CN107709545A - 从干细胞生成肌肉谱系细胞 - Google Patents

从干细胞生成肌肉谱系细胞 Download PDF

Info

Publication number
CN107709545A
CN107709545A CN201680035069.5A CN201680035069A CN107709545A CN 107709545 A CN107709545 A CN 107709545A CN 201680035069 A CN201680035069 A CN 201680035069A CN 107709545 A CN107709545 A CN 107709545A
Authority
CN
China
Prior art keywords
cells
pluripotent stem
satellite
pax3
pax7
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
CN201680035069.5A
Other languages
English (en)
Chinese (zh)
Inventor
乌利·施密特
莱斯利·卡伦
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Sonic Master Ltd
Original Assignee
Gina Intellectual Property Holdings Pte Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Gina Intellectual Property Holdings Pte Ltd filed Critical Gina Intellectual Property Holdings Pte Ltd
Publication of CN107709545A publication Critical patent/CN107709545A/zh
Pending legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0652—Cells of skeletal and connective tissues; Mesenchyme
    • C12N5/0658—Skeletal muscle cells, e.g. myocytes, myotubes, myoblasts
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
    • A61K35/12—Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells
    • A61K35/34—Muscles; Smooth muscle cells; Heart; Cardiac stem cells; Myoblasts; Myocytes; Cardiomyocytes
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P21/00—Drugs for disorders of the muscular or neuromuscular system
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P21/00—Drugs for disorders of the muscular or neuromuscular system
    • A61P21/04—Drugs for disorders of the muscular or neuromuscular system for myasthenia gravis
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0652—Cells of skeletal and connective tissues; Mesenchyme
    • C12N5/0658—Skeletal muscle cells, e.g. myocytes, myotubes, myoblasts
    • C12N5/0659—Satellite cells
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
    • G01N33/5008—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics
    • G01N33/5044—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics involving specific cell types
    • G01N33/5061—Muscle cells
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
    • A61K35/12—Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells
    • A61K35/48—Reproductive organs
    • A61K35/54—Ovaries; Ova; Ovules; Embryos; Foetal cells; Germ cells
    • A61K35/545—Embryonic stem cells; Pluripotent stem cells; Induced pluripotent stem cells; Uncharacterised stem cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/15—Transforming growth factor beta (TGF-β)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/40—Regulators of development
    • C12N2501/415—Wnt; Frizzeled
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2510/00—Genetically modified cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2533/00—Supports or coatings for cell culture, characterised by material
    • C12N2533/90—Substrates of biological origin, e.g. extracellular matrix, decellularised tissue

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Chemical & Material Sciences (AREA)
  • Cell Biology (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Organic Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • Rheumatology (AREA)
  • Immunology (AREA)
  • Medicinal Chemistry (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Developmental Biology & Embryology (AREA)
  • Veterinary Medicine (AREA)
  • Animal Behavior & Ethology (AREA)
  • Pharmacology & Pharmacy (AREA)
  • General Engineering & Computer Science (AREA)
  • Public Health (AREA)
  • Molecular Biology (AREA)
  • Hematology (AREA)
  • Urology & Nephrology (AREA)
  • Virology (AREA)
  • Epidemiology (AREA)
  • Cardiology (AREA)
  • Vascular Medicine (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Physical Education & Sports Medicine (AREA)
  • Orthopedic Medicine & Surgery (AREA)
  • Neurology (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Pathology (AREA)
  • General Physics & Mathematics (AREA)
CN201680035069.5A 2015-04-22 2016-04-22 从干细胞生成肌肉谱系细胞 Pending CN107709545A (zh)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201562151352P 2015-04-22 2015-04-22
US62/151,352 2015-04-22
PCT/AU2016/000144 WO2016168890A1 (en) 2015-04-22 2016-04-22 Generation of muscle-lineage cells from stem cells

Publications (1)

Publication Number Publication Date
CN107709545A true CN107709545A (zh) 2018-02-16

Family

ID=60254709

Family Applications (1)

Application Number Title Priority Date Filing Date
CN201680035069.5A Pending CN107709545A (zh) 2015-04-22 2016-04-22 从干细胞生成肌肉谱系细胞

Country Status (7)

Country Link
US (1) US20180346879A1 (https=)
EP (1) EP3286301B1 (https=)
JP (1) JP7055638B2 (https=)
CN (1) CN107709545A (https=)
AU (1) AU2016250905B2 (https=)
CA (1) CA2983332A1 (https=)
WO (1) WO2016168890A1 (https=)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN110859854A (zh) * 2018-08-08 2020-03-06 上海市东方医院 治疗神经源性肌肉萎缩的药物组合物及其制法和应用

Families Citing this family (15)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ES2731437T3 (es) 2014-11-21 2019-11-15 Regeneron Pharma Métodos y composiciones para la modificación genética dirigida mediante el uso de pares de ARN guías
WO2017100498A1 (en) 2015-12-11 2017-06-15 The Johns Hopkins University Isolation of fusion-competent myoblasts and therapeutic applications thereof related to muscular dystrophy
US20190338251A1 (en) * 2016-04-27 2019-11-07 Takeda Pharmaceutical Company Limited Skeletal Muscle Precursor Cells and Production Method for Skeletal Muscle Cells
IL323024A (en) 2016-05-20 2025-10-01 Regeneron Pharma Methods for breaking immunological tolerance using multiple guide RNAs
CN110730819A (zh) 2016-10-26 2020-01-24 索尼克马斯特有限公司 肌肉谱系细胞的改善产生及其治疗用途
WO2018128779A1 (en) * 2017-01-06 2018-07-12 The Regents Of The University Of California Methods for generating skeletal muscle progenitor cells
CN107937338B (zh) * 2017-11-30 2021-08-03 中山大学 一种来自多能干细胞的中胚层谱系间充质干细胞及其制备方法
JP7033095B2 (ja) * 2019-03-04 2022-03-09 日清食品ホールディングス株式会社 三次元筋組織とその製造方法
WO2021102324A1 (en) * 2019-11-20 2021-05-27 Vita Therapeutics, Inc. Method of engineering hypoimmunogenic muscle precursor cells
JP7540893B2 (ja) * 2020-02-27 2024-08-27 テルモ株式会社 Ｃｄ５６陽性細胞の比率を高めるための組成物
CN111826451B (zh) * 2020-09-11 2026-02-03 中国肉类食品综合研究中心 实时荧光定量pcr检测猪肌肉调节因子的引物组及其应用
WO2022093665A1 (en) * 2020-10-26 2022-05-05 University Of Massachusetts Methods and compositions for treatment of muscle disease with ipsc-induced human skeletal muscle stem cells
WO2022140300A1 (en) * 2020-12-22 2022-06-30 Ixcells Biotechnolgies Usa, Inc. Methods and compositions for differentiating skeletal muscle
US20240360415A1 (en) * 2021-08-09 2024-10-31 Aleph Farms Ltd. Muscle cells differentiated from pluripotent cells, methods of producing same and use thereof
KR20240152443A (ko) * 2023-04-12 2024-10-22 이엔셀 주식회사 근력 약화 관련 질환 치료제 스크리닝 방법

Citations (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101426902A (zh) * 2006-04-28 2009-05-06 阿斯比奥制药株式会社 将多能性干细胞分化诱导成心肌细胞的方法
CN103228782A (zh) * 2010-06-13 2013-07-31 中国科学院生物物理研究所 由干细胞制备心肌细胞的方法和组合物及其用途
WO2013138623A1 (en) * 2012-03-14 2013-09-19 Children's Medical Center Corporation High-throughput image-based chemical screening in zebrafish blastomere cell culture
CN104293730A (zh) * 2014-09-23 2015-01-21 云南中科灵长类生物医学重点实验室 多能干细胞体外定向分化为心肌细胞的方法
CN104508121A (zh) * 2012-07-23 2015-04-08 中国科学院生物物理研究所 体外诱导多能干细胞分化为心室肌细胞的方法
AU2014268197A1 (en) * 2014-02-11 2015-08-27 Monash University Muscle cell production
WO2016108288A1 (ja) * 2014-12-29 2016-07-07 国立大学法人京都大学 骨格筋前駆細胞の製造方法

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5143842A (en) 1988-11-01 1992-09-01 The University Of Colorado Foundation, Inc. Media for normal human muscle satellite cells

Patent Citations (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101426902A (zh) * 2006-04-28 2009-05-06 阿斯比奥制药株式会社 将多能性干细胞分化诱导成心肌细胞的方法
CN103228782A (zh) * 2010-06-13 2013-07-31 中国科学院生物物理研究所 由干细胞制备心肌细胞的方法和组合物及其用途
WO2013138623A1 (en) * 2012-03-14 2013-09-19 Children's Medical Center Corporation High-throughput image-based chemical screening in zebrafish blastomere cell culture
CN104508121A (zh) * 2012-07-23 2015-04-08 中国科学院生物物理研究所 体外诱导多能干细胞分化为心室肌细胞的方法
AU2014268197A1 (en) * 2014-02-11 2015-08-27 Monash University Muscle cell production
CN104293730A (zh) * 2014-09-23 2015-01-21 云南中科灵长类生物医学重点实验室 多能干细胞体外定向分化为心肌细胞的方法
WO2016108288A1 (ja) * 2014-12-29 2016-07-07 国立大学法人京都大学 骨格筋前駆細胞の製造方法

Non-Patent Citations (4)

* Cited by examiner, † Cited by third party
Title
ALESSANDRA CASTIGLIONI等: "Isolation of Progenitors that Exhibit Myogenic/Osteogenic Bipotency In Vitro", 《STEM CELL REPORTS》 *
AMER MAHMOOD等: "Enhanced Differentiation of Human Embryonic Stem Cells to Mesenchymal Progenitors by Inhibition of TGF-beta/Activin/Nodal Signaling Using SB-431542", 《JOURNAL OF BONE AND MINERAL RESEARCH》 *
BIANCA BORCHIN等: "Derivation and FACS-Mediated Purification of PAX3+/PAX7+ Skeletal Muscle Precursors from Human Pluripotent Stem Cells", 《STEM CELL REPORTS》 *
MICHAEL SHELTON等: "Derivation and Expansion of PAX7-Positive Muscle Progenitors from Human and Mouse Embryonic Stem Cells", 《STEM CELL REPORTS》 *

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN110859854A (zh) * 2018-08-08 2020-03-06 上海市东方医院 治疗神经源性肌肉萎缩的药物组合物及其制法和应用

Also Published As

Publication number Publication date
AU2016250905A8 (en) 2018-03-15
WO2016168890A8 (en) 2017-11-30
AU2016250905A1 (en) 2017-11-09
JP2018518943A (ja) 2018-07-19
CA2983332A1 (en) 2016-10-27
EP3286301A4 (en) 2019-02-20
AU2016250905B2 (en) 2022-02-10
US20180346879A1 (en) 2018-12-06
EP3286301A1 (en) 2018-02-28
EP3286301B1 (en) 2021-07-28
JP7055638B2 (ja) 2022-04-18
WO2016168890A1 (en) 2016-10-27

Similar Documents

Publication Publication Date Title
CN107709545A (zh) 从干细胞生成肌肉谱系细胞
US20250197802A1 (en) Method for reproducible differentiation of clinical-grade retinal pigment epithelium cells
US20250283051A1 (en) Cells differentiated from immunoengineered pluripotent cells
Goudenege et al. Myoblasts derived from normal hESCs and dystrophic hiPSCs efficiently fuse with existing muscle fibers following transplantation
Piccoli et al. Amniotic fluid stem cells restore the muscle cell niche in a HSA‐Cre, SmnF7/F7 mouse model
JP2023162277A (ja) Ｍａｃｓを用いた幹細胞由来網膜色素上皮の精製
JP6482005B2 (ja) 網膜前駆体、網膜色素上皮細胞及び神経網膜細胞を得るための方法
JP6473686B2 (ja) 細胞の選別方法
US20240002811A1 (en) Methods for Generation for Pluripotent and Multipotent Cells
EP1248834A1 (en) Human embryoid body-derived cells
CN117982733A (zh) 可生物降解的组织置换植入物及其用途
JP2026032026A (ja) 網膜色素上皮細胞を生成するための方法
Bonilla-Pons et al. Müller glia fused with adult stem cells undergo neural differentiation in human retinal models
US20230045590A1 (en) Genetically corrected cells for therapeutic use
US12529033B2 (en) Method of isolating myogenic progenitors using CD54
US20250197803A1 (en) A method of differentiating an induced pluripotent stem cell into a retinal pigment epithelial cell, a retinal pigment epithelial cell and methods of using the retinal pigment epithelial cell
Bonsi et al. Stem cells
HK40077488A (en) Method for reproducible differentiation of clinical-grade retinal pigment epithelium cells
Ghiassi Engineering A Retinal Progenitor Cell Graft For Transplantation Studies In The Rd10 Mouse Model
CN118974253A (zh) Crispr v型系统的治疗应用
Alvarez Stem Cell Biology and Strategies for Therapeutic Development in Degenerative Diseases and Cancer
Kim Directed Differentiation of Human Embryonic Stem Cells into Dopamine Neurons
Chichagova Investigating retinal pathology in patients carrying m. 3243A> G mutation using human induced pluripotent stem cells
Hewitt Epigenetic Regulation of Pluripotent Stem Cell Differentiation

Legal Events

Date Code Title Description
PB01 Publication
PB01 Publication
SE01 Entry into force of request for substantive examination
SE01 Entry into force of request for substantive examination
TA01 Transfer of patent application right

Effective date of registration: 20180929

Address after: American California

Applicant after: Gina biological cell American holding company

Address before: New South Wales Australia

Applicant before: Gina Intellectual Property Holdings Pte Ltd

TA01 Transfer of patent application right
TA01 Transfer of patent application right

Effective date of registration: 20190829

Address after: The British Virgin Islands of Tortola

Applicant after: Sony Kmaster Co., Ltd.

Address before: American California

Applicant before: Gina biological cell American holding company

TA01 Transfer of patent application right