CN107653266B - 含鳞翅目病毒增强子的重组表达载体在双翅目昆虫细胞中表达蛋白的应用 - Google Patents
含鳞翅目病毒增强子的重组表达载体在双翅目昆虫细胞中表达蛋白的应用 Download PDFInfo
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- CN107653266B CN107653266B CN201610602292.5A CN201610602292A CN107653266B CN 107653266 B CN107653266 B CN 107653266B CN 201610602292 A CN201610602292 A CN 201610602292A CN 107653266 B CN107653266 B CN 107653266B
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- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
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- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/705—Receptors; Cell surface antigens; Cell surface determinants
- C07K14/715—Receptors; Cell surface antigens; Cell surface determinants for cytokines; for lymphokines; for interferons
- C07K14/7151—Receptors; Cell surface antigens; Cell surface determinants for cytokines; for lymphokines; for interferons for tumor necrosis factor [TNF], for lymphotoxin [LT]
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- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/30—Non-immunoglobulin-derived peptide or protein having an immunoglobulin constant or Fc region, or a fragment thereof, attached thereto
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- C12N2800/00—Nucleic acids vectors
- C12N2800/10—Plasmid DNA
- C12N2800/103—Plasmid DNA for invertebrates
- C12N2800/105—Plasmid DNA for invertebrates for insects
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- C12N2830/00—Vector systems having a special element relevant for transcription
- C12N2830/001—Vector systems having a special element relevant for transcription controllable enhancer/promoter combination
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- C12N2830/00—Vector systems having a special element relevant for transcription
- C12N2830/60—Vector systems having a special element relevant for transcription from viruses
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Priority Applications (1)
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CN201610602292.5A CN107653266B (zh) | 2016-07-26 | 2016-07-26 | 含鳞翅目病毒增强子的重组表达载体在双翅目昆虫细胞中表达蛋白的应用 |
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CN201610602292.5A CN107653266B (zh) | 2016-07-26 | 2016-07-26 | 含鳞翅目病毒增强子的重组表达载体在双翅目昆虫细胞中表达蛋白的应用 |
Publications (2)
Publication Number | Publication Date |
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CN107653266A CN107653266A (zh) | 2018-02-02 |
CN107653266B true CN107653266B (zh) | 2020-07-14 |
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CN201610602292.5A Active CN107653266B (zh) | 2016-07-26 | 2016-07-26 | 含鳞翅目病毒增强子的重组表达载体在双翅目昆虫细胞中表达蛋白的应用 |
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Families Citing this family (3)
Publication number | Priority date | Publication date | Assignee | Title |
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PT115073A (pt) | 2018-10-09 | 2020-05-29 | Inst De Biologia Molecular E Celular Ibmc | Ácido nucleico para ativar a expressão e a produção de proteínas |
CN113430227A (zh) * | 2020-03-23 | 2021-09-24 | 佛山汉腾生物科技有限公司 | 制备稳定细胞池的方法、蛋白表达方法及试剂盒 |
CN113234755B (zh) * | 2021-03-31 | 2023-05-02 | 浙江省农业科学院 | 一种罗氏沼虾外源基因的表达质粒及其转录表达方法 |
Citations (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN105238816A (zh) * | 2015-10-26 | 2016-01-13 | 广州晓经生物技术有限公司 | 一种表达载体 |
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Patent Citations (1)
Publication number | Priority date | Publication date | Assignee | Title |
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CN105238816A (zh) * | 2015-10-26 | 2016-01-13 | 广州晓经生物技术有限公司 | 一种表达载体 |
Non-Patent Citations (2)
Title |
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Glucocorticoid‐inducible gene expression vectors for use in Drosophila melanogaster Schneider 2 cells;J Poels等;《Insect Molecular Biology》;20041231;第13卷(第2期);第205-211页 * |
hycu-hr6, A large homologous region of the Hyphantria cunea nucleopolyhedrovirus genome, as a powerful and versatile enhancer in insect expression systems;Cristiano A等;《Virus Genes》;20091231;第39卷(第3期);第403-408页 * |
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Effective date of registration: 20191203 Address after: Room 511, floor 5, building a, Central Europe center, No. 2, South Lingnan Avenue, Lecong Town, Shunde District, Foshan City, Guangdong Province Applicant after: Foshan hanteng Biotechnology Co., Ltd Applicant after: Cantonbio Co., Ltd. Address before: 510000 the third layer 302 unit of the International Biological Island spiral three road 12, Guangzhou Development Zone, Guangzhou, Guangdong Applicant before: Cantonbio Co., Ltd. |
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Denomination of invention: Application of recombinant expression vector containing Lepidoptera virus enhancer in Diptera Insect Cells Effective date of registration: 20210120 Granted publication date: 20200714 Pledgee: Shunde Guangdong rural commercial bank Limited by Share Ltd. Daliang branch Pledgor: CANTONBIO Co.,Ltd.|Foshan hanteng Biotechnology Co.,Ltd. Registration number: Y2021980000562 |
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