CN107771667A - A kind of method that seed germination rate is improved in gastrodia elata sexual propagation breeding process - Google Patents
A kind of method that seed germination rate is improved in gastrodia elata sexual propagation breeding process Download PDFInfo
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- CN107771667A CN107771667A CN201610772675.7A CN201610772675A CN107771667A CN 107771667 A CN107771667 A CN 107771667A CN 201610772675 A CN201610772675 A CN 201610772675A CN 107771667 A CN107771667 A CN 107771667A
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- rhizoma gastrodiae
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
- A01H1/02—Methods or apparatus for hybridisation; Artificial pollination ; Fertility
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01C—PLANTING; SOWING; FERTILISING
- A01C1/00—Apparatus, or methods of use thereof, for testing or treating seed, roots, or the like, prior to sowing or planting
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- Genetics & Genomics (AREA)
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- Developmental Biology & Embryology (AREA)
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- Micro-Organisms Or Cultivation Processes Thereof (AREA)
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Abstract
The present invention provides a kind of method that seed germination rate is improved in gastrodia elata sexual propagation breeding process, traditionally cultivates close ring bacterium bacterium material July last year；Traditionally cultivate wood sawdust culture medium Germination Strain polybag strain November last year；November last year selects the preservation under the conditions of natural temperature of no disease and pests harm rhizoma Gastrodiae；The first tenday period of a month January next year, by traditionally vernalization and the artificial pollination of the rhizoma Gastrodiae of preservation, harvesting late March rhizoma Gastrodiae modeling fruit；Polybag strain point is broken as the fritter of 2cm sizes；Rhizoma Gastrodiae capsule, which is broken into two with one's hands, takes kind to admix in fritter；It is put into the closing of breeding bag and stays airing hole, cultivates 4~7 days；Fine sand is admixed into the Germination Strain fungus block unpacking cultivated and loads breeding bag, opening, which is amassed wealth by heavy taxation, educates 20 days, and surface forms auburn mycoderm and can be used to field planting.Cultivating the Germination Strain fritter for being mixed with seeds of Gastrodia elata has the function that drought resisting and moisture-resistant, can reduce the withered and rotten loss of strain, improve seed germination rate.
Description
Technical field
The present invention relates to a kind of gastrodia elata sexual propagation breeding method, specifically a kind of gastrodia elata sexual propagation breeding process
The middle method for improving seed germination rate.
Background technology
The seed of rhizoma Gastrodiae is minimum, and such as powder, without endosperm and other nutrition, itself can not sprout seed, need Germination Strain to infect
Nutrition is provided afterwards for seeds of Gastrodia elata to sprout.Because seeds of Gastrodia elata is minimum, pole not storage endurance, at present all using with harvesting Gastrodia
Son is with mixing planting patterns of the Germination Strain with lower crop field.This planting patterns exist be mixed with seeds of Gastrodia elata Germination Strain it is easily withered and
Rotten phenomenon, cause gastrodia seed germination rate low.
The content of the invention
It is an object of the invention to solve above-mentioned problem, there is provided be improved in a kind of gastrodia elata sexual propagation breeding process
The method of seed germination rate, with the high effect of drought-enduring moisture-proof and germination rate.
The present invention includes traditionally vernalization and artificial pollination, and used technical scheme is：
Step 1, in upper in July, 1 traditionally cultivated close ring bacterium bacterium material；
Step 2, traditionally to cultivate in upper in November, 1 wood sawdust culture medium Germination Strain polybag strain stand-by；
Step 3, upper in November, 1 select 200~250g/ weight no disease and pests harm rhizoma Gastrodiae protected under the conditions of natural temperature
Hide；
Step 4, in the first tenday period of a month January next year, by traditionally vernalization and the artificial pollination of the rhizoma Gastrodiae of institute's preservation, harvesting late March
Rhizoma Gastrodiae modeling fruit will be split；
Step 5, step 2 stand-by polybag strain point broken it is stand-by for the Germination Strain fritter of 2cm sizes；
Step 6, step 4 will be split into rhizoma Gastrodiae capsule break into two with one's hands and take seeds of Gastrodia elata to admix in the stand-by Germination Strain fritter of step 5, admix
When, the seeds of Gastrodia elata of 15~20 rhizoma Gastrodiae capsules is admixed by every Kg modeling bacterium germination fritters, and allow seeds of Gastrodia elata to be uniformly distributed adhesion
In Germination Strain fritter surrounding；
Step 7, the above-mentioned Germination Strain fungus block mixed is put into close in breeding bag and stays airing hole, by upper and lower one group of counterpart two bag
It is placed in breeding chamber, is cultivated 4~7 days under 20~25 DEG C of dry environments；
Step 8, above-mentioned cultivate after surface forms the Germination Strain fungus block unpacking of dense white hypha admixed into fine sand, make strain block
Surface sticks the grains of sand；
Step 9, the Germination Strain fritter that will be stained with the grains of sand load breeding bag, and opening is put into breeding chamber and is put and amassed wealth by heavy taxation by every bag of interval 2cm
Educate 20 days, cover 2cm fine sand after putting on breeding bag, fine sand humidity is maintained at 40%~50% during cultivation, and humidity is too low
When, moisturizing of spraying water, form auburn mycoderm on Germination Strain fritter surface after 20 days and can be used to field planting.
In above-mentioned technical proposal：Germination Strain fungus block is put into breeding bag in step 7, and every bag point puts weight≤2Kg.
The fineness of fine sand is≤20 mesh in step 8.
The present invention has following advantageous effect：Because this method is to being mixed with the Germination Strain fritter of seeds of Gastrodia elata, by educating
Cultivated twice in kind of bag, can form the mycoderm of brown on Germination Strain fritter surface, this brown mycoderm to seeds of Gastrodia elata and can be sprouted
Bacterium germination forms protective layer, thus can reach drought-enduring and moisture-proof effect, solves the problems, such as that conventional breeding processes germination rate is low, has
There is drought-enduring, moisture-proof, improve the remarkable result of seed germination rate.
Embodiment
Embodiment 1
Step 1, in upper in July, 1 traditionally cultivated close ring bacterium bacterium material；
Step 2, traditionally to cultivate in upper in November, 1 wood sawdust culture medium Germination Strain polybag strain stand-by；
Step 3, upper in November, 1 select 200~250g/ weight no disease and pests harm rhizoma Gastrodiae protected under the conditions of natural temperature
Hide；
Step 4, in the first tenday period of a month January next year, by traditionally vernalization and the artificial pollination of the rhizoma Gastrodiae of institute's preservation, harvesting late March
Rhizoma Gastrodiae modeling fruit will be split；
Step 5, step 2 stand-by polybag strain point broken it is stand-by for the Germination Strain fritter of 2cm sizes；
Step 6, step 4 will be split into rhizoma Gastrodiae capsule break into two with one's hands and take seeds of Gastrodia elata to admix in the stand-by Germination Strain fritter of step 5, admix
When, the seeds of Gastrodia elata of 15 rhizoma Gastrodiae capsules is admixed by every Kg modeling bacterium germination fritters, and allow seeds of Gastrodia elata to be uniformly distributed to be adhered to and sprout
Bacterium germination fritter surrounding；
Step 7, the above-mentioned Germination Strain fungus block mixed is put into close in breeding bag and stays airing hole, by upper and lower one group of counterpart two bag
It is placed in breeding chamber, is cultivated 4 days under 20 DEG C of dry environments；
Step 8, above-mentioned cultivate after surface forms the Germination Strain fungus block unpacking of dense white hypha admixed into fine sand, make strain block
Surface sticks the grains of sand；
Step 9, the Germination Strain fritter that will be stained with the grains of sand load breeding bag, and opening is put into breeding chamber and is put and amassed wealth by heavy taxation by every bag of interval 2cm
Educate 20 days, cover 2cm fine sand after putting on breeding bag, fine sand humidity is maintained at 40%, when humidity is too low during cultivation, spray
Water moisturizing, form auburn mycoderm on Germination Strain fritter surface after 20 days and can be used to field planting.
Germination Strain fungus block is put into breeding bag in step 7, and every bag point puts weight≤2Kg.
The fineness of fine sand is≤20 mesh in step 8.
Embodiment 2
Step 1, in upper in July, 1 traditionally cultivated close ring bacterium bacterium material；
Step 2, traditionally to cultivate in upper in November, 1 wood sawdust culture medium Germination Strain polybag strain stand-by；
Step 3, no disease and pests harm rhizoma Gastrodiae preservation under the conditions of natural temperature in 220g/ weight of selection upper in November, 1；
Step 4, in the first tenday period of a month January next year, by traditionally vernalization and the artificial pollination of the rhizoma Gastrodiae of institute's preservation, harvesting late March
Rhizoma Gastrodiae modeling fruit will be split；
Step 5, step 2 stand-by polybag strain point broken it is stand-by for the Germination Strain fritter of 2cm sizes；
Step 6, step 4 will be split into rhizoma Gastrodiae capsule break into two with one's hands and take seeds of Gastrodia elata to admix in the stand-by Germination Strain fritter of step 5, admix
When, the seeds of Gastrodia elata of 18 rhizoma Gastrodiae capsules is admixed by every Kg modeling bacterium germination fritters, and allow seeds of Gastrodia elata to be uniformly distributed to be adhered to and sprout
Bacterium germination fritter surrounding；
Step 7, the above-mentioned Germination Strain fungus block mixed is put into close in breeding bag and stays airing hole, by upper and lower one group of counterpart two bag
It is placed in breeding chamber, is cultivated 5 days under 22 DEG C of dry environments；
Step 8, above-mentioned cultivate after surface forms the Germination Strain fungus block unpacking of dense white hypha admixed into fine sand, make strain block
Surface sticks the grains of sand；
Step 9, the Germination Strain fritter that will be stained with the grains of sand load breeding bag, and opening is put into breeding chamber and is put and amassed wealth by heavy taxation by every bag of interval 2cm
Educate 20 days, cover 2cm fine sand after putting on breeding bag, fine sand humidity is maintained at 45%, when humidity is too low during cultivation, spray
Water moisturizing, form auburn mycoderm on Germination Strain fritter surface after 20 days and can be used to field planting.
Germination Strain fungus block is put into breeding bag in step 7, and every bag point puts weight≤2Kg.
The fineness of fine sand is≤20 mesh in step 8.
Embodiment 3
Step 1, in upper in July, 1 traditionally cultivated close ring bacterium bacterium material；
Step 2, traditionally to cultivate in upper in November, 1 wood sawdust culture medium Germination Strain polybag strain stand-by；
Step 3, no disease and pests harm rhizoma Gastrodiae preservation under the conditions of natural temperature in 250g/ weight of selection upper in November, 1；
Step 4, in the first tenday period of a month January next year, by traditionally vernalization and the artificial pollination of the rhizoma Gastrodiae of institute's preservation, harvesting late March
Rhizoma Gastrodiae modeling fruit will be split；
Step 5, step 2 stand-by polybag strain point broken it is stand-by for the Germination Strain fritter of 2cm sizes；
Step 6, step 4 will be split into rhizoma Gastrodiae capsule break into two with one's hands and take seeds of Gastrodia elata to admix in the stand-by Germination Strain fritter of step 5, admix
When, the seeds of Gastrodia elata of 20 rhizoma Gastrodiae capsules is admixed by every Kg modeling bacterium germination fritters, and allow seeds of Gastrodia elata to be uniformly distributed to be adhered to and sprout
Bacterium germination fritter surrounding；
Step 7, the above-mentioned Germination Strain fungus block mixed is put into close in breeding bag and stays airing hole, by upper and lower one group of counterpart two bag
It is placed in breeding chamber, is cultivated 7 days under 25 DEG C of dry environments；
Step 8, above-mentioned cultivate after surface forms the Germination Strain fungus block unpacking of dense white hypha admixed into fine sand, make strain block
Surface sticks the grains of sand；
Step 9, the Germination Strain fritter that will be stained with the grains of sand load breeding bag, and opening is put into breeding chamber and is put and amassed wealth by heavy taxation by every bag of interval 2cm
Educate 20 days, cover 2cm fine sand after putting on breeding bag, fine sand humidity is maintained at 50%, when humidity is too low during cultivation, spray
Water moisturizing, form auburn mycoderm on Germination Strain fritter surface after 20 days and can be used to field planting.
Germination Strain fungus block is put into breeding bag in step 7, and every bag point puts weight≤2Kg.
The fineness of fine sand is≤20 mesh in step 8.
Claims (3)
1. improve the method for seed germination rate in a kind of gastrodia elata sexual propagation breeding process, including traditionally vernalization and artificial
Pollination, it is characterised in that：
Step 1, in upper in July, 1 traditionally cultivated close ring bacterium bacterium material；
Step 2, traditionally to cultivate in upper in November, 1 wood sawdust culture medium Germination Strain polybag strain stand-by；
Step 3, upper in November, 1 select 200~250g/ weight no disease and pests harm rhizoma Gastrodiae protected under the conditions of natural temperature
Hide；
Step 4, in the first tenday period of a month January next year, by traditionally vernalization and the artificial pollination of the rhizoma Gastrodiae of institute's preservation, harvesting late March
Rhizoma Gastrodiae modeling fruit will be split；
Step 5, step 2 stand-by polybag strain point broken it is stand-by for the Germination Strain fritter of 2cm sizes；
Step 6, step 4 will be split into rhizoma Gastrodiae capsule break into two with one's hands and take seeds of Gastrodia elata to admix in the stand-by Germination Strain fritter of step 5, admix
When, the seeds of Gastrodia elata of 15~20 rhizoma Gastrodiae capsules is admixed by every Kg modeling bacterium germination fritters, and allow seeds of Gastrodia elata to be uniformly distributed adhesion
In Germination Strain fritter surrounding；
Step 7, the above-mentioned Germination Strain fungus block mixed is put into close in breeding bag and stays airing hole, by upper and lower one group of counterpart two bag
It is placed in breeding chamber, is cultivated 4~7 days under 20~25 DEG C of dry environments；
Step 8, above-mentioned cultivate after surface forms the Germination Strain fungus block unpacking of dense white hypha admixed into fine sand, make strain block
Surface sticks the grains of sand；
Step 9, the Germination Strain fritter that will be stained with the grains of sand load breeding bag, and opening is put into breeding chamber and is put and amassed wealth by heavy taxation by every bag of interval 2cm
Educate 20 days, cover 2cm fine sand after putting on breeding bag, fine sand humidity is maintained at 40%~50% during cultivation, and humidity is too low
When, moisturizing of spraying water, form auburn mycoderm on Germination Strain fritter surface after 20 days and can be used to field planting.
2. improving the method for seed germination rate in a kind of gastrodia elata sexual propagation breeding process according to claim 1, it is special
Sign is：Germination Strain fungus block is put into breeding bag in step 7, and every bag point puts weight≤2Kg.
3. improving the method for seed germination rate in a kind of gastrodia elata sexual propagation breeding process according to claim 1, it is special
Sign is：The fineness of fine sand is≤20 mesh in step 8.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201610772675.7A CN107771667B (en) | 2016-08-31 | 2016-08-31 | Method for improving seed germination rate in gastrodia elata sexual propagation breeding process |
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| CN201610772675.7A CN107771667B (en) | 2016-08-31 | 2016-08-31 | Method for improving seed germination rate in gastrodia elata sexual propagation breeding process |
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| CN107771667B CN107771667B (en) | 2021-06-08 |
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Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1262864A (en) * | 1999-11-27 | 2000-08-16 | 王学宪 | Sexual reproductive breeding method of gastrodia elata |
| CN1473459A (en) * | 2003-08-07 | 2004-02-11 | 广水市东晨菌业科技有限公司 | Synchronously cultivating method for rhizoma gastrodiac sexual propagation |
| CN103548562A (en) * | 2013-10-23 | 2014-02-05 | 关岭百龙汇生物科技有限公司 | Gastrodia elata sexual propagation high-yield cultivation method |
| CN104663404A (en) * | 2015-03-09 | 2015-06-03 | 昭通市林业科学研究所 | Breeding method for gastrodia elata |
| CN105794495A (en) * | 2016-03-18 | 2016-07-27 | 中国科学院昆明植物研究所 | Armillaria mellea strain symbiotic with rhizoma gastrodiae and application of stain |
-
2016
- 2016-08-31 CN CN201610772675.7A patent/CN107771667B/en active Active
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1262864A (en) * | 1999-11-27 | 2000-08-16 | 王学宪 | Sexual reproductive breeding method of gastrodia elata |
| CN1473459A (en) * | 2003-08-07 | 2004-02-11 | 广水市东晨菌业科技有限公司 | Synchronously cultivating method for rhizoma gastrodiac sexual propagation |
| CN103548562A (en) * | 2013-10-23 | 2014-02-05 | 关岭百龙汇生物科技有限公司 | Gastrodia elata sexual propagation high-yield cultivation method |
| CN104663404A (en) * | 2015-03-09 | 2015-06-03 | 昭通市林业科学研究所 | Breeding method for gastrodia elata |
| CN105794495A (en) * | 2016-03-18 | 2016-07-27 | 中国科学院昆明植物研究所 | Armillaria mellea strain symbiotic with rhizoma gastrodiae and application of stain |
Non-Patent Citations (1)
| Title |
|---|
| 李世全等: "天麻优质高产栽培技术之(三)――天麻的有性繁殖 ", 《陕西林业》 * |
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