that these researchs use all change
Become the structure of starch itself and reach what is digested slowly, the problem of it is single nutrition to be present, high energy consumption, and environmental pollution is big.
The content of the invention
In view of this, it is an object of the invention to provide a kind of nutritious, low energy consumption, free of contamination protein peptides-disappear slowly
Change protein peptides-slow-digestion starch particle that the preparation method of starch granules and this method prepare.
In order to realize foregoing invention purpose, the present invention provides following technical scheme:
The invention provides a kind of preparation method of protein peptides-slow-digestion starch particle, comprise the following steps:1) by rice
Homogeneous after starch, protein peptides mix with water, obtain rice starch-albumen peptide complexes;2) it is big by what is obtained in the step 1)
Rice starch-albumen peptide complexes are spray-dried, and obtain protein peptides-slow-digestion starch particle;Protein peptides described in step 1)
Quality be rice starch quality 2.5~15%.
Preferably, the quality of protein peptides described in step 1) is the 0.25~1.5% of the quality of water.
Preferably, the protein peptides are grass carp protein peptides.
Preferably, the molecular weight of the grass carp protein peptides is 300~1000 dalton.
Preferably, the method mixed described in step 1) is magnetic agitation, the speed of the magnetic agitation for 400~
600rpm, the time of the magnetic agitation is 5~15min.
Preferably, the pressure of the homogeneous described in step 2) is 30~70MPa, and the time of the homogeneous is 3~10min.
Preferably, the material flow of the spray drying described in step 3) is 0.5~1.5g/min, and hot blast flow velocity is 220
~280L/min.
Preferably, the nozzle of affiliated spray drying is 75 μm of nozzles.
Preferably, the tower top temperature of the spray drying described in step 3) is 150-210 DEG C, column bottom temperature 80-100
℃。
Present invention also offers the protein peptides prepared by above-mentioned preparation method-slow-digestion starch particle, the albumen
The mass content of protein is 5~15% in peptide-slow-digestion starch particle, and the mass content of slow-digestion starch is 50~56%.
Beneficial effects of the present invention:The preparation method of protein peptides of the present invention-slow-digestion starch particle is formed sediment with rice
Powder is primary raw material, adds dispensing protein peptides, carries out homogenization combination spray drying and obtains protein peptides-slow-digestion starch
Grain.Raw Materials Rice starch, dispensing protein peptides and water are only related in the method for the invention, carrying out compound physical to rice starch changes
Property, it is modified compared to single one physical, protein peptides-slow-digestion starch particle that the method for the invention obtains is nutritious (except slow
Also include protein beyond digestible starch particle);Whole process mild condition, it can be related to rice starch with strong acid or highly basic
Row processing, therefore production process is pollution-free, it is environmentally friendly;The albumen that methods described obtains is recorded according to embodiments of the present invention
The mass content of protein may be up to 15% in peptide-slow-digestion starch particle, and the mass content of slow-digestion starch may be up to 56%.
Brief description of the drawings
Fig. 1 is untreated rice starch granule electron microscope;
Fig. 2 is that Typical physical is modified the rice starch granule electron microscope obtained;
Fig. 3 is protein peptides-slow-digestion starch particle electron microscope that the method for embodiment 1 obtains.
Embodiment
The invention provides the invention provides a kind of preparation method of protein peptides-slow-digestion starch particle, including it is following
Step:1) homogeneous after mixing rice starch, protein peptides with water, rice starch-albumen peptide complexes are obtained;2) by the step
1) rice starch-albumen peptide complexes obtained in are spray-dried, and obtain protein peptides-slow-digestion starch particle;Step 1)
Described in protein peptides quality be rice starch quality 2.5~15%.
In the present invention, the protein peptides are preferably grass carp protein peptides, and the molecular weight of the grass carp protein peptides is preferable
For 300~1000 dalton, the preferably refined grass carp protein peptides of the grass carp protein peptides.In specific implementation process of the present invention
Refined grass carp protein peptides are obtained after preferably being refined using commercially available molecular weight less than the protein peptides of 2000 dalton.In the present invention
Embodiment in, the refined grass carp protein peptides can use Shandong Zhong Shidouqing Bioisystech Co., Ltd commercial goods.
Can voluntarily it be prepared using process for purification well known to those skilled in the art, the process for purification of the grass carp protein peptides preferably wraps
Include following steps:Commercially available grass carp protein peptides are dissolved in the acquisition grass carp protein peptides aqueous solution in distilled water, then cut using ultrafiltration
Stay film to carry out ultrafiltration and obtain refined grass carp albumen peptide solution, purified protein peptide solution is freeze-dried obtain molecular weight be 300~
The refined grass carp protein peptides of 1000 dalton.The commercially available grass carp protein peptides are preferably dissolved in the distilled water of 15~20 times of volumes
In, more preferably it is dissolved in the distilled water of 20 times of volumes.The present invention is not particularly limited to the condition of described ultrafiltration, using this
The conventional hyperfiltration process in field.Ultrafiltration of the present invention obtains refined grass carp albumen peptide solution and freeze-drying acquisition essence is carried out to it
Grass carp protein peptides processed.The present invention is preferably standby in room storage by the refined grass carp protein peptides obtained in implementation process.
In the present invention, homogeneous after rice starch, protein peptides being mixed with water, rice starch-albumen peptide complexes are obtained.
In the present invention, mixed first by rice starch, protein peptides with water and obtain rice starch-protein peptides suspension.In the present invention,
The source of the rice starch is not particularly limited, using commercial goods.The quality of the protein peptides in the present invention
For the 2.5~15% of rice starch quality, preferably 5~12%;The quality of the protein peptides for water quality 0.25~
1.5%, preferably 0.3~1.4%, most preferably 0.6~1.0%.It is preferable first in specific implementation process of the present invention
After the protein peptides formation protein peptides aqueous solution soluble in water, then rice starch is mixed with the protein peptides aqueous solution and obtains rice
Starch-protein peptides suspension.The mixing is preferably mixed using the method for magnetic agitation in the present invention, the magnetic force
The rotating speed of stirring is preferably 400~600rpm, more preferably 500rpm;The time of the magnetic agitation is preferably 5~
15min, more preferably 8~12min.The effect of heretofore described magnetic agitation is rice starch is abundant with protein peptides
It is mixed to form suspension.
The present invention enters after rice starch-protein peptides suspension is obtained to the rice starch-protein peptides suspension obtained
Row homogeneous obtains rice starch-albumen peptide complexes.In the present invention, the homogeneous is preferably high-pressure homogeneous, the homogeneous
Equipment preferably uses high pressure homogenizer.The pressure of the homogeneous is preferably 30~70MPa in the present invention, more preferably
40~60MPa;The time of the homogeneous is preferably 3~10min, more preferably 5~8min.The homogeneous in the present invention
Step is advantageous to improve the stability of rice starch-grass carp albumen peptide complexes.
The present invention carries out spray drying to it and obtains protein peptides-slow digestion after rice starch-albumen peptide complexes are obtained
Starch granules.The spray drying preferably uses the micro-fluidic spray drying tower of laboratory level in the present invention, in the present invention
Used nozzle is preferably 0.75 μm of nozzle.The material flow of described spray drying is preferably 0.5~1.5g/min,
More preferably 0.8~1.2g/min, most preferably 1.0g/min.Heretofore described hot blast flow velocity is preferably 220~
280L/min, more preferably 240~260L/min, most preferably 250L/min.Spray drying described in the present invention
Tower top temperature be preferably 150~210 DEG C, more preferably 170~190 DEG C;The column bottom temperature is preferably 80~100
DEG C, more preferably 85~95 DEG C.
The present invention obtains protein peptides-slow-digestion starch particle after spray drying.Present invention also offers by above-mentioned preparation
The protein peptides that method prepares-slow-digestion starch particle, the quality of protein contains in the protein peptides-slow-digestion starch particle
Amount preferably 5~15%, the mass content of slow-digestion starch is preferably 50~56%.Protein peptides-the slow-digestion starch
The granularity of particle is 30~100 μm.
Preparation method and this method with reference to embodiment to protein peptides provided by the invention-slow-digestion starch particle
The protein peptides prepared-slow-digestion starch particle is described in detail, but they can not be interpreted as protecting the present invention
Protect the restriction of scope.
Embodiment 1
Grass carp protein peptides refine, and the molecular weight for weighing the sale of 50g Shandong Zhong Shidouqing Bioisystech Co., Ltd is less than
The protein peptides of 2000 dalton, are dissolved in 1000mL distilled water, using molecular weight as the super of 300 dalton and 1000 dalton
Mwco membrane is filtered, carries out ultrafiltration, after the grass carp protein purification peptide solution for obtaining 300-1000 dalton, is obtained after being freeze-dried
Refined grass carp protein peptides, it is standby to store at room temperature.
Weigh the grass carp protein peptides 5g that molecular weight is 300-1000 dalton and be dissolved in 1000mL distilled water, then add 100g
Rice starch, the rice starch that 500rpm magnetic agitations are mixed to get-protein peptides suspension.Rice starch-the albumen that will be obtained
Peptide suspension, it is dry using the micro-fluidic spraying of laboratory level after homogeneous 8min under homogenization pressure 50MPa in high pressure homogenizer
Dry tower, using 75 μm of nozzles, material flow 1.0g/min, hot blast flow velocity 250L/min are set, tower top temperature is controlled as 200
DEG C, corresponding column bottom temperature is 90 DEG C, prepares protein peptides-slow-digestion starch particle.
As shown in Figures 1 to 3, wherein Fig. 1 is untreated rice starch to electron microscopic observation result, and Fig. 2 changes for Typical physical
Property obtain rice starch, Fig. 3 be with the present embodiment method obtain protein peptides-slow-digestion starch particle.As shown in Figure 1, not
Treated rice starch granule is very small, between 5~8 μm, and homogeneous polygonal of granularity, surface is more smooth.By Fig. 2
It is shown, it is modified by Typical physical, high-pressure homogeneous and spray drying treatment rice starch, has adhesion glomeration between particle
Type phenomenon.Although it is also possible to, it is evident that have passed through high-pressure homogeneous and spray drying treatment, its script particle it is small and
Homogeneous polygonal of granularity, does not change.Protein peptides-slow-digestion starch particle that the present embodiment methods described obtains, such as
Shown in Fig. 3, all rice starch granules, which are fused into surface, the particle of depression, and wherein slow-digestion starch content is up to
55.63%.Slow-digestion starch content in the protein peptides-slow-digestion starch particle obtained based on the present embodiment method is and original
Slow-digestion starch content is compared in rice starch, is improved by 10.25% to 55.63%.
Embodiment 2
Grass carp protein peptides refine, and the molecular weight for weighing the sale of 60g Shandong Zhong Shidouqing Bioisystech Co., Ltd is less than
The protein peptides of 2000 dalton, are dissolved in 1000mL distilled water, using molecular weight as the super of 300 dalton and 1000 dalton
Mwco membrane is filtered, carries out ultrafiltration, after the grass carp protein purification peptide solution for obtaining 300-1000 dalton, is obtained after being freeze-dried
Refined grass carp protein peptides, it is standby to store at room temperature.
Weigh the grass carp protein peptides 8g that molecular weight is 300-1000 dalton and be dissolved in 1000mL distilled water, then add 100g
Rice starch, the rice starch that 550rpm magnetic agitations are mixed to get-protein peptides suspension.Rice starch-the albumen that will be obtained
Peptide suspension, it is dry using the micro-fluidic spraying of laboratory level after homogeneous 6min under homogenization pressure 65MPa in high pressure homogenizer
Dry tower, using 75 μm of nozzles, material flow 1.2g/min, hot blast flow velocity 230L/min are set, tower top temperature is controlled as 190
DEG C, corresponding column bottom temperature is 90 DEG C, prepares protein peptides-slow-digestion starch particle.Wherein, the content of protein is 8%, is disappeared slowly
Change content of starch and be up to 54%.Slow-digestion starch contains in the protein peptides-slow-digestion starch particle obtained based on the present embodiment method
Amount, compared with slow-digestion starch content in original rice starch, improved by 10.25% to 54%.
Embodiment 3
Grass carp protein peptides refine, and the molecular weight for weighing the sale of 40g Shandong Zhong Shidouqing Bioisystech Co., Ltd is less than
The protein peptides of 2000 dalton, are dissolved in 1000mL distilled water, using molecular weight as the super of 300 dalton and 1000 dalton
Mwco membrane is filtered, carries out ultrafiltration, after the grass carp protein purification peptide solution for obtaining 300-1000 dalton, is obtained after being freeze-dried
Refined grass carp protein peptides, it is standby to store at room temperature.
Weigh the grass carp protein peptides 15g that molecular weight is 300-1000 dalton and be dissolved in 1000mL distilled water, then add
100g rice starch, the rice starch that 450rpm magnetic agitations are mixed to get-protein peptides suspension.By obtained rice starch-
Protein peptides suspension, in high pressure homogenizer, under homogenization pressure 65MPa, after homogeneous 4min, using the micro-fluidic spray of laboratory level
Mist drying tower, using 75 μm of nozzles, material flow 0.8g/min, hot blast flow velocity 260L/min are set, tower top temperature controls respectively
For 185 DEG C, corresponding column bottom temperature is 85 DEG C, prepares protein peptides-slow-digestion starch particle.Wherein, the content of protein is
15%, slow-digestion starch content is up to 54%.Disappear slowly in the protein peptides-slow-digestion starch particle obtained based on the present embodiment method
Change content of starch, compared with slow-digestion starch content in original rice starch, improved by 10.25% to 54%.
Described above is only the preferred embodiment of the present invention, it is noted that for the ordinary skill people of the art
For member, under the premise without departing from the principles of the invention, some improvements and modifications can also be made, these improvements and modifications also should
It is considered as protection scope of the present invention.