Method for preparing bionic skin care composition suitable for young men and women based on skin lipidomics
Technical Field
The invention relates to a method for preparing a skin care product, in particular to a method for preparing a skin care composition suitable for young men and women based on skin lipidomics, and belongs to the technical field of cosmetics.
Background
The difference in skin physiological state and structure between different sexes, such as the skin of a male being thicker, more melanin, more lipid, and more and larger pores, etc., and the skin of a female being more sensitive than that of a male, etc., can be expressed as the difference in skin surface lipid. The effect of changes in the content and composition of lipids on the skin surface on the skin condition can be divided into three areas: first, lipid changes at the skin surface can directly affect barrier function; secondly, the change of skin surface lipid can affect the metabolism of skin cells and indirectly affect the skin state; third, changes in skin surface lipids can affect skin micro-ecology and indirectly affect skin condition.
Lipidomics (lipidomics) is a discipline for comprehensively and systematically analyzing and identifying lipids in organisms, tissues or cells and molecules interacting with the lipids, understanding the structures and functions of the lipids and further disclosing the relationship between lipid metabolism and physiological and pathological processes of cells, organs and even organisms.
Lipidomics research is similar to other omics, and is to disclose the diversity lipid of a living body, the metabolic regulation and biological function thereof and further deeply explore the relationship between the lipidomics and physiological and pathological processes of cells, organs and living bodies by systematically researching lipidosome of the living bodies, tissues or cells and molecules interacting with the lipidosome by using a scale technical method.
At present, the difference of the skin surface lipid of young men and young women is not analyzed by a lipidomics method, and skin care products made based on the difference are not seen.
Disclosure of Invention
One of the purposes of the invention is to establish a method for preparing a bionic skin care composition suitable for young men and women based on skin lipidomics.
The invention also aims to provide skin care products, including cream, facial mask and the like, obtained by the method.
In order to achieve the purpose, the invention adopts the following technical means:
the invention discloses a method for preparing a bionic skin care composition suitable for young men and women based on skin lipidomics, which comprises the following steps:
a method for preparing a biomimetic skin care composition suitable for young men and women based on skin lipidomics comprises the following steps:
(1) respectively selecting 30-200 healthy volunteers of young men and women, and dividing into two groups of men and women;
(2) after cleaning the face, allowing the volunteers to sit still for 10-60 minutes in a constant temperature and humidity environment, and respectively collecting the lipid on the skin surface of each volunteer by using a tape adhesion method to serve as a sample for lipid detection;
(3) respectively recovering skin lipid in the collected samples by adopting a lipid extraction method;
(4) respectively measuring the content of each lipid component in the extracted lipid by using an instrument, and obtaining the average value of the content of each lipid component in each group of samples;
(5) respectively selecting 1-10 lipid components with high content from each group, and calculating the relative content ratio of the selected lipid components by taking the average value of the respective cholesterol content in the skin of each group as a divisor;
(6) selecting 1-10 lipids with the same or similar structure as the lipid component in step (5) from used cosmetic raw material name catalog;
(7) and (3) mixing the lipid obtained in the step (6) according to the ratio determined in the step (5) to obtain a lipid composition, and then mixing the lipid composition with a conventional skin care product matrix raw material and an auxiliary raw material to prepare the bionic skin care composition suitable for young men and women.
In the method of the present invention, preferably, the young is a population between 16 and 44 years of age.
In the method of the present invention, it is preferable that the temperature of the environment in the step (2) is 20 to 30 ℃ and the humidity is 30 to 50%.
In the method of the present invention, preferably, the skin lipids are recovered in step (3) by a Bligh-Dyer lipid extraction method.
In the method of the present invention, preferably, the apparatus in step (4) is UPLC-QTOF/MS, GC-QTOF/MS, MALDI-TOF-MS, DART-MS or DESI-MS.
In the method of the present invention, preferably, in step (6), the similar lipid refers to a lipid monomer belonging to the same lipid subclass as the lipid component selected in step (5) in LIPIDMAPS database, or a lipid mixture whose main component (content is more than 50 w/w%) belongs to the same lipid subclass as the lipid component selected in step (5) in LIPIDMAPS database, and preferably, one of the following conditions is also satisfied for the lipid monomer; (a) a difference of + -0-5 carbon atoms compared to the lipid selected in step (5); (b) the number of unsaturated bonds differs by + -0-5 from the lipid selected in step (5).
In the method of the present invention, preferably, in step (7), the matrix material includes one or a combination of two or more of oily material, powdery material and solvent-based material, and the auxiliary material includes one or a combination of two or more of surfactant, antioxidant, preservative, sunscreen agent, humectant, essence or perfume, and pigment or pigment;
wherein, preferably, the oily raw materials comprise: glycerin, coconut oil, castor oil, olive oil, palm oil, mink oil, snake oil, beef tallow, lanolin and its derivatives, carnauba wax, spermaceti wax, beeswax, liquid paraffin, vaseline, microcrystalline paraffin, squalane, silicone oil and its derivatives, fatty acids, fatty alcohols, and esters having 12 to 18 carbon atoms;
wherein, preferably, the powdery raw materials comprise: talcum powder, kaolin, zinc white powder, titanium dioxide, bentonite, zinc/magnesium stearate, calcium carbonate/magnesium and calcium hydrophosphate;
wherein, preferably, the solvent raw materials comprise: water, alcohol, isopropanol, n-butanol, acetone, butanone, diethylene glycol ethyl ether, ethyl acetate, and butyl acetate.
In the method of the present invention, preferably, in the step (7), the weight percentage of the lipid composition in the biomimetic skin care composition is 10-20%.
The invention further provides a bionic skin care composition suitable for young men and women, which is prepared by the method, and preferably, the skin care composition is cream, lotion or facial mask.
A bionic skin-care cream suitable for young men or young women is prepared by the method;
wherein, the cream suitable for young men consists of 18 percent of lipid composition and 82 percent of mixture of matrix raw materials and auxiliary raw materials according to weight percentage; wherein the lipid composition consists of caprylic triglyceride, dilauryl glyceride, jojoba oil, squalane, ceramide III, cholesterol, and jojoba ester, and the ratio of caprylic triglyceride: dilaurin glycerol: jojoba oil: squalane: ceramide III: cholesterol: weight ratio of jojoba ester 196.03: 12.75: 3.99: 9: 1.91: 1: 11; the mixture of the substrate raw material and the auxiliary raw material consists of 3 percent of glycerin, 0.3 percent of benzyl alcohol, 0.3 percent of potassium sorbate and 96.4 percent of refined water according to weight percentage;
wherein, the cream suitable for young women comprises 18 percent of lipid composition and 82 percent of mixture of matrix raw materials and auxiliary raw materials according to weight percentage; wherein the lipid composition consists of caprylic triglyceride, dilauryl glyceride, jojoba oil, squalane, ceramide III, cholesterol, and jojoba ester, and the ratio of caprylic triglyceride: dilaurin glycerol: jojoba oil: squalane: ceramide III: cholesterol: weight ratio of jojoba ester 210.13: 13.21: 4.13: 4.15: 4.41: 1: 4.3; the mixture of the matrix raw material and the auxiliary raw material comprises, by weight, 3% of glycerol, 0.3% of benzyl alcohol, 0.3% of potassium sorbate and 96.4% of refined water.
A bionic skin-care facial mask suitable for young men or young women is prepared according to the method disclosed by the invention;
the facial mask suitable for young men consists of 12% of phase A, 87.9% of phase B and 0.1% of phase C in percentage by weight, wherein the phase A is composed of caprylic triglyceride, dilauryl glyceride, jojoba oil, squalane, ceramide III, cholesterol and jojoba ester according to the weight percentage of caprylic triglyceride: dilaurin glycerol: jojoba oil: squalane: ceramide III: cholesterol: weight ratio of jojoba ester 196.03: 12.75: 3.99: 9: 1.91: 1: 11, a lipid composition obtained by mixing; the mixture of the matrix raw material and the auxiliary raw material consists of a phase B and a phase C, wherein the phase B consists of 1 w/w% of carbomer, 2 w/w% of propylene glycol, 0.4 w/w% of benzyl alcohol, 0.3 w/w% of potassium sorbate and 96.3 w/w% of refined water; the phase C is triethanolamine;
the facial mask for young women consists of 12% of phase A, 87.9% of phase B and 0.1% of phase C in percentage by weight, wherein the phase A is composed of caprylic triglyceride, dilauryl glyceride, jojoba oil, squalane, ceramide III, cholesterol and jojoba ester according to the weight percentage of caprylic triglyceride: dilaurin glycerol: jojoba oil: squalane: ceramide III: cholesterol: weight ratio of jojoba ester 210.13: 13.21: 4.13: 4.15: 4.41: 1: 4.3 lipid composition obtained by mixing; the mixture of the matrix raw material and the auxiliary raw material consists of a phase B and a phase C, wherein the phase B consists of 1 w/w% of carbomer, 2 w/w% of propylene glycol, 0.4 w/w% of benzyl alcohol, 0.3 w/w% of potassium sorbate and 96.3 w/w% of refined water; the phase C is triethanolamine.
Compared with the prior art, the invention has the beneficial effects that:
the invention takes the skin of young men and women as a research object, and screens out the lipid with higher content by analyzing the lipid components and the content on the surface of the skin with different sexes, and then adds the single component or the composite component with proper proportion into the skin care product to prepare the bionic skin care product which is suitable for the skin states with different sexes. Clinical tests prove that the skin care product prepared by the method can better meet the requirements of skins of different genders on lipid, has good water locking and moisturizing effects, does not have the phenomenon of skin discomfort, and has good application prospect.
Detailed Description
The invention will be further described with reference to specific embodiments, and the advantages and features of the invention will become apparent as the description proceeds. The examples are illustrative only and do not limit the scope of the present invention in any way. It will be understood by those skilled in the art that various changes in form and details may be made therein without departing from the spirit and scope of the invention, and that such changes and modifications may be made without departing from the spirit and scope of the invention.
Example 1 preparation of a bionic skin cream suitable for young men and women
1. Selecting 50 healthy young male and female volunteers, wherein the age is 16-44 years, and excluding skin diseases and systemic diseases, and dividing into two groups;
2. after cleaning the face (removing lipid components secreted by the skin), allowing a volunteer to sit still for 30 minutes in a constant-temperature and constant-humidity environment with the temperature of 25 ℃ and the relative humidity of 50%, sticking the volunteer to the cheek part on the right side by using an adhesive tape, taking down the adhesive tape after 3 minutes, and placing the adhesive tape in a sample tube to be used as a sample for lipid detection;
3. the lipids in the collected samples were extracted using the Bligh-Dyer lipid extraction method (Bligh EG, Dyer WJ. Canadian journal of biochemistry and physiology,1959,37(8): 911-.
4. Respectively measuring various lipid components in the extracted lipids by using UPLC-QTOF-MS, detecting 3412 lipids together, classifying the lipids, analyzing the content of various components, obtaining the average value of the content of each lipid component in each group of samples, and selecting the lipid component with higher content.
5. The lipid content of young men and women is analyzed, and the lipids with the contents in the first seven positions in the male and female groups are all triacylglycerol, diacylglycerol, fatty acid, squalene, ceramide, cholesterol and wax ester. Taking the average value of the cholesterol content in the male group as a divisor, the skin lipid content ratio of the young male with the content ranked in the first seven positions is calculated as follows: triacylglycerols: diacylglycerol: fatty acid: squalene: ceramide: cholesterol: wax ester 196.03: 12.75: 3.99: 9: 1.91: 1: 11; taking the average value of the cholesterol content in the female group as a divisor, the skin lipid content proportion of the young females with the content ranked in the first seven positions is calculated as follows: triacylglycerols: diacylglycerol: fatty acid: squalene: ceramide: cholesterol: wax ester 210.13: 13.21: 4.13: 4.15: 4.41: 1: 4.3.
6. according to the structures of the seven lipid components, selecting raw materials in the catalog of names of used cosmetic raw materials, and selecting lipid components with the same or similar structures, wherein similar lipids satisfy lipid monomers belonging to the same lipid subclass as the lipids selected in step 5 in the LIPIDMAPS database, or lipid mixtures having main components (content more than 50 w/w%) belonging to the same lipid subclass as the lipids selected in step 5 in the LIPIDMAPS database; further preferably, the lipid monomers also satisfy: (a) a difference of + - (0-5) carbon atoms compared to the lipid selected in step 5; (b) the number of unsaturated bonds differs by + (+/- (0-5) compared to the lipids selected in step 5.
Triacylglycerol is not in the catalog of names of used cosmetic raw materials, and in the LIPIDMAPS database, caprylic triglyceride and triacylglycerol belong to the same glycerin class, so caprylic triglyceride is selected to replace triacylglycerol; diacylglycerol is not in the catalog of names of used cosmetic raw materials, and in the LIPIDMAPS database, the dilaurin and the diacylglycerol belong to the same glycerolipid, so the dilaurin is selected to replace the diacylglycerol; fatty acid is not in catalog of name of used cosmetic raw materials, in LIPIDMAPS database, the main component 11-eicosenoic acid (64.4 w/w%) of jojoba oil and fatty acid belong to fatty acid, so jojoba oil is selected to replace fatty acid; alternative selection methods of other raw materials are the same: squalane instead of squalene, ceramide III instead of ceramide, cholesterol instead of cholesterol, and jojoba ester instead of wax ester.
7. The lipid composition suitable for the skin of young women is prepared from the raw materials according to the proportion of the components (in men's cream, caprylic triglyceride: dilauryl glyceride: jojoba oil: squalane: ceramide III: cholesterol: jojoba ester: 196.03: 12.75: 3.99: 9: 1.91: 1: 11; in women's cream, caprylic triglyceride: dilauryl glyceride: jojoba oil: squalane: ceramide III: cholesterol: jojoba ester: 210.13: 13.21: 4.13: 4.15: 4.41: 1: 4.3).
8. Based on the lipid composition, man cream and woman cream suitable for different genders are prepared, and the preparation method of 100g of the product is as follows:
the men's face cream comprises: taking 18g of phase A (lipid composition) and 82g of phase B (containing 3 w/w% of glycerin, 0.3 w/w% of benzyl alcohol, 0.3 w/w% of potassium sorbate and 96.4 w/w% of refined water), the preparation method comprises the following steps: heating phase A and phase B to 80 deg.C respectively, adding phase B into phase A under stirring, and slowly cooling to 60 deg.C under stirring. Homogenizing until the raw materials are completely dispersed, and cooling to room temperature to obtain the final product.
Female face cream: the preparation method of the lipid composition comprises taking 18g of phase A (lipid composition) and 82g of phase B (containing glycerol 3 w/w%, benzyl alcohol 0.3 w/w%, potassium sorbate 0.3 w/w%, and refined water 96.4 w/w%): heating phase A and phase B to 80 deg.C respectively, adding phase B into phase A under stirring, and slowly cooling to 60 deg.C under stirring. Homogenizing until the raw materials are completely dispersed, and cooling to room temperature to obtain the final product.
Example 2 preparation of a biomimetic skin care mask suitable for young men and women
1. Selecting 50 healthy young male and female volunteers, wherein the age is 16-44 years, and excluding skin diseases and systemic diseases, and dividing into two groups;
2. after cleaning the face (removing lipid components secreted by the skin), allowing a volunteer to sit still for 30 minutes in a constant-temperature and constant-humidity environment with the temperature of 25 ℃ and the relative humidity of 50%, sticking the volunteer to the cheek part on the right side by using an adhesive tape, taking down the adhesive tape after 3 minutes, and placing the adhesive tape in a sample tube to be used as a sample for lipid detection;
3. the lipids in the collected samples were extracted using the Bligh-Dyer lipid extraction method (Bligh EG, Dyer WJ. Canadian journal of biochemistry and physiology,1959,37(8): 911-.
4. Respectively measuring various lipid components in the extracted lipids by using UPLC-QTOF-MS, detecting 3412 lipids together, classifying the lipids, analyzing the content of various components, obtaining the average value of the content of each lipid component in each group of samples, and selecting the lipid component with higher content.
5. The lipid content of young men and women is analyzed, and the lipids with the contents in the first seven positions in the male and female groups are all triacylglycerol, diacylglycerol, fatty acid, squalene, ceramide, cholesterol and wax ester. Taking the average value of the cholesterol content in the male group as a divisor, the skin lipid content ratio of the young male with the content ranked in the first seven positions is calculated as follows: triacylglycerols: diacylglycerol: fatty acid: squalene: ceramide: cholesterol: wax ester 196.03: 12.75: 3.99: 9: 1.91: 1: 11; taking the average value of the cholesterol content in the female group as a divisor, the skin lipid content proportion of the young females with the content ranked in the first seven positions is calculated as follows: triacylglycerols: diacylglycerol: fatty acid: squalene: ceramide: cholesterol: wax ester 210.13: 13.21: 4.13: 4.15: 4.41: 1: 4.3.
6. according to the structures of the seven lipid components, selecting raw materials in the catalog of names of used cosmetic raw materials, and selecting lipid components with the same or similar structures, wherein similar lipids satisfy lipid monomers belonging to the same lipid subclass as the lipids selected in step 5 in the LIPIDMAPS database, or lipid mixtures having main components (content more than 50 w/w%) belonging to the same lipid subclass as the lipids selected in step 5 in the LIPIDMAPS database; further preferably, the lipid monomers also satisfy: (a) a difference of + - (0-5) carbon atoms compared to the lipid selected in step 5; (b) the number of unsaturated bonds differs by + (+/- (0-5) compared to the lipids selected in step 5.
Triacylglycerol is not in the catalog of names of used cosmetic raw materials, and in the LIPIDMAPS database, caprylic triglyceride and triacylglycerol belong to the same glycerin class, so caprylic triglyceride is selected to replace triacylglycerol; diacylglycerol is not in the catalog of names of used cosmetic raw materials, and in the LIPIDMAPS database, the dilaurin and the diacylglycerol belong to the same glycerolipid, so the dilaurin is selected to replace the diacylglycerol; fatty acid is not in catalog of name of used cosmetic raw materials, in LIPIDMAPS database, the main component 11-eicosenoic acid (64.4 w/w%) of jojoba oil and fatty acid belong to fatty acid, so jojoba oil is selected to replace fatty acid; alternative selection methods of other raw materials are the same: squalane instead of squalene, ceramide III instead of ceramide, cholesterol instead of cholesterol, and jojoba ester instead of wax ester.
7. The lipid composition suitable for the skin of young men and women is prepared from the raw materials according to the proportion of the components (in a facial mask for men, caprylic triglyceride: diglycerol dilaurate: jojoba oil: squalane: ceramide III: cholesterol: jojoba ester: 196.03: 12.75: 3.99: 9: 1.91: 1: 11; in a facial mask for women, caprylic triglyceride: diglycerol dilaurate: jojoba oil: squalane: ceramide III: cholesterol: jojoba ester: 210.13: 13.21: 4.13: 4.15: 4.41: 1: 4.3).
8. Based on the lipid composition, a man mask and a woman mask suitable for young men and young women are prepared, and the preparation method of 100g of the product is as follows:
the male mask comprises: 12g of phase A (lipid composition suitable for men), 87.9g of phase B (containing 1 w/w% of carbomer, 2 w/w% of propylene glycol, 0.4 w/w% of benzyl alcohol, 0.3% w/w% of potassium sorbate, 96.3 w/w% of refined water) and 0.1g of triethanolamine phase C. The preparation method comprises the following steps: combine phase a with mixing and heat to 70 ℃. Mixing phase B components until uniform, and heating to 70 deg.C. Phase B was added to phase A with moderate speed stirring. And neutralizing the mixture by using the phase C, and continuously stirring and cooling to room temperature to obtain the compound.
Female mask: 12g of phase A (lipid composition suitable for ladies), 87.9g of phase B (containing 1 w/w% of carbomer, 2 w/w% of propylene glycol, 0.4 w/w% of benzyl alcohol, 0.3% w/w% of potassium sorbate, 96.3 w/w% of refined water) and 0.1g of triethanolamine phase C. The preparation method comprises the following steps: combine phase a with mixing and heat to 70 ℃. Mixing phase B components until uniform, and heating to 70 deg.C. Phase B was added to phase A with moderate speed stirring. And neutralizing the mixture by using the phase C, and continuously stirring and cooling to room temperature to obtain the compound.
Example 3 efficacy testing and clinical testing of skin care compositions of the present invention
Experiment one:
the test was conducted in three stages using three skin care products tested, each stage lasting 4 weeks, with 40 selected from the young male and female healthy volunteers of example 1, and after one stage was completed, the skin care products tested were discontinued for 28 days and the test was conducted in the next stage.
The three skin care products tested were as follows:
skin care product to be tested 1: man or woman's cream prepared according to the method of example 1
Skin care product 2: prepared according to the method of example 1, except that no lipid composition is added
Skin care product to be tested 3: prepared according to the method of example 1, except that the lipid composition in example 1 was replaced with the following lipid composition:
polydimethylsiloxane, stearic acid, isononyl isononanoate, jojoba oil, Ve acetate, cetostearyl alcohol, paraffin and hydrogenated polydecene, wherein the ratio of the components is as follows: 1.0: 0.1: 1.5: 3.0: 3.0: 5.0: 3.0: 0.3.
the method comprises the following steps:
the first stage is as follows: after cleansing the skin in the morning and evening every day, young males and young females of the test group applied the male cream and female cream of the test skin care product 1 to the facial skin, respectively. The water content of the stratum corneum and the transdermal water loss were measured on days 7, 14, 21 and 28, respectively, and the results were statistically evaluated according to the subjective evaluation of volunteers. After the first phase, the skin care product was removed from service for 28 days and the second phase of the experiment was performed.
And a second stage: after cleansing the skin in the morning and evening each day, young males and young females of the test group each applied the test skin care product 2 to the facial skin. The water content of the stratum corneum and the transdermal water loss were measured on days 7, 14, 21 and 28, respectively, and the results were statistically evaluated according to the subjective evaluation of volunteers. After the second phase, the skin care product to be tested was stopped for 28 days, and the experiment was performed in the third phase.
And a third stage: after cleansing the skin in the morning and evening each day, young males and young females of the test group each applied the test skin care product 3 to the facial skin. The water content of the stratum corneum and the transdermal water loss were measured on days 7, 14, 21 and 28, respectively, and the results were statistically evaluated according to the subjective evaluation of volunteers.
And (4) judging the standard: measurement of water content of skin stratum corneum, amount of water loss through skin, and subjective feeling (slight improvement, significant improvement, no improvement, skin discomfort).
As a result:
results of use of skin care product 1 tested: 1) after 2 weeks, the skin stratum corneum water content of both young male volunteers and young female volunteers is remarkably increased, the transdermal water loss is remarkably reduced, 51% of young male and female volunteers have remarkable improvement on subjective feeling of the skin state, 18% of young male and female volunteers have slight improvement on subjective feeling of the skin state, and 31% of young male and female volunteers have no improvement on subjective feeling, no skin discomfort and the like; 2) after 4 weeks, the skin stratum corneum water content of both young male volunteers and young female volunteers is remarkably increased, the transdermal water loss is remarkably reduced, 63% of young male and female volunteers have remarkable improvement in subjective feeling of skin state, 27% of young male and female volunteers have slight improvement in subjective feeling of skin state, and 10% of young male and female volunteers have no improvement in subjective feeling, no skin discomfort and the like.
Results of use of skin care product 2 tested: 1) after 2 weeks, the skin stratum corneum water content and the transdermal water loss of the young male and female volunteers have no obvious change, 11 percent of the young male and female volunteers have slight improvement on the subjective feeling of the skin, 89 percent of the young male and female volunteers have no improvement on the subjective feeling, no skin discomfort and the like; 2) after 4 weeks, the skin stratum corneum water content and the transdermal water loss of young men and women have no obvious change, 14 percent of young men and women volunteers subjectively feel that the skin state is slightly improved, and 86 percent of young men and women volunteers subjectively feel that the skin state is not improved, and the phenomena of skin discomfort and the like do not exist.
Results of use of skin care product 3 tested: 1) after 2 weeks, the skin stratum corneum water content and the transdermal water loss of young men and women have no obvious change, 10 percent of young men and women volunteers subjectively feel the skin state to be obviously improved, 15 percent of young men and women volunteers subjectively feel the skin state to be slightly improved, 75 percent of young men and women volunteers subjectively feel to be not improved, and the skin discomfort does not exist; 2) after 4 weeks, the skin stratum corneum water content of the young male and female volunteers is increased, the transdermal water loss is slightly reduced, 16% of the young male and female volunteers have remarkable improvement on subjective feeling of the skin state, 14% of the young male and female volunteers have slight improvement on subjective feeling of the skin state, and 70% of the young male and female volunteers have no improvement on subjective feeling, no skin discomfort and the like.
Experiment two:
the test was conducted in three stages using three skin care products tested, each stage lasting 4 weeks, with 40 selected from healthy young male and female volunteers of example 1, and after one stage was completed, the skin care products tested were discontinued for 28 days and the test was conducted in the next stage.
The three skin care products tested were as follows:
skin care product tested 4: man mask and woman mask prepared according to the method of example 2
Skin care product to be tested 5: prepared according to the method of example 2, except that no lipid composition is added
Test skin care product 6: prepared according to the method of example 2, except that the lipid composition in example 2 was replaced with the following lipid composition:
polydimethylsiloxane, stearic acid, isononyl isononanoate, jojoba oil, Ve acetate, cetostearyl alcohol, paraffin and hydrogenated polydecene, wherein the ratio of the components is as follows: 1.0: 0.1: 1.5: 3.0: 3.0: 5.0: 3.0: 0.3.
the method comprises the following steps:
the first stage is as follows: after cleansing the skin in the morning and evening every day, young men and young women of the test group used the male mask and female mask of the test skin care product 4 on the facial skin, respectively. The water content of the stratum corneum and the transdermal water loss were measured on days 7, 14, 21 and 28, respectively, and the results were statistically evaluated according to the subjective evaluation of volunteers. After the first phase, the skin care product was removed from service for 28 days and the second phase of the experiment was performed.
And a second stage: after cleansing the skin in the morning and evening each day, young males and young females of the test group each applied the test skin care product 5 to the facial skin. The water content of the stratum corneum and the transdermal water loss were measured on days 7, 14, 21 and 28, respectively, and the results were statistically evaluated according to the subjective evaluation of volunteers. After the second phase, the skin care product to be tested was stopped for 28 days, and the experiment was performed in the third phase.
And a third stage: after cleansing the skin in the morning and evening each day, young males and young females of the test group each applied the test skin care product 6 to the facial skin. The water content of the stratum corneum and the transdermal water loss were measured on days 7, 14, 21 and 28, respectively, and the results were statistically evaluated according to the subjective evaluation of volunteers.
And (4) judging the standard: measurement of water content of skin stratum corneum, amount of water loss through skin, and subjective feeling (slight improvement, significant improvement, no improvement, skin discomfort).
As a result:
results of use of skin care product 4 tested: 1) after 2 weeks, the skin stratum corneum water content of both young male volunteers and young female volunteers is remarkably increased, the transdermal water loss is remarkably reduced, 56% of young male and female volunteers subjectively feel that the skin state is remarkably improved, 18% of young male and female volunteers subjectively feel that the skin state is slightly improved, 26% of young male and female volunteers subjectively feel that the skin is not improved, and the skin is not uncomfortable; 2) after 4 weeks, the skin stratum corneum water content of the young male and female volunteers is obviously increased, the transdermal water loss is obviously reduced, 69% of the young male and female volunteers subjectively feel the skin state to be obviously improved, 24% of the young male and female volunteers subjectively feel the skin state to be slightly improved, and 7% of the young male and female volunteers subjectively feel to be not improved and have no phenomena of skin discomfort and the like.
Results of use of the skin care product 5 tested: 1) after 2 weeks, the skin stratum corneum water content and the transdermal water loss of the young male and female volunteers have no obvious change, 10 percent of the young male and female volunteers subjectively feel that the skin state is slightly improved, and 90 percent of the young male and female volunteers subjectively feel that the skin state is not improved, and the phenomena of skin discomfort and the like do not exist; 2) after 4 weeks, the skin stratum corneum water content and the transdermal water loss of young male and female volunteers have no obvious change, 16 percent of volunteers subjectively feel that the skin state is slightly improved, and 84 percent of volunteers subjectively feel that the skin state is not improved, and the phenomena of skin discomfort and the like do not exist.
Results of use of the skin care product 6 tested: 1) after 2 weeks, the skin stratum corneum water content and the transdermal water loss of the young male and female volunteers have no obvious change, 13 percent of the young male and female volunteers have obvious improvement on the subjective feeling of the skin state, 19 percent of the young male and female volunteers have slight improvement on the subjective feeling of the skin state, and 68 percent of the young male and female volunteers have no improvement on the subjective feeling, no skin discomfort and the like; 2) after 4 weeks, the skin stratum corneum water content of the young male and female volunteers is increased, the transdermal water loss is slightly reduced, 18% of the young male and female volunteers have remarkable improvement on subjective feeling of the skin state, 17% of the young male and female volunteers have slight improvement on subjective feeling of the skin state, and 65% of the young male and female volunteers have no improvement on subjective feeling, no skin discomfort and the like.