CN107714904B - Traditional Chinese medicine composition for improving reproductive performance of breeding male donkeys - Google Patents
Traditional Chinese medicine composition for improving reproductive performance of breeding male donkeys Download PDFInfo
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Abstract
The invention relates to a traditional Chinese medicine composition for improving reproductive performance of male donkeys. A traditional Chinese medicine composition for improving reproductive performance of breeding donkey comprises the following components in parts by weight: herba Epimedii, radix rehmanniae Preparata, semen Cuscutae, radix astragali, cortex Cinnamomi, rhizoma anemarrhenae, and Actinolitum. According to the invention, through a large amount of experimental research on the reproductive performance of the breeding donkey, according to the main symptoms of the breeding male donkey in the current donkey breeding industry, the traditional Chinese medicine composition mainly containing epimedium herb is screened and obtained by combining the bias of the donkey and the dialectical medication mechanism of a veterinarian, so that the low success rate and the kidney deficiency caused by long-term frequent use in the breeding process can be prevented; the number of healthy and alive sperms per unit volume can be greatly increased, and the service life can be greatly prolonged; and the safety is good, the donkey is harmless to the male, and the clinical application prospect is good.
Description
Technical Field
The invention relates to a traditional Chinese medicine composition for improving reproductive performance of breeding donkey, and belongs to the technical field of veterinary medicines.
Background
Donkeys, mammalia, order of perissodactyla, equine, genus equi, equines and donkeys belong to the genus equi, also known as donkeys, but of different species, they have a common origin and are smaller than both horses and zebras but share a number of common characteristics with equines: the third developed toe with hoof, all the remaining toes had degenerated.
The ass meat has the health functions of replenishing blood, benefiting vital energy, etc. The wind eating donkey meat is also rising in Guangdong, Guangxi, Shaanxi, Beijing, Tianjin, Hebei, Shandong, etc. The famous snack is baoding spiced donkey meat, which is produced in Shandong Dezhou donkey, has flexible and thick cortex, can be used for tanning, has medicinal value and is a raw material of rare Chinese medicine 'donkey-hide gelatin'.
At present, the development of donkey product industry is greatly limited by the limitation of donkey reproductive capacity. Researches show that the poor semen quality of the male donkey directly causes the conception rate of the female donkey to be reduced, and the production performance of female animals is influenced. Therefore, the semen quality of the breeding male donkey is improved, the utilization efficiency of the breeding male donkey is improved, the feeding and management cost is saved, the donkey industry development is promoted, and the importance is increased.
The traditional Chinese veterinary medicine considers that reproductive dysfunction of male livestock such as sterility or shortness of birth is usually caused by deficiency of kidney essence and deficiency of qi and blood, and the treatment principle of tonifying the deficiency is mainly to warm and tonify kidney yang, nourish yin, promote the production of body fluid, tonify qi and replenish blood. A plurality of research results show that the traditional Chinese medicine can promote the animal production performance; can promote the development of immune organs and the proliferation of immune cells and enhance the immunity of organisms; can observe the adaptability and tolerance of the organism at any time, so that the traditional Chinese medicine has higher safety when being applied in animal husbandry and veterinary clinic. In the donkey breeding production process, the breeding male donkey fecundity directly influences the production level and the economic benefit. The fertility of the breeding male donkey mainly reflects whether the male donkey can produce semen with good quality and has healthy and vigorous sexual behaviors. Including physiological functions of reproductive organs, normal sexual desire, mating ability, mating load, conception rate of mating female animals, and reproductive and utilization period.
Compared with other animals, donkeys like to live in dry and warm areas, are afraid of cold but are hungry and thirst resistant, and some individuals can eat no food for several days. The donkey feed has strong digestion capability on crude fiber, is resistant to coarse feeding, rarely suffers from digestive tract diseases, but the health and the production performance of the donkey are seriously influenced along with the change of a large-scale and intensive feeding mode in recent years, so that the rapid development of the donkey breeding industry is hindered. Because the donkey belongs to the equus and is a herbivorous animal, the related researches on prevention and control of diseases and promotion of production performance by adopting Chinese herbal medicines are not reported at present according to the change of donkey breeding and the combination of physiological characteristics and Chinese medicine theory of the donkey.
Disclosure of Invention
Aiming at the defects of the prior art, the invention provides a traditional Chinese medicine composition for improving the reproductive performance of breeding male donkeys.
The technical scheme of the invention is as follows:
a traditional Chinese medicine composition for improving reproductive performance of breeding donkey comprises the following components in parts by weight:
55-65 parts of epimedium, 85-95 parts of prepared rehmannia root, 55-65 parts of dodder, 55-65 parts of astragalus root, 25-35 parts of cinnamon, 25-35 parts of rhizoma anemarrhenae and 25-35 parts of actinolite.
According to the preferable traditional Chinese medicine composition for improving the reproductive performance of the breeding donkey, the epimedium herb is prepared from the following components in parts by weight: 55 parts, 56 parts, 57 parts, 58 parts, 59 parts, 60 parts, 61 parts, 62 parts, 63 parts, 64 parts or 65 parts.
According to the preferable selection of the invention, in the traditional Chinese medicine composition for improving the reproductive performance of the breeding donkey, the prepared rehmannia root comprises the following components in parts by weight: 85 parts, 86 parts, 87 parts, 88 parts, 89 parts, 90 parts, 91 parts, 92 parts, 93 parts, 94 parts or 95 parts.
According to the preferable traditional Chinese medicine composition for improving the reproductive performance of the breeding donkey, the semen cuscutae comprises the following components in parts by weight: 55 parts, 56 parts, 57 parts, 58 parts, 59 parts, 60 parts, 61 parts, 62 parts, 63 parts, 64 parts or 65 parts.
According to the preferable selection of the invention, in the traditional Chinese medicine composition for improving the reproductive performance of the breeding donkey, the astragalus membranaceus comprises the following components in parts by weight: 55 parts, 56 parts, 57 parts, 58 parts, 59 parts, 60 parts, 61 parts, 62 parts, 63 parts, 64 parts or 65 parts.
According to the preferable selection of the invention, in the traditional Chinese medicine composition for improving the reproductive performance of the breeding donkey, the cinnamon comprises the following components in parts by weight: 25 parts, 26 parts, 27 parts, 28 parts, 29 parts, 30 parts, 31 parts, 32 parts, 33 parts, 34 parts, or 35 parts.
According to the preferable selection of the invention, in the traditional Chinese medicine composition for improving the reproductive performance of the breeding donkey, the rhizoma anemarrhenae comprises the following components in parts by weight: 25 parts, 26 parts, 27 parts, 28 parts, 29 parts, 30 parts, 31 parts, 32 parts, 33 parts, 34 parts, or 35 parts.
According to the preferable traditional Chinese medicine composition for improving the reproductive performance of the breeding donkey, the actinolite comprises the following components in parts by weight: 25 parts, 26 parts, 27 parts, 28 parts, 29 parts, 30 parts, 31 parts, 32 parts, 33 parts, 34 parts, or 35 parts.
According to the invention, the traditional Chinese medicine composition comprises the following components in parts by weight:
60 parts of epimedium, 90 parts of prepared rhizome of rehmannia, 60 parts of dodder, 60 parts of astragalus, 30 parts of cinnamon, 30 parts of rhizoma anemarrhenae and 30 parts of actinolite.
The application of the traditional Chinese medicine composition for improving the reproductive performance of the breeding male donkey in preparing a medicine for improving the reproductive performance of the breeding male donkey.
The application is that the medicament dosage form is oral liquid.
A preparation method of a traditional Chinese medicine oral liquid for improving reproductive performance of breeding donkey comprises the following steps:
(1) mixing the raw material medicines in the traditional Chinese medicine composition for improving the reproductive performance of the breeding donkey with water according to the mass ratio of 1 (20-30), carrying out water bath extraction for 2-3 times, and combining the extracting solutions to prepare an initial extracting solution;
(2) mixing the dregs extracted in the water bath in the step (1) with an ethanol solution according to the mass ratio of 1 (20-30), performing ultrasonic extraction for 40-50 min under the conditions of ultrasonic power of 250-350W and temperature of 55-65 ℃, performing ultrasonic extraction for 2-3 times, and combining the extracting solutions to prepare an ultrasonic extracting solution;
(3) and (3) mixing the primary extraction liquid prepared in the step (1) and the ultrasonic extraction liquid prepared in the step (2), and concentrating to prepare the traditional Chinese medicine oral liquid.
According to the invention, in the step (1), the water bath extraction conditions are as follows: extracting in water bath at 85-95 ℃ for 1.8-2.5 hours.
According to the present invention, in the step (2), the ethanol solution is 60% ethanol solution by volume concentration.
According to the invention, in the step (3), the negative pressure rotary evaporator is adopted, the temperature is 90 ℃, and the rotating speed is 20 r/min.
Principle of forming a square
According to the compatibility principle of monarch, minister, assistant and guide of the traditional Chinese medicine formula, seven traditional Chinese medicines such as epimedium, prepared rhizome of rehmannia, dodder seed and the like are combined to improve the reproductive performance of the male donkey. The method comprises the following steps:
the prepared rehmannia root is sweet and slightly warm in taste, has the effects of nourishing yin, enriching blood, replenishing vital essence and replenishing marrow, and enters heart, kidney and liver channels; the epimedium herb is pungent in flavor and warm in property, enters kidney channels, has the effects of tonifying kidney yang, strengthening muscles and bones and dispelling wind-damp, and is used as a monarch drug when the two drugs are combined;
the dodder is sweet and warm in taste, enters kidney and liver channels, has the effects of nourishing liver and kidney, improving eyesight and benefiting essence, the astragalus membranaceus is sweet and warm in taste, enters lung and spleen channels, has the functions of tonifying qi, strengthening exterior, inducing diuresis and relieving swelling, the actinolite is salty in taste, warm in nature, enters kidney channels, has the functions of warming and invigorating vital gate, replenishing essence and tonifying yang, and the three medicines are used together as ministerial medicines;
cinnamon is pungent and sweet in flavor and large in nature and heat in nature, enters liver, kidney and spleen channels, has the effects of warming middle-jiao and removing cold, tonifying fire and tonifying yang, and rhizoma anemarrhenae is bitter and cold in flavor, enters kidney, stomach and lung channels, has the effects of clearing heat and purging fire, moistening dryness and nourishing yin, and is used as an adjuvant drug.
The medicines are used together to tonify the kidney, strengthen yang and improve the reproductive capacity of organisms. Donkey is different from horse in disease resistance, clinical symptom expression and response to drugs, etc. because of its own biological characteristics. For example, donkey prefers a warm and dry environment, and is very easily attacked by wind-cold-dampness, and when some diseases are suffered from pathogenic factors such as wind-cold-dampness, the clinical symptoms are more moderate than those of horse, even the external symptoms are not manifested, and subclinical symptoms are manifested. The traditional Chinese medicine and the prescription have more prominent effect on solving the constitution of the subclinical symptom. The traditional Chinese medicine prescription screened by the inventor has obvious curative effect on reproductive dysfunction of the male donkey caused by wind-cold-dampness evil.
Advantageous effects
1. According to the invention, through a large amount of experimental research on the reproductive performance of the breeding donkey, according to the main symptoms of the breeding male donkey in the current donkey breeding industry, the traditional Chinese medicine composition mainly containing epimedium herb is screened and obtained by combining the bias of the donkey and the dialectical medication mechanism of a veterinarian, so that the low success rate and the kidney deficiency caused by long-term frequent use in the breeding process can be prevented; the number of healthy and alive sperms per unit volume can be greatly increased, and the service life can be greatly prolonged; and the safety is good, the donkey is harmless to the male, and the clinical application prospect is good.
2. The polysaccharide and the flavone in the raw material medicines are sequentially extracted by performing water bath extraction and ultrasonic extraction on the raw material medicines in the traditional Chinese medicine composition, so that the active ingredients in the raw material medicines are retained to the greatest extent.
Detailed Description
The technical solution of the present invention is further described with reference to the following examples, but the scope of the present invention is not limited thereto.
Example 1
A traditional Chinese medicine composition for improving reproductive performance of breeding donkey comprises the following components in parts by weight:
60 parts of epimedium, 90 parts of prepared rhizome of rehmannia, 60 parts of dodder, 60 parts of astragalus, 30 parts of cinnamon, 30 parts of rhizoma anemarrhenae and 30 parts of actinolite.
Optimization of extraction process of traditional Chinese medicine composition with epimedium as main drug
The extraction method comprises the following steps: water bath extraction (polysaccharides), ultrasonic ethanol extraction (flavones).
1) Polysaccharide extraction: according to the pre-test results and literature data, 4 single factors influencing polysaccharide extraction are determined and considered: the feed-liquid ratio, the extraction temperature, the extraction time and the extraction frequency are set to 5 levels for each single factor. Controlling single variable, designing single-factor experiment, and extracting in water bath according to different experimental conditions. And (3) performing constant volume on 5 groups of pure liquid medicines obtained in each single-factor experiment in 200ml volumetric flasks, respectively taking 2ml of the pure liquid medicines to dilute 50 times to 100ml of the pure liquid medicines to perform constant volume, finally measuring the OD value at 490nm by using an ultraviolet spectrophotometer, calculating and extracting the polysaccharide content according to a set glucose standard curve, processing the obtained data, and obtaining the optimal single-factor extraction condition according to the peak value. The SPSS software is used for designing orthogonal experiments to obtain 9 groups of orthogonal experiments with 4 factors and 3 levels, the medicine is prepared and the polysaccharide is extracted according to different conditions of each group of experiments, and the obtained data is analyzed by the SPSS orthogonal design software, so that the optimal extraction process is realized.
The best extraction process results are as follows:
in Table 1, the ratio of A material to liquid, the extraction time of B (error), the extraction temperature of C, and the extraction frequency of D;
TABLE 1
a.R Fang ═ 994 (adjustment R Fang ═ 997)
From the above table, D > C > A > B, and therefore, the extraction time of B was selected as an error, and the P value D <0.05< C < A, indicating that the number of extractions was the most significant. The optimal extraction process is that the material-liquid ratio is 1: 25. the extraction time is 2h, the extraction temperature is 90 ℃, and the extraction times are 2 times.
2) And (3) flavone extraction: according to the pre-test results and literature data, 5 single factors influencing the extraction of flavone are determined: the extraction time, the extraction temperature, the material-liquid ratio, the ethanol concentration and the ultrasonic power are set to 6 levels for each single factor except the power, and the ultrasonic power is 5 levels. Preparing ethanol with different concentrations according to single factors and experimental requirements for later use. Then pouring the standby medicine into a conical flask, controlling single variable, designing single-factor experiment, setting an ultrasonic instrument according to different experimental conditions, performing ultrasonic alcohol extraction on flavone, filtering by a Buchner funnel to obtain pure liquid medicine, and drying and storing the medicine dregs in a drying box. Accurately weighing 6 groups of pure liquid medicines obtained in each single-factor experiment in a measuring cylinder, respectively taking 1ml, dripping into a 25ml volumetric flask, simultaneously adding 1ml of 5% sodium nitrite, fully shaking, adding 1ml of 10% aluminum nitrate after 6min, fully shaking, adding 5ml of 4% sodium hydroxide after 6min, directly metering the volume by using 70% ethanol, measuring an OD value at 510nm of an ultraviolet wind spectrophotometer for 15min, calculating the content of extracted flavone according to a set flavone standard curve, processing the obtained data, and obtaining the optimal single-factor extraction condition according to a peak value. Designing orthogonal experiments through SPSS software to obtain 16 groups of orthogonal experiments with 5 factors and 4 levels, preparing the orthogonal experiments according to different experimental conditions of each group of experiments, and extracting flavone. Obtaining the final data, and obtaining the optimal technological conditions for flavone extraction through SPSS software analysis.
The best extraction process results are as follows:
in Table 2, the extraction time A, the material-to-liquid ratio B, the ultrasonic power C (error), the extraction temperature D, and the ethanol concentration E;
TABLE 2
a.R Fang ═ 994 (adjustment R Fang ═ 997)
From the above table, it can be seen that B > D > E > A > C, so the C power is an error, and the P value B <0.05< D < E < A, the most significant ratio of the material to the liquid is obtained. The optimal extraction process is that the time is 45min, and the material-liquid ratio is 1: 25. the ultrasonic power is 300W, the ethanol concentration is 60 percent, and the extraction temperature is 60 ℃.
Therefore, the optimal process conditions for preparing the oral liquid from the important composition are as follows:
(1) mixing the raw materials in the traditional Chinese medicine composition for improving the reproductive performance of the breeding donkey with water according to the mass ratio of 1:25, extracting for 2 hours in a water bath at 90 ℃, extracting for 2 times in the water bath, and combining the extracting solutions to prepare an initial extracting solution;
(2) mixing the residues extracted in the water bath in the step (1) with 60% ethanol solution in a mass ratio of 1:25, performing ultrasonic extraction for 45min at an ultrasonic power of 300W and a temperature of 60 ℃, performing ultrasonic extraction for 2 times, and mixing the extractive solutions to obtain an ultrasonic extractive solution;
(3) and (3) mixing the primary extraction liquid prepared in the step (1) and the ultrasonic extraction liquid prepared in the step (2), and concentrating to prepare the traditional Chinese medicine oral liquid.
According to the invention, in the step (3), the negative pressure rotary evaporator is adopted, the temperature is 90 ℃, and the rotating speed is 20 r/min.
Example 2
A traditional Chinese medicine composition for improving reproductive performance of breeding donkey comprises the following components in parts by weight:
55 parts of epimedium, 95 parts of prepared rhizome of rehmannia, 55 parts of dodder, 65 parts of astragalus root, 25 parts of cinnamon, 35 parts of rhizoma anemarrhenae and 25 parts of actinolite.
The preparation method of the traditional Chinese medicine decoction for improving the reproductive performance of the breeding donkey is the same as that in example 1.
Example 3
A traditional Chinese medicine composition for improving reproductive performance of breeding donkey comprises the following components in parts by weight:
65 parts of epimedium, 85 parts of prepared rhizome of rehmannia, 65 parts of dodder, 55 parts of astragalus root, 35 parts of cinnamon, 25 parts of rhizoma anemarrhenae and 35 parts of actinolite.
The preparation method of the traditional Chinese medicine decoction for improving the reproductive performance of the breeding donkey is the same as that in example 1.
Example 4
A traditional Chinese medicine composition for improving reproductive performance of breeding donkey comprises the following components in parts by weight:
62 parts of epimedium, 92 parts of prepared rhizome of rehmannia, 62 parts of dodder, 62 parts of astragalus root, 32 parts of cinnamon, 32 parts of rhizoma anemarrhenae and 32 parts of actinolite.
The preparation method of the traditional Chinese medicine decoction for improving the reproductive performance of the breeding donkey is the same as that in example 1.
Comparative example 1
62 parts of cistanche, 92 parts of prepared rhizome of rehmannia, 62 parts of dodder, 62 parts of astragalus, 32 parts of cinnamon, 32 parts of rhizoma anemarrhenae and 32 parts of actinolite.
Comparative example 2
62 parts of fructus psoraleae, 92 parts of prepared rhizome of rehmannia, 62 parts of semen cuscutae, 62 parts of astragalus mongholicus, 32 parts of cinnamon, 32 parts of rhizoma anemarrhenae and 32 parts of actinolite.
Comparative example 3
An oral liquid prepared as in example 1 except that the preparation steps were as follows:
(1) mixing the raw materials in the traditional Chinese medicine composition for improving the reproductive performance of the breeding donkey with water according to the mass ratio of 1:25, extracting for 2 hours in a water bath at 90 ℃, extracting for 2 times in the water bath, and combining the extracting solutions to prepare an initial extracting solution;
(2) mixing the residues extracted in the water bath in the step (1) with water according to a mass ratio of 1:25, performing ultrasonic extraction for 45min at an ultrasonic power of 300W and a temperature of 60 ℃, performing ultrasonic extraction for 2 times, and mixing the extracting solutions to obtain an ultrasonic extracting solution;
(3) and (3) mixing the primary extraction liquid prepared in the step (1) and the ultrasonic extraction liquid prepared in the step (2), and concentrating to prepare the traditional Chinese medicine oral liquid.
Examples of the experiments
Weighing the medicines used in the test, wherein each donkey in the high-dose group is administrated with 500g every day, each donkey in the low-dose group is administrated with 300g every day, and after the semen collection of the male donkey used in the test is completed every day, the medicines used in the test and the donkey feed are fully and uniformly mixed, and then the male donkey is fed in a material mixing manner.
Examples 1, 2, 3, 4 and comparative examples 1, 2, 3 were tested and the data analyzed under the same feeding and testing conditions, respectively, and the statistics are shown in table 3:
TABLE 3
Examples of clinical applications
Experiment on influence of traditional Chinese medicine prescription on ejaculatory amount, sperm motility and sperm density of stud donkey
The high dose group is administered with 500g per head donkey per day, and the low dose group is administered with 300g per head donkey per day.
The experimental results of influences of the ejaculatory amount, the sperm motility and the sperm density of the stud donkey are shown in the table 4. the experimental data in the table 4 are observed, for example, the sperm motility range of the stud donkey sperm in the low-dose group 9 before drug feeding is 0.61-0.81, the average sperm density is 2.01, the average ejaculatory amount is 60.7m L, the sperm motility range after drug feeding is 0.67-0.85, the average sperm density is 2.32, and the average ejaculatory amount is 75.3m L.
TABLE 4 influence of the Chinese medicinal formulation on semen quality of stud donkey
Test of influence of Chinese medicinal preparation on sperm teratogenesis rate, ejaculation time and sexual desire score
Weighing the medicines used in the test, wherein each donkey in the high-dose group is administrated with 500g every day, each donkey in the low-dose group is administrated with 300g every day, and after the semen collection of the male donkey used in the test is completed every day, the medicines used in the test and the donkey feed are fully and uniformly mixed, and then the male donkey is fed in a material mixing manner.
The results of the tests on the influence of the Chinese medicinal formula on the sperm abnormality rate, ejaculation time and sexual desire score of the breeding male donkey are shown in Table 5. The average sperm abnormality rate of the male donkeys in the 7 th group is 5.70% before drug feeding and 5.14% after drug feeding, the average ejaculation time of the male donkeys before drug feeding is 49.58s and the average ejaculation time of the male donkeys after drug feeding is 60.55s, and the sexual desire score before drug feeding is 1.60 after drug feeding, so that the sperm abnormality rate, the ejaculation time and the sexual desire score of the male donkeys after drug feeding are obviously improved. Meanwhile, the semen of other groups of donkey can be improved to different degrees by observing other groups of data on the table, so that the traditional Chinese medicine prescription participating in the test is beneficial to improving the teratospermia rate of the sperm of the breeding male donkey, the ejaculation time and the sexual desire score of the breeding male donkey, and has obvious effect on improving the physique of the breeding male donkey.
TABLE 5 influence of the Chinese medicinal formulation on semen quality of breeding male donkey
The average value of each test index of the high and low dose groups is compared with the corresponding data before test
Weighing the medicines used in the test, wherein each donkey in the high-dose group is administrated with 500g every day, each donkey in the low-dose group is administrated with 300g every day, and after the semen collection of the male donkey used in the test is completed every day, the medicines used in the test and the donkey feed are fully and uniformly mixed, and then the male donkey is fed in a material mixing manner.
The corresponding data of the experimental indexes of the high-low dose group are shown in a table 6, the sperm motility of the stud donkey in the low-dose group after medicine feeding is 0.67-0.84, the average sperm density is 2.11, the average ejaculation amount is 72.81m L, the sperm motility of the stud donkey in the high-dose group after medicine feeding is 0.66-0.85, the average sperm density is 2.46 and the average ejaculation amount is 73.37m L.
Table 6 average values of the detection indexes of the low-dose group and the high-dose group of the breeding donkey.
The conditions of the same-proportion increase of each detection index of the low-dose group and the high-dose group of the test breeding donkey
Weighing the medicines used in the test, wherein each donkey in the high-dose group is administrated with 500g every day, each donkey in the low-dose group is administrated with 300g every day, and after the semen collection of the male donkey used in the test is completed every day, the medicines used in the test and the donkey feed are fully and uniformly mixed, and then the male donkey is fed in a material mixing manner.
As can be seen from Table 7, the upper limit of sperm motility of the stud donkey in the low-dose group after being fed with the drug is increased by 1.20 percent, the lower limit of sperm motility is increased by 4.69 percent, the sperm density is increased by 8.76 percent, and the ejaculation amount is increased by 15.04 percent; after the male donkey is fed with the medicine, the sperm motility upper limit is increased by 3.66 percent, the sperm motility lower limit is increased by 8.20 percent, the sperm density is increased by 24.87 percent, the ejaculatory amount is increased by 37.04 percent, and the upper bar chart shows that the effect of the medicine high-dose group used in the test is obviously higher than the test result of the low-dose group, and the high-dose traditional Chinese medicine prescription is more beneficial to improving the semen quality of the male donkey.
TABLE 7 comparably increasing conditions of various detection indexes of low-dose group and high-dose group of experimental breeding donkey
Relationship between age and change of each index before and after breeding donkey test
The breeding donkey is divided into four groups according to the age groups of 4 years, 5 years, 7 years and 8 years, and each group has 5 heads. Daily eating and semen collection are guaranteed, 500g of semen is fed to each donkey after daily semen collection of the male donkey used in the test is completed, the semen is fully mixed with donkey feed, and the male donkey is fed in a material mixing mode.
As can be seen from table 8, the sperm motility of the stud donkeys with the Chinese herbal medicines has the most obvious effect of improving the sperm quality of the stud donkeys at the ages of 4 years and 8 years, while the sperm motility of the sperm of the stud donkeys at the ages of 5 years and 7 years is relatively unobvious. The ejaculatory amount and sperm density of the stud donkey are improved most obviously at the age of 8 years, and the sperm quality is improved most. Therefore, the Chinese herbal medicine prescription used in the experiment is most beneficial to improving the semen quality of the old male donkey.
TABLE 8 influence of age on the sperm data of breeder donkey
Analysis of results
The experimental results show that the ejaculatory amount, the sperm motility, the sperm density and the sexual desire score of the male donkey after being fed with a traditional Chinese medicine formula are improved to different degrees, the sperm abnormality rate is reduced, the ejaculatory amount of the male donkey in a high-dose group and a low-dose group generally begins to increase in the second week after being fed with the traditional Chinese medicine formula, the sperm motility reaches more than 80m L in the second week after being fed with the traditional Chinese medicine formula, the difference is obvious compared with that before being fed with the traditional Chinese medicine formula (P <0.05), the sperm motility increases to a peak in the third week after being fed with the traditional Chinese medicine formula, some experimental donkeys can reach 120-150 m L, the difference is obvious compared with that before being fed with the traditional Chinese medicine formula (P <0.01), the sperm motility of the sperm after being fed with the traditional Chinese medicine formula is increased to 3.66%, the sperm motility of the low-dose group is increased to 1.20%, the sperm motility of the high-dose group is increased to 4.69%, and the sperm motility score of the traditional Chinese medicine formula is improved by the lower limit of the traditional Chinese medicine formula.
Therefore, the experimental traditional Chinese medicine prescription can obviously improve the ejaculatory amount, the sperm motility, the density and the sexual desire score of the male donkey, obviously reduce the sperm aberration rate and improve the reproductive performance of the male donkey, thereby having the effect of exactly improving the semen quality. In addition, the test is analyzed by detection indexes of test rates of different ages, so that the efficacy of the tested donkey at the ages of 4 years and 8 years on the traditional Chinese medicine is more obvious, and the donkey is probably closely related to the development and physiological function decline of the kidney and the spleen. In conclusion, the overall improvement of semen quality starts from week 1 and is significantly improved by week 3, and the libido of the breeding donkey is gradually increased along with the improvement of semen quality.
Claims (15)
1. The traditional Chinese medicine composition for improving reproductive performance of breeding male donkeys is characterized by comprising the following components in parts by weight:
55-65 parts of epimedium, 85-95 parts of prepared rehmannia root, 55-65 parts of dodder, 55-65 parts of astragalus root, 25-35 parts of cinnamon, 25-35 parts of rhizoma anemarrhenae and 25-35 parts of actinolite.
2. The traditional Chinese medicine composition of claim 1, wherein in the traditional Chinese medicine composition for improving reproductive performance of breeding donkey, the epimedium is prepared from the following raw materials in parts by weight: 55 parts, 56 parts, 57 parts, 58 parts, 59 parts, 60 parts, 61 parts, 62 parts, 63 parts, 64 parts or 65 parts.
3. The traditional Chinese medicine composition of claim 1, wherein the traditional Chinese medicine composition for improving reproductive performance of the breeding donkey comprises the following components in parts by weight: 85 parts, 86 parts, 87 parts, 88 parts, 89 parts, 90 parts, 91 parts, 92 parts, 93 parts, 94 parts or 95 parts.
4. The traditional Chinese medicine composition of claim 1, wherein the traditional Chinese medicine composition for improving reproductive performance of the breeding donkey comprises the following semen cuscutae in parts by weight: 55 parts, 56 parts, 57 parts, 58 parts, 59 parts, 60 parts, 61 parts, 62 parts, 63 parts, 64 parts or 65 parts.
5. The traditional Chinese medicine composition of claim 1, wherein the traditional Chinese medicine composition for improving reproductive performance of breeding donkey comprises the following components in parts by weight: 55 parts, 56 parts, 57 parts, 58 parts, 59 parts, 60 parts, 61 parts, 62 parts, 63 parts, 64 parts or 65 parts.
6. The traditional Chinese medicine composition of claim 1, wherein in the traditional Chinese medicine composition for improving reproductive performance of breeding donkey, cinnamon is in parts by weight: 25 parts, 26 parts, 27 parts, 28 parts, 29 parts, 30 parts, 31 parts, 32 parts, 33 parts, 34 parts, or 35 parts.
7. The traditional Chinese medicine composition of claim 1, wherein in the traditional Chinese medicine composition for improving reproductive performance of breeding donkey, the rhizoma anemarrhenae comprises the following components in parts by weight: 25 parts, 26 parts, 27 parts, 28 parts, 29 parts, 30 parts, 31 parts, 32 parts, 33 parts, 34 parts, or 35 parts.
8. The traditional Chinese medicine composition of claim 1, wherein the traditional Chinese medicine composition for improving reproductive performance of breeding donkey comprises the following actinolite in parts by weight: 25 parts, 26 parts, 27 parts, 28 parts, 29 parts, 30 parts, 31 parts, 32 parts, 33 parts, 34 parts, or 35 parts.
9. The traditional Chinese medicine composition of claim 1, wherein the traditional Chinese medicine composition comprises the following components in parts by weight:
60 parts of epimedium, 90 parts of prepared rhizome of rehmannia, 60 parts of dodder, 60 parts of astragalus, 30 parts of cinnamon, 30 parts of rhizoma anemarrhenae and 30 parts of actinolite.
10. The use of the Chinese medicinal composition of claim 1 in the preparation of a medicament for improving reproductive performance of breeding donkey.
11. The use of claim 10, wherein the pharmaceutical dosage form is an oral liquid.
12. A preparation method of a traditional Chinese medicine oral liquid for improving reproductive performance of breeding donkey is characterized by comprising the following steps:
(1) mixing the raw material medicines in the traditional Chinese medicine composition for improving the reproductive performance of the breeding donkey according to any one of claims 1 to 9 with water according to the mass ratio of 1 (20-30), performing water bath extraction for 2-3 times, and combining the extracting solutions to prepare an initial extracting solution;
(2) mixing the dregs extracted in the water bath in the step (1) with an ethanol solution according to the mass ratio of 1 (20-30), performing ultrasonic extraction for 40-50 min under the conditions of ultrasonic power of 250-350W and temperature of 55-65 ℃, performing ultrasonic extraction for 2-3 times, and combining the extracting solutions to prepare an ultrasonic extracting solution;
(3) and (3) mixing the primary extraction liquid prepared in the step (1) and the ultrasonic extraction liquid prepared in the step (2), and concentrating to prepare the traditional Chinese medicine oral liquid.
13. The method of claim 12, wherein in step (1), the water bath extraction conditions are: extracting in water bath at 85-95 ℃ for 1.8-2.5 hours.
14. The method according to claim 12, wherein in the step (2), the ethanol solution is 60% by volume ethanol solution.
15. The method according to claim 12, wherein in the step (3), the temperature of the negative pressure rotary evaporator is 90 ℃ and the rotation speed is 20 rpm.
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