CN107648276A - A kind of process of low temperature ultrasonic cracking extraction giant salamander mucus active ingredient - Google Patents
A kind of process of low temperature ultrasonic cracking extraction giant salamander mucus active ingredient Download PDFInfo
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- CN107648276A CN107648276A CN201710896523.2A CN201710896523A CN107648276A CN 107648276 A CN107648276 A CN 107648276A CN 201710896523 A CN201710896523 A CN 201710896523A CN 107648276 A CN107648276 A CN 107648276A
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- giant salamander
- salamander mucus
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
- A61K35/56—Materials from animals other than mammals
- A61K35/65—Amphibians, e.g. toads, frogs, salamanders or newts
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Abstract
It is as follows that the present invention provides a kind of process concrete technology step of efficient low temperature ultrasonic cracking extraction giant salamander mucus active ingredient：(1)：By giant salamander mucus, machinery shreds under cryogenic；(2)：Giant salamander mucus particle is ground into uniform slurry through colloid mill；(3)：Fully dispersed giant salamander mucus slurry is added in Extraction medium, is put into low-temperature circulating ultrasonic extraction instrument and carries out cracking extraction, be completely dissolved in Extraction medium；(4)：Giant salamander mucus extract solution in the step (3) is used into high-speed low temperature centrifugal treating technology, ensures that the insoluble matter in extract solution is all precipitated；(5)：The centrifuged supernatant in the step (4) is taken, is molded in being freeze-dried in freeze drier.The present invention maintains the bioactive substance of giant salamander mucus, and Oxidative inactivation, extraction process time is not short, it is only necessary to which dozens of minutes completes extraction；The active ingredient free from admixture extracted, no precipitation, insoluble impurities is effectively removed, ensure active ingredient quality.
Description
Technical field
The present invention relates to field of Chinese herbal, and in particular to cosmetic field, more particularly to a kind of giant salamander mucus active ingredient
Process for extracting.
Background technology
Giant salamander is exactly traditional medicinal animal since ancient times,《Compendium of Materia Medica》、《Draft is picked up any lost article from the road》Etc. being retouched in pharmacopeia
State.The traditional Chinese medical science thinks that giant salamander property is sweet flat, there is tonifying Qi, blood-nourishing, intelligence development, nourishing, strong and other effects.Neurasthenia is cured mainly, it is physically weak after being ill,
Anaemia, dysentery etc..The skin of giant salamander, muscle, internal organs, bone, mucus etc. can be used as medicine, it is seen that its status in Chinese pharmacology.
The glutinous glycoprotein of giant salamander skin mucus gland cell release combines to form mucus with water, is covered in epithelium free surface, shape
The protective barrier natural into one.Mucus contains glutinous glycoprotein, oligosaccharide peptide, inorganic salts and " Magainin " isoreactivity thing
Matter, compared with other animal body surface mucus, giant salamander skin mucus polyoses content is up to 27.5%.Mucus glycoprotein is not only cell membrane
The important active substances of signal transduction are carried out with the important composition composition of intercellular matrix, and organism.In biological evolution
Cheng Zhong, giant salamander skin mucus, which has, resists the abundant bioactivity such as environmental change, antibacterial, antifatigue, anti-oxidant.
Glycoprotein is a kind of associated proteins, while has some properties of polysaccharide and protein, mostly water-soluble and dilute
In acid, diluted alkaline and enzymolysis liquid, therefore can be according to demand using different solvent extraction separation glycoprotein.But in all extraction separation
These steps in it should be noted that must assure that the integrality of glycoprotein large biological molecule, prevent high temperature, peracid, cross alkali and acutely
Mechanism etc. destroys the molecular structure of glycoprotein and causes the loss of bioactivity of products therefrom.Use alkali carries mostly at present
Method, water extraction, sour formulation and enzymatic isolation method extract to glycoprotein in giant salamander skin mucus, in these methods or have high temperature or
There is peracid, cross the possibility of alkali, there is the possibility for destroying glycoprotein activity, therefore invent one kind and participated in without high temperature, anacidity, alkali-free
Extracting method is just very necessary, can both ensure the leaching of bioactive substance in giant salamander mucus, ensure that biological active matter
Matter not Oxidative inactivation in extraction process, improve the quality and yield of giant salamander mucus extract.
The content of the invention
In view of the shortcomings of the prior art, it is an object of the present invention to provide a kind of low temperature ultrasonic to crack extraction giant salamander mucus
The process of active ingredient.
To reach above-mentioned purpose, the technical solution adopted by the present invention is：
A kind of process of low temperature ultrasonic cracking extraction giant salamander mucus active ingredient, concrete technology step are as follows：
(1), by giant salamander mucus, machinery shreds under cryogenic；
(2) giant salamander mucus particle, is ground into uniform slurry through colloid mill；
(3), fully dispersed giant salamander mucus slurry is added in Extraction medium, weight ratio is 1：10-30, it is put into low temperature and follows
Cracking extraction is carried out in ring ultrasonic extraction instrument, the active material of giant salamander mucus is fully discharged, is completely dissolved in Extraction medium
In；
(4) the giant salamander mucus extract solution in the step (3), is used into high-speed low temperature centrifugal treating technology, ensures extraction
Insoluble matter in liquid is all precipitated, and fresh giant salamander mucus is prepared into mucus slurry, is ensured raw in leaching process
The stability of active substances；
(5) centrifuged supernatant in the step (4), is taken, is molded in being freeze-dried in freeze drier.
Further, the cryogenic conditions in the step (1) are less than 18 DEG C.
Further, the Extraction medium in the step (2) is pure water.
Further, the cracking in the step (3) is extracted as taking ultrasonic degradation technology, makes macromolecular in giant salamander mucus
Protein cleavage is into small molecule active glycoprotein peptide.
Further, the cracking extraction in the step (3), Extracting temperature is less than 10 DEG C, and ultrasonic frequency is 20KHz,
Intensity is 100-1000W, and processing time is 10-60 minutes.
Further, the high-speed low temperature centrifugal treating technology in the step (4) is, below 10 DEG C under low temperature environment,
Rotating speed 10000rpm above centrifugal treatings 10-30min.
Further, the freeze-drying shaping in the step (5), ensure that the biology in giant salamander mucus is living to greatest extent
Property material does not aoxidize when being separated with Extraction medium, avoids active material from inactivating.
Utilization based on above-mentioned technical proposal, the present invention have advantages below compared with prior art：
1st, the present invention can keep the active nutrient component of giant salamander mucus to greatest extent, overcome traditional alkaline extraction, water
The high temperature such as formulation, sour formulation and enzymatic isolation method, peracid, the influence for crossing alkali etc., the problems such as causing active material to inactivate；
2nd, it is short the time required to extraction process of the present invention, it is only necessary to which that dozens of minutes can be completed to extract, and common extraction process
The time of at least several hours, avoid and be heated, destroyed by other stimulations etc. for active component for a long time；
3rd, the present invention extracts giant salamander mucus free from admixture, without precipitation because using hypervelocity low-temperature centrifugation treatment technology,
Ensure that the insoluble matter in extract solution is all precipitated, the quality of giant salamander mucus active ingredient has been effectively ensured.
Embodiment
The specific reality of the process of extraction giant salamander mucus active ingredient is cracked to low temperature ultrasonic disclosed by the invention below
The mode of applying elaborates, and is not used to limit the scope of the present invention.
The process of low temperature ultrasonic cracking extraction giant salamander mucus active ingredient of the present invention, concrete technology step is such as
Under：
(1), by giant salamander mucus, machinery shreds under cryogenic, it is preferable that cryogenic conditions are less than 18 DEG C；
(2) giant salamander mucus particle, is ground into uniform slurry through colloid mill；
(3), fully dispersed giant salamander mucus slurry is added in Extraction medium (pure water), weight ratio is 1:10-30, it is put into
Cracking extraction is carried out in low-temperature circulating ultrasonic extraction instrument, to take ultrasonic degradation technology, splits high molecular weight protein in giant salamander mucus
The active material of giant salamander mucus is fully discharged, is completely dissolved in Extraction medium, carried into small molecule active glycoprotein peptide by solution
It is less than 10 DEG C to take temperature, and ultrasonic frequency is 20KHz, and intensity 100-1000W, processing time is 10-60 minutes；
(4) the giant salamander mucus extract solution in step (3), is used into high-speed low temperature centrifugal treating technology, it is low below 10 DEG C
Under warm environment, rotating speed 10000rpm above centrifugal treating 10-30min, ensure that the insoluble matter in extract solution is all precipitated,
So that fresh giant salamander mucus is prepared into mucus slurry, ensure the stability of bioactive substance in leaching process.
(5) centrifuged supernatant in step (4), is taken, in being freeze-dried shaping in freeze drier, is ensured to greatest extent
Bioactive substance in giant salamander mucus does not aoxidize when being separated with Extraction medium, avoids active material from inactivating.
The preferred embodiments of the present invention are the foregoing is only, the numerical value and number mentioned in the description of description above
Value scope is not intended to limit the invention, and is simply provided preferred embodiment for the present invention, is not intended to limit the invention, right
For those skilled in the art, the present invention can have various modifications and variations.Within the spirit and principles of the invention,
Any modification, equivalent substitution and improvements made etc., should be included in the scope of the protection.
Claims (7)
1. a kind of process of low temperature ultrasonic cracking extraction giant salamander mucus active ingredient, concrete technology step are as follows：
(1), by giant salamander mucus, machinery shreds under cryogenic；
(2) giant salamander mucus particle, is ground into uniform slurry through colloid mill；
(3), fully dispersed giant salamander mucus slurry is added in Extraction medium, weight ratio is 1：10-30, it is put into low-temperature circulating and surpasses
Cracking extraction is carried out in sound abstraction instrument, the active material of giant salamander mucus is fully discharged, is completely dissolved in Extraction medium；
(4) the giant salamander mucus extract solution in the step (3), is used into high-speed low temperature centrifugal treating technology, ensured in extract solution
Insoluble matter be all precipitated, fresh giant salamander mucus is prepared into mucus slurry, ensures that biology is living in leaching process
The stability of property material；
(5) centrifuged supernatant in the step (4), is taken, is molded in being freeze-dried in freeze drier.
2. the process of low temperature ultrasonic cracking extraction giant salamander mucus active ingredient as claimed in claim 1, it is characterised in that
Cryogenic conditions in the step (1) are less than 18 DEG C.
3. the process of low temperature ultrasonic cracking extraction giant salamander mucus active ingredient as claimed in claim 1 or 2, its feature exist
In the Extraction medium in the step (2) is pure water.
4. the process of low temperature ultrasonic cracking extraction giant salamander mucus active ingredient as claimed in claim 1 or 2, its feature exist
In the cracking in the step (3) is extracted as taking ultrasonic degradation technology, high molecular weight protein in giant salamander mucus is cracked into small point
Sub- active glucoprotein peptide.
5. the process of low temperature ultrasonic cracking extraction giant salamander mucus active ingredient as claimed in claim 4, it is characterised in that
Cracking extraction in the step (3), Extracting temperature is less than 10 DEG C, and ultrasonic frequency is 20KHz, intensity 100-1000W,
Processing time is 10-60 minutes.
6. the process of low temperature ultrasonic cracking extraction giant salamander mucus active ingredient as claimed in claim 1 or 2, its feature exist
In, the high-speed low temperature centrifugal treating technology in the step (4) is, below 10 DEG C under low temperature environment, rotating speed 10000rpm with
Upper centrifugal treating 10-30min.
7. the process of low temperature ultrasonic cracking extraction giant salamander mucus active ingredient as claimed in claim 1 or 2, its feature exist
In the freeze-drying shaping in the step (5), the bioactive substance in guarantee giant salamander mucus is with extracting Jie to greatest extent
Matter does not aoxidize when separating, and avoids active material from inactivating.
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Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103613654A (en) * | 2013-09-03 | 2014-03-05 | 张家界（中国）金驰大鲵生物科技有限公司 | Giant salamander mucinous protein, preparation method and applications in cosmetics |
| CN104357522A (en) * | 2014-11-11 | 2015-02-18 | 四川龙王洞生态农业开发有限公司 | Method for extracting collagen by using ecdysis of giant salamander |
| CN105287697A (en) * | 2015-10-23 | 2016-02-03 | 陕西龙香堂生物工程股份有限公司 | Andrias davidianus tissue extract compound composition and application thereof |
| CN105769926A (en) * | 2016-02-28 | 2016-07-20 | 汉中领维生物科技发展有限公司 | Skin mucus extracts of andrias davidianus Blanchard for preparing anti-breast cancer drugs and application thereof |
| CN107200781A (en) * | 2017-06-27 | 2017-09-26 | 贵州工程应用技术学院 | A kind of method that collagen is extracted from giant salamander |
-
2017
- 2017-09-28 CN CN201710896523.2A patent/CN107648276A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103613654A (en) * | 2013-09-03 | 2014-03-05 | 张家界（中国）金驰大鲵生物科技有限公司 | Giant salamander mucinous protein, preparation method and applications in cosmetics |
| CN104357522A (en) * | 2014-11-11 | 2015-02-18 | 四川龙王洞生态农业开发有限公司 | Method for extracting collagen by using ecdysis of giant salamander |
| CN105287697A (en) * | 2015-10-23 | 2016-02-03 | 陕西龙香堂生物工程股份有限公司 | Andrias davidianus tissue extract compound composition and application thereof |
| CN105769926A (en) * | 2016-02-28 | 2016-07-20 | 汉中领维生物科技发展有限公司 | Skin mucus extracts of andrias davidianus Blanchard for preparing anti-breast cancer drugs and application thereof |
| CN107200781A (en) * | 2017-06-27 | 2017-09-26 | 贵州工程应用技术学院 | A kind of method that collagen is extracted from giant salamander |
Non-Patent Citations (4)
| Title |
|---|
| 李伟等: "大鲵黏液酶解产物的制备及其抗疲劳作用的研究", 《食品工业科技》 * |
| 胡代花: "大鲵生物活性肽和酶解产物研究及应用进展", 《黑龙江畜牧兽医》 * |
| 金文刚等: "大鲵皮明胶提取及其性质分析", 《食品发酵工业》 * |
| 陈德经等: "大鲵皮肤粘液多糖的提取及单糖组成分析", 《天然产物研究与开发》 * |
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Application publication date: 20180202 |