CN107698300A - A kind of compound fixed nitrogen microbial manure and preparation method thereof - Google Patents
A kind of compound fixed nitrogen microbial manure and preparation method thereof Download PDFInfo
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- CN107698300A CN107698300A CN201610644361.9A CN201610644361A CN107698300A CN 107698300 A CN107698300 A CN 107698300A CN 201610644361 A CN201610644361 A CN 201610644361A CN 107698300 A CN107698300 A CN 107698300A
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- series bacillus
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05B—PHOSPHATIC FERTILISERS
- C05B7/00—Fertilisers based essentially on alkali or ammonium orthophosphates
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- Pest Control & Pesticides (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
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Abstract
The present invention discloses a kind of compound fixed nitrogen microbial manure and preparation method thereof, and compound fixed nitrogen microbial manure includes following raw material：15~20 kilograms of 20~30 kilograms of fixed nitrogen series bacillus bacterium solution, 80~double centner of humic acid, 800~900 kilograms of powdered rice hulls, 15~20 kilograms of urea, 20~30 kilograms of Diammonium phosphate (DAP) and potassium sulfate.During preparation, the bacterium solution after fixed nitrogen series bacillus is fermented mixes with humic acid, powdered rice hulls, urea, Diammonium phosphate (DAP) and potassium sulfate.The compound fixed nitrogen microbial manure being prepared by using inventive formulation and method is powdery and bottom application type fertilizer, fertilization time is applied initial stage in planting, purpose is to provide nutrition needed for plant growth, promotes plant growth, improves chemical fertilizer utilization rate, reduce pollution, pest and disease damage is reduced, strengthens the anti-adversity ability of crops, improves crops fruit quality and yield, play an important roll, can be promoted the use of in agricultural production.
Description
Technical field
The present invention relates to microbial manure art, refers in particular to a kind of compound fixed nitrogen microbial manure and its preparation side
Method.
Background technology
Excessively apply and rely on chemical nitrogen manure brings huge negative effect to agricultural production and ecological environment for a long time.It is long
Phase excessively applies chemical fertilizer particularly chemical nitrogen fertilizer partially, and the accumulation of one side nitrate has and causes soil acidification and salination, soil
The trend of earth microbial activity decline etc.；Simultaneously excessive nitrogen cause it is counter digest, cause nitrous acid in the agricultural product such as veterinary antibiotics
Salt is exceeded, seriously endangers human health, on the other hand adds the contaminated risk of water body, and oneself turns into agricultural sustainable development
One important restrictive factor.
Nitrogen in air can be reduced into ammonium by nitrogen-fixing microorganism at normal temperatures and pressures, using internal azotase, for planting
Thing growth utilizes.Nitrogen-fixing microorganism species is different, and their characteristic is also variant.Fixed nitrogen series bacillus, which removes, has Characteristics of Nitrogen Fixation
Outside, also there is very strong resistance against disadvantage, i.e., the strong gemma of resistance against disadvantage, condition are formed under the adverse environmental factors such as high temperature, low temperature, drying
Restoration ecosystem when suitable, the microorganism of gene gemma is the optimal strain for preparing microbial manure.
The content of the invention
In view of this, in view of the existing deficiencies of the prior art, it is micro- that its main purpose is to provide a kind of compound fixed nitrogen to the present invention
Bio-feritlizer and preparation method thereof, it, which can effectively solve existing chemical nitrogen fertilizer, easily causes soil acidification and salination, soil
The problems such as microbial activity declines.
To achieve the above object, the present invention is using following technical scheme：
A kind of compound fixed nitrogen microbial manure, includes following raw material：Fixed nitrogen series bacillus bacterium solution 20~30
Kilogram, 80~double centner of humic acid, 800~900 kilograms of powdered rice hulls, 15~20 kilograms of urea, 20~30 kilograms of Diammonium phosphate (DAP) and
15~20 kilograms of potassium sulfate.
A kind of preparation method of compound fixed nitrogen microbial manure, bacterium solution and humic after fixed nitrogen series bacillus is fermented
Acid, powdered rice hulls, urea, Diammonium phosphate (DAP) and potassium sulfate mixing.
Preferably, following steps are included：
1) expansion of fixed nitrogen series bacillus is numerous：By fixed nitrogen series bacillus, shaken cultivation 36~48 is small in following media
When, 36 grams/L of sucrose, 5 grams/L of tryptone, dusty yeast 11 grams/L, MgSO40.51 gram/L, NaCl 3.5 grams/L, Na2MoO4
Gram/L, FeSO4Gram/L, fixed nitrogen series bacillus containing bacterium number be not less than 108Individual/gram；
2) following ingredients are sufficiently mixed：20~30 kilograms of fixed nitrogen series bacillus bacterium solution, 80~1000 kilograms of humic acid,
800~900 kilograms of powdered rice hulls, 15~20 kilograms of urea, 20~30 kilograms of Diammonium phosphate (DAP), 15~20 kilograms of potassium sulfate.
Preferably, the shaken cultivation is carried out under 30 DEG C of environment.
The present invention has clear advantage and beneficial effect compared with prior art, specifically, by above-mentioned technical proposal
Understand：
The compound fixed nitrogen microbial manure being prepared by using inventive formulation and method is powdery and bottom application type fertilizer
Material, fertilization time are applied initial stage in planting, it is therefore an objective to are provided nutrition needed for plant growth, are promoted plant growth, improve
Chemical fertilizer utilization rate, pollution is reduced, reduces pest and disease damage, strengthen the anti-adversity ability of crops, improve crops fruit product
Matter and yield, play an important roll, and can be promoted the use of in agricultural production.
Embodiment
The present invention discloses a kind of compound fixed nitrogen microbial manure, includes following raw material：Fixed nitrogen series bacillus
20~30 kilograms of bacterium solution, 80~double centner of humic acid, 800~900 kilograms of powdered rice hulls, 15~20 kilograms of urea, Diammonium phosphate (DAP) 20
~30 kilograms and 15~20 kilograms of potassium sulfate.
The present invention also discloses a kind of preparation method of compound fixed nitrogen microbial manure, after fixed nitrogen series bacillus is fermented
Bacterium solution mixes with humic acid, powdered rice hulls, urea, Diammonium phosphate (DAP) and potassium sulfate.Specifically, following steps are included：
1) expansion of fixed nitrogen series bacillus is numerous：By fixed nitrogen series bacillus, shaken cultivation 36~48 is small in following media
When, 36 grams/L of sucrose, 5 grams/L of tryptone, dusty yeast 11 grams/L, MgSO40.51 gram/L, NaCl 3.5 grams/L, Na2MoO4
Gram/L, FeSO4Gram/L, fixed nitrogen series bacillus containing bacterium number be not less than 108Individual/gram；The shaken cultivation is entered under 30 DEG C of environment
OK.
2) following ingredients are sufficiently mixed：20~30 kilograms of fixed nitrogen series bacillus bacterium solution, 80~1000 kilograms of humic acid,
800~900 kilograms of powdered rice hulls, 15~20 kilograms of urea, 20~30 kilograms of Diammonium phosphate (DAP), 15~20 kilograms of potassium sulfate.
With multiple embodiments, the invention will be further described below：
Embodiment 1：
A kind of compound fixed nitrogen microbial manure, includes following raw material：20 kilograms of fixed nitrogen series bacillus bacterium solution,
18 kilograms of humic acid double centner, 805 kilograms of powdered rice hulls, 17 kilograms of urea, 30 kilograms of Diammonium phosphate (DAP) and potassium sulfate.
During preparation, bacterium solution and humic acid, powdered rice hulls, urea, Diammonium phosphate (DAP) and sulphur after fixed nitrogen series bacillus is fermented
Sour potassium mixing.Specifically, following steps are included：
1) expansion of fixed nitrogen series bacillus is numerous：By fixed nitrogen series bacillus in following media shaken cultivation 36 hours,
36 grams/L of sucrose, 5 grams/L of tryptone, dusty yeast 11 grams/L, MgSO40.51 gram/L, NaCl 3.5 grams/L, Na2MoO4Gram/L,
FeSO4Gram/L, fixed nitrogen series bacillus containing bacterium number be not less than 108Individual/gram；The shaken cultivation is carried out under 30 DEG C of environment.
2) following ingredients are sufficiently mixed：20 kilograms of fixed nitrogen series bacillus bacterium solution, humic acid double centner, powdered rice hulls 805
Kilogram, 17 kilograms of urea, 18 kilograms of 30 kilograms of Diammonium phosphate (DAP) and potassium sulfate.
Embodiment 2：A kind of compound fixed nitrogen microbial manure, includes following raw material：Fixed nitrogen series bacillus bacterium
15 kilograms of 25 kilograms of liquid, 85 kilograms of humic acid, 800 kilograms of powdered rice hulls, 15 kilograms of urea, 25 kilograms of Diammonium phosphate (DAP) and potassium sulfate.
During preparation, bacterium solution and humic acid, powdered rice hulls, urea, Diammonium phosphate (DAP) and sulphur after fixed nitrogen series bacillus is fermented
Sour potassium mixing.Specifically, following steps are included：
1) expansion of fixed nitrogen series bacillus is numerous：By fixed nitrogen series bacillus in following media shaken cultivation 40 hours,
36 grams/L of sucrose, 5 grams/L of tryptone, dusty yeast 11 grams/L, MgSO40.51 gram/L, NaCl 3.5 grams/L, Na2MoO4Gram/L,
FeSO4Gram/L, fixed nitrogen series bacillus containing bacterium number be not less than 108Individual/gram；The shaken cultivation is carried out under 30 DEG C of environment.
2) following ingredients are sufficiently mixed：25 kilograms of fixed nitrogen series bacillus bacterium solution, 85 kilograms of humic acid, powdered rice hulls 800
Kilogram, 15 kilograms of urea, 15 kilograms of 25 kilograms of Diammonium phosphate (DAP) and potassium sulfate.
Embodiment 3：
A kind of compound fixed nitrogen microbial manure, includes following raw material：22 kilograms of fixed nitrogen series bacillus bacterium solution,
16 kilograms of 80 kilograms of humic acid, 850 kilograms of powdered rice hulls, 16 kilograms of urea, 26 kilograms of Diammonium phosphate (DAP) and potassium sulfate.
During preparation, bacterium solution and humic acid, powdered rice hulls, urea, Diammonium phosphate (DAP) and sulphur after fixed nitrogen series bacillus is fermented
Sour potassium mixing.Specifically, following steps are included：
1) expansion of fixed nitrogen series bacillus is numerous：By fixed nitrogen series bacillus in following media shaken cultivation 45 hours,
36 grams/L of sucrose, 5 grams/L of tryptone, dusty yeast 11 grams/L, MgSO40.51 gram/L, NaCl 3.5 grams/L, Na2MoO4Gram/L,
FeSO4Gram/L, fixed nitrogen series bacillus containing bacterium number be not less than 108Individual/gram；The shaken cultivation is carried out under 30 DEG C of environment.
2) following ingredients are sufficiently mixed：22 kilograms of fixed nitrogen series bacillus bacterium solution, 80 kilograms of humic acid, powdered rice hulls 850
Kilogram, 16 kilograms of urea, 16 kilograms of 26 kilograms of Diammonium phosphate (DAP) and potassium sulfate.
Embodiment 4：
A kind of compound fixed nitrogen microbial manure, includes following raw material：28 kilograms of fixed nitrogen series bacillus bacterium solution,
19 kilograms of 90 kilograms of humic acid, 900 kilograms of powdered rice hulls, 20 kilograms of urea, 28 kilograms of Diammonium phosphate (DAP) and potassium sulfate.
During preparation, bacterium solution and humic acid, powdered rice hulls, urea, Diammonium phosphate (DAP) and sulphur after fixed nitrogen series bacillus is fermented
Sour potassium mixing.Specifically, following steps are included：
1) expansion of fixed nitrogen series bacillus is numerous：By fixed nitrogen series bacillus in following media shaken cultivation 43 hours,
36 grams/L of sucrose, 5 grams/L of tryptone, dusty yeast 11 grams/L, MgSO40.51 gram/L, NaCl 3.5 grams/L, Na2MoO4Gram/L,
FeSO4Gram/L, fixed nitrogen series bacillus containing bacterium number be not less than 108Individual/gram；The shaken cultivation is carried out under 30 DEG C of environment.
2) following ingredients are sufficiently mixed：28 kilograms of fixed nitrogen series bacillus bacterium solution, 90 kilograms of humic acid, powdered rice hulls 900
Kilogram, 20 kilograms of urea, 19 kilograms of 28 kilograms of Diammonium phosphate (DAP) and potassium sulfate.
Embodiment 5：
A kind of compound fixed nitrogen microbial manure, includes following raw material：30 kilograms of fixed nitrogen series bacillus bacterium solution,
20 kilograms of 95 kilograms of humic acid, 860 kilograms of powdered rice hulls, 18 kilograms of urea, 29 kilograms of Diammonium phosphate (DAP) and potassium sulfate.
During preparation, bacterium solution and humic acid, powdered rice hulls, urea, Diammonium phosphate (DAP) and sulphur after fixed nitrogen series bacillus is fermented
Sour potassium mixing.Specifically, following steps are included：
1) expansion of fixed nitrogen series bacillus is numerous：By fixed nitrogen series bacillus in following media shaken cultivation 48 hours,
36 grams/L of sucrose, 5 grams/L of tryptone, dusty yeast 11 grams/L, MgSO40.51 gram/L, NaCl 3.5 grams/L, Na2MoO4Gram/L,
FeSO4Gram/L, fixed nitrogen series bacillus containing bacterium number be not less than 108Individual/gram；The shaken cultivation is carried out under 30 DEG C of environment.
2) following ingredients are sufficiently mixed：30 kilograms of fixed nitrogen series bacillus bacterium solution, 95 kilograms of humic acid, powdered rice hulls 860
Kilogram, 18 kilograms of urea, 20 kilograms of 29 kilograms of Diammonium phosphate (DAP) and potassium sulfate.
Embodiment 6：
A kind of compound fixed nitrogen microbial manure, includes following raw material：26 kilograms of fixed nitrogen series bacillus bacterium solution,
17 kilograms of 98 kilograms of humic acid, 880 kilograms of powdered rice hulls, 19 kilograms of urea, 20 kilograms of Diammonium phosphate (DAP) and potassium sulfate.
During preparation, bacterium solution and humic acid, powdered rice hulls, urea, Diammonium phosphate (DAP) and sulphur after fixed nitrogen series bacillus is fermented
Sour potassium mixing.Specifically, following steps are included：
1) expansion of fixed nitrogen series bacillus is numerous：By fixed nitrogen series bacillus in following media shaken cultivation 41 hours,
36 grams/L of sucrose, 5 grams/L of tryptone, dusty yeast 11 grams/L, MgSO40.51 gram/L, NaCl 3.5 grams/L, Na2MoO4Gram/L,
FeSO4Gram/L, fixed nitrogen series bacillus containing bacterium number be not less than 108Individual/gram；The shaken cultivation is carried out under 30 DEG C of environment.
2) following ingredients are sufficiently mixed：26 kilograms of fixed nitrogen series bacillus bacterium solution, 98 kilograms of humic acid, powdered rice hulls 880
Kilogram, 19 kilograms of urea, 17 kilograms of 20 kilograms of Diammonium phosphate (DAP) and potassium sulfate.
Compound fixed nitrogen microbial manure made from above-mentioned each implementation is tested below：
2014 and 2015, in Hebei Wuqiao, the compound fixed nitrogen microbial manure prepared using embodiment 1-6, inoculation
Corn (when the field planting of base fertilizer persimmon is done during corn seeding, the dosage use by 50 kgs/acre), the results showed that compound fixed nitrogen microorganism fertilizer
Material is than the volume increase respectively 35%, 38%, 39%, 38%, 40% and 41% without using fertilizer.
The technical principle of the present invention is described above in association with specific embodiment.These descriptions are intended merely to explain the present invention's
Principle, and limiting the scope of the invention can not be construed in any way.Based on explanation herein, the technology of this area
Personnel would not require any inventive effort the other embodiments that can associate the present invention, and these modes are fallen within
Within protection scope of the present invention.
Claims (4)
- A kind of 1. compound fixed nitrogen microbial manure, it is characterised in that：Include following raw material：Fixed nitrogen series bacillus bacterium 20~30 kilograms of liquid, 80~double centner of humic acid, 800~900 kilograms of powdered rice hulls, 15~20 kilograms of urea, Diammonium phosphate (DAP) 20~ 30 kilograms and 15~20 kilograms of potassium sulfate.
- A kind of 2. preparation method of compound fixed nitrogen microbial manure as claimed in claim 1, it is characterised in that：By fixed nitrogen class bud Bacterium solution after spore bacillus fermentation mixes with humic acid, powdered rice hulls, urea, Diammonium phosphate (DAP) and potassium sulfate.
- A kind of 3. preparation method of compound fixed nitrogen microbial manure as claimed in claim 2, it is characterised in that：Include following Step：1) expansion of fixed nitrogen series bacillus is numerous：By fixed nitrogen series bacillus in following media shaken cultivation 36~48 hours, 36 grams/L of sucrose, 5 grams/L of tryptone, dusty yeast 11 grams/L, MgSO40.51 gram/L, NaCl 3.5 grams/L, Na2MoO4Gram/L, FeSO4Gram/L, fixed nitrogen series bacillus containing bacterium number be not less than 108Individual/gram；2) following ingredients are sufficiently mixed：20~30 kilograms of fixed nitrogen series bacillus bacterium solution, 80~1000 kilograms of humic acid, rice husk 800~900 kilograms of powder, 15~20 kilograms of urea, 20~30 kilograms of Diammonium phosphate (DAP), 15~20 kilograms of potassium sulfate.
- A kind of 4. preparation method of compound fixed nitrogen microbial manure as claimed in claim 3, it is characterised in that：The vibration training Support and carried out under 30 DEG C of environment.
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108586054A (en) * | 2018-06-12 | 2018-09-28 | 齐鲁工业大学 | The production method of trace element and actinomyces complex microorganism organic fertilizer in sustained release |
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| MX2014000076A (en) * | 2011-06-30 | 2014-11-10 | Indian Agricultural Council | A novel organic fertilizer of banana pseudostem. |
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- 2016-08-08 CN CN201610644361.9A patent/CN107698300A/en active Pending
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| MX2014000076A (en) * | 2011-06-30 | 2014-11-10 | Indian Agricultural Council | A novel organic fertilizer of banana pseudostem. |
| CN102491848A (en) * | 2011-12-08 | 2012-06-13 | 陈忠慧 | Microbial fertilizer for eliminating continuous cropping obstacle and preparation method thereof |
| CN104151082A (en) * | 2014-08-26 | 2014-11-19 | 中国科学院天津工业生物技术研究所 | Microbial organic-inorganic compound fertilizer and preparation method thereof |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN108586054A (en) * | 2018-06-12 | 2018-09-28 | 齐鲁工业大学 | The production method of trace element and actinomyces complex microorganism organic fertilizer in sustained release |
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Application publication date: 20180216 |