CN1076727A - A kind of method with extracting superoxide dismutase from plant - Google Patents
A kind of method with extracting superoxide dismutase from plant Download PDFInfo
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- CN1076727A CN1076727A CN 93111007 CN93111007A CN1076727A CN 1076727 A CN1076727 A CN 1076727A CN 93111007 CN93111007 CN 93111007 CN 93111007 A CN93111007 A CN 93111007A CN 1076727 A CN1076727 A CN 1076727A
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Abstract
A kind of method with extracting superoxide dismutase from plant (SOD); its technical scheme is with the root of plant, stem, leaf, seed, the optional one of fruit; after sterilization; water purification soaks, and is ground into slurry, uses 0.5-10 times protection liquid to extract again; filter then, remove the gred; the SOD crude product, with the SOD crude product make with extra care nutrition stock solution, with crude product and wine liquid allocate the liquid that keeps healthy.Advantage of the present invention is: extract SOD from plant rhizome leaf and seed, fruit, raw material is extensive, and technology is simple, and production cost is low, and quality product is safe and reliable, is fit to produce in enormous quantities, has enlarged use range.
Description
The present invention relates to a kind of preparation method of superoxide dismutase, particularly a kind of method of utilizing the plant extract superoxide dismutase.
Because the existence of free radical causes cell injury or death, make the person produce multiple disease in the human body.And superoxide dismutase (SOD) is in the intravital effect of people, be to eliminate the intravital free radical of people, thereby reduce the generation of disease and delay senility, existing SOD extracts from animal plasma, not only technology is complicated, and the production cost height can not mass production, and pollution, poor stability arranged more.Therefore people's novel method of seeking a kind of SOD of production addressing the above problem, and enlarges its range of application.
The purpose of this invention is to provide a kind of novel method of producing superoxide dismutase, extract from plant rhizome leaf and fruit, seed, raw material is extensive, and technology is simple, and production cost is low, and quality product is safe and reliable, be fit to produce in enormous quantities, and the broadened application scope.
Method with the plant extract superoxide dismutase is as follows: with the optional one of root, stem, leaf, seed, fruit of plant, after sterilization, the raw material that water content is few wants water purification to soak 4-60 hour; be ground into slurry; extract with 0.5-10 protection liquid doubly again, filter then, remove the gred, get the SOD crude product.
The method of extracting SOD crude product producing drink (stoste) with aforesaid method is as follows: the SOD crude product is concentrated, again through oversalting, precipitation, concentrate with the dissolving of protection liquid, upper prop, wash-out, lyophilize get finished product.
The method of extracting SOD crude product production health care liquid with aforesaid method is as follows: with SOD crude product and wine liquid 0.5-2 by weight: the 2-14 compounding, the liquid finished product must keep healthy.
Advantage of the present invention is: extract SOD from plant rhizome leaf and seed, fruit, raw material is extensive, and technology is simple, and production cost is low, and quality product is safe and reliable, is fit to produce in enormous quantities, and produces health-care effect with SOD, has enlarged use range.
Provide embodiments of the invention below:
Produce the method for superoxide dismutase (SOD) crude product:
Embodiment one
Under 15 ℃ of temperature condition, after disinfecting in alcohol, water purification soaked 8 hours, pulverized pulping with corn, extracted with 0.005mol/L, the PH=7.8 phosphoric acid salt of 3 times of weight, and filtering solution adds sulfuric acid amine salt then and analyses, remove the gred the SOD crude product.
Embodiment two
Celery under 10 ℃ of temperature condition, after disinfecting in alcohol, is pulverized pulping, extract with 0.005mol/L, the PH=7.8 phosphoric acid salt of 6 times of weight, filtering solution adds sulfuric acid amine salt then and analyses, remove the gred the SOD crude product.
Embodiment three
Semen Maydis powder slurry with embodiment one extracts with 4 times of weight, 95% ethanolic soln, and other process is identical.
Method with plant extract SOD crude product producing drink (stoste):
Embodiment four
The SOD crude product of the present invention being pressed embodiment one gained concentrates, uses sulfuric acid amine aqueous solution salt precipitation, with the clear liquid upper prop, DEAE-Mierocrystalline cellulose (DE-32), use 0.025mol/L, PH=7.5 potassium phosphate buffer balance in advance, carry out gradient elution with 0.0025-0.2mol/L, PH=7.8 potassium phosphate buffer behind the upper prop, collect finished product, carry out ultrafiltration and concentration at last, lyophilize gets product.
Method with plant extract SOD crude product production health care liquid:
Embodiment five
The present invention is formed the anti-ageing wine of SOD (health care liquid) by the SOD crude product of embodiment three gained and the allotment in 1.5: 10 by weight proportion of wine liquid.
Embodiment six
As basic material, pulping is pulverized in the sterilization back with sorb, and 10 times, 95% by weight then ethanolic soln extracts, filtering solution, add sulfuric acid amine salt then and analyse, remove the gred crude product, last allocated and the anti-ageing wine of achievement type (health care liquid) in 1: 8 by weight proportion with wine liquid.
Embodiment seven
Pepper root as basic material, is pressed embodiment six methods and extracted the SOD crude product, and last and wine liquid allotment in 1.5: 5 by weight proportion forms the anti-ageing wine of plant type (health care liquid).
Embodiment eight
Celery as basic material, is pressed embodiment six methods and extracted the SOD crude product, and last and wine liquid allotment in 2: 11 by weight proportion forms the anti-ageing wine of vegetables type (health care liquid).
Claims (4)
1, a kind of method with plant extract superoxide dismutase (SOD); it is characterized in that: with the optional one of root, stem, leaf, seed, fruit of plant; after sterilization; the raw material that water content is few wants water purification to soak 4-60 hour; be ground into slurry; extract with 0.5-10 protection liquid doubly again, filter then, remove the gred, get the SOD crude product.
2, method according to claim 1 is characterized in that: extracting solution can adopt phosphoric acid salt, ethanol.
3, a kind of method with plant extract SOD crude product producing drink (stoste) is characterized in that: the SOD crude product is concentrated, again through oversalting, precipitation, concentrate with the dissolving of protection liquid, upper prop, wash-out, lyophilize get finished product.
4, a kind of method with plant extract SOD crude product production health care liquid, it is characterized in that: SOD crude product and wine liquid are pressed 0.5-2: the 2-14 compounding gets the health promoting wine finished product.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 93111007 CN1076727A (en) | 1993-03-26 | 1993-03-26 | A kind of method with extracting superoxide dismutase from plant |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 93111007 CN1076727A (en) | 1993-03-26 | 1993-03-26 | A kind of method with extracting superoxide dismutase from plant |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN1076727A true CN1076727A (en) | 1993-09-29 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 93111007 Pending CN1076727A (en) | 1993-03-26 | 1993-03-26 | A kind of method with extracting superoxide dismutase from plant |
Country Status (1)
| Country | Link |
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| CN (1) | CN1076727A (en) |
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN100422318C (en) * | 2006-08-08 | 2008-10-01 | 李健 | Oxygen-enriched catalyst large scale production method of plant erythrocuprein |
| CN100427141C (en) * | 2004-07-19 | 2008-10-22 | 王成余 | Composition of SOD and animal medicine or its extractives, and its prepn. method |
| CN102517264A (en) * | 2011-12-28 | 2012-06-27 | 湖南大学 | Method for extracting superoxide dismutase from plants |
| CN103805575A (en) * | 2014-01-13 | 2014-05-21 | 李健 | Oxygen-enriched and anaerobic alternating catalytic production method of plant superoxide dismutase |
| CN104087562A (en) * | 2014-07-24 | 2014-10-08 | 北京翰若生物科技有限公司 | Extraction method of superoxide dismutase |
| CN107594522A (en) * | 2017-11-03 | 2018-01-19 | 襄阳星马食品有限公司 | The preparation method of the micro- SOD functional food of benefit |
-
1993
- 1993-03-26 CN CN 93111007 patent/CN1076727A/en active Pending
Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN100427141C (en) * | 2004-07-19 | 2008-10-22 | 王成余 | Composition of SOD and animal medicine or its extractives, and its prepn. method |
| CN100422318C (en) * | 2006-08-08 | 2008-10-01 | 李健 | Oxygen-enriched catalyst large scale production method of plant erythrocuprein |
| CN102517264A (en) * | 2011-12-28 | 2012-06-27 | 湖南大学 | Method for extracting superoxide dismutase from plants |
| CN102517264B (en) * | 2011-12-28 | 2013-04-17 | 湖南大学 | Method for extracting superoxide dismutase from plants |
| CN103805575A (en) * | 2014-01-13 | 2014-05-21 | 李健 | Oxygen-enriched and anaerobic alternating catalytic production method of plant superoxide dismutase |
| CN104087562A (en) * | 2014-07-24 | 2014-10-08 | 北京翰若生物科技有限公司 | Extraction method of superoxide dismutase |
| CN104087562B (en) * | 2014-07-24 | 2016-08-24 | 北京翰若生物科技有限公司 | A kind of extracting method of superoxide dismutase |
| CN107594522A (en) * | 2017-11-03 | 2018-01-19 | 襄阳星马食品有限公司 | The preparation method of the micro- SOD functional food of benefit |
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