CN107779441B - 一种禽流感抗原的浓缩纯化方法、禽流感抗原 - Google Patents
一种禽流感抗原的浓缩纯化方法、禽流感抗原 Download PDFInfo
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- CN107779441B CN107779441B CN201711105661.0A CN201711105661A CN107779441B CN 107779441 B CN107779441 B CN 107779441B CN 201711105661 A CN201711105661 A CN 201711105661A CN 107779441 B CN107779441 B CN 107779441B
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- avian influenza
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- influenza antigen
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- 239000000427 antigen Substances 0.000 title claims abstract description 45
- 102000036639 antigens Human genes 0.000 title claims abstract description 45
- 108091007433 antigens Proteins 0.000 title claims abstract description 45
- 208000002979 Influenza in Birds Diseases 0.000 title claims abstract description 44
- 206010064097 avian influenza Diseases 0.000 title claims abstract description 44
- 238000000034 method Methods 0.000 title claims abstract description 31
- 238000000746 purification Methods 0.000 title abstract description 22
- 239000007788 liquid Substances 0.000 claims abstract description 108
- 238000010992 reflux Methods 0.000 claims abstract description 56
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 claims abstract description 26
- 241000712461 unidentified influenza virus Species 0.000 claims abstract description 24
- 239000012528 membrane Substances 0.000 claims abstract description 23
- 239000008118 PEG 6000 Substances 0.000 claims abstract description 15
- 229920002584 Polyethylene Glycol 6000 Polymers 0.000 claims abstract description 15
- MTHSVFCYNBDYFN-UHFFFAOYSA-N diethylene glycol Chemical compound OCCOCCO MTHSVFCYNBDYFN-UHFFFAOYSA-N 0.000 claims abstract description 15
- 239000011780 sodium chloride Substances 0.000 claims abstract description 11
- 239000002244 precipitate Substances 0.000 claims abstract description 10
- 238000002156 mixing Methods 0.000 claims abstract description 8
- 238000001914 filtration Methods 0.000 claims description 15
- 239000011148 porous material Substances 0.000 claims description 10
- 239000012510 hollow fiber Substances 0.000 claims description 9
- 238000001471 micro-filtration Methods 0.000 claims description 8
- 238000005119 centrifugation Methods 0.000 claims description 4
- 238000002360 preparation method Methods 0.000 claims description 2
- 150000001875 compounds Chemical class 0.000 claims 1
- 241000700605 Viruses Species 0.000 abstract description 47
- 239000002158 endotoxin Substances 0.000 abstract description 9
- 102000004169 proteins and genes Human genes 0.000 abstract description 9
- 108090000623 proteins and genes Proteins 0.000 abstract description 9
- 239000000047 product Substances 0.000 abstract description 2
- 210000003837 chick embryo Anatomy 0.000 description 17
- 210000002257 embryonic structure Anatomy 0.000 description 15
- 239000012530 fluid Substances 0.000 description 15
- 239000012535 impurity Substances 0.000 description 11
- 238000003306 harvesting Methods 0.000 description 7
- 238000009295 crossflow filtration Methods 0.000 description 6
- 230000035931 haemagglutination Effects 0.000 description 6
- 230000023555 blood coagulation Effects 0.000 description 5
- 238000011534 incubation Methods 0.000 description 5
- 238000000926 separation method Methods 0.000 description 5
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 4
- 241000287828 Gallus gallus Species 0.000 description 4
- 238000001514 detection method Methods 0.000 description 4
- 238000007865 diluting Methods 0.000 description 4
- 210000003743 erythrocyte Anatomy 0.000 description 4
- 239000002245 particle Substances 0.000 description 4
- 238000003756 stirring Methods 0.000 description 4
- 239000002202 Polyethylene glycol Substances 0.000 description 3
- 230000015271 coagulation Effects 0.000 description 3
- 238000005345 coagulation Methods 0.000 description 3
- 239000011362 coarse particle Substances 0.000 description 3
- 238000002474 experimental method Methods 0.000 description 3
- 239000012467 final product Substances 0.000 description 3
- 238000004519 manufacturing process Methods 0.000 description 3
- 238000005374 membrane filtration Methods 0.000 description 3
- 229920001223 polyethylene glycol Polymers 0.000 description 3
- 238000004659 sterilization and disinfection Methods 0.000 description 3
- 239000006228 supernatant Substances 0.000 description 3
- 238000000108 ultra-filtration Methods 0.000 description 3
- 239000005723 virus inoculator Substances 0.000 description 3
- 208000035473 Communicable disease Diseases 0.000 description 2
- 208000005342 Porcine Reproductive and Respiratory Syndrome Diseases 0.000 description 2
- 210000004027 cell Anatomy 0.000 description 2
- 239000012141 concentrate Substances 0.000 description 2
- 244000144992 flock Species 0.000 description 2
- 230000001900 immune effect Effects 0.000 description 2
- 229940031551 inactivated vaccine Drugs 0.000 description 2
- 230000000415 inactivating effect Effects 0.000 description 2
- 208000015181 infectious disease Diseases 0.000 description 2
- 238000011081 inoculation Methods 0.000 description 2
- 210000001161 mammalian embryo Anatomy 0.000 description 2
- 230000001717 pathogenic effect Effects 0.000 description 2
- 229960005486 vaccine Drugs 0.000 description 2
- 241000271566 Aves Species 0.000 description 1
- 241001473386 H9N2 subtype Species 0.000 description 1
- 241000712431 Influenza A virus Species 0.000 description 1
- 241000239218 Limulus Species 0.000 description 1
- 241000712464 Orthomyxoviridae Species 0.000 description 1
- 206010035148 Plague Diseases 0.000 description 1
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 description 1
- 229930006000 Sucrose Natural products 0.000 description 1
- 241000607479 Yersinia pestis Species 0.000 description 1
- 230000001154 acute effect Effects 0.000 description 1
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- 125000005842 heteroatom Chemical group 0.000 description 1
- 238000005286 illumination Methods 0.000 description 1
- 230000008105 immune reaction Effects 0.000 description 1
- 230000003053 immunization Effects 0.000 description 1
- 229960003971 influenza vaccine Drugs 0.000 description 1
- 238000011835 investigation Methods 0.000 description 1
- 150000002632 lipids Chemical class 0.000 description 1
- 210000001589 microsome Anatomy 0.000 description 1
- 230000017448 oviposition Effects 0.000 description 1
- 239000008188 pellet Substances 0.000 description 1
- 244000144977 poultry Species 0.000 description 1
- 230000001376 precipitating effect Effects 0.000 description 1
- 230000002265 prevention Effects 0.000 description 1
- 208000023504 respiratory system disease Diseases 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 239000005720 sucrose Substances 0.000 description 1
- 238000004448 titration Methods 0.000 description 1
- 230000001018 virulence Effects 0.000 description 1
Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N7/00—Viruses; Bacteriophages; Compositions thereof; Preparation or purification thereof
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2760/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssRNA viruses negative-sense
- C12N2760/00011—Details
- C12N2760/16011—Orthomyxoviridae
- C12N2760/16111—Influenzavirus A, i.e. influenza A virus
- C12N2760/16121—Viruses as such, e.g. new isolates, mutants or their genomic sequences
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2760/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssRNA viruses negative-sense
- C12N2760/00011—Details
- C12N2760/16011—Orthomyxoviridae
- C12N2760/16111—Influenzavirus A, i.e. influenza A virus
- C12N2760/16151—Methods of production or purification of viral material
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- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Wood Science & Technology (AREA)
- Virology (AREA)
- Biotechnology (AREA)
- Microbiology (AREA)
- Biomedical Technology (AREA)
- Medicinal Chemistry (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Immunology (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Peptides Or Proteins (AREA)
- Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
Abstract
Description
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CN201711105661.0A CN107779441B (zh) | 2017-11-10 | 2017-11-10 | 一种禽流感抗原的浓缩纯化方法、禽流感抗原 |
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CN107779441A CN107779441A (zh) | 2018-03-09 |
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CN108676781A (zh) * | 2018-05-18 | 2018-10-19 | 山东信得动物疫苗有限公司 | 一种重组禽流感病毒的工业化纯化方法 |
Citations (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN102600468A (zh) * | 2012-03-01 | 2012-07-25 | 浙江天元生物药业有限公司 | 一种含mf59佐剂禽流感病毒裂解疫苗的制备工艺 |
CN103601793A (zh) * | 2013-10-23 | 2014-02-26 | 乾元浩生物股份有限公司 | 一种禽用疫苗抗原纯化的方法 |
CN105214082A (zh) * | 2014-07-04 | 2016-01-06 | 乾元浩生物股份有限公司 | 鸡新城疫、禽流感二联灭活疫苗及其制备方法 |
CN105349499A (zh) * | 2015-11-06 | 2016-02-24 | 张澍 | 一种禽流感全病毒颗粒标记疫苗的制备方法及其产品和用途 |
CN106540249A (zh) * | 2015-09-19 | 2017-03-29 | 广东永顺生物制药股份有限公司 | 一种禽流感(h5n1)或猪繁殖与呼吸综合征(prrs)病毒疫苗的抗原浓缩纯化方法 |
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- 2017-11-10 CN CN201711105661.0A patent/CN107779441B/zh active Active
Patent Citations (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN102600468A (zh) * | 2012-03-01 | 2012-07-25 | 浙江天元生物药业有限公司 | 一种含mf59佐剂禽流感病毒裂解疫苗的制备工艺 |
CN103601793A (zh) * | 2013-10-23 | 2014-02-26 | 乾元浩生物股份有限公司 | 一种禽用疫苗抗原纯化的方法 |
CN105214082A (zh) * | 2014-07-04 | 2016-01-06 | 乾元浩生物股份有限公司 | 鸡新城疫、禽流感二联灭活疫苗及其制备方法 |
CN106540249A (zh) * | 2015-09-19 | 2017-03-29 | 广东永顺生物制药股份有限公司 | 一种禽流感(h5n1)或猪繁殖与呼吸综合征(prrs)病毒疫苗的抗原浓缩纯化方法 |
CN105349499A (zh) * | 2015-11-06 | 2016-02-24 | 张澍 | 一种禽流感全病毒颗粒标记疫苗的制备方法及其产品和用途 |
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Inventor after: Xu Jin Inventor after: Luo Aifang Inventor after: Ding Shaocheng Inventor after: Guo Xiaoqing Inventor after: Xie Meimei Inventor after: Li Houwei Inventor after: Fan Huili Inventor before: Xie Meimei Inventor before: Xu Jin Inventor before: Li Houwei |
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PE01 | Entry into force of the registration of the contract for pledge of patent right |
Denomination of invention: A concentration and purification method for avian influenza antigen, avian influenza antigen Granted publication date: 20210416 Pledgee: Henan Xinzheng Rural Commercial Bank Co.,Ltd. Pledgor: HENAN HOUYI BIOENGINEERING CO.,LTD. Registration number: Y2024980015199 |
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