CN107691485A - A kind of pond Chinese herbal medicinal bactericide and its manufacture craft - Google Patents
A kind of pond Chinese herbal medicinal bactericide and its manufacture craft Download PDFInfo
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- CN107691485A CN107691485A CN201710715892.7A CN201710715892A CN107691485A CN 107691485 A CN107691485 A CN 107691485A CN 201710715892 A CN201710715892 A CN 201710715892A CN 107691485 A CN107691485 A CN 107691485A
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- parts
- chloroform
- chinese herbal
- herbal medicinal
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
- A01N65/08—Magnoliopsida [dicotyledons]
- A01N65/20—Fabaceae or Leguminosae [Pea or Legume family], e.g. pea, lentil, soybean, clover, acacia, honey locust, derris or millettia
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- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- General Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Agronomy & Crop Science (AREA)
- Mycology (AREA)
- Plant Pathology (AREA)
- Dentistry (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Environmental Sciences (AREA)
- Natural Medicines & Medicinal Plants (AREA)
- Medicines Containing Plant Substances (AREA)
Abstract
The present invention relates to a kind of pond Chinese herbal medicinal bactericide, including following components（By weight）：80 95 parts of folium artemisiae argyi, 30 40 parts of kuh-seng, 25 35 parts of frutus cnidii, 30 40 parts of folium isatidis, 15 25 parts of cordate houttuynia.The 5 taste Chinese medicine is well mixed, crushing of drying in the shade, adds 1% ethanol solution hydrochloride, filtering, obtain acidic extraction liquid, added chloroform and carry out extracting and demixing, then purified.The present invention is stable, easy to maintain, better to no skin irritation, property, and fish and human body are had no toxic side effect.
Description
Technical field
The present invention relates to a kind of biocide preparation, more particularly to a kind of pond Chinese herbal medicinal bactericide and its manufacture craft.
Background technology
Existing pond sterilization system is essentially all using chemicals, although this chemical bactericide is to pond harmful levels of pathogens
With stronger killing action, but more hazardous drugs residual to fish be present, people often eat these fish and can produced
Chronic hazard, and this chemical bactericide has that excitant, property be not unstable, easy to maintain to human body skin, and production is this
Chemical bactericide manufacture craft is also complex, it is necessary to which special industrial equipment, investment is big, big for environment pollution.
The content of the invention
It is an object of the invention to overcome the deficiencies in the prior art, so as to provide a kind of environment-friendly type, have no toxic side effect, make
Make easy, the pond Chinese herbal medicinal bactericide that cost is low, better.
The present invention is achieved by the following technical programs：
A kind of pond Chinese herbal medicinal bactericide, including following components（By weight）：Folium artemisiae argyi 80-95 parts, kuh-seng 30-40 parts, cnidium monnieri
Sub- 25-35 parts, folium isatidis 30-40 parts, cordate houttuynia 15-25 parts.
A kind of above-mentioned pond Chinese herbal medicinal bactericide manufacture craft, comprises the following steps：
（1）5 described taste Chinese medicines are well mixed by corresponding parts by weight, dried in the shade, are crushed standby.
（2）1% ethanol solution hydrochloride is added in grinding medicinal materials into 1, mixed solution is then obtained, is obtained after filtering residual
Slag and extract solution.
（3）Extract solution in 2 is carried out ethanol is recovered under reduced pressure, then removes insoluble matter with 1% hydrochloric acid solution dissolution filter,
Obtain acidic extraction liquid.
（4）Chloroform is added in the acidic extraction liquid obtained into 3, removes the pigment in acidic extraction liquid, extracting and demixing obtains
To chloroform layer and water layer.
（5）In the water layer obtained into 4 plus sodium carbonate alkalizes to pH=9~10, then adds chloroform and carries out extracting and demixing,
Obtain chloroform layer and water layer.
（6）Chloroform layer in 5 is subjected to recovery chloroform, alkaloid has just been obtained, has then been purified, finally obtains pure production
Product.
Beneficial effects of the present invention：The present invention uses Chinese herbal medicine as raw material, to no skin irritation, property stabilization, Yi Bao
Deposit, be better, fish and human body are had no toxic side effect, and the crude drug source of the present invention is abundant, cost is relatively low, production technology
Simplicity, investment is small, is adapted to large-scale production, in pharmacy procedure effective ingredient be sufficiently reserved, effect it is more preferable.
Embodiment
A kind of pond Chinese herbal medicinal bactericide, including following components（By weight）：Folium artemisiae argyi 80-95 parts, kuh-seng 30-40 parts,
Frutus cnidii 25-35 parts, folium isatidis 30-40 parts, cordate houttuynia 15-25 parts.
A kind of above-mentioned pond Chinese herbal medicinal bactericide manufacture craft, comprises the following steps：
（1）5 described taste Chinese medicines are well mixed by corresponding parts by weight, dried in the shade, are crushed standby.
（2）1% ethanol solution hydrochloride is added in grinding medicinal materials into 1, mixed solution is then obtained, is obtained after filtering residual
Slag and extract solution.
（3）Extract solution in 2 is carried out ethanol is recovered under reduced pressure, then removes insoluble matter with 1% hydrochloric acid solution dissolution filter,
Obtain acidic extraction liquid.
（4）Chloroform is added in the acidic extraction liquid obtained into 3, removes the pigment in acidic extraction liquid, extracting and demixing obtains
To chloroform layer and water layer.
（5）In the water layer obtained into 4 plus sodium carbonate alkalizes to pH=9~10, then adds chloroform and carries out extracting and demixing,
Obtain chloroform layer and water layer.
（6）Chloroform layer is subjected to recovery chloroform, alkaloid has just been obtained, has then been purified, finally obtained net product.
Above-mentioned pond Chinese herbal medicinal bactericide is subjected to bacteriostatic experiment by the following method：
1st, the preparation of mother liquor：With 1mol/L HCl solutions dissolving 20mg ponds Chinese herbal medicinal bactericide refined product, then with 19mL without
Bacterium water dilutes, and PH 1.8, is put under normal temperature, is preserved as mother liquor, standby.It is 6.0,6.5,7.0 and 7.7 to test PH gradients, kind
Solanine concentration gradient is 0.05mg/ml, 0.1 mg/ml, 0.2 mg/ml, 0.4 mg/ml, 0.8 mg/ml and 1.0 mg/ml.
2nd, the preparation of tablet
A diameter of 5mm qualitative filter paper piece is tailored out with puncher, a number of scraps of paper are put in various concentrations extract solution bottle
Middle soaked overnight（12h）, 4 DEG C of preservations are stand-by.
3rd, the culture of common causative
It is standby after 24h is cultivated on culture medium using rubbing method.
4th, drug sensitive test
In desinfection chamber, different pathogenic bacterium solutions are spread evenly across to the culture dish surface of existing culture medium, then gripped with sharp mouth tweezers
Susceptibility piece, it is put in plate, each plate puts 3, and each concentration group does 2 Duplicate Samples, is tested by control group of water, is put in 28
Cultivated in DEG C constant incubator, Bacteria Culture 24h, fungi 48h, measure inhibition zone, it is as a result as follows：
Note：Data=average value ± SD, n=6, unit：mm
When Tablet diameter is 5mm, inhibition zone ＞ 10mm represent that bacteriostasis is strong, and 8-10mm is medium, and ＜ 8mm are weak, above-mentioned medicine
Quick experimental data shows that pond Chinese herbal medicinal bactericide of the present invention is to Myxococcus piscicola, Pseudomonas fluorescens and Aeromonas hydrophila
Fungistatic effect it is weak, it is medium to the fungistatic effect of salmonella, to fungi（Saccharomycete, aspergillus niger and water mo(u)ld）There is stronger suppression
Bacterium acts on.Various concentrations group is because the pond Chinese herbal medicinal bactericide is easy to various pathogen without bacteriostasis during PH7.7
Acid solution is dissolved in, is slightly soluble in alkaline solution.Therefore this pond Chinese herbal medicinal bactericide is in PH7.0, concentration 0.4-0.8mg/
Ml, it can effectively suppress fungi（Saccharomycete, aspergillus niger and water mo(u)ld）Growth, inhibition zone be respectively 11.32 ± 0.58mm,
17.56 ± 1.57mm and 14.06 ± 0.10mm.
The general suitable growth of fish is in neutral or subalkaline water body, often due to a large amount of organic matters are deposited in pond
Faintly acid is showed in, water quality, and pH is mostly between 6.0-7.0, and this is that promoting the use of for this pond Chinese herbal medicinal bactericide provides
Advantage.
Embodiment of the present invention is only the description carried out to the preferred embodiment of the present invention, not to the present invention
Restriction, on the premise of design philosophy of the present invention is not departed from, this area traditional Chinese medicine technology personnel technique according to the invention side
The all variations and modifications that case is made, it all should include within the scope of the present invention.
Claims (2)
1. a kind of pond Chinese herbal medicinal bactericide, including following components（By weight）：Folium artemisiae argyi 80-95 parts, kuh-seng 30-40 parts, snake
Machine tool 25-35 parts, folium isatidis 30-40 parts, cordate houttuynia 15-25 parts.
A kind of 2. manufacture craft of pond Chinese herbal medicinal bactericide according to claim 1, it is characterised in that：It includes following
Step：
（1）5 described taste Chinese medicines are well mixed by corresponding parts by weight, dried in the shade, are crushed standby；
（2）1% ethanol solution hydrochloride is added in grinding medicinal materials into 1, then obtains mixed solution, obtained after filtering residue and
Extract solution；
（3）Extract solution in 2 is carried out ethanol is recovered under reduced pressure, then remove insoluble matter with 1% hydrochloric acid solution dissolution filter, obtain
Acidic extraction liquid；
（4）Chloroform is added in the acidic extraction liquid obtained into 3, removes the pigment in acidic extraction liquid, extracting and demixing obtains chlorine
Imitative layer and water layer；
（5）In the water layer obtained into 4 plus sodium carbonate alkalizes to pH=9~10, then adds chloroform and carries out extracting and demixing, obtains
Chloroform layer and water layer；
（6）Chloroform layer is subjected to recovery chloroform, alkaloid has just been obtained, has then been purified, finally obtained net product.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710715892.7A CN107691485A (en) | 2017-08-21 | 2017-08-21 | A kind of pond Chinese herbal medicinal bactericide and its manufacture craft |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710715892.7A CN107691485A (en) | 2017-08-21 | 2017-08-21 | A kind of pond Chinese herbal medicinal bactericide and its manufacture craft |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN107691485A true CN107691485A (en) | 2018-02-16 |
Family
ID=61170978
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201710715892.7A Pending CN107691485A (en) | 2017-08-21 | 2017-08-21 | A kind of pond Chinese herbal medicinal bactericide and its manufacture craft |
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Citations (7)
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|---|---|---|---|---|
| CN1478537A (en) * | 2002-08-26 | 2004-03-03 | 上海中耀生物科技有限公司 | Chinese medicinal composition for anti-cancer and analgesia, its preparation method and its application in the preparation of product and medicine for treating cancer and analgesia |
| CN102406670A (en) * | 2010-09-21 | 2012-04-11 | 中国人民解放军第三军医大学 | Extraction method of total alkaloids from tripterygium hypoglaucum and application of extract |
| CN103348934A (en) * | 2013-06-07 | 2013-10-16 | 全椒县赤镇龙虾经济专业合作社 | Environment-friendly cultivation method for lobsters |
| CN104365696A (en) * | 2014-09-24 | 2015-02-25 | 湖南农业大学 | Chinese herbal medicinal disinfectant for breeding industry |
| CN105502680A (en) * | 2015-11-25 | 2016-04-20 | 全椒县鮰鱼养殖专业合作社 | Preparation method of Chinese medicinal purifying agent for longsnout catfish ponds |
| CN105613614A (en) * | 2015-12-17 | 2016-06-01 | 集美大学 | Phyteral micro-ecological conditioning agent for reducing pathogenic bacteria in aquaculture and preparation method of phyteral micro-ecological conditioning agent |
| CN106689222A (en) * | 2015-11-13 | 2017-05-24 | 鼎正动物药业（天津）有限公司 | Traditional Chinese medicine disinfectant for preventing aquaculture bacteriosis and preparation method of disinfectant |
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2017
- 2017-08-21 CN CN201710715892.7A patent/CN107691485A/en active Pending
Patent Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1478537A (en) * | 2002-08-26 | 2004-03-03 | 上海中耀生物科技有限公司 | Chinese medicinal composition for anti-cancer and analgesia, its preparation method and its application in the preparation of product and medicine for treating cancer and analgesia |
| CN102406670A (en) * | 2010-09-21 | 2012-04-11 | 中国人民解放军第三军医大学 | Extraction method of total alkaloids from tripterygium hypoglaucum and application of extract |
| CN103348934A (en) * | 2013-06-07 | 2013-10-16 | 全椒县赤镇龙虾经济专业合作社 | Environment-friendly cultivation method for lobsters |
| CN104365696A (en) * | 2014-09-24 | 2015-02-25 | 湖南农业大学 | Chinese herbal medicinal disinfectant for breeding industry |
| CN106689222A (en) * | 2015-11-13 | 2017-05-24 | 鼎正动物药业（天津）有限公司 | Traditional Chinese medicine disinfectant for preventing aquaculture bacteriosis and preparation method of disinfectant |
| CN105502680A (en) * | 2015-11-25 | 2016-04-20 | 全椒县鮰鱼养殖专业合作社 | Preparation method of Chinese medicinal purifying agent for longsnout catfish ponds |
| CN105613614A (en) * | 2015-12-17 | 2016-06-01 | 集美大学 | Phyteral micro-ecological conditioning agent for reducing pathogenic bacteria in aquaculture and preparation method of phyteral micro-ecological conditioning agent |
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| 庞满坤: "《天然药物化学基础》", 31 August 2013, 中国中医药出版社 * |
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Application publication date: 20180216 |
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