CN109806286A - The separation method of alkaloid in a kind of leaf of Moringa - Google Patents
The separation method of alkaloid in a kind of leaf of Moringa Download PDFInfo
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- CN109806286A CN109806286A CN201910230354.8A CN201910230354A CN109806286A CN 109806286 A CN109806286 A CN 109806286A CN 201910230354 A CN201910230354 A CN 201910230354A CN 109806286 A CN109806286 A CN 109806286A
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- moringa
- leaf
- alkaloid
- separation method
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Abstract
The present invention relates to a kind of separation methods of alkaloid in leaf of Moringa, it is characterised in that using alkaloid in steady double-aqueous phase system extraction and separation leaf of Moringa.This method can significantly reduce impurity content, and easy to operate, favorable reproducibility, be free of toxic organic solvents, be avoided that environmental pollution, operation is continuous, is easy to amplify.
Description
Technical field
The present invention relates to compound separation methods, and in particular to the separation method of alkaloid in a kind of leaf of Moringa belongs to drug
Technical field.
Background technique
Moringa is Moringaceae Moringa platymiscium, is a kind of tropical deciduous tree for having unique economic to be worth, because it is abnormal rich
Rich and comprehensive nutritional ingredient and active constituent, cause global concern, be known as " 21 century most nutritive value it is pure
Natural plants " and " mother's ace buddy ", " tree of miracle " is become by scientist, " inconceivable tree " is described as by Japan,
" milk of the poor " are described as by Africa, " the human body bodyguard of 21 century " is referred to as by China Taiwan, the west with Chinese ganoderma lucidum and the U.S.
American ginseng is simultaneously known as " world's Triratna ".The place of production of Moringa is located at the northwestward of India, now plants extensively in Asia, Africa and Central America
More than 30 torrid zones, subtropical countries or area, China introduces Moringa at the beginning of the sixties in last century and carries out cultivating and growing, but close several
Year just starts to be researched and developed.
Leaf of Moringa is there are many medicine effectiveness, such as anti-inflammatory is antibacterial, anti-oxidant, hypoglycemic, blood pressure lowering, antitumor, effectively
Ingredient mainly has alkaloid, brass, polysaccharide, water soluble protein etc., and wherein alkaloid is distributed in the content in leaf and is significantly higher than
Stem, total alkaloid are up to 4.62mg/g, and especially pterygospermin has apparent bactericidal effect, can be developed into natural cleaning
Agent and biological pesticide.
Document both at home and abroad about pterygospermin research is fewer and fewer, there is no use about alkaloid in leaf of Moringa at present
The report that two-way extraction isolates and purifies.Alkaloid generally uses water or inorganic acid aqueous solution, such as 0.1%~1% sulfuric acid or salt
Aqueous acid extracts, then is isolated and purified by macroporous absorbent resin, cumbersome, less reproducible, due in leaf of Moringa
Containing water-soluble substances such as a large amount of pigments, protein, polysaccharide, it is bad that effect is isolated and purified using macroporous absorbent resin, and compare
It is cumbersome.We use ethyl alcohol/dipotassium hydrogen phosphate aqueous two-phase extraction using alkaloid in aqueous two-phase system extraction leaf of Moringa, discovery
Alkaloid in leaf of Moringa can significantly reduce impurity content, and extraction yield is up to 96% or more, 1 times higher than conventional isolation and purification method with
On, the stable system, split-phase are fast, and this method is easy to operate, favorable reproducibility, is free of toxic organic solvents, is avoided that environment dirt
Dye, operation is continuous, is easy to amplify.
Summary of the invention
The object of the present invention is to provide a kind of separation methods of alkaloid in leaf of Moringa.It is biological in the leaf of Moringa
The separation method of alkali is as follows: weighing a certain amount of leaf of Moringa, adds 10 times of amount water, extract at microwave power 800W, 90 DEG C of temperature
30min is filtered while hot, and filtrate is leaf of Moringa water extract.Appropriate dipotassium hydrogen phosphate ultrasound is added in leaf of Moringa water extract
Make to dissolve, add a certain amount of ethyl alcohol, and adjust optimum pH, constructs double-aqueous phase system, at 40 DEG C, stirring extraction 10min,
It is transferred in separatory funnel, stratification, takes out upper layer phase and replaces fresh upper phase, carry out multistage cross flow extraction, merge upper liquid,
It is dried under reduced pressure to obtain the final product.
The innovative point of the separation method of alkaloid is using ethyl alcohol/dipotassium hydrogen phosphate aqueous two-phase body in leaf of Moringa of the invention
Alkaloid in system's extraction leaf of Moringa.
Preferably, the double-aqueous phase system is made of dehydrated alcohol and dipotassium hydrogen phosphate.
Preferably, the dehydrated alcohol content ratio is 32%~49%.
Preferably, the dipotassium hydrogen phosphate content ratio is 10%~28%.
Preferably, the optimum pH is 6.8~7.2.
Most preferably, the extraction is 3 grades of cross current solvent extractions.
Specific embodiment
Embodiment 1 weighs leaf of Moringa 2g, adds water 20ml, extracts 30min at microwave power 800W, 90 DEG C of temperature, while hot
Filtering, obtains leaf of Moringa water extract.7g dipotassium hydrogen phosphate ultrasound is added in leaf of Moringa water extract to be made to dissolve, and adds 20ml second
Alcohol, and pH value is adjusted to 7.0, at 40 DEG C, stirring extraction 10min is transferred in separatory funnel, stratification, takes out upper layer phase
And fresh upper phase is replaced, it carries out 3 grades of cross current solvent extractions, merges upper liquid, be dried under reduced pressure to obtain the final product.Measuring extraction yield is 96.98%.
Embodiment 2 weighs leaf of Moringa 2g, adds water 20ml, extracts 30min at microwave power 800W, 90 DEG C of temperature, while hot
Filtering, obtains leaf of Moringa water extract.10g dipotassium hydrogen phosphate ultrasound is added in leaf of Moringa water extract to be made to dissolve, and adds 20ml
Ethyl alcohol, and pH value is adjusted to 6.8, at 40 DEG C, stirring extraction 10min is transferred in separatory funnel, stratification, takes out upper layer
It mutually and replaces fresh upper phase, carries out 2 grades of cross current solvent extractions, merge upper liquid, be dried under reduced pressure to obtain the final product.Measuring extraction yield is 98.60%.
Embodiment 3 weighs leaf of Moringa 2g, adds water 20ml, extracts 30min at microwave power 800W, 90 DEG C of temperature, while hot
Filtering, filtrate is leaf of Moringa water extract.10g dipotassium hydrogen phosphate ultrasound is added in leaf of Moringa water extract to be made to dissolve, then plus
Enter 25ml ethyl alcohol, and adjust pH value to 7.0, at 40 DEG C, stirring extraction 10min is transferred in separatory funnel, stratification takes
It upper layer phase and replaces fresh upper phase out, carries out 3 grades of cross current solvent extractions, merge upper liquid, be dried under reduced pressure to obtain the final product.Measuring extraction yield is
96.31%。
Embodiment 4 weighs leaf of Moringa 1g, adds water 10ml, extracts 30min at microwave power 800W, 90 DEG C of temperature, while hot
Filtering, filtrate is leaf of Moringa water extract.5g dipotassium hydrogen phosphate ultrasound is added in leaf of Moringa water extract to be made to dissolve, and is added
12ml ethyl alcohol, and pH value is adjusted to 7.2, at 40 DEG C, stirring extraction 10min is transferred in separatory funnel, stratification, takes out
Upper layer phase simultaneously replaces fresh upper phase, carries out 2 grades of cross current solvent extractions, merges upper liquid, is dried under reduced pressure to obtain the final product.Measuring extraction yield is
96.07%。
Embodiment 5 weighs leaf of Moringa 1g, adds water 10ml, extracts 30min at microwave power 800W, 90 DEG C of temperature, while hot
Filtering, filtrate is leaf of Moringa water extract.4g dipotassium hydrogen phosphate ultrasound is added in leaf of Moringa water extract to be made to dissolve, and is added
10ml ethyl alcohol, and pH value is adjusted to 7.0, at 40 DEG C, stirring extraction 10min is transferred in separatory funnel, stratification, takes out
Upper layer phase simultaneously replaces fresh upper phase, carries out 3 grades of cross current solvent extractions, merges upper liquid, is dried under reduced pressure to obtain the final product.Measuring extraction yield is
97.12%。
Claims (6)
1. the separation method of alkaloid in a kind of leaf of Moringa, its step are as follows: a certain amount of leaf of Moringa is weighed, 10 times of amount water are added,
Microwave power 800W extracts 30min at 90 DEG C of temperature, filters while hot, filtrate is leaf of Moringa water extract.It is mentioned in leaf of Moringa water
Appropriate dipotassium hydrogen phosphate ultrasound is added in object to be made to dissolve, and adds a certain amount of ethyl alcohol, and adjust optimum pH, constructs aqueous two-phase body
System, at 40 DEG C, stirring extraction 10min is transferred in separatory funnel, stratification, takes out upper layer phase and replace fresh upper phase, into
The extraction of row multistage cross flow, merges upper liquid, is dried under reduced pressure to obtain the final product.
2. the separation method of alkaloid in leaf of Moringa as described in claim 1, which is characterized in that double-aqueous phase system is by anhydrous second
Pure and mild dipotassium hydrogen phosphate composition.
3. the separation method of alkaloid in leaf of Moringa as described in claim 1, which is characterized in that dehydrated alcohol content ratio is
32%~49%.
4. the separation method of alkaloid in leaf of Moringa as described in claim 1, which is characterized in that dipotassium hydrogen phosphate content ratio
It is 10%~28%.
5. the separation method of alkaloid in leaf of Moringa as described in claim 1, which is characterized in that optimum pH be 6.8~
7.2。
6. the separation method of alkaloid in leaf of Moringa as described in claim 1, which is characterized in that extract as 3 grades of cross current solvent extractions.
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| CN201910230354.8A CN109806286A (en) | 2019-03-26 | 2019-03-26 | The separation method of alkaloid in a kind of leaf of Moringa |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111217864A (en) * | 2020-02-28 | 2020-06-02 | 营山椒宝宝花椒有限责任公司 | Extraction method of green pepper alkaloid |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN101050212A (en) * | 2007-05-18 | 2007-10-10 | 中南大学 | Method for extracting high pure natural nicotine and solanesol at same time from waste and low-grade tobacco |
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2019
- 2019-03-26 CN CN201910230354.8A patent/CN109806286A/en active Pending
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101050212A (en) * | 2007-05-18 | 2007-10-10 | 中南大学 | Method for extracting high pure natural nicotine and solanesol at same time from waste and low-grade tobacco |
Non-Patent Citations (2)
| Title |
|---|
| 徐小龙: "马钱子总生物碱的提取工艺研究", 《中国优秀硕士学位论文全文数据库 工程科技Ⅰ辑》 * |
| 罗晓波等: "辣木叶的价值及其开发利用研究进展", 《资源开发与市场》 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111217864A (en) * | 2020-02-28 | 2020-06-02 | 营山椒宝宝花椒有限责任公司 | Extraction method of green pepper alkaloid |
| CN111217864B (en) * | 2020-02-28 | 2020-10-27 | 营山椒宝宝花椒有限责任公司 | Extraction method of green pepper alkaloid |
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Application publication date: 20190528 |