CN107669944B - Traditional Chinese medicine composition for treating blood stasis type anxiety disorder and preparation method thereof - Google Patents
Traditional Chinese medicine composition for treating blood stasis type anxiety disorder and preparation method thereof Download PDFInfo
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- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/88—Liliopsida (monocotyledons)
- A61K36/899—Poaceae or Gramineae (Grass family), e.g. bamboo, corn or sugar cane
- A61K36/8998—Hordeum (barley)
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- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/06—Fungi, e.g. yeasts
- A61K36/07—Basidiomycota, e.g. Cryptococcus
- A61K36/076—Poria
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- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/23—Apiaceae or Umbelliferae (Carrot family), e.g. dill, chervil, coriander or cumin
- A61K36/232—Angelica
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- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/23—Apiaceae or Umbelliferae (Carrot family), e.g. dill, chervil, coriander or cumin
- A61K36/233—Bupleurum
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- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/28—Asteraceae or Compositae (Aster or Sunflower family), e.g. chamomile, feverfew, yarrow or echinacea
- A61K36/286—Carthamus (distaff thistle)
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- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/53—Lamiaceae or Labiatae (Mint family), e.g. thyme, rosemary or lavender
- A61K36/537—Salvia (sage)
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- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/65—Paeoniaceae (Peony family), e.g. Chinese peony
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- A61K36/736—Prunus, e.g. plum, cherry, peach, apricot or almond
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- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/80—Scrophulariaceae (Figwort family)
- A61K36/804—Rehmannia
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
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- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/88—Liliopsida (monocotyledons)
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- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/30—Extraction of the material
- A61K2236/37—Extraction at elevated pressure or temperature, e.g. pressurized solvent extraction [PSE], supercritical carbon dioxide extraction or subcritical water extraction
Abstract
The invention discloses a traditional Chinese medicine composition for treating blood stasis type anxiety disorder and a preparation method thereof, and the traditional Chinese medicine composition comprises, by weight, 5-15 parts of Chinese angelica, 7-17 parts of salvia miltiorrhiza, 7-17 parts of dried rehmannia root, 5-15 parts of red peony root, 5-15 parts of peach seed, 5-15 parts of safflower, 5-15 parts of radix bupleuri, 5-15 parts of rhizoma cyperi, 5-15 parts of raw malt, 5-15 parts of poria with hostwood, 5-15 parts of calamus and 18-32 parts of dens draconis. The invention adopts supercritical fluid extraction technology and applies supercritical CO2Is a solvent, so that the extraction rate and the purity of the effective components are obviously improved, the method can be widely used for producing medicines of various formulations, the prepared finished product has less dosage, the drug effect takes effect quickly, and the drug effect lasts for a long time.
Description
Technical Field
The patent belongs to the field of traditional Chinese medicine, in particular relates to a traditional Chinese medicine composition and a preparation method thereof, and in particular relates to a traditional Chinese medicine composition for treating blood stasis type anxiety disorder and a preparation method thereof.
Background
Anxiety, as its name suggests, is restlessness and restlessness. Excessive, it means that excessive factors are considered. In view of a series of syndromes, they belong to the category of "depression". Generally speaking, it is damaged by seven emotions, which causes disorder of qi activity of five zang organs. So the term "Ling Shu & Kou Wen chapter" means that sorrow and worries cause heart attack, and heart attack will affect the heart because of stagnation of liver qi and failure to reach the heart; "Su Wen & Ling lan Mi Dian Lun" considers that the heart, monarch organ, spirit are so much; the book of Su Wen and the six sections Tibetan elephants treatise is characterized in that: the heart, the root of life and the spirit also change. "the ancient medical works describe enough to indicate that the disease is a mental disease. The traditional Chinese medicine considers that' blood is the mother of qi, qi is the commander of blood, qi circulation is blood circulation, and qi stagnation is blood stagnation. The main treatment of the disease is mainly to remove blood stasis, soothe liver and relieve depression, promote qi circulation and slightly relieve the mind and tranquilize the mind when the heart is affected by blood stasis and qi stagnation. So that the heart spirit can nourish the mind, the mind can regulate the flow of qi, and a series of symptoms of anxiety can disappear.
The disease is clinically manifested by emotional restlessness symptoms such as emotional tension, restlessness, easy fright and fear. According to clinical manifestations, it should be classified into palpitations, depression and heart diseases. The traditional Chinese medicine considers that: the disease is mainly caused by deficiency of qi and blood, emotional internal injury, depression of liver qi, dysfunction of qi regulation, disorder of qi movement, dysfunction of ascending and descending, dysfunction of spleen in transportation, deficiency of biochemical and healthy transportation, gradual deficiency of qi and blood, failure of heart spirit, internal resistance of phlegm stasis, gallbladder deficiency, failure of function, qi stagnation transforming into fire, long-term heart injury, disturbance of heart spirit, restlessness and no dominance of mind.
The patients are generally recommended to take tranquilizers and antidepressants for 1-2 years by taking the factors such as the illness state, physical condition and economic condition of the patients into consideration. For example, the effect duration of the diazepam western medicine is short, the diazepam western medicine is not suitable for long-term use in large quantities, and the medicine dependence can be generated; the purity of common traditional Chinese medicines is low, the anxiolytic effect is slow, and other tranquilizers need to be used together for a short time. By combining the analysis and the current market demand, the traditional Chinese medicine for treating the anxiety disorder, which has high safety, quick response, long duration and no dependence, is urgently needed.
Content of patent
Aiming at the defects, the invention provides a traditional Chinese medicine composition for treating blood stasis type anxiety disorder and a preparation method thereof, wherein natural Chinese herbal medicines such as angelica, salvia miltiorrhiza, radix rehmanniae, red paeony root, peach kernel, safflower, radix bupleuri, rhizoma cyperi, raw malt, poria with hostwood, calamus, dens draconis and the like are selected and prepared into traditional Chinese medicine products with different dosage forms by adopting a proper preparation method. The prepared traditional Chinese medicine product can effectively treat blood stasis type anxiety disorder through the synergistic promotion effect of the effective components in the traditional Chinese medicinal materials, and the finished product has the advantages of quick response, stable effect, small toxic and side effect and convenient taking.
The above object of the present invention is achieved by the following means.
A traditional Chinese medicine composition for treating blood stasis type anxiety disorder comprises the following components in parts by weight: 5-15 parts of angelica sinensis, 7-17 parts of salvia miltiorrhiza, 7-17 parts of radix rehmanniae, 5-15 parts of red peony root, 5-15 parts of peach kernel, 5-15 parts of safflower, 5-15 parts of radix bupleuri, 5-15 parts of rhizoma cyperi, 5-15 parts of raw malt, 5-15 parts of poria with hostwood, 5-15 parts of calamus and 18-32 parts of dens draconis.
Preferably, the traditional Chinese medicine composition for treating blood stasis type anxiety disorder comprises the following components in parts by weight: 8-12 parts of angelica sinensis, 10-14 parts of salvia miltiorrhiza, 10-14 parts of radix rehmanniae, 8-12 parts of radix paeoniae rubra, 8-12 parts of peach kernel, 8-12 parts of safflower, 8-12 parts of radix bupleuri, 8-12 parts of rhizoma cyperi, 8-12 parts of raw malt, 8-12 parts of poria with hostwood, 8-12 parts of calamus and 23-27 parts of dens draconis.
More preferably, the traditional Chinese medicine composition for treating blood stasis type anxiety disorder comprises the following components in parts by weight: 10 parts of angelica sinensis, 12 parts of salvia miltiorrhiza, 12 parts of radix rehmanniae, 10 parts of red peony root, 10 parts of peach kernel, 10 parts of safflower, 10 parts of radix bupleuri, 10 parts of rhizoma cyperi, 10 parts of raw malt, 10 parts of poria with hostwood, 10 parts of calamus and 25 parts of dens draconis.
In the components of the traditional Chinese medicine composition, the angelica can enrich blood and activate blood, regulate menstruation and relieve pain, moisten intestines and relieve constipation, the salvia can remove blood stasis and relieve pain, activate blood and dredge channels, clear away the heart fire and relieve restlessness, the rehmannia root can clear away heat and promote the production of body fluid, moisten dryness and smooth intestines, break blood stasis and activate blood, the red peony root can clear away heat and cool blood, activate blood and remove blood stasis, the peach kernel can activate blood and remove blood stasis and relax bowel, and the safflower can activate blood and activate meridians, remove blood stasis and relieve pain, which are; radix bupleuri can relieve exterior syndrome, raise yang, soothe liver and relieve depression, rhizoma cyperi can sooth liver and relieve depression, regulate qi and relieve epigastric distention, regulate menstruation and relieve pain, raw malt can promote qi circulation and promote digestion, invigorate spleen and stimulate appetite, poria with hostwood can calm heart, calm nerves and promote diuresis, calamus can resuscitate and eliminate phlegm, regulate qi and invigorate blood circulation, dispel wind and remove dampness, and dens draconis can relieve convulsion and calm nerves and relieve restlessness and fever, and the medicines are adjuvant medicines.
The invention also provides a preparation method of the traditional Chinese medicine composition for treating blood stasis type anxiety disorder, which comprises the following steps:
s1, weighing the raw materials including Chinese angelica, salvia miltiorrhiza, radix rehmanniae, red paeony root, peach kernel, safflower, radix bupleuri, rhizoma cyperi, raw malt, poria with hostwood, calamus and dens draconis according to parts by weight, crushing, and sieving by a 40-80-mesh sieve to prepare traditional Chinese medicine powder for later use;
s2 extracting the Chinese medicinal powder with supercritical CO as solvent, S12And (3) extracting at the temperature of 55-65 ℃ for 1-2 h under the extraction pressure of 25-35 MPa, and recovering to obtain an extract product.
Preferably, step S1 is to weigh the raw materials according to parts by weight, grind and then pass through a 60-mesh sieve to obtain the traditional Chinese medicine powder for later use.
Preferably, the extraction temperature of the supercritical fluid extraction in step S2 is 60 ℃.
Preferably, the extraction pressure of the supercritical fluid extraction in step S2 is 30 MPa.
Compared with the prior art, this patent beneficial effect lies in: provides a Chinese medicine for curing blood stasis type anxiety neurosisThe Chinese medicinal composition and the preparation method thereof are characterized in that the Chinese medicinal formula is formed by selecting medicinal materials with better medicinal effect according to a scientific proportion; the extract of the traditional Chinese medicine composition is prepared by adopting a supercritical fluid extraction process, the technology is very advanced in the field of preparation of the extract of the traditional Chinese medicine composition, and supercritical CO is applied2The extract of the prepared traditional Chinese medicine composition can be used for producing medicines of various formulations, and has higher content of effective components, better efficacy and longer duration of efficacy.
Detailed Description
In order to make the objects, technical solutions and advantages of the present invention more apparent, the present invention is further described in detail with reference to the following embodiments. It should be understood that the specific embodiments described herein are merely illustrative of the invention and are not intended to limit the invention. Reagents, methods and apparatus used in the present invention are conventional in the art unless otherwise indicated.
Example 1
A Chinese medicinal composition for treating blood stasis type anxiety comprises radix Angelicae sinensis 5g, Saviae Miltiorrhizae radix 7g, radix rehmanniae 7g, radix Paeoniae Rubra 15g, semen Persicae 15g, Carthami flos 5g, bupleuri radix 5g, rhizoma Cyperi 15g, folium Hordei Germinatus 15g, Poria 5g, rhizoma Acori Calami 15g, and dens Draconis 32 g.
The preparation method of the extract of the traditional Chinese medicine composition comprises the following steps:
s1, weighing the raw materials including Chinese angelica, salvia miltiorrhiza, radix rehmanniae, red paeony root, peach kernel, safflower, radix bupleuri, rhizoma cyperi, raw malt, poria with hostwood, calamus and dens draconis according to parts by weight, crushing, and sieving by a 60-mesh sieve to prepare traditional Chinese medicine powder for later use;
s2 extracting the Chinese medicinal powder with supercritical CO as solvent, S12And the extraction temperature is 60 ℃, the extraction time is 1h, the extraction pressure is 30MPa, and the extract product is obtained by recycling.
Example 2
This example is the same as the production method of example 1 except for the following points.
A Chinese medicinal composition for treating blood stasis type anxiety comprises radix Angelicae sinensis 15g, Saviae Miltiorrhizae radix 17g, radix rehmanniae 17g, radix Paeoniae Rubra 5g, semen Persicae 5g, Carthami flos 15g, bupleuri radix 15g, rhizoma Cyperi 5g, folium Hordei Germinatus 5g, Poria 15g, rhizoma Acori Calami 5g, and dens Draconis 18 g.
Example 3
This example is the same as the production method of example 1 except for the following points.
A Chinese medicinal composition for treating blood stasis type anxiety comprises radix Angelicae sinensis 8g, Saviae Miltiorrhizae radix 10g, radix rehmanniae 10g, radix Paeoniae Rubra 8g, semen Persicae 12g, Carthami flos 12g, bupleuri radix 8g, rhizoma Cyperi 8g, folium Hordei Germinatus 12g, Poria 12g, rhizoma Acori Calami 8g, and dens Draconis 23 g.
Example 4
This example is the same as the production method of example 1 except for the following points.
A Chinese medicinal composition for treating blood stasis type anxiety comprises radix Angelicae sinensis 12g, Saviae Miltiorrhizae radix 14g, radix rehmanniae 14g, radix Paeoniae Rubra 12g, semen Persicae 8g, Carthami flos 8g, bupleuri radix 12g, rhizoma Cyperi 12g, folium Hordei Germinatus 8g, Poria 8g, rhizoma Acori Calami 12g, and dens Draconis 27 g.
Example 5
This example is the same as the production method of example 1 except for the following points.
A traditional Chinese medicine composition for treating blood stasis type anxiety disorder comprises 10g of Chinese angelica, 12g of salvia miltiorrhiza, 12g of radix rehmanniae, 10g of red peony root, 10g of peach kernel, 10g of safflower, 10g of radix bupleuri, 10g of rhizoma cyperi, 10g of raw malt, 10g of poria with hostwood, 10g of calamus and 25g of dens draconis.
Example 6
This example is the same formulation as example 1, except for the following.
Step S1 is: weighing raw materials including Chinese angelica, salvia miltiorrhiza, radix rehmanniae, red paeony root, peach kernel, safflower, radix bupleuri, rhizoma cyperi, raw malt, poria with hostwood, calamus and dens draconis according to parts by weight, crushing, and sieving by a 40-mesh sieve to prepare traditional Chinese medicine powder for later use.
Example 7
This example is the same formulation as example 1, except for the following.
Step S1 is: weighing raw materials including Chinese angelica, salvia miltiorrhiza, radix rehmanniae, red paeony root, peach kernel, safflower, radix bupleuri, rhizoma cyperi, raw malt, poria with hostwood, calamus and dens draconis according to parts by weight, crushing, and sieving with a 80-mesh sieve to prepare traditional Chinese medicine powder for later use.
Example 8
This example is the same formulation as example 1, except for the following.
Step S2 is: and (3) performing supercritical fluid extraction on the Chinese medicinal powder prepared in the step S1 at the extraction temperature of 55 ℃ for 1h and the extraction pressure of 30MPa, and recovering to obtain an extract product.
Example 9
This example is the same formulation as example 1, except for the following.
Step S2 is: and (3) performing supercritical fluid extraction on the Chinese medicinal powder prepared in the step S1 at the extraction temperature of 65 ℃ for 1h and the extraction pressure of 30MPa, and recovering to obtain an extract product.
Example 10
This example is the same formulation as example 1, except for the following.
Step S2 is: and (3) performing supercritical fluid extraction on the Chinese medicinal powder prepared in the step S1 at the extraction temperature of 60 ℃ for 1h and the extraction pressure of 25MPa, and recovering to obtain an extract product.
Example 11
This example is the same formulation as example 1, except for the following.
Step S2 is: and (3) performing supercritical fluid extraction on the Chinese medicinal powder prepared in the step S1 at the extraction temperature of 60 ℃ for 1h and the extraction pressure of 35MPa, and recovering to obtain an extract product.
Comparative example 1
This comparative example was the same formulation as example 1, except for the following points.
A Chinese medicinal composition for treating blood stasis type anxiety comprises radix Angelicae sinensis 4g, Saviae Miltiorrhizae radix 6g, radix rehmanniae 6g, radix Paeoniae Rubra 4g, semen Persicae 4g, Carthami flos 18g, bupleuri radix 18g, rhizoma Cyperi 18g, folium Hordei Germinatus 3g, Poria 18g, rhizoma Acori Calami 18g, and dens Draconis 16 g.
Comparative example 2
This comparative example was the same formulation as example 1, except for the following points.
A Chinese medicinal composition for treating blood stasis type anxiety comprises 17g of radix Angelicae sinensis, 19g of Saviae Miltiorrhizae radix, 19g of radix rehmanniae, 17g of radix Paeoniae Rubra, 17g of semen Persicae, 4g of Carthami flos, 4g of bupleuri radix, 4g of rhizoma Cyperi, 17g of fructus Hordei Germinatus, 3g of Poria, 3g of rhizoma Acori Calami, and 34g of dens Draconis.
Comparative example 3
This comparative example was the same formulation as example 1, except for the following points.
Step S1 is: weighing raw materials including Chinese angelica, salvia miltiorrhiza, radix rehmanniae, red paeony root, peach kernel, safflower, radix bupleuri, rhizoma cyperi, raw malt, poria with hostwood, calamus and dens draconis according to parts by weight, crushing, and sieving by a 30-mesh sieve to prepare traditional Chinese medicine powder for later use.
Comparative example 4
This comparative example was the same formulation as example 1, except for the following points.
Step S1 is: weighing raw materials including Chinese angelica, salvia miltiorrhiza, radix rehmanniae, red paeony root, peach kernel, safflower, radix bupleuri, rhizoma cyperi, raw malt, poria with hostwood, calamus and dens draconis according to parts by weight, crushing, and sieving by a 90-mesh sieve to prepare traditional Chinese medicine powder for later use.
Comparative example 5
This comparative example was the same formulation as example 1, except for the following points.
Step S2 is: and (3) performing supercritical fluid extraction on the Chinese medicinal powder prepared in the step S1 at the extraction temperature of 50 ℃ for 1h and the extraction pressure of 25MPa, and recovering to obtain an extract product.
Comparative example 6
This comparative example was the same formulation as example 1, except for the following points.
Step S2 is: and (3) performing supercritical fluid extraction on the Chinese medicinal powder prepared in the step S1 at the extraction temperature of 70 ℃, the extraction time of 1h and the extraction pressure of 25MPa, and recovering to obtain an extract product.
Comparative example 7
This comparative example was the same formulation as example 1, except for the following points.
Step S2 is: and (3) performing supercritical fluid extraction on the Chinese medicinal powder prepared in the step S1 at the extraction temperature of 60 ℃ for 1h and the extraction pressure of 20MPa, and recovering to obtain an extract product.
Comparative example 8
This comparative example was the same formulation as example 1, except for the following points.
Step S2 is: and (3) performing supercritical fluid extraction on the Chinese medicinal powder prepared in the step S1 at the extraction temperature of 60 ℃ for 1h and the extraction pressure of 40MPa, and recovering to obtain an extract product.
Application example 1
The extracts of the traditional Chinese medicine compositions of examples 1 to 11 and comparative examples 1 to 8 were separated and weighed by combining a single variable method, the extraction rates were calculated, the results obtained were examples 1 to 11 and comparative examples 1 to 8, respectively, and the influence of the formulation composition, the pulverization particle size, the extraction temperature, and the extraction pressure on the extraction rate was judged. The extraction rate is the weight of the extract multiplied by 100 percent per the total weight of the traditional Chinese medicinal materials. Specific results are shown in table 1 below.
TABLE 1 summary of the results of the extraction rates of the examples and comparative examples
In the above data, the extraction rates of examples 1 to 5 are higher than those of examples 1 to 5, wherein the extraction rate of example 5 is the highest, and the extraction rates of examples 3 and 4 times are lower, so the formulations of examples 1 to 5 are better, and the formulation of example 5 is the best; comparing examples 1, 6 and 7 with comparative examples 3 and 4, it is found that the extraction rate of example 1 is slightly higher than examples 6 and 7, and much higher than comparative examples 3 and 4, so the particle size of the traditional Chinese medicine raw materials of examples 1, 6 and 7 is better, and the particle size of the traditional Chinese medicine raw materials of example 1 is optimal; comparing examples 1, 8 and 9 with comparative examples 5 and 6, the extraction rate of example 1 is slightly higher than examples 8 and 9, and is much higher than comparative examples 5 and 6, so the extraction temperature of examples 1, 8 and 9 is better, and the extraction temperature of example 1 is the best; comparing examples 1, 10 and 11 with comparative examples 7 and 8, it can be seen that the extraction rate of example 1 is slightly higher than that of examples 10 and 11, and is much higher than that of comparative examples 7 and 8, so the extraction pressure of examples 1, 10 and 11 is better, and the extraction pressure of example 1 is the best.
Application example 2
The anti-anxiety model test of the traditional Chinese medicine composition is carried out by adopting a mouse tail suspension method.
1. And (3) inspecting materials: SD male Kunming rat, body weight 200 + -20 g; male Kunming mice, body weight 20 + -2 g.
2. Test set
(1) Rat light and dark box test
80 SD rats were randomly selected and randomly divided into A, B, C, D, E implementation groups, A, B comparison groups, and a blank control group. Each rat in groups A to E was fed the extract of the Chinese medicinal composition of examples 1 to 5 in an amount of 0.4ml/2g, each rat in group A, B was fed the extract of the Chinese medicinal composition of comparative examples 1 and 2 in an amount of 0.4ml/2g, and each rat in the blank control group was fed physiological saline in an amount of 0.4ml/2 g. Before the medicine is fed, the rats in each group are placed in the center of a bright room of a shuttle device of a bright and dark box, the number of times that the rats pass through the bright and dark box as a circular hole of a middle clapboard within 15min is observed, and then 11: 00 feeding the rats in each group on time for 8 days, and observing the times of the rats passing through the circular holes of the middle partition plate of the light and dark box within 15min after feeding the rats for 2 hours on the 3 rd, 5 th and 8 th days.
(2) Mouse ladder test
Another 80 mice were randomly selected and randomly divided into groups a, b, c, d, and e, and groups a and b and a blank control group were compared. Each mouse in the groups a to e was fed with the extract of the Chinese medicinal composition of examples 1 to 5 in an amount of 0.4ml/2g, each mouse in the groups a and b was fed with the extract of the Chinese medicinal composition of comparative examples 1 and 2 in an amount of 0.4ml/2g, and each mouse in the blank control was fed with physiological saline in an amount of 0.4ml/2 g. Before the medicine is fed, the mouse is placed at the bottom of the ladder, the ladder is back to the ladder, the number of steps for the mouse to climb over the ladder and the number of times of standing within 10min are observed, and then 11: 00 Each group of mice is fed with the medicine on time for 8 days, and the number of steps of climbing and standing of the mice within 10min is observed after the medicine is fed for 2 hours on the 3 rd, 5 th and 8 th days.
3. Test results
The influence of the extract of the traditional Chinese medicine composition on the number of times that a rat passes through a light box and a dark box and the influence on the number of climbing stairs and the number of standing times of a mouse are shown in the following tables 2 and 3.
TABLE 2 summary of the number of times the rat passes through the light and dark box
| Group name | Before administration | Day 3 | Day 5 | Day 8 |
| Practice group a | 21.5±16.8 | 42.1±53.2* | 56.4±11.7 | 65.8±34.8** |
| Practice group b | 23.8±17.6 | 43.4±28.5* | 57.3±23.9* | 67.6±29.5* |
| Implementation of group c | 22.7±13.8 | 45.2±12.9** | 58.8±13.7** | 67.7±16.5** |
| Implementation of group d | 23.1±21.7 | 46.3±19.7* | 57.9±11.2** | 69.2±12.3** |
| Implementation of group e | 22.8±13.7 | 50.3±27.5** | 63.8±25.2** | 72.7±14.8** |
| Compare group a | 24.1±24.2 | 34.4±39.7** | 43.3±21.3** | 51.8±22.7** |
| Compare group b | 21.4±18.8 | 32.5±41.8* | 45.7±16.7** | 53.9±17.6** |
| Blank control group | 23.9±17.8 | 25.5±18.7 | 27.2±14.9 | 27.7±19.2 |
Note: p < 0.05, P < 0.01, compared to the blank control.
TABLE 3 mouse ladder progression and standing times summary table
Note: p < 0.05, P < 0.01, compared to the blank control.
The data result shows that the extract of the traditional Chinese medicine composition can obviously increase the times of passing through light and dark boxes by rats, increase the ladder climbing number of the mice, reduce the standing times of the mice and has the function of antianxiety. Of these, examples 1 to 5 were significantly more effective than comparative examples 1 and 2, and example 5 had the best antidepressant effect.
The above examples and comparative examples describe the practice of the present invention in detail, but the present patent is not limited to the specific details in the above embodiments. Within the scope of the claims and the technical idea of this patent, a number of simple modifications and changes can be made to the technical solution of this patent, all of which fall within the scope of protection of this patent.
Claims (6)
1. The traditional Chinese medicine composition for treating blood stasis type anxiety disorder is characterized by being prepared from the following components in parts by weight: 5-15 parts of angelica sinensis, 7-17 parts of salvia miltiorrhiza, 7-17 parts of radix rehmanniae, 5-15 parts of red peony root, 5-15 parts of peach kernel, 5-15 parts of safflower, 5-15 parts of radix bupleuri, 5-15 parts of rhizoma cyperi, 5-15 parts of raw malt, 5-15 parts of poria with hostwood, 5-15 parts of calamus and 18-32 parts of dens draconis;
the preparation method comprises the following steps:
s1, weighing the raw materials including Chinese angelica, salvia miltiorrhiza, radix rehmanniae, red paeony root, peach kernel, safflower, radix bupleuri, rhizoma cyperi, raw malt, poria with hostwood, calamus and dens draconis according to parts by weight, crushing, and sieving by a 40-80-mesh sieve to prepare traditional Chinese medicine powder for later use;
and S2, performing supercritical fluid extraction on the Chinese medicinal powder prepared in the S1 at the extraction temperature of 55-65 ℃ for 1-2 h under the extraction pressure of 25-35 MPa, and recovering to obtain an extract product.
2. The traditional Chinese medicine composition for treating blood stasis type anxiety disorder as claimed in claim 1, which is prepared from the following components in parts by weight: 8-12 parts of angelica sinensis, 10-14 parts of salvia miltiorrhiza, 10-14 parts of radix rehmanniae, 8-12 parts of radix paeoniae rubra, 8-12 parts of peach kernel, 8-12 parts of safflower, 8-12 parts of radix bupleuri, 8-12 parts of rhizoma cyperi, 8-12 parts of raw malt, 8-12 parts of poria with hostwood, 8-12 parts of calamus and 23-27 parts of dens draconis.
3. The traditional Chinese medicine composition for treating blood stasis type anxiety disorder as claimed in claim 2, which is prepared from the following components in parts by weight: 10 parts of angelica sinensis, 12 parts of salvia miltiorrhiza, 12 parts of radix rehmanniae, 10 parts of red peony root, 10 parts of peach kernel, 10 parts of safflower, 10 parts of radix bupleuri, 10 parts of rhizoma cyperi, 10 parts of raw malt, 10 parts of poria with hostwood, 10 parts of calamus and 25 parts of dens draconis.
4. The traditional Chinese medicine composition for treating blood stasis type anxiety disorder according to claim 1, wherein step S1 is to weigh the raw traditional Chinese medicines according to parts by weight, grind and then pass through a 60-mesh sieve to prepare traditional Chinese medicine powder for later use.
5. The Chinese medicinal composition for treating blood stasis type anxiety according to claim 1, wherein the supercritical fluid extraction temperature in step S2 is 60 ℃.
6. The Chinese medicinal composition for treating blood stasis type anxiety according to claim 1, wherein the supercritical fluid extraction pressure in step S2 is 30 MPa.
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| CN105998629A (en) * | 2016-07-14 | 2016-10-12 | 成都嘉宝祥生物科技有限公司 | Traditional Chinese medicine composition for treating syndrome of qi stagnation and blood stasis |
| CN106267073A (en) * | 2016-08-29 | 2017-01-04 | 谢浩峰 | A kind of pharmaceutical composition improving stagnation of QI body constitution and a kind of medicine brick and preparation method thereof |
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| CN101744918B (en) * | 2008-12-20 | 2011-07-20 | 鲁南制药集团股份有限公司 | Medicine for treating generalized anxiety and preparation method thereof |
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| CN105998629A (en) * | 2016-07-14 | 2016-10-12 | 成都嘉宝祥生物科技有限公司 | Traditional Chinese medicine composition for treating syndrome of qi stagnation and blood stasis |
| CN106267073A (en) * | 2016-08-29 | 2017-01-04 | 谢浩峰 | A kind of pharmaceutical composition improving stagnation of QI body constitution and a kind of medicine brick and preparation method thereof |
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