CN107711699B - Prevention method for white spot disease of soft-shelled turtle seedlings - Google Patents

Prevention method for white spot disease of soft-shelled turtle seedlings Download PDF

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Publication number
CN107711699B
CN107711699B CN201711077114.6A CN201711077114A CN107711699B CN 107711699 B CN107711699 B CN 107711699B CN 201711077114 A CN201711077114 A CN 201711077114A CN 107711699 B CN107711699 B CN 107711699B
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water
soft
turtle seedlings
seedlings
partition wall
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CN107711699A (en
Inventor
周洵
俞爱萍
段国庆
张年军
陈钫
胡玉婷
柴继芳
陈冬林
章星明
王晨旭
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Sixian Feihong Sports Culture Development Co., Ltd
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Sixian Feihong Sports Culture Development Co Ltd
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    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01KANIMAL HUSBANDRY; CARE OF BIRDS, FISHES, INSECTS; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K67/00Rearing or breeding animals, not otherwise provided for; New breeds of animals
    • A01K67/02Breeding vertebrates
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61KPREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K35/00Medicinal preparations containing materials or reaction products thereof with undetermined constitution
    • A61K35/56Materials from animals other than mammals
    • A61K35/63Arthropods
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61KPREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18Magnoliophyta (angiosperms)
    • A61K36/185Magnoliopsida (dicotyledons)
    • A61K36/28Asteraceae or Compositae (Aster or Sunflower family), e.g. chamomile, feverfew, yarrow or echinacea
    • A61K36/282Artemisia, e.g. wormwood or sagebrush
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61KPREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18Magnoliophyta (angiosperms)
    • A61K36/185Magnoliopsida (dicotyledons)
    • A61K36/70Polygonaceae (Buckwheat family), e.g. spineflower or dock
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61KPREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18Magnoliophyta (angiosperms)
    • A61K36/185Magnoliopsida (dicotyledons)
    • A61K36/73Rosaceae (Rose family), e.g. strawberry, chokeberry, blackberry, pear or firethorn
    • A61K36/736Prunus, e.g. plum, cherry, peach, apricot or almond
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61KPREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18Magnoliophyta (angiosperms)
    • A61K36/88Liliopsida (monocotyledons)
    • A61K36/888Araceae (Arum family), e.g. caladium, calla lily or skunk cabbage

Abstract

The invention discloses a method for preventing white spot disease of soft-shelled turtle seedlings, which comprises the following steps of 1) building a culture pond; 2) sterilizing the soft-shelled turtle seedlings twice before stocking; 3) changing water after culturing for 15-20 days, observing whether diseased turtle seedlings exist, culturing the sick turtle seedlings for 15 days again, changing water, sterilizing the diseased turtle seedlings, and adding the first medicine for culturing for three days; 4) changing water, disinfecting and adding a second medicine to the diseased turtle seedlings, changing water, disinfecting and adding a third medicine to the diseased turtle seedlings after three days, and 5) fishing the diseased turtle seedlings out after three days, putting the diseased turtle seedlings into a basin, disinfecting and cleaning the bottom of a culture pond; 6) and (5) sterilizing the soft-shelled turtle seedlings for two times again, and repeating the steps. The breeding method is simple and easy to operate, can effectively prevent the turtle seedlings from generating leukoderma, and can provide good living environment for the turtle seedlings.

Description

Prevention method for white spot disease of soft-shelled turtle seedlings
Technical Field
The invention relates to a turtle breeding technology, in particular to a method for preventing white spot disease of turtle seedlings.
Background
The white spot disease of the soft-shelled turtle fry is also one of the diseases with high mortality rate in the white breeding stage of the soft-shelled turtle fry, if the control is not timely, the mortality rate can reach below 30 percent, the disease is mostly caused by the soft-shelled turtle fry being hatched out of shells, temporarily bred and bred improperly, or infected by pathogenic bacteria after the body surface is damaged or the environment is worsened due to reasons such as excessive breeding density, the difference is that the pathogen of the white spot disease is bacteria, the pathogen of the white spot disease is fungi, the pathogenic fungi mainly comprising saprolegnia, cotton mold and white spot disease are more than the closed greenhouse breeding environment without illumination, and white flocculent plaques can be seen when the skirt edge and the neck of the soft-shelled turtle fry are observed underwater. White or light yellow cluster-shaped mycelia can be seen on the neck and the legs of the body surfaces of diseased turtle seedlings when the disease occurs in pond culture outside spring and autumn, the diseased turtle seedlings can move slowly at the beginning and can also eat, and some of the diseased turtle seedlings stop eating after the disease is serious, float on the water surface of a pond corner, lie prone and eat still as above, and finally die in outbreak due to reduced resistance or other diseases.
Disclosure of Invention
In order to solve the problems, the invention provides a culture method capable of preventing the white spot disease of the soft-shelled turtle seedlings.
In order to achieve the aim, the invention is realized by the following technical scheme:
the invention relates to a prevention method of white spot disease of soft-shelled turtle seedlings, which comprises the following steps of 1) building a culture pond, wherein the culture pond is a circular pond, a partition wall is arranged in the middle of the circular pond, a channel with the height of 5-15 cm is arranged between the bottom of the partition wall and the bottom of the circular pond, a motor is arranged on the partition wall and is connected with a rotating shaft, the rotating shaft penetrates through the partition wall, a scraper is fixed on the rotating shaft at the lower end of the partition wall, a brush plate is arranged at the lower end of the scraper, protruding fixing edges are arranged on two sides of the partition wall outside the channel, slots are arranged on the fixing edges, baffles for preventing water in the circular ponds at two sides of the partition wall from mutually circulating are arranged in the slots, a pull rod is arranged in the middle of the upper end of each baffle, a plurality of; 2) sterilizing the soft-shelled turtle seedlings twice before stocking, sterilizing the soft-shelled turtle seedlings for 5 minutes by adopting 2-3% saline water for the first sterilization, cleaning the soft-shelled turtle seedlings in a clean water tank for 1-3 minutes after the sterilization, sterilizing the soft-shelled turtle seedlings for 5 minutes for the second time by adopting 1% saline water, and then putting the soft-shelled turtle seedlings into a breeding tank on one side of a partition wall for breeding after the sterilization; 3) after 15-20 days of cultivation, transferring the turtle seedlings into a cultivation pond on the other side of the partition wall for cultivation, observing the surfaces of the turtle seedlings in the process of transferring the turtle seedlings, cultivating for 15 days without damage to the surfaces of the turtle seedlings, transferring again, pumping water in the cultivation pond on one side of the partition wall of the previously cultivated turtle seedlings under the condition of damage to the surfaces of the turtle seedlings, insolating for 1 day, putting the water in the cultivation pond on one side where the turtle seedlings are cultivated into the water which just submerges the back of the turtle seedlings, adding a first-time liquid medicine into the cultivation pond, and adding the water in the cultivation pond to the water level of normal cultivation after 1 hour; 4) transferring the soft-shelled turtle seedlings to a culture pond on the other side of the partition wall after three days, discharging water into the culture pond until the water level is over the back of the soft-shelled turtle seedlings, adding a liquid medicine into the culture pond for the second time, adding the water in the culture pond to the water level for normal culture after 1 hour, transferring the soft-shelled turtle seedlings again after three days, discharging water into the culture pond where the soft-shelled turtle seedlings are located until the water level is over the back of the soft-shelled turtle seedlings, and adding the liquid medicine for the third time; 5) after three days, fishing the turtle seedlings out and putting the turtle seedlings into a basin, pulling up a baffle, adding water into the culture pond to a normal culture water level, scattering potassium permanganate into the water for disinfection for 2-3 hours, starting a scraper to clean the bottom of the culture pond, pumping the water away in the process of rotation of the scraper, and recovering the scraper and the baffle after the water is drained; 6) and (5) sterilizing the soft-shelled turtle seedlings twice, and repeating the steps.
The invention is further improved in that: and a plurality of fixing columns for fixing pull ropes are arranged on two sides of the upper end of the partition wall.
The invention is further improved in that: the lower end of the baffle is inserted into a groove at the bottom of the round pool.
The invention is further improved in that: the wall of the bottom of the round pool on two sides of the partition wall is provided with a water pumping hole, a water pumping pipe connected with a water pumping pump is fixed in the water pumping hole, and water discharging pipes for discharging water on two sides of the partition wall of the round pool are fixed on two sides of the partition wall.
The invention is further improved in that: the liquid medicine is prepared by decocting 10% of folium artemisiae argyi, 10% of rhizoma acori graminei, 40% of gallnut, 20% of rumex japonicus and 20% of dark plum.
The invention is further improved in that: the amount of the liquid medicine added in the third time in the step 3 is respectively 40 g for the first time, 30 g for the second time and 25 g for the third time in each cubic meter of water body.
The invention is further improved in that: the water temperature is controlled at 28-30 ℃ in the culture process.
The invention has the beneficial effects that: the bottom of the breeding pond can be automatically cleaned by controlling the motor, food residues and excreted dirt are prevented from being rotted in a water body for a long time to breed germs, the soft-shelled turtle seedlings are disinfected before stocking, germs are prevented from being attached to the body surfaces of the soft-shelled turtle seedlings and being brought into the breeding pond, and the breeding pond for the sick soft-shelled turtle seedlings is disinfected by adding medicine for three times in the breeding process.
The breeding method is simple and easy to operate, can effectively prevent the turtle seedlings from generating leukoderma, and can provide good living environment for the turtle seedlings.
Drawings
FIG. 1 is a schematic view of a culture pond.
Wherein: 1-partition wall, 2-motor, 3-scraper and 4-baffle.
Detailed Description
For the purpose of enhancing an understanding of the present invention, the present invention will be described in further detail with reference to the accompanying drawings and examples, which are provided for the purpose of illustration only and are not intended to limit the scope of the present invention.
As shown in figure 1, the invention relates to a method for preventing white spot disease of soft-shelled turtle seedlings, which comprises 1) building a culture pond, wherein the culture pond is a circular pond, a partition wall 1 is arranged in the middle of the circular pool, a passage with the height of 5-15 cm is arranged between the bottom of the partition wall 1 and the bottom of the circular pool, the partition wall 1 is provided with a motor 2, the motor 2 is connected with a rotating shaft, the rotating shaft penetrates through the partition wall 1, a scraping plate 3 is fixed on a rotating shaft at the lower end of the partition wall 1, a brush board is arranged at the lower end of the scraping plate 3, two sides of the partition wall 1 outside the channel are provided with convex fixing edges, the fixing edges are provided with slots, baffle plates 4 for preventing water in the circular tanks at the two sides of the partition wall 1 from mutually circulating are arranged in the slots, a pull rod is arranged in the middle of the upper end of the baffle 4, a plurality of holes are symmetrically arranged on the baffle 4 on two sides of the pull rod, and a plurality of pull ropes are fixed on the baffle 4 through the holes; 2) sterilizing the soft-shelled turtle seedlings twice before stocking, sterilizing the soft-shelled turtle seedlings for 5 minutes by adopting 2-3% saline water for the first sterilization, cleaning the soft-shelled turtle seedlings in a clean water tank for 1-3 minutes after the sterilization, sterilizing the soft-shelled turtle seedlings for 5 minutes for the second time by adopting 1% saline water, and then putting the soft-shelled turtle seedlings into a breeding tank on one side of a partition wall for breeding after the sterilization; 3) after 15-20 days of cultivation, transferring the turtle seedlings into a cultivation pond on the other side of the partition wall for cultivation, observing the surfaces of the turtle seedlings in the process of transferring the turtle seedlings, cultivating for 15 days without damage to the surfaces of the turtle seedlings, transferring again, pumping water in the cultivation pond on one side of the partition wall of the previously cultivated turtle seedlings under the condition of damage to the surfaces of the turtle seedlings, insolating for 1 day, putting the water in the cultivation pond on one side where the turtle seedlings are cultivated into the water which just submerges the back of the turtle seedlings, adding a first-time liquid medicine into the cultivation pond, and adding the water in the cultivation pond to the water level of normal cultivation after 1 hour; 4) transferring the soft-shelled turtle seedlings to a culture pond on the other side of the partition wall after three days, discharging water into the culture pond until the water level is over the back of the soft-shelled turtle seedlings, adding a liquid medicine into the culture pond for the second time, adding the water in the culture pond to the water level for normal culture after 1 hour, transferring the soft-shelled turtle seedlings again after three days, discharging water into the culture pond where the soft-shelled turtle seedlings are located until the water level is over the back of the soft-shelled turtle seedlings, and adding the liquid medicine for the third time; 5) after three days, fishing the turtle seedlings out and putting the turtle seedlings into a basin, pulling up a baffle, adding water into the culture pond to a normal culture water level, scattering potassium permanganate into the water for disinfection for 2-3 hours, starting a scraper to clean the bottom of the culture pond, pumping the water away in the process of rotation of the scraper, and recovering the scraper and the baffle after the water is drained; 6) sterilizing the young soft-shelled turtle twice, repeating the above steps, arranging a plurality of fixing columns for fixing the pull ropes on two sides of the upper end of the partition wall, inserting the lower end of the baffle plate into the groove at the bottom of the round pool, the walls of the bottom parts of the round tanks at the two sides of the partition wall are provided with water pumping holes, water pumping pipes connected with water pumping pumps are fixed in the water pumping holes, the water discharge pipes for discharging water on two sides of the partition wall of the circular pool are fixed on two sides of the partition wall, the liquid medicine is prepared by decocting 10% of folium artemisiae argyi, 10% of rhizoma acori graminei, 40% of gallnut, 20% of radix rumicis japonicus and 20% of dark plum, the dosage of the liquid medicine added in the step 3 is respectively 40 g for the first time, 30 g for the second time and 25 g for the third time in each cubic meter of water, the water temperature in the culture process is controlled to be 28-30 ℃, the water temperature of 28-30 ℃ can keep the strongest activity of the soft-shelled turtle seedling, and meanwhile, the germ reproduction can be inhibited.
The breeding method is simple and easy to operate, can effectively prevent the turtle seedlings from generating leukoderma, and can provide good living environment for the turtle seedlings.

Claims (4)

1. A method for preventing white spot disease of soft-shelled turtle seedlings is characterized by comprising the following steps: 1) building a culture pond which is a circular pond, wherein a partition wall (1) is arranged in the middle of the circular pond, a channel with the height of 5-15 cm is arranged between the bottom of the partition wall (1) and the bottom of the circular pond, a motor (2) is arranged on the partition wall (1), the motor (2) is connected with a rotating shaft, the rotating shaft penetrates through the partition wall (1), a scraper (3) is fixed on the rotating shaft at the lower end of the partition wall (1), a brush board is arranged at the lower end of the scraper (3), convex fixing edges are arranged at two sides of the partition wall (1) outside the channel, inserting grooves are arranged on the fixing edges, baffle plates (4) for preventing water in the circular pond at two sides of the partition wall (1) from mutually circulating are arranged in the inserting grooves, and pull rods are arranged in the middle of the upper ends of the baffle plates (4), a plurality of holes are symmetrically formed in the baffle (4) on two sides of the pull rod, and a plurality of pull ropes are fixed on the baffle (4) through the holes; 2) sterilizing the soft-shelled turtle seedlings twice before stocking, sterilizing the soft-shelled turtle seedlings for 5 minutes by adopting 2-3% saline water for the first sterilization, cleaning the soft-shelled turtle seedlings in a clean water tank for 1-3 minutes after the sterilization, sterilizing the soft-shelled turtle seedlings for 5 minutes for the second time by adopting 1% saline water, and then putting the soft-shelled turtle seedlings into a breeding tank on one side of a partition wall for breeding after the sterilization; 3) after 15-20 days of cultivation, transferring the turtle seedlings into a cultivation pond on the other side of the partition wall for cultivation, observing the surfaces of the turtle seedlings in the process of transferring the turtle seedlings, cultivating for 15 days without damage to the surfaces of the turtle seedlings, transferring again, pumping water in the cultivation pond on one side of the partition wall of the previously cultivated turtle seedlings under the condition of damage to the surfaces of the turtle seedlings, insolating for 1 day, putting water which just overflows the back of the turtle seedlings into the cultivation pond on one side where the turtle seedlings are cultivated, adding a first-time liquid medicine into the cultivation pond, and adding the water in the cultivation pond to the water level of normal cultivation after 1 hour; 4) transferring the soft-shelled turtle seedlings to a culture pond on the other side of the partition wall after three days, discharging water into the culture pond until the water level is over the back of the soft-shelled turtle seedlings, adding a liquid medicine into the culture pond for the second time, adding the water in the culture pond to the water level for normal culture after 1 hour, transferring the soft-shelled turtle seedlings again after three days, discharging water into the culture pond where the soft-shelled turtle seedlings are located until the water level is over the back of the soft-shelled turtle seedlings, and adding the liquid medicine for the third time; 5) after three days, fishing the turtle seedlings out and putting the turtle seedlings into a basin, pulling up a baffle, adding water into the culture pond to a normal culture water level, scattering potassium permanganate into the water for disinfection for 2-3 hours, starting a scraper to clean the bottom of the culture pond, pumping the water away in the process of rotation of the scraper, and recovering the scraper and the baffle after the water is drained; 6) sterilizing the soft-shelled turtle seedlings for two times again, and repeating the steps;
the two sides of the upper end of the partition wall are provided with a plurality of fixed columns for fixing the pull ropes, the lower end of the baffle is inserted into the groove at the bottom of the circular pool, the two sides of the partition wall are provided with water pumping holes in the wall at the bottom of the circular pool, water pumping pipes connected with water pumping pumps are fixed in the water pumping holes, and water drainage pipes for preventing water are fixed at the two sides of the partition wall of the circular pool.
2. The method for preventing white spot disease of young soft-shelled turtles according to claim 1, wherein the method comprises the following steps: the liquid medicine is prepared by decocting 10% of folium artemisiae argyi, 10% of rhizoma acori graminei, 40% of gallnut, 20% of radix Rumicis Japonici and 20% of dark plum.
3. The method for preventing white spot disease of young soft-shelled turtles according to claim 1, wherein the method comprises the following steps: the amount of the liquid medicine added in the third time in the step 3 is respectively 40 g for the first time, 30 g for the second time and 25 g for the third time in each cubic meter of water body.
4. The method for preventing white spot disease of young soft-shelled turtles according to claim 1, wherein the method comprises the following steps: the water temperature is controlled at 28-30 ℃ in the culture process.
CN201711077114.6A 2017-11-06 2017-11-06 Prevention method for white spot disease of soft-shelled turtle seedlings Active CN107711699B (en)

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Citations (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101120662B (en) * 2007-08-04 2011-06-22 杭州萧山天福生物科技有限公司 Method for selectively breeding improved variety of China turtle
CN102726327A (en) * 2012-06-20 2012-10-17 中国水产科学研究院东海水产研究所 Method for removing residual cryptocryon irritans from culture pond in pollution-free way
JP2013106565A (en) * 2011-11-21 2013-06-06 Acurusu:Kk Ozone sterilizing apparatus
CN104435595A (en) * 2014-11-06 2015-03-25 占水秀 Medicine for treating white spot disease of turtle
CN205658212U (en) * 2016-06-05 2016-10-26 周京壮 Sea eel culturing device
CN106259180A (en) * 2016-08-15 2017-01-04 王炳付 A kind of cultural method of Trionyx sinensis Wiegmann
CN107006538A (en) * 2017-05-27 2017-08-04 明光市裕阳农业有限公司 A kind of plant environment-friendly type bactericide used for aquiculture and preparation method thereof
CN107027828A (en) * 2017-06-06 2017-08-11 合肥雪立歆农业科技有限公司 A kind of plant environment-friendly type bactericide used for aquiculture and preparation method thereof

Patent Citations (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101120662B (en) * 2007-08-04 2011-06-22 杭州萧山天福生物科技有限公司 Method for selectively breeding improved variety of China turtle
JP2013106565A (en) * 2011-11-21 2013-06-06 Acurusu:Kk Ozone sterilizing apparatus
CN102726327A (en) * 2012-06-20 2012-10-17 中国水产科学研究院东海水产研究所 Method for removing residual cryptocryon irritans from culture pond in pollution-free way
CN104435595A (en) * 2014-11-06 2015-03-25 占水秀 Medicine for treating white spot disease of turtle
CN205658212U (en) * 2016-06-05 2016-10-26 周京壮 Sea eel culturing device
CN106259180A (en) * 2016-08-15 2017-01-04 王炳付 A kind of cultural method of Trionyx sinensis Wiegmann
CN107006538A (en) * 2017-05-27 2017-08-04 明光市裕阳农业有限公司 A kind of plant environment-friendly type bactericide used for aquiculture and preparation method thereof
CN107027828A (en) * 2017-06-06 2017-08-11 合肥雪立歆农业科技有限公司 A kind of plant environment-friendly type bactericide used for aquiculture and preparation method thereof

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