CN107163149A - A kind of preparation method of lotus seed starch albumen composition - Google Patents
A kind of preparation method of lotus seed starch albumen composition Download PDFInfo
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- CN107163149A CN107163149A CN201710347007.4A CN201710347007A CN107163149A CN 107163149 A CN107163149 A CN 107163149A CN 201710347007 A CN201710347007 A CN 201710347007A CN 107163149 A CN107163149 A CN 107163149A
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- 240000002853 Nelumbo nucifera Species 0.000 title claims abstract description 184
- 235000006508 Nelumbo nucifera Nutrition 0.000 title claims abstract description 184
- 235000006510 Nelumbo pentapetala Nutrition 0.000 title claims abstract description 184
- 229920002472 Starch Polymers 0.000 title claims abstract description 116
- 239000008107 starch Substances 0.000 title claims abstract description 116
- 235000019698 starch Nutrition 0.000 title claims abstract description 78
- 239000000203 mixture Substances 0.000 title claims abstract description 56
- 238000002360 preparation method Methods 0.000 title claims abstract description 27
- 239000006228 supernatant Substances 0.000 claims abstract description 41
- 238000006243 chemical reaction Methods 0.000 claims abstract description 28
- 239000000047 product Substances 0.000 claims abstract description 15
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 14
- 239000004382 Amylase Substances 0.000 claims abstract description 11
- 102000013142 Amylases Human genes 0.000 claims abstract description 11
- 108010065511 Amylases Proteins 0.000 claims abstract description 11
- 235000019418 amylase Nutrition 0.000 claims abstract description 11
- 238000001035 drying Methods 0.000 claims abstract description 10
- 239000002253 acid Substances 0.000 claims abstract description 8
- -1 polyphenol compound Chemical class 0.000 claims abstract description 7
- 238000001556 precipitation Methods 0.000 claims abstract description 6
- 238000001976 enzyme digestion Methods 0.000 claims abstract description 5
- 102000004169 proteins and genes Human genes 0.000 claims description 44
- 108090000623 proteins and genes Proteins 0.000 claims description 44
- 239000000243 solution Substances 0.000 claims description 35
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 claims description 27
- 239000007788 liquid Substances 0.000 claims description 22
- 150000001875 compounds Chemical class 0.000 claims description 20
- 102000004190 Enzymes Human genes 0.000 claims description 16
- 108090000790 Enzymes Proteins 0.000 claims description 16
- 229940088598 enzyme Drugs 0.000 claims description 16
- 238000012545 processing Methods 0.000 claims description 15
- CBMPTFJVXNIWHP-UHFFFAOYSA-L disodium;hydrogen phosphate;2-hydroxypropane-1,2,3-tricarboxylic acid Chemical compound [Na+].[Na+].OP([O-])([O-])=O.OC(=O)CC(O)(C(O)=O)CC(O)=O CBMPTFJVXNIWHP-UHFFFAOYSA-L 0.000 claims description 14
- 239000007981 phosphate-citrate buffer Substances 0.000 claims description 14
- 108010073178 Glucan 1,4-alpha-Glucosidase Proteins 0.000 claims description 12
- 102100022624 Glucoamylase Human genes 0.000 claims description 12
- 239000002244 precipitate Substances 0.000 claims description 12
- 238000007873 sieving Methods 0.000 claims description 11
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims description 10
- 238000005119 centrifugation Methods 0.000 claims description 10
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- 238000009413 insulation Methods 0.000 claims description 8
- 102000004139 alpha-Amylases Human genes 0.000 claims description 7
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- 229940024171 alpha-amylase Drugs 0.000 claims description 7
- 239000012153 distilled water Substances 0.000 claims description 7
- 238000010438 heat treatment Methods 0.000 claims description 6
- 239000001257 hydrogen Substances 0.000 claims description 6
- 229910052739 hydrogen Inorganic materials 0.000 claims description 6
- 230000003139 buffering effect Effects 0.000 claims description 2
- OAICVXFJPJFONN-UHFFFAOYSA-N Phosphorus Chemical compound [P] OAICVXFJPJFONN-UHFFFAOYSA-N 0.000 claims 1
- 229910052698 phosphorus Inorganic materials 0.000 claims 1
- 239000011574 phosphorus Substances 0.000 claims 1
- 244000269722 Thea sinensis Species 0.000 abstract 1
- 235000013824 polyphenols Nutrition 0.000 abstract 1
- 230000006798 recombination Effects 0.000 description 15
- 238000005215 recombination Methods 0.000 description 15
- 238000000034 method Methods 0.000 description 12
- 230000000694 effects Effects 0.000 description 11
- 150000002632 lipids Chemical class 0.000 description 11
- 238000005516 engineering process Methods 0.000 description 9
- 238000001179 sorption measurement Methods 0.000 description 8
- 235000011121 sodium hydroxide Nutrition 0.000 description 7
- NBIIXXVUZAFLBC-UHFFFAOYSA-N Phosphoric acid Chemical compound OP(O)(O)=O NBIIXXVUZAFLBC-UHFFFAOYSA-N 0.000 description 6
- 229940111205 diastase Drugs 0.000 description 6
- 239000000843 powder Substances 0.000 description 6
- 230000009286 beneficial effect Effects 0.000 description 5
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- 238000010025 steaming Methods 0.000 description 5
- 239000007853 buffer solution Substances 0.000 description 4
- UFHFLCQGNIYNRP-UHFFFAOYSA-N Hydrogen Chemical compound [H][H] UFHFLCQGNIYNRP-UHFFFAOYSA-N 0.000 description 3
- DGAQECJNVWCQMB-PUAWFVPOSA-M Ilexoside XXIX Chemical compound C[C@@H]1CC[C@@]2(CC[C@@]3(C(=CC[C@H]4[C@]3(CC[C@@H]5[C@@]4(CC[C@@H](C5(C)C)OS(=O)(=O)[O-])C)C)[C@@H]2[C@]1(C)O)C)C(=O)O[C@H]6[C@@H]([C@H]([C@@H]([C@H](O6)CO)O)O)O.[Na+] DGAQECJNVWCQMB-PUAWFVPOSA-M 0.000 description 3
- 239000004743 Polypropylene Substances 0.000 description 3
- 238000010521 absorption reaction Methods 0.000 description 3
- 229910000147 aluminium phosphate Inorganic materials 0.000 description 3
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- 230000000052 comparative effect Effects 0.000 description 3
- 238000010790 dilution Methods 0.000 description 3
- 239000012895 dilution Substances 0.000 description 3
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- 239000001301 oxygen Substances 0.000 description 3
- 229910052760 oxygen Inorganic materials 0.000 description 3
- 229920001155 polypropylene Polymers 0.000 description 3
- 230000008569 process Effects 0.000 description 3
- 239000012460 protein solution Substances 0.000 description 3
- 229910052708 sodium Inorganic materials 0.000 description 3
- 239000011734 sodium Substances 0.000 description 3
- 239000001509 sodium citrate Substances 0.000 description 3
- NLJMYIDDQXHKNR-UHFFFAOYSA-K sodium citrate Chemical compound O.O.[Na+].[Na+].[Na+].[O-]C(=O)CC(O)(CC([O-])=O)C([O-])=O NLJMYIDDQXHKNR-UHFFFAOYSA-K 0.000 description 3
- 229960001790 sodium citrate Drugs 0.000 description 3
- 235000011083 sodium citrates Nutrition 0.000 description 3
- 230000002269 spontaneous effect Effects 0.000 description 3
- 239000000126 substance Substances 0.000 description 3
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- HVCOBJNICQPDBP-UHFFFAOYSA-N 3-[3-[3,5-dihydroxy-6-methyl-4-(3,4,5-trihydroxy-6-methyloxan-2-yl)oxyoxan-2-yl]oxydecanoyloxy]decanoic acid;hydrate Chemical compound O.OC1C(OC(CC(=O)OC(CCCCCCC)CC(O)=O)CCCCCCC)OC(C)C(O)C1OC1C(O)C(O)C(O)C(C)O1 HVCOBJNICQPDBP-UHFFFAOYSA-N 0.000 description 2
- 229930186217 Glycolipid Natural products 0.000 description 2
- 230000008901 benefit Effects 0.000 description 2
- 239000008367 deionised water Substances 0.000 description 2
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- 238000009792 diffusion process Methods 0.000 description 2
- 238000001125 extrusion Methods 0.000 description 2
- 235000013305 food Nutrition 0.000 description 2
- 230000008595 infiltration Effects 0.000 description 2
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- 150000003384 small molecules Chemical class 0.000 description 2
- UFTFJSFQGQCHQW-UHFFFAOYSA-N triformin Chemical compound O=COCC(OC=O)COC=O UFTFJSFQGQCHQW-UHFFFAOYSA-N 0.000 description 2
- 238000002604 ultrasonography Methods 0.000 description 2
- MPDGHEJMBKOTSU-YKLVYJNSSA-N 18beta-glycyrrhetic acid Chemical compound C([C@H]1C2=CC(=O)[C@H]34)[C@@](C)(C(O)=O)CC[C@]1(C)CC[C@@]2(C)[C@]4(C)CC[C@@H]1[C@]3(C)CC[C@H](O)C1(C)C MPDGHEJMBKOTSU-YKLVYJNSSA-N 0.000 description 1
- 235000005979 Citrus limon Nutrition 0.000 description 1
- 244000131522 Citrus pyriformis Species 0.000 description 1
- 241000196324 Embryophyta Species 0.000 description 1
- 229910019142 PO4 Inorganic materials 0.000 description 1
- 235000009754 Vitis X bourquina Nutrition 0.000 description 1
- 235000012333 Vitis X labruscana Nutrition 0.000 description 1
- 240000006365 Vitis vinifera Species 0.000 description 1
- 235000014787 Vitis vinifera Nutrition 0.000 description 1
- 238000002835 absorbance Methods 0.000 description 1
- 230000000844 anti-bacterial effect Effects 0.000 description 1
- 239000007864 aqueous solution Substances 0.000 description 1
- 238000010923 batch production Methods 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 238000013270 controlled release Methods 0.000 description 1
- 230000006378 damage Effects 0.000 description 1
- 238000013461 design Methods 0.000 description 1
- 229940079593 drug Drugs 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 230000005611 electricity Effects 0.000 description 1
- 230000007071 enzymatic hydrolysis Effects 0.000 description 1
- 238000006047 enzymatic hydrolysis reaction Methods 0.000 description 1
- 238000000605 extraction Methods 0.000 description 1
- 239000008236 heating water Substances 0.000 description 1
- 230000003993 interaction Effects 0.000 description 1
- 235000004213 low-fat Nutrition 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 102000035118 modified proteins Human genes 0.000 description 1
- 108091005573 modified proteins Proteins 0.000 description 1
- 229920005615 natural polymer Polymers 0.000 description 1
- NBIIXXVUZAFLBC-UHFFFAOYSA-K phosphate Chemical compound [O-]P([O-])([O-])=O NBIIXXVUZAFLBC-UHFFFAOYSA-K 0.000 description 1
- 239000010452 phosphate Substances 0.000 description 1
- 229920000642 polymer Polymers 0.000 description 1
- 230000020978 protein processing Effects 0.000 description 1
- 230000004044 response Effects 0.000 description 1
- 238000007789 sealing Methods 0.000 description 1
- 229910001220 stainless steel Inorganic materials 0.000 description 1
- 239000010935 stainless steel Substances 0.000 description 1
- 238000003786 synthesis reaction Methods 0.000 description 1
- 238000010998 test method Methods 0.000 description 1
- 238000012360 testing method Methods 0.000 description 1
- 238000000870 ultraviolet spectroscopy Methods 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K19/00—Hybrid peptides, i.e. peptides covalently bound to nucleic acids, or non-covalently bound protein-protein complexes
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P19/00—Preparation of compounds containing saccharide radicals
- C12P19/04—Polysaccharides, i.e. compounds containing more than five saccharide radicals attached to each other by glycosidic bonds
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P19/00—Preparation of compounds containing saccharide radicals
- C12P19/14—Preparation of compounds containing saccharide radicals produced by the action of a carbohydrase (EC 3.2.x), e.g. by alpha-amylase, e.g. by cellulase, hemicellulase
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- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Biochemistry (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- General Health & Medical Sciences (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Microbiology (AREA)
- General Chemical & Material Sciences (AREA)
- General Engineering & Computer Science (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Biotechnology (AREA)
- Medicinal Chemistry (AREA)
- Biophysics (AREA)
- Molecular Biology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Enzymes And Modification Thereof (AREA)
- Cosmetics (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Non-Alcoholic Beverages (AREA)
- Preparation Of Fruits And Vegetables (AREA)
Abstract
The invention provides a kind of preparation method of lotus seed starch albumen composition, comprise the following steps：Lotus seed starch is dissolved in acid solution, amylase is added and carries out 24~48h of enzyme digestion reaction, then regulation system is alkalescence, being then centrifuged for removing supernatant to precipitate, will precipitation washed after remove supernatant, dry and crush and obtain lotus seeds porous-starch；Microwave ultrasonic wave 16~20min of Combined Treatment is used after after phosphatide is mixed with lotus seeds porous-starch, rough lotus seeds porous-starch phosphatide complexes are obtained；Ultra high pressure treatment is carried out after albumen, rough lotus seeds porous-starch phosphatide complexes and water are mixed, it is then centrifuged for removing the lotus seed starch albumen composition that supernatant obtains hygrometric state, then it will crush, sieve after lotus seed starch albumen composition drying to the constant weight of hygrometric state, obtain finished product.The present invention can prepare the high lotus seed starch tea polyphenol compound of complex indexes.
Description
Technical field
The present invention relates to modified protein processing technique field, more particularly to a kind of preparation of lotus seed starch-albumen composition
Method.
Background technology
Starch and protein are the important natural polymerses of two classes, with cheap and easy to get, degradable and good
The characteristics such as biocompatibility.The exploitation of starch and protein resource are all attached great importance in countries in the world for a long time, especially
The modifying and decorating of the two is always study hotspot.Coexisted at both, when some physical and chemical conditions are suitable, be then copolymerized modification
Moieties in phenomenon, i.e. macromolecular can interconnect it is compound, so as to improve and imparting system some unique features
Matter, processing characteristics and and quality characteristic, finally expand its application in food industry and other industry, such as can be used as edible
Film, available for exploitation low-fat food, drug controlled release system etc..
At present, the preparation of starch-albumen composition, mainly there is following 4 kinds of methods：
(1) dry process reaction is by controlling spontaneous Maillard reaction to realize, by starch and albumen powder with certain mass than mixed
It is freeze-dried after conjunction with after deionized water dissolving, lyophilized sample is placed in bottom equipped with saturation KBr solution, and humidity is 79% reaction
In device, in reaction a few hours to several weeks at 60 DEG C；
(2) aqueous solution of wet method reaction starch and protein is reacted under certain condition, and protein and starch press one
Determine mass ratio mixing, the heating water bath grafting of sealing centrifuge tube is placed in after cushioning liquid dilution；
(3) mass fraction starch and protein solution are filled in battery case of the electro synthesis with two stainless steel electrodes, led to
Compound gel is removed after electricity frequently, compound is drying to obtain；
(4) handled after extrusion starch is mixed by a certain percentage with protein using screw machine press, and select suitable
The associated technical parameters such as extrusion temperature, moisture, screw speed.
But the above method is because only with spontaneous Maillard reaction, starch can not be fully combined with albumen, and typically
The particulate component (albumen and lipid) that can be combined with albumen of preparation method starch surface it is less, condition requirement is strict in addition, leads
Cause the recombination rate of obtained starch-albumen composition low, be not easy to the use and processing for the compound later stage.
The content of the invention
It is an object of the invention to overcome drawbacks described above there is provided a kind of preparation method of lotus seed starch-albumen composition,
The recombination rate for lotus seed starch-albumen composition that this method is prepared is high.
In order to solve the above-mentioned technical problem, the technical solution adopted by the present invention is：
The invention provides a kind of preparation method of lotus seed starch-albumen composition, comprise the following steps：
Step 1：Lotus seed starch is dissolved in acid solution, amylase is added and carries out 24~48h of enzyme digestion reaction, then adjust
System is alkalescence, and being then centrifuged for removing supernatant to precipitate, will precipitation washed after remove supernatant, dry and crush and obtain
Lotus seeds porous-starch；
Step 2：After after phosphatide is mixed with the lotus seeds porous-starch of step 1 with microwave-ultrasonic Combined Treatment 16~
20min, obtains rough lotus seeds porous-starch-phosphatide complexes；
Step 3：Superelevation is carried out after lotus seeds porous-starch-phosphatide complexes and water rough obtained by albumen, step 2 are mixed
Pressure processing, is then centrifuged for removing lotus seed starch-albumen composition that supernatant obtains hygrometric state, then by lotus seed starch-egg of hygrometric state
Crush, sieve after white compound drying to constant weight, obtain finished product.
Present invention also offers a kind of preparation method of lotus seed starch-albumen composition, comprise the following steps：
Step 1：Lotus seed starch is dissolved in disodium hydrogen phosphate-citrate buffer solution that pH value is 5.5, in 53~57 DEG C of bars
Heat the complex enzyme that alpha-amylase and glucoamylase are added after 8~12min under part, 24~48h of insulation reaction, followed by
1mol/L sodium hydroxide solution regulation system is alkalescence, then removes supernatant in centrifugation 15min under 5000r/min rotating speed
It must precipitate, supernatant is removed after precipitation is washed, by centrifugation gains in dry to constant weight under 48~52 DEG C of condition of normal pressure
Then crush, sieving obtains lotus seeds porous-starch, wherein lotus seed starch and disodium hydrogen phosphate-lemon acid buffering that pH value is 5.5
The solid-liquid ratio of liquid is 1: 1.5~2.5, and the unit of solid-liquid ratio is g/mL, and sodium hydroxide solution and the pH value of the 1mol/L are 5.5
Disodium hydrogen phosphate-citrate buffer solution volume ratio be 1: 10；
Step 2：After after phosphatide is mixed with the lotus seeds porous-starch of step 1 with microwave-ultrasonic Combined Treatment 16~
20min, obtains rough lotus seeds porous-starch-phosphatide complexes, rough lotus seeds porous-starch-phosphatide is washed with absolute ethyl alcohol multiple
Compound, is then freeze-dried to constant weight, obtains refined lotus seeds porous-starch-phosphatide complexes, wherein, the lotus seeds porous-starch
Mass ratio with phosphatide is 1: 5~7, and it is 150~160W, ultrasound that the condition of microwave-ultrasonic Combined Treatment, which includes microwave power,
Wave power is 350~400W, and temperature is 70~90 DEG C；
Step 3：Lotus seeds porous-starch-phosphatide complexes refined obtained by lotus seed protein and step 2 are dissolved separately in steaming
Mixed liquor is remixed to obtain after distilled water, the phosphate buffer solution that pH is 4.7 is added into mixed liquor, then will be added with pH
The mixed liquor of 4.7 phosphate buffer solution carries out batch (-type) 20~30min of ultra high pressure treatment, then is washed, and is then centrifuged for
Lotus seed starch-albumen composition that supernatant obtains hygrometric state is removed, then by lotus seed starch-albumen composition of hygrometric state in 48~52
Dried under DEG C condition of normal pressure crushed to constant weight, sieving, obtain finished product, wherein, the lotus seeds porous-starch-phosphatide complexes
Be 1: 5~7 with the mass ratio of the mixing of lotus seed protein, the condition of the batch (-type) ultra high pressure treatment include pressure be 200~
250MPa, temperature is 60~80 DEG C, often runs 5min and rests 30s.
The beneficial effects of the present invention are：(1) using amylase by common lotus seed starch be prepared into lotus seeds porous-starch come
Increase the surface area of starch, using microwave-ultrasonic Combined Treatment auxiliary porous starch adsorption phosphatide to increase starch surface
Lipid components, and the surface hydrophobicity effect of protein is improved using non-thermal technology super-pressure, greatly increase starch and albumen
Complex indexes, prepares lotus seed starch-albumen composition of high recombination rate；(2) preparation method of the invention is not grasped using high temperature
Make, can effectively keep the structure of starch and albumen, the performance for the lotus seed starch-albumen composition prepared more preferably, it is convenient after
Continuous use and processing.
Embodiment
To describe the technology contents of the present invention in detail, feature, the objects and the effects being constructed, below in conjunction with embodiment
It is explained in detail.
The design of most critical of the present invention is：Microwave-ultrasonic bar is placed in by the lotus seeds porous-starch for preparing enzyme process
Part gets off to adsorb phosphatide to increase adsorbance of the starch to protein, and makes starch and albumen phase interaction using super-pressure effect
With, and then the high lotus seed starch-albumen composition of recombination rate is made.
A kind of preparation method of lotus seed starch-albumen composition, comprises the following steps：
Step 1：Lotus seed starch is dissolved in acid solution, amylase is added and carries out 24~48h of enzyme digestion reaction, then adjust
System is alkalescence, and being then centrifuged for removing supernatant to precipitate, will precipitation washed after remove supernatant, dry and crush and obtain
Lotus seeds porous-starch；
Step 2：After after phosphatide is mixed with the lotus seeds porous-starch of step 1 with microwave-ultrasonic Combined Treatment 16~
20min, obtains rough lotus seeds porous-starch-phosphatide complexes；
Step 3：Superelevation is carried out after lotus seeds porous-starch-phosphatide complexes and water rough obtained by albumen, step 2 are mixed
Pressure processing, is then centrifuged for removing lotus seed starch-albumen composition that supernatant obtains hygrometric state, then by lotus seed starch-egg of hygrometric state
Crush, sieve after white compound drying to constant weight, obtain finished product.
The present invention operation principle and process be：Because warp is applicants have found that lipid is conducive to starch particle surface to inhale
Attached more protein, and the phosphatide of polarity can adsorb more protein compared to neutral glycolipid, triglyceride, so,
Lotus seed starch is prepared into porous-starch by certain condition enzymolysis, shallow lake is increased using the bigger advantage of porous-starch surface area
The lipid content on powder surface, and then increase absorption of the starch to albumen.Microwave-ultrasonic Combined Treatment auxiliary porous starch adsorption
Phosphatide so that pure phosphatide is entered inside porous-starch by infiltration, diffusion, the lipid components on increase starch surface, with
Beneficial to the more protein of starch adsorption.Super-pressure is not being destroyed under the premise of small-molecule substance as non-thermal technology
Influence is produced on protein structure and property, the surface hydrophobicity effect of protein is greatly enhanced, obtains high compound with reference to starch
The compound of index (high recombination rate).
It was found from foregoing description, the beneficial effects of the present invention are：(1) common lotus seed starch is prepared into using amylase
Lotus seeds porous-starch increases the surface area of starch, using microwave-ultrasonic Combined Treatment auxiliary porous starch adsorption phosphatide with
Increase the lipid components on starch surface, and the surface hydrophobicity effect of protein is improved using non-thermal technology super-pressure, increase
Plus the complex indexes of starch and albumen, prepare lotus seed starch-albumen composition of high recombination rate；(2) preparation side of the invention
Method does not use high-temperature operation, can effectively keep the structure of starch and albumen, the property for the lotus seed starch-albumen composition prepared
Subsequent use and processing more preferably can be facilitated.
Further, the acid solution of the step 1 is disodium hydrogen phosphate-citrate buffer solution that pH value is 5.5, described
Amylase is the complex enzyme of alpha-amylase and glucoamylase, and the enzyme activity of alpha-amylase is 10 000U wherein in complex enzyme
g-1, the enzyme activity of glucoamylase is 3 700Ug-1。
Seen from the above description, selection pH value can be with as enzymatic hydrolysis condition for 5.5 disodium hydrogen phosphate-citrate buffer solution
Reduce influence of the fluctuation of pH value to enzyme digestion reaction, select the complex enzyme of alpha-amylase and glucoamylase to cause lotus seed starch
Digested more abundant.
Further, the concrete operations of the step 1 are：Lotus seed starch is dissolved in disodium hydrogen phosphate-lemon that pH value is 5.5
In lemon acid buffer, in addition mesophilicα-diastase (10 000Ug after 8~12min of heating under the conditions of 53~57 DEG C-1) and Portugal
Grape saccharogenic amylase (3 700Ug-1) complex enzyme, 24~48h of insulation reaction, followed by 1mol/L sodium hydroxide solution adjust
Section system is alkalescence, and being then centrifuged for removal supernatant must precipitate, and will precipitate and supernatant is removed after being washed, and drying is right to constant weight
Crush afterwards, sieving obtains lotus seeds porous-starch, wherein lotus seed starch and disodium hydrogen phosphate-citrate buffer solution that pH value is 5.5
Solid-liquid ratio be 1: 1.5~2.5, the unit of solid-liquid ratio is g/mL.
Seen from the above description, above-mentioned condition contributes to enzymolysis more abundant and improves enzymolysis efficiency, while molten using highly basic
PH is with enzymolysis reaction for liquid --- sodium hydroxide solution regulation system, easy to use.
Further, in step 2, the condition of microwave-ultrasonic Combined Treatment includes：Microwave power is 150~160W, is surpassed
Acoustic power is 350~400W, and temperature is 70~90 DEG C.
Seen from the above description, the condition of microwave-ultrasonic batch process is controlled to be conducive to lotus seeds many in above range
The more phosphatide of hole starch adsorption and the fastness of increase absorption, and then it is easy to the later stage to adsorb greater protein, further improve lotus seeds
The recombination rate of starch-albumen composition.
Further, between step 2 and step 3 also using absolute ethyl alcohol wash rough lotus seeds porous-starch-
Phosphatide complexes, are then freeze-dried to constant weight, obtain refined lotus seeds porous-starch-phosphatide complexes.
Seen from the above description, using free phosphatide and lotus seeds it is porous-phosphatide complexes absolute ethyl alcohol solubility
Difference, the phosphatide that will not dissociate is washed away, it is to avoid free phosphatide is directly compound with albumen, to improve lotus seed starch-albumen composition
Purity.
Further, in step 3, the ultra high pressure treatment is at batch (-type) ultra high pressure treatment, the batch (-type) super-pressure
The condition of reason includes：Pressure is 200~250MPa, and temperature is 60~80 DEG C, and processing time is 20~30min, often runs 5min
Rest 30s.
Wherein, processing time is each run time sum.
Seen from the above description, use batch (-type) ultra high pressure treatment and control its treatment conditions not only can be with above range
Improve the follow-up recombination rate of surface hydrophobicity effect increase of albumen, moreover it is possible to play bactericidal effect.
Further, in step 3, the albumen is lotus seed protein, the lotus seeds porous-starch-phosphatide complexes and lotus seeds
The mass ratio of the mixing of albumen is 1: 5~7.
Seen from the above description, using protein of the lotus seed protein as compound, lotus seed starch-albumen is further improved
The recombination rate of compound.
Further, the drying of step 1 and step 3 is to be dried under 48~52 DEG C of condition of normal pressure.
Seen from the above description, the too high destruction starch of temperature is avoided and compound in being dried under 48~52 DEG C of condition of normal pressure
The structure of thing is to keep the stability of the performance of compound.
The present invention also provides a kind of preparation method of lotus seed starch-albumen composition, comprises the following steps：
Step 1：Lotus seed starch is dissolved in disodium hydrogen phosphate-citrate buffer solution that pH value is 5.5, in 53~57 DEG C of bars
Heated under part and mesophilicα-diastase (10 000Ug are added after 8~12min-1) and glucoamylase (3 700Ug-1)
Complex enzyme, 24~48h of insulation reaction is alkalescence, Ran Houyu followed by 1mol/L sodium hydroxide solution regulation system
15min removal supernatants are centrifuged under 5000r/min rotating speed to be precipitated, and will be precipitated and be removed supernatant after being washed, and will centrifuge
In being dried under 48~52 DEG C of condition of normal pressure to constant weight, then crushing, sieving obtain lotus seeds porous-starch to gains, and wherein lotus seeds form sediment
The solid-liquid ratio for disodium hydrogen phosphate-citrate buffer solution that powder is 5.5 with pH value is 1: 1.5~2.5, and the unit of solid-liquid ratio is g/
The volume ratio for disodium hydrogen phosphate-citrate buffer solution that mL, the 1mol/L sodium hydroxide solution are 5.5 with pH value is 1:
10；
Step 2：After after phosphatide is mixed with the lotus seeds porous-starch of step 1 with microwave-ultrasonic Combined Treatment 16~
20min, obtains rough lotus seeds porous-starch-phosphatide complexes, rough lotus seeds porous-starch-phosphatide is washed with absolute ethyl alcohol multiple
Compound, is then freeze-dried to constant weight, obtains refined lotus seeds porous-starch-phosphatide complexes, wherein, the lotus seeds porous-starch
Mass ratio with phosphatide is 1: 5~7, and it is 150~160W, ultrasound that the condition of microwave-ultrasonic Combined Treatment, which includes microwave power,
Wave power is 350~400W, and temperature is 70~90 DEG C；
Step 3：Lotus seeds porous-starch-phosphatide complexes refined obtained by lotus seed protein and step 2 are dissolved separately in steaming
Mixed liquor is remixed to obtain after distilled water, the phosphate buffer solution that pH is 4.7 is added into mixed liquor, then will be added with pH
The mixed liquor of 4.7 phosphate buffer solution carries out batch (-type) 20~30min of ultra high pressure treatment, then is washed, and is then centrifuged for
Lotus seed starch-albumen composition that supernatant obtains hygrometric state is removed, then by lotus seed starch-albumen composition of hygrometric state in 48~52
Dried under DEG C condition of normal pressure crushed to constant weight, sieving, obtain finished product, wherein, the lotus seeds porous-starch-phosphatide complexes
Be 1: 5~7 with the mass ratio of the mixing of lotus seed protein, the condition of the batch (-type) ultra high pressure treatment include pressure be 200~
250MPa, temperature is 60~80 DEG C, often runs 5min and rests 30s.
The present invention operation principle and process be：Because warp is applicants have found that lipid is conducive to starch particle surface to inhale
Attached more protein, and the phosphatide of polarity can adsorb more protein compared to neutral glycolipid, triglyceride, so,
Lotus seed starch is prepared into porous-starch by certain condition enzymolysis, shallow lake is increased using the bigger advantage of porous-starch surface area
The lipid content on powder surface, and then increase absorption of the starch to albumen.Microwave-ultrasonic Combined Treatment auxiliary porous starch adsorption
Phosphatide so that pure phosphatide is entered inside porous-starch by infiltration, diffusion, the lipid components on increase starch surface, with
Beneficial to the more protein of starch adsorption.Super-pressure is not being destroyed under the premise of small-molecule substance as non-thermal technology
Influence is produced on protein structure and property, the surface hydrophobicity effect of protein is greatly enhanced, obtains high compound with reference to starch
The compound of index (high recombination rate).
It was found from foregoing description, the beneficial effects of the present invention are：(1) common lotus seed starch is prepared into using amylase
Lotus seeds porous-starch increases the surface area of starch, using microwave-ultrasonic Combined Treatment auxiliary porous starch adsorption phosphatide with
Increase the lipid components on starch surface, and the surface hydrophobicity effect of protein is improved using non-thermal technology super-pressure, increase
Plus the complex indexes of starch and albumen, prepare lotus seed starch-albumen composition of high recombination rate；(2) preparation side of the invention
Method does not use high-temperature operation, can effectively keep the structure of starch and albumen, the property for the lotus seed starch-albumen composition prepared
Subsequent use and processing more preferably can be facilitated.
Embodiment 1
1 materials and methods
1.1 material
Lotus seed protein, lotus seed starch, disodium hydrogen phosphate-citrate buffer solution, mesophilicα-diastase (10 000Ug-1) with
Glucoamylase (3 700Ug-1), l mol/L sodium hydroxides, phosphatide, absolute ethyl alcohol, pH for 4.7 phosphate-buffered it is molten
Liquid
1.2 key instrument
DK-S24 type electric-heated thermostatic water baths, the upper grand experimental facilities Co., Ltd of Nereid；
TU-1901 ultraviolet-visible spectrophotometers, Beijing Puxi General Instrument Co., Ltd；
5L-HPP-600MPa type ultra high pressure treatment devices, packet header section hair high pressure science and technology limited Company；
SL-SM type microwave ultrasonic wave association response systems, Nanjing following current instrument manufacturing Co., Ltd；
TG16-WS type centrifuges, Changzhou Jintan high-tech instrument plant；
BAS224S type electronic analytical balances, Sai Duolisi scientific instrument (Beijing) Co., Ltd.
1.3 test method
1.3.1 a kind of preparation method of lotus seed starch-albumen composition, comprises the following steps：
Step 1：It is 5.5 phosphoric acid hydrogen two that 25g lotus seed starch, which is weighed, in 250mL triangular flasks, adding 50mL pH value
In sodium-citrate buffer solution, starch emulsion suspension liquid is configured to, in addition mesophilicα-diastase (10 after heating 10min under the conditions of 55 DEG C
000U·g-1) and glucoamylase (3 700Ug-1) complex enzyme, insulation reaction 24h is subsequently added into 1mol/L hydrogen-oxygen
Change sodium solution 5mL with terminating reaction, then removing supernatant in centrifugation 15min under 5000r/min rotating speed must precipitate, and will precipitate
Supernatant is removed after being washed, then crushing, sieving are obtained in being dried under 50 DEG C of condition of normal pressure to constant weight by centrifugation gains
Lotus seeds porous-starch；
Step 2：Joined after after phosphatide is mixed with the lotus seeds porous-starch of step 1 by 5: 1 mass ratio with microwave-ultrasonic
Conjunction processing (microwave power of microwave-ultrasonic Combined Treatment is 150W, and ultrasonic power is 350W, and temperature is 70 DEG C) 16min,
Rough lotus seeds porous-starch-phosphatide complexes are obtained, rough lotus seeds porous-starch-phosphatide complexes are washed with absolute ethyl alcohol,
Then it is freeze-dried to constant weight, obtains refined lotus seeds porous-starch-phosphatide complexes；
Step 3：Lotus seeds porous-starch-phosphatide complexes refined obtained by lotus seed protein and step 2 are dissolved separately in steaming
Mixed liquor is remixed to obtain after distilled water, pH is added into mixed liquor and is diluted for 4.7 phosphate buffer solution, polypropylene is placed in
Sealed in vacuum bag with vacuum packing machine, progress batch (-type) ultra high pressure treatment (batch (-type) ultra high pressure treatment is put into after fully shaking up
Pressure be 200MPa, temperature is 60 DEG C, often runs 5min and rests 30s) 20min, then washed, be then centrifuged for removing supernatant
Liquid obtains lotus seed starch-albumen composition of hygrometric state, then by lotus seed starch-albumen composition of hygrometric state under 50 DEG C of condition of normal pressure
Dry and crush, sieve to constant weight, obtain finished product, wherein, the lotus seeds porous-starch-phosphatide complexes and lotus seed protein
The mass ratio of mixing is 1: 5.
1.3.2 the measure of product complex indexes (recombination rate)
By the amount for determining the lotus seed protein in centrifuged supernatant.With the lotus in the amount and supernatant of initial lotus seed protein
The difference of sub- protein content can calculate the recombination rate of polymer.The concentration mensuration of lotus seed protein in supernatant：
Solution centrifugal after ultra high pressure treatment is obtained into supernatant, takes 1mL supernatants to be settled to 100mL with distilled water, to mark
Quasi- protein solution does calibration standard quantitative protein, is measured and is absorbed at 278nm with spectrophotometric.
The complex indexes of lotus seed starch-albumen composition is calculated according to following formula：
CI=(M initial-M supernatant)/M initial × 100；
In formula:CI is the complex indexes of lotus seed starch-albumen composition；M initial are the quality of initial lotus seed protein；
M supernatant are the quality of lotus seed protein in supernatant.
Wherein protein content M (mg)=A × V × d/SLOPEstd × 103；
A is sample test absorbance in formula；V is sample extraction volume；D is dilution gfactor, and usually 1mL is diluted to
100mL, i.e. dilution gfactor are 100；SLOPEstd is the slope of protein solution standard curve.
Measured result is repeated three times, and takes average, and the measurement result of product complex indexes is shown in Table 1.
Embodiment 2
Other be the same as Examples 1, difference is the preparation of lotus seed starch-albumen composition, specific as follows：
Step 1：It is 5.5 phosphoric acid hydrogen two that 25g lotus seed starch, which is weighed, in 250mL triangular flasks, adding 50mL pH value
In sodium-citrate buffer solution, starch emulsion suspension liquid is configured to, in addition mesophilicα-diastase (10 after heating 10min under the conditions of 55 DEG C
000U·g-1) and glucoamylase (3 700Ug-1) complex enzyme, insulation reaction 24h is subsequently added into 1mol/L hydrogen-oxygen
Change sodium solution 5mL with terminating reaction, then removing supernatant in centrifugation 15min under 5000r/min rotating speed must precipitate, and will precipitate
Supernatant is removed after being washed, then crushing, sieving are obtained in being dried under 50 DEG C of condition of normal pressure to constant weight by centrifugation gains
Lotus seeds porous-starch；
Step 2：Joined after after phosphatide is mixed with the lotus seeds porous-starch of step 1 by 6: 1 mass ratio with microwave-ultrasonic
Conjunction processing (microwave power of microwave-ultrasonic Combined Treatment is 155W, and ultrasonic power is 375W, and temperature is 80 DEG C) 18min,
Rough lotus seeds porous-starch-phosphatide complexes are obtained, rough lotus seeds porous-starch-phosphatide complexes are washed with absolute ethyl alcohol,
Then it is freeze-dried to constant weight, obtains refined lotus seeds porous-starch-phosphatide complexes；
Step 3：Lotus seeds porous-starch-phosphatide complexes refined obtained by lotus seed protein and step 2 are dissolved separately in steaming
Mixed liquor is remixed to obtain after distilled water, pH is added into mixed liquor and is diluted for 4.7 phosphate buffer solution, polypropylene is placed in
Sealed in vacuum bag with vacuum packing machine, progress batch (-type) ultra high pressure treatment (batch (-type) ultra high pressure treatment is put into after fully shaking up
Pressure be 225MPa, temperature is 70 DEG C, often runs 5min and rests 30s) 25min, then washed, be then centrifuged for removing supernatant
Liquid obtains lotus seed starch-albumen composition of hygrometric state, then by lotus seed starch-albumen composition of hygrometric state under 50 DEG C of condition of normal pressure
Dry and crush, sieve to constant weight, obtain finished product, wherein, the lotus seeds porous-starch-phosphatide complexes and lotus seed protein
The mass ratio of mixing is 1: 6.
The evaluation method be the same as Example 1 of complex indexes, the measurement result of product complex indexes is shown in Table 1.
Embodiment 3
Step 1：It is 5.5 phosphoric acid hydrogen two that 25g lotus seed starch, which is weighed, in 250mL triangular flasks, adding 50mL pH value
In sodium-citrate buffer solution, starch emulsion suspension liquid is configured to, in addition mesophilicα-diastase (10 after heating 10min under the conditions of 55 DEG C
000U·g-1) and glucoamylase (3 700Ug-1) complex enzyme, insulation reaction 24h is subsequently added into 1mol/L hydrogen-oxygen
Change sodium solution 5mL with terminating reaction, then removing supernatant in centrifugation 15min under 5000r/min rotating speed must precipitate, and will precipitate
Supernatant is removed after being washed, then crushing, sieving are obtained in being dried under 50 DEG C of condition of normal pressure to constant weight by centrifugation gains
Lotus seeds porous-starch；
Step 2：Joined after after phosphatide is mixed with the lotus seeds porous-starch of step 1 by 7: 1 mass ratio with microwave-ultrasonic
Conjunction processing (microwave power of microwave-ultrasonic Combined Treatment is 160W, and ultrasonic power is 400W, and temperature is 90 DEG C) 20min,
Rough lotus seeds porous-starch-phosphatide complexes are obtained, rough lotus seeds porous-starch-phosphatide complexes are washed with absolute ethyl alcohol,
Then it is freeze-dried to constant weight, obtains refined lotus seeds porous-starch-phosphatide complexes；
Step 3：Lotus seeds porous-starch-phosphatide complexes refined obtained by lotus seed protein and step 2 are dissolved separately in steaming
Mixed liquor is remixed to obtain after distilled water, pH is added into mixed liquor and is diluted for 4.7 phosphate buffer solution, polypropylene is placed in
Sealed in vacuum bag with vacuum packing machine, progress batch (-type) ultra high pressure treatment (batch (-type) ultra high pressure treatment is put into after fully shaking up
Pressure be 250MPa, temperature is 80 DEG C, often runs 5min and rests 30s) 30min, then washed, be then centrifuged for removing supernatant
Liquid obtains lotus seed starch-albumen composition of hygrometric state, then by lotus seed starch-albumen composition of hygrometric state under 50 DEG C of condition of normal pressure
Dry and crush, sieve to constant weight, obtain finished product, wherein, the lotus seeds porous-starch-phosphatide complexes and lotus seed protein
The mass ratio of mixing is 1: 7.
The evaluation method be the same as Example 1 of complex indexes, the measurement result of product complex indexes is shown in Table 1.
Comparative example
It is prepared by the dry process reaction of starch-albumen composition：
By controlling spontaneous Maillard reaction to realize.Starch and albumen powder are pressed 1:Deionized water dissolving is used after 7 mixing
It is adjusted to 6% (m/V)) it is freeze-dried afterwards.Lyophilized sample is placed in bottom equipped with saturation KBr solution, humidity in 79% reactor,
Xeothermic reaction controlling continues 5d in 60 DEG C of progress, reaction.
The evaluation method be the same as Example 1 of complex indexes, the measurement result of product complex indexes is shown in Table 1.
The measurement result of 1 lotus seed starch of table-albumen composition complex indexes (recombination rate)
| Compound | Complex indexes |
| Embodiment 1 | 52.76% |
| Embodiment 2 | 58.16% |
| Embodiment 3 | 60.22% |
| Comparative example | 29.30% |
As shown in Table 1, lotus seed starch-albumen composition that prepared by the present invention is improved compared to comparative example, its complex indexes
1 times.
In summary, the preparation method of lotus seed starch-albumen composition that the present invention is provided, using amylase by common lotus
Sub- starch is prepared into lotus seeds porous-starch to increase the surface area of starch, is formed sediment using microwave-ultrasonic Combined Treatment auxiliary porous
Powder adsorbs phosphatide to increase the lipid components on starch surface, and utilizes the surface hydrophobicity of non-thermal technology super-pressure raising protein
Effect, greatly increases the complex indexes of starch and albumen, prepares lotus seed starch-albumen composition of high recombination rate；The present invention
Preparation method do not use high-temperature operation, can effectively keep the structure of starch and albumen, the lotus seed starch-albumen prepared is multiple
The performance of compound more preferably, facilitates subsequent use and processing.
Embodiments of the invention are the foregoing is only, are not intended to limit the scope of the invention, it is every to utilize this hair
Equivalent structure or equivalent flow conversion that bright specification is made, or other related technical fields are directly or indirectly used in,
Similarly it is included within the scope of the present invention.
Claims (9)
1. a kind of preparation method of lotus seed starch-albumen composition, it is characterised in that comprise the following steps：
Step 1：Lotus seed starch is dissolved in acid solution, amylase is added and carries out 24~48h of enzyme digestion reaction, then regulation system
For alkalescence, being then centrifuged for removing supernatant to precipitate, will precipitation washed after remove supernatant, dry and crush and obtain lotus seeds
Porous-starch；
Step 2：Microwave-ultrasonic 16~20min of Combined Treatment is used after after phosphatide is mixed with the lotus seeds porous-starch of step 1,
Obtain rough lotus seeds porous-starch-phosphatide complexes；
Step 3：Carried out after lotus seeds porous-starch-phosphatide complexes and water rough obtained by albumen, step 2 are mixed at super-pressure
Reason, is then centrifuged for removing lotus seed starch-albumen composition that supernatant obtains hygrometric state, then that lotus seed starch-albumen of hygrometric state is multiple
Crush, sieve after compound drying to constant weight, obtain finished product.
2. the preparation method of lotus seed starch-albumen composition according to claim 1, it is characterised in that the step 1
Acid solution is disodium hydrogen phosphate-citrate buffer solution that pH value is 5.5, and the amylase is alpha-amylase and glucoamylase
The complex enzyme of enzyme.
3. the preparation method of lotus seed starch-albumen composition according to claim 1, it is characterised in that the step 1
Concrete operations are：Lotus seed starch is dissolved in disodium hydrogen phosphate-citrate buffer solution that pH value is 5.5, in 53~57 DEG C of conditions
The complex enzyme of addition alpha-amylase and glucoamylase after 8~12min of lower heating, 24~48h of insulation reaction, followed by
1mol/L sodium hydroxide solution regulation system is alkalescence, and being then centrifuged for removal supernatant must precipitate, and will precipitate after being washed
Supernatant is removed, dries to constant weight and then crushing, sieving and obtains lotus seeds porous-starch, wherein lotus seed starch and pH value are 5.5
The solid-liquid ratio of disodium hydrogen phosphate-citrate buffer solution is 1: 1.5~2.5, and the unit of solid-liquid ratio is g/mL.
4. the preparation method of lotus seed starch-albumen composition according to claim 1, it is characterised in that micro- in step 2
The condition of ripple-ultrasonic combined processing includes：Microwave power is 150~160W, and ultrasonic power is 350~400W, and temperature is
70~90 DEG C.
5. the preparation method of lotus seed starch-albumen composition according to claim 1, it is characterised in that in step 2 and step
Rough lotus seeds porous-starch-phosphatide complexes are also washed between rapid 3 using absolute ethyl alcohol, are then freeze-dried to perseverance
Weight, obtains refined lotus seeds porous-starch-phosphatide complexes.
6. the preparation method of lotus seed starch-albumen composition according to claim 1, it is characterised in that in step 3, institute
Ultra high pressure treatment is stated for batch (-type) ultra high pressure treatment, the condition of the batch (-type) ultra high pressure treatment includes：Pressure be 200~
250MPa, temperature is 60~80 DEG C, and processing time is 20~30min, often runs 5min and rests 30s.
7. the preparation method of lotus seed starch-albumen composition according to claim 1, it is characterised in that in step 3, institute
Albumen is stated for lotus seed protein, the mass ratio of the mixing of the lotus seeds porous-starch-phosphatide complexes and lotus seed protein is 1: 5~7.
8. the preparation method of lotus seed starch-albumen composition according to claim 1, it is characterised in that step 1 and step
3 drying is to be dried under 48~52 DEG C of condition of normal pressure.
9. a kind of preparation method of lotus seed starch-albumen composition, it is characterised in that comprise the following steps：
Step 1：Lotus seed starch is dissolved in disodium hydrogen phosphate-citrate buffer solution that pH value is 5.5, under the conditions of 53~57 DEG C
The complex enzyme of addition alpha-amylase and glucoamylase after 8~12min of heating, 24~48h of insulation reaction, followed by
1mol/L sodium hydroxide solution regulation system is alkalescence, then removes supernatant in centrifugation 15min under 5000r/min rotating speed
It must precipitate, supernatant is removed after precipitation is washed, by centrifugation gains in dry to constant weight under 48~52 DEG C of condition of normal pressure
Then crush, sieving obtains lotus seeds porous-starch, wherein lotus seed starch and disodium hydrogen phosphate-lemon acid buffering that pH value is 5.5
The solid-liquid ratio of liquid is 1: 1.5~2.5, and the unit of solid-liquid ratio is g/mL, and sodium hydroxide solution and the pH value of the 1mol/L are 5.5
Disodium hydrogen phosphate-citrate buffer solution volume ratio be 1: 10；
Step 2：Microwave-ultrasonic 16~20min of Combined Treatment is used after after phosphatide is mixed with the lotus seeds porous-starch of step 1,
Rough lotus seeds porous-starch-phosphatide complexes are obtained, rough lotus seeds porous-starch-phosphatide complexes are washed with absolute ethyl alcohol,
Then it is freeze-dried to constant weight, obtains refined lotus seeds porous-starch-phosphatide complexes, wherein, the lotus seeds porous-starch and phosphorus
The mass ratio of fat is 1: 5~7, and it is 150~160W, ultrasonic wave work(that the condition of microwave-ultrasonic Combined Treatment, which includes microwave power,
Rate is 350~400W, and temperature is 70~90 DEG C；
Step 3：Lotus seeds porous-starch-phosphatide complexes refined obtained by lotus seed protein and step 2 are dissolved separately in distilled water
After remix to obtain mixed liquor, the phosphate buffer solution that pH is 4.7 is added into mixed liquor, will be then 4.7 added with pH
The mixed liquor of phosphate buffer solution carries out batch (-type) 20~30min of ultra high pressure treatment, then is washed, and is then centrifuged in removal
Clear liquid obtains lotus seed starch-albumen composition of hygrometric state, then by lotus seed starch-albumen composition of hygrometric state in 48~52 DEG C of normal pressures
Under the conditions of dry to crushing after constant weight, sieving, obtain finished product, wherein, the lotus seeds porous-starch-phosphatide complexes and lotus seeds
The mass ratio of the mixing of albumen is 1: 5~7, and it is 200~250MPa, temperature that the condition of the batch (-type) ultra high pressure treatment, which includes pressure,
Spend for 60~80 DEG C, often run 5min and rest 30s.
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| CN111034807A (en) * | 2019-12-18 | 2020-04-21 | 安徽农业大学 | Method for preparing α -albumin-rich cheese by adopting ultrahigh pressure auxiliary tangential membrane filtration |
| CN111165535A (en) * | 2020-03-18 | 2020-05-19 | 河南省金米郎食品有限公司 | Quinoa fried dough twists and preparation method thereof |
| CN111184178A (en) * | 2020-03-18 | 2020-05-22 | 河南牧业经济学院 | Resistant starch-containing product, preparation method and application thereof |
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| CN109259234A (en) * | 2018-11-19 | 2019-01-25 | 福建农林大学 | It is a kind of using resistant starch as the procyanidine microcapsules and preparation method of wall material |
| CN111034807A (en) * | 2019-12-18 | 2020-04-21 | 安徽农业大学 | Method for preparing α -albumin-rich cheese by adopting ultrahigh pressure auxiliary tangential membrane filtration |
| CN111165535A (en) * | 2020-03-18 | 2020-05-19 | 河南省金米郎食品有限公司 | Quinoa fried dough twists and preparation method thereof |
| CN111184178A (en) * | 2020-03-18 | 2020-05-22 | 河南牧业经济学院 | Resistant starch-containing product, preparation method and application thereof |
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| CN114752440A (en) * | 2022-02-15 | 2022-07-15 | 河北中烟工业有限责任公司 | Lotus seed Maillard reactant, preparation method thereof and cigarette feeding liquid |
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