CA2983332A1 - Generation of muscle-lineage cells from stem cells - Google Patents

Generation of muscle-lineage cells from stem cells

Info

Publication number
CA2983332A1
CA2983332A1 CA2983332A CA2983332A CA2983332A1 CA 2983332 A1 CA2983332 A1 CA 2983332A1 CA 2983332 A CA2983332 A CA 2983332A CA 2983332 A CA2983332 A CA 2983332A CA 2983332 A1 CA2983332 A1 CA 2983332A1
Authority
CA
Canada
Prior art keywords
cells
pluripotent stem
cell
satellite
pax3
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
CA2983332A
Other languages
English (en)
French (fr)
Inventor
Uli Schmidt
Leslie Caron
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Sonic Master Ltd
Original Assignee
Genea IP Holdings Pty Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Genea IP Holdings Pty Ltd filed Critical Genea IP Holdings Pty Ltd
Publication of CA2983332A1 publication Critical patent/CA2983332A1/en
Pending legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0652—Cells of skeletal and connective tissues; Mesenchyme
    • C12N5/0658—Skeletal muscle cells, e.g. myocytes, myotubes, myoblasts
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
    • A61K35/12—Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells
    • A61K35/34—Muscles; Smooth muscle cells; Heart; Cardiac stem cells; Myoblasts; Myocytes; Cardiomyocytes
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P21/00—Drugs for disorders of the muscular or neuromuscular system
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P21/00—Drugs for disorders of the muscular or neuromuscular system
    • A61P21/04—Drugs for disorders of the muscular or neuromuscular system for myasthenia gravis
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0652—Cells of skeletal and connective tissues; Mesenchyme
    • C12N5/0658—Skeletal muscle cells, e.g. myocytes, myotubes, myoblasts
    • C12N5/0659—Satellite cells
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
    • G01N33/5008—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics
    • G01N33/5044—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics involving specific cell types
    • G01N33/5061—Muscle cells
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
    • A61K35/12—Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells
    • A61K35/48—Reproductive organs
    • A61K35/54—Ovaries; Ova; Ovules; Embryos; Foetal cells; Germ cells
    • A61K35/545—Embryonic stem cells; Pluripotent stem cells; Induced pluripotent stem cells; Uncharacterised stem cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/15—Transforming growth factor beta (TGF-β)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/40—Regulators of development
    • C12N2501/415—Wnt; Frizzeled
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2510/00—Genetically modified cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2533/00—Supports or coatings for cell culture, characterised by material
    • C12N2533/90—Substrates of biological origin, e.g. extracellular matrix, decellularised tissue

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Chemical & Material Sciences (AREA)
  • Cell Biology (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Organic Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Rheumatology (AREA)
  • Genetics & Genomics (AREA)
  • Immunology (AREA)
  • Medicinal Chemistry (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Developmental Biology & Embryology (AREA)
  • General Engineering & Computer Science (AREA)
  • Veterinary Medicine (AREA)
  • Public Health (AREA)
  • Animal Behavior & Ethology (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Molecular Biology (AREA)
  • Urology & Nephrology (AREA)
  • Hematology (AREA)
  • Epidemiology (AREA)
  • Vascular Medicine (AREA)
  • Cardiology (AREA)
  • Virology (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Neurology (AREA)
  • Orthopedic Medicine & Surgery (AREA)
  • Physical Education & Sports Medicine (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Toxicology (AREA)
  • Food Science & Technology (AREA)
CA2983332A 2015-04-22 2016-04-22 Generation of muscle-lineage cells from stem cells Pending CA2983332A1 (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201562151352P 2015-04-22 2015-04-22
US62/151,352 2015-04-22
PCT/AU2016/000144 WO2016168890A1 (en) 2015-04-22 2016-04-22 Generation of muscle-lineage cells from stem cells

Publications (1)

Publication Number Publication Date
CA2983332A1 true CA2983332A1 (en) 2016-10-27

Family

ID=60254709

Family Applications (1)

Application Number Title Priority Date Filing Date
CA2983332A Pending CA2983332A1 (en) 2015-04-22 2016-04-22 Generation of muscle-lineage cells from stem cells

Country Status (7)

Country Link
US (1) US20180346879A1 (https=)
EP (1) EP3286301B1 (https=)
JP (1) JP7055638B2 (https=)
CN (1) CN107709545A (https=)
AU (1) AU2016250905B2 (https=)
CA (1) CA2983332A1 (https=)
WO (1) WO2016168890A1 (https=)

Families Citing this family (16)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR102531016B1 (ko) 2014-11-21 2023-05-10 리제너론 파마슈티칼스 인코포레이티드 쌍 형성된 가이드 rna를 사용하는 표적화된 유전자 변형을 위한 방법 및 조성물
WO2017100498A1 (en) 2015-12-11 2017-06-15 The Johns Hopkins University Isolation of fusion-competent myoblasts and therapeutic applications thereof related to muscular dystrophy
WO2017188458A1 (ja) * 2016-04-27 2017-11-02 武田薬品工業株式会社 骨格筋前駆細胞及び骨格筋細胞の製造方法
CN113831407B (zh) 2016-05-20 2024-06-11 瑞泽恩制药公司 用于使用多个引导rna来破坏免疫耐受性的方法
AU2017351638A1 (en) * 2016-10-26 2019-06-13 Genea Biocells USA (Holdings), Inc. Improved generation of muscle lineage cells and therapeutic uses thereof
EP3565887B1 (en) * 2017-01-06 2025-07-16 The Regents of The University of California Methods for generating skeletal muscle progenitor cells
CN107937338B (zh) * 2017-11-30 2021-08-03 中山大学 一种来自多能干细胞的中胚层谱系间充质干细胞及其制备方法
CN110859854A (zh) * 2018-08-08 2020-03-06 上海市东方医院 治疗神经源性肌肉萎缩的药物组合物及其制法和应用
JP7033095B2 (ja) * 2019-03-04 2022-03-09 日清食品ホールディングス株式会社 三次元筋組織とその製造方法
WO2021102324A1 (en) * 2019-11-20 2021-05-27 Vita Therapeutics, Inc. Method of engineering hypoimmunogenic muscle precursor cells
JP7540893B2 (ja) * 2020-02-27 2024-08-27 テルモ株式会社 Ｃｄ５６陽性細胞の比率を高めるための組成物
CN111826451B (zh) * 2020-09-11 2026-02-03 中国肉类食品综合研究中心 实时荧光定量pcr检测猪肌肉调节因子的引物组及其应用
US20240018479A1 (en) * 2020-10-26 2024-01-18 University Of Massachusetts Methods and compositions for treatment of muscle disease with ipsc-induced human skeletal muscle stem cells
WO2022140300A1 (en) * 2020-12-22 2022-06-30 Ixcells Biotechnolgies Usa, Inc. Methods and compositions for differentiating skeletal muscle
CN117795059A (zh) * 2021-08-09 2024-03-29 阿利夫农场公司 从多能细胞分化的肌细胞、其产生方法和用途
KR20240152443A (ko) * 2023-04-12 2024-10-22 이엔셀 주식회사 근력 약화 관련 질환 치료제 스크리닝 방법

Family Cites Families (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5143842A (en) 1988-11-01 1992-09-01 The University Of Colorado Foundation, Inc. Media for normal human muscle satellite cells
KR101346047B1 (ko) * 2006-04-28 2013-12-31 다이이찌 산쿄 가부시키가이샤 다능성 줄기 세포로부터 심근 세포의 분화를 유도하는 방법
WO2011157029A1 (en) * 2010-06-13 2011-12-22 Institute Of Biophysics, Chinese Academy Of Sciences Methods and compositions for preparing cardiomyocytes from stem cells and uses thereof
EP2825637A4 (en) * 2012-03-14 2015-12-23 Childrens Medical Center PICTORIAL-BASED CHEMICAL SCREENING WITH HIGH PASSAGE IN A ZEBRAFISH BLASTOMER CELL CULTURE
CA2886396C (en) * 2012-07-23 2023-08-22 Institute Of Biophysics, Chinese Academy Of Sciences Method for inducing pluripotent stem cells to differentiate into ventricular cardiomyocytes in vitro
AU2014268197A1 (en) * 2014-02-11 2015-08-27 Monash University Muscle cell production
CN104293730B (zh) * 2014-09-23 2017-07-28 云南中科灵长类生物医学重点实验室 多能干细胞体外定向分化为心肌细胞的方法
JP6845500B2 (ja) * 2014-12-29 2021-03-17 国立大学法人京都大学 骨格筋前駆細胞の製造方法

Also Published As

Publication number Publication date
AU2016250905A8 (en) 2018-03-15
CN107709545A (zh) 2018-02-16
JP7055638B2 (ja) 2022-04-18
WO2016168890A8 (en) 2017-11-30
WO2016168890A1 (en) 2016-10-27
EP3286301A1 (en) 2018-02-28
AU2016250905A1 (en) 2017-11-09
JP2018518943A (ja) 2018-07-19
EP3286301B1 (en) 2021-07-28
EP3286301A4 (en) 2019-02-20
US20180346879A1 (en) 2018-12-06
AU2016250905B2 (en) 2022-02-10

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