CN107771859A - A kind of containing fluopyram and the nematicide of bacillus subtilis - Google Patents
A kind of containing fluopyram and the nematicide of bacillus subtilis Download PDFInfo
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- CN107771859A CN107771859A CN201711240358.1A CN201711240358A CN107771859A CN 107771859 A CN107771859 A CN 107771859A CN 201711240358 A CN201711240358 A CN 201711240358A CN 107771859 A CN107771859 A CN 107771859A
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- Prior art keywords
- bacillus subtilis
- fluopyram
- nematicide
- root
- knot nematode
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/34—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one nitrogen atom as the only ring hetero atom
- A01N43/40—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one nitrogen atom as the only ring hetero atom six-membered rings
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- Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Health & Medical Sciences (AREA)
- Zoology (AREA)
- Dentistry (AREA)
- Agronomy & Crop Science (AREA)
- Environmental Sciences (AREA)
- Pest Control & Pesticides (AREA)
- Plant Pathology (AREA)
- Wood Science & Technology (AREA)
- Microbiology (AREA)
- Virology (AREA)
- Biotechnology (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The present invention relates to a kind of containing fluopyram and the nematicide of bacillus subtilis, active ingredient be made up of fluopyram and bacillus subtilis set content as 1,000,000,000,000 gemma/gram bacillus subtilis active compound be 100% active compound, the weight ratio of fluopyram and bacillus subtilis is 20 80:1.Synergistic of the present invention, can effectively preventing root knot nematode disease, advantageously reduce the usage amount of drug cost and chemical pesticide, delay the resistance of root-knot nematode, extend the life cycle of fluopyram.
Description
Technical field
The present invention relates to agricultural technology field, nematode is killed more particularly to a kind of containing fluopyram and bacillus subtilis
Agent.
Background technology
Plant nematode mainly includes：Meloidogyne (Meloidogyne), kidney shape turbatrix
(Rotylenchulus), cyst roundworm (Heterodera (Heterodera)), root spot nematode (Pratylenchus
(Pratykenchus)), root-knot nematode occurs universal in China, and harm is serious.Plant can be greatly decreased in the direct feeding of nematode
To nutrition and the absorption of moisture, nematode Plant species eaten causes wound, returns phytopathogen invasion and provides convenience.Nematode at present
Preventing and treating alternative is few, agriculturally prevents and treats nematode and concentrates on invasion and attack of the prevention nematode to plant substantially.With eelworm harm
Getting worse, it is badly in need of developing new, efficient, less toxic nematicide.
Fluopyram, English name：Fluopyram, it is the new pyridine base ethyl benzamide of Beyer Co., Ltd's exploitation
Series bactericidal agent, nematicide, act on mitochondrial respiratory chain, suppress the activity of succinate dehydrogenase (complexⅱ) so as to block
Electron transmission, cause the energy requirement that body tissue can not be provided, and then kill controlling object or suppress it and grow.
Bacillus subtilis, English name：Bacillus subtilis, be a kind of microbial bactericide, by effectively into
Divide the antibacterial material and site competition, the mode of action of induction of resistance of producing bacillus subtilis life, kill and control pathogen, from
And reach the purpose of controlling disease.
It is preventing and treating resistance disease pest using the farm chemical ingredients mixture of different mechanism of action in the real process of agricultural production
Harmful common methods.Screened by formulation of pesticide, show that mixture has Synergistic in certain ratio range between heterogeneity
Effect, it is possible to increase actual prevention effect, be the important means for administering pest and disease damage resistance.
At present, it is also no related as the control of nematode medicament of active ingredient to bacillus subtilis using fluopyram
Report.
The content of the invention
It is an object of the invention to provide a kind of containing fluopyram and the nematicide of bacillus subtilis, to improve root knot
The prevention effect of nematode, delay resistance of the root-knot nematode to single medicament, reduce cost accounting, extend the life of fluopyram
Cycle.
The technical solution used in the present invention is:A kind of containing fluopyram and the nematicide of bacillus subtilis, have
Effect composition be made up of fluopyram and bacillus subtilis, set content as 1,000,000,000,000 gemma/gram bacillus subtilis original
Medicine is 100% active compound, and the weight ratio of fluopyram and bacillus subtilis is 20-80:1, it is preferred that fluopyram and withered
The weight ratio of careless bacillus is 50:1.
Described nematicide, content of the fluopyram in nematicide are 2.0%-8.0%, bacillus subtilis
Content in nematicide for 500,000,000 spores/gram~2,000,000,000 spores/gram, i.e. content of the bacillus subtilis in bactericide
0.05-0.2%.
Described nematicide is used in preventing and treating Cereal, rice, wheat, fruit tree, cotton, vegetables, soybean and peanut etc.
The nematodiasis of oil crops, flowers etc., as wheat root-knot nematode and cyst roundworm, citrus root-knot nematode, cucumber root-knot nematode, kind
Eggplant root-knot nematode, capsicum root-knot nematode, soy bean cyst roundworm, peanut root-knot nematode etc..
Described nematicide is configured to spray or carry out soil or suspending agent, the water and milk of seed treatment after being available for dilution
Agent, microemulsion, wettable powder, water dispersible granules, it may be made as granule and directly apply.Preferred dosage form is granule.
Described nematicide be prepared into the adjuvant that suspending agent or granule add include solvent, filler, binding agent, point
Powder, wetting agent, thickener, sustained release agent, stabilizer or it is other be beneficial to active component in the formulation stablize and drug effect play
Material, all it is to be commonly used in agriculture field preparation or allow the various composition used.
The present invention has advantages below and notable technique effect：
(1) active ingredient bacillus subtilis of the invention and fluopyram Synergistic, are used with the reasonable scope,
The amount of application of each single dose can be reduced, advantageously reduces the usage amount of drug cost and chemical pesticide.
(2) active ingredient bacillus subtilis of the present invention is different with the mechanism of action of fluopyram, bacillus subtilis
The resistance of aggrieved diseased plant can also be improved, delays the resistance of root-knot nematode, extends the life cycle of fluopyram.
Embodiment
Technical scheme and beneficial effect are described further with reference to embodiments, of the present invention hundred
Ratio is divided to be weight percentage.
First, indoor joint toxicity measuring test
Embodiment 1：Fluopyram compounds the toxicity test to cucumber root-knot nematode with bacillus subtilis
Subjects：Cucumber root-knot nematode (Meloidogyne incognita)
Test method：Pot-culture method, the pieces of an egg sterile water wash of picking root-knot nematode, is placed in culture dish from cucumber root
On wet filter paper, 20ml sterilized waters are added, are hatched at 20 DEG C -25 DEG C, age consistent second instar larvae is obtained, is diluted to sterilized water
400/ml.Second instar larvae is inoculated with the leaf phase of cucumber two, using root injection method, takes 5ml nematodes inoculation liquid to inject rhizosphere soil
It is interior, 24h soil conservations 70%-80% humidity after inoculation.It is standby after cucumber inoculation root-knot nematode.
Mother liquor is made into after active ingredient is dissolved on request, then carries out pouring root with purified water gradient dilution to suitable concentration
Processing.Cucumber seedling is placed in phjytotron after chemicals treatment, then 25-30 DEG C, 12h alternation of light and darkness, relative humidity 35-
30d is cultivated under the conditions of 50%.Each to repeat the basin of random searching 4, each processing amounts to 16 basin cucumber of investigation.Grade scale is by China
People's republic's national standard《Pesticide field efficacy medicine test criterion (one)》, severity Scaling standard.
Diseased plant stage division is as follows：
0 grade：Root system is without insect gall；
1 grade：Root system has a small amount of small insect gall；
3 grades：2/3rds root systems are covered with small insect gall；
5 grades：Root system is covered with small insect gall and has secondary insect gall；
7 grades：Root system forms fibrous root group；
Computational methods：
Prevention effect is converted into niqueMin (y), liquor strength (μ g/ml) is converted into logarithm value (x), in terms of least square
Concentration EC in calculating virulence equation and suppressing50, according to the toxicity index and co-toxicity coefficient (CTC) of the abundant method calculating medicaments of Sun Yun.
Survey toxicity index (ATI)=(standard agent EC50/ reagent agent EC50)×100
A percentage composition+B medicament toxicity index in theoretical toxicity index (TTI)=A medicament toxicity index × mixture × mixed
B percentage composition in agent
Co-toxicity coefficient (CTC)=[mixture actual measurement toxicity index (ATI)/mixture theoretical toxicity index (TTI)] × 100
When CTC≤80, then composition shows as antagonism, and as 80 ＜ CTC ＜ 120, then composition shows as being added and made
With when CTC >=120, then composition shows as synergistic effect.
Result of the test is shown in Table 1.
The fluopyram of table 1 compounds the toxicity test to cucumber root-knot nematode with bacillus subtilis
The measurement result of indoor co-toxicity shows that fluopyram compares 1-20 with bacillus subtilis with weight:1 mixture
When, co-toxicity coefficient (CTC) has synergistic function to be all higher than 120.When fluopyram and the weight of bacillus subtilis
Than 8:When 1, CTC 188.9, synergistic effect is more notable, there is fine guiding value in field test.
2nd, embodiment is prepared
Embodiment 1：The CFU/ grams of bacillus subtilis granule of 5% fluopyram 1,000,000,000
Formula：Fluopyram 5.0%, bacillus subtilis 0.1% are weighed according to quantity, and sodium lignin sulfonate 3.2% (disperses
Agent), lauryl sodium sulfate 2.5% (wetting agent), polyvinyl alcohol 2.5% (binding agent), white carbon 10% (filler), diatomite
(filler) complements to 100%.Each composition is sufficiently mixed in the ratio of formula by more than, is crushed, and adds water to be sufficiently stirred again after soaking
Uniformly, then with screw extruder pelletizer it is granulated, is sieved after drying, produce the CFU/ grams of withered grass gemma of 5% fluopyram 1,000,000,000
Bacillus granule.
Embodiment application preventing and treating banana root-knot nematode, by the CFU/ grams of bacillus subtilis of 5% fluopyram 1,000,000,000
Granule dosage is handled for the ditch spread of 1.5g/ mus, and the prevention effect of 20 days and 50 days is respectively 88.6%, 85.4% after medicine.5% fluorine
Pyrrole bacterium acid amides granule, dosage are 2.5g/ mus, are in kind used, after medicine the preventive effect of 20 days and 50 days be respectively 72.3%,
67.2%；10000000000 CFU/ grams of bacillus subtilis granules, dosage 5.0kg/ mus, are in kind used, 20 days and 50 after medicine
It preventive effect is respectively 61.8%, 52.3%.
This explanation, fluopyram is with synergistic effect after bacillus subtilis compounding substantially to the preventive effect of banana root-knot nematode
Significantly better than single dose.
Embodiment 2：The CFU/ grams of bacillus subtilis granule of 2% fluopyram 500,000,000
Formula:Fluopyram 5.0%, bacillus subtilis 0.05%, naphthalenesulfonate formaldehyde condensation compound are weighed according to quantity
3.5% (dispersant), lauryl sodium sulfate 3.5% (wetting agent), polyvinyl alcohol 3.0% (binding agent), attapulgite 10%
(filler), diatomite (filler) complement to 100%.Each composition is well mixed in the ratio of formula by more than, through air-flow crushing into
Pulvis, add a certain amount of water mixing extruding and make material, the CFU/ grams of withered grass bud of 2% fluopyram 500,000,000 is obtained after drying screening
Spore bacillus granule.
The embodiment is applied to preventing and treating peanut root-knot nematode.The CFU/ grams of bacillus subtilis of 2% fluopyram 500,000,000
Granula, dosage are handled for the ditch spread of 2.0g/ mus, and the prevention effect of 20 days and 50 days is respectively 92.8%, 87.6% after medicine.2.5%
Fluopyram granule, dosage are 2.5KG/ mus, are in kind used, the preventive effect of 20 days and 50 days is respectively after medicine
76.2%th, 63.2%；10000000000 CFU/ grams of bacillus subtilis granules, dosage 4.0kg/ mus, are in kind used, after medicine
The preventive effect of 20 days and 50 days is respectively 61.9%, 52.6%.Illustrate that fluopyram is made with synergy after bacillus subtilis compounding
With obvious, single dose is significantly better than to the preventive effect of peanut root-knot nematode.
In summary, nematicide of the invention has obvious synergistic effect, can effectively control the danger of root-knot nematode
Evil, reduces the dosage of fluopyram, reduces cost, delay nematode resistance to develop, and it is online to be advantageous to extension fluopyram
Worm applies upper life cycle.
Claims (3)
1. a kind of containing fluopyram and the nematicide of bacillus subtilis, it is characterised in that active ingredient is by fluorine pyrrole bacterium acyl
Amine and bacillus subtilis composition, set content as 1,000,000,000,000 gemma/gram bacillus subtilis active compound be 100% active compound, fluorine
The weight of pyrrole bacterium acid amides and bacillus subtilis ratio is 20-80:1.
2. nematicide according to claim 1, it is characterised in that fluopyram and the weight of bacillus subtilis ratio
For 50:1.
3. nematicide according to claim 1 or 2, it is characterised in that content of the fluopyram in nematicide
For 2.0%-8.0%, content 0.05-0.2% of the bacillus subtilis in nematicide.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201711240358.1A CN107771859A (en) | 2017-11-30 | 2017-11-30 | A kind of containing fluopyram and the nematicide of bacillus subtilis |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201711240358.1A CN107771859A (en) | 2017-11-30 | 2017-11-30 | A kind of containing fluopyram and the nematicide of bacillus subtilis |
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| Publication Number | Publication Date |
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| CN107771859A true CN107771859A (en) | 2018-03-09 |
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Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103442575A (en) * | 2010-09-22 | 2013-12-11 | 拜耳知识产权有限责任公司 | Use of biological or chemical control agents for controlling insects and nematodes in resistant crops |
| CN104507314A (en) * | 2012-01-25 | 2015-04-08 | 拜耳知识产权有限责任公司 | Active compounds combination containing fluopyram bacillus and biologically control agent |
| CN104902753A (en) * | 2012-05-30 | 2015-09-09 | 拜耳农作物科学股份公司 | Compositions comprising a biological control agent and an insecticide |
| CN104957173A (en) * | 2010-12-01 | 2015-10-07 | 拜耳知识产权有限责任公司 | Active ingredient combinations comprising pyridylethylbenzamides and other active ingredients |
-
2017
- 2017-11-30 CN CN201711240358.1A patent/CN107771859A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103442575A (en) * | 2010-09-22 | 2013-12-11 | 拜耳知识产权有限责任公司 | Use of biological or chemical control agents for controlling insects and nematodes in resistant crops |
| CN104957173A (en) * | 2010-12-01 | 2015-10-07 | 拜耳知识产权有限责任公司 | Active ingredient combinations comprising pyridylethylbenzamides and other active ingredients |
| CN104507314A (en) * | 2012-01-25 | 2015-04-08 | 拜耳知识产权有限责任公司 | Active compounds combination containing fluopyram bacillus and biologically control agent |
| CN104902753A (en) * | 2012-05-30 | 2015-09-09 | 拜耳农作物科学股份公司 | Compositions comprising a biological control agent and an insecticide |
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