CN1077990A - The method of anti-root knot nematode - Google Patents
The method of anti-root knot nematode Download PDFInfo
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- CN1077990A CN1077990A CN93104410A CN93104410A CN1077990A CN 1077990 A CN1077990 A CN 1077990A CN 93104410 A CN93104410 A CN 93104410A CN 93104410 A CN93104410 A CN 93104410A CN 1077990 A CN1077990 A CN 1077990A
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
- C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
- C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
- C12N15/8279—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance
- C12N15/8285—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance for nematode resistance
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/415—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from plants
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/10—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in agriculture
- Y02A40/146—Genetically Modified [GMO] plants, e.g. transgenic plants
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Abstract
The invention provides a kind of plant of anti-plant root-knot nematode infringement, the identification gene of this plant is expressed in the nematode infection site of infecting plant.Separate the promotor that infects locus gene, exist coding root knot nematode to be infected the sequence of deleterious molecule in this promotor.Further transform plant with described structure chimeric gene.
Description
The present invention relates to the method that anti-plant root-knot nematode infects the harmful effect that causes.
Root knot nematode (Meloidogyne spp) is the main pathogens of many farm crop such as greengrocery plant, leguminous plant, tobacco, tomato, watermelon, grape, peanut and cotton for example.
Chemical prevention, cultivation practices and use pest-resistant cultivar are to prevent and treat the main means that nematode is adopted at present, and often fully utilize these methods to root-knot nematode resistant.Because the protection that these customary methods provide farm crop not enough, so also need the method for control nematode is improved.The problem that the use of nematocides exists ambient condition, and also be not always effective.Cultural control has the potential loss of several respects to the grower.The root knot nematode host range is wide, and this has just limited and can obtain satisfied economically non-host crop.Effective pest-resistant Cultivar usually is difficult for obtaining, and the grower can actual those Cultivars of using be a susceptible Cultivar under low root knot nematode density often.In addition, resistivity also can go down in the high temperature edaphic condition as the torrid zone and subtropical environment.
When root knot nematode infringement roots of plants, it passes the intercellular substance, reaches the meristematic tissue of root, and nematode is injected the meristematic cell position by stylet with the glandulae pharyngeae juice, the normal development of cell is damaged, and the generation nuclear fission, acellular division produces the syncyte that is called " giant cells " thus, along with cytomegalic formation, cell expansion on every side is called as " hypertrophy cell ", and this cell is not that nematode is used the acupuncture directtissima.Giant cells and hypertrophy cell have on every side constituted the site of infecting of root knot nematode together.Viewedly infecting the knot that forms on the root, is the cell composition of the giant cells that produces of this nematode infections and the overgrowth that thereupon produces.The mechanism that giant cells produces is similar in all susceptible plant varieties.
Bring out along with cytomegalic, the root knot nematode that the giant cells that relies on nematode to cause is lived has lost the ability that moves, and nematode is fed growth and breeding in the place of life.
On disclosed international monopoly WO92/04453 number, disclosed the method for preventing and treating of root pocket nematode.The method that relevant mRNA from potato root pocket nematode obtains cDNA is disclosed in " genetic expression in the roots of plants of nematode infections " literary composition being entitled as of delivering people such as Gurr (1991).
The invention provides a kind of method of producing the root knot nematode resistance plant, wherein, with regard to the plant of being infected by root knot nematode, discerned the gene of the excessive growth cell that is expressed in plant root knot giant cells and/or follows, the promotor of described gene and encoding sequence merge so that the chimeric gene of coding molecule to be provided.This gene is harmful to one or more 1. root knot giant cellss, 2. root knot excessive growth cell and 3. root knot nematodes, and further described chimeric gene is transferred in the plant.
The method according to this invention is given the resistance plant of plant antagonism root knot nematode, and for example the greengrocery plant eats leguminous plant, tobacco, drinkable water fruit plant, edible nut class plant and cotton.Radix Dauci Sativae and tomato are respectively the example of its greengrocery plant and fruit plant.
Because the mechanism that giant cells produces is similar in all easily infected floristics, so in fact, method of the present invention also is applicable to all plants that can be changed by switch process of the present invention.
Method of the present invention is applicable to threadworms, but is not to only limit to M.incognita, M.javanica, M.arenaria and M.hapla.
The gene of discerning from infection plant He select preferably loses the gene that mobility is expressed later on basically nematode.
The sequence that will express (being positioned at chimeric gene) of described promotor control comprise following one or more:
1. can make the encoding sequence of the molecule of giant cells and/or excessive growth cell necrosis.
2. make the encoding sequence of the molecule of root knot nematode necrosis.
3. the encoding sequence that has any amount of enzyme that reduces the plant metabolism action activity.
4. infect the antisense strand of locus specificity gene.
5. the antisense strand of critical encoding sequence in the metabolic enzyme of vegetable cell.
If because at other site expressing gene, the expression product of the chimeric gene that this site produces has disadvantageous effect to the plant of conversion, is only at the gene of the cell expressing of giant cells and/or the overgrowth followed so generally be necessary to guarantee the gene of selecting.
As described in above-mentioned 1-5 point, one of advantage of the present invention is to give plant to the root knot nematode resistance, does not produce the product that nematicide infects and need not make up.
To be introduced preferred implementation step of the present invention below:
Growth of tobacco plant and infection
With Fisons F1 mixed fertilizer, C319 tobacco seed is germinateed, in illuminance irradiation 16 hours under the condition of 4500-5000lux, unglazed according to 8 hours time, temperature is 20-25 ℃.After about 3 weeks, wash seedling gently to remove soil, change (every box 2 strains in the box over to tap water; Northrupking), under 25 ℃, among the Conviron, illuminance is following 16 hours of 5500lux, one week of regrowth under the following 8 hours condition of unglazed photograph.From box top root is promoted, tip of a root place supports with glass fiber paper (Whatman GF/A).Three age in days nematodes with a 10 a μ l(50 nematode) (M.javanica) be released to the tip of a root, place the top so that the tip of a root is all coated with second GF/A paper again.Be to determine infectious cycle, infect and removed GF/A paper and freezing in liquid nitrogen immediately in back 24 hours, infect and prescinded root knot (staying healthy root and organization of root tips) in back 3 days, can collect the root tissue that about 0.5-1g infects from 80 strains inoculation plant.
The nematode of root is infected in dyeing with range estimation
In order to determine infect dose, measure the nematode number that every tip of a root infects.Collection is infected the root of the plant after 3 days and be dipped in 90 seconds in the cotton orchid that contains lactophenol 0.1% under 95 ℃.Then water carries out the flushing second time again, under room temperature (RT), this root is placed lactophenol 3-4 days with bleach.Then with the observation by light microscope nematode of dyeing.
Isolation of RNA from healthy and the root tissue that infects
In the pestle of cooled with liquid nitrogen and mortar with the root tissue pulverization, shift it in and add hot phenol extraction damping fluid (50% phenol of 300 μ l by the amount of every part of 100mg approximately with similar approach refrigerative Eppendorf pipe, 50% extraction damping fluid: 0.1M lithium chloride, 0.1M Tris-HCl, PH8.0(RT), 10mM EDTA is 1%SDS) and in 80 ℃ of following incubations 5 minutes.Add the equal-volume chloroform, under 4 ℃, little centrifugal homogenate 15 minutes.Water is with 600 μ l phenol/chloroform extractions and undertaken little centrifugal by as above condition.And then remove water, and under 4 ℃, with isopyknic lithium chloride RNA is precipitated and to spend the night, under the RT condition, carry out little centrifugally so that this precipitation is carried out granulation, and use 70% washing with alcohol.With gained pill lyophilized, resuspending is measured with spectrophotometer in the water that DEPC handles.Measure the amount of RNA with denaturing gel electrophoresis.(people such as Shirzadegan, change method in 1991).
The substrate clone of the special cDNAs that infects
By the explanation of manufacturers, with the few dT Dynabeads of magnetization from total amount be 200 μ g health with the C319 root tissue RNA sample that infects separate Poly(A)
+RNA(mRNA).Connecting health tissues Poly(A)
+Segmental Dynabead goes up original position and finishes article one cDNA.This cDNA drives DNA(Diver DNA).Infect the Poly(A of tissue in connection)
+Segmental Dynabead goes up synthetic first and second cDNA of original position.This cDNA is a target DNA.All the cDNA reaction is all used Pharmacid ' s cDNA synthetic agent box and is undertaken by the explanation of manufacturers.Three kinds of oligonucleotide SUB21(5 ' CTCTTGCTTGAATTCGGACTA3 '), SUB25(5 ' TAGTCCGAATTCAAGCAAGAGCACA3 ') (this sequence is selected from Duguid ﹠amp; Dinauer, 1990) and LDT15(5 ' GACAGAAGCGGATCCd(T) 15
3') people such as (, 1991) O ' Reilly all be with polynucleotide activating enzyme T according to people's such as Maniatis (nineteen eighty-two) method
4Activated.Press King ﹠amp then; Blakesley(1986) method of Pi Luing is with SUB21 and SUB25 annealing, at T
4Dna ligase is participated in down, is connected to form connector with target DNA.Thereafter with the pearl of carrying of this target of TE thorough washing, and in 95 ℃ down with 5XSSC wash-out second cDNA.
The RNA that will be connected on the Dynabead that drives DNA with heating means removes, and under 55 ℃, among the 5XSSC, drives DNA with the target DNA hybridization with this wash-out in 5 hours for this.Under room temperature, follow target DNA that the explanation of manufacturer will not hybridize and separate and remove from connecting the pearl that drives DNA.In 95 ℃ under, adopt similar way the target DNA of hybridization from connect the pearl separation that drive DNA removed thereafter.Be added back to the target DNA of RT wash-out among the said driving DNA then and repeat and hybridize, no longer surpass the not amount of hybridization for the amount that target drives DNA until hybridization.The concentration of DNA is measured by the explanation of the manufacturers DNADipstick with INvitrogen.
The final thing through wash-out of several equal portions is used for PCR to be amplified people such as (, nineteen ninety) Eckert to be used for the clone with generation is the bifilar cDNA of plasmid vector.Press the condition that people such as Frohman (1988) introduce, can obtain the amplification of target DNA with primer SUB21 and LDT15 and Hybaid Thermal Cycler.Press King ﹠amp; Blakesley(1986) method of Jie Shaoing is connected on the pBluescript carrier of Smal digestion the PCR product.
Utilize the routine library of reverse northern Analysis and Screening substrate
Use Gussow ﹠amp; Clackson(1989) method of Jie Shaoing is discerned recombinant chou with clone PCR.Press the makers' introduction of film, the three parts of traces of inset that amplify on Pall Biodyne film.Under same temperature and buffering solution, carry out prehybridization and hybridization.This temperature is that 42 ℃ and damping fluid are 5XSSPE, 0.05%BLOTTO, 50% methane amide.Again it is hybridized respectively for healthy and infect the cDNA probe (stating as follows) of tissue and comprise the probe of target DNA of the amplification of final substrate.Only select the cDNA probe that infects is shown hybridization signal or the probe of substrate is shown hybridization signal but the clone that the cDNA probe do not shown hybridization signal, do further to analyze.
Produce the cDNA probe
In order to obtain the highly single-minded active probe that has to the difference screening, " cold " synthesizes cDNA on whole RNA, and with few mark synthetic product carried out mark.Under 37 ℃, earlier handle healthy and infect the whole RNA samples of 10 μ g 15 minutes of tissue with 2.5 DNase1 of unit enzymes.In under 95 ℃ the DNase enzyme denaturation being synthesized cDNA(medicinal standard draft again after 10 minutes).In 0.4M sodium hydroxide exist and room temperature under, remove RNA with 10 fens clock times, and this DNA is with thin Spun Sephacry1400HR column purification.The productive rate of cDNA and concentration are measured (Invitrogen) with DNA Dipsticks.With this cDNA product being carried out mark (C.35ng/ probe) as the few mark of medicinal standard draft.
The Northern blotting
In order to measure the expression-form of cDNAs in different tissues of from the reverse northern analysis, selecting, no matter be whole or Poly(A at healthy or the root that infects, stem, Ye Hehua)+Northern of RNA analyzes all and clones as probe with this.All RNA trace per pass comprise 25 μ g RNA, and Poly(A)+the trace per pass contains RNA0.5-1 μ g.By method as described in people such as Fourney (1988), RNA is carried out electrophoresis and carry out trace on Pall Biodyne B film with formaldehyde gel.Mark and hybridization probe are trace as stated above.
The Southern blotting
In order to determine that this cDNAs derives from plant or nematode, press Gawel ﹠amp; Jarret(1991) method of Jie Shaoing prepares the DNA of C319 and M.javanica.Per pass contains the DNA of 10 μ g EcoR I and the digestion of Hind III in the Southern trace of preparation.This trace is hybridized to the probe of few mark by as above method.
In-situ hybridization
In order to determine that valuable cDNAs infecting the place that express in the site, carries out in-situ hybridization, press the method (1991) of Jackson, be embedded in the wax with tissue healthy root that infect, section and hybridization are probe.
Separating mRNA s 5 ' terminal
Determine to separate again after valuable RNAs 5 ' terminal their promoter sequence.Press the method that people such as Frohman (1988) introduce, use 5 ' RACE can realize this purpose.
Separate promoter region
The promoter region that separates required gene with the PCR method that is referred to as the carrier connection.By King ﹠amp in 1986; The method that Blackesky introduces, under the room temperature with the C319 genome DNA sample (100ng) of digestion with restriction enzyme with 4 hours (general used enzyme is EcoR I, BamH I, Hind III, BGl II, Xho I, Cla I, Sal I, Kpn I, Pst I and Sst I) of pBluescript sample (100ng) connection with the affine end of generation of digestion with restriction enzyme.PCR be use such as put in order-40 carrier primer or herewith the terminal complementary carrier of mRNA5 ' primer on linker, connect.Then this PCR product is cloned and sequencing.If necessary, in order to ensure the control sequence of separating promotor, use with promoter fragment 5 ' new primer of terminal complementary and repeat this method.
The construct of the chimeric gene in the binary plant conversion carrier
Isolating promotor by 5 ' terminal the connection, its sequence is a kind of sequence in the above-mentioned 1.-5. class, as itself being the antisense strand (as the 4th class) of this gene or barnase gene people such as (, 1972) Hartley (the 1st or the 3rd class).These are structures (Bevan, 1984) of binary vector.
The plant that producer gene changes
Can produce the plant that changes as tobacco one genoid with leaf-connecting disk method between the standard agricultural bacterium of people such as Horsch disclosure (1985), thereby the plant of anti-root knot nematode is provided.Plant seed or other propaguluies that the inventive method is produced can store use.
Such just as the skilled personnel to understand, for the plant of some class, except adopting above-mentioned agriculture bacterium indirect method, can also change plant with additive method, this is suitable or necessary.
One skilled in the art also know, in case root knot nematode is infected plant, the plant that produces according to the inventive method will no longer suffer to infect like this disadvantageous effect that is caused and also improve the fecundity of anti-root knot nematode simultaneously, root knot nematode number in the soil on this plant growth ground significantly reduces, and economic benefit is improved greatly.
Give plant all is suitable for for subjecting to monocotyledons, dicotyledons, herbaceous plant and xylophyta that root knot nematode infects the method for root knot nematode resistance according to the present invention.
Reference:
BEVAN，M.（1984）Nucleic Acids Research 12（22）：8711-8721.
DUGUID，J.R.& DINAUER，M.C.（1990）Nucleic Acids Research 18（9）：2789-2792.
ECKERT，K.A.& KUNKEL，T.A.（1990）Nucleic Acids Research 18（13）：3737-3744.
FOURNEY，R.M.，MIYAKOSHI，J.，DAY III，R.S.& PATERSON，M.C.（1988）Focus 10（1）：5-7.
FROHMAN，M.A.，DUSH，M.K.& MARTIN，G.R.（1988）Proceedings of the National Academy of Sciences USA 85：8998-9002.
GAWEL，N.J.& JARRET，R.L.（1991）.Plant Molecular Biology Reporter 9（3）：262-266.
GUSSOW，D.，& CLACKSON，T.（1989）.Nucleic Acids Research 17：4000.
GURR，S.J.，McPHERSON，M.J.，SCOLLAN，S.，ATKINSON，H.J.& BOWLES，D.J.（1991） Gene Expression in Nematode-Infected Plant Roots，Mol.Gen.Genet，226：361-366.
HARTLEY，R.W.，ROGERSON，D.L.，& SMEATON，J.R.（1972）Preparative Biochemistry 2：243-250.
HORSCH，R.B.，FRY，J.E.，HOFFMANN，N.L.，EICHHOLTZ，D.，ROGERS，S.G.& FRALEY，R.T.，（1985）Science 227：1229-1231.
JACKSON，D.（1991）.Molecular Plant Pathology：A Practical Approach.IRL Press，Oxford.
KING，P.V.& BLAKESLEY，R.W.（1986）.Focus 8（1）：1-3.
MANIATIS，T.，FRITSCH，E.F.& SAMBROOK，J.（1982）Molecular Cloning：A Laboratory Manual.NY.Cold Spring Harbour Laboratory.
O′REILLY，D.，THOMAS，C.J.R.& COUTTS，R.H.A.（1991）Journal of General Virology 72：1-7.
SHIRZADEGAN，M.CHRISTIE，P.& SEEMANN，J.R.（1991）Nucleic Acids Rescarch 19（21）：6055.
Claims (14)
1, a kind of method of producing the root knot nematode resistance plant, wherein, to the plant of being infected by root knot nematode, discerned the gene of the excessive growth cell that is expressed in plant root knot giant cells and/or follows, the promotor of described gene and encoding sequence merge so that the chimeric gene of coding molecule to be provided, this gene is harmful to one or more 1. root knot giant cellss, 2. root knot excessive growth cell and 3. root knot nematodes, and further described chimeric gene is transferred in the plant and gone.
2, according to the process of claim 1 wherein that described molecule is the molecule that can cause giant cells and/or excessive growth cell necrosis effectively.
3, according to the process of claim 1 wherein that described molecule is the molecule that can cause the root knot nematode necrosis effectively.
4, according to the process of claim 1 wherein that described molecule is to giving the molecule that the vegetable cell metabolism has enzymic activity.
5, according to the process of claim 1 wherein that described molecule is the RNA antisense strand that infects the described gene of plant identification from described.
6, according to the process of claim 1 wherein that described molecule is the RNA antisense strand of critical encoding gene in vegetable cell metabolism enzyme.
7, the root knot nematode resistance plant that obtains according to the method for above-mentioned each claim.
8, a kind of plant according to claim 7, wherein said plant is by the greengrocery plant, leguminous plant, fruit plant, one group of plant that nuts plant and cellulose family plant are formed.
9, a kind of plant according to claim 7, wherein said plant is a tobacco.
10, a kind of plant according to Claim 8, wherein said plant is a tomato.
11, a kind of plant according to Claim 8, wherein said plant Radix Dauci Sativae.
12, a kind of plant according to Claim 8, wherein said plant is a cotton.
13, a kind of plant propagulum that is plant according to claim 10.
14, a kind of chimeric gene that comprises encoding sequence, the molecule of its coding is harmful to one or more 1. root knot giant cellss, 2. root knot excessive growth cell and 3. root knot nematodes, described gene also comprises can operate the described promoter sequence that causes that described encoding sequence is expressed in root knot giant cells and/or root knot excessive growth cell.
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| GB9205474.1 | 1992-03-13 | ||
| GB929205474A GB9205474D0 (en) | 1992-03-13 | 1992-03-13 | Root knot nematode resistance |
Publications (2)
| Publication Number | Publication Date |
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| CN1077990A true CN1077990A (en) | 1993-11-03 |
| CN1080302C CN1080302C (en) | 2002-03-06 |
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ID=10712053
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN93104410A Expired - Lifetime CN1080302C (en) | 1992-03-13 | 1993-03-13 | Root knot nematode resistance |
Country Status (10)
| Country | Link |
|---|---|
| CN (1) | CN1080302C (en) |
| CZ (1) | CZ289067B6 (en) |
| GB (1) | GB9205474D0 (en) |
| GE (1) | GEP20002245B (en) |
| MD (1) | MD1400C2 (en) |
| MY (1) | MY109599A (en) |
| NZ (1) | NZ267026A (en) |
| TJ (1) | TJ287B (en) |
| TR (1) | TR28954A (en) |
| WO (1) | WO1993018170A1 (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1317383C (en) * | 2005-03-16 | 2007-05-23 | 云南大学 | Cystic monacrosporium janus prepn with nematocide function and its preparing method and use |
| CN100372935C (en) * | 2005-10-17 | 2008-03-05 | 华中农业大学 | Cloning of gene against meloidogyne of capsicum and application thereof |
Families Citing this family (17)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| ZA927576B (en) * | 1991-10-04 | 1993-04-16 | Univ North Carolina State | Pathogen-resistant transgenic plants. |
| US6008436A (en) * | 1993-01-21 | 1999-12-28 | North Carolina State University | Nematode-resistant transgenic plants |
| US5612471A (en) * | 1994-05-25 | 1997-03-18 | The Regents Of The University Of California | Nematode-induced genes in tomato |
| WO1997046692A1 (en) * | 1996-06-04 | 1997-12-11 | Mogen International N.V. | Nematode-inducible plant gene promoter |
| US6262344B1 (en) | 1995-06-13 | 2001-07-17 | Syngenta Mogen B.V. | Nematode-inducible plant gene promoter |
| EP1493817B1 (en) * | 1996-08-09 | 2010-12-29 | Keygene N.V. | Resistance against plant pests |
| EP0823481A1 (en) * | 1996-08-09 | 1998-02-11 | Keygene N.V. | Resistance against nematodes |
| US6392119B1 (en) | 1997-01-24 | 2002-05-21 | Dna Plant Technology Corporation | Two component plant cell lethality methods and compositions |
| GB0025225D0 (en) | 2000-10-14 | 2000-11-29 | Cambridge Advanced Tech | Plant cell death system |
| RS55986B1 (en) | 2010-01-22 | 2017-09-29 | Bayer Ip Gmbh | Acaricides and/or insecticidal agent combinations |
| WO2012059497A1 (en) | 2010-11-02 | 2012-05-10 | Bayer Cropscience Ag | N-hetarylmethyl pyrazolylcarboxamides |
| US20130289077A1 (en) | 2010-12-29 | 2013-10-31 | Juergen Benting | Fungicide hydroximoyl-tetrazole derivatives |
| CN103717076B (en) | 2011-08-10 | 2016-04-13 | 拜耳知识产权股份有限公司 | Active compound combinations containing specific tetramic acid derivatives |
| AR093909A1 (en) | 2012-12-12 | 2015-06-24 | Bayer Cropscience Ag | USE OF ACTIVE INGREDIENTS TO CONTROL NEMATODES IN CULTURES RESISTANT TO NEMATODES |
| MX360582B (en) | 2012-12-13 | 2018-11-07 | Inst De Ecologia A C Star | Biocontrol of phyto-parasitic nematodes using paecilomyces. |
| MD719Z (en) * | 2013-06-11 | 2014-08-31 | Институт Зоологии Академии Наук Молдовы | Method for treating potatoes against nematode Ditylencus destructor |
| CA2994588A1 (en) | 2015-08-07 | 2017-02-16 | Bayer Cropscience Nv | Root-preferential and stress inducible promoter and uses thereof |
Family Cites Families (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| ES2165345T3 (en) * | 1987-07-10 | 2002-03-16 | Syngenta Participations Ag | Induced viral resistance in plants. |
| CN1033645A (en) * | 1988-10-22 | 1989-07-05 | 中国科学院上海植物生理研究所 | The gene engineering method of controlling plant virus disease |
| JPH06503470A (en) * | 1990-09-10 | 1994-04-21 | アドバーンスト テクノロジーズ （ケンブリッジ） リミテッド | How to control nematodes parasitic on plants |
| WO1992021757A1 (en) * | 1991-05-30 | 1992-12-10 | Plant Genetic Systems, N.V. | Nematode-responsive plant promoters |
| ZA927576B (en) * | 1991-10-04 | 1993-04-16 | Univ North Carolina State | Pathogen-resistant transgenic plants. |
| HU218897B (en) * | 1991-11-20 | 2000-12-28 | Mogen International N. V. | Plants with reduced susceptibility to plant-parasitic nematodes and method for producing them |
-
1992
- 1992-03-13 GB GB929205474A patent/GB9205474D0/en active Pending
-
1993
- 1993-03-09 MY MYPI93000414A patent/MY109599A/en unknown
- 1993-03-11 GE GEAP19932430A patent/GEP20002245B/en unknown
- 1993-03-11 CZ CZ19942103A patent/CZ289067B6/en not_active IP Right Cessation
- 1993-03-11 MD MD96-0266A patent/MD1400C2/en unknown
- 1993-03-11 WO PCT/GB1993/000514 patent/WO1993018170A1/en active IP Right Grant
- 1993-03-11 NZ NZ267026A patent/NZ267026A/en not_active IP Right Cessation
- 1993-03-11 TJ TJ96000329A patent/TJ287B/en unknown
- 1993-03-12 TR TR00201/93A patent/TR28954A/en unknown
- 1993-03-13 CN CN93104410A patent/CN1080302C/en not_active Expired - Lifetime
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1317383C (en) * | 2005-03-16 | 2007-05-23 | 云南大学 | Cystic monacrosporium janus prepn with nematocide function and its preparing method and use |
| CN100372935C (en) * | 2005-10-17 | 2008-03-05 | 华中农业大学 | Cloning of gene against meloidogyne of capsicum and application thereof |
Also Published As
| Publication number | Publication date |
|---|---|
| TJ287B (en) | 2000-12-13 |
| MD960266A (en) | 1998-01-31 |
| MY109599A (en) | 1997-03-31 |
| CZ289067B6 (en) | 2001-10-17 |
| TR28954A (en) | 1997-08-04 |
| CN1080302C (en) | 2002-03-06 |
| NZ267026A (en) | 1995-08-28 |
| GEP20002245B (en) | 2000-09-25 |
| WO1993018170A1 (en) | 1993-09-16 |
| MD1400C2 (en) | 2000-10-31 |
| GB9205474D0 (en) | 1992-04-29 |
| MD1400B2 (en) | 2000-01-31 |
| CZ210394A3 (en) | 1997-05-14 |
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