JP2013106565A - Ozone sterilizing apparatus - Google Patents

Abstract

PROBLEM TO BE SOLVED: To promote size-reduction and improve economic efficiency of a sterilizing facility by directly introducing microbubbles of ozone having a specific dissolved concentration into a rearing aquarium for aquatic animals.SOLUTION: Ozone-containing bubbles B are introduced into rearing water W of the rearing aquarium 2 for aquatic animals S. The ozone sterilizing facility is provided with an ozone-generator 3 for generating ozone, a bubble forming unit 4 for forming bubbles B containing ozone generated by the ozone-generation means 3, and a direct introduction unit 5 for introducing the bubbles B formed by the bubble forming unit 4 directly into the rearing water W of the rearing aquarium 2 in which the aquatic animals S are reared. The bubbles B contain microbubbles M having a median diameter L1 of ≥1 μm and ≤500 μm during formation, and the dissolved ozone concentration Z1 in the rearing water W is ≥0.05 mg/l and ≤0.50 mg/l.

Description

The present invention relates to an ozone sterilization apparatus for sterilizing breeding water used for breeding aquatic animals such as seafood, whales and sea turtles.
In the present invention, breeding water is not limited to the water in a ginger (breeding aquarium) used in aquaculture business or restaurants, but is also used for aquarium containers used during transportation of aquatic animals, ornamental fish such as tropical fish, and aquariums. Also includes water in breeding tanks.

Conventionally, a method for purifying fish tank water has been known.
In this purification method, seawater in a fish tank or fresh water to which bromine is added to the same degree as seawater is derived from the tank, ozone gas is injected into the derived tank water, and then the tank water is gas-liquid separated. After removing unreacted ozone, it is passed through a manganese dioxide catalyst filter medium and refluxed to a water tank (see Patent Document 1).

JP-A-4-267829

  The conventional fish tank water purification methods described above remove the unreacted ozone from the circulating pump that pumps seawater in the tank, the ejector that injects ozone gas in the middle of pumping, and the pumped seawater, because dissolved ozone is harmful to fish Therefore, it is inevitable to increase the size of the purification facility.

  In view of such a point, the ozone disinfection apparatus according to the present invention directly disinfects the breeding water by introducing ozone microbubbles having a predetermined degree of dissolution directly into a breeding tank where aquatic animals are raised. An object of the present invention is to provide an ozone sterilization apparatus that enables ozone sterilization that does not hinder the breeding of aquatic animals and that can realize downsizing of a sterilization facility.

In order to achieve the above object, the present invention employs the following technical means.
The ozone sterilization apparatus according to the present invention is an ozone sterilization apparatus that first introduces bubbles B containing ozone into the breeding water W of the breeding aquarium 2 of the aquatic animal S. The ozone sterilization apparatus generates ozone. Generating means 3, bubble generating means 4 for generating bubbles B containing ozone generated by the ozone generating means 3, and breeding aquarium in which aquatic animals S are bred with the bubbles B generated by the bubble generating means 4 And the direct entry means 5 for direct introduction into the breeding water W, the bubbles B include microbubbles M having a central particle size L1 of 1 μm or more and 500 μm or less at the time of generation, and ozone in the breeding water W The solubility Z1 is 0.05 mg / l or more and 0.50 mg / l or less.
The ozone solubility Z1 in the breeding water W is the mass (mg) of ozone contained in one liter (l) of the breeding water W.

Second, the ozone concentration Z2 generated by the ozone generating means 3 is 0.05 mg / l or more and 0.35 mg / l or less.
The ozone concentration Z2 is the mass (mg) of ozone contained in 1 liter (l) of gas containing ozone.

  Thirdly, the bubble B also includes nanobubbles N having a central particle size L2 of 1 nm or more and 500 nm or less at the time of generation.

  Fourth, at least a part of the nanobubbles N is generated by contracting microbubbles M having a central particle size L1 of 1 μm or more and 50 μm or less at the time of generation until the central particle size L2 is 1 nm or more and 200 nm or less. It is characterized by being.

Due to these characteristics, by introducing bubbles B containing ozone (including microbubbles M) into the breeding water W, the solubility Z1 is 0.05 mg / l or more and 0.50 mg / l or less, It is possible to sterilize while reducing the damage to the aquatic animal S in the aquarium 2 as much as possible, it is possible to directly introduce the ozone microbubbles M into the breeding aquarium 2, and to filter the breeding water W It is no longer necessary to provide a separate sterilization facility, so that the sterilization facility can be made compact and the structure can be simplified, and the equipment cost can be reduced.
In addition, since ozone is supplied to the breeding water W as microbubbles M, ozone can stay in the breeding water W longer than the millimeter-order bubbles B, and the breeding water W can be convected. It is also possible for ozone to be present uniformly.

  Moreover, the ozone concentration Z1 in the breeding water W is set to 0.05 mg / l or more and 0.50 mg / l or less by setting the ozone concentration Z2 to 0.05 mg / l or more and 0.35 mg / l or less. Is possible.

  In addition to this, the bubbles B are generated by contracting nanobubbles N having a center particle size L2 of 1 nm to 500 nm and microbubbles M having a center particle size L1 of 1 μm to 50 μm to 1 nm to 200 nm. Nanobubbles N may be contained, and since the nanobubbles N can exist in the breeding water W for a longer period than the microbubbles M, the sterilization action by ozone lasts for a longer period, The running cost is reduced.

  According to the ozone disinfection apparatus according to the present invention, ozone microbubbles having a predetermined degree of dissolution are directly introduced into a breeding aquarium where aquatic animals are bred, so that there is no hindrance to the breeding of aquatic animals and ozone decontamination. Miniaturization of bacteria and sterilization facilities can be achieved.

It is a perspective view which shows the ozone disinfection apparatus which concerns on 1st Embodiment of this invention. It is a schematic diagram showing the ozone disinfection device of the first embodiment. It is a schematic diagram which shows the ozone disinfection apparatus of 2nd Embodiment.

Hereinafter, embodiments of the present invention will be described with reference to the drawings.
[First Embodiment]
FIG. 1 is a perspective view of an ozone sterilization apparatus 1 according to the first embodiment of the present invention.
The ozone sterilization apparatus 1 is used by being attached to a facility for breeding creatures at a farm or an aquarium. The ozone sterilization apparatus 1 is attached to a breeding aquarium 2 (such as for ginger and ornamental fish) that breeds aquatic animals S, and the breeding water W is seawater, brackish water, or fresh water.

  In addition, "sterilization" in the present invention means not only killing germs and parasites in the breeding water W by operation with the ozone sterilization apparatus 1, but also by touching ozone bubbles (ozone micro-nano bubbles) B, This includes the fact that the metabolism of the aquatic animal S proceeds and bacteria and the like are easily removed from the living body.

In the example shown in FIG. 1, fish (saltwater fish and freshwater fish) are bred as aquatic animals S in the breeding aquarium 2. The aquatic animal S according to the present invention includes not only seafood, whales and sea turtles, but also seaweeds such as kelp and sea turtles.
As shown in FIG. 2, the ozone sterilizer 1 includes ozone generating means 3 that generates ozone (ozone gas G) and bubbles (ozone micro-nano bubbles) B that contain ozone gas G generated by the ozone generating means 3. It has the production | generation means 4 and the direct entry means (bubble direct entry means) 5 which introduce | transduces the produced | generated ozone micro nano bubble B directly into the breeding water W of the breeding water tank 2. FIG.

In addition to this, the ozone sterilizer 1 includes a pump unit 11 that pumps the breeding water W from the breeding aquarium 2 and sends it to the bubble generating unit 4.
The ozone sterilizer 1 having the above-described means allows ozone to be dissolved in bubbles B containing microbubbles M and introduced into the breeding water W, so that the ozone solubility Z1 is 0.05 mg / l or more 0 .50 mg / l or less can be achieved.
Further, by entering directly in the form of microbubbles M, even ozone that is not so high in concentration can be dissolved in the liquid with high dissolution efficiency, and a sufficient sterilizing effect can be exhibited.

The ozone solubility Z1 is set to 0.05 mg / l or more and 0.50 mg / l or less when it is less than 0.05 mg / l, even if the ozone is easily dissolved by the microbubbles M This is because ozone cannot be sufficiently dissolved for sterilization in W and decomposition of residues.
This is because when the degree of dissolution Z1 is higher than 0.50 mg / l, not only sterilization in the breeding water W and decomposition of residues, but also the aquatic animal S is affected.

The ozone solubility Z1 is preferably 0.05 mg / l or more and 0.35 mg / l or less, and more preferably 0.10 mg / l or more and 0.25 mg / l or less.
In this way, by setting the inside of the breeding water W to an appropriate solubility Z1, it becomes possible to “directly introduce” the ozone bubbles B into the breeding aquarium 2 where the aquatic animal S exists, and filter the breeding water W. There is no need to provide a separate device.

The ozone solubility Z1 can be controlled by adjusting the ozone concentration Z2 contained in the microbubbles M. The ozone concentration Z2 is 0.05 mg / l or more and 0.35 mg / l or less, as will be described later. It is good to do.
The ozone concentration Z2 is preferably 0.05 mg / l or more and 0.25 mg / l or less, and more preferably 0.10 mg / l or more and 0.25 mg / l or less.

Here, the central particle size L of the bubbles B is important in order to maintain ozone in the breeding water W for a longer period while maintaining the appropriate solubility Z1.
The above-described bubbles B, that is, ozone micro-nano bubbles B include micro bubbles M having a center particle size L1 of 1 μm or more and 500 μm or less and nano bubbles N having a center particle size L2 of 1 nm or more and 500 nm or less.
The ozone micro-nano bubbles B may include micro and nano intermediate bubbles MN having a center particle size L3 of greater than 500 nm and less than 1 μm.

  These microbubbles M, nanobubbles N, and intermediate bubbles MN are generally spherical, and the center particle diameter L is the diameter of the bubbles B. The diameter L is the three-axis average diameter (see formula (1)) of the length a, the width b, and the thickness c of a circumscribed cuboid such as the microbubble M and nanobubble N (see formula (2)). ) The triaxial geometric mean diameter (see equations (3) and (4))

The surface of the microbubble M is negatively charged in the breeding water W, and the surface area per volume is larger than that of the bubble B having a center particle size L of millimeter order. It can be dissolved in the liquid.
In addition, the microbubbles M are characterized in that the pressure inside the bubble B is high, adhere to and separate from the surface of contaminants (residues in the breeding aquarium 2, miscellaneous bacteria, etc.), and remain in the liquid for a long time. There are things.

In particular, if the microbubbles M remain in the breeding water W for a long time, the rising speed of the bubbles B in the breeding water W varies greatly depending on the size. For example, the center particle size L is 3 mm. Millibubbles have an ascending speed of about 0.3 m / sec, whereas microbubbles M having a center particle size L of 100 μm have an ascent speed of about 0.005 m / sec.
When the center particle size L of the bubbles B is changed from the millimeter order to the micro order, the time during which ozone stays in the breeding water W is drastically increased. Moreover, since the microbubbles M are difficult to merge with each other, they are likely to be present in the breeding water W at a high density. This also makes it easy to increase the ozone solubility Z1.

  Since the number of bubbles B per unit volume of water varies depending on the size of the central particle size L, the solubility Z1 can be controlled. For example, when the center particle diameter L1 of the microbubbles M is 20 μm, approximately 600,000 bubbles B can exist in 1 ml of water.

As described above, the center particle diameter L1 of the microbubbles M is 1 μm or more and 500 μm or less, preferably 1 μm or more and 100 μm or less, and more preferably 1 μm or more and 50 μm or less.
In particular, when the microbubbles M having a central particle diameter L1 of 1 μm or more and 50 μm or less are introduced into the breeding water W, surface tension acts on the interface of the microbubbles M, and most of them start to contract after introduction.

More specifically, the surface tension is a force that attracts molecules to condense each other, and if it is a droplet, it tends to be spherical.
This is the same even if the microbubbles M in the breeding water W are about to become spherical due to the surface tension acting on the interface between the breeding water W and the ozone gas G.

Further, since the surface tension acting at the interface acts so as to attract toward the center of the sphere, the atmospheric pressure (internal pressure) of the ozone gas G inside the microbubble M is increased.
The action of increasing the internal pressure becomes stronger as the central particle diameter L1 of the microbubbles M becomes smaller, and the internal pressure of the microbubbles M increases in inverse proportion to the central particle diameter L1.

For example, in the microbubble M having a central particle diameter L1 of 10 μm, the internal pressure is increased by 0.3 atm from the normal pressure (1 atm), and in the microbubble M having the center particle diameter L1 of 1 μm, the internal pressure is 3.0 by more than the normal pressure. The atmospheric pressure also rises.
Note that the ozone gas G has a higher ability to dissolve in the breeding water W as its pressure increases.

In addition, as described above, as a feature, the surface of the microbubble M is negatively charged in the breeding water W. Due to this charging, the surface of the microbubble M is positively present in the breeding water W. Many ions gather (concentration of charge).
This concentration of charge generates free radicals on the surface of the microbubble M.

A free radical is a hydroxyl group (hydroxyl radical) that has one electron (unpaired electron) on the orbit of an atom, and the unpaired electron exchanges electrons with other molecules to become stable. Such hydroxyl radicals react strongly with surrounding molecules, and as a result, it is possible to decompose residues, germs, etc. in the breeding water W, which are contaminants.
Furthermore, since extremely high concentration charges are concentrated at the interface of the microbubbles M, an electrostatic repulsive force acting between the charges on the opposite sides of the bubble sphere works, and the bubbles B are formed from a predetermined center particle size L. Prevent shrinking.

As a result, the microbubble M starts to contract if the central particle size L1 at the time of generation is 1 μm or more and 50 μm or less, but when it contracts to 1 nm or more and 200 nm or less, the contraction stops due to electrostatic repulsion, Nanobubbles N are generated.
In the process of generating the nanobubbles N, if the center particle size L3 is stable at a value greater than 500 nm and less than 1 μm, an intermediate bubble MN is generated.

If ions concentrate on the interface of the microbubbles M, it becomes difficult to dissolve the ozone gas G inside the microbubbles M in the breeding water W in addition to the generation of free radicals. Stabilizes temporarily.
However, the bubbles B stabilized as the nanobubbles N or the intermediate bubbles MN are not stable forever, and usually the ions are out of balance and disappear in about several hours.

Therefore, by dissolving ions of a predetermined concentration in the breeding water W in advance (if the breeding water W is seawater, it is an electrolyte solution such as sodium, potassium, iron, etc., so ions are dissolved at a necessary concentration). By making the concentration of ions at the microbubble M interface sufficiently high, increasing the speed at which the microbubbles M introduced into the breeding water W are stimulated and reduced, and the ions are extremely concentrated around the bubbles B , Stabilized nanobubbles N and intermediate bubbles MN can be generated.
Moreover, in order to apply an impact to the microbubbles M in the breeding water W, ultrasonic generation means may be provided which starts contraction by applying ultrasonic vibration to the bubbles B in the bubble generation means 4.

  The generation method of the nano bubble N and the intermediate bubble MN is not limited to the case where ultrasonic vibration is applied, but in the state insulated from the breeding water W in the breeding aquarium 2, performing underwater discharge that starts contraction of the bubbles B, The microbubbles M generated by the gas-liquid shearing method may be forcibly contracted, or a stronger swiveling force may be applied to the microbubbles M, or ozone gas G or air may be injected into the liquid to which the surfactant is added. Thus, nanobubbles N may be generated.

As described above, the bubble B is composed of not only the microbubble M but also the nanobubble N and the intermediate bubble MN, so that the microbubble M helps to dissolve ozone, and at the same time, The sterilization effect lasts long due to nanobubbles N that sometimes exist for a long period of several months.
Furthermore, even when the aquatic animal S is activated by nanobubbles N or the like, germs and parasites are separated and sterilized.

Although the bubbles B such as the microbubbles M and nanobubbles N have been described above, the means 3 to 5 and 11 such as the ozone generating means 3 of the ozone sterilization apparatus 1 will be described.
As shown in FIG. 2, the ozone generating means 3 is a system that generates ozone gas G using an ultraviolet lamp method, an electrolysis method, a discharge method or the like. If it consists of an irradiation system and it is an electrolysis method, it becomes a system which applies a voltage between the both poles which sandwiched the electrolyte membrane in water.

If the system by the discharge method is explained in detail, the ozone generating means 3 becomes the discharge system 3 that performs silent discharge in oxygen. The discharge system 3 has a pair of electrodes in a flat plate shape spaced apart in oxygen, and these electrodes are covered with a high dielectric constant insulator (such as mica). Silent discharge is generated by applying an alternating voltage between the electrodes, and oxygen molecules between the electrodes are dissociated and recombined with other oxygen molecules to generate ozone gas G.
The discharge system 3 can adjust the concentration Z2 of the generated ozone gas G by changing the wavelength and intensity of ultraviolet rays emitted from a mercury lamp or the like, the voltage applied between the electrodes, and the oxygen concentration as a raw material.

As shown in FIG. 2, the bubble generating means 4 uses a gas-liquid shearing method as a method for generating the microbubbles M. Note that the microbubbles M may be generated not by the gas-liquid shearing method but by a pore (filter) method, a pressure dissolution method, a shock wave method, an ultrasonic method, or the like.
The bubble generating means 4 turns the ozone gas G into micro-nano bubbles by a gas-liquid shearing method in which the ozone gas G is entrained in a vortex rotating at 400 to 600 revolutions per second, and is cut and pulverized by an internal spiral pipe or a fan.

The bubble generating means 4 in the case of the gas-liquid shearing method is a bubble generator 12, and this bubble generator 12 is connected to the above-described pump means 11 for pumping the breeding water W from the breeding aquarium 2 via a hose 11a. The liquid and the ozone gas G are mixed in a tubular body having a spiral inner wall surface and stirred while swirling, whereby the bubbles in the liquid are refined to generate microbubbles M. The liquid mixed with the ozone gas G may be seawater or fresh water flowing from a supply pipe (not shown).
That is, the bubble generator 12 mixes and stirs the ozone gas G supplied from the ozone generation means 3 through the gas suction passage 12a with seawater or the like sent through the liquid supply passage 12b of the bubble generator 12. To generate microbubbles M containing ozone.

As shown in FIG. 2, the bubble direct entry means 5 directly injects the microbubbles M containing ozone into the breeding water W in the breeding aquarium 2 where the aquatic animals S are actually raised. Is an aeration case 13 for directly introducing the water into the breeding water W.
The air diffuser case 13 includes an air diffuser 13a connected to the tip of the bubble generator 12, a case box 13b for housing the air diffuser 13a, and a filter 13c provided on the upper surface of the case box 13b. ing.

In the air diffuser case 13, ozone microbubbles M are sent from the bubble generator 12 to the air diffuser 13a, and the ozone microbubbles M sent are passed through the holes 13d of the air diffuser 13a through the filter 13c and the breeding water W Is released (directly introduced).
The bubble direct entry means 5 does not necessarily have the diffuser case 13, and the microbubbles M may be introduced directly into the breeding water W from the tip of the bubble generator 12. At this time, the bubble generator 12 serves as both the bubble generation means 4 and the bubble direct entry means 5.

The microbubble M at the time of generation is a bubble B having a center particle diameter L1 of 1 μm or more and 500 μm or less, and is generated by changing the rotational speed of the vortex and the inner diameter of the tubular body of the bubble generator 12. The central particle diameter L1 of the bubble M can be adjusted.
By adjusting the central particle size L1 of the microbubbles M and the concentration Z2 of ozone (ozone gas G) generated by the ozone generating means 3, the solubility Z1 of ozone in the breeding water W is set appropriately. You can control the value.

  Table 1 shows that when the central particle diameter L1 of the microbubbles M is 1, 50, 100, 500, 1000 μm, the ozone concentration Z2 by the ozone generating means 3 is 0.001, 0.050, 0.100. , 0.200, 0.350, and 0.500 mg / l, the ozone solubility Z1 in the breeding water W is shown.

As shown in Table 1, when the ozone concentration Z2 by the ozone generating means 3 was 0.001 mg / l, the breeding water W Since the solubility Z1 of ozone in the inside is less than 0.05 mg / l, the breeding water W cannot be sufficiently sterilized.
On the contrary, when the ozone concentration Z2 is 0.500 mg / l, the ozone solubility Z1 in the breeding water W is 0.50 mg / l regardless of the central particle size L1 of the microbubbles M. As a result, the aquatic animal S in the breeding water W is affected.

  Therefore, by setting the concentration Z2 of ozone generated by the ozone generating means 3 to 0.05 mg / l or more and 0.35 mg / l or less, the ozone is contained in the microbubbles M which are micro-order bubbles B and reared. If introduced directly into the water W, the breeding water W can be effectively sterilized as long as there is no hindrance to the aquatic animal S, and there is no need to provide a separate filtration device for the breeding water W. Compactness and simplification are achieved, and equipment costs can be reduced.

EXAMPLES Hereinafter, although an Example demonstrates this invention in detail, this invention is limited to the following description and is not interpreted.
[Example 1]
Ozone microbubbles M (microbubbles B measured with a dynamic light scattering photometer with a central particle size L1 of 1 μm or more and 500 μm or less) are directly introduced into the breeding water W for sterilization test did.

In Example 1, when the solubility Z1 of ozone in the breeding water W is 0.01, 0.05, 0.15, 0.25, 0.35, 0.45, 0.55 mg / l In addition, the microbubbles M each containing ozone are directly entered into the breeding water W to kill coliform bacteria and fish general bacteria (pathogenic virus / IHNV, birnavirus, rhabdovirus, herpesvirus).
In the breeding aquarium 2 that breeds red sea bream as a live fish, ozone microbubbles M are directly introduced into the breeding water W, and the fish and shellfish are washed and sterilized together with the breeding water W so that they do not die. I investigated.

The ozone sterilization apparatus 1 shown in FIG. 1 uses a 200 liter (l) fish tank as a breeding aquarium 2, and breeds 60 red sea bream fish with an average body length of 5 cm in 170 liters of artificial seawater. The circulating flow rate when returning to the breeding water tank 2 again from the pump means 11 that sucked the breeding water W from the breeding water tank 2 through the bubble generating means 4 and the bubble direct entry means 5 was 3 liters / minute.
When operating under this condition, the ozone solubility Z1 of the breeding water W naturally increases, but the ozone generation means 3 adjusts the ozone concentration Z2 and the bubble generation means 4 adjusts the central particle size L1 of the microbubbles M1. Therefore, the degree of dissolution Z1 was kept constant at each numerical value.

Table 2 shows that the ozone solubility Z1 is maintained at 0.01, 0.05, 0.15, 0.25, 0.35, 0.45, and 0.55 mg / l before the direct entry of the microbubble M. 1 shows the number of coliforms (MPN / 100 ml), the number of general bacteria (CFU / ml), and the state of red sea bream (dead state) 1 hour after direct entry.
In addition, MPN in the unit of the number of coliforms is the most probable number by the abbreviation of Most Probable Number, and CFU in the unit of the number of general bacteria is the abbreviation of Colony Forming Unit and indicates the number of colony forming units.

As shown in Table 2, when the ozone solubility Z1 is 0.01 mg / l, there is no significant change in the number of coliform bacteria and general bacteria in the breeding water W; It increases when you do.
When the solubility Z1 was 0.55 mg / l, some red sea bream larvae, which were aquatic animals S, were weak, or some were damaged, such as dying fry or cannibalism being eaten away by cannibalism.

However, if the ozone solubility Z1 is 0.05 mg / l or more and 0.50 mg / l or less, it is possible to reduce the coliform bacteria and general bacteria in the breeding water W and to obtain a sufficient sterilization effect. There is no abnormality in swimming, and the influence of the aquatic animal S weakening and the breeding state are not hindered.
In addition, ozone can be produced inexpensively because the raw material is oxygen present in the air, and returns to oxygen after a sterilization reaction such as destroying cell membranes such as germs in the breeding water W. Therefore, there is no persistence.

Moreover, since the microbubble M containing ozone remains in the breeding water W for a while after direct entry (1 minute or more and 10 minutes or less), it is possible to reduce the frequency of sterilization by ozone, and the economic efficiency is improved.
Further, since no marine pharmaceuticals or salts are used for sterilization and the amount of ozone used is small, the burden on the environment is small and the safety of the human body consuming the aquatic animal S is high.

Because the ozone in the microbubbles M stays in the breeding water W for a long time, the ozone is easily decomposed and reduced to oxygen in the breeding water W, and as a result, it is difficult for the ozone to dissipate from the water surface. The ozone concentration in the environment is not high, and from this point on, the safety to the human body is high.
In addition, when the solubility Z1 is 0.05 mg / l or more and 0.50 mg / l or less, the growth rate of the aquatic animal S is increased, leading to growth promotion.

Specifically, the supply of ozone by the microbubbles M increases the oxygen consumption in the respiratory system of the aquatic organism S. At the same time, the blood flow is promoted to increase metabolism and increase the intake of food. Not only fungi but also growth promoting effects are seen.
Therefore, the aquatic animal S is not only promoted by being bred in a sterilized environment, but also with the nanobubbles N that exist for a long time, the respiratory system, digestive system, circulatory system, etc. Is activated, the metabolism is increased, and as a result, the amount of aquatic organisms S is increased.

[Second Embodiment]
FIG. 3 shows an ozone sterilization apparatus 1 according to the second embodiment.
A feature of the second embodiment is that the ozone sterilization apparatus 1 is provided with an aspirator 14 that increases the generation efficiency of nanobubbles N or intermediate bubbles MN.

The aspirator 14 includes a narrow tube portion 14a that is thinner than the inner diameter of the hose 11a of the pump means 11 and the liquid supply passage 12b of the bubble generator 12, and a gap portion 14b that communicates with the gas suction passage 12a downstream of the thin tube portion 14a. And have.
The thin tube portion 14a narrows the cross-sectional area of the flow of the fluid (bred water W) to increase the flow velocity, and the pressure in the gap portion 14b is lowered. Blow out into the portion 14b.

The mist-like breeding water W in the gap portion 14b draws in ozone gas G that has flowed into the gap portion 14b due to the pressure drop, and microbubble-like ozone gas G is generated in the breeding water W.
In addition, you may provide the non-return valve which can close the communicating port of the gas suction path 12a and the space | gap part 14b.
Even if this check valve should reduce the flow rate of the breeding water W in the liquid supply path 12b and the breeding water W leaks into the gap 14b without drawing the ozone gas G, the leaked pressure Thus, the communication port between the gas suction path 12a and the gap portion 14b is closed, the inflow of the breeding water W to the gas suction path 12a is prevented, and the ozone generation means 3 is prevented from being broken.

The check valve may have any shape as long as the communication port between the gas suction passage 12a and the gap portion 14b can be closed. For example, a spherical body having a diameter larger than that of the communication port is preferable.
Other configurations, operational effects, and specifications of the ozone sterilization apparatus 1 are the same as those in the first embodiment.

[Example 2]
In the sterilization test of Example 1, the microbubbles M generated by the aspirator 14 were directly input for 1 hour so that the ozone solubility Z1 was maintained at 0.25 mg / l, and ozone nanobubbles N and intermediate bubbles MN The same sterilization test as in Example 1 was performed except that the production efficiency was increased.
Table 3 shows 1 hour, 12 hours, 24 hours, 3 days, 1 week, 2 weeks, 4 weeks after the stop of direct entry of microbubbles M (bubble direct entry means 5) using the aspirator 14. The number of coliforms (MPN / 100 ml) and the number of general bacteria (CFU / ml) after 6 weeks are shown as Example 2-1.

  For comparison, the example 2-2 in Table 3 is one hour after the direct entry of the microbubbles M is stopped when the aspirator 14 is not attached, that is, when the generation efficiency of the nanobubbles N is not increased. , 12 hours, 24 hours, 3 days, 1 week, 2 weeks, 4 weeks, and 6 weeks, the number of coliforms and the number of general bacteria are shown.

As shown in Example 2-2 in Table 3, even when the aspirator 14 was not used (that is, the generation efficiency of nanobubbles N and intermediate bubbles MN was not increased), as described above, Since the microbubbles M naturally contract due to the increase of the self-internal pressure, nanobubbles N and the like are generated, and some of these nanobubbles N are present in the breeding water W for several hours, so the direct entry of the microbubbles M is stopped. At 1 hour, the number of coliform bacteria and general bacteria is not greatly increased.
However, after 12 hours have passed since the stop of direct entry of microbubbles M, the number of coliform bacteria and general bacteria has increased significantly with the passage of time. Is over.

On the other hand, Example 2-1 in which the generation of nanobubbles N and intermediate bubbles MN was performed with high efficiency was the stop of direct entry of the microbubbles M by the aspirator 14, and the number of coliform bacteria and general bacteria was already higher than in Example 2-2. The number is reduced, and the sterilization effect is improved even in a short time by improving the production rate of nanobubbles N and the like.
Furthermore, the number of coliforms and general bacteria does not increase greatly even after 4 weeks have passed since the direct entry of microbubbles M, and the number of coliforms exceeds the value before direct entry of microbubbles M for the first time. Six weeks after the stop of direct entry of microbubbles M, the sterilization effect has been maintained for a very long time due to the high efficiency of nanobubbles N and the like.

Therefore, by introducing the microbubbles M directly into the breeding water W, the nanobubbles N and the intermediate bubbles MN that are naturally generated in the breeding aquarium 2 can be effectively used without leaking, so that the sterilization effect lasts for about one hour, The intermittent operation of the ozone sterilization apparatus 1 that performs direct entry of the microbubbles M every predetermined time is possible, and the running cost of ozone sterilization is reduced.
As shown in Table 3 above, even in the case of Example 2-2 in which the aspirator 14 is not used, if the ozone sterilizer 1 is operated for 1 hour, the coliform group may be stopped even if the ozone sterilizer 1 is stopped for 1 hour thereafter. The number of general bacteria does not exceed the value before direct entry of the microbubble M.

Furthermore, as in Example 2-1, by attaching the aspirator 14 to increase the generation efficiency of the nanobubbles N and the intermediate bubbles MN, the existence period in the breeding water W is dramatically increased compared to the microbubbles M. As a result, the interval of intermittent operation of the ozone sterilizer 1 can be further extended.
For example, if the ozone sterilizer 1 is operated about once per month for about 1 hour and the microbubble M directly attached with the aspirator 14 (highly efficient generation of nanobubbles N, etc.) is performed, The number of general bacteria can be suppressed to a predetermined value or less.

In addition, this invention is not limited to embodiment mentioned above. Each configuration of the ozone sterilizer 1 or the like, or the overall structure, shape, dimensions, and the like can be appropriately changed in accordance with the spirit of the present invention.
The bubble generating means 4 is not limited to microbubbles that use only the water pressure from the pump means 11 as a power source, like the bubble generator 12 described above, and a large amount of ozone gas G in seawater, fresh water, etc. under high pressure. You may use the well-known microbubble generator provided with the power source, such as the pressurization decompression method which makes it re-bubble by decompressing after making it melt | dissolve.

In addition, as described above, in order to generate the nanobubbles N or the intermediate bubbles MN, there may be provided discharge means for impacting the microbubbles M in the breeding water W.
This discharge means has an anode and a cathode standing in a region insulated from the breeding water W in the breeding tank 2 of the aquatic animal S. A voltage of 2000 to 3000 V is applied between the two poles to Generate a discharge.

At this time, the breeding water W may be seawater as described above, but even if it is fresh water or brackish water, the conductivity of the breeding water W is 3 mS / cm by adding an electrolyte of iron, manganese, calcium or other minerals. An electrolyte may be added as described above.
In order to generate nanobubbles N and the like more efficiently, it is preferable that the ozone solubility Z1 in the breeding water W reaches 50% or more of the saturation solubility.

Due to the stimulation (physical stimulation) of the shock wave accompanying the underwater discharge of the discharge means, the ozone microbubbles M are rapidly reduced to become nanobubbles N or intermediate bubbles MN.
At this time, ions existing at the interface such as the nanobubbles N have a rapid reduction speed, so that they do not have time to deviate into the surrounding water and are rapidly concentrated as the bubbles are reduced. Concentrated ions exist stably in the breeding water W because the internal ozone such as the nanobubbles N prevents natural dissolution in the breeding water W.

  INDUSTRIAL APPLICABILITY The present invention can be used not only for a device for sterilizing breeding water in a miniaturized facility, but also for aquaculture devices that achieve increased yield, increased efficiency, reduced environmental and human effects, etc. by promoting growth of aquatic animals. .

DESCRIPTION OF SYMBOLS 1 Ozone sterilizer 2 Breeding tank 3 Ozone generating means 4 Bubble generating means 5 Bubble direct entry means (direct entry means)
S Aquatic animals W Breeding water B Bubbles (ozone micro-nano bubbles)
M Microbubble N Nanobubble L1 Microbubble center particle size L2 Nanobubble center particle size Z1 Ozone solubility Z2 Ozone concentration

Claims (4)

An ozone disinfection device for introducing bubbles (B) containing ozone into the breeding water (W) of the breeding tank (2) of the aquatic animal (S),
Ozone generating means (3) for generating ozone, bubble generating means (4) for generating bubbles (B) containing ozone generated by the ozone generating means (3), and bubble generating means (4) A direct entry means (5) for directly introducing the air bubbles (B) generated in (1) into the breeding water (W) of the breeding aquarium (2) where the aquatic animals (S) are raised,
The bubbles (B) include microbubbles (M) having a center particle size (L1) of 1 μm or more and 500 μm or less at the time of generation,
An ozone disinfection apparatus, wherein the degree of ozone dissolution (Z1) in the breeding water (W) is 0.05 mg / l or more and 0.50 mg / l or less.   The ozone disinfection apparatus according to claim 1, wherein the concentration (Z2) of ozone generated by the ozone generation means (3) is 0.05 mg / l or more and 0.35 mg / l or less.   The ozone sterilizer according to claim 1 or 2, wherein the bubbles (B) also contain nanobubbles (N) having a central particle size (L2) of 1 nm to 500 nm at the time of generation.   At least a part of the nanobubbles (N) contracts until the microbubble (M) having a center particle size (L1) of 1 μm or more and 50 μm or less at the time of generation is reduced to a center particle size (L2) of 1 nm or more and 200 nm or less. The ozone disinfection apparatus according to claim 3, wherein the ozone disinfection apparatus is produced.

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