CN107746819A - A kind of method of efficiently pilot scale culture algae - Google Patents

A kind of method of efficiently pilot scale culture algae Download PDF

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Publication number
CN107746819A
CN107746819A CN201711106887.2A CN201711106887A CN107746819A CN 107746819 A CN107746819 A CN 107746819A CN 201711106887 A CN201711106887 A CN 201711106887A CN 107746819 A CN107746819 A CN 107746819A
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algae
culture
culture pond
efficiently
pilot scale
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李运广
罗玮
金连胜
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Wuhan Algae Biotechnology Co Ltd
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Wuhan Algae Biotechnology Co Ltd
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Priority to CN201711106887.2A priority Critical patent/CN107746819A/en
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/12—Unicellular algae; Culture media therefor

Abstract

The invention provides a kind of method of efficiently pilot scale culture algae, including：To culture pond pretreatment and culture water pretreatment；Culture medium is added into culture pond, and algae kind is seeded in culture pond by inoculum density；Monitoring algae group size and luminous intensity in culture pond, temperature, pH value and frustule PS II photochemical activity index in real time；Protection regulation measure is carried out according to Monitoring Data.The present invention make algae growth each period can Effective Suspension, efficiently utilize light, nutrition and CO2, so as to realize fast-growth.The algae bio amount obtained can produce functional food, raw-food material, bait, high added value secondary metabolite and biodiesel etc..

Description

A kind of method of efficiently pilot scale culture algae
Technical field
The present invention relates to algae production technical field, more particularly to a kind of method of efficiently pilot scale culture algae.
Background technology
Algae is the low photoautotroph of no root, stem and leaf differentiation, and it has, and individual is small, photosynthetic efficiency is high, adapts to environment The characteristics of scope is extensively and yield per unit area is high.Algae scale evaluation and comprehensive development and utilization be solve facing mankind grain, One of effective measures of problems such as the energy, environment and health.As commercially produced chlorella, spirulina, Haematococcus pluvialis and Dunaliella salina etc., it is possible to provide the material such as high-quality protein, lutein, astaxanthin, EPA and DHA, be high-quality work( Can property food and bait.
Runway pool culture pond is current most economical and effective outdoor algae pilot scale culture facility, has operating cost It is low, be easy to manage and the features such as easily amplify, be most widely used in current algae pilot scale culture.Spiral is commonly used in raceway pond Paddle wheel blade stirs water body, and to drive algae to be suspended in water body, this mode is highly effective to most of unicellular microalgaes.But Be to it is some form the algae of large population for, due to individual it is larger, sedimentation it is very fast, it is especially larger in area In raceway pond, it is very universal that algae in local waters sinks to the bottom phenomenon.Also, with incrementally increasing for algae colony, sink to the bottom phenomenon More serious, these sink to the bottom algae, gradually dead because not receiving sunlight for a long time, so as to breed bacteria and water body is degenerated, give Cultivation brings heavy losses.
On the other hand, CO2Play an important roll in algae culture, it is not only algae and carries out photosynthetic important bottom Thing still adjusts culture water body pH optimal selection.In incubation, as algae density increases, water body pH can gradually rise It is high.Such as when outdoor is using sunshine production, when fine, in the period at daily high noon, the pH value of culture pond is reachable More than 11.Injury can be produced to algae by crossing high ph-values, or even kill cell.Generally, CO can be supplied in pond2, still In order to which algae can obtain enough light, the water depth in racetrack culture pond is generally kept in below 30cm.So shallow water, So that CO2Residence time in water is too short, it is impossible to is effectively dissolved in water body, causes that pH can not be reduced in time, and produce very It is big to waste.
In addition, the just important factor of algal grown.Luminous energy is insufficient, can reduce algal grown speed, and light intensity mistake Qiang Zehui generation photoinhibitions, harm is produced to algae, and serious also results in alga cells albefaction and death.Therefore, exist During cultivating algae using natural light, in good time shading is particularly important.
The content of the invention
For the drawbacks described above and problem of prior art, the purpose of the embodiment of the present invention is to provide a kind of efficiently scale training The method for supporting algae.The method of the present invention efficiently solves the CO in jumpbogroup body algae precipitation, culture pond2The residence time in water Short, dissolution rate is low, it is impossible to provides enough carbon sources for algae photosynthesis, and can not effectively maintain appropriate intensity of illumination, shadow Ring the technical problem of algal grown speed.
In order to achieve the above object, the present invention provides following technical scheme：
A kind of method of efficiently pilot scale culture algae of the present invention, comprises the following steps：
Step 1: using runway pool culture pond, to culture pond pretreatment and culture water pretreatment；
Step 2: adding culture medium into culture pond, and algae kind is seeded in culture pond by inoculum density；
Step 3: monitoring algae group size and luminous intensity in culture pond, temperature, pH value and frustule PS II in real time Photochemical activity index；
Step 4: the Monitoring Data in step 3 carries out protection regulation measure.
Further, the protection regulation measure includes：When the algae colony diameter that monitoring obtains is more than diameter criteria value, Algae colony is stirred and ventilation treatment；When the frustule PS II monitored photochemical activity is less than photochemical activity During standard value, shading treatment is carried out；When the pH value monitored is higher than pH critical fields, CO is passed through to culture pond bottom2Gas.
Stir process and ventilation treatment are combined by the present invention, can effectively avoid the deposited phenomenon of jumpbogroup body algae.
Further, in step 1, culture pond pretreatment includes cleaning the surrounding enviroment of culture pond and culture pond, Sterilize and be exposed to the sun；
Culture pond is pre-processed, culture pond is had the interior environment of a relative closure, similar construction greenhouse can be used Mode is isolated；Surrounding enviroment want periodic cleaning and sterilization, keep drying and clean, not the space to other miscellaneous algae propagations. 1-3% bleaching powder disinfectant can be sprayed in culture pond, is then exposed to the sun under fair weather 1-3 days.
Culture includes filtering culture with water with water pretreatment, during filtering filter membrane of the usage aperture less than 3um or Using fine sand, charcoal as inserts filtration facility；
Culture is preferably selected more than underground 200m deep water, running water with water, and this water can be used directly；In this way other The natural water in source then needs to filter, to remove other planktonic organisms.
Wherein, the culture water plus water depth in 10-50cm；Culture water is needed by stirring by least 12h Dynamic or Air Exposure.
Further, in step 2, the culture medium is included by mass fraction：NaNO3100-2000 parts, Ca (H2PO4)2 40-500 parts, K2Mg(SO4)260-300 parts, Na2SiO310-1000 parts, Na2CO320-300 parts, FeCl23-20 parts, H3BO31-10 parts, MnCl2·4H2O 0.5-5 parts, ZnSO4·7H2O 0.1-0.6 parts, CuSO4·5H2O 0.02-0.2 parts, Na2MoO4·2H2O 0.02-1.0 parts, CoCl2·6H2O 0.02-4 parts.
Further, in step 2, algae kind need to combine culture light intensity and temperature conditionss carry out domestication 7-8 days before inoculation； The frond diameter of inoculation is more than 400um, and the fresh weight of density is not less than 0.5kgm-3。
Algae kind is tamed, to adapt to condition of culture, avoids the stress reaction caused by environment changes from occurring；Domestication When should follow gradual change principle, allow algae progressively to adapt to environment.
Further, propeller type wheel blade agitating device and bubble type air-breather are also included in the culture pond, to algae Colony is stirred and ventilation treatment；In step 4, the diameter criteria value is 0.5mm, and when algae, colony is a diameter of When between 0.5mm-1mm, propeller type wheel blade agitating device is only opened, and takes turns slurry rotating speed and is not less than 12rpm；When algae colony diameter When between 1mm-3mm, wheel slurry rotating speed is not less than 10rpm；When algae colony diameter is more than 3mm, wheel slurry rotating speed be adjusted to 6rpm with Under, and bubble type air-breather is opened, ventilate and heave bottom frond.
It can so ensure that cultivating algae does not produce precipitation, avoids because sinking to the bottom for a long time, frond caused by illumination deficiency point Solve, be dead and putrid and deteriorated；And the algae of large population not destroy caused by too strong mechanical agitation by shearing force etc..
Further, in step 4, the photochemical activity standard value is 0.2；When frustule PS II photochemical activity During less than 0.2, using shade net shading, frustule PS II photochemical activity is set to return to more than 0.4 in 1h.
A kind of lower-cost Photochemical Efficiency analyzer is utilized in the present invention, completes what light was endangered algae in 30s Detection, according to detection parameters, determine whether to take shading measure, so as to avoid strong light from producing harm to the algae of culture, while really It is fixed when to remove sunshade net, make algae that there is the time of longer suitable growth, to obtain maximum algae bio amount.
Further, in step 4, the pH critical fields are 6.5-9.5；When the pH value monitored is higher than 9.5, beat The bubble type air-breather is driven, CO is passed through to culture pond bottom using nanometer oxygen increasing tube2Gas, and the CO being passed through2Gas bubbles Size is between 10-50 μm；When pH value is less than 6.5, stop CO2Gas is passed through.
Increase CO2Supply, there is provided the inorganic carbon source needed for algae photosynthesis, accelerate photosynthetic carbon fixation speed, given birth to for algae It is long that sufficient carbon skeleton is provided.
Further, in step 4, in addition to algae is harvested or transfer processing；When the density of algae is more than fresh weight 10kg/m-3Afterwards, satisfactory algae is harvested using mesh screen, reduces algae density.
When algae density is more than fresh weight 10kg/m-3Afterwards, serious from shading between frond, culture algae can give birth to because cannot get substantial light Length is slack-off；Now need by way of harvesting or shifting, reduce the density of algae, Chi Zhongzao density is maintained at appropriate water It is flat, avoid causing frond too low from shading or density and yield declines caused by producing Xanthophyll cycle etc. because density is too high, so as to Keep higher production efficiency.
Further, the bubble type air-breather includes anti-precipitation breather pipe and CO2Gas ventilation pipe；The anti-precipitation Breather pipe is passed through air or nitrogen, and it is 0.2m-1mm that it, which rouses Air Bubble Size, to stir algae in V-groove, avoids precipitation from producing；Institute State CO2Gas ventilation pipe is nanometer oxygen increasing tube, is passed through CO2Gas, and the CO being passed through2Gas bubbles size 10-50 μm it Between, to increase CO2Dissolved efficiency；
Culture pond has two sets of independent stirring systems of propeller type wheel blade agitating device and bubble type air-breather, and the two is mutual For supplement；Starch the throughput of rotating speed and air-blowing device by left regulation wheel respectively in culture algae different size period so that culture Algae does not produce precipitation, and shearing force etc. is not destroyed caused by too strong mechanical agitation for algae colony.Above-mentioned anti-precipitation ventilation Pipe can be passed through other inert gases such as air or nitrogen.
The culture pond bottom sets multiple descending V-type bendways, and per 300-500m in culture pond2One is set on area The individual descending V-type bendway；Water slot opening 0.1-0.5m, deep 0.1-2m, the long 2-4m of the descending V-type bendway.
The present invention sets up descending V-type bendway at culture pond bottom, can be effectively increased CO2The residence time in water, increase CO2Dissolving, supply efficiency is improved, sufficient carbon source is provided for algae photosynthesis.
Also distribution sets Qi Shi areas in the culture pond of the present invention, for carrying out Air Exposure to culture water and preventing algae from sinking Form sediment.The 0.5-1m in culture pond2In the range of a gas stone is set, caused Air Bubble Size is advisable in the range of 0.8-1.5mm, and And the distribution of gas stone should be arranged and is staggered, frond, successively by the gas stone being interspersed, ensures in the case where wheel slurry agitation force promotes All algaes can suspend.
A kind of method of efficiently pilot scale culture algae provided by the invention, its advantage are：
1st, the energy Effective Suspension of each period of the invention for making algae in growth, efficiently utilizes light, nutrition and CO2, so as to Realize fast-growth.It is secondary that the algae bio amount obtained can produce functional food, raw-food material, bait, high added value Metabolite and biodiesel etc..
2nd, the present invention can algae density in Reasonable Regulation And Control culture pond, effectively avoid miscellaneous algae pollution, improve production efficiency；With Algae growth is cultivated, algae density is reduced by way of removing or harvesting, avoids frond because density is too high, mutual shading causes life Length is slack-off.
3rd, two kinds of agitation modes of bubble type air-breather and propeller type wheel blade agitating device are effectively tied in culture pond Altogether, avoid in culture pond because of simple ventilation or simple wheel slurry stirring and caused by a large amount of deposited phenomenons of frond, make algae Energy Effective Suspension, efficient light, fast-growth.
4th, as frond is progressively grown up, the wheel slurry rotating speed of propeller type wheel blade agitating device is gradually reduced, while is equipped with drum The air agitation of bubble air-breather, it can effectively avoid the mechanical damage of big colony.
Brief description of the drawings
In order to illustrate more clearly about the embodiment of the present invention or technical scheme of the prior art, below will be to embodiment or existing There is the required accompanying drawing used in technology description to be briefly described, it should be apparent that, drawings in the following description are only this Some embodiments of invention, for those of ordinary skill in the art, without having to pay creative labor, may be used also To obtain other accompanying drawings according to these accompanying drawings.
Fig. 1 is the overlooking the structure diagram of culture pond in a kind of efficiently method of pilot scale culture algae of the invention；
Fig. 2 is gas stone arrangement schematic diagram in a kind of efficiently culture pond of the method for pilot scale culture algae of the present invention；
Fig. 3 is the descending V-type bendway knot in a kind of efficiently culture pond of the method for pilot scale culture algae of the present invention Structure schematic diagram.
Embodiment
Below in conjunction with embodiments of the invention, technical scheme is clearly and completely described, it is clear that Described embodiment is only part of the embodiment of the present invention, rather than whole embodiments.Based on the implementation in the present invention Example, the every other embodiment that those of ordinary skill in the art are obtained under the premise of creative work is not made, is belonged to The scope of protection of the invention.
As Figure 1-3, the method for a kind of efficiently pilot scale culture algae of the invention, comprises the following steps：
Step 1: using runway pool culture pond 1, culture pond 1 is pre-processed and culture water pretreatment；
The pretreatment of culture pond 1 includes the surrounding enviroment of culture pond 1 and culture pond 1 are cleaned, sterilized and are exposed to the sun；
Culture includes filtering culture with water with water pretreatment, during filtering filter membrane of the usage aperture less than 3um or Using fine sand, charcoal as inserts filtration facility；
Above-mentioned culture water adds water depth in 10-50cm；Culture water need by by least 12h agitation or Air Exposure.
Step 2: adding culture medium into culture pond 1, and algae kind is seeded in culture pond 1 by inoculum density；
Above-mentioned culture medium is included by mass fraction：NaNO3100-2000 parts, Ca (H2PO4)240-500 parts, K2Mg (SO4)260-300 parts, Na2SiO310-1000 parts, Na2CO320-300 parts, FeCl23-20 parts, H3BO31-10 parts, MnCl2·4H2O 0.5-5 parts, ZnSO4·7H2O 0.1-0.6 parts, CuSO4·5H2O 0.02-0.2 parts, Na2MoO4·2H2O 0.02-1.0 parts, CoCl2·6H2O 0.02-4 parts.
Algae kind need to combine culture light intensity and temperature conditionss carry out domestication 7-8 days before inoculation；The frond diameter of inoculation is more than 400um, the fresh weight of density are not less than 0.5kgm-3。
Step 3: algae group size and luminous intensity, temperature, pH value and frustule PS II in culture pond 1 are monitored in real time Photochemical activity index；
Step 4: the Monitoring Data in step 3 carries out protection regulation measure；
Above-mentioned protection regulation measure includes：When the algae colony diameter that monitoring obtains is more than diameter criteria value, to algae colony It is stirred and ventilation treatment；When the frustule PS II monitored photochemical activity is less than photochemical activity standard value When, carry out shading treatment；When the pH value monitored is higher than pH critical fields, CO is passed through to culture pond bottom2Gas.
Also include propeller type wheel blade agitating device and bubble type air-breather in above-mentioned culture pond 1, algae colony is carried out Stir process and ventilation treatment；Above-mentioned diameter criteria value is 0.5mm, when between a diameter of 0.5mm-1mm of algae colony, is only opened Propeller type wheel blade agitating device, and its wheel 5 rotating speed of slurry is not less than 12rpm；When between a diameter of 1mm-3mm of algae colony, wheel Starch 5 rotating speeds and be not less than 10rpm；When algae colony diameter is more than 3mm, wheel 5 rotating speeds of slurry are adjusted to below 6rpm, and open bubble type and lead to Device of air, ventilate and heave bottom frond.
Above-mentioned photochemical activity standard value is 0.2；When frustule PS II photochemical activity is less than 0.2, using sheltering from heat or light Net shading, frustule PS II photochemical activity is set to return to more than 0.4 in 1h.
Above-mentioned pH critical fields are 6.5-9.5；When the pH value monitored is higher than 9.5, above-mentioned bubble type ventilation dress is opened Put, CO is passed through to culture pond bottom using nanometer oxygen increasing tube2Gas, and the CO being passed through2Gas bubbles size is between 10-50 μm； When pH value is less than 6.5, stop CO2Gas is passed through.
Also include harvesting algae or transfer processing；When the density of algae is more than fresh weight 10kg/m-3Afterwards, using mesh screen pair Satisfactory algae is harvested, and reduces algae density.
As Figure 1-3, the culture pond 1 in cultural method of the invention, culture pond 1 are in runway shape；Set in culture pond 1 Propeller type wheel blade agitating device and bubble type air-breather；Above-mentioned bubble type air-breather includes anti-precipitation breather pipe and CO2 Gas ventilation pipe；Above-mentioned anti-precipitation breather pipe is passed through air or nitrogen, and it is 0.2m-1mm that it, which rouses Air Bubble Size, to stir V-groove Middle algae, precipitation is avoided to produce；Above-mentioned CO2Gas ventilation pipe is nanometer oxygen increasing tube, is passed through CO2Gas, and the CO being passed through2Gas Body Air Bubble Size is between 10-50 μm, to increase CO2Dissolved efficiency.
The above-mentioned bottom of culture pond 1 sets multiple descending V-type bendways 3, and per 300-500m in culture pond 12Set on area Put a descending V-type bendway 3；Water slot opening 0.1-0.5m, deep 0.1-2m, the long 2- of above-mentioned descending V-type bendway 3 4m, as shown in Figure 3.
Also distribution sets Qi Shi areas 2 in the culture pond 1 of the present embodiment, for carrying out Air Exposure to culture water and preventing algae Class precipitates.The 0.5-1m in culture pond 12In the range of set a gas stone 201, caused Air Bubble Size is advisable in 1mm, and gas The distribution of stone 201 should be arranged and is staggered, and frond, successively by the Qi Shi areas 2 being interspersed, is protected in the case where wheel 5 agitation forces of slurry promote Demonstrate,proving all algaes can suspend, and the setting that is interspersed of gas stone 201 is as shown in Figure 2.
Embodiment：
By taking a kind of production for the nostoc (Nostoc sp.) that can form big colony as an example, algae kind picks up from Guilin volt ripple Mountain, purifying algae kind is obtained after this laboratory isolates and purifies.
Used medium is preferred culture medium：NaNO3 0.1g/L-1, Ca (H2PO4)2 0.1g/L-1, K2Mg(SO4)20.08g/L-1, Na2SiO3 0.05g/L-1,Na2CO3 0.15g/L-1,FeCl2 4mg/L-1, H3BO3 8mg/L-1, MnCl2· 4H2O 5mg/L-11,ZnSO4·7H2O 0.3mg/L-1,CuSO4·5H2O 0.1mg/L-1,Na2MoO4·2H2O 0.04mg/L-1, CoCl2·6H2O 0.02mg/L-1。
Racetrack culture pond 1 used is long 70m × wide 12m × deep 0.5m, the reverse V-shaped steel structure skeleton of overhead, and the mesh of overlying 60 is prevented Worm net, 1m places add open and flat formula sunshade net above fly net highest point, and sunshade net is motorized adjustment, under fair weather, daily 11: 30-13:30 carry out 80% sunshade, remove thereafter.
The wheel slurry 5 of unilateral placement propeller type wheel blade agitating device in pond, except 10m is Air-free stone area 4 before and after wheel slurry 5, its Leeway side's arrangement gas stone 201, with, at intervals of 0.5m, per line spacing 1m, in the ranks, gas stone 201 interlocks difference between promoting the circulation of qi stone 201 Distribution.Two long 4m × wide 0.3m × deep 1m descending V-type bendway 3, groove top portion are laid in two diagonal angles of culture pond 1 Place laying 24 eye mesh screens concordant with bottom of pond, it can play a part of preventing algae from sinking to tank, bottom land places bubble type ventilation One root long 3m of device CO2The gas stone 201 of gas-nano oxygenation breather pipe and 11 a diameter of 3cm, each leads into 99%CO2 And air.Wherein CO2Duration of ventilation and flow must close, when pH is more than 9.5 trends of the times according to depending on pH when pH is less than 6.5 It must open, CO2Gas ventilation pipe duration of ventilation is consistent with wheel 5 available machine times of slurry, and daily 10-12h, night stops supplying.
The river finally through 3 μm of membrane filtrations, depth of water 40cm, per 3-5d moisturizings once, to keep are put into culture pond 1 The depth of water is stable.Each nutritional components are added by above-mentioned preferred culture medium formula, the plan for accessing a diameter of φ 0.8mm through domestication is spherical Nostoc 130kg, density are about 0.5kg/m-3；Wheel 5 rotating speeds of slurry are set to 14rpm, and start shooting 10-12h daily, wherein being shut down every 2h 15min, to increase motor service life；After 15d, diameter reaches 1.1mm, now adjustment of rotational speed to 11rpm；After 35 days, diameter Reach 2.4mm, density is about 12kg/m-3, now separate that 1000kg algaes are pool cultivated to other, and density is adjusted to 7-8kg/m-3； After 50d, diameter increases to 3mm, separates that 1500kg algaes are pool cultivated to other, and density is adjusted to about 8kg/m-3, this hour wheel slurry 5 rotating speeds tune It is whole to 5rpm, bubble type air-breather is opened；After 70d, diameter increases to 4.0-4.5cm, harvests algae about 5000kg fresh weights.
The foregoing is only a specific embodiment of the invention, but protection scope of the present invention is not limited thereto, any Those familiar with the art the invention discloses technical scope in, change or replacement can be readily occurred in, should all be contained Cover within protection scope of the present invention.Therefore, protection scope of the present invention described should be defined by scope of the claims.

Claims (10)

  1. A kind of 1. method of efficiently pilot scale culture algae, it is characterised in that：Comprise the following steps：
    Step 1: using runway pool culture pond, to culture pond pretreatment and culture water pretreatment；
    Step 2: adding culture medium into culture pond, and algae kind is seeded in culture pond by inoculum density；
    Step 3: monitoring algae group size and luminous intensity in culture pond, temperature, pH value and frustule PS's II in real time is photochemical Learn activity index；
    Step 4: the Monitoring Data in step 3 carries out protection regulation measure.
  2. A kind of 2. method of efficiently pilot scale culture algae according to claim 1, it is characterised in that：The protection regulation Measure includes：When the obtained algae colony diameter of monitoring is more than diameter criteria value, algae colony is stirred and ventilation at Reason；When the frustule PS II monitored photochemical activity is less than photochemical activity standard value, shading treatment is carried out；Work as monitoring When the pH value arrived is higher than pH critical fields, CO is passed through to culture pond bottom2Gas.
  3. A kind of 3. method of efficiently pilot scale culture algae according to claim 1, it is characterised in that：In step 1, training Supporting pond pretreatment includes the surrounding enviroment of culture pond and culture pond are cleaned, sterilized and are exposed to the sun；
    Culture includes filtering culture with water with water pretreatment, filter membrane of the usage aperture less than 3um or with thin during filtering Husky, charcoal is the filtration facility of inserts；
    Wherein, the culture water plus water depth in 10-50cm；Culture water need by by least 12h agitation or Person's Air Exposure.
  4. A kind of 4. method of efficiently pilot scale culture algae according to claim 1, it is characterised in that：In step 2, institute State culture medium is included by mass fraction：NaNO3100-2000 parts, Ca (H2PO4)240-500 parts, K2Mg(SO4)2 60-300 Part, Na2SiO310-1000 parts, Na2CO320-300 parts, FeCl23-20 parts, H3BO31-10 parts, MnCl2·4H2O 0.5-5 Part, ZnSO4·7H2O 0.1-0.6 parts, CuSO4·5H2O 0.02-0.2 parts, Na2MoO4·2H2O 0.02-1.0 parts, CoCl2· 6H2O 0.02-4 parts.
  5. A kind of 5. method of efficiently pilot scale culture algae according to claim 1, it is characterised in that：In step 2, algae Kind need to combine culture light intensity and temperature conditionss carry out domestication 7-8 days before inoculation；The frond diameter of inoculation is more than 400um, density Fresh weight be not less than 0.5kgm-3。
  6. A kind of 6. method of efficiently pilot scale culture algae according to claim 1, it is characterised in that：In the culture pond Also include propeller type wheel blade agitating device and bubble type air-breather, algae colony is stirred and ventilation treatment；Step In rapid four, the diameter criteria value is 0.5mm, when between a diameter of 0.5mm-1mm of algae colony, only opens propeller type wheel blade Agitating device, and take turns slurry rotating speed and be not less than 12rpm；When between a diameter of 1mm-3mm of algae colony, wheel slurry rotating speed is not less than 10rpm；When algae colony diameter is more than 3mm, wheel slurry rotating speed is adjusted to below 6rpm, and opens bubble type air-breather, and ventilation will Bottom frond is heaved.
  7. A kind of 7. method of efficiently pilot scale culture algae according to claim 1, it is characterised in that：In step 4, institute Photochemical activity standard value is stated as 0.2；When frustule PS II photochemical activity is less than 0.2, using shade net shading, make algae Cell PS II photochemical activity returns to more than 0.4 in 1h.
  8. A kind of 8. method of efficiently pilot scale culture algae according to claim 6, it is characterised in that：In step 4, institute It is 6.5-9.5 to state pH critical fields；When the pH value monitored is higher than 9.5, the bubble type air-breather is opened, using nanometer Oxygen increasing tube is passed through CO to culture pond bottom2Gas, and the CO being passed through2Gas bubbles size is between 10-50 μm；When pH value is less than When 6.5, stop CO2Gas is passed through.
  9. A kind of 9. method of efficiently pilot scale culture algae according to claim 1, it is characterised in that：In step 4, also Including being harvested to algae or transfer processing；When the density of algae is more than fresh weight 10kg/m-3Afterwards, using mesh screen to satisfactory Algae is harvested, and reduces algae density.
  10. A kind of 10. method of efficiently pilot scale culture algae according to claim 6, it is characterised in that：The bubble type Air-breather includes anti-precipitation breather pipe and CO2Gas ventilation pipe；The anti-precipitation breather pipe is passed through air or nitrogen, its air-blowing Bubble size is 0.2m-1mm, to stir algae in V-groove, avoids precipitation from producing；The CO2Gas ventilation pipe is nano oxygen enriching Pipe, is passed through CO2Gas, and the CO being passed through2Gas bubbles size is between 10-50 μm, to increase CO2Dissolved efficiency；
    The culture pond bottom sets multiple descending V-type bendways, and per 300-500m in culture pond2One institute is set on area State descending V-type bendway；Water slot opening 0.1-0.5m, deep 0.1-2m, the long 2-4m of the descending V-type bendway.
CN201711106887.2A 2017-11-10 2017-11-10 A kind of method of efficiently pilot scale culture algae Pending CN107746819A (en)

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CN113526675A (en) * 2021-08-06 2021-10-22 苑春亭 Method for adjusting water quality of mariculture pond by using floating ecological enteromorpha

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Application publication date: 20180302