CN107746436A - A kind of method that enzyme liquid using sporotrichum thermophile extracts fragrant plant mentioned in ancient texts carragheen - Google Patents
A kind of method that enzyme liquid using sporotrichum thermophile extracts fragrant plant mentioned in ancient texts carragheen Download PDFInfo
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- CN107746436A CN107746436A CN201711177059.8A CN201711177059A CN107746436A CN 107746436 A CN107746436 A CN 107746436A CN 201711177059 A CN201711177059 A CN 201711177059A CN 107746436 A CN107746436 A CN 107746436A
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- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08B—POLYSACCHARIDES; DERIVATIVES THEREOF
- C08B37/00—Preparation of polysaccharides not provided for in groups C08B1/00 - C08B35/00; Derivatives thereof
- C08B37/0006—Homoglycans, i.e. polysaccharides having a main chain consisting of one single sugar, e.g. colominic acid
- C08B37/0036—Galactans; Derivatives thereof
- C08B37/0042—Carragenan or carragen, i.e. D-galactose and 3,6-anhydro-D-galactose, both partially sulfated, e.g. from red algae Chondrus crispus or Gigantia stellata; kappa-Carragenan; iota-Carragenan; lambda-Carragenan; Derivatives thereof
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- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08B—POLYSACCHARIDES; DERIVATIVES THEREOF
- C08B37/00—Preparation of polysaccharides not provided for in groups C08B1/00 - C08B35/00; Derivatives thereof
- C08B37/0003—General processes for their isolation or fractionation, e.g. purification or extraction from biomass
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Abstract
The invention discloses a kind of method that enzyme liquid using sporotrichum thermophile extracts fragrant plant mentioned in ancient texts carragheen, it is that upper strata enzyme clear liquid is collected by centrifugation after sporotrichum thermophile is cultivated in nutrient solution, then dry fragrant plant mentioned in ancient texts powder is mixed with gained enzyme clear liquid, it is filtered, wash, boil, freeze after, add hydrogen peroxide to boil, most afterwards through filtering to obtain carragheen.The inventive method is safe and environment-friendly, convenient, the environmental pollution that can effectively avoid traditional soda extraction carragheen from bringing, has very wide application prospect.
Description
Technical field
The invention belongs to food additives preparing technical field, and in particular to a kind of enzyme liquid using sporotrichum thermophile is extracted
The method of fragrant plant mentioned in ancient texts carragheen.
Background technology
Sporotrichum thermophile（Sporotrichum thermophile）It is a kind of thermophilic mould, the existence range in nature
It is wider.The mould growth is fast, the enzyme liquid of secretion is still active at high temperature, it is not easy to inactivates, thus has higher application prospect.
And also contain the endo cellulase of special high vigor in its enzyme liquid, more add commercial application potentiality.
With attention of the country to industrial discharge sewage, deposited using traditional soda processing fragrant plant mentioned in ancient texts to obtain the method for carragheen
Pollution is big, wastewater flow rate is more the shortcomings that, this just make exploitation it is new it is safe and environment-friendly, easily carragheen extractive technique becomes one
Kind there is an urgent need to.For this problem, the present invention develops a kind of enzyme liquid using sporotrichum thermophile and extracts fragrant plant mentioned in ancient texts carragheen
Method, it can effectively avoid the environmental pollution that alkali process is brought.
The content of the invention
It is an object of the invention to provide a kind of method that enzyme liquid using sporotrichum thermophile extracts fragrant plant mentioned in ancient texts carragheen, it can
The problem of environmental pollution for effectively avoiding traditional soda extraction carragheen from bringing, there is very wide application prospect.
To achieve the above object, the present invention adopts the following technical scheme that：
A kind of method that enzyme liquid using sporotrichum thermophile extracts fragrant plant mentioned in ancient texts carragheen, it comprises the following steps：
1）Sporotrichum thermophile is cultivated 10-12 days in nutrient solution, then centrifuges 5-10 minutes in refrigerated centrifuge, in collection
Layer enzyme clear liquid；
2）By dry fragrant plant mentioned in ancient texts grinds, 100-200 mesh sieves are crossed, add step 1）Gained enzyme clear liquid is mixed, and 2- is placed at 50-70 DEG C
5 days, then filter, the water with the weight such as dry fragrant plant mentioned in ancient texts added after residue washing 3-5 times, boil to sticky, then add the dilution of equivalent water,
The hydrogen peroxide for adding volumetric concentration 30% after chilled boils, and is kept for boil condition 3-10 minutes, filters, produces carragheen.
Step 1）Described in nutrient solution raw material composition be calculated as by weight：Sucrose 1-3 parts, (NH4)2SO4 0.1-0.3
Part, KH2PO40.1-0.3 parts, MgSO4•7H2O 0.01-0.05 parts, CaCl20.01-0.05 parts, peptone 0.1-0.5 parts, 1M
Urea 0.1-0.5 parts, Citric Acid Mono 0.1-0.5 parts, Na2HPO4•12H2O 0.5-1 parts, distilled water 80-100 parts.
Step 2）The dosage of each raw material includes by weight：Dry fragrant plant mentioned in ancient texts 10-30 parts, 100 parts of enzyme clear liquid, hydrogen peroxide 5-10
Part.
The remarkable advantage of the present invention is：
（1）The heat endurance of the more other enzymes of sporotrichum thermophile is strong, and catalytic activity at high temperature is high, has excellent characteristic.This
Invention utilizes a large amount of endo cellulases contained in sporotrichum thermophile enzyme liquid, thin further to destroy with reference to freezing heating repeatedly
Cell wall, so as to effectively extract the carragheen in fragrant plant mentioned in ancient texts cell, and do not have during it using the acid that may be polluted to environment
Or base reagent, it is a kind of environmentally friendly the problem of the environmental pollution that can effectively avoid traditional soda extraction carragheen from bringing
Carragheen extracting method.
（2）Boiling time is shorter in the present invention, can largely save the energy, and generation sewage is few, can greatly reduce enterprise
Cost of water treatment.
（3）Reaction condition of the present invention is gentle, without HTHP, it is not necessary to special reactor.
Embodiment
In order that content of the present invention easily facilitates understanding, with reference to embodiment to of the present invention
Technical scheme is described further, but the present invention is not limited only to this.
Embodiment 1
A kind of method that enzyme liquid using sporotrichum thermophile extracts fragrant plant mentioned in ancient texts carragheen, it comprises the following steps：
1）By 1 parts by weight of sucrose, 0.14 parts by weight (NH4)2SO4, 0.2 parts by weight KH2PO4, 0.03 parts by weight MgSO4•7H2O、
0.03 parts by weight CaCl2, 0.1 parts by weight peptone, 0.5 parts by weight 1M urea, 0.15 parts by weight Citric Acid Mono, 0.9 weight
Part Na2HPO4•12H2O, 100 parts by weight distilled water are made into nutrient solution；Sporotrichum thermophile is cultivated 10 days in nutrient solution, then
Centrifuged 10 minutes in refrigerated centrifuge, collect upper strata enzyme clear liquid；
2）By the dry fragrant plant mentioned in ancient texts grinds of 20 parts by weight, 100 mesh sieves are crossed, add 100 parts by weight steps 1）Gained enzyme clear liquid mixes, 50
Place 5 days at DEG C, then filter, the water of 20 parts by weight is added after residue washing 3 times, boil to sticky, then add 20 parts by weight
Water dilutes, and 5 parts by weight of chilled rear addition, the hydrogen peroxide of volumetric concentration 30% boil, and keep boil condition 5 minutes, filter,
Carragheen is produced, its recovery rate is 33%, compared with traditional soda（10%-20%）Significantly improve.
Embodiment 2
A kind of method that enzyme liquid using sporotrichum thermophile extracts fragrant plant mentioned in ancient texts carragheen, it comprises the following steps：
1）By 1 parts by weight of sucrose, 0.1 parts by weight (NH4)2SO4, 0.1 parts by weight KH2PO4, 0.01 parts by weight MgSO4•7H2O、
0.01 parts by weight CaCl2, 0.2 parts by weight peptone, 0.1 parts by weight 1M urea, 0.2 parts by weight Citric Acid Mono, 0.5 parts by weight
Na2HPO4•12H2O, 80 parts by weight distilled water are made into nutrient solution；Sporotrichum thermophile is cultivated 11 days in nutrient solution, then in cold
Freeze in centrifuge and centrifuge 5 minutes, collect upper strata enzyme clear liquid；
2）By the dry fragrant plant mentioned in ancient texts grinds of 10 parts by weight, 100 mesh sieves are crossed, add 100 parts by weight steps 1）Gained enzyme clear liquid mixes, 65
Place 3 days at DEG C, then filter, the water of 10 parts by weight is added after residue washing 3 times, boil to sticky, then add 10 parts by weight
Water dilutes, and 8 parts by weight of chilled rear addition, the hydrogen peroxide of volumetric concentration 30% boil, and keep boil condition 3 minutes, filter,
Carragheen is produced, its recovery rate is 37%, compared with traditional soda（10%-20%）Significantly improve.
Embodiment 3
A kind of method that enzyme liquid using sporotrichum thermophile extracts fragrant plant mentioned in ancient texts carragheen, it comprises the following steps：
1）By 3 parts by weight of sucrose, 0.3 parts by weight (NH4)2SO4, 0.3 parts by weight KH2PO4, 0.05 parts by weight MgSO4•7H2O、
0.05 parts by weight CaCl2, 0.5 parts by weight peptone, 0.3 parts by weight 1M urea, 0.5 parts by weight Citric Acid Mono, 1 parts by weight
Na2HPO4•12H2O, 90 parts by weight distilled water are made into nutrient solution；Sporotrichum thermophile is cultivated 12 days in nutrient solution, then in cold
Freeze in centrifuge and centrifuge 8 minutes, collect upper strata enzyme clear liquid；
2）By the dry fragrant plant mentioned in ancient texts grinds of 30 parts by weight, 200 mesh sieves are crossed, add 100 parts by weight steps 1）Gained enzyme clear liquid mixes, 70
Place 2 days at DEG C, then filter, the water of 30 parts by weight is added after residue washing 3 times, boil to sticky, then add 30 parts by weight
Water dilutes, and 10 parts by weight of chilled rear addition, the hydrogen peroxide of volumetric concentration 30% boil, and keep boil condition 10 minutes, take out
Filter, produces carragheen, and its recovery rate is 39%, compared with traditional soda（10%-20%）Significantly improve.
The foregoing is only presently preferred embodiments of the present invention, all equivalent changes done according to scope of the present invention patent with
Modification, it should all belong to the covering scope of the present invention.
Claims (3)
1. a kind of method that enzyme liquid using sporotrichum thermophile extracts fragrant plant mentioned in ancient texts carragheen, it is characterised in that：Comprise the following steps：
1）Sporotrichum thermophile is cultivated 10-12 days in nutrient solution, then centrifuges 5-10 minutes in refrigerated centrifuge, in collection
Layer enzyme clear liquid；
2）By dry fragrant plant mentioned in ancient texts grinds, 100-200 mesh sieves are crossed, add step 1）Gained enzyme clear liquid is mixed, and 2- is placed at 50-70 DEG C
5 days, then filter, the water with the weight such as dry fragrant plant mentioned in ancient texts added after residue washing 3-5 times, boil to sticky, then add the dilution of equivalent water,
The hydrogen peroxide for adding volumetric concentration 30% after chilled boils, and is kept for boil condition 3-10 minutes, filters, produces carragheen.
2. the method that the enzyme liquid according to claim 1 using sporotrichum thermophile extracts fragrant plant mentioned in ancient texts carragheen, it is characterised in that：
Step 1）Described in nutrient solution raw material composition be calculated as by weight：Sucrose 1-3 parts, (NH4)2SO40.1-0.3 parts, KH2PO4
0.1-0.3 parts, MgSO4•7H2O 0.01-0.05 parts, CaCl20.01-0.05 parts, peptone 0.1-0.5 parts, 1M urea 0.1-
0.5 part, Citric Acid Mono 0.1-0.5 parts, Na2HPO4•12H2O 0.5-1 parts, distilled water 80-100 parts.
3. the method that the enzyme liquid according to claim 1 using sporotrichum thermophile extracts fragrant plant mentioned in ancient texts carragheen, it is characterised in that：
Step 2）The dosage of each raw material includes by weight：Dry fragrant plant mentioned in ancient texts 10-30 parts, 100 parts of enzyme clear liquid, hydrogen peroxide 5-10 parts.
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109007657A (en) * | 2018-05-14 | 2018-12-18 | 萃奥密公司 | Seawood meal and preparation method thereof |
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| CN105622782A (en) * | 2016-01-15 | 2016-06-01 | 集美大学 | Preparation method for extracting gracilaria agar by replacing alkaline process with enzymatic method |
| CN105985453A (en) * | 2016-07-26 | 2016-10-05 | 绿新（福建）食品有限公司 | Method for extracting carrageenan by using enzyme process instead of alkaline process |
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- 2017-11-22 CN CN201711177059.8A patent/CN107746436A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105622782A (en) * | 2016-01-15 | 2016-06-01 | 集美大学 | Preparation method for extracting gracilaria agar by replacing alkaline process with enzymatic method |
| CN105985453A (en) * | 2016-07-26 | 2016-10-05 | 绿新（福建）食品有限公司 | Method for extracting carrageenan by using enzyme process instead of alkaline process |
Non-Patent Citations (6)
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| 叶勤主编: "《现代生物技术原理及其应用》", 31 August 2003, 中国轻工业出版社 * |
| 张辉等: "一株嗜热纤维素分解菌液体发酵条件初步研究", 《天津农业科学》 * |
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| 赵素芬主编: "《海藻与海藻栽培学》", 30 September 2012, 国防工业出版社 * |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN109007657A (en) * | 2018-05-14 | 2018-12-18 | 萃奥密公司 | Seawood meal and preparation method thereof |
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